##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049362_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4328772 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.786726813054603 31.0 31.0 34.0 30.0 34.0 2 32.052941573268356 33.0 31.0 34.0 30.0 34.0 3 32.01483538518545 34.0 31.0 34.0 30.0 34.0 4 35.71887431354666 37.0 35.0 37.0 33.0 37.0 5 35.81149550034051 37.0 35.0 37.0 35.0 37.0 6 35.865322775142694 37.0 35.0 37.0 35.0 37.0 7 35.81606446354763 37.0 35.0 37.0 35.0 37.0 8 35.804135907365875 37.0 35.0 37.0 35.0 37.0 9 37.5347438026304 39.0 37.0 39.0 35.0 39.0 10-11 37.495600946411585 39.0 37.0 39.0 35.0 39.0 12-13 37.443478196587854 39.0 37.0 39.0 34.5 39.0 14-15 38.74151052538687 40.0 38.0 41.0 34.5 41.0 16-17 38.7693665778655 40.0 38.0 41.0 35.0 41.0 18-19 38.841323474648235 40.0 38.0 41.0 35.0 41.0 20-21 38.871697793277164 40.0 38.0 41.0 35.0 41.0 22-23 38.8422624245398 40.0 38.0 41.0 35.0 41.0 24-25 38.82420291482203 40.0 38.0 41.0 35.0 41.0 26-27 38.71239637938889 40.0 38.0 41.0 34.5 41.0 28-29 38.63203444302449 40.0 38.0 41.0 34.0 41.0 30-31 38.528928527536216 40.0 38.0 41.0 34.0 41.0 32-33 38.39494988417039 40.0 38.0 41.0 34.0 41.0 34-35 38.29258413240522 40.0 38.0 41.0 34.0 41.0 36-37 38.1822102203581 40.0 38.0 41.0 34.0 41.0 38-39 38.08706556963499 40.0 38.0 41.0 33.5 41.0 40-41 37.97488745075971 40.0 38.0 41.0 33.0 41.0 42-43 37.839071219274196 40.0 37.5 41.0 33.0 41.0 44-45 37.66090510195501 40.0 37.0 41.0 33.0 41.0 46-47 37.43603994389171 40.0 37.0 41.0 32.5 41.0 48-49 37.227964189382114 40.0 37.0 41.0 32.0 41.0 50-51 37.088420572855306 40.0 37.0 41.0 31.5 41.0 52-53 36.95256703286752 40.0 36.5 41.0 31.0 41.0 54-55 36.818036962907726 40.0 36.0 41.0 31.0 41.0 56-57 36.676950183562454 39.0 36.0 41.0 31.0 41.0 58-59 36.49166149198895 39.0 35.0 41.0 31.0 41.0 60-61 36.31836996727941 39.0 35.0 41.0 31.0 41.0 62-63 36.12051038955158 39.0 35.0 41.0 30.0 41.0 64-65 35.894922393695026 38.5 35.0 40.5 30.0 41.0 66-67 35.63218021184761 38.0 35.0 40.0 30.0 41.0 68-69 35.349652973175765 37.0 35.0 40.0 29.0 41.0 70-71 35.02133769115121 37.0 34.5 39.5 29.0 41.0 72-73 34.72905837960512 36.0 34.0 39.0 29.0 41.0 74-75 34.424062413081586 36.0 34.0 39.0 29.0 41.0 76-77 34.09734157862784 35.5 34.0 38.5 29.0 40.0 78-79 33.764126292629875 35.0 34.0 37.0 29.0 39.0 80-81 33.445630539099774 35.0 34.0 37.0 28.5 39.0 82-83 33.14471298095626 35.0 34.0 36.5 28.0 39.0 84-85 32.87176882958954 35.0 34.0 36.0 28.0 37.5 86-87 32.63655350755364 35.0 33.5 36.0 27.5 37.0 88-89 32.40791903569881 35.0 33.0 35.5 27.0 37.0 90-91 32.213842748012596 35.0 33.0 35.0 27.0 36.0 92-93 32.0322932231127 35.0 33.0 35.0 26.0 36.0 94-95 31.87464193078314 35.0 33.0 35.0 26.0 36.0 96-97 31.682336584139797 35.0 33.0 35.0 26.0 36.0 98-99 31.53571128255311 35.0 33.0 35.0 25.5 35.5 100 31.42643710502655 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23625722380051783 1101 2 0.24362038978575384 1101 3 0.4196861110027683 1101 4 0.23014301039352603 1101 5 0.20948921568430023 1101 6 0.1915493710098417 1101 7 0.142463779131333 1101 8 0.15074817463489865 1101 9 0.21813692183240363 1101 10-11 0.269370324588337 1101 12-13 0.19321551324581776 1101 14-15 0.3259147637771136 1101 16-17 0.24457469092461537 1101 18-19 0.29146675823578505 1101 20-21 0.33089845959285213 1101 22-23 0.31131557517556274 1101 24-25 0.280191727189127 1101 26-27 0.3639004301819284 1101 28-29 0.42363801218223784 1101 30-31 0.41128188248082864 1101 32-33 0.44414274142790333 1101 34-35 0.4156319333030325 1101 36-37 0.4132677940615537 1101 38-39 0.4151662397585554 1101 40-41 0.40987403155424573 1101 42-43 0.4802213065979757 1101 44-45 0.48040337930095234 1101 46-47 0.5384836112390943 1101 48-49 0.5844822118501085 1101 50-51 0.4572526669210788 1101 52-53 0.5521119381737734 1101 54-55 0.5284075571741695 1101 56-57 0.5562030921139325 1101 58-59 0.611558748601503 1101 60-61 0.5486493164430684 1101 62-63 0.5561431419859346 1101 64-65 0.5150865429491418 1101 66-67 0.4308797189032063 1101 68-69 0.5334518038251943 1101 70-71 0.4937770062337563 1101 72-73 0.482557789461481 1101 74-75 0.5337193590856728 1101 76-77 0.45273187072951515 1101 78-79 0.4950387861985206 1101 80-81 0.5896017932439293 1101 82-83 0.5125639817840266 1101 84-85 0.5529360532571985 1101 86-87 0.48563815276534683 1101 88-89 0.5485185390058973 1101 90-91 0.6145553811804234 1101 92-93 0.5387467312340846 1101 94-95 0.6270717102126184 1101 96-97 0.6192312915533229 1101 98-99 0.6098434348706547 1101 100 0.6102697636105461 1102 1 0.25276520669487823 1102 2 0.2196430743657274 1102 3 0.38580417607978745 1102 4 0.2168103343512371 1102 5 0.16875190063311152 1102 6 0.20106985354659201 1102 7 0.16912955680002995 1102 8 0.1831164486618988 1102 9 0.19083714202490398 1102 10-11 0.23827602483855514 1102 12-13 0.21224371079534876 1102 14-15 0.2753922173937653 1102 16-17 0.22265459008955446 1102 18-19 0.20456450001544368 1102 20-21 0.21749648426774826 1102 22-23 0.22227839780084224 1102 24-25 0.2629349053710399 1102 26-27 0.3559626595665719 1102 28-29 0.27339028675208965 1102 30-31 0.26457359841477057 1102 32-33 0.29625928953392133 1102 34-35 0.28265694180880274 1102 36-37 0.3141212557510613 1102 38-39 0.34331855915660725 1102 40-41 0.33856767032458635 1102 42-43 0.3515671052905276 1102 44-45 0.3721950672172696 1102 46-47 0.34921399399764397 1102 48-49 0.339047216397951 1102 50-51 0.40200922215800006 1102 52-53 0.3949316718479565 1102 54-55 0.2436714039676673 1102 56-57 0.33459012116075826 1102 58-59 0.34585611274618344 1102 60-61 0.3439393886765174 1102 62-63 0.37552988234485696 1102 64-65 0.338298752674163 1102 66-67 0.2846559623713816 1102 68-69 0.33769877880864385 1102 70-71 0.286631956994853 1102 72-73 0.29083242173906854 1102 74-75 0.31235300231518437 1102 76-77 0.2959684404173828 1102 78-79 0.36662904750954084 1102 80-81 0.3490627294675335 1102 82-83 0.33037972560696716 1102 84-85 0.32583221947712104 1102 86-87 0.3598700620705344 1102 88-89 0.2802733689720256 1102 90-91 0.34281263071056856 1102 92-93 0.3587993688981328 1102 94-95 0.34573515851749903 1102 96-97 0.3985513547005475 1102 98-99 0.401156411569211 1102 100 0.3968075444872028 1103 1 0.16699733605193856 1103 2 0.15806951439722638 1103 3 0.33202078473073016 1103 4 0.17044030941878674 1103 5 0.20164653909407804 1103 6 0.1232109522176259 1103 7 0.11871190820454558 1103 8 0.13267923072123722 1103 9 0.17552048325059388 1103 10-11 0.1600970677923499 1103 12-13 0.17622672331142297 1103 14-15 0.21451683073765793 1103 16-17 0.1528153817987956 1103 18-19 0.20174173735929912 1103 20-21 0.21787183261594123 1103 22-23 0.23638514950185652 1103 24-25 0.20197274897689255 1103 26-27 0.2749159626781932 1103 28-29 0.2585757847596213 1103 30-31 0.27347065812065807 1103 32-33 0.26865497430869567 1103 34-35 0.2660719448503599 1103 36-37 0.1882405659870301 1103 38-39 0.18498298734864704 1103 40-41 0.18678909838831714 1103 42-43 0.24961012461895393 1103 44-45 0.23374864245355553 1103 46-47 0.17677598589852295 1103 48-49 0.17488734130945005 1103 50-51 0.23848848926756006 1103 52-53 0.21785012121735292 1103 54-55 0.16313009464924022 1103 56-57 0.14904545182269402 1103 58-59 0.21408818856448164 1103 60-61 0.20201467434741716 1103 62-63 0.13763594542646018 1103 64-65 0.18600685465998623 1103 66-67 0.21508354794803353 1103 68-69 0.22561549278329096 1103 70-71 0.2843177665426566 1103 72-73 0.28900746491623863 1103 74-75 0.23699438483404123 1103 76-77 0.16023705460312243 1103 78-79 0.17292504585537927 1103 80-81 0.2140502170867009 1103 82-83 0.16823161612571624 1103 84-85 0.14448757469303786 1103 86-87 0.16868563668465697 1103 88-89 0.18581165519585596 1103 90-91 0.2113847239012614 1103 92-93 0.23982135025741513 1103 94-95 0.18246908096196535 1103 96-97 0.1614073067814985 1103 98-99 0.21906905475081828 1103 100 0.2548259913642781 1104 1 0.147900484322804 1104 2 0.16025222567656527 1104 3 0.2807270696662769 1104 4 0.2071907278029812 1104 5 0.15337323968075367 1104 6 0.1350239878336339 1104 7 0.16397227982705687 1104 8 0.14360776283854193 1104 9 0.15150972319533196 1104 10-11 0.19941629025293395 1104 12-13 0.17917179236821568 1104 14-15 0.2343222212482985 1104 16-17 0.23282784908621323 1104 18-19 0.24384925180150674 1104 20-21 0.22759435123263216 1104 22-23 0.23196777991230277 1104 24-25 0.20845536403722065 1104 26-27 0.2517864301827544 1104 28-29 0.29974497670717426 1104 30-31 0.2553523761353276 1104 32-33 0.2948428297480632 1104 34-35 0.2913299016379156 1104 36-37 0.31953738851350977 1104 38-39 0.28044103848324653 1104 40-41 0.2258958528127053 1104 42-43 0.28883715279951616 1104 44-45 0.26193201266460164 1104 46-47 0.2936324698239545 1104 48-49 0.22528766104108655 1104 50-51 0.22785806758871274 1104 52-53 0.1833577607068264 1104 54-55 0.1636968320761838 1104 56-57 0.19368245907468662 1104 58-59 0.2784894030954135 1104 60-61 0.21543900390312842 1104 62-63 0.2222913540951552 1104 64-65 0.21520061802113588 1104 66-67 0.2235593378236942 1104 68-69 0.19724679802965284 1104 70-71 0.22300389041433277 1104 72-73 0.2212306293608819 1104 74-75 0.1892503835461241 1104 76-77 0.18831980669129678 1104 78-79 0.1655059802654648 1104 80-81 0.17421105632608658 1104 82-83 0.17875627177773623 1104 84-85 0.2295038046302551 1104 86-87 0.20409198414383667 1104 88-89 0.15086440717171 1104 90-91 0.1543927471435822 1104 92-93 0.2429119645989104 1104 94-95 0.23569372800295696 1104 96-97 0.20672383735630362 1104 98-99 0.1934474687471237 1104 100 0.18777875419753443 1105 1 0.16186524702074934 1105 2 0.15850590275001508 1105 3 0.23193881861330112 1105 4 0.17101563914894058 1105 5 0.13321428118205603 1105 6 0.11551245350156591 1105 7 0.11137656480245539 1105 8 0.11606079738331232 1105 9 0.07520226367611826 1105 10-11 0.14167391255450923 1105 12-13 0.080219592639871 1105 14-15 0.18751342576651808 1105 16-17 0.22254529640407128 1105 18-19 0.14354899198479387 1105 20-21 0.1520371123032973 1105 22-23 0.1932267552693574 1105 24-25 0.17465133689340462 1105 26-27 0.19485732357424013 1105 28-29 0.16882388209489108 1105 30-31 0.18845335219528891 1105 32-33 0.2530363301906391 1105 34-35 0.2442135204925009 1105 36-37 0.28693048468574034 1105 38-39 0.2745770704909205 1105 40-41 0.24928236488815259 1105 42-43 0.3098691858851552 1105 44-45 0.23650367866905952 1105 46-47 0.33229989935171744 1105 48-49 0.2802836823221426 1105 50-51 0.30234509866771475 1105 52-53 0.31947441850994096 1105 54-55 0.2770432676639629 1105 56-57 0.2589146627941332 1105 58-59 0.25823286075355867 1105 60-61 0.22931354552729744 1105 62-63 0.2931800759787677 1105 64-65 0.27761544391944426 1105 66-67 0.30684783210868716 1105 68-69 0.27151012254634566 1105 70-71 0.3121467528566484 1105 72-73 0.3138204407041414 1105 74-75 0.2888647221203158 1105 76-77 0.28524309649480983 1105 78-79 0.3138228120905566 1105 80-81 0.2753837391572205 1105 82-83 0.26715299681792004 1105 84-85 0.2398791738166679 1105 86-87 0.19572998487785753 1105 88-89 0.22534942923547874 1105 90-91 0.23502325996285833 1105 92-93 0.25035710360516816 1105 94-95 0.24079358605136747 1105 96-97 0.3424340007884972 1105 98-99 0.3475972428534533 1105 100 0.32334221607873914 1106 1 0.1731058573324944 1106 2 0.17501964800290182 1106 3 0.3025072805346056 1106 4 0.19706399056177304 1106 5 0.15295591258455943 1106 6 0.14309525629504805 1106 7 0.20655791600810858 1106 8 0.1319821729336681 1106 9 0.168463609479943 1106 10-11 0.15646607062380724 1106 12-13 0.12266134711722287 1106 14-15 0.2100507045504756 1106 16-17 0.24852448403714078 1106 18-19 0.23995942935149372 1106 20-21 0.22451524098730857 1106 22-23 0.17720719482152703 1106 24-25 0.17398433379913314 1106 26-27 0.21131343413144066 1106 28-29 0.2657199888315205 1106 30-31 0.28026214065200605 1106 32-33 0.2267600890032213 1106 34-35 0.25043666268677356 1106 36-37 0.20779025063396261 1106 38-39 0.24652694833947209 1106 40-41 0.23358611213219405 1106 42-43 0.24596111337491777 1106 44-45 0.23729152801397646 1106 46-47 0.2960325109994244 1106 48-49 0.2471757134471062 1106 50-51 0.17688835121174407 1106 52-53 0.2198889250105509 1106 54-55 0.230910458149296 1106 56-57 0.19097469262217004 1106 58-59 0.22274740169557106 1106 60-61 0.24246465913063986 1106 62-63 0.2635795440493496 1106 64-65 0.22794114644337782 1106 66-67 0.21482112787573016 1106 68-69 0.2672013767204362 1106 70-71 0.2604461893600529 1106 72-73 0.2861099278332091 1106 74-75 0.23143780734375952 1106 76-77 0.2100525979529806 1106 78-79 0.22486600801872925 1106 80-81 0.21782918937631734 1106 82-83 0.2375533550410367 1106 84-85 0.17941322465012632 1106 86-87 0.17181310908075176 1106 88-89 0.1212438054941174 1106 90-91 0.14331977762392967 1106 92-93 0.21483315349344423 1106 94-95 0.252177478747619 1106 96-97 0.26382072631476916 1106 98-99 0.18718286462060263 1106 100 0.25385952575978266 1107 1 0.11674494256526557 1107 2 0.0946713407423374 1107 3 0.21440818382262705 1107 4 0.12902784948627044 1107 5 0.14332352190660202 1107 6 0.13097420406396765 1107 7 0.1249247454333684 1107 8 0.09563044767436679 1107 9 0.13521155165121712 1107 10-11 0.1498725965564347 1107 12-13 0.08088766715778917 1107 14-15 0.1992521476581075 1107 16-17 0.1333790412209268 1107 18-19 0.1585371111488243 1107 20-21 0.12775823002063902 1107 22-23 0.1221524066886559 1107 24-25 0.13214714113408377 1107 26-27 0.2066522562876827 1107 28-29 0.20571693604149743 1107 30-31 0.18369197817890637 1107 32-33 0.1670176558446954 1107 34-35 0.09177287062762929 1107 36-37 0.15991086266951982 1107 38-39 0.11176999555367928 1107 40-41 0.18361617936266583 1107 42-43 0.22901167725171945 1107 44-45 0.18057832887088665 1107 46-47 0.23897803236835813 1107 48-49 0.17263442532583184 1107 50-51 0.20021734856237572 1107 52-53 0.1327617667728873 1107 54-55 0.14146398780072644 1107 56-57 0.12178006316101886 1107 58-59 0.23301501540250769 1107 60-61 0.2027394452092679 1107 62-63 0.1946752538151415 1107 64-65 0.19682283187011507 1107 66-67 0.17030905431939658 1107 68-69 0.1348871464790733 1107 70-71 0.0694048974851853 1107 72-73 0.17885356069513847 1107 74-75 0.1362812504438793 1107 76-77 0.12049524696087133 1107 78-79 0.1478411875604877 1107 80-81 0.14121677495851515 1107 82-83 0.16543454985450978 1107 84-85 0.13779057398041772 1107 86-87 0.12022351700891676 1107 88-89 0.17154909942529173 1107 90-91 0.20343372172114016 1107 92-93 0.22494796774458337 1107 94-95 0.2413541608575258 1107 96-97 0.18008410791718887 1107 98-99 0.24220560848209516 1107 100 0.24850603169935326 1108 1 0.10042284521892597 1108 2 0.09959254955603569 1108 3 0.21215577062936575 1108 4 0.09710275227637055 1108 5 0.08143944045493612 1108 6 0.06761862899063686 1108 7 0.06625545523622378 1108 8 0.04295883808298839 1108 9 0.1658168538546434 1108 10-11 0.14216587628220623 1108 12-13 0.10708492652063484 1108 14-15 0.17766449246335014 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-0.49259444377437944 2215 10-11 -0.3835249047567828 2215 12-13 -0.39856341789550953 2215 14-15 -0.4043041309319264 2215 16-17 -0.4736300993818432 2215 18-19 -0.4718086435733184 2215 20-21 -0.49154445361218535 2215 22-23 -0.44732034659988074 2215 24-25 -0.45591665704490936 2215 26-27 -0.503441222175411 2215 28-29 -0.46312755470025024 2215 30-31 -0.4815664469890706 2215 32-33 -0.42037944055706333 2215 34-35 -0.5198436768455039 2215 36-37 -0.5061045485892066 2215 38-39 -0.5362256584877727 2215 40-41 -0.5359672406071283 2215 42-43 -0.5164004090819674 2215 44-45 -0.538238358406133 2215 46-47 -0.5569920725803215 2215 48-49 -0.5841502356428876 2215 50-51 -0.6137080492357825 2215 52-53 -0.5496219511351441 2215 54-55 -0.4691127511298703 2215 56-57 -0.4981833374934084 2215 58-59 -0.45263159127524233 2215 60-61 -0.42365448987357013 2215 62-63 -0.4083541952863925 2215 64-65 -0.4023320864362674 2215 66-67 -0.3363673680689274 2215 68-69 -0.41820293674665265 2215 70-71 -0.5048112021230367 2215 72-73 -0.43090206976295775 2215 74-75 -0.37299990858783616 2215 76-77 -0.29187493513985885 2215 78-79 -0.385498518112648 2215 80-81 -0.4332060552084869 2215 82-83 -0.4063219861826113 2215 84-85 -0.4535670627045576 2215 86-87 -0.3642988789726118 2215 88-89 -0.3849563983422257 2215 90-91 -0.4017481874911404 2215 92-93 -0.4104586673316959 2215 94-95 -0.5132248611718495 2215 96-97 -0.5485438167473902 2215 98-99 -0.39704375268799197 2215 100 -0.36324953309742725 2216 1 -0.4417950504907182 2216 2 -0.44808353988811334 2216 3 -0.5400064690059239 2216 4 -0.2554291519318923 2216 5 -0.4386218339705579 2216 6 -0.2764164478661115 2216 7 -0.5219182137305296 2216 8 -0.2963069236851652 2216 9 -0.6085464035048247 2216 10-11 -0.39173293836036294 2216 12-13 -0.35144749136544107 2216 14-15 -0.6314923420888263 2216 16-17 -0.39776952365792084 2216 18-19 -0.42333041357363754 2216 20-21 -0.46535950940845083 2216 22-23 -0.4645135130925482 2216 24-25 -0.3860177282361974 2216 26-27 -0.5272354559966246 2216 28-29 -0.5818553255864245 2216 30-31 -0.652458668620234 2216 32-33 -0.6500253851279325 2216 34-35 -0.6618692282783556 2216 36-37 -0.724923939844416 2216 38-39 -0.595868637950538 2216 40-41 -0.6122571316118197 2216 42-43 -0.6424233897734553 2216 44-45 -0.8184850822559824 2216 46-47 -0.6937471765393681 2216 48-49 -0.7163564186290543 2216 50-51 -0.6399675120141097 2216 52-53 -0.6003905294136302 2216 54-55 -0.5511103832739934 2216 56-57 -0.7059213729511029 2216 58-59 -0.6637385514802574 2216 60-61 -0.4767616316270775 2216 62-63 -0.38643384725039454 2216 64-65 -0.5258692599156873 2216 66-67 -0.39870621389139416 2216 68-69 -0.44804285493762563 2216 70-71 -0.5595162971058301 2216 72-73 -0.5744052088780336 2216 74-75 -0.49719227531063837 2216 76-77 -0.4500347811381715 2216 78-79 -0.6592634595352109 2216 80-81 -0.7736548662589939 2216 82-83 -0.6628916687983235 2216 84-85 -0.769165228200265 2216 86-87 -0.6768613724887373 2216 88-89 -1.0762905985834053 2216 90-91 -0.7852223358686814 2216 92-93 -0.8396854271012835 2216 94-95 -0.7642697536811625 2216 96-97 -0.8325439453212446 2216 98-99 -1.0191878127965452 2216 100 -0.9876092389704745 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 228.0 3 19.0 4 42.0 5 71.0 6 207.0 7 588.0 8 1452.0 9 2412.0 10 3493.0 11 4637.0 12 6814.0 13 9420.0 14 11594.0 15 14864.0 16 18186.0 17 24695.0 18 24411.0 19 13554.0 20 10420.0 21 10143.0 22 11128.0 23 11892.0 24 14171.0 25 17868.0 26 22650.0 27 29452.0 28 38988.0 29 51086.0 30 66594.0 31 86132.0 32 112267.0 33 146014.0 34 199690.0 35 289341.0 36 467204.0 37 859221.0 38 1233846.0 39 512070.0 40 1908.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.32677869211613 20.610074340576347 14.936035859483185 23.12711110782434 2 15.303947632261528 20.64770332094183 40.07813301324256 23.970216033554088 3 17.81660018129853 25.505062405689188 30.92708509480287 25.75125231820941 4 12.455434789890612 17.345601024217277 38.58163833644161 31.617325849450502 5 12.50580996180903 37.9572312886888 35.88738330408716 13.649575445415005 6 30.462265048840642 38.53023444062196 17.212086938281804 13.795413572255596 7 26.23178120723383 33.14889303479139 22.57887456304005 18.04045119493473 8 24.733681471188532 36.522132858368884 20.22851888942407 18.515666781018517 9 24.591801092781047 16.604847748968993 21.708281240037593 37.09506991821237 10-11 23.630477650474546 27.23474463427503 28.80220995700397 20.33256775824645 12-13 24.28600433408534 24.657439584715448 29.25415159175888 21.802404489440335 14-15 21.479772073717047 26.640004731138283 26.96902444298239 24.911198752162278 16-17 20.511292869120027 30.035126594231183 27.630887885858606 21.822692650790184 18-19 20.346352348045208 28.389351157638043 30.62662589450909 20.63767059980766 20-21 21.411884886837857 27.485624368974392 30.53801816291084 20.564472581276906 22-23 21.046858092780123 27.342789132807177 30.64519221617586 20.96516055823684 24-25 20.97619923266851 27.518695850643876 30.50766407439715 20.997440842290462 26-27 20.768352388404185 27.546099133494995 30.573726618352698 21.11182185974812 28-29 20.75517573879227 27.499645394494582 30.72833429665904 21.016844570054108 30-31 21.084293651871707 27.776168391405232 30.3255288104802 20.81400914624286 32-33 20.79976907947353 27.611451033805974 30.67355054401235 20.915229342708148 34-35 20.9546269442159 27.409244128152356 30.625088723250336 21.011040204381406 36-37 21.21166303726729 27.686181950783013 30.1203609940423 20.981794017907397 38-39 21.101669601689355 27.880832765618397 30.10490078329645 20.912596849395797 40-41 21.023079567285258 27.653921535107944 30.165954993157403 21.1570439044494 42-43 21.00448166329772 27.648709211666183 30.069673693329484 21.27713543170661 44-45 21.176456999233036 27.75614725695118 29.853942006486843 21.213453737328937 46-47 21.2550990282219 27.988299667849915 29.46701207688288 21.289589227045305 48-49 21.306389492350185 27.948888958706615 29.198619826776063 21.546101722167137 50-51 21.379155167488825 27.987007339183133 29.020815817216583 21.61302167611146 52-53 21.325060727762896 28.090058434950542 29.03381927094343 21.551061566343133 54-55 21.449203413317537 28.3511489054405 28.8247382241134 21.374909457128563 56-57 21.358884518367777 28.117488447586126 28.895761800926778 21.627865233119316 58-59 21.245063260493517 27.774773330425656 29.217747405266348 21.76241600381448 60-61 21.42290253951214 28.09335088568574 28.926694903360996 21.557051671441123 62-63 21.22902588727546 28.13264586440962 28.971772083676296 21.666556164638628 64-65 21.25349956442421 27.98688213441608 29.033823366683198 21.72579493447651 66-67 21.212802708613584 27.99731975515102 28.935509409387418 21.85436812684798 68-69 21.533245554994316 28.02859237511252 28.857211732881403 21.58095033701176 70-71 21.541174882030017 27.95795875922134 28.828649012838465 21.672217345910173 72-73 21.388731285495417 28.091751202662767 28.824402919287145 21.69511459255467 74-75 21.496021118439153 28.135510446599916 28.66507697756085 21.70339145740008 76-77 21.545163436145785 28.125000360959586 28.542708564467784 21.787127638426846 78-79 21.551292568616994 28.185240638548603 28.632033375925726 21.63143341690868 80-81 21.51742123935552 28.074888423719802 28.64540077479068 21.762289562133994 82-83 21.42842704383809 28.124771511159498 28.739827373612826 21.70697407138959 84-85 21.442902854909235 28.081302041352856 28.593748303454497 21.882046800283415 86-87 21.526696977460496 28.180675494390822 28.530664209276157 21.761963318872525 88-89 21.367880727653443 28.107623533863602 28.587099790086196 21.937395948396755 90-91 21.263139826559602 28.010383876299084 28.646785567918194 22.079690729223124 92-93 21.39063821136333 28.179725365355562 28.478154902440938 21.95148152084017 94-95 21.260165949557184 28.017759534279467 28.613910095043334 22.108164421120016 96-97 21.26874671085005 28.02452787558916 28.594789663047816 22.11193575051298 98-99 21.255533640781884 28.11788928964368 28.4929611222931 22.13361594728133 100 21.338194825620185 28.026373664960847 28.45948171188104 22.17594979753793 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 563.0 1 423.5 2 525.5 3 752.5 4 470.5 5 289.0 6 811.5 7 2322.0 8 4003.5 9 4520.5 10 3372.0 11 2104.0 12 1900.0 13 2113.5 14 2472.5 15 2913.0 16 3435.0 17 3943.5 18 4514.0 19 5306.0 20 6459.5 21 8053.0 22 10403.0 23 13841.5 24 18566.0 25 24785.5 26 33102.0 27 43124.0 28 55005.0 29 69041.0 30 83835.5 31 96390.0 32 105447.0 33 114613.5 34 124037.5 35 130952.0 36 136672.5 37 143837.0 38 150760.5 39 154440.0 40 156940.0 41 158758.5 42 158173.5 43 156281.5 44 153153.5 45 149682.0 46 146007.0 47 140248.0 48 133709.0 49 129538.5 50 126277.5 51 121495.5 52 114064.0 53 106656.0 54 100865.5 55 95364.0 56 88776.5 57 80522.0 58 73151.5 59 66677.5 60 58478.0 61 49107.5 62 41527.0 63 34677.0 64 27833.0 65 22175.0 66 17596.5 67 13537.0 68 10231.0 69 7794.5 70 5757.5 71 4046.5 72 2950.0 73 2165.0 74 1570.0 75 1078.5 76 742.0 77 524.5 78 352.0 79 219.5 80 119.0 81 52.5 82 26.5 83 13.0 84 6.5 85 3.5 86 1.5 87 2.0 88 1.5 89 0.0 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03834805806358015 2 0.0 3 0.0 4 6.930371939201233E-5 5 0.0 6 0.0 7 0.0 8 6.237334745281109E-4 9 0.0 10-11 0.0 12-13 0.044065614913421175 14-15 9.240495918934978E-5 16-17 1.1550619898668722E-5 18-19 2.3101239797337445E-5 20-21 1.2705681888535594E-4 22-23 0.0 24-25 9.240495918934978E-5 26-27 6.930371939201233E-5 28-29 4.158223163520739E-4 30-31 0.0 32-33 2.3101239797337445E-4 34-35 5.775309949334361E-5 36-37 5.775309949334361E-5 38-39 3.4651859696006166E-4 40-41 1.8480991837869956E-4 42-43 5.082272755414238E-4 44-45 2.7721487756804934E-4 46-47 1.3860743878402467E-4 48-49 9.240495918934978E-4 50-51 4.735754158454176E-4 52-53 6.237334745281109E-4 54-55 4.966766556427551E-4 56-57 5.313285153387612E-4 58-59 9.240495918934978E-5 60-61 1.7325929848003083E-4 62-63 9.933533112855102E-4 64-65 4.3892355614941143E-4 66-67 0.0014553781072322589 68-69 0.0015131312067256026 70-71 5.197778954400924E-4 72-73 0.0010164545510828475 74-75 9.933533112855102E-4 76-77 7.507902934134669E-4 78-79 0.0033034772910192542 80-81 0.0027490475358831557 82-83 0.0013745237679415778 84-85 0.0028414524950725055 86-87 0.001409175627637584 88-89 0.002090662201659039 90-91 0.0031879710920325673 92-93 0.0025873388573017936 94-95 0.003869457666054022 96-97 0.003372781010411267 98-99 0.003107116752741886 100 0.0013860743878402466 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4328772.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.64689160209188 #Duplication Level Percentage of deduplicated Percentage of total 1 76.66541296691668 47.26184402801122 2 14.06191758394285 17.337470180297494 3 4.239833956714563 7.841177530213529 4 1.7733737339919273 4.37291913397589 5 0.9357625728413634 2.884342694662307 6 0.5228427246915703 1.9338977264402164 7 0.3632321358528501 1.5674492473721873 8 0.25508284914451246 1.2580051800611614 9 0.18723944768275705 1.0388456941441029 >10 0.9336958939086 10.120881011716397 >50 0.047232008440752156 1.9717268700585489 >100 0.013587001156477445 1.3948905369080582 >500 5.6223198998766E-4 0.2574302712369438 >1k 1.1244636255417768E-4 0.1228113792166739 >5k 3.748212085136205E-5 0.1427985557024782 >10k+ 7.49642417027241E-5 0.4935099599827718 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11135 0.25723230514335244 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10223 0.23616397444818069 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6180 0.1427656619475454 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.3101239797337445E-5 0.0 3 0.0 0.0 0.0 6.930371939201233E-5 0.0 4 0.0 0.0 0.0 9.240495918934978E-5 0.0 5 0.0 0.0 0.0 9.240495918934978E-5 0.0 6 0.0 0.0 0.0 9.240495918934978E-5 0.0 7 0.0 0.0 0.0 9.240495918934978E-5 0.0 8 0.0 0.0 0.0 1.1550619898668722E-4 0.0 9 0.0 0.0 0.0 1.1550619898668722E-4 0.0 10-11 0.0 0.0 0.0 1.1550619898668722E-4 0.0 12-13 0.0 0.0 0.0 1.1550619898668722E-4 0.0 14-15 1.1550619898668722E-5 0.0 0.0 1.617086785813621E-4 0.0 16-17 2.3101239797337445E-5 0.0 0.0 2.07911158176037E-4 0.0 18-19 4.620247959467489E-5 0.0 0.0 2.07911158176037E-4 0.0 20-21 4.620247959467489E-5 0.0 0.0 2.541136377707119E-4 0.0 22-23 4.620247959467489E-5 0.0 0.0 3.9272107655473657E-4 0.0 24-25 4.620247959467489E-5 0.0 0.0 5.890816148321048E-4 0.0 26-27 5.775309949334361E-5 1.1550619898668722E-5 0.0 8.893977321974915E-4 0.0 28-29 6.930371939201233E-5 2.3101239797337445E-5 0.0 0.0021599659210510508 0.0 30-31 6.930371939201233E-5 2.3101239797337445E-5 0.0 0.006029423587105073 0.0 32-33 8.085433929068105E-5 2.3101239797337445E-5 0.0 0.015535583763709431 0.0 34-35 9.240495918934978E-5 2.3101239797337445E-5 0.0 0.030331927853904067 0.0 36-37 9.240495918934978E-5 2.3101239797337445E-5 0.0 0.05442652096252702 0.0 38-39 9.240495918934978E-5 2.3101239797337445E-5 0.0 0.08643328870173804 0.0 40-41 9.240495918934978E-5 2.3101239797337445E-5 0.0 0.11828989838226638 0.0 42-43 9.240495918934978E-5 2.3101239797337445E-5 0.0 0.15198305662668304 0.0 44-45 9.240495918934978E-5 2.3101239797337445E-5 0.0 0.19435073041499992 0.0 46-47 9.240495918934978E-5 2.3101239797337445E-5 0.0 0.2428864352292059 0.0 48-49 9.240495918934978E-5 2.3101239797337445E-5 0.0 0.29263495513277205 0.0 50-51 1.1550619898668722E-4 2.3101239797337445E-5 0.0 0.34238347503633826 0.0 52-53 1.1550619898668722E-4 2.3101239797337445E-5 0.0 0.39275572841443257 0.0 54-55 1.1550619898668722E-4 2.3101239797337445E-5 0.0 0.44651231342283676 0.0 56-57 1.1550619898668722E-4 2.3101239797337445E-5 0.0 0.5114036960135577 0.0 58-59 1.1550619898668722E-4 2.3101239797337445E-5 0.0 0.5817007687168555 0.0 60-61 1.1550619898668722E-4 2.3101239797337445E-5 0.0 0.651454962284916 0.0 62-63 1.1550619898668722E-4 2.3101239797337445E-5 0.0 0.7228493438785872 0.0 64-65 1.3860743878402467E-4 2.3101239797337445E-5 0.0 0.7988639734317262 0.0 66-67 1.3860743878402467E-4 2.3101239797337445E-5 0.0 0.8880463096693474 0.0 68-69 1.3860743878402467E-4 2.3101239797337445E-5 0.0 0.9851061686778606 0.0 70-71 1.3860743878402467E-4 2.3101239797337445E-5 0.0 1.0855850111763798 0.0 72-73 1.3860743878402467E-4 2.3101239797337445E-5 0.0 1.187322871243854 0.0 74-75 1.3860743878402467E-4 2.3101239797337445E-5 0.0 1.2921447468242726 0.0 76-77 1.3860743878402467E-4 2.3101239797337445E-5 0.0 1.4034696214076416 0.0 78-79 1.617086785813621E-4 2.3101239797337445E-5 0.0 1.5304917884333016 0.0 80-81 1.617086785813621E-4 2.3101239797337445E-5 0.0 1.6691223284571237 0.0 82-83 1.617086785813621E-4 2.3101239797337445E-5 0.0 1.8088732786111166 0.0 84-85 1.8480991837869956E-4 2.3101239797337445E-5 0.0 1.9502644167907204 0.0 86-87 2.07911158176037E-4 2.3101239797337445E-5 0.0 2.103598895945548 0.0 88 2.07911158176037E-4 2.3101239797337445E-5 0.0 2.2249728098407586 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7070 0.0 54.59768 1 GTATCAA 13275 0.0 43.173733 1 ATCAACG 16390 0.0 34.49383 3 TCAACGC 16470 0.0 34.29775 4 TATCAAC 17055 0.0 33.259083 2 CAACGCA 17110 0.0 33.09724 5 AACGCAG 17740 0.0 31.974943 6 GTGGTAT 3265 0.0 28.512312 1 ACGCAGA 20380 0.0 27.648634 7 CGCAGAG 20820 0.0 26.99655 8 TGGTATC 3170 0.0 26.833296 2 GTACATG 11570 0.0 24.300669 1 GCAGAGT 22925 0.0 24.16891 9 TACATGG 11860 0.0 22.66554 2 GAGTACT 16445 0.0 21.299297 12-13 AGAGTAC 21010 0.0 21.066126 10-11 ACATGGG 12280 0.0 20.818779 3 CAGAGTA 22255 0.0 20.669611 10-11 AGTACTT 17105 0.0 19.63912 12-13 GTACTTT 17935 0.0 19.521566 14-15 >>END_MODULE