##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049359_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4319743 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.774659510994056 31.0 31.0 34.0 28.0 34.0 2 30.868855624049857 31.0 31.0 34.0 28.0 34.0 3 31.101292368550627 31.0 31.0 34.0 28.0 34.0 4 34.740469051052344 37.0 35.0 37.0 32.0 37.0 5 34.71134532771973 37.0 35.0 37.0 32.0 37.0 6 34.685627362553745 37.0 35.0 37.0 32.0 37.0 7 34.697418341785614 37.0 35.0 37.0 32.0 37.0 8 34.65353517558799 37.0 35.0 37.0 32.0 37.0 9 36.246197979833525 39.0 37.0 39.0 32.0 39.0 10-11 36.20076576314841 39.0 37.0 39.0 32.0 39.0 12-13 36.131568938244705 39.0 37.0 39.0 32.0 39.0 14-15 37.33262337597399 40.0 38.0 41.0 32.0 41.0 16-17 37.30287102728102 40.0 38.0 41.0 31.5 41.0 18-19 37.31935476254027 40.0 37.5 41.0 32.0 41.0 20-21 37.39975236952754 40.0 38.0 41.0 32.0 41.0 22-23 37.34962079920032 40.0 38.0 41.0 32.0 41.0 24-25 37.355916659856845 40.0 38.0 41.0 31.5 41.0 26-27 37.34135433520004 40.0 38.0 41.0 32.0 41.0 28-29 37.29459414599434 40.0 37.0 41.0 31.0 41.0 30-31 37.23458941886126 40.0 37.0 41.0 31.0 41.0 32-33 37.1853184321382 40.0 37.0 41.0 31.0 41.0 34-35 37.09350938238687 40.0 37.0 41.0 31.0 41.0 36-37 37.002316688747456 40.0 37.0 41.0 30.5 41.0 38-39 36.89213270326499 40.0 37.0 41.0 30.0 41.0 40-41 36.792591133315106 40.0 37.0 41.0 30.0 41.0 42-43 36.65235524428189 40.0 36.5 41.0 30.0 41.0 44-45 36.51176308405384 40.0 36.0 41.0 30.0 41.0 46-47 36.392358179641704 40.0 36.0 41.0 30.0 41.0 48-49 36.27055116473365 40.0 36.0 41.0 30.0 41.0 50-51 36.13806955645278 39.0 35.5 41.0 29.0 41.0 52-53 35.990005886924294 39.0 35.0 41.0 29.0 41.0 54-55 35.85728479680388 39.0 35.0 41.0 29.0 41.0 56-57 35.72687356632096 39.0 35.0 41.0 28.0 41.0 58-59 35.53645922917174 39.0 35.0 40.5 28.0 41.0 60-61 35.32217518495892 38.5 35.0 40.0 28.0 41.0 62-63 35.10950304219487 38.0 34.0 40.0 28.0 41.0 64-65 34.86808717555651 38.0 34.0 40.0 27.0 41.0 66-67 34.609933276123144 37.0 34.0 40.0 27.0 41.0 68-69 34.32333601790663 37.0 34.0 40.0 26.0 41.0 70-71 34.036903468562826 36.5 34.0 39.0 26.0 41.0 72-73 33.69719529610905 36.0 33.5 39.0 26.0 41.0 74-75 33.34800611980852 35.5 33.0 39.0 26.0 40.0 76-77 33.01218336368622 35.0 33.0 38.0 25.5 40.0 78-79 32.688060493413616 35.0 33.0 37.0 25.0 39.0 80-81 32.32797506703524 35.0 33.0 37.0 24.5 39.0 82-83 32.00070305108429 35.0 32.5 36.0 24.0 39.0 84-85 31.70229860896817 35.0 32.0 36.0 23.5 37.0 86-87 31.381425584809094 35.0 32.0 35.5 22.0 37.0 88-89 31.123563948133025 35.0 32.0 35.0 20.5 37.0 90-91 30.910983245994032 35.0 32.0 35.0 19.5 36.0 92-93 30.666741632546195 35.0 32.0 35.0 18.0 36.0 94-95 30.45684488637403 35.0 32.0 35.0 14.0 36.0 96-97 30.19698162599025 34.5 31.5 35.0 4.5 35.5 98-99 29.98662779244043 34.0 31.0 35.0 2.0 35.0 100 29.860879455097212 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4387922165820157 1101 2 0.40117418635124125 1101 3 0.430189444645098 1101 4 0.4614322004428075 1101 5 0.4498400624627834 1101 6 0.5317652060531586 1101 7 0.5245777332140662 1101 8 0.5558892152777517 1101 9 0.6908320829872778 1101 10-11 0.6751091577260055 1101 12-13 0.6883079161173669 1101 14-15 0.7729934641501472 1101 16-17 0.7566458942185719 1101 18-19 0.6479350120489045 1101 20-21 0.6909079244773011 1101 22-23 0.7059218142002024 1101 24-25 0.6954950484237656 1101 26-27 0.6423344573988885 1101 28-29 0.631412725016709 1101 30-31 0.6757537538437717 1101 32-33 0.7042402104570797 1101 34-35 0.6055804132614142 1101 36-37 0.647922771259779 1101 38-39 0.6373435078742702 1101 40-41 0.7236220788294503 1101 42-43 0.7228123534485533 1101 44-45 0.6596149458104676 1101 46-47 0.6360735737560859 1101 48-49 0.6273296961782293 1101 50-51 0.709388999489434 1101 52-53 0.7239604653725351 1101 54-55 0.7872470021348477 1101 56-57 0.6957844437566081 1101 58-59 0.8511108206635711 1101 60-61 0.8414047097124637 1101 62-63 0.8343669472815023 1101 64-65 0.746249365519752 1101 66-67 0.6904976447432176 1101 68-69 0.7203584114069201 1101 70-71 0.7530435506689841 1101 72-73 0.801062100093425 1101 74-75 0.7399969441937415 1101 76-77 0.753052210527251 1101 78-79 0.7090036533430322 1101 80-81 0.7639034978141694 1101 82-83 0.8192055762615382 1101 84-85 0.7781394375819701 1101 86-87 0.7487077701034721 1101 88-89 0.7522866748980803 1101 90-91 0.7550029654021948 1101 92-93 0.7500749085653098 1101 94-95 0.7659871492362278 1101 96-97 0.7653999869021995 1101 98-99 0.754787015747354 1101 100 0.8158072350222625 1102 1 0.10607233008424188 1102 2 0.13907088534346812 1102 3 0.23343421615886584 1102 4 0.20515810068340556 1102 5 0.13280390801001118 1102 6 0.21856413641312145 1102 7 0.3303926922685463 1102 8 0.3828064803746827 1102 9 0.42512177109474436 1102 10-11 0.3944638754187295 1102 12-13 0.3842753765238598 1102 14-15 0.4383190955366416 1102 16-17 0.43107522803131815 1102 18-19 0.4607314453682321 1102 20-21 0.36888736841852676 1102 22-23 0.35357579732639977 1102 24-25 0.42600736347407064 1102 26-27 0.3209251331883678 1102 28-29 0.39099394905426976 1102 30-31 0.31957246902836545 1102 32-33 0.35698671970686036 1102 34-35 0.3253178702941497 1102 36-37 0.36189166886164514 1102 38-39 0.38702829076505907 1102 40-41 0.3846173664527015 1102 42-43 0.34750200233504813 1102 44-45 0.30914756953860945 1102 46-47 0.27358465043663927 1102 48-49 0.2584678881542146 1102 50-51 0.2942970143808452 1102 52-53 0.2709159635918965 1102 54-55 0.21812257684557324 1102 56-57 0.2345079852973484 1102 58-59 0.3903782026281206 1102 60-61 0.22605910681454588 1102 62-63 0.2412161711080998 1102 64-65 0.18267050101921 1102 66-67 0.3260642590734051 1102 68-69 0.37292804432875926 1102 70-71 0.3576996859256667 1102 72-73 0.32193269579175876 1102 74-75 0.3236219058936456 1102 76-77 0.36475250244513546 1102 78-79 0.2930892103143208 1102 80-81 0.22560285315868356 1102 82-83 0.19120202732867142 1102 84-85 0.24679570176137489 1102 86-87 0.29651311708978056 1102 88-89 0.24104884093380718 1102 90-91 0.23387083551505938 1102 92-93 0.2094221828542686 1102 94-95 0.23765174986041515 1102 96-97 0.19364810705818059 1102 98-99 0.2633563219672901 1102 100 0.20028331692670065 1103 1 0.07652720873520735 1103 2 0.1465703677905701 1103 3 0.1492357606011545 1103 4 0.157707109116501 1103 5 0.12252096768123977 1103 6 0.19381211876235227 1103 7 0.27523630469915616 1103 8 0.24121815483754006 1103 9 0.3216475412013722 1103 10-11 0.28202419953169766 1103 12-13 0.22523242849423042 1103 14-15 0.3366776976685557 1103 16-17 0.272875065555958 1103 18-19 0.3453220328330602 1103 20-21 0.3265312018059632 1103 22-23 0.2775247722494498 1103 24-25 0.3191865600225299 1103 26-27 0.25671282855955724 1103 28-29 0.3117374691711632 1103 30-31 0.28726808086449296 1103 32-33 0.29761703368627224 1103 34-35 0.3278006893504042 1103 36-37 0.30617918882871464 1103 38-39 0.33834360513514383 1103 40-41 0.3296533242961033 1103 42-43 0.3549612334649126 1103 44-45 0.2000762920327901 1103 46-47 0.24593652659609688 1103 48-49 0.24803270269305244 1103 50-51 0.18721846656580254 1103 52-53 0.23715950727054036 1103 54-55 0.2123332593387488 1103 56-57 0.18775712499152775 1103 58-59 0.2664106149321057 1103 60-61 0.2758834409032005 1103 62-63 0.24041562552276474 1103 64-65 0.29280414646849096 1103 66-67 0.2872699438583197 1103 68-69 0.3420156871793978 1103 70-71 0.2551707200809332 1103 72-73 0.2525744654118398 1103 74-75 0.2274531768021859 1103 76-77 0.3366264980501583 1103 78-79 0.22746302467514568 1103 80-81 0.2686954502189849 1103 82-83 0.20094604304777874 1103 84-85 0.2980237218738466 1103 86-87 0.27501745365946206 1103 88-89 0.29243645663809303 1103 90-91 0.27370342829799554 1103 92-93 0.22889923894894793 1103 94-95 0.23024893098942556 1103 96-97 0.258766053738551 1103 98-99 0.2799761386850932 1103 100 0.25093382251748153 1104 1 -0.032620851189193445 1104 2 -0.07663598084381817 1104 3 -0.015719708376149555 1104 4 0.045038096889278734 1104 5 0.024828598876553087 1104 6 0.07024349017640219 1104 7 0.12070662655072795 1104 8 0.11604376155079876 1104 9 0.17579020952454982 1104 10-11 0.2303262572335214 1104 12-13 0.18982273292706964 1104 14-15 0.24194682826950498 1104 16-17 0.29408676611643614 1104 18-19 0.27022283062903796 1104 20-21 0.220205778326644 1104 22-23 0.20349593609547156 1104 24-25 0.2518094178050063 1104 26-27 0.16697740802041494 1104 28-29 0.186039464772783 1104 30-31 0.15208701324311846 1104 32-33 0.17633697258656866 1104 34-35 0.21338955311596663 1104 36-37 0.16927311548607804 1104 38-39 0.2672041655435038 1104 40-41 0.21831581283919377 1104 42-43 0.24485367504567535 1104 44-45 0.23904178741418036 1104 46-47 0.07818088218571262 1104 48-49 0.10366024156606102 1104 50-51 0.11835440535416808 1104 52-53 0.1070016083926717 1104 54-55 0.1406970660162088 1104 56-57 0.14863237804027563 1104 58-59 0.22034513975244607 1104 60-61 0.09657470244588495 1104 62-63 0.10166519762551474 1104 64-65 0.0815922417823387 1104 66-67 0.14672241937569197 1104 68-69 0.0979557204221777 1104 70-71 0.09605888767251969 1104 72-73 0.08575716421779589 1104 74-75 0.07620388453369742 1104 76-77 0.15330459367834948 1104 78-79 0.10903475740889235 1104 80-81 0.0820477827333832 1104 82-83 0.07939954043938258 1104 84-85 0.10161219856729176 1104 86-87 0.16948414322398264 1104 88-89 0.15153489868959014 1104 90-91 0.18856550738222566 1104 92-93 0.14431341569763134 1104 94-95 0.17759706052738977 1104 96-97 0.1666579237674206 1104 98-99 0.12606459956598925 1104 100 0.14043321435713096 1105 1 -0.08600238065509913 1105 2 -0.06369563324126304 1105 3 0.0038702936957513145 1105 4 0.014809321492450067 1105 5 -0.001558726409982114 1105 6 0.021661045957280578 1105 7 0.08337338703353225 1105 8 0.14526583169104867 1105 9 0.18686635094917392 1105 10-11 0.14864433245634245 1105 12-13 0.09732868505700054 1105 14-15 0.13209722211394137 1105 16-17 0.11885868517296672 1105 18-19 0.10431531073054146 1105 20-21 0.07724134717332731 1105 22-23 0.17435681073082776 1105 24-25 0.15953340078608846 1105 26-27 0.11610992576449064 1105 28-29 0.19602569259382108 1105 30-31 0.16966125557846823 1105 32-33 0.1495090475856955 1105 34-35 0.20116701358942635 1105 36-37 0.12110627753619241 1105 38-39 0.17140311015775467 1105 40-41 0.25180325904770484 1105 42-43 0.12123122527092534 1105 44-45 0.021003957354025715 1105 46-47 0.007183340046374553 1105 48-49 -0.013430530592728473 1105 50-51 0.07893419598221385 1105 52-53 0.043651533309201795 1105 54-55 0.03203998295866484 1105 56-57 0.015607190239926183 1105 58-59 0.05624115876992164 1105 60-61 0.08423295988593082 1105 62-63 0.02597638240419542 1105 64-65 7.636924610210372E-4 1105 66-67 0.059042044856767006 1105 68-69 0.07839868769531222 1105 70-71 0.14367998383620773 1105 72-73 0.11966366604308121 1105 74-75 0.1539891696400275 1105 76-77 0.23985951829234153 1105 78-79 0.10446429170801252 1105 80-81 0.13279877788733785 1105 82-83 0.07016852491479142 1105 84-85 3.187963784085923E-4 1105 86-87 0.04651831858010169 1105 88-89 0.048426601095012956 1105 90-91 0.09645292755446988 1105 92-93 0.07684941496788156 1105 94-95 0.05229435449706088 1105 96-97 0.05274859599827053 1105 98-99 -0.027787851785607387 1105 100 0.007408588078465783 1106 1 0.032404831613973784 1106 2 -0.0026072595414419197 1106 3 0.031118343986349828 1106 4 0.0925964393592551 1106 5 0.049527441169260555 1106 6 0.15656916752391936 1106 7 0.18623550405011002 1106 8 0.19109673366769897 1106 9 0.27513650182455507 1106 10-11 0.23011551506227335 1106 12-13 0.24218552662622272 1106 14-15 0.2779467731930225 1106 16-17 0.29545871551272285 1106 18-19 0.32491537552694894 1106 20-21 0.22505375264128702 1106 22-23 0.3021592550945229 1106 24-25 0.25494644875870165 1106 26-27 0.20427915669206698 1106 28-29 0.2551785087313476 1106 30-31 0.22119700412904564 1106 32-33 0.2877692865805983 1106 34-35 0.3260443344909234 1106 36-37 0.27288532583456515 1106 38-39 0.2502761271104248 1106 40-41 0.29284995586319695 1106 42-43 0.22537858651801912 1106 44-45 0.20184553417982443 1106 46-47 0.2364034070623191 1106 48-49 0.2205954336117344 1106 50-51 0.2959180241786683 1106 52-53 0.31874928727260965 1106 54-55 0.4049787369346092 1106 56-57 0.2641998486465411 1106 58-59 0.2891925677336644 1106 60-61 0.34486055420317996 1106 62-63 0.2531354718894576 1106 64-65 0.3485643289693243 1106 66-67 0.3446577135932216 1106 68-69 0.30435334945630643 1106 70-71 0.2820082859019948 1106 72-73 0.2747761043567323 1106 74-75 0.2834422665321341 1106 76-77 0.32170981354556005 1106 78-79 0.20359687910708146 1106 80-81 0.25686914194645283 1106 82-83 0.21232866376166726 1106 84-85 0.21523290982223386 1106 86-87 0.24212147105032855 1106 88-89 0.2729345757957802 1106 90-91 0.28768206561913345 1106 92-93 0.262708394639354 1106 94-95 0.27943477250544646 1106 96-97 0.32733829337043474 1106 98-99 0.30408616038251424 1106 100 0.28875331030416973 1107 1 0.04282965320760823 1107 2 0.04738038314300752 1107 3 0.04126389917082207 1107 4 0.028044443024427324 1107 5 0.011645158413031709 1107 6 0.08970928990715521 1107 7 0.05858853648442164 1107 8 0.12080287807491175 1107 9 0.1173047590417795 1107 10-11 0.12952508411391506 1107 12-13 0.11869725219209926 1107 14-15 0.1967739861497222 1107 16-17 0.25717089475540433 1107 18-19 0.23476316042727063 1107 20-21 0.17331999308846946 1107 22-23 0.20816335667438324 1107 24-25 0.18184963584496927 1107 26-27 0.15413500469453822 1107 28-29 0.20032097170351904 1107 30-31 0.16471143946914424 1107 32-33 0.20495590459039903 1107 34-35 0.18726489125800327 1107 36-37 0.20571109125422282 1107 38-39 0.25832644433230456 1107 40-41 0.25506737380467115 1107 42-43 0.15113184797559853 1107 44-45 0.12826270892688285 1107 46-47 0.13201028073721233 1107 48-49 0.09036673698938813 1107 50-51 0.14630067008133096 1107 52-53 0.09222759381312073 1107 54-55 0.1276178047188239 1107 56-57 0.10287515399475211 1107 58-59 0.1662451339499782 1107 60-61 0.17709898970022664 1107 62-63 0.11808152481359002 1107 64-65 0.15444746225529826 1107 66-67 0.11456831722321681 1107 68-69 0.11248917664687497 1107 70-71 0.10562394394498398 1107 72-73 0.16082064418268516 1107 74-75 0.16799742177850874 1107 76-77 0.251364774706893 1107 78-79 0.0994838896545076 1107 80-81 0.16888769926789848 1107 82-83 0.20160453333987505 1107 84-85 0.21880124381867816 1107 86-87 0.214082790617951 1107 88-89 0.222492268334058 1107 90-91 0.21798482561690946 1107 92-93 0.2065173947822636 1107 94-95 0.16721935771779428 1107 96-97 0.09936091314439821 1107 98-99 0.09772433317806772 1107 100 0.03144655921009942 1108 1 -0.1440042067258247 1108 2 -0.08413573917801997 1108 3 -0.03154262368555649 1108 4 -0.10198194471238509 1108 5 -0.09635473300285469 1108 6 -0.02337519371054242 1108 7 -0.07476957651901017 1108 8 -0.008777013175702564 1108 9 -0.011243304862027514 1108 10-11 -0.010067099063363116 1108 12-13 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2214 92-93 -0.21770416431274242 2214 94-95 -0.18851000242780103 2214 96-97 -0.20613478830943777 2214 98-99 -0.11864291844315389 2214 100 -0.18119399744611542 2215 1 -0.16445892069162937 2215 2 -0.1377683054281711 2215 3 -0.25791651789773695 2215 4 -0.21967969885418626 2215 5 -0.148535848981318 2215 6 -0.1798178536772781 2215 7 -0.25819844676091463 2215 8 -0.1708783808529475 2215 9 -0.3300866842781005 2215 10-11 -0.2952035108201656 2215 12-13 -0.2515658390073341 2215 14-15 -0.3830811027286103 2215 16-17 -0.3463904304051866 2215 18-19 -0.4078081729709382 2215 20-21 -0.3403065357213251 2215 22-23 -0.32444194548930483 2215 24-25 -0.33302186941465095 2215 26-27 -0.36827196323234546 2215 28-29 -0.35763875027794967 2215 30-31 -0.3955295218579309 2215 32-33 -0.37355251918896926 2215 34-35 -0.3971876617636454 2215 36-37 -0.4669565447722803 2215 38-39 -0.3039707142434125 2215 40-41 -0.4531336498650589 2215 42-43 -0.2863893409070144 2215 44-45 -0.24134002561839196 2215 46-47 -0.27612067009982155 2215 48-49 -0.2734616291991898 2215 50-51 -0.1876883831595748 2215 52-53 -0.1780957710946609 2215 54-55 -0.2701101854595791 2215 56-57 -0.22014528548368872 2215 58-59 -0.21714861399284047 2215 60-61 -0.13360868914983826 2215 62-63 -0.11413403881403639 2215 64-65 -0.12126732970377674 2215 66-67 -0.09702308755170463 2215 68-69 -0.13666195397081538 2215 70-71 -0.16852098952099226 2215 72-73 -0.20093597566743426 2215 74-75 -0.2070718571112664 2215 76-77 -0.16206590752060634 2215 78-79 -0.16126370418879787 2215 80-81 -0.1618772861724267 2215 82-83 -0.12044148984749015 2215 84-85 -0.12292729893201582 2215 86-87 -0.00944224080849665 2215 88-89 0.04685063903966835 2215 90-91 -0.05181995316201338 2215 92-93 -0.019043807944228774 2215 94-95 -0.09194176928583175 2215 96-97 -0.04381588542311832 2215 98-99 -0.10005982958628934 2215 100 -0.07052525124937503 2216 1 -0.4583662508516042 2216 2 -0.41712401470788407 2216 3 -0.5151522849130004 2216 4 -0.6373054682925812 2216 5 -0.542184847713159 2216 6 -1.760853196391956 2216 7 -1.6734065516413779 2216 8 -2.5163589042330585 2216 9 -1.9446672368180415 2216 10-11 -1.107384560948141 2216 12-13 -0.9430970767575744 2216 14-15 -1.0917888021551363 2216 16-17 -1.3929359491694697 2216 18-19 -1.092009980880242 2216 20-21 -0.9791616177681419 2216 22-23 -1.072985209044404 2216 24-25 -1.2617851869907213 2216 26-27 -1.2933932685704121 2216 28-29 -1.6770632110379111 2216 30-31 -1.282447118193737 2216 32-33 -1.3862534721540314 2216 34-35 -2.3870002037750098 2216 36-37 -1.3906616134256495 2216 38-39 -1.226398272151222 2216 40-41 -1.653804827862757 2216 42-43 -1.4391929967005765 2216 44-45 -1.2593143896124417 2216 46-47 -1.064764628607989 2216 48-49 -1.2536304367630393 2216 50-51 -1.5870552635280717 2216 52-53 -1.7275499712278162 2216 54-55 -1.2740116243304982 2216 56-57 -1.163939604200145 2216 58-59 -2.1194684402568598 2216 60-61 -2.8160506398738363 2216 62-63 -1.8894495832836853 2216 64-65 -1.8702062359866503 2216 66-67 -1.4936645051847819 2216 68-69 -1.913294710359395 2216 70-71 -1.7739727820289701 2216 72-73 -2.1390931840999627 2216 74-75 -2.6036048951043753 2216 76-77 -3.271851060285705 2216 78-79 -2.2332423878530427 2216 80-81 -1.9538207772563112 2216 82-83 -2.3197482252200032 2216 84-85 -2.297977758210706 2216 86-87 -1.9952506685005673 2216 88-89 -1.9837028363873586 2216 90-91 -2.1443068027187486 2216 92-93 -2.1495944997257723 2216 94-95 -2.047025823971236 2216 96-97 -2.2628805714119338 2216 98-99 -2.0022687663660506 2216 100 -1.654406757393243 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 64641.0 3 11390.0 4 7299.0 5 6112.0 6 5422.0 7 5670.0 8 6424.0 9 7297.0 10 8233.0 11 9290.0 12 10372.0 13 11379.0 14 13126.0 15 13859.0 16 13961.0 17 14469.0 18 13843.0 19 12973.0 20 13291.0 21 13893.0 22 15576.0 23 18415.0 24 22528.0 25 28225.0 26 34414.0 27 40369.0 28 49224.0 29 62258.0 30 75858.0 31 90046.0 32 114989.0 33 153142.0 34 211582.0 35 306247.0 36 496971.0 37 853699.0 38 1103478.0 39 379027.0 40 751.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.81766951543337 20.628665771897854 14.523570335936528 23.030094376732244 2 15.245430404900478 20.50674911324463 40.68711759868707 23.56070288316782 3 18.047433951297005 25.53383747682781 30.657459158471728 25.761269413403454 4 12.541357917619731 17.157349638046572 39.36289527539562 30.938397168938074 5 12.928613363007807 37.923582057751744 35.80997066905359 13.337833910186859 6 30.371623874933768 38.383818499812875 17.81974762202962 13.424810003223739 7 26.406641487201803 32.81716897066721 22.92795691777968 17.8482326243513 8 24.295653555364062 36.82184285102572 20.843055051537938 18.03944854207228 9 24.411490959045853 17.35598263392027 21.958591251236374 36.2739351557975 10-11 23.41934240030662 27.35023485703335 29.362982501949663 19.867440240710366 12-13 23.809200137609636 24.924702030089545 29.29713133001151 21.968966502289305 14-15 21.364693965316604 27.220293514098483 26.412551941379625 25.002460579205295 16-17 20.951786131743606 30.539722481105386 27.029614111434817 21.478877275716187 18-19 20.84802585026121 29.52337017708832 29.032367132809238 20.596236839841232 20-21 21.95159145573006 28.694198542792442 28.564026213247335 20.79018378823016 22-23 21.59417574612426 28.571742864480772 28.605820502209202 21.228260887185765 24-25 21.524268023644446 28.66360437341884 28.494947054037382 21.317180548899334 26-27 21.219406065049533 28.642759268466595 28.586567094705984 21.55126757177789 28-29 21.266790869796292 28.687808216164395 28.691571362571626 21.353829551467683 30-31 21.58325936324666 29.01438728799841 28.277770277201984 21.124583071552948 32-33 21.223721523557344 28.801375759532945 28.667732003395397 21.30717071351431 34-35 21.432738993909865 28.484558942979056 28.69083647246088 21.3918655906502 36-37 21.69172662453599 28.756770588494113 28.20555958233661 21.345943204633294 38-39 21.49909188822178 28.9333119669055 28.32243514289532 21.245161001977397 40-41 21.38927057048298 28.694044535921947 28.523454380593783 21.393230513001292 42-43 21.30673893971255 28.68020601384333 28.464586435233596 21.548468611210527 44-45 21.426435998787486 28.75459584683884 28.396283630992837 21.422684523380838 46-47 21.398790670823043 28.85657918213797 28.261200559612604 21.483429587426386 48-49 21.356060784124608 28.71013955274161 28.325257104509 21.608542558624784 50-51 21.407045055555542 28.62931191992388 28.290161547704866 21.673481476815713 52-53 21.357911419959503 28.65419992848978 28.34311426999368 21.644774381557035 54-55 21.49343989381004 28.93818839316129 28.11534124895011 21.453030464078566 56-57 21.39881973766905 28.68649038686428 28.17340232245467 21.741287553011997 58-59 21.26449764220278 28.318240505621212 28.500232091954956 21.917029760221048 60-61 21.47927273754599 28.699029967058088 28.178878617882237 21.642818677513688 62-63 21.305089958053383 28.743736606561864 28.162460812542577 21.788712622842173 64-65 21.38636909914178 28.49769895995862 28.28218341537605 21.833748525523553 66-67 21.26296464696111 28.51537475513361 28.20489129547533 22.016769302429946 68-69 21.623790852246184 28.590924958592712 28.059221247770434 21.726062941390673 70-71 21.551329768295712 28.44911600613895 28.15929315226786 21.840261073297473 72-73 21.401681083635168 28.551097270800767 28.254510370526376 21.79271127503769 74-75 21.442666272389264 28.591298472124883 28.174123183360877 21.791912072124976 76-77 21.514077535571584 28.585345746867187 28.032525897872013 21.868050819689213 78-79 21.578030909403946 28.62516381956303 28.1006633368026 21.69614193423042 80-81 21.53927986347688 28.45816710141593 28.177009044691314 21.82554399041587 82-83 21.398869203242498 28.536426010247407 28.26782328281648 21.796881503693623 84-85 21.453918918433878 28.40706561879509 28.153951303079644 21.985064159691387 86-87 21.61020030187232 28.497791533958782 28.088054739020308 21.80395342514859 88-89 21.48800941281848 28.365447907212136 28.15406010582937 21.992482574140013 90-91 21.453402421673534 28.29430132347274 28.1873040912055 22.06499216364822 92-93 21.54510181603077 28.461538372474166 27.993124307686536 22.000235503808526 94-95 21.441852780232097 28.28418284885663 28.152037576358673 22.1219267945526 96-97 21.490850265860722 28.242675548059225 28.10538728808635 22.1610868979937 98-99 21.53567932166335 28.336118607056022 28.013842490166663 22.11435958111397 100 21.588969528974296 28.198552552779184 28.05264109462067 22.15983682362585 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2075.0 1 1587.0 2 1356.5 3 1628.0 4 1556.0 5 1295.5 6 1020.0 7 886.5 8 880.0 9 872.0 10 758.5 11 689.0 12 745.0 13 816.5 14 904.5 15 1010.0 16 1150.5 17 1462.0 18 1941.5 19 2588.0 20 3615.0 21 5194.5 22 7585.5 23 11047.5 24 15874.0 25 22594.0 26 31630.0 27 42480.0 28 55161.5 29 70294.5 30 85729.5 31 98532.5 32 107828.0 33 117514.0 34 127060.5 35 134017.0 36 139697.0 37 146658.0 38 153802.0 39 156750.5 40 158150.0 41 158901.0 42 157316.0 43 155208.0 44 152279.5 45 149347.5 46 146079.0 47 140609.0 48 135077.5 49 130134.0 50 126465.5 51 121360.0 52 112448.5 53 104360.0 54 98434.5 55 93855.0 56 87584.5 57 79418.0 58 72445.0 59 65550.5 60 57665.5 61 49154.0 62 41790.5 63 35121.0 64 28819.0 65 23430.0 66 18903.0 67 14929.0 68 11496.0 69 8996.0 70 6876.5 71 5148.0 72 4004.5 73 3017.5 74 2221.0 75 1601.0 76 1180.5 77 878.0 78 621.0 79 454.5 80 324.5 81 192.5 82 123.5 83 101.0 84 71.5 85 61.5 86 54.5 87 41.0 88 30.5 89 18.5 90 13.0 91 10.5 92 14.5 93 12.5 94 8.0 95 8.0 96 7.5 97 8.5 98 11.0 99 11.5 100 22.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013056332286434633 2 0.034076101286581166 3 0.03537247470509241 4 0.03810411869409824 5 0.041159856037731875 6 0.041090407461740204 7 0.03875230540335386 8 0.03847451109938716 9 0.03865970730203163 10-11 0.03913427257130806 12-13 0.040083403109860936 14-15 0.0382893148967427 16-17 0.03505995611312988 18-19 0.03804624488077184 20-21 0.03752538056083429 22-23 0.03847451109938716 24-25 0.03517570373978267 26-27 0.035326175654431295 28-29 0.03583546521170357 30-31 0.03602066141434803 32-33 0.03580074092370773 34-35 0.03545349804374936 36-37 0.03317326979868941 38-39 0.03338161552666443 40-41 0.03468956370784095 42-43 0.03448121797986593 44-45 0.03338161552666443 46-47 0.03237461117478517 48-49 0.03267555500408242 50-51 0.033613110779970014 52-53 0.03423814796389507 54-55 0.03342791457732555 56-57 0.0319694944815004 58-59 0.03267555500408242 60-61 0.03205051782015736 62-63 0.03233988688678933 64-65 0.029191551441833462 66-67 0.030476350097679423 68-69 0.031494929212223965 70-71 0.03276815310540465 72-73 0.029596668135118222 74-75 0.029967060540407146 76-77 0.030974064892286414 78-79 0.031217134908257272 80-81 0.03133288253491006 82-83 0.029712415761771013 84-85 0.030985639654951696 86-87 0.031506503974889244 88-89 0.03186532161751289 90-91 0.03257138214009491 92-93 0.031055088230943367 94-95 0.032166265446810144 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4319743.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.54445715909215 #Duplication Level Percentage of deduplicated Percentage of total 1 79.00012105019756 51.780200497377685 2 12.87478437002788 16.877415051476834 3 3.6892968352168154 7.254388750891285 4 1.5709778314349438 4.118755566814847 5 0.8260002546837607 2.706986915325948 6 0.4785224070078394 1.8818694843474593 7 0.31847580008018456 1.4612026404194276 8 0.22358136184016147 1.1723615194163157 9 0.1645911543543051 0.9709234078807147 >10 0.8087699301510454 9.106835086098213 >50 0.035392185351150476 1.5821319627178216 >100 0.009380886028123488 0.9949064285888275 >500 7.062241953362742E-5 0.030643371961057268 >1k 3.531120665646376E-5 0.06137931668359523 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.314952533055786E-5 0.0 5 0.0 4.629905066111572E-5 0.0 2.314952533055786E-5 0.0 6 0.0 4.629905066111572E-5 0.0 2.314952533055786E-5 0.0 7 0.0 6.944857599167358E-5 0.0 2.314952533055786E-5 0.0 8 0.0 6.944857599167358E-5 0.0 2.314952533055786E-5 0.0 9 0.0 6.944857599167358E-5 0.0 2.314952533055786E-5 0.0 10-11 2.314952533055786E-5 8.102333865695251E-5 0.0 2.314952533055786E-5 0.0 12-13 2.314952533055786E-5 1.0417286398751037E-4 0.0 6.944857599167358E-5 0.0 14-15 2.314952533055786E-5 1.2732238931806823E-4 0.0 9.259810132223144E-5 0.0 16-17 4.629905066111572E-5 1.504719146486261E-4 0.0 1.157476266527893E-4 0.0 18-19 4.629905066111572E-5 1.851962026444629E-4 0.0 1.504719146486261E-4 0.0 20-21 4.629905066111572E-5 1.851962026444629E-4 0.0 2.546447786361364E-4 0.0 22-23 5.787381332639465E-5 1.851962026444629E-4 0.0 5.208643199375518E-4 0.0 24-25 6.944857599167358E-5 2.314952533055786E-4 0.0 0.0010070043518792668 0.0 26-27 6.944857599167358E-5 2.777943039666943E-4 0.0 0.0018172377384487919 0.0 28-29 6.944857599167358E-5 2.777943039666943E-4 0.0 0.004710928404768524 0.0 30-31 8.102333865695251E-5 2.777943039666943E-4 0.0 0.013091056574430469 0.0 32-33 9.259810132223144E-5 2.777943039666943E-4 0.0 0.03275657834273937 0.0 34-35 9.259810132223144E-5 3.0094382929725215E-4 0.0 0.06660118437601496 0.0 36-37 9.259810132223144E-5 3.472428799583679E-4 0.0 0.11671990671667273 0.0 38-39 9.259810132223144E-5 3.935419306194836E-4 0.0 0.18033480232504573 0.0 40-41 9.259810132223144E-5 4.3984098128059936E-4 0.0 0.24873007491417892 0.0 42-43 1.157476266527893E-4 4.3984098128059936E-4 0.0 0.31790085660188583 0.0 44-45 1.157476266527893E-4 4.3984098128059936E-4 0.0 0.40326473125831797 0.0 46-47 1.6204667731390502E-4 4.3984098128059936E-4 0.0 0.504729100782153 0.0 48-49 1.6204667731390502E-4 4.3984098128059936E-4 0.0 0.609469128140262 0.0 50-51 2.314952533055786E-4 4.3984098128059936E-4 0.0 0.7127275858772153 0.0 52-53 2.777943039666943E-4 4.3984098128059936E-4 0.0 0.8126525119665684 0.0 54-55 2.777943039666943E-4 4.3984098128059936E-4 0.0 0.9252981022250629 0.0 56-57 2.777943039666943E-4 4.3984098128059936E-4 0.0 1.059044484822361 0.0 58-59 2.893690666319732E-4 4.3984098128059936E-4 0.0 1.1992727345122152 0.0 60-61 3.0094382929725215E-4 4.3984098128059936E-4 0.0 1.3441540387935116 0.0 62-63 3.0094382929725215E-4 4.3984098128059936E-4 0.0 1.4880283387229287 0.0 64-65 3.0094382929725215E-4 4.3984098128059936E-4 0.0 1.637643720934324 0.0 66-67 3.0094382929725215E-4 4.3984098128059936E-4 0.0 1.812758305297329 0.0 68-69 3.0094382929725215E-4 4.3984098128059936E-4 0.0 2.004714169338315 0.0 70-71 3.2409335462781E-4 4.3984098128059936E-4 0.0 2.2083258193832362 0.0 72-73 3.472428799583679E-4 4.977147946069939E-4 0.0 2.4121921142067944 0.0 74-75 3.472428799583679E-4 5.092895572722729E-4 0.0 2.6191025716113203 0.0 76-77 3.703924052889258E-4 5.092895572722729E-4 0.0 2.842588089152526 0.0 78-79 3.703924052889258E-4 5.092895572722729E-4 0.0 3.0922209955545967 0.0 80-81 3.935419306194836E-4 5.092895572722729E-4 0.0 3.360767064151733 0.0 82-83 4.1669145595004146E-4 5.208643199375518E-4 0.0 3.636767279905309 0.0 84-85 4.1669145595004146E-4 5.671633705986675E-4 0.0 3.907315782443539 0.0 86-87 4.1669145595004146E-4 5.903128959292254E-4 0.0 4.1955042232836535 0.0 88 4.1669145595004146E-4 6.250371839250622E-4 0.0 4.42982371867956 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3805 0.0 48.311142 1 GTATCAA 6920 0.0 36.007668 1 TCAACGC 7570 0.0 31.36177 4 ATCAACG 7690 0.0 31.055779 3 CAACGCA 8005 0.0 29.77534 5 AACGCAG 8525 0.0 28.174822 6 TATCAAC 8810 0.0 27.214437 2 GTACATG 11290 0.0 23.944132 1 ACGCAGA 9950 0.0 23.761263 7 CGCAGAG 10125 0.0 23.118462 8 TACATGG 11525 0.0 22.394247 2 ACATGGG 11765 0.0 21.338034 3 GCAGAGT 11690 0.0 19.098917 9 CATGGGG 8790 0.0 18.237722 4 AGAGTAC 9870 0.0 18.096561 10-11 GTATAGA 2190 0.0 17.38866 1 CAGAGTA 11085 0.0 16.770283 10-11 GAGTACA 8695 0.0 15.734344 1 GTACTAT 1790 0.0 15.496163 1 GTGGTAT 2705 0.0 15.468492 1 >>END_MODULE