##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049361_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5495905 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.819018887699116 31.0 31.0 34.0 30.0 34.0 2 31.993926932870927 33.0 31.0 34.0 30.0 34.0 3 31.91903153347811 33.0 31.0 34.0 30.0 34.0 4 35.66123923175528 37.0 35.0 37.0 33.0 37.0 5 35.75596466823935 37.0 35.0 37.0 35.0 37.0 6 35.79718936189763 37.0 35.0 37.0 35.0 37.0 7 35.761609780372844 37.0 35.0 37.0 33.0 37.0 8 35.76407925537286 37.0 35.0 37.0 33.0 37.0 9 37.50298595044856 39.0 37.0 39.0 35.0 39.0 10-11 37.46093518719847 39.0 37.0 39.0 34.5 39.0 12-13 37.40576902257226 39.0 37.0 39.0 34.0 39.0 14-15 38.68113522704632 40.0 38.0 41.0 34.0 41.0 16-17 38.708784995373826 40.0 38.0 41.0 34.0 41.0 18-19 38.78215271188276 40.0 38.0 41.0 35.0 41.0 20-21 38.81930528275143 40.0 38.0 41.0 35.0 41.0 22-23 38.7848146028725 40.0 38.0 41.0 34.5 41.0 24-25 38.76168056034447 40.0 38.0 41.0 34.0 41.0 26-27 38.64281369492377 40.0 38.0 41.0 34.0 41.0 28-29 38.55698042451607 40.0 38.0 41.0 34.0 41.0 30-31 38.446897735677744 40.0 38.0 41.0 34.0 41.0 32-33 38.29502702466655 40.0 38.0 41.0 34.0 41.0 34-35 38.18366101670243 40.0 38.0 41.0 34.0 41.0 36-37 38.05185360736767 40.0 38.0 41.0 33.0 41.0 38-39 37.93598570208182 40.0 37.5 41.0 33.0 41.0 40-41 37.83660862041829 40.0 37.0 41.0 33.0 41.0 42-43 37.703256606509754 40.0 37.0 41.0 33.0 41.0 44-45 37.52620305481991 40.0 37.0 41.0 33.0 41.0 46-47 37.29875516407216 40.0 37.0 41.0 32.0 41.0 48-49 37.09097591752405 40.0 37.0 41.0 31.0 41.0 50-51 36.932497286616126 40.0 36.5 41.0 31.0 41.0 52-53 36.80316517479832 40.0 36.0 41.0 31.0 41.0 54-55 36.666289082507795 39.5 36.0 41.0 31.0 41.0 56-57 36.521687510974076 39.0 35.5 41.0 31.0 41.0 58-59 36.3366089843256 39.0 35.0 41.0 30.0 41.0 60-61 36.15078508453112 39.0 35.0 41.0 30.0 41.0 62-63 35.944219923743226 39.0 35.0 41.0 30.0 41.0 64-65 35.69617351464409 38.0 35.0 40.0 29.0 41.0 66-67 35.38654652873366 38.0 35.0 40.0 29.0 41.0 68-69 35.04477797560183 37.0 34.5 40.0 29.0 41.0 70-71 34.67523346928304 37.0 34.0 39.5 28.0 41.0 72-73 34.366967587685735 36.0 34.0 39.0 28.0 41.0 74-75 34.048825716601726 36.0 34.0 39.0 28.0 41.0 76-77 33.7028237387655 35.0 34.0 38.5 27.5 40.0 78-79 33.349136402466925 35.0 34.0 37.0 27.0 39.0 80-81 33.01165604208952 35.0 33.5 37.0 26.0 39.0 82-83 32.69686630318392 35.0 33.0 36.0 26.0 39.0 84-85 32.41555621867554 35.0 33.0 36.0 26.0 37.0 86-87 32.16373363440598 35.0 33.0 36.0 26.0 37.0 88-89 31.918168436317586 35.0 33.0 35.0 25.5 37.0 90-91 31.706410318227846 35.0 33.0 35.0 25.0 36.0 92-93 31.509635173824876 35.0 33.0 35.0 25.0 36.0 94-95 31.340276533164236 35.0 33.0 35.0 24.5 36.0 96-97 31.125399547481262 35.0 33.0 35.0 23.5 35.5 98-99 30.957326045482958 35.0 33.0 35.0 22.5 35.0 100 30.82900686966023 35.0 33.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1346666387981621 1101 2 0.1747135122427892 1101 3 0.37935595882076356 1101 4 0.17676918915974937 1101 5 0.18638367607353246 1101 6 0.1763954798202647 1101 7 0.10969946302019906 1101 8 0.12932119622558247 1101 9 0.2755733513314027 1101 10-11 0.3024454354435804 1101 12-13 0.22015019649983714 1101 14-15 0.3377479682597908 1101 16-17 0.22801226703586508 1101 18-19 0.3080608151445432 1101 20-21 0.32647930010402604 1101 22-23 0.32453787063128203 1101 24-25 0.34621552467633165 1101 26-27 0.4172433516135001 1101 28-29 0.418840962477411 1101 30-31 0.3612578289346473 1101 32-33 0.446896828875218 1101 34-35 0.4438289182148196 1101 36-37 0.5312405497687607 1101 38-39 0.38223578230593347 1101 40-41 0.42454862008942484 1101 42-43 0.4760004768439927 1101 44-45 0.47438573761126435 1101 46-47 0.5068538631104502 1101 48-49 0.454532805847073 1101 50-51 0.3896137865763478 1101 52-53 0.41657607241271677 1101 54-55 0.4350541099487657 1101 56-57 0.41803693988034496 1101 58-59 0.3777543966807215 1101 60-61 0.43733689603614323 1101 62-63 0.41978559312956065 1101 64-65 0.302610844501487 1101 66-67 0.2704220491991123 1101 68-69 0.34888021304479366 1101 70-71 0.2931422895764939 1101 72-73 0.27591212645373275 1101 74-75 0.3446403057499623 1101 76-77 0.2560716814310169 1101 78-79 0.36598592408699915 1101 80-81 0.4234761671536802 1101 82-83 0.34511973967193654 1101 84-85 0.43827359703163893 1101 86-87 0.3255292951696802 1101 88-89 0.4136111595718788 1101 90-91 0.49442517180058587 1101 92-93 0.48820610817985965 1101 94-95 0.5122501515388223 1101 96-97 0.5179368707632683 1101 98-99 0.5397160775730363 1101 100 0.5436729390090989 1102 1 0.20496925480400918 1102 2 0.19102512268657534 1102 3 0.3292229765020913 1102 4 0.17880308851326276 1102 5 0.1741090747963412 1102 6 0.17751045387191056 1102 7 0.162380053926384 1102 8 0.2080326233777754 1102 9 0.1481422894946789 1102 10-11 0.22457704571639425 1102 12-13 0.20068150268597407 1102 14-15 0.25824816727975985 1102 16-17 0.22352943505425316 1102 18-19 0.25601167045086726 1102 20-21 0.18122989509625143 1102 22-23 0.24664738784503726 1102 24-25 0.24877090362642207 1102 26-27 0.3393054160987461 1102 28-29 0.2671486376287149 1102 30-31 0.24016833513901048 1102 32-33 0.2173417005506053 1102 34-35 0.29107202589279524 1102 36-37 0.33173729869214696 1102 38-39 0.31111133577919503 1102 40-41 0.2690299737560835 1102 42-43 0.3134328588778317 1102 44-45 0.4068794617229372 1102 46-47 0.39045960047373285 1102 48-49 0.3276822971281135 1102 50-51 0.38921981251337456 1102 52-53 0.41634969457557247 1102 54-55 0.36033393335442554 1102 56-57 0.40468628586672395 1102 58-59 0.4166276770796671 1102 60-61 0.40563006361472276 1102 62-63 0.34023614140925673 1102 64-65 0.35128459026037007 1102 66-67 0.340149793904196 1102 68-69 0.365810342264254 1102 70-71 0.3973670384870971 1102 72-73 0.440695844180226 1102 74-75 0.3559238643508351 1102 76-77 0.3916611374521253 1102 78-79 0.3984396113707618 1102 80-81 0.4199196711618356 1102 82-83 0.49920377065246413 1102 84-85 0.4138732511323582 1102 86-87 0.3939066092416894 1102 88-89 0.34497917151905 1102 90-91 0.3644823211492394 1102 92-93 0.4213407481990501 1102 94-95 0.4474691669921853 1102 96-97 0.46352757400401856 1102 98-99 0.48128075301826456 1102 100 0.47183760508541184 1103 1 0.12314389755134414 1103 2 0.10458063240263726 1103 3 0.26715200605692146 1103 4 0.16111636792867756 1103 5 0.16681239148277882 1103 6 0.10650669799967716 1103 7 0.1350212658842267 1103 8 0.1890830793556404 1103 9 0.1737651261948514 1103 10-11 0.1608178361165855 1103 12-13 0.13371218596443413 1103 14-15 0.21298492104495637 1103 16-17 0.18424816024161572 1103 18-19 0.22357252051797616 1103 20-21 0.201270323013091 1103 22-23 0.22256925599774746 1103 24-25 0.18862968317518636 1103 26-27 0.22887525186723678 1103 28-29 0.2644250075489438 1103 30-31 0.21421515907426425 1103 32-33 0.18451380981228027 1103 34-35 0.20091397293319346 1103 36-37 0.24746005869982923 1103 38-39 0.2413692681292119 1103 40-41 0.25922970154637426 1103 42-43 0.3035399133260839 1103 44-45 0.29681785300884655 1103 46-47 0.2603759593023227 1103 48-49 0.19426601187306858 1103 50-51 0.2435319742140365 1103 52-53 0.2753409734207892 1103 54-55 0.2356752292038422 1103 56-57 0.18379340904107977 1103 58-59 0.23194443106593354 1103 60-61 0.21071995302767732 1103 62-63 0.1985499867419236 1103 64-65 0.2721589987075532 1103 66-67 0.1851995102851589 1103 68-69 0.21520331778734203 1103 70-71 0.2794408570797202 1103 72-73 0.3097573113155718 1103 74-75 0.2762017627348783 1103 76-77 0.27635490036551147 1103 78-79 0.26350559298388987 1103 80-81 0.3504110932679225 1103 82-83 0.3184767913726958 1103 84-85 0.2898747616813182 1103 86-87 0.3088018138718738 1103 88-89 0.34755807407808703 1103 90-91 0.313681163256156 1103 92-93 0.37256104415150304 1103 94-95 0.3060211009457916 1103 96-97 0.3685657539459797 1103 98-99 0.3959004897852054 1103 100 0.4222806182968135 1104 1 0.1652344041551359 1104 2 0.1844398727537353 1104 3 0.32465558444287623 1104 4 0.2157043742688174 1104 5 0.1354800557719571 1104 6 0.14439172782054044 1104 7 0.1705579179263239 1104 8 0.1585445553011695 1104 9 0.1585540043635021 1104 10-11 0.16617156412632283 1104 12-13 0.18070481670440586 1104 14-15 0.24094087563209143 1104 16-17 0.23688487497162924 1104 18-19 0.2220146533810663 1104 20-21 0.21354728989042826 1104 22-23 0.2179056438802789 1104 24-25 0.24093717463547648 1104 26-27 0.22213964548615905 1104 28-29 0.28572367055966197 1104 30-31 0.25295216427003453 1104 32-33 0.27759278207552285 1104 34-35 0.26125560049015206 1104 36-37 0.3029035060880503 1104 38-39 0.3087807199836021 1104 40-41 0.3784144293753755 1104 42-43 0.3143896736496927 1104 44-45 0.25995418529947045 1104 46-47 0.29054502848288166 1104 48-49 0.3091841510261233 1104 50-51 0.2773087072368483 1104 52-53 0.2798989844380273 1104 54-55 0.2850308531406682 1104 56-57 0.24477059558093828 1104 58-59 0.36699652397577864 1104 60-61 0.3560818669260897 1104 62-63 0.36716402718758445 1104 64-65 0.30720758673976434 1104 66-67 0.35145116912335084 1104 68-69 0.3597339327365461 1104 70-71 0.4177084831053648 1104 72-73 0.40438035859104104 1104 74-75 0.36357653490803443 1104 76-77 0.33311680901051943 1104 78-79 0.31488403312516766 1104 80-81 0.3772863458915765 1104 82-83 0.34609608168594974 1104 84-85 0.37239116188187893 1104 86-87 0.3328179410134595 1104 88-89 0.31417986210670534 1104 90-91 0.3482285929929674 1104 92-93 0.4381051724817695 1104 94-95 0.5006446343564086 1104 96-97 0.5263737156636239 1104 98-99 0.5246715205210997 1104 100 0.5016070702041304 1105 1 0.17194216983655508 1105 2 0.14149158299213127 1105 3 0.28308826674072307 1105 4 0.18458784350740842 1105 5 0.15856583905068078 1105 6 0.13247069513629128 1105 7 0.14030892263163253 1105 8 0.15125568267122702 1105 9 0.20413470860189875 1105 10-11 0.2056230955162519 1105 12-13 0.12919932817938928 1105 14-15 0.23731028953454825 1105 16-17 0.20147713285063418 1105 18-19 0.15233003186460792 1105 20-21 0.15918596933241247 1105 22-23 0.1853474560306907 1105 24-25 0.1848457812659703 1105 26-27 0.21091335175165682 1105 28-29 0.21884715051711368 1105 30-31 0.21634094363681555 1105 32-33 0.21480900880960263 1105 34-35 0.16845290361194998 1105 36-37 0.1846045611885856 1105 38-39 0.1804404502506216 1105 40-41 0.238488114037537 1105 42-43 0.21009393838757262 1105 44-45 0.157616660017176 1105 46-47 0.23476649948135275 1105 48-49 0.2274778117665477 1105 50-51 0.28089418980025016 1105 52-53 0.2099745509232136 1105 54-55 0.20601786178726655 1105 56-57 0.18040088209225047 1105 58-59 0.19524216812003203 1105 60-61 0.2414172642429122 1105 62-63 0.18736675974516004 1105 64-65 0.14016637707381818 1105 66-67 0.22051991512917368 1105 68-69 0.19948983018170452 1105 70-71 0.21181214445550722 1105 72-73 0.17948537560648248 1105 74-75 0.15929775110767252 1105 76-77 0.14287564962688037 1105 78-79 0.19095426727664488 1105 80-81 0.23888905692109574 1105 82-83 0.16533191099406253 1105 84-85 0.16414895771011118 1105 86-87 0.17713587425992472 1105 88-89 0.2529559620510682 1105 90-91 0.2968267477525899 1105 92-93 0.24008676223080272 1105 94-95 0.22373710939778846 1105 96-97 0.3256024768444732 1105 98-99 0.30822307757910394 1105 100 0.339551426955623 1106 1 0.09024133472424367 1106 2 0.1299979891502545 1106 3 0.2619651456648846 1106 4 0.17358033819578367 1106 5 0.10675691774657992 1106 6 0.10562673967770309 1106 7 0.15970786936418335 1106 8 0.12944907050582088 1106 9 0.1882990438762704 1106 10-11 0.1311985344489628 1106 12-13 0.1469697474320526 1106 14-15 0.2405963178142514 1106 16-17 0.20174644509557282 1106 18-19 0.19197810027793594 1106 20-21 0.2290549994540214 1106 22-23 0.15990229231484676 1106 24-25 0.2014600603303549 1106 26-27 0.24045549126378063 1106 28-29 0.2252496433396729 1106 30-31 0.28769075476044037 1106 32-33 0.23803042658584417 1106 34-35 0.21968676560364742 1106 36-37 0.2483818635246493 1106 38-39 0.2452390621144218 1106 40-41 0.22082176311024426 1106 42-43 0.18855188230464393 1106 44-45 0.20236534177475818 1106 46-47 0.2834723956741385 1106 48-49 0.24574457366119162 1106 50-51 0.2220195310003632 1106 52-53 0.22947765325016434 1106 54-55 0.26787005282477594 1106 56-57 0.24352501932875015 1106 58-59 0.23390483821392394 1106 60-61 0.22448737671000174 1106 62-63 0.23755985121410106 1106 64-65 0.25549528796148735 1106 66-67 0.25039760408452594 1106 68-69 0.27140396652630727 1106 70-71 0.3836850636344309 1106 72-73 0.3638801796778921 1106 74-75 0.32916295884550095 1106 76-77 0.3404255627156516 1106 78-79 0.36244302815931206 1106 80-81 0.3868215051020485 1106 82-83 0.36183791187477965 1106 84-85 0.35678165333368383 1106 86-87 0.30438556092045843 1106 88-89 0.3218121041069111 1106 90-91 0.35679964373539974 1106 92-93 0.3992796650240962 1106 94-95 0.38610228528242274 1106 96-97 0.37858137047905416 1106 98-99 0.43913116949573805 1106 100 0.44543791542240996 1107 1 0.1384391053421723 1107 2 0.11365912942622458 1107 3 0.226301440091202 1107 4 0.17532265959741977 1107 5 0.13145462769102068 1107 6 0.14306111874977745 1107 7 0.1042964180657151 1107 8 0.13061482366792632 1107 9 0.15627112613363892 1107 10-11 0.15832876923834505 1107 12-13 0.1494603373711385 1107 14-15 0.18265661081616713 1107 16-17 0.17351477387556002 1107 18-19 0.15598417783340324 1107 20-21 0.15341042871042987 1107 22-23 0.12456140371477176 1107 24-25 0.12375366840684165 1107 26-27 0.17968348068050233 1107 28-29 0.22525744016628124 1107 30-31 0.19499429741163965 1107 32-33 0.21861852562312123 1107 34-35 0.24056128035751811 1107 36-37 0.23540688084778338 1107 38-39 0.2037911744023333 1107 40-41 0.22448261419482662 1107 42-43 0.3008312462877285 1107 44-45 0.19591555163697905 1107 46-47 0.21827212780353022 1107 48-49 0.29131986038594704 1107 50-51 0.2711929823237824 1107 52-53 0.2718484584156968 1107 54-55 0.2945999843601257 1107 56-57 0.2898441800159546 1107 58-59 0.2960208515915852 1107 60-61 0.21870794354780543 1107 62-63 0.17689261580437687 1107 64-65 0.16643080508671915 1107 66-67 0.18574287308355508 1107 68-69 0.2212732633602883 1107 70-71 0.2275820763441061 1107 72-73 0.2721391879895947 1107 74-75 0.18728851383779244 1107 76-77 0.12441480763127544 1107 78-79 0.12233249467491447 1107 80-81 0.1811802317073159 1107 82-83 0.19079327036435956 1107 84-85 0.1721709588093816 1107 86-87 0.14072584675495392 1107 88-89 0.20904611891123892 1107 90-91 0.16973645031399798 1107 92-93 0.21565704107156236 1107 94-95 0.26511681459792413 1107 96-97 0.26918613771400857 1107 98-99 0.2675956397821757 1107 100 0.3083273172952339 1108 1 0.12449142846585559 1108 2 0.09239370927150503 1108 3 0.21504946676371972 1108 4 0.11207302550412379 1108 5 0.08489260112907004 1108 6 0.10314382399677413 1108 7 0.07043967480015567 1108 8 0.029452843008357377 1108 9 0.14409411105007308 1108 10-11 0.13751352183180643 1108 12-13 0.106717589471792 1108 14-15 0.17777651681122109 1108 16-17 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-0.3209494224078 2215 12-13 -0.237403943883443 2215 14-15 -0.3360469516071731 2215 16-17 -0.33244516982917816 2215 18-19 -0.32966942193863247 2215 20-21 -0.3422042810855146 2215 22-23 -0.33478970004594544 2215 24-25 -0.328925193357847 2215 26-27 -0.40244448042387404 2215 28-29 -0.38267349343346524 2215 30-31 -0.36706957131516305 2215 32-33 -0.3190960270777765 2215 34-35 -0.40627133910805213 2215 36-37 -0.394422792656421 2215 38-39 -0.4124006206060358 2215 40-41 -0.3772965988389174 2215 42-43 -0.38910999265908686 2215 44-45 -0.422516693213538 2215 46-47 -0.4307402161497862 2215 48-49 -0.33792594849172275 2215 50-51 -0.37229707576165794 2215 52-53 -0.2996925506024368 2215 54-55 -0.2555385589572552 2215 56-57 -0.2858660705955245 2215 58-59 -0.3269657764462792 2215 60-61 -0.36851236423686373 2215 62-63 -0.3223689749215737 2215 64-65 -0.28056636445576544 2215 66-67 -0.2389793229806827 2215 68-69 -0.27928358292835753 2215 70-71 -0.343639206479466 2215 72-73 -0.2821377345855467 2215 74-75 -0.2848241534282181 2215 76-77 -0.15385127229543372 2215 78-79 -0.25200842218536224 2215 80-81 -0.31885253116940504 2215 82-83 -0.2524008244213931 2215 84-85 -0.347078193794502 2215 86-87 -0.2407612825353027 2215 88-89 -0.35863810973636134 2215 90-91 -0.33309735459876677 2215 92-93 -0.3132247896571698 2215 94-95 -0.337964839270132 2215 96-97 -0.3964404915401971 2215 98-99 -0.36147250528478025 2215 100 -0.4164422635508416 2216 1 -0.37598964277695757 2216 2 -0.40337813082940244 2216 3 -0.4762087036717162 2216 4 -0.26193001166958396 2216 5 -0.46149569805592705 2216 6 -0.3650054683699011 2216 7 -0.6242861134563 2216 8 -0.40254970060760087 2216 9 -0.580785467895673 2216 10-11 -0.4636151907202972 2216 12-13 -0.4761573186104542 2216 14-15 -0.7541193238863144 2216 16-17 -0.5597397100278414 2216 18-19 -0.5051386268878701 2216 20-21 -0.5426515007504662 2216 22-23 -0.5350586579544299 2216 24-25 -0.5241986181124787 2216 26-27 -0.6110205558218169 2216 28-29 -0.652261632340192 2216 30-31 -0.7761389605346878 2216 32-33 -0.7480043978098578 2216 34-35 -0.6920911348168843 2216 36-37 -0.7823509822806685 2216 38-39 -0.7422734691936697 2216 40-41 -0.6995624160969527 2216 42-43 -0.8150711123210499 2216 44-45 -0.9006355943368263 2216 46-47 -0.8615161645844154 2216 48-49 -1.0273377385328715 2216 50-51 -0.8742353234166913 2216 52-53 -0.8268469938099443 2216 54-55 -0.7997592058028289 2216 56-57 -0.8619979313883803 2216 58-59 -0.8651521265806892 2216 60-61 -0.7866816439138091 2216 62-63 -0.6877642338102135 2216 64-65 -0.7823393112203121 2216 66-67 -0.6406568977115441 2216 68-69 -0.6065384030577974 2216 70-71 -0.7032460595473111 2216 72-73 -0.6489679939670339 2216 74-75 -0.6340187785105798 2216 76-77 -0.6813651967519334 2216 78-79 -0.9275264463963921 2216 80-81 -1.0347529082375502 2216 82-83 -0.9387830785100633 2216 84-85 -1.057034568184335 2216 86-87 -0.8715356391097302 2216 88-89 -1.2554132203008592 2216 90-91 -1.107208087983718 2216 92-93 -1.195485840639833 2216 94-95 -1.1268401757458442 2216 96-97 -1.2047037049270344 2216 98-99 -1.3481326336930657 2216 100 -1.3058531762410652 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 312.0 3 34.0 4 49.0 5 94.0 6 258.0 7 692.0 8 1714.0 9 3149.0 10 4870.0 11 7035.0 12 9773.0 13 13087.0 14 16356.0 15 20850.0 16 25053.0 17 33185.0 18 33595.0 19 19907.0 20 16302.0 21 16592.0 22 18034.0 23 21157.0 24 25928.0 25 31907.0 26 37239.0 27 41258.0 28 52509.0 29 67679.0 30 88537.0 31 113728.0 32 145991.0 33 189385.0 34 258256.0 35 371701.0 36 591778.0 37 1074914.0 38 1519674.0 39 621013.0 40 2310.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.441739559075934 21.132812933743438 15.08477411620324 23.34067339097739 2 15.190837541769737 20.680688621801142 40.45710397104754 23.67136986538159 3 17.422699264270396 25.76394606529771 31.43889859813807 25.374456072293828 4 12.384666317679303 17.975214619040628 38.594854601638566 31.0452644616415 5 12.446885453806061 37.7811661591676 36.14722234099752 13.624726046028815 6 29.838088540467854 38.927801699629086 17.334015053025844 13.900094706877212 7 25.60044251128795 33.38412872857155 22.69451528001303 18.320913480127476 8 24.134382271403627 36.769984737558055 20.624894010819812 18.470738980218506 9 24.11224357044017 16.82112409148266 21.976307814636534 37.09032452344063 10-11 23.304169195064325 27.347861726139733 29.057252627183328 20.29071645161261 12-13 24.1125904998637 24.784081398793745 29.29470974283453 21.80861835850802 14-15 21.407679886548184 26.81694713778986 26.92433625311516 24.851036722546795 16-17 20.39104569675058 30.277424737145203 27.678289562865444 21.653240003238775 18-19 20.202077912625775 28.455639658654636 30.661229280008733 20.681053148710856 20-21 21.217969648467978 27.581230773737627 30.502862997610674 20.697936580183722 22-23 20.85327166317467 27.681400970358837 30.558270202996596 20.90705716346989 24-25 20.918919892618096 27.82802737605392 30.49346075927901 20.75959197204897 26-27 20.723104561864712 27.802528048411368 30.60435330012858 20.870014089595344 28-29 20.584120983674687 27.597635829881355 30.90674981740874 20.911493369035217 30-31 20.950382234625803 28.188108072110786 30.292556939444637 20.568952753818774 32-33 21.001906606052902 27.663209872320078 30.611312104687656 20.72357141693936 34-35 20.87361147590335 27.35640951156576 30.719408300571782 21.05057071195911 36-37 20.939265719833486 27.90926216900436 30.193728979214413 20.957743131947737 38-39 20.86924616465563 27.573958241692857 30.486781163773607 21.070014429877897 40-41 20.725383085851696 27.391393505262833 30.67703610111982 21.20618730776565 42-43 20.83462938077302 27.30014948480011 30.588332907407946 21.27688822701893 44-45 20.720021754440296 27.57679170951308 30.34280438134719 21.360382154699433 46-47 20.959140799192415 27.619153412235814 29.848416036833292 21.57328975173848 48-49 20.808598406562908 27.610469380062845 29.693748316344927 21.887183897029317 50-51 20.69918049475998 27.58665268527662 29.668786255587598 22.045380564375805 52-53 20.58862408891772 27.664167667797667 29.83000408943629 21.917204153848324 54-55 20.71424977826566 27.86296130818758 29.44540167691116 21.977387236635597 56-57 20.73228402475821 27.666141484710437 29.25131275777297 22.35026173275838 58-59 20.431354596229625 27.54637278434672 29.630846362449365 22.391426256974295 60-61 20.729899315762037 28.091211713469782 28.907320827635903 22.271568143132285 62-63 20.3417648080449 28.227880081636066 28.897157139593645 22.533197970725393 64-65 20.366751327108606 28.005159868494133 28.95655497353334 22.671533830863922 66-67 20.3991707150472 28.11403541492178 28.68761689568015 22.799176974350864 68-69 20.81283362910406 28.1878416124572 28.469046697566565 22.53027806087218 70-71 20.777688225822313 28.04965575567218 28.419551123628366 22.753104894877133 72-73 20.60866488586794 28.062727711653622 28.493207190845965 22.83540021163247 74-75 20.867634985538174 27.932531976866898 28.31363785898009 22.886195178614837 76-77 20.991815928303104 27.921797841047034 28.109375450623407 22.97701078002645 78-79 21.021863583148615 28.035902159391846 28.117411131656418 22.824823125803125 80-81 20.875823328716738 27.944970977142447 28.13387133650166 23.045334357639156 82-83 20.805245788289774 27.86442466685014 28.25906879524731 23.071260749612776 84-85 20.93373569335457 27.617319701081282 28.105552271699825 23.343392333864323 86-87 21.026373376233764 27.780926126397915 28.051240336644547 23.141460160723778 88-89 20.875131214003147 27.558134012689344 28.163124047794376 23.403610725513133 90-91 20.79238205175701 27.371374430970214 28.220028975074957 23.61621454219782 92-93 20.815570400532337 27.456989123071402 28.15648278737012 23.57095768902614 94-95 20.724093382098733 27.159797659988715 28.366632094182727 23.749476863729825 96-97 20.783185007801954 27.184590387624887 28.342169118854542 23.690055485718617 98-99 20.791984355190667 27.229128313183683 28.244230430682606 23.734656900943047 100 20.83342127883187 27.205454755862085 28.202738513650715 23.75838545165533 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 470.0 1 391.0 2 525.5 3 709.5 4 458.5 5 318.5 6 890.5 7 2487.0 8 4286.0 9 4972.5 10 3796.0 11 2377.5 12 2244.0 13 2548.5 14 2922.0 15 3406.0 16 3954.5 17 4535.0 18 5233.0 19 6175.5 20 7463.0 21 9393.5 22 12252.5 23 16165.0 24 21594.0 25 29301.5 26 39763.5 27 52082.0 28 66016.5 29 82834.5 30 99806.5 31 114516.0 32 126034.5 33 137757.5 34 150956.0 35 160246.5 36 168031.0 37 177170.0 38 186118.5 39 192553.5 40 197602.5 41 202779.5 42 205541.0 43 207380.0 44 207411.0 45 204942.0 46 201288.0 47 194554.5 48 185522.0 49 178238.0 50 172878.5 51 164934.0 52 153366.0 53 142272.0 54 132390.5 55 123274.0 56 113180.5 57 100829.5 58 89503.5 59 79380.5 60 68236.0 61 56336.0 62 46657.5 63 38411.0 64 30509.5 65 24149.0 66 18892.5 67 14221.0 68 10692.0 69 8119.5 70 5912.5 71 4124.5 72 2937.5 73 2109.0 74 1559.0 75 1111.0 76 769.5 77 529.0 78 330.0 79 218.5 80 137.0 81 63.5 82 33.0 83 20.5 84 11.5 85 8.0 86 5.5 87 2.0 88 0.5 89 0.5 90 1.0 91 1.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04195851274721816 2 0.0 3 0.0 4 7.278146183385631E-5 5 0.0 6 0.0 7 0.0 8 0.0011099172929663085 9 0.0 10-11 0.0 12-13 0.04471511061417546 14-15 6.368377910462426E-5 16-17 0.0 18-19 9.097682729232037E-5 20-21 8.187914456308834E-5 22-23 0.0 24-25 1.2736755820924852E-4 26-27 1.0007451002155241E-4 28-29 4.184934055446737E-4 30-31 9.097682729232038E-6 32-33 2.45637433689265E-4 34-35 1.1826987548001647E-4 36-37 1.0007451002155241E-4 38-39 3.002235300646572E-4 40-41 1.6375828912617668E-4 42-43 5.185679155662261E-4 44-45 2.820281646061932E-4 46-47 1.2736755820924852E-4 48-49 9.006705901939717E-4 50-51 5.094702328369941E-4 52-53 6.823262046924028E-4 54-55 4.82177184649298E-4 56-57 5.822516946708505E-4 58-59 1.6375828912617668E-4 60-61 1.8195365458464075E-4 62-63 9.006705901939717E-4 64-65 5.185679155662261E-4 66-67 0.0015648014294279105 68-69 0.001710364353095623 70-71 4.82177184649298E-4 72-73 0.0010280381484032203 74-75 0.0010371358311324522 76-77 8.460844938185795E-4 78-79 0.0034753148025666383 80-81 0.0027384025014988433 82-83 0.0012281871684463252 84-85 0.0029749422524588762 86-87 0.00142833618848943 88-89 0.0020378809313479763 90-91 0.002674718722394219 92-93 0.002620132626018827 94-95 0.0037300499189851354 96-97 0.0030295283488342684 98-99 0.0027747932324157713 100 0.0014010431403017338 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5495905.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.23311857725607 #Duplication Level Percentage of deduplicated Percentage of total 1 76.07148864749196 46.58094484700265 2 14.245670714353285 17.44613688129078 3 4.437131776061374 8.15098248559431 4 1.969260810492225 4.823359228736552 5 0.9707947394226525 2.9722394696621848 6 0.5725578790915719 2.1035702701653882 7 0.3622481593289658 1.5527109146208322 8 0.25057769844023087 1.2274923137125258 9 0.17988787965872696 0.9913586279178599 >10 0.8853348407995127 9.333665735397267 >50 0.03878447012800166 1.6255250421386875 >100 0.01528232932605635 1.5374396841365672 >500 5.345779276181267E-4 0.23184623883282163 >1k 2.9698498528086674E-4 0.3343006790230808 >5k 5.939699702864438E-5 0.2804539558583096 >10k+ 8.909549554296658E-5 0.807973625910044 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 17772 0.3233680349278235 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13578 0.24705667219502522 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13080 0.2379953801967101 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 8215 0.14947492724128236 RNA PCR Primer, Index 46 (95% over 21bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7207 0.13113399885915059 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.8195365458464073E-5 0.0 5 0.0 0.0 0.0 1.8195365458464073E-5 0.0 6 0.0 0.0 0.0 1.8195365458464073E-5 0.0 7 0.0 0.0 0.0 5.458609637539223E-5 0.0 8 0.0 0.0 0.0 9.097682729232037E-5 0.0 9 0.0 0.0 0.0 1.6375828912617668E-4 0.0 10-11 0.0 0.0 0.0 2.0014902004310481E-4 0.0 12-13 0.0 9.097682729232037E-6 0.0 3.7300499189851354E-4 0.0 14-15 0.0 2.729304818769611E-5 0.0 4.0029804008620963E-4 0.0 16-17 0.0 3.639073091692815E-5 0.0 4.7307950192006595E-4 0.0 18-19 0.0 3.639073091692815E-5 0.0 6.186424255877785E-4 0.0 20-21 0.0 3.639073091692815E-5 0.0 9.461590038401318E-4 0.0 22-23 0.0 3.639073091692815E-5 0.0 0.002201639220474153 0.0 24-25 0.0 3.639073091692815E-5 0.0 0.004930944039243764 0.0 26-27 0.0 3.639073091692815E-5 0.0 0.01044413977315838 0.0 28-29 0.0 3.639073091692815E-5 0.0 0.03145068919495515 0.0 30-31 0.0 3.639073091692815E-5 0.0 0.09381530430384077 0.0 32-33 0.0 3.639073091692815E-5 0.0 0.23222744934637699 0.0 34-35 0.0 3.639073091692815E-5 0.0 0.4442398476684004 0.0 36-37 0.0 3.639073091692815E-5 0.0 0.766079471897713 0.0 38-39 0.0 3.639073091692815E-5 0.0 1.1715358980913972 0.0 40-41 0.0 5.458609637539223E-5 0.0 1.5662934493955045 0.0 42-43 0.0 5.458609637539223E-5 0.0 1.9513710662757089 0.0 44-45 0.0 5.458609637539223E-5 0.0 2.4033075535330397 0.0 46-47 0.0 5.458609637539223E-5 0.0 2.931737357177753 0.0 48-49 0.0 5.458609637539223E-5 0.0 3.455772980064248 0.0 50-51 0.0 5.458609637539223E-5 0.0 3.95116909771912 0.0 52-53 0.0 5.458609637539223E-5 0.0 4.411575527597366 0.0 54-55 0.0 5.458609637539223E-5 0.0 4.905388648457351 0.0 56-57 0.0 5.458609637539223E-5 0.0 5.455971309547746 0.0 58-59 0.0 5.458609637539223E-5 0.0 6.032382291906428 0.0 60-61 0.0 5.458609637539223E-5 0.0 6.599140632889397 0.0 62-63 0.0 5.458609637539223E-5 0.0 7.1298084664855015 0.0 64-65 0.0 5.458609637539223E-5 0.0 7.669810522561798 0.0 66-67 0.0 5.458609637539223E-5 0.0 8.260513964488105 0.0 68-69 0.0 5.458609637539223E-5 0.0 8.882295090617468 0.0 70-71 0.0 7.27814618338563E-5 0.0 9.508106490195882 0.0 72-73 0.0 7.27814618338563E-5 0.0 10.114539825561032 0.0 74-75 0.0 7.27814618338563E-5 0.0 10.70545251419011 0.0 76-77 0.0 7.27814618338563E-5 0.0 11.313678093052918 0.0 78-79 0.0 7.27814618338563E-5 0.0 11.963052490900044 0.0 80-81 0.0 7.27814618338563E-5 0.0 12.636581236393278 0.0 82-83 0.0 7.27814618338563E-5 0.0 13.290586354749582 0.0 84-85 0.0 7.27814618338563E-5 0.0 13.917380304062752 0.0 86-87 0.0 7.27814618338563E-5 0.0 14.553271936105155 0.0 88 0.0 7.27814618338563E-5 0.0 15.05113352577965 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9075 0.0 57.759327 1 GTATCAA 16180 0.0 40.269726 1 ATCAACG 19820 0.0 32.390266 3 TCAACGC 19855 0.0 32.38051 4 CAACGCA 20710 0.0 31.0437 5 TATCAAC 20965 0.0 30.755777 2 AACGCAG 21390 0.0 30.036087 6 GTGGTAT 4315 0.0 27.236614 1 TGGTATC 4105 0.0 26.78984 2 ACGCAGA 24580 0.0 26.04232 7 CGCAGAG 24815 0.0 25.681978 8 GCAGAGT 27070 0.0 23.090652 9 GTACATG 12445 0.0 23.080236 1 TACATGG 12575 0.0 21.713789 2 GAGTACT 19980 0.0 21.683596 12-13 CAGAGTA 26425 0.0 21.02898 10-11 TTTCGCG 300 1.9099389E-10 20.380817 9 AGAGTAC 24980 0.0 20.155508 10-11 ACATGGG 12850 0.0 19.969028 3 GTACTTT 21885 0.0 19.61668 14-15 >>END_MODULE