FastQCFastQC Report
Wed 25 May 2016
SRR2049360_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049360_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5974127
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63200.048.7254831
GTATCAA106850.035.992391
TCAACGC115900.032.2064974
ATCAACG118700.031.5259953
CAACGCA124100.030.0405485
TATCAAC130650.028.9662932
AACGCAG130100.028.6850176
ACGCAGA145650.025.3643637
CGCAGAG147900.024.8511718
GTACATG164250.024.0725731
TACATGG165300.022.666892
GCAGAGT167900.020.8831679
ACATGGG175250.020.6288383
GTGGTAT35250.019.8727991
CATGGGG124650.018.6312124
CAGAGTA163450.018.50426910-11
TGGTATC34550.018.0971452
AGAGTAC148600.017.79147510-11
GAGTACA128100.017.0661581
GAGTACT79500.016.7607812-13
Produced by FastQC (version 0.11.5)