FastQCFastQC Report
Wed 25 May 2016
SRR2049360_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049360_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5974127
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT183870.3077771865244913No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166810.2792207129175526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159130.2663652781402203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92620.15503520430683845No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT88590.14828944881821227RNA PCR Primer, Index 46 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108900.059.7517471
GTATCAA198500.041.87591
GTGGTAT41600.033.340421
ATCAACG252950.032.513253
TCAACGC253600.032.4855044
CAACGCA259800.031.692175
TATCAAC263600.031.3244422
AACGCAG269200.030.664346
TGGTATC44650.030.6286982
ACGCAGA307700.026.751357
CGCAGAG310650.026.3158388
GCAGAGT333700.024.2441779
GTACATG161500.023.0274751
GAGTACT245750.021.83887512-13
TACATGG163050.021.6459242
CAGAGTA327750.021.5126610-11
AGAGTAC315750.021.1012610-11
GTACTTT263450.020.15744214-15
ACATGGG171550.020.1076983
AGTACTT254350.020.08379712-13
Produced by FastQC (version 0.11.5)