FastQCFastQC Report
Wed 25 May 2016
SRR2049362_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049362_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4328772
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111350.25723230514335244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102230.23616397444818069No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61800.1427656619475454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70700.054.597681
GTATCAA132750.043.1737331
ATCAACG163900.034.493833
TCAACGC164700.034.297754
TATCAAC170550.033.2590832
CAACGCA171100.033.097245
AACGCAG177400.031.9749436
GTGGTAT32650.028.5123121
ACGCAGA203800.027.6486347
CGCAGAG208200.026.996558
TGGTATC31700.026.8332962
GTACATG115700.024.3006691
GCAGAGT229250.024.168919
TACATGG118600.022.665542
GAGTACT164450.021.29929712-13
AGAGTAC210100.021.06612610-11
ACATGGG122800.020.8187793
CAGAGTA222550.020.66961110-11
AGTACTT171050.019.6391212-13
GTACTTT179350.019.52156614-15
Produced by FastQC (version 0.11.5)