FastQCFastQC Report
Wed 25 May 2016
SRR2049361_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049361_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5495905
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54550.044.5579341
GTATCAA90100.035.534611
TCAACGC94700.032.367644
ATCAACG95850.032.0283433
CAACGCA100400.030.4366635
AACGCAG106250.028.6660966
TATCAAC108950.028.6519552
ACGCAGA123500.024.0910267
CGCAGAG126100.023.2588398
GTACATG135750.023.1348081
TACATGG136750.021.9334432
ACATGGG135950.021.0596683
GCAGAGT143800.019.5134479
GTGGTAT34950.018.6980741
CATGGGG99000.018.2351554
CAGAGTA139250.017.83891110-11
GAGTACT66800.016.95739412-13
AGAGTAC126350.016.88881510-11
TGGTATC34450.016.5123772
GAGTACA104000.016.0481241
Produced by FastQC (version 0.11.5)