##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049359_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4319743 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.772032271364292 31.0 31.0 34.0 30.0 34.0 2 32.02666732719979 33.0 31.0 34.0 30.0 34.0 3 31.977053958997097 33.0 31.0 34.0 30.0 34.0 4 35.69403920557311 37.0 35.0 37.0 33.0 37.0 5 35.7949891926441 37.0 35.0 37.0 35.0 37.0 6 35.85392811563095 37.0 35.0 37.0 35.0 37.0 7 35.80485181641593 37.0 35.0 37.0 35.0 37.0 8 35.79483316484337 37.0 35.0 37.0 35.0 37.0 9 37.529889625378175 39.0 37.0 39.0 35.0 39.0 10-11 37.491540468958455 39.0 37.0 39.0 35.0 39.0 12-13 37.442620081796534 39.0 37.0 39.0 34.5 39.0 14-15 38.72697461399903 40.0 38.0 41.0 34.0 41.0 16-17 38.75207749627698 40.0 38.0 41.0 35.0 41.0 18-19 38.83049257791494 40.0 38.0 41.0 35.0 41.0 20-21 38.8631893147347 40.0 38.0 41.0 35.0 41.0 22-23 38.82937943298941 40.0 38.0 41.0 35.0 41.0 24-25 38.80882427496265 40.0 38.0 41.0 35.0 41.0 26-27 38.69183988491908 40.0 38.0 41.0 34.5 41.0 28-29 38.60763024559563 40.0 38.0 41.0 34.0 41.0 30-31 38.497491401687554 40.0 38.0 41.0 34.0 41.0 32-33 38.35002140173617 40.0 38.0 41.0 34.0 41.0 34-35 38.23956679830258 40.0 38.0 41.0 34.0 41.0 36-37 38.12291634016191 40.0 38.0 41.0 34.0 41.0 38-39 38.02030178647202 40.0 38.0 41.0 33.0 41.0 40-41 37.90155340259825 40.0 37.5 41.0 33.0 41.0 42-43 37.754086643580415 40.0 37.0 41.0 33.0 41.0 44-45 37.56651286893688 40.0 37.0 41.0 33.0 41.0 46-47 37.32308496130442 40.0 37.0 41.0 32.0 41.0 48-49 37.10909954596836 40.0 37.0 41.0 31.5 41.0 50-51 36.961935814237094 40.0 37.0 41.0 31.0 41.0 52-53 36.824403905510124 40.0 36.0 41.0 31.0 41.0 54-55 36.690021721199614 40.0 36.0 41.0 31.0 41.0 56-57 36.5537859543959 39.0 35.5 41.0 31.0 41.0 58-59 36.37549189847637 39.0 35.0 41.0 30.5 41.0 60-61 36.202324304941286 39.0 35.0 41.0 30.0 41.0 62-63 36.003781822205625 39.0 35.0 41.0 30.0 41.0 64-65 35.780695749724 38.5 35.0 40.5 30.0 41.0 66-67 35.51885065847667 38.0 35.0 40.0 29.0 41.0 68-69 35.23284521787523 37.0 35.0 40.0 29.0 41.0 70-71 34.904588536864345 37.0 34.0 39.5 29.0 41.0 72-73 34.615166226324106 36.0 34.0 39.0 29.0 41.0 74-75 34.30625479339859 36.0 34.0 39.0 29.0 41.0 76-77 33.975530488735096 35.5 34.0 38.5 28.5 40.0 78-79 33.63656715225883 35.0 34.0 37.0 28.0 39.0 80-81 33.31686364211945 35.0 34.0 37.0 28.0 39.0 82-83 33.01463154173756 35.0 34.0 36.5 27.5 39.0 84-85 32.74073619657466 35.0 33.5 36.0 27.0 37.5 86-87 32.50358632446421 35.0 33.0 36.0 27.0 37.0 88-89 32.268500464032236 35.0 33.0 35.0 26.0 37.0 90-91 32.07072828175195 35.0 33.0 35.0 26.0 36.0 92-93 31.891092942334765 35.0 33.0 35.0 26.0 36.0 94-95 31.732791510976462 35.0 33.0 35.0 26.0 36.0 96-97 31.540745132291434 35.0 33.0 35.0 25.0 36.0 98-99 31.389927247986744 35.0 33.0 35.0 25.0 35.0 100 31.27577288741483 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2527917219645488 1101 2 0.2650142972333853 1101 3 0.4347455017451445 1101 4 0.22466111808497402 1101 5 0.24377075504554568 1101 6 0.21472008846159696 1101 7 0.1591015700267704 1101 8 0.23136511370231005 1101 9 0.29902226250903396 1101 10-11 0.33475143199203217 1101 12-13 0.2758066213698882 1101 14-15 0.3940196403704235 1101 16-17 0.2739648420332941 1101 18-19 0.39927526182986384 1101 20-21 0.33545752310593 1101 22-23 0.3530965396740271 1101 24-25 0.3585929396506202 1101 26-27 0.4574958132771698 1101 28-29 0.46304036568140106 1101 30-31 0.41963969632477927 1101 32-33 0.4776271138615513 1101 34-35 0.38130394890984576 1101 36-37 0.45911498020989683 1101 38-39 0.5366291287980189 1101 40-41 0.5017444920569005 1101 42-43 0.5890547090024612 1101 44-45 0.6045674804394778 1101 46-47 0.5869852640321085 1101 48-49 0.6228670802723144 1101 50-51 0.6241421479076763 1101 52-53 0.6689015441756254 1101 54-55 0.6640760753013808 1101 56-57 0.6665040576973595 1101 58-59 0.7048747399559403 1101 60-61 0.6526291023835071 1101 62-63 0.6389987034238729 1101 64-65 0.6142651036715421 1101 66-67 0.6087351677376631 1101 68-69 0.6089219676093549 1101 70-71 0.6426212726303504 1101 72-73 0.5798278572118463 1101 74-75 0.6398751604983772 1101 76-77 0.5708794663082699 1101 78-79 0.5868404246335359 1101 80-81 0.6523900133793319 1101 82-83 0.6045760910337847 1101 84-85 0.6346403086794226 1101 86-87 0.5471302347906075 1101 88-89 0.6021051476592092 1101 90-91 0.6412515226282665 1101 92-93 0.6278220826599821 1101 94-95 0.6664967793255201 1101 96-97 0.6245057301610437 1101 98-99 0.6758238885736993 1101 100 0.7525958646988293 1102 1 0.20569699348083503 1102 2 0.18541785675633804 1102 3 0.36264834549583114 1102 4 0.17949750970532818 1102 5 0.19400993181535142 1102 6 0.18080849345308536 1102 7 0.20821908769504205 1102 8 0.14693605929189602 1102 9 0.16081490370034857 1102 10-11 0.20905881244132019 1102 12-13 0.20671534240623402 1102 14-15 0.3040223695138238 1102 16-17 0.20979263999129216 1102 18-19 0.27358593859882774 1102 20-21 0.22110173175479986 1102 22-23 0.23421678502066356 1102 24-25 0.2140363720842302 1102 26-27 0.279479220336448 1102 28-29 0.3084896969575439 1102 30-31 0.22103206698766087 1102 32-33 0.25161914116302597 1102 34-35 0.2776801634205128 1102 36-37 0.33885152996987955 1102 38-39 0.40132553984887664 1102 40-41 0.3934693640550506 1102 42-43 0.3877346009919975 1102 44-45 0.416251196578429 1102 46-47 0.3909397122428473 1102 48-49 0.3029096802334763 1102 50-51 0.4223085988659747 1102 52-53 0.4072362874268549 1102 54-55 0.3454990483682465 1102 56-57 0.40241014586376167 1102 58-59 0.35620103248997737 1102 60-61 0.4247923786196992 1102 62-63 0.4084630786844414 1102 64-65 0.29331114247895584 1102 66-67 0.2970844409567235 1102 68-69 0.358050221861717 1102 70-71 0.3491617045549731 1102 72-73 0.3130484128101827 1102 74-75 0.3002271163984318 1102 76-77 0.2696854503737569 1102 78-79 0.4016246695230876 1102 80-81 0.34467956586679094 1102 82-83 0.4038888502396176 1102 84-85 0.3968343631233182 1102 86-87 0.3419121241196521 1102 88-89 0.4163031708719984 1102 90-91 0.401634877281289 1102 92-93 0.39392198814456236 1102 94-95 0.3505256500629059 1102 96-97 0.40507030495883 1102 98-99 0.37572160848322156 1102 100 0.4645612016475802 1103 1 0.12453966464987332 1103 2 0.14951660182146043 1103 3 0.30950276169213353 1103 4 0.1844400391816805 1103 5 0.17768199378636496 1103 6 0.12718200835820426 1103 7 0.12804938874569416 1103 8 0.1207238678400202 1103 9 0.2409262017391285 1103 10-11 0.1973026587977884 1103 12-13 0.24165940138228592 1103 14-15 0.3063059961539878 1103 16-17 0.28776784920751197 1103 18-19 0.26293509770626144 1103 20-21 0.30342428461567295 1103 22-23 0.30386090296150314 1103 24-25 0.25219346288113087 1103 26-27 0.2831614732663539 1103 28-29 0.3136381903348848 1103 30-31 0.29077254643093653 1103 32-33 0.27440629541957406 1103 34-35 0.32441252171372525 1103 36-37 0.2977488935916739 1103 38-39 0.31244302918970135 1103 40-41 0.32511648521236935 1103 42-43 0.3252391320971313 1103 44-45 0.3377487681074598 1103 46-47 0.3824788299782753 1103 48-49 0.3865410931569073 1103 50-51 0.42353871839048907 1103 52-53 0.4432666285123332 1103 54-55 0.3493483480406425 1103 56-57 0.39053087202471914 1103 58-59 0.4417655407261165 1103 60-61 0.40251844753760935 1103 62-63 0.3434305433357778 1103 64-65 0.3762685786870321 1103 66-67 0.3222852142250616 1103 68-69 0.3336959597229807 1103 70-71 0.37898125983807773 1103 72-73 0.32809137976976643 1103 74-75 0.3260767690942643 1103 76-77 0.3779301787853555 1103 78-79 0.3724436728168996 1103 80-81 0.4252739300580899 1103 82-83 0.4054367260978111 1103 84-85 0.3690577572795206 1103 86-87 0.33138563689012557 1103 88-89 0.3779345026383112 1103 90-91 0.3642521523511846 1103 92-93 0.39675292193887657 1103 94-95 0.372419471770165 1103 96-97 0.4030851774555444 1103 98-99 0.4318400503630393 1103 100 0.46753703011484404 1104 1 0.15910063344375303 1104 2 0.17779749561976388 1104 3 0.2707866134919108 1104 4 0.16079318158627132 1104 5 0.14046501930840094 1104 6 0.15010395625766648 1104 7 0.1922391276833295 1104 8 0.21036496410798833 1104 9 0.17506090724423018 1104 10-11 0.19646404247471594 1104 12-13 0.2166628825396657 1104 14-15 0.27414817595555263 1104 16-17 0.24162489252087482 1104 18-19 0.24279221787821115 1104 20-21 0.31876163392205115 1104 22-23 0.27029721507943805 1104 24-25 0.2786213411585905 1104 26-27 0.2938194810112478 1104 28-29 0.32744190174219057 1104 30-31 0.36337102940341026 1104 32-33 0.35568625301893064 1104 34-35 0.3073420807217673 1104 36-37 0.35293612715015854 1104 38-39 0.3023875635474127 1104 40-41 0.3119170528925821 1104 42-43 0.32026998957447717 1104 44-45 0.3181900902170085 1104 46-47 0.39300165237896323 1104 48-49 0.4040527283205009 1104 50-51 0.343415952129142 1104 52-53 0.35995000217886286 1104 54-55 0.2977923473590707 1104 56-57 0.3130139185726577 1104 58-59 0.36673793823873524 1104 60-61 0.3216700767863401 1104 62-63 0.3641923767791937 1104 64-65 0.2960423240616308 1104 66-67 0.358409673100347 1104 68-69 0.2887679820660267 1104 70-71 0.36156030726691313 1104 72-73 0.3774117370989529 1104 74-75 0.3727690322902717 1104 76-77 0.323631287235699 1104 78-79 0.351980301300884 1104 80-81 0.3668681592622818 1104 82-83 0.3401390064020404 1104 84-85 0.3663268935346622 1104 86-87 0.3697377489871272 1104 88-89 0.38837039193817446 1104 90-91 0.3215275460645728 1104 92-93 0.4080553990087523 1104 94-95 0.44940406928991194 1104 96-97 0.4353452282013812 1104 98-99 0.459161361812054 1104 100 0.4229825281757442 1105 1 0.251459560859395 1105 2 0.2601331233230866 1105 3 0.3141141543661057 1105 4 0.2351934305810346 1105 5 0.2185673235183927 1105 6 0.16268813867291954 1105 7 0.20191247332582662 1105 8 0.20622279048273384 1105 9 0.18997744449352183 1105 10-11 0.24701586932251018 1105 12-13 0.17814292995502257 1105 14-15 0.2351573587833471 1105 16-17 0.2314782070115271 1105 18-19 0.23789393810564263 1105 20-21 0.26668564238934067 1105 22-23 0.25137387677109757 1105 24-25 0.2693355829988846 1105 26-27 0.27133349384236993 1105 28-29 0.30755757062683386 1105 30-31 0.3365160367650688 1105 32-33 0.31654443235016316 1105 34-35 0.2979419669505887 1105 36-37 0.29336283550541964 1105 38-39 0.2952637326584906 1105 40-41 0.33075497114603536 1105 42-43 0.3482016370287013 1105 44-45 0.3382976892327676 1105 46-47 0.4076396748691735 1105 48-49 0.24875354497930857 1105 50-51 0.2862486892563183 1105 52-53 0.31014965178931675 1105 54-55 0.2830774078567302 1105 56-57 0.2952450688624424 1105 58-59 0.278324560179243 1105 60-61 0.27259200372709813 1105 62-63 0.2910186286746139 1105 64-65 0.2537442243427108 1105 66-67 0.3636800125619857 1105 68-69 0.3708167982407886 1105 70-71 0.32207744811763916 1105 72-73 0.3233269189036392 1105 74-75 0.30962753386619823 1105 76-77 0.27236282119378075 1105 78-79 0.32750820044883966 1105 80-81 0.35210221296300404 1105 82-83 0.3044777263709406 1105 84-85 0.28065584990190473 1105 86-87 0.2579734111610108 1105 88-89 0.28057649377666394 1105 90-91 0.31534790817043756 1105 92-93 0.3271514682652352 1105 94-95 0.3153290192801066 1105 96-97 0.3888401621297568 1105 98-99 0.3958197095539582 1105 100 0.43323951258881976 1106 1 0.1859741830086712 1106 2 0.20793572878280742 1106 3 0.2823557957198126 1106 4 0.17880378046370282 1106 5 0.19241347323387714 1106 6 0.14549503107688366 1106 7 0.1467088479492631 1106 8 0.17334687290662032 1106 9 0.22564522331941816 1106 10-11 0.1970696351211032 1106 12-13 0.19591721383843463 1106 14-15 0.24719448738988348 1106 16-17 0.2439710807498301 1106 18-19 0.18861732638465867 1106 20-21 0.25195946312167905 1106 22-23 0.20491585257806122 1106 24-25 0.15702907722538129 1106 26-27 0.18567909493638268 1106 28-29 0.23998991477090215 1106 30-31 0.23965888598097962 1106 32-33 0.18507338399092532 1106 34-35 0.20838467001661343 1106 36-37 0.22521716934650726 1106 38-39 0.2703901259153696 1106 40-41 0.2917731897640792 1106 42-43 0.22959518029998804 1106 44-45 0.25442028017594964 1106 46-47 0.3270165285921891 1106 48-49 0.3524628480958043 1106 50-51 0.3537553076180444 1106 52-53 0.2886545419486808 1106 54-55 0.36068617899834976 1106 56-57 0.3689348771223777 1106 58-59 0.417263684676044 1106 60-61 0.30851970651567484 1106 62-63 0.3780094981098685 1106 64-65 0.3050919492193387 1106 66-67 0.2797733153526991 1106 68-69 0.35518717232366015 1106 70-71 0.3868723728768515 1106 72-73 0.3986616518332724 1106 74-75 0.3585173696914552 1106 76-77 0.34153112268678854 1106 78-79 0.3735242286084315 1106 80-81 0.3968918193159183 1106 82-83 0.4277715180881003 1106 84-85 0.38741487978163036 1106 86-87 0.3930541963430372 1106 88-89 0.41309325671312536 1106 90-91 0.3814972142045576 1106 92-93 0.48085217959368975 1106 94-95 0.4786590777400619 1106 96-97 0.4692862146506407 1106 98-99 0.5195004561624366 1106 100 0.5394499657519418 1107 1 0.12582355411085544 1107 2 0.10865683586771269 1107 3 0.21743894466542812 1107 4 0.1978763726907644 1107 5 0.15146019572495817 1107 6 0.09420293375481492 1107 7 0.08587573224991019 1107 8 0.10726403617444902 1107 9 0.11374156498603583 1107 10-11 0.1129752466001861 1107 12-13 0.07834113746206128 1107 14-15 0.18736328590475182 1107 16-17 0.12949181924572883 1107 18-19 0.17671020360708667 1107 20-21 0.14321854637969977 1107 22-23 0.15113343573347748 1107 24-25 0.12259379675327153 1107 26-27 0.2642415255590933 1107 28-29 0.2657862815743286 1107 30-31 0.2311123067137757 1107 32-33 0.2330947260987628 1107 34-35 0.2373945590680009 1107 36-37 0.19669149730363245 1107 38-39 0.24729753801319987 1107 40-41 0.2937771161196565 1107 42-43 0.3259047594642439 1107 44-45 0.29072922216102626 1107 46-47 0.39444688613697565 1107 48-49 0.4086078222024554 1107 50-51 0.46429638612259083 1107 52-53 0.420661355848857 1107 54-55 0.41005855641463995 1107 56-57 0.3893939325168603 1107 58-59 0.4070253380968296 1107 60-61 0.31971838092183447 1107 62-63 0.3431003700240751 1107 64-65 0.30728603917785335 1107 66-67 0.3216465094268415 1107 68-69 0.3145052182456922 1107 70-71 0.27808305837175595 1107 72-73 0.35899267773506693 1107 74-75 0.31593811013935635 1107 76-77 0.285309047287555 1107 78-79 0.3361636145470044 1107 80-81 0.32435209207518056 1107 82-83 0.3369409835302335 1107 84-85 0.36555190842926066 1107 86-87 0.35744960067076903 1107 88-89 0.34505053907165717 1107 90-91 0.3511671151286322 1107 92-93 0.4198017309536013 1107 94-95 0.48641369855675265 1107 96-97 0.47369748360843644 1107 98-99 0.4945543470934233 1107 100 0.49831689403492163 1108 1 0.14737075606407046 1108 2 0.13407780588261176 1108 3 0.22767545892370933 1108 4 0.1520741953824185 1108 5 0.1302434345688539 1108 6 0.11608873315842771 1108 7 0.1016088032230229 1108 8 0.03074210421823409 1108 9 0.14885959689734563 1108 10-11 0.13319641436147833 1108 12-13 0.09845576562978664 1108 14-15 0.17540991387362936 1108 16-17 0.1346378860668267 1108 18-19 0.08118242382285246 1108 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-0.40302542908888483 2215 20-21 -0.4117534619609273 2215 22-23 -0.3996518902347006 2215 24-25 -0.42435795907773155 2215 26-27 -0.43204851557064217 2215 28-29 -0.47654449461325754 2215 30-31 -0.468750917256493 2215 32-33 -0.42773711463187425 2215 34-35 -0.4727817486239445 2215 36-37 -0.4598970008107841 2215 38-39 -0.40384713981215725 2215 40-41 -0.46668333919726024 2215 42-43 -0.4313048868153473 2215 44-45 -0.4480730598297953 2215 46-47 -0.4767090585828484 2215 48-49 -0.4118887581669597 2215 50-51 -0.43018088495538365 2215 52-53 -0.3759940429240345 2215 54-55 -0.33234691736063127 2215 56-57 -0.39956179880470444 2215 58-59 -0.41897267213930434 2215 60-61 -0.43315704650576237 2215 62-63 -0.36308768606449604 2215 64-65 -0.3037486821995472 2215 66-67 -0.2861772449576563 2215 68-69 -0.35656288011241344 2215 70-71 -0.3844575333966276 2215 72-73 -0.3730136726890976 2215 74-75 -0.35470775448327174 2215 76-77 -0.23692888924190214 2215 78-79 -0.2831598709361671 2215 80-81 -0.38661913785162483 2215 82-83 -0.24225059090964152 2215 84-85 -0.34752307537760174 2215 86-87 -0.2658726690254767 2215 88-89 -0.2806981381115108 2215 90-91 -0.26490467194125245 2215 92-93 -0.29489494497676105 2215 94-95 -0.34069782037487073 2215 96-97 -0.3716891224255754 2215 98-99 -0.2822938396078989 2215 100 -0.29036659830584455 2216 1 -0.6714522728106864 2216 2 -0.6727392644602475 2216 3 -0.8427293448097686 2216 4 -0.5193140882664551 2216 5 -0.7659633457424988 2216 6 -0.5952589386953235 2216 7 -0.7606160449517745 2216 8 -0.5443518684012503 2216 9 -0.8266641337640905 2216 10-11 -0.7509900338890034 2216 12-13 -0.678431264577533 2216 14-15 -1.0000692739779922 2216 16-17 -0.8521055187179556 2216 18-19 -0.8225424802865078 2216 20-21 -0.7553579908152415 2216 22-23 -0.8190064860562245 2216 24-25 -0.7332956058751066 2216 26-27 -0.9252333756422573 2216 28-29 -0.9658226679577027 2216 30-31 -1.0427224172900438 2216 32-33 -0.9800445513917779 2216 34-35 -1.0485943335308363 2216 36-37 -1.1741487181146937 2216 38-39 -1.0275222272345985 2216 40-41 -1.0133538938409146 2216 42-43 -1.1169144892058185 2216 44-45 -1.2521530170802961 2216 46-47 -1.2238356291179215 2216 48-49 -1.3826137061561425 2216 50-51 -1.2581522782064596 2216 52-53 -1.152132131819073 2216 54-55 -1.180604697535692 2216 56-57 -1.2280196455134558 2216 58-59 -1.2637604784943193 2216 60-61 -1.2166218272297868 2216 62-63 -1.1022044307873173 2216 64-65 -1.2497776750081613 2216 66-67 -1.1230284673202817 2216 68-69 -1.083476533711007 2216 70-71 -1.2375196118853893 2216 72-73 -1.1664982081693935 2216 74-75 -1.1521742720195505 2216 76-77 -1.1160020813211986 2216 78-79 -1.3887462823653607 2216 80-81 -1.5231104029089835 2216 82-83 -1.3890331302423249 2216 84-85 -1.4844250422284908 2216 86-87 -1.3381748944359622 2216 88-89 -1.6691841589632972 2216 90-91 -1.4014354100043178 2216 92-93 -1.48219641058893 2216 94-95 -1.4764612305870166 2216 96-97 -1.6189183684090231 2216 98-99 -1.81629013690306 2216 100 -1.7444517349623005 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 227.0 3 21.0 4 29.0 5 93.0 6 233.0 7 604.0 8 1350.0 9 2438.0 10 3558.0 11 5162.0 12 7362.0 13 10081.0 14 12399.0 15 16310.0 16 20111.0 17 27259.0 18 26476.0 19 14508.0 20 11089.0 21 10783.0 22 11337.0 23 12544.0 24 14861.0 25 18485.0 26 23597.0 27 30391.0 28 39368.0 29 51784.0 30 67478.0 31 86732.0 32 112113.0 33 145720.0 34 198980.0 35 290431.0 36 466751.0 37 844599.0 38 1224136.0 39 508599.0 40 1744.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.322911386043806 20.74469895904811 15.092656042622776 22.83973361228531 2 15.376053621708513 20.882330268259015 40.08328736223428 23.65832874779819 3 17.78223843409203 25.696945396983107 31.233409024564658 25.28740714436021 4 12.484539010769852 17.696978732299584 38.70151534477861 31.11696691215195 5 12.432799821656056 37.82099999930551 35.912946672984944 13.833253506053486 6 29.990835102921633 38.872150495990155 17.279639089640288 13.857375311447925 7 25.809151146260323 33.426687652483025 22.714430001970022 18.049731199286626 8 24.494869932812914 36.500414725090394 20.460704826622226 18.544010515474465 9 24.451940775180375 16.71705932505707 21.86053661062707 36.97046328913549 10-11 23.490297918186336 27.255440890812256 28.96193130007966 20.29232989092175 12-13 24.241494700971565 24.838791931293855 29.33923161598076 21.580481751753815 14-15 21.497562873308187 26.797027181159226 27.117359071619727 24.588050873912852 16-17 20.39182891109829 30.179646124740074 27.808439970669546 21.62008499349209 18-19 20.199609258845356 28.362717242555945 30.994727807081883 20.442945691516815 20-21 21.294311544871736 27.50258956527597 30.890420385206006 20.312678504646286 22-23 20.88319837545891 27.40019487270423 30.960175177088082 20.756431574748778 24-25 20.875953587925935 27.467522335845366 30.881004820199742 20.775519256028954 26-27 20.623827041402272 27.50946295597952 30.925977240754595 20.94073276186362 28-29 20.691111203966827 27.445627608890334 31.080014477775148 20.783246709367692 30-31 20.997406558677216 27.802047482917384 30.64255211479016 20.557993843615233 32-33 20.698270008817673 27.560036696712505 31.005238055222396 20.736455239247427 34-35 20.850588426754488 27.343804799317738 30.951788985399347 20.853817788528428 36-37 21.15529433606984 27.621275266862188 30.399857491390105 20.823572905677867 38-39 20.975015206399014 27.82515795267178 30.456735986239874 20.74309085468933 40-41 20.915854792018493 27.60864960466325 30.525865591292057 20.9496300120262 42-43 20.91933041956423 27.65377154074801 30.34416582556277 21.082732214124988 44-45 21.091787891421944 27.7845078779211 30.116261869083978 21.007442361572977 46-47 21.169612330744982 27.937463351373776 29.6592456164597 21.233678701421542 48-49 21.23337126030597 27.9607982489545 29.42603719466954 21.379793296069984 50-51 21.3002017259444 27.97186948604563 29.258537933691102 21.469390854318863 52-53 21.210500735407056 28.09381124895581 29.23172157246893 21.463966443168204 54-55 21.319269624548888 28.37094781645168 29.021672336203924 21.28811022279551 56-57 21.210604208282994 28.120347642983255 29.044886947507464 21.624161201226283 58-59 21.05610583976457 27.763342101227217 29.438096643603924 21.742455415404294 60-61 21.271251887268985 28.094067829176517 29.133518406457437 21.50116187709706 62-63 21.072395362320584 28.155566695723444 29.14241683485915 21.62962110709682 64-65 21.073666687462765 27.896700724039903 29.261962666334547 21.76766992216279 66-67 21.06929704730375 27.910430381516775 29.138797118952585 21.88147545222689 68-69 21.354166847525203 28.06290149871246 28.939830998840304 21.64310065492203 70-71 21.32893953257414 27.905311047179538 29.000381390239767 21.765368030006556 72-73 21.160507725602418 28.039487968538523 29.07973490261061 21.720269403248444 74-75 21.32239924448483 28.051128069844637 28.88270173297854 21.743770952691985 76-77 21.3313162226168 28.08208189901637 28.734984524424096 21.851617353942743 78-79 21.383316778958992 28.09713900824462 28.85043731444333 21.669106898353053 80-81 21.321287847456254 27.946883833590707 28.83975038119615 21.892077937756888 82-83 21.138078223518818 28.066753933822262 28.954341699181573 21.840826143477344 84-85 21.24687877492871 27.913463644238913 28.7728451914035 22.06681238942888 86-87 21.30540748639135 27.995176955319355 28.768162812135532 21.931252746153763 88-89 21.150146608828756 27.913239890750614 28.80831075052701 22.128302749893624 90-91 21.064201200315168 27.8344334119553 28.847463391128215 22.253901996601318 92-93 21.10750046068782 28.00261967002714 28.67774725045768 22.21213261882736 94-95 20.949895591701047 27.807587774737357 28.848256080452266 22.39426055310933 96-97 21.034542669985957 27.764842550796665 28.833622191131376 22.366992588086003 98-99 20.96725540754336 27.8816579444367 28.726444075790102 22.424642572229832 100 21.059245129268835 27.732077376101934 28.725854692940217 22.482822801689014 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 515.0 1 428.5 2 740.0 3 1115.5 4 689.5 5 392.0 6 1016.0 7 2820.5 8 4828.0 9 5515.5 10 4128.5 11 2454.5 12 2147.0 13 2403.5 14 2877.0 15 3276.5 16 3757.0 17 4355.5 18 4958.5 19 5727.0 20 6863.5 21 8457.0 22 10679.5 23 13923.5 24 18478.5 25 24754.5 26 33396.5 27 44044.5 28 56270.5 29 70649.5 30 85453.0 31 98001.0 32 107166.0 33 116518.5 34 126344.5 35 132655.0 36 137887.5 37 144938.5 38 152096.0 39 155612.0 40 157025.5 41 157758.0 42 157295.0 43 155909.5 44 152726.0 45 149572.5 46 145882.0 47 140277.5 48 134460.0 49 129198.5 50 125283.0 51 119751.0 52 110665.0 53 102572.5 54 96390.5 55 91670.5 56 85590.0 57 77418.5 58 70256.0 59 63655.0 60 55777.0 61 47242.0 62 40115.0 63 33725.5 64 27498.5 65 22080.5 66 17750.5 67 13752.5 68 10419.5 69 8047.5 70 6045.5 71 4416.0 72 3275.5 73 2469.0 74 1776.5 75 1244.5 76 912.5 77 639.0 78 433.0 79 300.0 80 184.0 81 85.5 82 47.0 83 26.5 84 19.5 85 10.5 86 5.0 87 1.5 88 1.0 89 1.5 90 1.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03879860445401497 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 3.2409335462781004E-4 9 0.0 10-11 0.0 12-13 0.04342850952012654 14-15 1.0417286398751038E-4 16-17 1.1574762665278929E-5 18-19 8.102333865695251E-5 20-21 5.787381332639465E-5 22-23 0.0 24-25 1.157476266527893E-4 26-27 9.259810132223143E-5 28-29 4.282662186153204E-4 30-31 0.0 32-33 2.314952533055786E-4 34-35 9.259810132223143E-5 36-37 9.259810132223143E-5 38-39 3.5881764262364683E-4 40-41 1.8519620264446286E-4 42-43 5.324390826028307E-4 44-45 2.777943039666943E-4 46-47 9.259810132223143E-5 48-49 8.796819625611986E-4 50-51 4.861400319417151E-4 52-53 6.134624212597833E-4 54-55 4.514157439458783E-4 56-57 5.555886079333886E-4 58-59 1.273223893180682E-4 60-61 2.0834572797502076E-4 62-63 9.144062505570355E-4 64-65 5.208643199375518E-4 66-67 0.001481569621155703 68-69 0.0015857424851432136 70-71 4.745652692764361E-4 72-73 0.0010996024532014983 74-75 9.607053012181513E-4 76-77 7.639343359084095E-4 78-79 0.003229358783612821 80-81 0.002696919701009991 82-83 0.0013310977065070769 84-85 0.0028936906663197324 86-87 0.001504719146486261 88-89 0.001967709653097418 90-91 0.0029515644796461273 92-93 0.0026621954130141537 94-95 0.0037733726288809314 96-97 0.003321956884935053 98-99 0.002986288767641964 100 0.0014584200958251451 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4319743.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.888490233770554 #Duplication Level Percentage of deduplicated Percentage of total 1 77.64314648674262 48.828602595507306 2 13.623890538773617 17.135718141872474 3 3.9687505112672774 7.487661833043122 4 1.6852440586826365 4.239298181039314 5 0.8536158787145658 2.6841306925966224 6 0.5314163543710128 2.005198332715643 7 0.33520378547398694 1.4756321992372625 8 0.2390060679180681 1.2024584614461864 9 0.1847018987605924 1.0454061200727953 >10 0.8815000080008687 9.573378173865153 >50 0.03902067679313455 1.6740266974430442 >100 0.013656554257304283 1.4374591330180653 >500 4.7884104302424165E-4 0.22017666549351725 >1k 2.5783744090256106E-4 0.26261362673360866 >5k 3.68339201289202E-5 0.15974098702958472 >10k+ 7.36678402578404E-5 0.5684981588863208 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12491 0.28916072090399825 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12051 0.27897492975855276 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6896 0.159639126679527 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.314952533055786E-5 0.0 4 0.0 0.0 0.0 2.314952533055786E-5 0.0 5 0.0 2.314952533055786E-5 0.0 2.314952533055786E-5 0.0 6 0.0 2.314952533055786E-5 0.0 2.314952533055786E-5 0.0 7 0.0 2.314952533055786E-5 0.0 4.629905066111572E-5 0.0 8 2.314952533055786E-5 2.314952533055786E-5 0.0 4.629905066111572E-5 0.0 9 2.314952533055786E-5 2.314952533055786E-5 0.0 4.629905066111572E-5 0.0 10-11 4.629905066111572E-5 2.314952533055786E-5 0.0 4.629905066111572E-5 0.0 12-13 5.787381332639465E-5 2.314952533055786E-5 0.0 8.102333865695251E-5 0.0 14-15 6.944857599167358E-5 4.629905066111572E-5 0.0 9.259810132223144E-5 0.0 16-17 6.944857599167358E-5 6.944857599167358E-5 0.0 1.157476266527893E-4 0.0 18-19 6.944857599167358E-5 6.944857599167358E-5 0.0 1.504719146486261E-4 0.0 20-21 8.102333865695251E-5 6.944857599167358E-5 0.0 2.777943039666943E-4 0.0 22-23 9.259810132223144E-5 6.944857599167358E-5 0.0 5.787381332639465E-4 0.0 24-25 9.259810132223144E-5 9.259810132223144E-5 0.0 0.0010996024532014983 0.0 26-27 1.0417286398751037E-4 1.157476266527893E-4 0.0 0.001990859178427976 0.0 28-29 1.3889715198334715E-4 1.157476266527893E-4 0.0 0.0049424236580741035 0.0 30-31 1.3889715198334715E-4 1.157476266527893E-4 0.0 0.013287827539740211 0.0 32-33 1.6204667731390502E-4 1.3889715198334715E-4 0.0 0.03291862502005327 0.0 34-35 1.851962026444629E-4 1.3889715198334715E-4 0.0 0.067017875831965 0.0 36-37 1.851962026444629E-4 1.504719146486261E-4 0.0 0.11739124295125891 0.0 38-39 1.851962026444629E-4 1.7362143997918394E-4 0.0 0.18099456379696663 0.0 40-41 1.851962026444629E-4 2.0834572797502073E-4 0.0 0.24994542499403322 0.0 42-43 1.851962026444629E-4 2.0834572797502073E-4 0.0 0.31941715051103736 0.0 44-45 2.0834572797502073E-4 2.0834572797502073E-4 0.0 0.4053134642500723 0.0 46-47 2.314952533055786E-4 2.0834572797502073E-4 0.0 0.5073218476191754 0.0 48-49 2.314952533055786E-4 2.0834572797502073E-4 0.0 0.6128026597878624 0.0 50-51 2.5464477863613647E-4 2.777943039666943E-4 0.0 0.7168713509113853 0.0 52-53 2.777943039666943E-4 2.777943039666943E-4 0.0 0.8173287160833411 0.0 54-55 2.777943039666943E-4 2.777943039666943E-4 0.0 0.930657217339087 0.0 56-57 2.777943039666943E-4 2.777943039666943E-4 0.0 1.0655842257282435 0.0 58-59 2.777943039666943E-4 2.777943039666943E-4 0.0 1.2070394002606175 0.0 60-61 2.777943039666943E-4 2.777943039666943E-4 0.0 1.3529392836564582 0.0 62-63 2.777943039666943E-4 2.777943039666943E-4 0.0 1.498040508428395 0.0 64-65 3.0094382929725215E-4 2.777943039666943E-4 0.0 1.6494731283782391 0.0 66-67 3.0094382929725215E-4 3.0094382929725215E-4 0.0 1.82587251139709 0.0 68-69 3.0094382929725215E-4 3.0094382929725215E-4 0.0 2.019738211277847 0.0 70-71 3.0094382929725215E-4 3.0094382929725215E-4 0.0 2.225687963381155 0.0 72-73 3.0094382929725215E-4 3.5881764262364683E-4 0.0 2.431348346417831 0.0 74-75 3.0094382929725215E-4 3.703924052889258E-4 0.0 2.6403538358647722 0.0 76-77 3.125185919625311E-4 3.703924052889258E-4 0.0 2.8665478478696533 0.0 78-79 3.35668117293089E-4 3.703924052889258E-4 0.0 3.119086019700709 0.0 80-81 3.472428799583679E-4 3.703924052889258E-4 0.0 3.3904331808628427 0.0 82-83 3.472428799583679E-4 3.703924052889258E-4 0.0 3.6688409472507972 0.0 84-85 3.472428799583679E-4 3.935419306194836E-4 0.0 3.941982196626049 0.0 86-87 3.472428799583679E-4 4.5141574394587825E-4 0.0 4.2334347205377725 0.0 88 3.472428799583679E-4 5.092895572722729E-4 0.0 4.470775228989317 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7430 0.0 58.469643 1 GTATCAA 13945 0.0 41.166542 1 ATCAACG 17310 0.0 32.82316 3 TCAACGC 17370 0.0 32.709785 4 CAACGCA 17985 0.0 31.591265 5 TATCAAC 18150 0.0 31.407642 2 AACGCAG 18770 0.0 30.400818 6 TGGTATC 3295 0.0 30.093882 2 GTGGTAT 3460 0.0 29.75879 1 ACGCAGA 21405 0.0 26.43864 7 CGCAGAG 21480 0.0 26.280624 8 GCAGAGT 23625 0.0 23.476358 9 GTACATG 10840 0.0 23.030985 1 TACATGG 10945 0.0 21.726307 2 GAGTACT 17480 0.0 21.397778 12-13 CAGAGTA 22615 0.0 21.217405 10-11 AGAGTAC 21525 0.0 20.707363 10-11 ACATGGG 11105 0.0 20.566902 3 GTACTTT 18990 0.0 19.513172 14-15 AGTACTT 18165 0.0 19.42608 12-13 >>END_MODULE