FastQCFastQC Report
Wed 25 May 2016
SRR2049359_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049359_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4319743
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124910.28916072090399825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120510.27897492975855276No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68960.159639126679527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74300.058.4696431
GTATCAA139450.041.1665421
ATCAACG173100.032.823163
TCAACGC173700.032.7097854
CAACGCA179850.031.5912655
TATCAAC181500.031.4076422
AACGCAG187700.030.4008186
TGGTATC32950.030.0938822
GTGGTAT34600.029.758791
ACGCAGA214050.026.438647
CGCAGAG214800.026.2806248
GCAGAGT236250.023.4763589
GTACATG108400.023.0309851
TACATGG109450.021.7263072
GAGTACT174800.021.39777812-13
CAGAGTA226150.021.21740510-11
AGAGTAC215250.020.70736310-11
ACATGGG111050.020.5669023
GTACTTT189900.019.51317214-15
AGTACTT181650.019.4260812-13
Produced by FastQC (version 0.11.5)