##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049362_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4328772 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.30210507737529 31.0 31.0 34.0 27.0 34.0 2 30.567533933411138 31.0 31.0 34.0 27.0 34.0 3 30.76459952152712 31.0 31.0 34.0 27.0 34.0 4 34.39811475402262 37.0 35.0 37.0 31.0 37.0 5 34.4254520681616 37.0 35.0 37.0 31.0 37.0 6 34.41132704609991 37.0 35.0 37.0 31.0 37.0 7 34.30807790292489 37.0 35.0 37.0 30.0 37.0 8 34.264452828654406 37.0 35.0 37.0 30.0 37.0 9 35.85163575258757 39.0 37.0 39.0 30.0 39.0 10-11 35.80384390769484 39.0 37.0 39.0 30.0 39.0 12-13 35.733550531189906 39.0 37.0 39.0 30.0 39.0 14-15 36.88384338560682 40.0 37.0 41.0 30.0 41.0 16-17 36.84057279986102 40.0 37.0 41.0 30.0 41.0 18-19 36.86679894436575 40.0 37.0 41.0 30.0 41.0 20-21 36.93156396317478 40.0 37.0 41.0 30.0 41.0 22-23 36.87803815955195 40.0 37.0 41.0 30.0 41.0 24-25 36.87284777299428 40.0 37.0 41.0 30.0 41.0 26-27 36.8486118002981 40.0 37.0 41.0 30.0 41.0 28-29 36.78729452602262 40.0 37.0 41.0 30.0 41.0 30-31 36.72026835786223 40.0 37.0 41.0 30.0 41.0 32-33 36.65650281419303 40.0 37.0 41.0 30.0 41.0 34-35 36.5566530184542 40.0 36.5 41.0 30.0 41.0 36-37 36.46743048605933 40.0 36.0 41.0 30.0 41.0 38-39 36.35480697528075 40.0 36.0 41.0 30.0 41.0 40-41 36.2440725683866 40.0 36.0 41.0 29.0 41.0 42-43 36.10039798815923 40.0 36.0 41.0 29.0 41.0 44-45 35.95938559480611 40.0 35.5 41.0 28.0 41.0 46-47 35.826504260330644 39.0 35.0 41.0 28.0 41.0 48-49 35.692267460610076 39.0 35.0 41.0 28.0 41.0 50-51 35.55372874801445 39.0 35.0 41.0 27.0 41.0 52-53 35.40114829332661 39.0 35.0 41.0 27.0 41.0 54-55 35.26229551937594 39.0 35.0 40.5 27.0 41.0 56-57 35.129711844375265 39.0 35.0 40.0 26.0 41.0 58-59 34.92894497561895 38.5 34.0 40.0 26.0 41.0 60-61 34.708668878841394 38.0 34.0 40.0 26.0 41.0 62-63 34.49080270339948 38.0 34.0 40.0 26.0 41.0 64-65 34.24934900706251 37.5 34.0 40.0 25.0 41.0 66-67 33.98895552826529 37.0 34.0 40.0 24.5 41.0 68-69 33.70236836220526 36.5 33.0 40.0 24.0 41.0 70-71 33.42043020514825 36.0 33.0 39.0 24.0 41.0 72-73 33.08086046111923 36.0 33.0 39.0 23.0 41.0 74-75 32.737141387904 35.0 33.0 39.0 22.0 40.0 76-77 32.40998844475985 35.0 32.0 37.5 22.0 40.0 78-79 32.094564694098004 35.0 32.0 37.0 21.0 39.0 80-81 31.74678707956899 35.0 32.0 37.0 20.0 39.0 82-83 31.438201064874747 35.0 32.0 36.0 19.5 38.5 84-85 31.15309595885392 35.0 32.0 36.0 18.0 37.0 86-87 30.839753976416404 35.0 31.5 35.5 16.5 37.0 88-89 30.588956059593805 35.0 31.0 35.0 12.5 36.5 90-91 30.391231277600205 35.0 31.0 35.0 8.5 36.0 92-93 30.157706157774076 34.5 31.0 35.0 3.5 36.0 94-95 29.9501303140937 34.0 31.0 35.0 2.0 36.0 96-97 29.693865257860658 34.0 31.0 35.0 2.0 35.5 98-99 29.487207341943627 34.0 31.0 35.0 2.0 35.0 100 29.36138262768286 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3201720303242368 1101 2 0.296598545936579 1101 3 0.3728819395076428 1101 4 0.3941548210788497 1101 5 0.4587901284721809 1101 6 0.556429908489271 1101 7 0.4597510872702628 1101 8 0.510991595036927 1101 9 0.6402511995756583 1101 10-11 0.6774628807815688 1101 12-13 0.7030915117826879 1101 14-15 0.78956524487905 1101 16-17 0.7681761678211743 1101 18-19 0.7064974220666116 1101 20-21 0.6999466930941765 1101 22-23 0.7173828506183213 1101 24-25 0.6981263473521722 1101 26-27 0.60810084710144 1101 28-29 0.6580338244544066 1101 30-31 0.6929841681735383 1101 32-33 0.6484322001402987 1101 34-35 0.7017468049823279 1101 36-37 0.7183134533763607 1101 38-39 0.7821520846854781 1101 40-41 0.7844150833794004 1101 42-43 0.6966406171605968 1101 44-45 0.6361240592170105 1101 46-47 0.6680065959885226 1101 48-49 0.5767060117595761 1101 50-51 0.5809189205922181 1101 52-53 0.616339693649337 1101 54-55 0.6169503480304215 1101 56-57 0.5557367809999931 1101 58-59 0.7227157962621433 1101 60-61 0.6290182718098905 1101 62-63 0.6192726951649732 1101 64-65 0.6275709201500916 1101 66-67 0.6771960052261434 1101 68-69 0.6598241409773351 1101 70-71 0.7220451689627012 1101 72-73 0.7793103974606339 1101 74-75 0.6633459296576731 1101 76-77 0.7400577915517488 1101 78-79 0.6648620827612319 1101 80-81 0.7383998732334405 1101 82-83 0.6930135600301881 1101 84-85 0.6579916239410935 1101 86-87 0.6178223729868151 1101 88-89 0.6602707846938216 1101 90-91 0.7078215301931081 1101 92-93 0.6396166516892521 1101 94-95 0.6077997054116757 1101 96-97 0.6214571733701817 1101 98-99 0.7405502642333666 1101 100 0.7461095104213307 1102 1 0.12834712320206165 1102 2 0.1678453726748934 1102 3 0.24593499258909546 1102 4 0.2938128405852609 1102 5 0.27021611667262135 1102 6 0.38344707678958656 1102 7 0.41687244565915194 1102 8 0.45395771783071837 1102 9 0.5486653288742644 1102 10-11 0.4937987740034515 1102 12-13 0.5214910033515139 1102 14-15 0.5598275891241258 1102 16-17 0.5361281103881197 1102 18-19 0.5867062585645186 1102 20-21 0.5115693011635756 1102 22-23 0.524885343240939 1102 24-25 0.5626302545691573 1102 26-27 0.5513476544520302 1102 28-29 0.605160859662746 1102 30-31 0.5701881560860045 1102 32-33 0.6172678563354737 1102 34-35 0.591060505646773 1102 36-37 0.5464137332925034 1102 38-39 0.5450707382980653 1102 40-41 0.6274830862856646 1102 42-43 0.6289279710936242 1102 44-45 0.5157702262053476 1102 46-47 0.449274463133456 1102 48-49 0.4520515801638325 1102 50-51 0.501955343264413 1102 52-53 0.4932878345678162 1102 54-55 0.48770454931422336 1102 56-57 0.43067389503360687 1102 58-59 0.532563777610207 1102 60-61 0.5165706570789084 1102 62-63 0.5435678864662279 1102 64-65 0.41391374802897474 1102 66-67 0.47500074769927636 1102 68-69 0.5120563652147752 1102 70-71 0.519474948172018 1102 72-73 0.4659291693619707 1102 74-75 0.43368733797704095 1102 76-77 0.48201540999239967 1102 78-79 0.4120561947574828 1102 80-81 0.390068222139611 1102 82-83 0.3741727140729658 1102 84-85 0.4660768239928288 1102 86-87 0.5077105058333728 1102 88-89 0.4781572464133532 1102 90-91 0.5081055366704987 1102 92-93 0.5170643219826019 1102 94-95 0.576298629984219 1102 96-97 0.5018624996974523 1102 98-99 0.5210603079006617 1102 100 0.49409078755675395 1103 1 0.08420911131830167 1103 2 0.1164787954549027 1103 3 0.15303704949342745 1103 4 0.17268113243065386 1103 5 0.28576949990778644 1103 6 0.38754297765792955 1103 7 0.29911326491317425 1103 8 0.3552812794146121 1103 9 0.41509037874378407 1103 10-11 0.39589569395467805 1103 12-13 0.455494471371793 1103 14-15 0.49917032682013485 1103 16-17 0.46011085059491563 1103 18-19 0.47025437007655313 1103 20-21 0.45939318074933055 1103 22-23 0.45615226449370994 1103 24-25 0.42765189623413846 1103 26-27 0.36905698875275306 1103 28-29 0.4574941348418946 1103 30-31 0.5418277672098881 1103 32-33 0.5016556035253785 1103 34-35 0.48190885983715503 1103 36-37 0.49822371310469293 1103 38-39 0.5153613107660249 1103 40-41 0.5061905771909991 1103 42-43 0.5831700420472714 1103 44-45 0.473114476833274 1103 46-47 0.43978741536302124 1103 48-49 0.5232882427781718 1103 50-51 0.45698937269412454 1103 52-53 0.49147743936858035 1103 54-55 0.4672479374140224 1103 56-57 0.401429677913562 1103 58-59 0.4604225404768272 1103 60-61 0.4148306930606509 1103 62-63 0.4429530444102241 1103 64-65 0.44492414470514063 1103 66-67 0.49146588241852385 1103 68-69 0.49778816641607904 1103 70-71 0.4015432664175762 1103 72-73 0.42764700709702197 1103 74-75 0.38094511425327227 1103 76-77 0.4917078435789364 1103 78-79 0.3997223380364687 1103 80-81 0.4032857917192487 1103 82-83 0.36283089392078693 1103 84-85 0.5101448893138247 1103 86-87 0.5145837537077469 1103 88-89 0.3759723797941348 1103 90-91 0.4369601207434499 1103 92-93 0.4211416458192687 1103 94-95 0.5044081943170546 1103 96-97 0.41412449445988386 1103 98-99 0.4040059235827229 1103 100 0.3351621353584804 1104 1 -0.04767644422477346 1104 2 0.0061433018738732414 1104 3 0.019518204421000718 1104 4 0.1958813316077439 1104 5 0.17581963113254062 1104 6 0.2202903859222829 1104 7 0.2194255835665615 1104 8 0.24606802889701385 1104 9 0.29867567857642996 1104 10-11 0.31573534142621895 1104 12-13 0.24705911594178076 1104 14-15 0.3967045744212072 1104 16-17 0.4010718800259454 1104 18-19 0.3608300554965638 1104 20-21 0.3355832825467928 1104 22-23 0.3394998247616243 1104 24-25 0.4018103543339464 1104 26-27 0.2696528352366414 1104 28-29 0.3513359313293378 1104 30-31 0.30439192881289046 1104 32-33 0.2800238302877389 1104 34-35 0.3572879327651677 1104 36-37 0.32140069991482534 1104 38-39 0.4358013910358878 1104 40-41 0.366575677846555 1104 42-43 0.3953464260846502 1104 44-45 0.28944896329709024 1104 46-47 0.25032723393216116 1104 48-49 0.17304451292519474 1104 50-51 0.2378009818727449 1104 52-53 0.19048136675131389 1104 54-55 0.23160216562154545 1104 56-57 0.213787642563382 1104 58-59 0.30239967255144506 1104 60-61 0.30429957912912187 1104 62-63 0.26241184058692824 1104 64-65 0.24314970900901756 1104 66-67 0.3226955949970076 1104 68-69 0.3411210691974418 1104 70-71 0.31351551266334354 1104 72-73 0.3629921412981929 1104 74-75 0.3683940564058119 1104 76-77 0.3273497778430112 1104 78-79 0.2336359805435606 1104 80-81 0.30312723627725546 1104 82-83 0.22215739832552117 1104 84-85 0.2597728362548466 1104 86-87 0.2868044471584916 1104 88-89 0.2501225076493476 1104 90-91 0.2493620765725666 1104 92-93 0.2479844082406899 1104 94-95 0.28804927870898567 1104 96-97 0.2941727162802579 1104 98-99 0.318748678141894 1104 100 0.24229394790738468 1105 1 0.05811951480069766 1105 2 0.10984037208676156 1105 3 0.14191985812941255 1105 4 0.2316800719790777 1105 5 0.17055642385546577 1105 6 0.2474524457758207 1105 7 0.17504693636227842 1105 8 0.26245770557326864 1105 9 0.31289726943936813 1105 10-11 0.3017076042450242 1105 12-13 0.20874402424966831 1105 14-15 0.2937091826460758 1105 16-17 0.27463292014243734 1105 18-19 0.2835612115960089 1105 20-21 0.20754858900372142 1105 22-23 0.233649068487928 1105 24-25 0.23680958482681547 1105 26-27 0.1833753823691211 1105 28-29 0.2049460057523902 1105 30-31 0.2678619177522279 1105 32-33 0.2169470935939657 1105 34-35 0.25710508631505746 1105 36-37 0.2327194222992901 1105 38-39 0.24331094015683163 1105 40-41 0.29760851931649057 1105 42-43 0.27964034236514124 1105 44-45 0.22495821651723702 1105 46-47 0.1894861728402475 1105 48-49 0.20293233950473422 1105 50-51 0.2933517519901869 1105 52-53 0.3396207328555292 1105 54-55 0.28099870305228336 1105 56-57 0.2598220115364711 1105 58-59 0.16609132211542033 1105 60-61 0.20580917055883674 1105 62-63 0.18581753712901872 1105 64-65 0.1135420325465617 1105 66-67 0.1147668936231625 1105 68-69 0.04616456405168634 1105 70-71 0.08157033719074036 1105 72-73 0.07120357084437501 1105 74-75 0.11916151108861328 1105 76-77 0.21832861090183542 1105 78-79 0.12648320863898732 1105 80-81 0.06508019282879474 1105 82-83 0.07423292174783569 1105 84-85 0.10809620335223258 1105 86-87 0.09009928862850813 1105 88-89 0.10816337288725464 1105 90-91 0.12237057233966553 1105 92-93 0.1174593828391366 1105 94-95 0.1048386647143964 1105 96-97 0.14983119350577567 1105 98-99 0.1788188551076857 1105 100 0.17244726422251233 1106 1 -0.07017426676740968 1106 2 0.0029744496719850133 1106 3 0.042866815269512415 1106 4 0.05577977438284876 1106 5 0.08200572238630599 1106 6 0.09694532587465687 1106 7 0.09605486444687017 1106 8 0.12260899332477493 1106 9 0.2025533172869345 1106 10-11 0.19224046306771214 1106 12-13 0.20315905707710158 1106 14-15 0.25193507474676125 1106 16-17 0.26630422400334197 1106 18-19 0.2802387655074483 1106 20-21 0.2736847669413862 1106 22-23 0.3045778276927251 1106 24-25 0.2552368577449897 1106 26-27 0.2011001533387713 1106 28-29 0.26707518884995807 1106 30-31 0.28570913295720146 1106 32-33 0.2910875124328314 1106 34-35 0.2713057970085444 1106 36-37 0.22987088055253224 1106 38-39 0.26559410720287957 1106 40-41 0.2864807224330832 1106 42-43 0.22528551559450705 1106 44-45 0.2486856935935009 1106 46-47 0.24281912594617694 1106 48-49 0.26048000400937354 1106 50-51 0.2694248378463868 1106 52-53 0.32209404763750626 1106 54-55 0.34800657626916376 1106 56-57 0.3266654103035904 1106 58-59 0.3526686133203327 1106 60-61 0.3758907984045976 1106 62-63 0.4023345834536016 1106 64-65 0.3560772937944279 1106 66-67 0.36031307974530336 1106 68-69 0.35390659818923353 1106 70-71 0.38104081654732624 1106 72-73 0.3875594716752033 1106 74-75 0.3430868894511647 1106 76-77 0.41502714023900467 1106 78-79 0.2484000982924357 1106 80-81 0.3606029529245589 1106 82-83 0.37935775727606824 1106 84-85 0.3661647443684153 1106 86-87 0.4163795644591133 1106 88-89 0.399837156305594 1106 90-91 0.4158762575079855 1106 92-93 0.3611592531750105 1106 94-95 0.4400792374466924 1106 96-97 0.38991329088018745 1106 98-99 0.4434577943307083 1106 100 0.4569746563932391 1107 1 0.0032449706638146836 1107 2 0.027277934109537938 1107 3 0.05165750264333724 1107 4 0.17253800911011297 1107 5 0.10471330613606256 1107 6 0.1380529755172475 1107 7 0.16930384602554227 1107 8 0.25967463722503226 1107 9 0.24080754452882758 1107 10-11 0.2508731727167941 1107 12-13 0.18323765074456588 1107 14-15 0.21241742879293923 1107 16-17 0.27505164894489553 1107 18-19 0.22538555237024127 1107 20-21 0.24934351185184767 1107 22-23 0.2720614338221594 1107 24-25 0.23002961821788404 1107 26-27 0.2528601292269741 1107 28-29 0.3164270457413778 1107 30-31 0.24182971248994534 1107 32-33 0.31015501624444397 1107 34-35 0.27301275962195604 1107 36-37 0.2438630729684803 1107 38-39 0.3137688569739083 1107 40-41 0.3472273516103357 1107 42-43 0.33746347759846884 1107 44-45 0.21806423051328494 1107 46-47 0.17512607717933548 1107 48-49 0.2564002190841279 1107 50-51 0.30647015896048657 1107 52-53 0.25255457702892414 1107 54-55 0.2335062096954914 1107 56-57 0.1944452334644282 1107 58-59 0.3382460572755974 1107 60-61 0.326597058411366 1107 62-63 0.22960172471814388 1107 64-65 0.33138672948766157 1107 66-67 0.26629571002269614 1107 68-69 0.2978257428893514 1107 70-71 0.256290964473493 1107 72-73 0.29885538338495365 1107 74-75 0.32351032426605997 1107 76-77 0.4065856447822327 1107 78-79 0.24725736011936306 1107 80-81 0.2816621729561106 1107 82-83 0.2477426679880388 1107 84-85 0.24036231529107965 1107 86-87 0.2631621148700276 1107 88-89 0.3029533438643668 1107 90-91 0.39561094842931155 1107 92-93 0.3576559345614996 1107 94-95 0.31626726414574335 1107 96-97 0.2687012543309919 1107 98-99 0.2339226497520741 1107 100 0.18451841489856946 1108 1 -0.1505648049006858 1108 2 -0.07304149422800066 1108 3 0.004851615475047311 1108 4 -0.04514268193293702 1108 5 0.0544881458249904 1108 6 0.10592569875893787 1108 7 0.08259896335960804 1108 8 -0.0039783660494094875 1108 9 0.15874234567488088 1108 10-11 0.08531351226929473 1108 12-13 0.1711495137638721 1108 14-15 0.19692040051680237 1108 16-17 0.1738527054630694 1108 18-19 0.15397534788836253 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2214 72-73 -0.19590662448617024 2214 74-75 -0.0904286993633363 2214 76-77 0.004353386344256194 2214 78-79 0.06322610465608136 2214 80-81 0.08672205174971737 2214 82-83 0.1002887146756386 2214 84-85 0.14501304450125474 2214 86-87 0.09325193535417498 2214 88-89 0.13997506502926527 2214 90-91 0.07642366504208553 2214 92-93 0.10833143152808589 2214 94-95 0.11174204856724046 2214 96-97 0.07356828144620664 2214 98-99 0.11154568071944482 2214 100 0.13186637224929143 2215 1 -0.17082181784107675 2215 2 -0.19234960205890417 2215 3 -0.18151541644002833 2215 4 -0.1944260030153444 2215 5 -0.21110361642735143 2215 6 -0.25398818865794226 2215 7 -0.3574192848155775 2215 8 -0.2473241070022496 2215 9 -0.3690910360923283 2215 10-11 -0.4845382159653795 2215 12-13 -0.47353490391848396 2215 14-15 -0.5296438783597566 2215 16-17 -0.47250366387658005 2215 18-19 -0.46939208146940814 2215 20-21 -0.4387224750377783 2215 22-23 -0.43999418952263625 2215 24-25 -0.49589199995060085 2215 26-27 -0.4485316855424486 2215 28-29 -0.4616166641778534 2215 30-31 -0.49205812840173024 2215 32-33 -0.44678046056616694 2215 34-35 -0.4358574347902575 2215 36-37 -0.5019467431251101 2215 38-39 -0.30781196663789956 2215 40-41 -0.44015770688295675 2215 42-43 -0.35674321055404334 2215 44-45 -0.24005308164062455 2215 46-47 -0.34515950851962174 2215 48-49 -0.2720762741637017 2215 50-51 -0.2734316294896928 2215 52-53 -0.301501616748574 2215 54-55 -0.36115945791596715 2215 56-57 -0.3193157716462096 2215 58-59 -0.20478382272834494 2215 60-61 -0.1059662021655754 2215 62-63 -0.16710053813505255 2215 64-65 -0.12171621241301267 2215 66-67 -0.07550890241136443 2215 68-69 -0.11094623313332619 2215 70-71 -0.09293388997725316 2215 72-73 -0.17008339290693186 2215 74-75 -0.14316826631372948 2215 76-77 -0.08751817397064343 2215 78-79 -0.23002758538174106 2215 80-81 -0.26237759181221065 2215 82-83 -0.19455881595776958 2215 84-85 -0.11986782079200253 2215 86-87 -0.018779038908782297 2215 88-89 -0.03943315780999512 2215 90-91 -0.10225129685948886 2215 92-93 -0.05982129600822361 2215 94-95 -0.08568687826271315 2215 96-97 -0.011594262642461217 2215 98-99 -0.15974038319896522 2215 100 -0.07087880872199648 2216 1 -0.6859055177662512 2216 2 -0.6621144204258016 2216 3 -0.8032751345266718 2216 4 -0.9802787033606393 2216 5 -0.9095490407799431 2216 6 -2.3780772732341546 2216 7 -2.1613974500684847 2216 8 -3.054092177791077 2216 9 -2.6273461582614033 2216 10-11 -1.66623875788855 2216 12-13 -1.4556720359378943 2216 14-15 -1.6570816083303797 2216 16-17 -1.9935195343786063 2216 18-19 -1.6029939529823736 2216 20-21 -1.410596093190712 2216 22-23 -1.6086550385441853 2216 24-25 -1.7744012523018995 2216 26-27 -1.7458937923805138 2216 28-29 -2.2027628872334475 2216 30-31 -1.8572540269508835 2216 32-33 -1.9353846828286052 2216 34-35 -2.955531097235884 2216 36-37 -1.9030563410255539 2216 38-39 -1.7730062971010412 2216 40-41 -2.374859662014913 2216 42-43 -2.1193803514025618 2216 44-45 -1.8112153773479918 2216 46-47 -1.5905423229222535 2216 48-49 -1.8006487098050385 2216 50-51 -2.0588250323816695 2216 52-53 -2.39935933533701 2216 54-55 -1.778778932275138 2216 56-57 -1.7670427504879953 2216 58-59 -2.86995402490016 2216 60-61 -3.430179160938806 2216 62-63 -2.518229256568244 2216 64-65 -2.5314575030517332 2216 66-67 -2.2125449282909457 2216 68-69 -2.5358897728396563 2216 70-71 -2.319628246008012 2216 72-73 -2.824688098195054 2216 74-75 -3.2712685701085533 2216 76-77 -4.009361708784176 2216 78-79 -3.052094473585395 2216 80-81 -2.900222078697677 2216 82-83 -3.1165009884402437 2216 84-85 -3.146227415749408 2216 86-87 -2.805405136752931 2216 88-89 -2.659866394685345 2216 90-91 -2.8843659054615394 2216 92-93 -2.942950212544474 2216 94-95 -2.758903936868112 2216 96-97 -2.9936285493720938 2216 98-99 -2.8395616361079696 2216 100 -2.4733828075712694 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 84312.0 3 14790.0 4 9618.0 5 7596.0 6 6990.0 7 6950.0 8 7823.0 9 8975.0 10 10196.0 11 11379.0 12 12357.0 13 13908.0 14 15609.0 15 16774.0 16 16979.0 17 17796.0 18 16586.0 19 15196.0 20 15049.0 21 15917.0 22 17422.0 23 20764.0 24 25304.0 25 30876.0 26 38113.0 27 44725.0 28 54220.0 29 66849.0 30 80346.0 31 96233.0 32 120234.0 33 158659.0 34 216106.0 35 309074.0 36 497375.0 37 842251.0 38 1043626.0 39 341185.0 40 610.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.22570995727306 20.336062500129962 14.35454554486259 23.083681997734384 2 15.175235480620799 20.068194633167874 40.87095477108234 23.885615115128996 3 18.130259373453125 25.348984141055087 30.339654942118294 26.181101543373497 4 12.462042113495121 16.805787809290333 39.31718326368032 31.414986813534224 5 13.002769565836353 37.98608376365139 35.78644669140418 13.224699979108081 6 30.79003897561049 38.10668432297643 17.692246360986026 13.411030340427057 7 26.750100816585455 32.68310776224156 22.72975493192425 17.837036489248735 8 24.590784865730292 36.68048935266476 20.6501407612245 18.07858502038045 9 24.634530527314134 17.083251101065038 21.80808999129686 36.474128380323975 10-11 23.64210518774662 27.219576587214423 29.155678387279753 19.98263983775921 12-13 23.999135216682877 24.66131149636055 29.2408498957326 22.098703391223975 14-15 21.43756775513194 26.939521344585355 26.38754306227891 25.235367838003793 16-17 20.997734852490417 30.29923656995382 27.026026780597284 21.677001796958482 18-19 20.95285690103121 29.299633307700883 28.996400094980647 20.751109696287262 20-21 22.04452517381272 28.440821637011332 28.59311358460349 20.921539604572455 22-23 21.6916644943492 28.286797209080472 28.64394699915141 21.377591297418917 24-25 21.517745445276592 28.47810068810909 28.571542466440054 21.432611400174267 26-27 21.29303619264537 28.431537258439928 28.64483482495272 21.630591723961984 28-29 21.325145871516597 28.42059433818158 28.79265365414244 21.461606136159382 30-31 21.551508683717234 28.770467169162607 28.380948661458373 21.297075485661786 32-33 21.295958323901882 28.550267125292315 28.729837346885507 21.423937203920296 34-35 21.497785211759084 28.277454454026042 28.744064395245417 21.480695938969458 36-37 21.677128751584103 28.627293886451177 28.273880775676325 21.421696586288398 38-39 21.53865974021172 28.732047409858648 28.384365099355957 21.344927750573675 40-41 21.499507893235364 28.457232005172724 28.51369891662563 21.529561184966283 42-43 21.416292301008475 28.48417727369365 28.485945111523993 21.613585313773882 44-45 21.5312007638834 28.56701149568721 28.387733681628667 21.51405405880072 46-47 21.537915999600685 28.663590306252612 28.22453995555769 21.57395373858901 48-49 21.4832331114448 28.52374567811881 28.255801754310557 21.73721945612583 50-51 21.551712483920603 28.47744505332299 28.23154423794029 21.73929822481611 52-53 21.499131220084056 28.477771412553643 28.32509076606769 21.69800660129461 54-55 21.61960741222687 28.76021392702508 28.088892925218783 21.531285735529266 56-57 21.521114047677752 28.549551500233278 28.127326006885845 21.802008445203118 58-59 21.465975906109065 28.20689956380315 28.43483067446441 21.89229385562337 60-61 21.638400225538412 28.612112637488018 28.08373806809943 21.665749068874142 62-63 21.518843133031936 28.598698251980633 28.11506045645758 21.767398158529854 64-65 21.57656256083906 28.394338675867044 28.17415398970792 21.85494477358597 66-67 21.4576258144038 28.42295698727815 28.101799767555008 22.01761743076304 68-69 21.74039938360836 28.470643233123667 28.07238268968734 21.716574693580633 70-71 21.746795962417544 28.351952902805305 28.13400405301901 21.767247081758146 72-73 21.603849340091667 28.475330416326305 28.21750227325508 21.703317970326943 74-75 21.6982512021411 28.46293934562946 28.1043247501379 21.734484702091535 76-77 21.68944118611828 28.463021210707755 27.949636975311503 21.89790062786246 78-79 21.728130371601445 28.48342053065717 28.08827979462711 21.700169303114276 80-81 21.69554109821628 28.37213741273863 28.129430660520317 21.80289082852478 82-83 21.605363203780115 28.4301192747386 28.22720710028543 21.73731042119585 84-85 21.622575306404183 28.371301000542122 28.08556717182898 21.92055652122472 86-87 21.770982791438538 28.514752027700446 28.000683545163778 21.71358163569723 88-89 21.60381095081046 28.396974736009227 28.05669146355892 21.94252284962139 90-91 21.58852182585762 28.34552653051604 28.100634258851652 21.96531738477469 92-93 21.668280396076213 28.51796092386972 27.948179643901145 21.86557903615293 94-95 21.586210060023127 28.34267936600957 28.069005622409033 22.00210495155827 96-97 21.594576937755093 28.383904257373683 28.053926148108516 21.9675926567627 98-99 21.641414701444198 28.406555022994972 27.96329998438356 21.988730291177266 100 21.654593958748578 28.30088995216195 27.968601718917053 22.07591437017242 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1482.0 1 1191.5 2 1073.0 3 1278.0 4 1199.0 5 942.5 6 754.0 7 709.0 8 712.0 9 719.0 10 680.0 11 637.0 12 671.0 13 724.0 14 765.5 15 933.0 16 1159.0 17 1447.0 18 1895.5 19 2528.5 20 3609.0 21 5115.5 22 7422.0 23 10930.5 24 15704.0 25 22272.5 26 31118.0 27 41586.5 28 54131.0 29 68962.0 30 83792.5 31 96482.0 32 106159.0 33 115211.5 34 124201.0 35 131453.5 36 137938.5 37 145179.0 38 151633.0 39 154934.0 40 157352.0 41 159372.5 42 158598.0 43 156431.0 44 153419.5 45 149767.0 46 145958.5 47 140521.5 48 134989.0 49 130879.5 50 127440.0 51 122886.0 52 115618.0 53 108598.5 54 103182.0 55 98227.5 56 91219.5 57 82440.5 58 74756.0 59 67555.5 60 59361.5 61 50541.5 62 43162.0 63 36184.5 64 29247.5 65 23535.5 66 18980.0 67 14881.5 68 11337.5 69 8722.0 70 6575.5 71 4909.0 72 3698.0 73 2735.0 74 2001.0 75 1422.0 76 1043.5 77 769.5 78 517.0 79 333.0 80 224.5 81 156.5 82 112.5 83 88.0 84 70.5 85 54.5 86 48.0 87 33.0 88 23.0 89 20.0 90 16.0 91 15.5 92 14.5 93 13.5 94 11.0 95 9.5 96 10.0 97 8.5 98 11.0 99 14.0 100 31.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013606630240631756 2 0.03354300018573397 3 0.03460565721641149 4 0.03714679359411861 5 0.04019615724736715 6 0.039433816334055016 7 0.037123692354321274 8 0.03663856631857719 9 0.036915781196145234 10-11 0.03750486281097734 12-13 0.03872922852023623 14-15 0.037169894833915945 16-17 0.03372781010411267 18-19 0.036372902060907805 20-21 0.0359686303644544 22-23 0.03686957871655056 24-25 0.034155183040363406 26-27 0.03428223985924877 28-29 0.03476736589499285 30-31 0.03480201775468886 32-33 0.034547904116918146 34-35 0.03455945473681682 36-37 0.03234173571627242 38-39 0.0323879381958671 40-41 0.03370470886431533 42-43 0.03354300018573397 44-45 0.03257274811424579 46-47 0.031510091083568274 48-49 0.031741103481541647 50-51 0.032746007412725826 52-53 0.03324268406836858 54-55 0.03253809625454979 56-57 0.03107116752741886 58-59 0.03160249604275762 60-61 0.03117512310650688 62-63 0.031417686124378924 64-65 0.029303922682922548 66-67 0.029950757397247996 68-69 0.030689997070762795 70-71 0.03189126154022434 72-73 0.029500283221199918 74-75 0.029685093139578615 76-77 0.030297275994208055 78-79 0.03064379459116812 80-81 0.030666895830965456 82-83 0.029661991899781276 84-85 0.03044743405289075 86-87 0.03079395264985081 88-89 0.031140471246810873 90-91 0.031394584884581585 92-93 0.030539839012080103 94-95 0.03133683178508824 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4328772.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.89165815298065 #Duplication Level Percentage of deduplicated Percentage of total 1 78.63369682356561 51.0267097357994 2 12.939198998499075 16.79292156367983 3 3.9193447080844073 7.629983309421212 4 1.6244931251430828 4.216642101946086 5 0.8049958545968082 2.61187579055313 6 0.4910055145632166 1.911729720135877 7 0.32761712856047365 1.4881733098125158 8 0.22461207958043772 1.166036022813108 9 0.15660091373472412 0.9145883664466318 >10 0.828187480415434 9.386043794145998 >50 0.0400083905256357 1.739578150403147 >100 0.010060840613486733 1.0062686632766311 >500 1.0688529532467425E-4 0.04697959161158153 >1k 7.125682223800233E-5 0.06246987995491142 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.3101239797337445E-5 0.0 3 0.0 0.0 0.0 6.930371939201233E-5 0.0 4 0.0 0.0 0.0 9.240495918934978E-5 0.0 5 0.0 0.0 0.0 9.240495918934978E-5 0.0 6 0.0 0.0 0.0 9.240495918934978E-5 0.0 7 0.0 0.0 0.0 9.240495918934978E-5 0.0 8 0.0 0.0 0.0 1.8480991837869956E-4 0.0 9 0.0 0.0 0.0 2.3101239797337445E-4 0.0 10-11 0.0 0.0 0.0 2.3101239797337445E-4 0.0 12-13 0.0 0.0 0.0 2.656642576693806E-4 0.0 14-15 0.0 0.0 0.0 2.8876549746671806E-4 0.0 16-17 0.0 0.0 0.0 3.4651859696006166E-4 0.0 18-19 0.0 0.0 0.0 3.580692168587304E-4 0.0 20-21 0.0 0.0 0.0 3.9272107655473657E-4 0.0 22-23 0.0 0.0 0.0 5.544297551360986E-4 0.0 24-25 0.0 0.0 0.0 7.738915332108044E-4 0.0 26-27 0.0 0.0 0.0 0.0010511064107788536 0.0 28-29 0.0 0.0 0.0 0.0022639215001390694 0.0 30-31 1.1550619898668722E-5 0.0 0.0 0.006040974207003742 0.0 32-33 3.465185969600617E-5 0.0 0.0 0.0153623244652294 0.0 34-35 4.620247959467489E-5 0.0 0.0 0.03007781421613335 0.0 36-37 4.620247959467489E-5 0.0 0.0 0.05382588872779624 0.0 38-39 4.620247959467489E-5 0.0 0.0 0.0856016440690339 0.0 40-41 4.620247959467489E-5 0.0 0.0 0.11726189321128486 0.0 42-43 5.775309949334361E-5 0.0 0.0 0.1506778365781335 0.0 44-45 6.930371939201233E-5 0.0 0.0 0.1927682954888823 0.0 46-47 6.930371939201233E-5 0.0 0.0 0.2408997286066349 0.0 48-49 6.930371939201233E-5 0.0 0.0 0.290232426193849 0.0 50-51 6.930371939201233E-5 0.0 0.0 0.33951892130146843 0.0 52-53 6.930371939201233E-5 0.0 0.0 0.389429149883616 0.0 54-55 6.930371939201233E-5 0.0 0.0 0.4428507669149588 0.0 56-57 6.930371939201233E-5 0.0 0.0 0.5073494284291249 0.0 58-59 9.240495918934978E-5 0.0 0.0 0.5770227676578947 0.0 60-61 1.1550619898668722E-4 0.0 0.0 0.6459915190728456 0.0 62-63 1.1550619898668722E-4 0.0 0.0 0.7166004585134075 0.0 64-65 1.1550619898668722E-4 0.0 0.0 0.7916448359950582 0.0 66-67 1.1550619898668722E-4 0.0 0.0 0.8799146732606846 0.0 68-69 1.1550619898668722E-4 0.0 0.0 0.9762814950752776 0.0 70-71 1.2705681888535594E-4 0.0 0.0 1.0758709398415993 0.0 72-73 1.3860743878402467E-4 0.0 0.0 1.1766038959778893 0.0 74-75 1.3860743878402467E-4 0.0 0.0 1.280062798410265 0.0 76-77 1.3860743878402467E-4 0.0 0.0 1.3899669467460978 0.0 78-79 1.3860743878402467E-4 0.0 0.0 1.5150948121083763 0.0 80-81 1.3860743878402467E-4 0.0 0.0 1.6520505122468911 0.0 82-83 1.3860743878402467E-4 0.0 0.0 1.7903691855334491 0.0 84-85 1.3860743878402467E-4 0.0 0.0 1.9299006739093674 0.0 86-87 1.3860743878402467E-4 1.1550619898668722E-5 0.0 2.081225345201826 0.0 88 1.3860743878402467E-4 2.3101239797337445E-5 0.0 2.201109229130109 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4200 0.0 43.537697 1 GTATCAA 7350 0.0 37.925602 1 TCAACGC 7655 0.0 34.26487 4 ATCAACG 7835 0.0 33.53767 3 CAACGCA 8145 0.0 32.43436 5 AACGCAG 8670 0.0 30.576302 6 TATCAAC 9030 0.0 29.411745 2 ACGCAGA 9980 0.0 26.186005 7 CGCAGAG 10420 0.0 24.809612 8 GTACATG 11465 0.0 24.72341 1 TACATGG 11745 0.0 23.333273 2 ACATGGG 12175 0.0 21.698387 3 GCAGAGT 12140 0.0 20.32664 9 AGAGTAC 10295 0.0 18.239605 10-11 GAGTACA 8660 0.0 18.23839 1 CATGGGG 9190 0.0 17.851307 4 GTGGTAT 2745 0.0 17.467186 1 CAGAGTA 11485 0.0 17.311485 10-11 GTCGTAC 285 9.585401E-7 16.493782 1 AGTACAT 9270 0.0 16.125288 2 >>END_MODULE