##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049361_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5495905
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.766479951891455	31.0	31.0	34.0	28.0	34.0
2	31.054011850641523	31.0	31.0	34.0	28.0	34.0
3	31.13023187263972	31.0	31.0	34.0	28.0	34.0
4	34.73622979290945	37.0	35.0	37.0	32.0	37.0
5	34.69436480434069	37.0	35.0	37.0	32.0	37.0
6	34.76858533762865	37.0	35.0	37.0	32.0	37.0
7	34.71106232731461	37.0	35.0	37.0	32.0	37.0
8	34.68973863267287	37.0	35.0	37.0	32.0	37.0
9	36.27687378147912	39.0	37.0	39.0	32.0	39.0
10-11	36.23925331678768	39.0	37.0	39.0	32.0	39.0
12-13	36.17354712281235	39.0	37.0	39.0	32.0	39.0
14-15	37.39541758818611	40.0	38.0	41.0	32.0	41.0
16-17	37.36698969505477	40.0	38.0	41.0	32.0	41.0
18-19	37.40493021622463	40.0	38.0	41.0	32.0	41.0
20-21	37.47847670219918	40.0	38.0	41.0	32.0	41.0
22-23	37.43221580431248	40.0	38.0	41.0	32.0	41.0
24-25	37.43569048227726	40.0	38.0	41.0	32.0	41.0
26-27	37.41915253265841	40.0	38.0	41.0	32.0	41.0
28-29	37.35461402626137	40.0	38.0	41.0	32.0	41.0
30-31	37.281916808969584	40.0	38.0	41.0	31.5	41.0
32-33	37.21634316823162	40.0	37.5	41.0	31.0	41.0
34-35	37.10309930757536	40.0	37.0	41.0	31.0	41.0
36-37	37.01866043899958	40.0	37.0	41.0	31.0	41.0
38-39	36.91142587071647	40.0	37.0	41.0	30.0	41.0
40-41	36.805649569998025	40.0	37.0	41.0	30.0	41.0
42-43	36.67435672559843	40.0	37.0	41.0	30.0	41.0
44-45	36.56671767434116	40.0	37.0	41.0	30.0	41.0
46-47	36.453557148458714	40.0	36.0	41.0	30.0	41.0
48-49	36.32797246313392	40.0	36.0	41.0	30.0	41.0
50-51	36.19545006691345	40.0	36.0	41.0	29.5	41.0
52-53	36.059559981477115	39.5	35.5	41.0	29.0	41.0
54-55	35.92562025726427	39.0	35.0	41.0	29.0	41.0
56-57	35.80216461165176	39.0	35.0	41.0	28.5	41.0
58-59	35.60266643983111	39.0	35.0	41.0	28.0	41.0
60-61	35.39090113457202	39.0	35.0	41.0	28.0	41.0
62-63	35.180702450278886	38.5	35.0	40.0	28.0	41.0
64-65	34.92300576520155	38.0	34.0	40.0	27.0	41.0
66-67	34.64746088223869	37.5	34.0	40.0	26.5	41.0
68-69	34.332482275439624	37.0	34.0	40.0	26.0	41.0
70-71	34.01318026785397	36.5	34.0	39.5	26.0	41.0
72-73	33.63712928080089	36.0	34.0	39.0	26.0	41.0
74-75	33.24423256952222	35.5	33.0	39.0	25.0	40.5
76-77	32.83582676556455	35.0	33.0	38.5	24.0	40.0
78-79	32.423656613424	35.0	33.0	37.0	23.0	39.0
80-81	31.96354149134674	35.0	33.0	37.0	21.0	39.0
82-83	31.551287822478734	35.0	32.0	36.0	19.0	39.0
84-85	31.164416142564328	35.0	32.0	36.0	15.5	37.0
86-87	30.737925328039694	35.0	32.0	35.5	4.5	37.0
88-89	30.360456194202776	35.0	31.5	35.0	2.0	37.0
90-91	30.02930072481238	35.0	31.0	35.0	2.0	36.0
92-93	29.68115433218005	35.0	31.0	35.0	2.0	36.0
94-95	29.381120397823473	35.0	31.0	35.0	2.0	36.0
96-97	29.03016200243636	34.0	30.5	35.0	2.0	35.5
98-99	28.728204817950818	34.0	30.0	35.0	2.0	35.0
100	28.56089306492743	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.36195092115635674
1101	2	0.4546525732290121
1101	3	0.4379564046275384
1101	4	0.512745840834576
1101	5	0.4083556945843583
1101	6	0.5463088560666947
1101	7	0.5633125125353544
1101	8	0.5749574883542721
1101	9	0.746347373794606
1101	10-11	0.7238408238251566
1101	12-13	0.7020076384600458
1101	14-15	0.7873057673560737
1101	16-17	0.7608862425807672
1101	18-19	0.6733340659391942
1101	20-21	0.7251247275226333
1101	22-23	0.7125619388559983
1101	24-25	0.737568703058102
1101	26-27	0.6810547173481751
1101	28-29	0.6797470460873285
1101	30-31	0.6465283531106678
1101	32-33	0.6550044061668459
1101	34-35	0.7002907188097964
1101	36-37	0.7424620926035601
1101	38-39	0.7815063623433502
1101	40-41	0.8174023077570993
1101	42-43	0.7816065129860803
1101	44-45	0.7053435519200093
1101	46-47	0.7177997538327858
1101	48-49	0.6853932665682976
1101	50-51	0.6950246397615558
1101	52-53	0.7299547293220598
1101	54-55	0.7064574246368878
1101	56-57	0.7059398381430526
1101	58-59	0.7650041854096443
1101	60-61	0.7973920511751373
1101	62-63	0.7466454055736662
1101	64-65	0.750139383456208
1101	66-67	0.7479284865832554
1101	68-69	0.7320652821546716
1101	70-71	0.8003851285508219
1101	72-73	0.8478939890970167
1101	74-75	0.8289160850560151
1101	76-77	0.8103456488493421
1101	78-79	0.7353597298483407
1101	80-81	0.8039695251862824
1101	82-83	0.800308365708915
1101	84-85	0.7678234484503683
1101	86-87	0.8188360189649337
1101	88-89	0.7952304605448504
1101	90-91	0.8474140015895237
1101	92-93	0.8192860765674759
1101	94-95	0.8255522021068096
1101	96-97	0.8127572691133551
1101	98-99	0.8871566431669891
1101	100	0.8629977268935356
1102	1	0.18951270996054603
1102	2	0.34900167087654665
1102	3	0.3511428801933292
1102	4	0.39764571192856835
1102	5	0.30722920399355047
1102	6	0.4403446545023826
1102	7	0.501880324899588
1102	8	0.5535987389850945
1102	9	0.5815734472114613
1102	10-11	0.5239860298693912
1102	12-13	0.5715384899618172
1102	14-15	0.650611310870346
1102	16-17	0.6076465782576577
1102	18-19	0.6368679820080203
1102	20-21	0.6000433055212611
1102	22-23	0.5805534262778451
1102	24-25	0.6335705057405079
1102	26-27	0.5805828780665152
1102	28-29	0.5152200960998954
1102	30-31	0.5480881369806099
1102	32-33	0.5898777777528821
1102	34-35	0.6143937305342178
1102	36-37	0.53662678661982
1102	38-39	0.5494029912222871
1102	40-41	0.5690466290711811
1102	42-43	0.4939521821522561
1102	44-45	0.45264636559053884
1102	46-47	0.39112885482060733
1102	48-49	0.4217750545759884
1102	50-51	0.45025068642001287
1102	52-53	0.42923712799823477
1102	54-55	0.3861016567099611
1102	56-57	0.3648068817487484
1102	58-59	0.4815871086964947
1102	60-61	0.439396408902887
1102	62-63	0.34671100362781715
1102	64-65	0.35955316033685136
1102	66-67	0.41153297533683997
1102	68-69	0.4129979661414609
1102	70-71	0.4348382732992633
1102	72-73	0.455654583114125
1102	74-75	0.41906860656995093
1102	76-77	0.4259202963525439
1102	78-79	0.47771141340025736
1102	80-81	0.40204368113412414
1102	82-83	0.3945463134630245
1102	84-85	0.40286980443180553
1102	86-87	0.3611577018280805
1102	88-89	0.40247175907668975
1102	90-91	0.50351251470493
1102	92-93	0.5114833797651492
1102	94-95	0.46868129027839345
1102	96-97	0.47570993877848977
1102	98-99	0.5778059379301297
1102	100	0.6461029291929776
1103	1	0.23465704032165036
1103	2	0.3194361237360006
1103	3	0.32992689955052157
1103	4	0.33908887086184336
1103	5	0.24130440135072462
1103	6	0.3790993253125663
1103	7	0.3972800961031595
1103	8	0.3958849518848311
1103	9	0.4902348863525603
1103	10-11	0.47673034821212923
1103	12-13	0.43261676683492567
1103	14-15	0.4855949157927526
1103	16-17	0.5074662812100925
1103	18-19	0.47953310822896356
1103	20-21	0.4391687227315231
1103	22-23	0.4613027552123157
1103	24-25	0.44982346432287557
1103	26-27	0.4976028162753394
1103	28-29	0.49168886457873384
1103	30-31	0.476882793387972
1103	32-33	0.5121103014324433
1103	34-35	0.4601981579302219
1103	36-37	0.4853682198253111
1103	38-39	0.469527270946827
1103	40-41	0.48045482519634675
1103	42-43	0.4976136823629602
1103	44-45	0.41153774217530525
1103	46-47	0.4209616747300373
1103	48-49	0.45282734429943616
1103	50-51	0.40583226409317064
1103	52-53	0.4706408742451629
1103	54-55	0.391121064996959
1103	56-57	0.33404286213402656
1103	58-59	0.4210612325576335
1103	60-61	0.3871941053173842
1103	62-63	0.35954677524616585
1103	64-65	0.39352061664767746
1103	66-67	0.46638526087408394
1103	68-69	0.4874420458254818
1103	70-71	0.40867223193735214
1103	72-73	0.3931847046433177
1103	74-75	0.39964289359217986
1103	76-77	0.47016321605203615
1103	78-79	0.3919490549286593
1103	80-81	0.3736358464383045
1103	82-83	0.44263722355882607
1103	84-85	0.4647203773849107
1103	86-87	0.4820192185383583
1103	88-89	0.46356546659156805
1103	90-91	0.45560255958438134
1103	92-93	0.448736061305258
1103	94-95	0.5515217868782436
1103	96-97	0.4245065832212873
1103	98-99	0.4559973367866661
1103	100	0.43329442594318834
1104	1	0.14254341485506927
1104	2	0.20321706339604617
1104	3	0.22934359225547496
1104	4	0.2237207195358124
1104	5	0.3083776442759927
1104	6	0.40071036936229376
1104	7	0.3444344295079702
1104	8	0.4197306337697455
1104	9	0.4785933451629987
1104	10-11	0.5115192616647803
1104	12-13	0.4275460635737858
1104	14-15	0.5675524095465008
1104	16-17	0.5915698891562045
1104	18-19	0.5088138516707446
1104	20-21	0.5084323651932365
1104	22-23	0.5051240597716742
1104	24-25	0.5827869010788476
1104	26-27	0.5060349872882739
1104	28-29	0.5303180340207518
1104	30-31	0.49575361120451333
1104	32-33	0.5229460265637869
1104	34-35	0.5730415741901069
1104	36-37	0.5021693642815492
1104	38-39	0.5811870970907833
1104	40-41	0.5935481227647728
1104	42-43	0.6323964799217876
1104	44-45	0.5676703096628231
1104	46-47	0.4824963877201469
1104	48-49	0.4853965960159101
1104	50-51	0.5624231080512416
1104	52-53	0.5272566600728368
1104	54-55	0.5505574461755387
1104	56-57	0.4938744790312981
1104	58-59	0.5569957705390962
1104	60-61	0.5418747125629153
1104	62-63	0.5974704920159866
1104	64-65	0.5579459598140133
1104	66-67	0.637443447723264
1104	68-69	0.6316575275834637
1104	70-71	0.5590769433710605
1104	72-73	0.5716085839155696
1104	74-75	0.7212954316293221
1104	76-77	0.7349719943983288
1104	78-79	0.7459330853889554
1104	80-81	0.7496631107793945
1104	82-83	0.689482884665388
1104	84-85	0.6283595223986325
1104	86-87	0.6970441349250756
1104	88-89	0.7406752911869461
1104	90-91	0.7144109796020182
1104	92-93	0.7017500404351615
1104	94-95	0.7887925849357416
1104	96-97	0.7044366424866517
1104	98-99	0.7254847425538742
1104	100	0.7195181006466385
1105	1	0.12814018233476077
1105	2	0.16512048974412608
1105	3	0.17096179445209003
1105	4	0.2001599039085491
1105	5	0.16783278876612684
1105	6	0.22985789600702589
1105	7	0.24822082302426196
1105	8	0.28364985388206776
1105	9	0.3040051520217162
1105	10-11	0.2579815171877442
1105	12-13	0.22602652912174648
1105	14-15	0.3360583850873198
1105	16-17	0.31027086672563087
1105	18-19	0.3087529558778286
1105	20-21	0.25881292249889043
1105	22-23	0.26245869833745417
1105	24-25	0.24976625898577254
1105	26-27	0.21411113186948683
1105	28-29	0.25607094764733773
1105	30-31	0.25142277617942455
1105	32-33	0.2693827344574302
1105	34-35	0.33763752044222883
1105	36-37	0.3259525963351706
1105	38-39	0.32732016154924537
1105	40-41	0.3324475724444511
1105	42-43	0.30196217775068845
1105	44-45	0.2663882085848428
1105	46-47	0.23879484936746564
1105	48-49	0.2963762429708581
1105	50-51	0.3493478780360064
1105	52-53	0.3217636111507147
1105	54-55	0.28352692526434
1105	56-57	0.311553988010985
1105	58-59	0.35238947426037015
1105	60-61	0.30011654789738174
1105	62-63	0.2622033220745763
1105	64-65	0.3003446895082007
1105	66-67	0.337842785622648
1105	68-69	0.31832300493797305
1105	70-71	0.3409344457101682
1105	72-73	0.2955423015924339
1105	74-75	0.2920706824951438
1105	76-77	0.37320349476076586
1105	78-79	0.2851627648994395
1105	80-81	0.34788591711948413
1105	82-83	0.3409069579992803
1105	84-85	0.35816868296254967
1105	86-87	0.30973889043486125
1105	88-89	0.340187989352323
1105	90-91	0.4197887104094953
1105	92-93	0.3945968924087033
1105	94-95	0.4169709501433907
1105	96-97	0.41835466575953006
1105	98-99	0.38747826306085287
1105	100	0.38559507925181435
1106	1	0.08781604069990578
1106	2	0.10731400394428192
1106	3	0.1407554503073456
1106	4	0.17189616850407674
1106	5	0.1741227045323086
1106	6	0.2568466307962467
1106	7	0.24709164896809455
1106	8	0.2272819981398797
1106	9	0.3635289179110046
1106	10-11	0.3293994854284463
1106	12-13	0.37429810477640757
1106	14-15	0.43846851150698996
1106	16-17	0.4693664645438389
1106	18-19	0.43167091303535443
1106	20-21	0.31237712955758923
1106	22-23	0.336759555546287
1106	24-25	0.4283109832322012
1106	26-27	0.3766673396738227
1106	28-29	0.408676478165404
1106	30-31	0.38244628139609915
1106	32-33	0.4424705695203457
1106	34-35	0.4401104805677818
1106	36-37	0.3844701502399417
1106	38-39	0.45193030894839126
1106	40-41	0.4214410445460146
1106	42-43	0.4568168494978053
1106	44-45	0.43429444463816225
1106	46-47	0.3791616393801931
1106	48-49	0.37388829526106804
1106	50-51	0.4081237919217173
1106	52-53	0.451263966248419
1106	54-55	0.43326859146412033
1106	56-57	0.3476641260723383
1106	58-59	0.3935342939093047
1106	60-61	0.42560758894541806
1106	62-63	0.37384773426438045
1106	64-65	0.35496048845011074
1106	66-67	0.40912291242106846
1106	68-69	0.41387426511158054
1106	70-71	0.3482307916334477
1106	72-73	0.31908713841592373
1106	74-75	0.40233371311488497
1106	76-77	0.4868709777419937
1106	78-79	0.315839576416991
1106	80-81	0.34743521618331386
1106	82-83	0.40107607840942805
1106	84-85	0.4730061819087723
1106	86-87	0.3882957712579973
1106	88-89	0.4098092626808594
1106	90-91	0.46136455182699265
1106	92-93	0.44151665324267597
1106	94-95	0.5029078242607774
1106	96-97	0.47310272092543215
1106	98-99	0.510762924707457
1106	100	0.4975530490877951
1107	1	0.13873345784657332
1107	2	0.17762105148167606
1107	3	0.13989272435530964
1107	4	0.178356392213459
1107	5	0.12760607149122905
1107	6	0.2592044195141554
1107	7	0.21928425269990726
1107	8	0.26564556047876664
1107	9	0.2993528128313301
1107	10-11	0.2672142787586722
1107	12-13	0.265022220162642
1107	14-15	0.2856329049025419
1107	16-17	0.3445963149921951
1107	18-19	0.295674481430261
1107	20-21	0.31766200292011604
1107	22-23	0.3294156536853734
1107	24-25	0.355556001485283
1107	26-27	0.3296916861863366
1107	28-29	0.39097515843200625
1107	30-31	0.3969789424445196
1107	32-33	0.3950155246049576
1107	34-35	0.37629216477306215
1107	36-37	0.3151950197919433
1107	38-39	0.3496607519555752
1107	40-41	0.3996894151510162
1107	42-43	0.3800588352106118
1107	44-45	0.3323701027753785
1107	46-47	0.3559271244448752
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2209	4	0.058933235312835563
2209	5	0.11676083205509258
2209	6	0.051876751146409106
2209	7	0.11330257322461534
2209	8	0.06729718025748355
2209	9	0.1306948161757262
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2210	34-35	-0.7148185803886946
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2212	78-79	-0.2548897255145661
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2213	100	-0.46403062545896745
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2214	3	-0.18698718607993214
2214	4	-0.18468291500472844
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2214	82-83	-0.056197919771314275
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2214	86-87	-0.07234086949405594
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2214	90-91	-0.15045571643937095
2214	92-93	-0.13923871404211852
2214	94-95	-0.11337506935908337
2214	96-97	-0.16861841056875448
2214	98-99	-0.1554056087540303
2214	100	-0.18440691369615436
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2215	2	-0.3184843228562606
2215	3	-0.3189894607323822
2215	4	-0.247349235791539
2215	5	-0.22363098459754127
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2215	10-11	-0.406697600138358
2215	12-13	-0.48331560948315655
2215	14-15	-0.5959877098133859
2215	16-17	-0.46864246212852834
2215	18-19	-0.5347989973153133
2215	20-21	-0.47466237972281533
2215	22-23	-0.5044611731024986
2215	24-25	-0.5816899758661549
2215	26-27	-0.5252135368930411
2215	28-29	-0.5238898810771815
2215	30-31	-0.5462714635355894
2215	32-33	-0.5378199221301116
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2215	80-81	-0.1101637029997633
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2215	86-87	0.051972758967050225
2215	88-89	0.07529757932506698
2215	90-91	0.03364176928876361
2215	92-93	0.038944163496893225
2215	94-95	0.049526077633462506
2215	96-97	0.05115939747207321
2215	98-99	-0.1060668815435335
2215	100	-0.0774558342728433
2216	1	-1.1455476920538388
2216	2	-1.197471076917715
2216	3	-1.2920616000482639
2216	4	-1.4740799118549006
2216	5	-1.2920734627124588
2216	6	-2.796696845830855
2216	7	-2.759473657608833
2216	8	-3.5203671568930126
2216	9	-3.017519671520539
2216	10-11	-2.199461329334426
2216	12-13	-1.9211377326976518
2216	14-15	-2.191556133847193
2216	16-17	-2.5653392458597324
2216	18-19	-2.210613243779541
2216	20-21	-2.011455885411742
2216	22-23	-2.214063649385608
2216	24-25	-2.393742597513679
2216	26-27	-2.372764707555014
2216	28-29	-2.842103832538804
2216	30-31	-2.416272632007505
2216	32-33	-2.599891553047719
2216	34-35	-3.5984807229956814
2216	36-37	-2.605059416423849
2216	38-39	-2.436515498398279
2216	40-41	-2.99709942057126
2216	42-43	-2.8535076727679254
2216	44-45	-2.6232839479825003
2216	46-47	-2.4483048209761478
2216	48-49	-2.6577784720710156
2216	50-51	-2.9525373985460206
2216	52-53	-3.3663618728286124
2216	54-55	-2.7684203053550505
2216	56-57	-2.773446365677472
2216	58-59	-3.78329666026211
2216	60-61	-4.462189233807976
2216	62-63	-3.588699426717149
2216	64-65	-3.5424859986571064
2216	66-67	-3.205406519766015
2216	68-69	-3.523355619209248
2216	70-71	-3.3502120295618596
2216	72-73	-3.79358344144611
2216	74-75	-4.205440147164275
2216	76-77	-4.849859967254268
2216	78-79	-3.7756194159482632
2216	80-81	-3.588460467083621
2216	82-83	-3.8304686839686894
2216	84-85	-3.8351420845788766
2216	86-87	-3.502800840958667
2216	88-89	-3.4597409918797055
2216	90-91	-3.5828469712746447
2216	92-93	-3.6322958444231936
2216	94-95	-3.456233963830673
2216	96-97	-3.7243650672923465
2216	98-99	-3.5063888493938045
2216	100	-3.190023746718005
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	97560.0
3	13289.0
4	8385.0
5	6904.0
6	6631.0
7	7345.0
8	8821.0
9	10206.0
10	11347.0
11	12586.0
12	14367.0
13	15989.0
14	18262.0
15	18418.0
16	18338.0
17	18862.0
18	18052.0
19	16581.0
20	16632.0
21	17582.0
22	19600.0
23	23078.0
24	28472.0
25	35439.0
26	44462.0
27	53550.0
28	68045.0
29	87424.0
30	106796.0
31	125580.0
32	154899.0
33	199660.0
34	265759.0
35	372519.0
36	595994.0
37	1036544.0
38	1395520.0
39	524993.0
40	1414.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.29711851534922	20.933544755890114	14.836443857065198	22.93289287169547
2	15.281085160519456	20.45670581887803	40.7547543487861	23.507454671816415
3	18.243600175479795	25.483197445713213	30.631126547507876	25.642075831299117
4	12.65254572207539	17.239471048010206	39.2874442985914	30.820538931323
5	13.026010770434498	37.74623688329505	35.856058760799286	13.371693585471164
6	30.370655163142263	38.30685276337076	17.916586341148527	13.405905732338445
7	26.275136008318324	32.772552733145545	23.15590996782693	17.796401290709202
8	24.157809285655386	36.86618278051953	20.90780623656466	18.068201697260424
9	24.347822129667385	17.406706908603716	22.170588920603485	36.07488204112542
10-11	23.39183149500345	27.395086678685953	29.310551659157035	19.902530167153554
12-13	23.842491501965384	25.005420210586333	29.233093448435366	21.91899483901292
14-15	21.351279742463248	27.271433992190126	26.464105892321	24.913180373025632
16-17	20.858945902994563	30.53074732502063	27.16460969734004	21.445697074644766
18-19	20.684235560873397	29.530492536669517	29.19372839589774	20.591543506559347
20-21	21.834070356277614	28.70159936375682	28.729441374046438	20.734888905919128
22-23	21.52021861763158	28.618856680902464	28.69227169124119	21.168653010224762
24-25	21.40159153115316	28.72347580807299	28.618727417638635	21.25620524313522
26-27	21.132826081020813	28.676080565744087	28.753233278421455	21.437860074813646
28-29	21.128730205595676	28.70676922483225	28.88367514332137	21.2808254262507
30-31	21.485596912696444	29.033129753887092	28.396352007287028	21.08492132612943
32-33	21.135917291589344	28.76533166788414	28.783061347786965	21.31568969273955
34-35	21.31881163968385	28.415653066648222	28.837606168610137	21.427929125057794
36-37	21.516123800528344	28.686041450929988	28.38351712020136	21.414317628340303
38-39	21.311057348997924	28.721980199677855	28.635208852825272	21.33175359849895
40-41	21.225736053882372	28.395241396439776	28.911444543315802	21.467578006362046
42-43	21.027056264546825	28.387530292755415	28.91058573381074	21.67482770888702
44-45	21.060499838134493	28.48652702880915	28.855172647286913	21.59780048576944
46-47	21.060378559840228	28.592258466375224	28.710045340028373	21.63731763375618
48-49	20.887764674039026	28.51916325092816	28.736671702941287	21.856400372091535
50-51	20.937850968285666	28.39796458872205	28.695767720656097	21.96841672233619
52-53	20.84224845079688	28.3593159196656	28.793457756199974	22.00497787333754
54-55	20.930881286756534	28.58477750447736	28.640449849292406	21.843891359473698
56-57	20.92968416530833	28.3527049767422	28.584236387745943	22.133374470203528
58-59	20.842511098413976	27.993859626928742	28.75163161110801	22.411997663549275
60-61	21.020749381146274	28.253385868665493	28.40411869577607	22.321746054412166
62-63	20.936285878652416	28.22161691509914	28.414552113936885	22.42754509231156
64-65	21.075642852579428	28.00861086543857	28.396507528500692	22.519238753481304
66-67	20.992875786775976	28.203620716644156	28.156823700295234	22.646679796284634
68-69	21.35852587809622	28.385416192653857	28.055967428448486	22.20009050080144
70-71	21.326671522442155	28.32581410158339	28.037616575698994	22.309897800275454
72-73	21.295698421248872	28.41713802225321	28.093976067868287	22.19318748862963
74-75	21.540908421165387	28.413327689560198	27.948426276453937	22.09733761282048
76-77	21.671648099260608	28.46452561990782	27.71573170723938	22.148094573592193
78-79	21.853295747288392	28.504593199381933	27.798298580439372	21.843812472890303
80-81	21.85319364708043	28.37518305584747	27.809153579612673	21.962469717459427
82-83	21.78782887641744	28.45224337733126	27.877886505043435	21.88204124120786
84-85	21.96456269358404	28.205639728211263	27.73347965320443	22.096317925000267
86-87	22.261084714023188	28.271781723345573	27.599996869239035	21.8671366933922
88-89	22.282882174307677	28.047911553166493	27.671016120534457	21.998190151991373
90-91	22.43988105173507	27.903231942381773	27.649592259807708	22.007294746075452
92-93	22.687646241341557	28.04136030499198	27.363492154501845	21.907501299164622
94-95	22.71512767348178	27.758240670105806	27.43224703980965	22.094384616602767
96-97	22.823056439294348	27.67853520029913	27.388455586477566	22.109952773928953
98-99	22.94410110800678	27.717955459564898	27.248797058901125	22.0891463735272
100	23.030056014432564	27.64736653926878	27.23833472376251	22.084242722536143
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1488.0
1	1240.0
2	1254.0
3	1582.0
4	1547.5
5	1304.5
6	1056.5
7	946.5
8	939.0
9	934.5
10	850.5
11	770.5
12	830.0
13	924.5
14	1023.5
15	1201.5
16	1451.0
17	1839.5
18	2439.0
19	3253.5
20	4569.5
21	6696.0
22	9686.5
23	13936.5
24	19851.5
25	27986.5
26	39121.0
27	51907.5
28	66052.5
29	83486.5
30	101241.5
31	116200.5
32	128333.0
33	141109.0
34	154593.5
35	164056.5
36	171837.0
37	180753.5
38	189882.5
39	196002.5
40	199385.0
41	202736.5
42	203984.5
43	203991.0
44	201845.0
45	197599.0
46	194044.5
47	188212.5
48	181275.0
49	176066.5
50	170834.5
51	163687.5
52	153844.5
53	144035.0
54	135359.0
55	126787.5
56	115938.5
57	103667.0
58	92205.0
59	81394.0
60	70101.0
61	58473.0
62	49137.0
63	40775.5
64	32792.5
65	26206.0
66	20852.0
67	16023.5
68	12276.0
69	9576.0
70	7216.5
71	5365.0
72	4113.5
73	3126.0
74	2301.5
75	1739.5
76	1369.5
77	1007.5
78	707.0
79	504.0
80	357.0
81	244.5
82	206.5
83	170.0
84	124.5
85	86.5
86	58.5
87	47.0
88	43.0
89	39.0
90	27.0
91	27.5
92	30.5
93	24.5
94	27.5
95	31.5
96	29.5
97	34.0
98	44.0
99	60.5
100	119.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.019359868847805776
2	0.04190392665084276
3	0.043577900273021455
4	0.04643457265000032
5	0.04947319868156382
6	0.04789020188667744
7	0.04532465535703401
8	0.04483338048965548
9	0.04485157585511394
10-11	0.04572495339712022
12-13	0.04687126142100345
14-15	0.04504262719242782
16-17	0.041203405080691896
18-19	0.044505863911403126
20-21	0.043814440023981494
22-23	0.04508811560607398
24-25	0.041203405080691896
26-27	0.04103054910883649
28-29	0.04197670811267662
30-31	0.04200400116086432
32-33	0.04170377763079966
34-35	0.041576410072590414
36-37	0.03879251915744541
38-39	0.03922920792844855
40-41	0.04065754411693797
42-43	0.04045739509689487
44-45	0.039056351956593134
46-47	0.03791914161543913
48-49	0.03812838831821147
50-51	0.039574919872159366
52-53	0.03991153413314095
54-55	0.039192817197531614
56-57	0.03730049918985136
58-59	0.03824665819369149
60-61	0.03781906710541758
62-63	0.03805560685637761
64-65	0.03486232021841717
66-67	0.035753893125881904
68-69	0.036954787246140534
70-71	0.03827395124187918
72-73	0.03476224570839562
74-75	0.03538088813398339
76-77	0.03645441469603277
78-79	0.03679102895701436
80-81	0.036781931274285126
82-83	0.035189836796669524
84-85	0.036490805426949706
86-87	0.03699117797705746
88-89	0.03772809027812526
90-91	0.037991923077272984
92-93	0.03620877726234351
94-95	0.037955532346356055
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5495905.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.870551328704494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.24348020655809	49.335836673407385
2	13.621556714538958	17.400326746256397
3	4.1988300589541705	8.04544772402819
4	1.823419567273115	4.658512522611265
5	0.9320345991590558	2.97647818528585
6	0.5679873367845679	2.176659860889175
7	0.33338353634186974	1.490537318904789
8	0.24242941736137275	1.2387280436133987
9	0.17552178849401817	1.0089606061181884
>10	0.8145478778582803	8.88587171221007
>50	0.035047197718928745	1.5144800525009705
>100	0.011561943658655122	1.1419069687539831
>500	1.1414693076879473E-4	0.04294172160917727
>1k	8.560836818277016E-5	0.08331186381126941
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.8195365458464073E-5	0.0
2	0.0	0.0	0.0	1.8195365458464073E-5	0.0
3	0.0	0.0	0.0	1.8195365458464073E-5	0.0
4	0.0	0.0	0.0	3.639073091692815E-5	0.0
5	0.0	1.8195365458464073E-5	0.0	3.639073091692815E-5	0.0
6	0.0	1.8195365458464073E-5	0.0	3.639073091692815E-5	0.0
7	0.0	1.8195365458464073E-5	0.0	5.458609637539223E-5	0.0
8	0.0	1.8195365458464073E-5	0.0	7.27814618338563E-5	0.0
9	0.0	1.8195365458464073E-5	0.0	1.2736755820924852E-4	0.0
10-11	0.0	1.8195365458464073E-5	0.0	2.183443855015689E-4	0.0
12-13	0.0	1.8195365458464073E-5	0.0	3.9120035735697764E-4	0.0
14-15	0.0	3.639073091692815E-5	0.0	4.457864537323698E-4	0.0
16-17	0.0	5.458609637539223E-5	0.0	5.185679155662261E-4	0.0
18-19	0.0	6.368377910462426E-5	0.0	6.368377910462426E-4	0.0
20-21	0.0	7.27814618338563E-5	0.0	9.097682729232037E-4	0.0
22-23	0.0	7.27814618338563E-5	0.0	0.0021925415377449207	0.0
24-25	0.0	7.27814618338563E-5	0.0	0.0049673347701606925	0.0
26-27	0.0	7.27814618338563E-5	0.0	0.01045323745588761	0.0
28-29	0.0	7.27814618338563E-5	0.0	0.031514372974059776	0.0
30-31	0.0	7.27814618338563E-5	0.0	0.09389718344840386	0.0
32-33	0.0	7.27814618338563E-5	0.0	0.23178166289264462	0.0
34-35	0.0	7.27814618338563E-5	0.0	0.44308444196178787	0.0
36-37	0.0	7.27814618338563E-5	0.0	0.7645328658337435	0.0
38-39	0.0	7.27814618338563E-5	0.0	1.1696071893528	0.0
40-41	0.0	9.097682729232037E-5	0.0	1.563418581653067	0.0
42-43	0.0	9.097682729232037E-5	0.0	1.9471679368548038	0.0
44-45	0.0	9.097682729232037E-5	0.0	2.3981036790119186	0.0
46-47	0.0	9.097682729232037E-5	0.0	2.9256419097491677	0.0
48-49	0.0	9.097682729232037E-5	0.0	3.4472848420778743	0.0
50-51	0.0	9.097682729232037E-5	0.0	3.941443674881571	0.0
52-53	0.0	9.097682729232037E-5	0.0	4.401531685864294	0.0
54-55	0.0	9.097682729232037E-5	0.0	4.89439864772044	0.0
56-57	0.0	9.097682729232037E-5	0.0	5.4434074096986755	0.0
58-59	0.0	9.097682729232037E-5	0.0	6.017607655154156	0.0
60-61	0.0	9.097682729232037E-5	0.0	6.582719315563133	9.097682729232037E-6
62-63	0.0	9.097682729232037E-5	0.0	7.110539574464989	1.8195365458464073E-5
64-65	0.0	9.097682729232037E-5	0.0	7.648303600589895	1.8195365458464073E-5
66-67	0.0	9.097682729232037E-5	0.0	8.23568638832003	1.8195365458464073E-5
68-69	0.0	9.097682729232037E-5	0.0	8.854146860253223	1.8195365458464073E-5
70-71	0.0	9.097682729232037E-5	0.0	9.47639196820178	1.8195365458464073E-5
72-73	0.0	1.1826987548001649E-4	0.0	10.078485708905085	1.8195365458464073E-5
74-75	0.0	1.2736755820924852E-4	0.0	10.664976923727757	1.8195365458464073E-5
76-77	0.0	1.2736755820924852E-4	0.0	11.269072154631495	1.8195365458464073E-5
78-79	0.0	1.2736755820924852E-4	0.0	11.914070567085858	1.8195365458464073E-5
80-81	0.0	1.455629236677126E-4	0.0	12.581958749286969	1.8195365458464073E-5
82-83	0.0	1.455629236677126E-4	0.0	13.230596234833026	1.8195365458464073E-5
84-85	0.0	1.5466060639694463E-4	0.0	13.851986160605033	1.8195365458464073E-5
86-87	0.0	1.6375828912617668E-4	0.0	14.482701211174502	1.8195365458464073E-5
88	0.0	1.6375828912617668E-4	0.0	14.975495391568813	1.8195365458464073E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5455	0.0	44.557934	1
GTATCAA	9010	0.0	35.53461	1
TCAACGC	9470	0.0	32.36764	4
ATCAACG	9585	0.0	32.028343	3
CAACGCA	10040	0.0	30.436663	5
AACGCAG	10625	0.0	28.666096	6
TATCAAC	10895	0.0	28.651955	2
ACGCAGA	12350	0.0	24.091026	7
CGCAGAG	12610	0.0	23.258839	8
GTACATG	13575	0.0	23.134808	1
TACATGG	13675	0.0	21.933443	2
ACATGGG	13595	0.0	21.059668	3
GCAGAGT	14380	0.0	19.513447	9
GTGGTAT	3495	0.0	18.698074	1
CATGGGG	9900	0.0	18.235155	4
CAGAGTA	13925	0.0	17.838911	10-11
GAGTACT	6680	0.0	16.957394	12-13
AGAGTAC	12635	0.0	16.888815	10-11
TGGTATC	3445	0.0	16.512377	2
GAGTACA	10400	0.0	16.048124	1
>>END_MODULE