FastQCFastQC Report
Wed 25 May 2016
SRR2049359_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049359_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4319743
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38050.048.3111421
GTATCAA69200.036.0076681
TCAACGC75700.031.361774
ATCAACG76900.031.0557793
CAACGCA80050.029.775345
AACGCAG85250.028.1748226
TATCAAC88100.027.2144372
GTACATG112900.023.9441321
ACGCAGA99500.023.7612637
CGCAGAG101250.023.1184628
TACATGG115250.022.3942472
ACATGGG117650.021.3380343
GCAGAGT116900.019.0989179
CATGGGG87900.018.2377224
AGAGTAC98700.018.09656110-11
GTATAGA21900.017.388661
CAGAGTA110850.016.77028310-11
GAGTACA86950.015.7343441
GTACTAT17900.015.4961631
GTGGTAT27050.015.4684921
Produced by FastQC (version 0.11.5)