FastQCFastQC Report
Wed 25 May 2016
SRR2049361_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049361_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5495905
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT177720.3233680349278235No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135780.24705667219502522No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130800.2379953801967101No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT82150.14947492724128236RNA PCR Primer, Index 46 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72070.13113399885915059No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90750.057.7593271
GTATCAA161800.040.2697261
ATCAACG198200.032.3902663
TCAACGC198550.032.380514
CAACGCA207100.031.04375
TATCAAC209650.030.7557772
AACGCAG213900.030.0360876
GTGGTAT43150.027.2366141
TGGTATC41050.026.789842
ACGCAGA245800.026.042327
CGCAGAG248150.025.6819788
GCAGAGT270700.023.0906529
GTACATG124450.023.0802361
TACATGG125750.021.7137892
GAGTACT199800.021.68359612-13
CAGAGTA264250.021.0289810-11
TTTCGCG3001.9099389E-1020.3808179
AGAGTAC249800.020.15550810-11
ACATGGG128500.019.9690283
GTACTTT218850.019.6166814-15
Produced by FastQC (version 0.11.5)