FastQCFastQC Report
Wed 25 May 2016
SRR2049358_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049358_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4934168
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48450.042.885631
GTATCAA79300.034.145431
TCAACGC84850.030.6925134
ATCAACG86200.030.0482273
CAACGCA91850.028.3539735
AACGCAG95950.027.040846
TATCAAC97750.027.0267662
GTACATG135500.026.1933461
TACATGG140450.024.4664042
ACGCAGA111000.022.950817
ACATGGG144550.022.861873
CGCAGAG112350.022.382188
GTGGTAT30400.020.102641
CATGGGG106900.019.700394
GCAGAGT127900.018.8157319
GTATAGG15150.017.0660651
GAGTACA101400.017.0605561
GTATAGA24400.016.9541551
CAGAGTA120600.016.75879910-11
AGAGTAC112400.016.72689610-11
Produced by FastQC (version 0.11.5)