##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049360_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5974127 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.789897670404397 31.0 31.0 34.0 30.0 34.0 2 32.006564306383176 33.0 31.0 34.0 30.0 34.0 3 31.965326816788462 33.0 31.0 34.0 30.0 34.0 4 35.676514074776115 37.0 35.0 37.0 33.0 37.0 5 35.771103962135385 37.0 35.0 37.0 35.0 37.0 6 35.81230680231605 37.0 35.0 37.0 35.0 37.0 7 35.75938409076338 37.0 35.0 37.0 33.0 37.0 8 35.75923645412961 37.0 35.0 37.0 33.0 37.0 9 37.501443474502636 39.0 37.0 39.0 35.0 39.0 10-11 37.46155915332901 39.0 37.0 39.0 34.5 39.0 12-13 37.40415227195538 39.0 37.0 39.0 34.0 39.0 14-15 38.67064635552609 40.0 38.0 41.0 34.0 41.0 16-17 38.70015577171359 40.0 38.0 41.0 34.0 41.0 18-19 38.77939270457424 40.0 38.0 41.0 35.0 41.0 20-21 38.815551042018356 40.0 38.0 41.0 35.0 41.0 22-23 38.78227429714835 40.0 38.0 41.0 34.5 41.0 24-25 38.75390638665699 40.0 38.0 41.0 34.0 41.0 26-27 38.63245943716964 40.0 38.0 41.0 34.0 41.0 28-29 38.54202371325551 40.0 38.0 41.0 34.0 41.0 30-31 38.42325347285051 40.0 38.0 41.0 34.0 41.0 32-33 38.260143197491445 40.0 38.0 41.0 34.0 41.0 34-35 38.137416395734476 40.0 38.0 41.0 33.5 41.0 36-37 37.999873705396624 40.0 37.5 41.0 33.0 41.0 38-39 37.87645249255665 40.0 37.0 41.0 33.0 41.0 40-41 37.765543986594196 40.0 37.0 41.0 33.0 41.0 42-43 37.61628025316502 40.0 37.0 41.0 33.0 41.0 44-45 37.41739596429738 40.0 37.0 41.0 32.5 41.0 46-47 37.16010406206631 40.0 37.0 41.0 31.5 41.0 48-49 36.929349761061324 40.0 37.0 41.0 31.0 41.0 50-51 36.756740273516115 40.0 36.0 41.0 31.0 41.0 52-53 36.61832825114029 40.0 36.0 41.0 31.0 41.0 54-55 36.473070542357064 39.0 35.5 41.0 30.0 41.0 56-57 36.32426043169153 39.0 35.0 41.0 30.0 41.0 58-59 36.13175280672807 39.0 35.0 41.0 30.0 41.0 60-61 35.94429127469168 39.0 35.0 41.0 30.0 41.0 62-63 35.73403419445218 38.5 35.0 41.0 29.0 41.0 64-65 35.48395631696481 38.0 35.0 40.0 29.0 41.0 66-67 35.17267033325538 37.5 35.0 40.0 28.5 41.0 68-69 34.832341528728804 37.0 34.0 40.0 28.0 41.0 70-71 34.46796778843168 36.5 34.0 39.5 28.0 41.0 72-73 34.1632012509945 36.0 34.0 39.0 28.0 41.0 74-75 33.84885532229228 35.5 34.0 39.0 27.5 40.5 76-77 33.51162454363625 35.0 34.0 38.0 27.0 40.0 78-79 33.166957280955025 35.0 34.0 37.0 26.0 39.0 80-81 32.834805989226545 35.0 33.0 37.0 26.0 39.0 82-83 32.52901478324783 35.0 33.0 36.0 26.0 39.0 84-85 32.25400380674867 35.0 33.0 36.0 26.0 37.0 86-87 32.01220245234157 35.0 33.0 35.5 25.5 37.0 88-89 31.771008467011164 35.0 33.0 35.0 25.0 36.5 90-91 31.56475054849018 35.0 33.0 35.0 24.5 36.0 92-93 31.371055804471517 35.0 33.0 35.0 24.0 36.0 94-95 31.20086399234566 35.0 33.0 35.0 23.5 36.0 96-97 30.98586990199572 35.0 32.5 35.0 21.5 35.5 98-99 30.816915006996002 35.0 32.5 35.0 20.0 35.0 100 30.69156849193196 35.0 32.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2134230916937625 1101 2 0.2222519072622049 1101 3 0.39383250288961236 1101 4 0.2165656836941423 1101 5 0.19391892736027927 1101 6 0.16958050801661528 1101 7 0.08663113733113192 1101 8 0.17782048615182333 1101 9 0.28202490875758457 1101 10-11 0.24701995825126488 1101 12-13 0.18941000087696835 1101 14-15 0.33507806862679956 1101 16-17 0.19615250208794066 1101 18-19 0.306464624220979 1101 20-21 0.3117609524053009 1101 22-23 0.3222575808889445 1101 24-25 0.3029559801793127 1101 26-27 0.3261666122838065 1101 28-29 0.35159582208230233 1101 30-31 0.2833356477595359 1101 32-33 0.34954084153302745 1101 34-35 0.39484281075338856 1101 36-37 0.4149587597007738 1101 38-39 0.3690770172979825 1101 40-41 0.42900670220032566 1101 42-43 0.418729355795044 1101 44-45 0.47644652729893266 1101 46-47 0.3916227345219099 1101 48-49 0.4780871621798539 1101 50-51 0.4511959489954762 1101 52-53 0.3978215770446383 1101 54-55 0.4111820851074697 1101 56-57 0.46336477153116107 1101 58-59 0.46724723189993256 1101 60-61 0.4670397935671531 1101 62-63 0.3372111721508091 1101 64-65 0.27696029822578083 1101 66-67 0.2951820726762193 1101 68-69 0.3921092554187595 1101 70-71 0.41157339519293856 1101 72-73 0.4002871811524855 1101 74-75 0.4239133015050456 1101 76-77 0.3730936316050233 1101 78-79 0.39462536295420136 1101 80-81 0.46938415517342236 1101 82-83 0.39729293601684645 1101 84-85 0.466462997829332 1101 86-87 0.4430917387406055 1101 88-89 0.4451151589602169 1101 90-91 0.521811020690901 1101 92-93 0.5199128334856908 1101 94-95 0.528279719425484 1101 96-97 0.5179887916878059 1101 98-99 0.5689745482670006 1101 100 0.5610117601715245 1102 1 0.20655665137996948 1102 2 0.18359681860879817 1102 3 0.3701386716564521 1102 4 0.21303415926244895 1102 5 0.18714202308606076 1102 6 0.19017347345862845 1102 7 0.18319518224986098 1102 8 0.16193575407827154 1102 9 0.1814310211796908 1102 10-11 0.20424768295792717 1102 12-13 0.20750416639761937 1102 14-15 0.2839796331079967 1102 16-17 0.2019013602055253 1102 18-19 0.27327874435748356 1102 20-21 0.1944518881935906 1102 22-23 0.21852687922665837 1102 24-25 0.17027372067747137 1102 26-27 0.2765921137014402 1102 28-29 0.24633999918334837 1102 30-31 0.19919265396713826 1102 32-33 0.23949160392004387 1102 34-35 0.22338203791789368 1102 36-37 0.26476994620056615 1102 38-39 0.24789690964023947 1102 40-41 0.23534799436956177 1102 42-43 0.2614869531214623 1102 44-45 0.31773977049539326 1102 46-47 0.29091694160634773 1102 48-49 0.2456901189523535 1102 50-51 0.2983393263687333 1102 52-53 0.34008609501701415 1102 54-55 0.22913654439430786 1102 56-57 0.2572254297675869 1102 58-59 0.2508454578858732 1102 60-61 0.28749929058028556 1102 62-63 0.2941076605888284 1102 64-65 0.2079507561643723 1102 66-67 0.23527336295131107 1102 68-69 0.28485716575778497 1102 70-71 0.28165533655788977 1102 72-73 0.25169745248390996 1102 74-75 0.21582282879433023 1102 76-77 0.23183350026598504 1102 78-79 0.2986143126827727 1102 80-81 0.2969448340460872 1102 82-83 0.3362829962659575 1102 84-85 0.33120726128866806 1102 86-87 0.31393820107178527 1102 88-89 0.3366006714596601 1102 90-91 0.3729832697442639 1102 92-93 0.4167696992102705 1102 94-95 0.4389807973549509 1102 96-97 0.4552993597401347 1102 98-99 0.42921483704559193 1102 100 0.48366945228462654 1103 1 0.10641307596758764 1103 2 0.15673245151558746 1103 3 0.28171396907776014 1103 4 0.16547384237372142 1103 5 0.15230311326173052 1103 6 0.10705400282375166 1103 7 0.11273080433672078 1103 8 0.12385158346825165 1103 9 0.1503769316684327 1103 10-11 0.1305926510983042 1103 12-13 0.1736918820565947 1103 14-15 0.21713595913280415 1103 16-17 0.2238824937961752 1103 18-19 0.1620139916351775 1103 20-21 0.21251741132879687 1103 22-23 0.1826560607495935 1103 24-25 0.2223135101283802 1103 26-27 0.20567184053199838 1103 28-29 0.26604681286582377 1103 30-31 0.1986649710046393 1103 32-33 0.21592630140999347 1103 34-35 0.2175116125086376 1103 36-37 0.2427251609846408 1103 38-39 0.20990394766722886 1103 40-41 0.26904360519692716 1103 42-43 0.3095262421505609 1103 44-45 0.2427074163658176 1103 46-47 0.23338407183943133 1103 48-49 0.18424639358248385 1103 50-51 0.2437023519595698 1103 52-53 0.24515381679601944 1103 54-55 0.21802212954123945 1103 56-57 0.2174650996920633 1103 58-59 0.294454154236341 1103 60-61 0.26213447716492055 1103 62-63 0.26792930247953706 1103 64-65 0.28691664126008476 1103 66-67 0.22086759694246183 1103 68-69 0.23537394425858338 1103 70-71 0.2372016078237067 1103 72-73 0.26262015168475017 1103 74-75 0.21770989238340377 1103 76-77 0.2234777662942733 1103 78-79 0.2558630324651574 1103 80-81 0.26624127226006067 1103 82-83 0.22366805563186887 1103 84-85 0.23255890735520524 1103 86-87 0.221846513990954 1103 88-89 0.24551139181347637 1103 90-91 0.2778927288998716 1103 92-93 0.3150282609033219 1103 94-95 0.2563557891132078 1103 96-97 0.3077656488926479 1103 98-99 0.30797370643042754 1103 100 0.40051232321806296 1104 1 0.16971859345252938 1104 2 0.18455348399063354 1104 3 0.27209295798705924 1104 4 0.1554266834135163 1104 5 0.1483721198941197 1104 6 0.11331783379670668 1104 7 0.11816194188666174 1104 8 0.13675193242187333 1104 9 0.15512336518094116 1104 10-11 0.1479582780322133 1104 12-13 0.15451239288323393 1104 14-15 0.2083098948566473 1104 16-17 0.2057943757157048 1104 18-19 0.16988768305908053 1104 20-21 0.2107550659197912 1104 22-23 0.2032874983283719 1104 24-25 0.20839590627659987 1104 26-27 0.2372461900105094 1104 28-29 0.227073196611137 1104 30-31 0.23101810753720287 1104 32-33 0.21807299801474755 1104 34-35 0.17843872915376124 1104 36-37 0.1873266264602691 1104 38-39 0.2315981223773207 1104 40-41 0.1988842027273563 1104 42-43 0.1941483467588867 1104 44-45 0.18567395148636479 1104 46-47 0.214164892026254 1104 48-49 0.18535829850639374 1104 50-51 0.1471634567525797 1104 52-53 0.14721072345335529 1104 54-55 0.1364333618303064 1104 56-57 0.14704961159439733 1104 58-59 0.22779741022962696 1104 60-61 0.1624731031235669 1104 62-63 0.1795088441417576 1104 64-65 0.12517064191457905 1104 66-67 0.18887264818388871 1104 68-69 0.140508860033421 1104 70-71 0.20547574540280777 1104 72-73 0.24592614707739813 1104 74-75 0.2019141517560641 1104 76-77 0.14737533852314044 1104 78-79 0.18237532684333502 1104 80-81 0.16750614814985454 1104 82-83 0.16029409204357847 1104 84-85 0.15513607729918277 1104 86-87 0.17820390147203824 1104 88-89 0.22443015398392774 1104 90-91 0.1638292821971099 1104 92-93 0.24400635653022107 1104 94-95 0.2346567543247673 1104 96-97 0.22273529770338385 1104 98-99 0.24830571458239703 1104 100 0.2907844714050931 1105 1 0.1841192003543597 1105 2 0.1410052794946992 1105 3 0.309481548336084 1105 4 0.19938770005162354 1105 5 0.13653215794179374 1105 6 0.12325698852824019 1105 7 0.13488304361509051 1105 8 0.12010441883249712 1105 9 0.15950574951935437 1105 10-11 0.16615972394738066 1105 12-13 0.1534028067809814 1105 14-15 0.2506114563106294 1105 16-17 0.2619012995143706 1105 18-19 0.20804619609791075 1105 20-21 0.2014407641799849 1105 22-23 0.18741551635966403 1105 24-25 0.20569471496036584 1105 26-27 0.19753856522471835 1105 28-29 0.2000569410506685 1105 30-31 0.17500405419291098 1105 32-33 0.21220234719783093 1105 34-35 0.24963813569268467 1105 36-37 0.21268625714964884 1105 38-39 0.22032463659479617 1105 40-41 0.2356534327012838 1105 42-43 0.24054183006003882 1105 44-45 0.2700607686894969 1105 46-47 0.2795142612153896 1105 48-49 0.2762633266415122 1105 50-51 0.34242023981101966 1105 52-53 0.32110399351174124 1105 54-55 0.30783974308769047 1105 56-57 0.31885535131415566 1105 58-59 0.26874821447793806 1105 60-61 0.2878000624368511 1105 62-63 0.3607683592923294 1105 64-65 0.3113706687960942 1105 66-67 0.3140838231385885 1105 68-69 0.3354652034240644 1105 70-71 0.32617270029614076 1105 72-73 0.34757280769572674 1105 74-75 0.3321760526408255 1105 76-77 0.29221332571823666 1105 78-79 0.3080851667691036 1105 80-81 0.2877205411317121 1105 82-83 0.31002164533488497 1105 84-85 0.36759161295207576 1105 86-87 0.36646037694394806 1105 88-89 0.38542066015762444 1105 90-91 0.3854055210083267 1105 92-93 0.4480468028667133 1105 94-95 0.46563995854929985 1105 96-97 0.46446752682691894 1105 98-99 0.4939931187847044 1105 100 0.5409836530698335 1106 1 0.1730137801158662 1106 2 0.17223373325659708 1106 3 0.25146308285495067 1106 4 0.19780484087797134 1106 5 0.1522354165778168 1106 6 0.1396877866290609 1106 7 0.1502369509900916 1106 8 0.12137587591586652 1106 9 0.1991465604953504 1106 10-11 0.15673000677077198 1106 12-13 0.18371895559189966 1106 14-15 0.23649751902882343 1106 16-17 0.22380780264462175 1106 18-19 0.22222612632357652 1106 20-21 0.18742864774514345 1106 22-23 0.21137981511776616 1106 24-25 0.16559623280422642 1106 26-27 0.24738965223605192 1106 28-29 0.2574458142947833 1106 30-31 0.3229468980196515 1106 32-33 0.220641124954156 1106 34-35 0.22832014599037365 1106 36-37 0.2566393408579515 1106 38-39 0.26566771013330026 1106 40-41 0.2665191804389053 1106 42-43 0.26480132319389327 1106 44-45 0.28430360526088805 1106 46-47 0.35868395401894304 1106 48-49 0.32247429992830234 1106 50-51 0.30643846180751666 1106 52-53 0.32196375251113807 1106 54-55 0.32476897759905654 1106 56-57 0.2924094522471421 1106 58-59 0.32467611843186006 1106 60-61 0.33074156586838654 1106 62-63 0.31964315900162177 1106 64-65 0.2778738543867192 1106 66-67 0.27491037353929215 1106 68-69 0.31989916313595756 1106 70-71 0.3535129503966772 1106 72-73 0.3951698541533304 1106 74-75 0.35389547841059965 1106 76-77 0.3162771641920088 1106 78-79 0.3266726976504586 1106 80-81 0.30770256876844826 1106 82-83 0.31186352665948647 1106 84-85 0.32719943667904516 1106 86-87 0.37213974211310585 1106 88-89 0.36140909094834583 1106 90-91 0.3749117882782116 1106 92-93 0.4119088503757311 1106 94-95 0.39511337383939704 1106 96-97 0.44884314245567936 1106 98-99 0.4719917242817999 1106 100 0.4729241518198215 1107 1 0.14379292268600352 1107 2 0.11770262225730477 1107 3 0.263747594638577 1107 4 0.1588151501887154 1107 5 0.14513169560105865 1107 6 0.16027269149630996 1107 7 0.12170745676980488 1107 8 0.1298906962076103 1107 9 0.1284505276593393 1107 10-11 0.1359793573004282 1107 12-13 0.11452909031412162 1107 14-15 0.20427738824977126 1107 16-17 0.13751781084562964 1107 18-19 0.14331196871032148 1107 20-21 0.12972181945072236 1107 22-23 0.12318363244335728 1107 24-25 0.12840788776144052 1107 26-27 0.19340076227180703 1107 28-29 0.22481164632066708 1107 30-31 0.23612818730403262 1107 32-33 0.23094747820251627 1107 34-35 0.1267994075695711 1107 36-37 0.17566566685881213 1107 38-39 0.19947636841762062 1107 40-41 0.26912240553265576 1107 42-43 0.2807677954284671 1107 44-45 0.2228362792925438 1107 46-47 0.24310788521410132 1107 48-49 0.23273563262701913 1107 50-51 0.28304105198631646 1107 52-53 0.1973340104707475 1107 54-55 0.21171695399033297 1107 56-57 0.23819502513423174 1107 58-59 0.2689381358409122 1107 60-61 0.26334736927763913 1107 62-63 0.267828830137006 1107 64-65 0.19883578552980197 1107 66-67 0.2040462396264573 1107 68-69 0.2295513473752635 1107 70-71 0.2274032059874287 1107 72-73 0.21973227126317596 1107 74-75 0.19222289762961253 1107 76-77 0.1308032109150261 1107 78-79 0.13612654773102406 1107 80-81 0.20979335246639153 1107 82-83 0.1911795987196001 1107 84-85 0.21900346275487692 1107 86-87 0.19143479180922895 1107 88-89 0.16446074109169118 1107 90-91 0.21092939313641423 1107 92-93 0.2654777572142315 1107 94-95 0.28393565135174015 1107 96-97 0.2871534772401425 1107 98-99 0.258719795258596 1107 100 0.290411738558884 1108 1 0.11160482594617349 1108 2 0.0867862320615913 1108 3 0.1780610932359714 1108 4 0.11415614761553883 1108 5 0.09841498577103636 1108 6 0.08883704104729873 1108 7 0.06548315685321171 1108 8 0.021851995439057248 1108 9 0.15812922300113286 1108 10-11 0.14272978027359073 1108 12-13 0.10991951428496094 1108 14-15 0.19007686987382044 1108 16-17 0.11202288406713024 1108 18-19 0.10271726863864217 1108 20-21 0.10940840833895038 1108 22-23 0.030866740990909136 1108 24-25 0.05332767213487699 1108 26-27 0.11437946566303481 1108 28-29 0.16144349792841695 1108 30-31 0.1789834224022826 1108 32-33 0.14953251441929893 1108 34-35 0.17952780173784788 1108 36-37 0.16731298622744362 1108 38-39 0.17290407411370978 1108 40-41 0.19297625408766805 1108 42-43 0.22858422602251238 1108 44-45 0.1808664676908478 1108 46-47 0.2102464042468526 1108 48-49 0.13621895877381007 1108 50-51 0.1648536880094582 1108 52-53 0.11673920080583855 1108 54-55 0.12499524538661433 1108 56-57 0.16938642346441668 1108 58-59 0.18932914556863523 1108 60-61 0.18784767613865938 1108 62-63 0.1977495139306953 1108 64-65 0.15621247709176345 1108 66-67 0.1564921100215173 1108 68-69 0.06886278637586685 1108 70-71 0.1645016082316033 1108 72-73 0.22760829222798407 1108 74-75 0.24235813661275074 1108 76-77 0.13948375006833658 1108 78-79 0.15220276358282092 1108 80-81 0.20231798448824634 1108 82-83 0.19606799975068157 1108 84-85 0.2361605417780126 1108 86-87 0.28427095201789143 1108 88-89 0.24156814793083825 1108 90-91 0.26299044959997886 1108 92-93 0.26765844979805564 1108 94-95 0.2165302444755568 1108 96-97 0.28029724011970814 1108 98-99 0.2987117562177133 1108 100 0.23674913301399414 1109 1 0.011475983468987039 1109 2 0.012862935879375925 1109 3 0.12992781861824554 1109 4 0.04753842371216166 1109 5 0.018424996171056307 1109 6 -0.07499375772614059 1109 7 0.051994271082506316 1109 8 0.015831289130673554 1109 9 0.0629571207505748 1109 10-11 0.08100500392577459 1109 12-13 0.055690116810374946 1109 14-15 0.08245636331125183 1109 16-17 0.12432288751500664 1109 18-19 0.04749426073782104 1109 20-21 0.04893471823438489 1109 22-23 0.1099911907978921 1109 24-25 0.09294098548161145 1109 26-27 0.05707416607157256 1109 28-29 0.08441316589409098 1109 30-31 0.11323952868158926 1109 32-33 0.15026243845783682 1109 34-35 0.08835719617128035 1109 36-37 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2215 12-13 -0.34587858928054516 2215 14-15 -0.39981617646672873 2215 16-17 -0.44970879805820374 2215 18-19 -0.4335352383240263 2215 20-21 -0.4181213164540125 2215 22-23 -0.404739138214552 2215 24-25 -0.39454197301525795 2215 26-27 -0.45167559297872373 2215 28-29 -0.40546637843148403 2215 30-31 -0.4284070964691864 2215 32-33 -0.3941875638017507 2215 34-35 -0.44527495334845213 2215 36-37 -0.41366624709892363 2215 38-39 -0.4974148426895084 2215 40-41 -0.4241619230325284 2215 42-43 -0.42557189674959517 2215 44-45 -0.4621234491118713 2215 46-47 -0.44795787391609565 2215 48-49 -0.4254509762170926 2215 50-51 -0.447713678714905 2215 52-53 -0.43136885953791193 2215 54-55 -0.32279100695463114 2215 56-57 -0.41077812467415953 2215 58-59 -0.39554356460899953 2215 60-61 -0.447588759898764 2215 62-63 -0.35139794270047986 2215 64-65 -0.3343616291635172 2215 66-67 -0.33881383387654296 2215 68-69 -0.35326659949347317 2215 70-71 -0.3960018631131703 2215 72-73 -0.33196445654316165 2215 74-75 -0.30738442071369576 2215 76-77 -0.23892609613262294 2215 78-79 -0.27640415768061644 2215 80-81 -0.31720147366811347 2215 82-83 -0.24880393698247616 2215 84-85 -0.34716988820409256 2215 86-87 -0.303315203980425 2215 88-89 -0.3723704878042362 2215 90-91 -0.39758125429614566 2215 92-93 -0.3202956205249876 2215 94-95 -0.4406653452586653 2215 96-97 -0.4187844281606843 2215 98-99 -0.3964211092818246 2215 100 -0.35201308736738923 2216 1 -0.40945775455185895 2216 2 -0.43944182598331594 2216 3 -0.49916391645922076 2216 4 -0.27595390519589813 2216 5 -0.4766420687878892 2216 6 -0.3221900891792444 2216 7 -0.5759163032221295 2216 8 -0.3789238349012507 2216 9 -0.612440631147912 2216 10-11 -0.38075647015748615 2216 12-13 -0.434218092206514 2216 14-15 -0.6766333464353238 2216 16-17 -0.498867471973071 2216 18-19 -0.4245165236363846 2216 20-21 -0.4416793377933814 2216 22-23 -0.48561985773710603 2216 24-25 -0.4194475812178595 2216 26-27 -0.582935727598823 2216 28-29 -0.6238858627727808 2216 30-31 -0.7539499689232798 2216 32-33 -0.7132385376822299 2216 34-35 -0.744112718251003 2216 36-37 -0.8096214736095391 2216 38-39 -0.6687580473710568 2216 40-41 -0.6302416391410688 2216 42-43 -0.7550107201074141 2216 44-45 -0.9396898797523008 2216 46-47 -0.904365725676918 2216 48-49 -0.9679997824355553 2216 50-51 -0.8313347797116606 2216 52-53 -0.8030905223646698 2216 54-55 -0.7596150603802982 2216 56-57 -0.8496888621412211 2216 58-59 -0.860715647735816 2216 60-61 -0.7324870292669772 2216 62-63 -0.6846907430531743 2216 64-65 -0.7436828727791678 2216 66-67 -0.6206305847660119 2216 68-69 -0.6426477615239818 2216 70-71 -0.6980654153069992 2216 72-73 -0.655472980348506 2216 74-75 -0.6450936880379956 2216 76-77 -0.5938133506496044 2216 78-79 -0.7944766643083341 2216 80-81 -0.9257784063653247 2216 82-83 -0.7875451288962907 2216 84-85 -0.8942787042428755 2216 86-87 -0.7565204002975463 2216 88-89 -1.09118189362 2216 90-91 -0.8750190903431552 2216 92-93 -1.0016061766330822 2216 94-95 -0.9804678632972177 2216 96-97 -1.0357994034746127 2216 98-99 -1.148154875905572 2216 100 -1.0689153732955035 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 322.0 3 51.0 4 57.0 5 105.0 6 301.0 7 911.0 8 2162.0 9 3728.0 10 5813.0 11 8273.0 12 11510.0 13 15707.0 14 19378.0 15 24588.0 16 30628.0 17 40547.0 18 40494.0 19 23488.0 20 18249.0 21 18629.0 22 20304.0 23 23574.0 24 28924.0 25 34788.0 26 40587.0 27 44827.0 28 57585.0 29 74200.0 30 96490.0 31 124441.0 32 159559.0 33 208029.0 34 282628.0 35 407528.0 36 656073.0 37 1178610.0 38 1616197.0 39 652758.0 40 2084.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.82560188729671 20.88525308303342 15.170564038879277 23.118580990790587 2 15.347748717092891 20.555538909701788 40.44395105761896 23.65276131558636 3 17.590017754895403 25.612428393303322 31.429914362382988 25.367639489418288 4 12.457462368528798 17.810098287246227 38.19473388725573 31.53770545696924 5 12.537078639272314 37.73127019228081 35.82103293083659 13.910618237610281 6 29.99427866101251 38.586598273956724 17.2428402079233 14.176282857107466 7 25.860698977440556 33.4033742503298 22.40929930013205 18.3266274720976 8 24.437787910439766 36.5416767100541 20.353209197064654 18.667326182441478 9 24.42862362986257 16.703327532206796 21.777357595511447 37.09069124241918 10-11 23.588425555733917 27.203949631469165 28.650160935648007 20.557463877148912 12-13 24.393365014227932 24.65370041417376 29.05962502893941 21.893309542658894 14-15 21.661664394504392 26.62918387039025 26.84132395432062 24.86782778078473 16-17 20.545423715535964 30.075980988683533 27.571623029042243 21.806972266738263 18-19 20.353847723435912 28.103151868219665 30.734659234948857 20.80834117339556 20-21 21.385475440116938 27.235062555212085 30.636131080338576 20.7433309243324 22-23 21.11875090703629 27.334060692047558 30.598705049290047 20.948483351626106 24-25 21.07043733142912 27.46364777802384 30.607525561509576 20.85838932903747 26-27 20.822043670677687 27.47711254020046 30.734151272077924 20.966692517043924 28-29 20.74818596197622 27.22222901078204 31.00955072251014 21.020034304731606 30-31 21.043287336088994 27.793312360669997 30.433891082980168 20.729509220260837 32-33 21.106460768266544 27.273369437446892 30.761846343175886 20.85832345111068 34-35 21.03558128950475 27.040404089437057 30.767988874685038 21.15602574637315 36-37 21.095136841051595 27.5046822787821 30.30444144000431 21.095739440161992 38-39 20.96246877461404 27.204717091119328 30.617010362471834 21.21580377179479 40-41 20.863613602575846 26.996351009776586 30.776523766644402 21.363511621003166 42-43 20.98620350893521 26.950532980621407 30.626280163268337 21.436983347175047 44-45 20.841084489844803 27.293386117778073 30.377829595830075 21.487699796547048 46-47 21.08961347184526 27.346703953060185 29.85186015309204 21.71182242200251 48-49 21.017888465708403 27.291213214167286 29.601521441067092 22.08937687905722 50-51 20.851089508026778 27.351127882036703 29.537057116010278 22.260725493926238 52-53 20.77549255643582 27.417185253579333 29.756940265516725 22.050381924468123 54-55 20.86591321952814 27.671225653749374 29.33360227214236 22.12925885458013 56-57 20.85921523458548 27.485620824992456 29.16021868431532 22.494945256106742 58-59 20.624520745074125 27.3047129810146 29.514604417690514 22.55616185622076 60-61 20.874574801001888 27.82632472142943 28.825460241848393 22.47364023572029 62-63 20.522321415867697 27.95551468255848 28.86175752139123 22.660406380182593 64-65 20.530723019074603 27.75729815500941 28.907765200606516 22.804213625309472 66-67 20.53253661828476 27.91717417579304 28.630854830754547 22.91943437516765 68-69 20.93128887397248 27.999002348915912 28.365806513439622 22.703902263671985 70-71 20.92429899424097 27.83796541568221 28.299374214839233 22.938361375237587 72-73 20.789530398728168 27.867081962301164 28.31711947867927 23.026268160291394 74-75 21.013610254932928 27.762486208084734 28.179011883289117 23.04489165369322 76-77 21.130700947243195 27.744538776141503 27.971511228604 23.153249048011297 78-79 21.176854512182626 27.79557831077638 27.983336223676396 23.044230953364593 80-81 20.97414849699513 27.742767535001033 28.079160477311056 23.203923490692784 82-83 20.935530859122085 27.622868029940147 28.142431636015218 23.29916947492255 84-85 21.065990017658336 27.442843921165043 28.001109483860674 23.490056577315944 86-87 21.162417358206127 27.56720921592477 27.950031557316095 23.320341868553 88-89 20.981647219756212 27.391323015678193 28.066590899446847 23.560438865118755 90-91 20.89872484768461 27.2066586395976 28.094337200175495 23.8002793125423 92-93 20.953793510040065 27.24789528430527 28.072021166508144 23.726290039146527 94-95 20.862000420161387 26.953344509737786 28.268591932064425 23.916063138036407 96-97 20.950651693729096 26.945947757871764 28.210290589943792 23.893109958455348 98-99 20.925267588976972 27.031338993186665 28.16244200338219 23.880951414454177 100 20.93832492195412 27.017433734233894 28.103815669436983 23.940425674375003 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 787.0 1 595.0 2 702.0 3 949.5 4 585.5 5 385.5 6 1019.0 7 3019.0 8 5254.0 9 5882.0 10 4520.5 11 2944.0 12 2743.0 13 3076.5 14 3619.0 15 4286.0 16 4982.5 17 5766.5 18 6651.0 19 7803.5 20 9325.5 21 11457.5 22 14559.0 23 19152.0 24 25433.5 25 33761.5 26 44543.5 27 57248.0 28 72717.5 29 90230.5 30 106197.5 31 120019.0 32 131759.5 33 142856.0 34 155386.0 35 166601.0 36 174829.5 37 183899.0 38 193122.0 39 199231.5 40 204770.5 41 210934.0 42 214625.5 43 217120.0 44 218125.5 45 216230.0 46 213423.0 47 208374.0 48 201817.0 49 196488.0 50 191207.5 51 182730.0 52 171548.0 53 161706.5 54 152858.0 55 143125.5 56 130207.0 57 115147.5 58 101724.5 59 90456.5 60 79206.5 61 66853.5 62 56610.0 63 47506.0 64 38258.5 65 30415.5 66 24048.5 67 18495.5 68 13970.5 69 10622.5 70 8078.0 71 6148.5 72 4420.5 73 2969.0 74 2076.0 75 1415.0 76 988.5 77 694.5 78 473.5 79 335.0 80 199.0 81 86.5 82 54.5 83 23.5 84 12.0 85 10.5 86 7.0 87 5.0 88 7.0 89 4.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.040357361000193 2 0.0 3 0.0 4 8.369423683159062E-5 5 0.0 6 1.6738847366318126E-5 7 0.0 8 7.867258262169518E-4 9 0.0 10-11 0.0 12-13 0.04351263372874396 14-15 1.4228020261370405E-4 16-17 1.6738847366318126E-5 18-19 5.858596578211343E-5 20-21 7.532481314843156E-5 22-23 0.0 24-25 1.33910778930545E-4 26-27 6.69553894652725E-5 28-29 4.01732336791635E-4 30-31 1.6738847366318126E-5 32-33 2.5945213417793094E-4 34-35 6.69553894652725E-5 36-37 1.0043308419790876E-4 38-39 3.5988521837583966E-4 40-41 1.4228020261370405E-4 42-43 5.942290815042935E-4 44-45 3.096686762768853E-4 46-47 1.4228020261370405E-4 48-49 9.038977577811787E-4 50-51 5.272736920390209E-4 52-53 6.69553894652725E-4 54-55 4.937959973063847E-4 56-57 6.025985051874525E-4 58-59 1.6738847366318125E-4 60-61 2.0923559207897658E-4 62-63 0.0011047639261769964 64-65 5.272736920390209E-4 66-67 0.001615298770849699 68-69 0.0015818210761170627 70-71 5.3564311572218E-4 72-73 0.0011633498919591096 74-75 0.001096394502493837 76-77 9.038977577811787E-4 78-79 0.003531896794293124 80-81 0.002694954425977218 82-83 0.0012554135524738593 84-85 0.0029795148312046263 86-87 0.0012805218235233365 88-89 0.002134203039205561 90-91 0.0029125594417393538 92-93 0.002694954425977218 94-95 0.0038834125889858047 96-97 0.003238966965382557 98-99 0.0030046231022541033 100 0.0012888912472064956 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5974127.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.207919454291456 #Duplication Level Percentage of deduplicated Percentage of total 1 75.04807656209985 43.68392395726207 2 14.431004284660256 16.79997470092078 3 4.74688032225473 8.289180823708932 4 1.989855991608976 4.633015091408581 5 1.0752176284135468 3.1293090555265004 6 0.6505656828288099 2.2720844919495295 7 0.4256343975200276 1.7342704909475142 8 0.2882499285485845 1.3422742898929019 9 0.21210488084580414 1.111156543812119 >10 1.0537810772149405 10.803187282960586 >50 0.056274075027127245 2.2425293865155957 >100 0.021175886473308123 2.09758568259026 >500 5.465061150590797E-4 0.20593319122742845 >1k 4.889635734442146E-4 0.4986631381049922 >5k 5.7525126212049894E-5 0.30338340502086064 >10k+ 8.628768931807484E-5 0.8535284681512332 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 18387 0.3077771865244913 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16681 0.2792207129175526 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15913 0.2663652781402203 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9262 0.15503520430683845 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 8859 0.14828944881821227 RNA PCR Primer, Index 46 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.6738847366318126E-5 0.0 4 0.0 0.0 0.0 1.6738847366318126E-5 0.0 5 0.0 0.0 0.0 1.0043308419790876E-4 0.0 6 0.0 0.0 0.0 1.0043308419790876E-4 0.0 7 0.0 0.0 0.0 1.0043308419790876E-4 0.0 8 0.0 0.0 0.0 1.0043308419790876E-4 0.0 9 0.0 0.0 0.0 2.1760501576213563E-4 0.0 10-11 0.0 0.0 0.0 2.5945213417793094E-4 0.0 12-13 0.0 0.0 0.0 3.5988521837583966E-4 0.0 14-15 0.0 0.0 0.0 4.7705714994006657E-4 0.0 16-17 0.0 0.0 0.0 5.44012539405339E-4 0.0 18-19 0.0 0.0 0.0 6.528150472864069E-4 0.0 20-21 0.0 0.0 0.0 9.122671814643377E-4 0.0 22-23 0.0 0.0 0.0 0.002402024597066651 0.0 24-25 0.0 0.0 0.0 0.005163934412509142 0.0 26-27 0.0 0.0 0.0 0.010311129977651965 0.0 28-29 0.0 0.0 0.0 0.032833249109033 0.0 30-31 0.0 0.0 0.0 0.09665010469312085 0.0 32-33 0.0 0.0 0.0 0.23485439797312646 0.0 34-35 0.0 0.0 0.0 0.4460819128886948 0.0 36-37 0.0 0.0 0.0 0.764948585793372 0.0 38-39 0.0 0.0 0.0 1.1759207663312146 0.0 40-41 0.0 0.0 0.0 1.5831099673642695 0.0 42-43 0.0 0.0 0.0 1.9759874538991218 0.0 44-45 0.0 0.0 0.0 2.4375779088727105 0.0 46-47 0.0 0.0 0.0 2.9683249117402424 0.0 48-49 0.0 0.0 0.0 3.4906271661114667 0.0 50-51 0.0 0.0 0.0 3.9940647395008506 0.0 52-53 0.0 0.0 0.0 4.459722734384455 0.0 54-55 0.0 8.369423683159063E-6 0.0 4.96165381151087 0.0 56-57 0.0 1.6738847366318126E-5 0.0 5.528590202384382 0.0 58-59 0.0 1.6738847366318126E-5 0.0 6.114734420610743 0.0 60-61 0.0 1.6738847366318126E-5 0.0 6.681319295689563 0.0 62-63 0.0 1.6738847366318126E-5 0.0 7.21695404198806 0.0 64-65 0.0 1.6738847366318126E-5 0.0 7.764481739340326 0.0 66-67 0.0 1.6738847366318126E-5 0.0 8.359472438399786 0.0 68-69 0.0 1.6738847366318126E-5 0.0 8.979897816032366 0.0 70-71 0.0 1.6738847366318126E-5 0.0 9.612701571292341 0.0 72-73 0.0 1.6738847366318126E-5 0.0 10.233352923364368 0.0 74-75 0.0 1.6738847366318126E-5 0.0 10.83163280593131 0.0 76-77 0.0 1.6738847366318126E-5 0.0 11.451037113874547 0.0 78-79 0.0 1.6738847366318126E-5 0.0 12.108095458968315 0.0 80-81 0.0 1.6738847366318126E-5 0.0 12.786403770793623 0.0 82-83 0.0 1.6738847366318126E-5 0.0 13.447035859800101 0.0 84-85 0.0 1.6738847366318126E-5 0.0 14.081145245154648 0.0 86-87 0.0 3.347769473263625E-5 0.0 14.724737187542214 0.0 88 0.0 3.347769473263625E-5 0.0 15.216867669535649 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10890 0.0 59.751747 1 GTATCAA 19850 0.0 41.8759 1 GTGGTAT 4160 0.0 33.34042 1 ATCAACG 25295 0.0 32.51325 3 TCAACGC 25360 0.0 32.485504 4 CAACGCA 25980 0.0 31.69217 5 TATCAAC 26360 0.0 31.324442 2 AACGCAG 26920 0.0 30.66434 6 TGGTATC 4465 0.0 30.628698 2 ACGCAGA 30770 0.0 26.75135 7 CGCAGAG 31065 0.0 26.315838 8 GCAGAGT 33370 0.0 24.244177 9 GTACATG 16150 0.0 23.027475 1 GAGTACT 24575 0.0 21.838875 12-13 TACATGG 16305 0.0 21.645924 2 CAGAGTA 32775 0.0 21.51266 10-11 AGAGTAC 31575 0.0 21.10126 10-11 GTACTTT 26345 0.0 20.157442 14-15 ACATGGG 17155 0.0 20.107698 3 AGTACTT 25435 0.0 20.083797 12-13 >>END_MODULE