##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049358_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4934168
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.648814551916352	31.0	31.0	34.0	28.0	34.0
2	30.85655372901774	31.0	31.0	34.0	28.0	34.0
3	30.92436272944091	31.0	31.0	34.0	28.0	34.0
4	34.64560894562163	37.0	35.0	37.0	32.0	37.0
5	34.65774594622639	37.0	35.0	37.0	32.0	37.0
6	34.79811510268803	37.0	35.0	37.0	32.0	37.0
7	34.78012990234625	37.0	35.0	37.0	32.0	37.0
8	34.72609526063969	37.0	35.0	37.0	32.0	37.0
9	36.36548471799096	39.0	37.0	39.0	32.0	39.0
10-11	36.31138907309196	39.0	37.0	39.0	32.0	39.0
12-13	36.25429170632212	39.0	37.0	39.0	32.0	39.0
14-15	37.46672397453836	40.0	38.0	41.0	32.0	41.0
16-17	37.432008294002145	40.0	38.0	41.0	32.0	41.0
18-19	37.44838977918871	40.0	38.0	41.0	32.0	41.0
20-21	37.5192227950082	40.0	38.0	41.0	32.0	41.0
22-23	37.4721429225758	40.0	38.0	41.0	32.0	41.0
24-25	37.4735223851316	40.0	38.0	41.0	32.0	41.0
26-27	37.45328412003807	40.0	38.0	41.0	32.0	41.0
28-29	37.39876712750761	40.0	38.0	41.0	32.0	41.0
30-31	37.34063311180324	40.0	37.5	41.0	32.0	41.0
32-33	37.28674175666495	40.0	37.0	41.0	31.0	41.0
34-35	37.197691282501935	40.0	37.0	41.0	31.0	41.0
36-37	37.11599453038486	40.0	37.0	41.0	31.0	41.0
38-39	37.01155422758204	40.0	37.0	41.0	30.5	41.0
40-41	36.9087807711452	40.0	37.0	41.0	30.0	41.0
42-43	36.770100247904004	40.0	37.0	41.0	30.0	41.0
44-45	36.640284542399044	40.0	36.5	41.0	30.0	41.0
46-47	36.519512712173565	40.0	36.0	41.0	30.0	41.0
48-49	36.395069644973574	40.0	36.0	41.0	30.0	41.0
50-51	36.253882518795464	39.5	36.0	41.0	29.5	41.0
52-53	36.10143331155324	39.0	35.0	41.0	29.0	41.0
54-55	35.96019987564266	39.0	35.0	41.0	29.0	41.0
56-57	35.82422436771509	39.0	35.0	41.0	29.0	41.0
58-59	35.62323658213502	39.0	35.0	41.0	28.0	41.0
60-61	35.39851267731459	38.5	35.0	40.0	28.0	41.0
62-63	35.17839116949402	38.0	34.5	40.0	28.0	41.0
64-65	34.927218631388314	38.0	34.0	40.0	28.0	41.0
66-67	34.66048095646521	37.0	34.0	40.0	27.5	41.0
68-69	34.36795038190836	37.0	34.0	40.0	27.0	41.0
70-71	34.07825959716005	36.0	34.0	39.0	26.0	41.0
72-73	33.729682896893664	36.0	34.0	39.0	26.0	41.0
74-75	33.37892751118325	35.0	33.0	39.0	26.0	40.0
76-77	33.045713988660296	35.0	33.0	37.5	26.0	40.0
78-79	32.72738331974104	35.0	33.0	37.0	25.5	39.0
80-81	32.37751876304171	35.0	33.0	37.0	25.0	39.0
82-83	32.05880930685781	35.0	33.0	36.0	24.5	38.5
84-85	31.765526528484642	35.0	32.5	36.0	24.0	37.0
86-87	31.449205418218433	35.0	32.0	35.5	22.5	37.0
88-89	31.195577653618603	35.0	32.0	35.0	21.5	36.5
90-91	30.98800973132654	35.0	32.0	35.0	20.0	36.0
92-93	30.74603813652069	35.0	32.0	35.0	19.0	36.0
94-95	30.538218094722353	35.0	32.0	35.0	17.0	36.0
96-97	30.27823120331533	35.0	32.0	35.0	6.0	35.5
98-99	30.064360090698166	35.0	32.0	35.0	2.0	35.0
100	29.935542729797607	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1559609828914823
1101	2	0.20764357911292564
1101	3	0.08015582526380172
1101	4	0.13166192779358
1101	5	0.15412127419021715
1101	6	0.29849779597756765
1101	7	0.3003067662156127
1101	8	0.34427240298636264
1101	9	0.40310254860166594
1101	10-11	0.42660353556100006
1101	12-13	0.4289224978729109
1101	14-15	0.5254216732974939
1101	16-17	0.4815986651149444
1101	18-19	0.4511442345218555
1101	20-21	0.3876998838117771
1101	22-23	0.43913676791307665
1101	24-25	0.5046791334937311
1101	26-27	0.4026842869553562
1101	28-29	0.3885863676731205
1101	30-31	0.4192451690724681
1101	32-33	0.3729820514703448
1101	34-35	0.43557797154187483
1101	36-37	0.45706303057505693
1101	38-39	0.47553033132510336
1101	40-41	0.5573978293070496
1101	42-43	0.49952465787898603
1101	44-45	0.5204909709426815
1101	46-47	0.45518851755058876
1101	48-49	0.4483023539924602
1101	50-51	0.5014493593782063
1101	52-53	0.5272554525712607
1101	54-55	0.5198499347741503
1101	56-57	0.49437506701499956
1101	58-59	0.5241579140639914
1101	60-61	0.5636770491318543
1101	62-63	0.537851955230856
1101	64-65	0.4754055049626942
1101	66-67	0.5597170700685083
1101	68-69	0.49264835965091436
1101	70-71	0.5089279208741004
1101	72-73	0.5466943696643938
1101	74-75	0.5413515941226592
1101	76-77	0.5351825811703321
1101	78-79	0.49143309403601876
1101	80-81	0.5505270309073254
1101	82-83	0.5635122702848037
1101	84-85	0.47402173360050526
1101	86-87	0.560084004006633
1101	88-89	0.520135673746946
1101	90-91	0.5589018539389023
1101	92-93	0.58016698450189
1101	94-95	0.5131037935340608
1101	96-97	0.5895609535926383
1101	98-99	0.6753184174966833
1101	100	0.6701295409358607
1102	1	-0.004681335403823539
1102	2	0.07555263226317166
1102	3	0.05813694039616735
1102	4	0.12215269100414616
1102	5	0.023049579989717017
1102	6	0.2670761694607293
1102	7	0.30060328050467433
1102	8	0.30570951431867144
1102	9	0.4041183109376263
1102	10-11	0.3531707126061647
1102	12-13	0.37214214223271114
1102	14-15	0.41298938841909916
1102	16-17	0.4062538607181807
1102	18-19	0.3947533329081878
1102	20-21	0.34843978477292126
1102	22-23	0.3700242222741892
1102	24-25	0.45629967783487047
1102	26-27	0.37103033138737374
1102	28-29	0.3732817091844467
1102	30-31	0.35527495069817405
1102	32-33	0.407057675682438
1102	34-35	0.41250568171535207
1102	36-37	0.4525765169426563
1102	38-39	0.41504715914007306
1102	40-41	0.5027788907535253
1102	42-43	0.43679650401363546
1102	44-45	0.3884945669394071
1102	46-47	0.3389379516937012
1102	48-49	0.3226505684252956
1102	50-51	0.426833441974928
1102	52-53	0.4235528267761097
1102	54-55	0.4412101731739142
1102	56-57	0.37770943797247014
1102	58-59	0.495183443595252
1102	60-61	0.4394475662282673
1102	62-63	0.3864250786334864
1102	64-65	0.2996567860903099
1102	66-67	0.3675030012000846
1102	68-69	0.34436578859523337
1102	70-71	0.4251248495445168
1102	72-73	0.34950814659651996
1102	74-75	0.3359071883602667
1102	76-77	0.3242391868276755
1102	78-79	0.35949732407981116
1102	80-81	0.2871554783729593
1102	82-83	0.31798023655473173
1102	84-85	0.3161868447373557
1102	86-87	0.26646117570188466
1102	88-89	0.28647955272365877
1102	90-91	0.3398200230569195
1102	92-93	0.2732419026154851
1102	94-95	0.2777507106012145
1102	96-97	0.2571161417363541
1102	98-99	0.23068328875972455
1102	100	0.21795060734670102
1103	1	0.034671153837358304
1103	2	0.19225794230007054
1103	3	0.08056100807982602
1103	4	0.13055741769214535
1103	5	0.15060650176446444
1103	6	0.2525744708529203
1103	7	0.36950409389802985
1103	8	0.2960549732359752
1103	9	0.39005125690186304
1103	10-11	0.38834685665207047
1103	12-13	0.3893904455310704
1103	14-15	0.41940193847357676
1103	16-17	0.40186817161179533
1103	18-19	0.43890055035364384
1103	20-21	0.43085435386717563
1103	22-23	0.3512346973852303
1103	24-25	0.372107542531694
1103	26-27	0.33010189693867886
1103	28-29	0.3447935263367583
1103	30-31	0.42968632545549923
1103	32-33	0.44931694629862307
1103	34-35	0.41235629352308223
1103	36-37	0.39377164920642826
1103	38-39	0.41098302212394344
1103	40-41	0.3800011531078056
1103	42-43	0.3917512870651123
1103	44-45	0.3075851005857828
1103	46-47	0.3802990459462876
1103	48-49	0.3893946855921655
1103	50-51	0.31325386664613575
1103	52-53	0.3400863502174758
1103	54-55	0.35007940635146895
1103	56-57	0.31928102769472133
1103	58-59	0.4025439189145956
1103	60-61	0.34061452343092924
1103	62-63	0.29043748591042373
1103	64-65	0.3611856596718894
1103	66-67	0.37952782707304067
1103	68-69	0.416671422609177
1103	70-71	0.3546986337521929
1103	72-73	0.41818817909798156
1103	74-75	0.31973451148815
1103	76-77	0.4630418245491654
1103	78-79	0.31505651815861313
1103	80-81	0.3219689404303594
1103	82-83	0.37187893430316166
1103	84-85	0.42780900635187535
1103	86-87	0.4073395075282349
1103	88-89	0.3259056159443041
1103	90-91	0.4063391539815804
1103	92-93	0.3355338114664441
1103	94-95	0.35452083491215447
1103	96-97	0.3196385391570473
1103	98-99	0.3140967464624289
1103	100	0.2493203198162881
1104	1	-0.20289030434436484
1104	2	-0.17357227122028718
1104	3	-0.18499706958550988
1104	4	-0.10151814686994243
1104	5	-0.080394176238066
1104	6	0.01560488386099479
1104	7	0.09626556563314637
1104	8	0.051964438301254745
1104	9	0.07887224454044883
1104	10-11	0.09090941355334792
1104	12-13	0.09111683380395874
1104	14-15	0.15207233949346488
1104	16-17	0.15768151950062048
1104	18-19	0.12880357445035173
1104	20-21	0.042780279366120055
1104	22-23	0.13714621763065082
1104	24-25	0.1268784740075546
1104	26-27	0.05876130647678224
1104	28-29	0.08120964282150567
1104	30-31	0.007113667059286399
1104	32-33	0.11193648401167167
1104	34-35	0.07954146639968229
1104	36-37	0.01846014364508619
1104	38-39	0.14639204675916062
1104	40-41	0.13157641247823193
1104	42-43	0.1333330297492452
1104	44-45	0.10349588330161907
1104	46-47	0.03513833932051824
1104	48-49	0.06554710605823288
1104	50-51	0.13628340171317177
1104	52-53	0.15639502682076767
1104	54-55	0.12150189733105066
1104	56-57	0.08627755594785214
1104	58-59	0.14531431837887965
1104	60-61	0.08854898794102439
1104	62-63	0.059803170927416716
1104	64-65	0.07478699485171347
1104	66-67	0.10481677996983052
1104	68-69	0.06793025185633184
1104	70-71	0.07300438972353618
1104	72-73	0.08273488236474691
1104	74-75	0.12188722031497434
1104	76-77	0.221871117894203
1104	78-79	0.13385958560181166
1104	80-81	0.11910752885415121
1104	82-83	0.06557871157735917
1104	84-85	0.1045109159216615
1104	86-87	0.14819017770282272
1104	88-89	0.18147856096461012
1104	90-91	0.2257449745015414
1104	92-93	0.17003181154559144
1104	94-95	0.19092796875947826
1104	96-97	0.12163152072793793
1104	98-99	0.16946516189082672
1104	100	0.1346349306875858
1105	1	-0.08576410762033504
1105	2	-0.016821777617227696
1105	3	0.007741007171244263
1105	4	0.07371307207445454
1105	5	0.03196370659016878
1105	6	0.07422401138550327
1105	7	0.1365873359844869
1105	8	0.1452282499223685
1105	9	0.1668538841361169
1105	10-11	0.1399815054742959
1105	12-13	0.16135469000161606
1105	14-15	0.18513741081117274
1105	16-17	0.1475867452056292
1105	18-19	0.07544500001021248
1105	20-21	0.15954610449718842
1105	22-23	0.10145919866864261
1105	24-25	0.0725284649936242
1105	26-27	0.0710878105022772
1105	28-29	0.13964620549496942
1105	30-31	0.119726625143592
1105	32-33	0.1539259671957467
1105	34-35	0.15426911497022644
1105	36-37	0.08873853646069563
1105	38-39	0.10767387394086114
1105	40-41	0.1764499012423002
1105	42-43	0.1464924765282305
1105	44-45	0.12142314962739675
1105	46-47	0.12223298439621288
1105	48-49	0.11078150490257599
1105	50-51	0.08078825972492609
1105	52-53	0.09134786160066
1105	54-55	0.04972902569637938
1105	56-57	0.031756113763037774
1105	58-59	0.0397243117004038
1105	60-61	0.0807776451159441
1105	62-63	0.006714210900533146
1105	64-65	0.04725605465128524
1105	66-67	0.13016594617167954
1105	68-69	0.06925141721276873
1105	70-71	0.05868385838734014
1105	72-73	0.09234170771426164
1105	74-75	0.12339435799948717
1105	76-77	0.2047424017558086
1105	78-79	0.11043701221053936
1105	80-81	0.15805430277406884
1105	82-83	0.13070872439317327
1105	84-85	0.1424530932256829
1105	86-87	0.11188909783450995
1105	88-89	0.03555866248694528
1105	90-91	0.048328732979424416
1105	92-93	0.10098609561551086
1105	94-95	0.07534868715909582
1105	96-97	0.08841259211180841
1105	98-99	0.15321120513467434
1105	100	0.14476131683555948
1106	1	-0.019500714687563203
1106	2	-0.011998797884256618
1106	3	-0.0067219959671582785
1106	4	0.033097660895002434
1106	5	0.027547971850289343
1106	6	0.10298450224424727
1106	7	0.11724470018477717
1106	8	0.12064624230921339
1106	9	0.17197955088415284
1106	10-11	0.18506085151065577
1106	12-13	0.19212061468154928
1106	14-15	0.23926801677811227
1106	16-17	0.28416268803949407
1106	18-19	0.2138966598577028
1106	20-21	0.1645032096908423
1106	22-23	0.24358658173658654
1106	24-25	0.1937118297637852
1106	26-27	0.20411555995358555
1106	28-29	0.271564475731104
1106	30-31	0.18643119581298606
1106	32-33	0.24519900709468345
1106	34-35	0.30502328625035346
1106	36-37	0.19708468439482374
1106	38-39	0.27022257220097856
1106	40-41	0.2581081373145011
1106	42-43	0.27027914228956007
1106	44-45	0.23619786016287492
1106	46-47	0.298244181889153
1106	48-49	0.2667571356732239
1106	50-51	0.21401009405510507
1106	52-53	0.27421901881725574
1106	54-55	0.31487268842760585
1106	56-57	0.26231088999368524
1106	58-59	0.3327708347241227
1106	60-61	0.29615341996277067
1106	62-63	0.25063113743474474
1106	64-65	0.2495212154192501
1106	66-67	0.24448627904651943
1106	68-69	0.22730376660138063
1106	70-71	0.240925402660082
1106	72-73	0.33724201557016187
1106	74-75	0.33895432429099515
1106	76-77	0.36206941488568134
1106	78-79	0.25988674287307845
1106	80-81	0.3223996920727217
1106	82-83	0.2944362513706622
1106	84-85	0.29065048001216454
1106	86-87	0.31469660972121005
1106	88-89	0.3385649664443484
1106	90-91	0.33621045811941386
1106	92-93	0.2471650829749663
1106	94-95	0.29412893156093745
1106	96-97	0.27091915892213336
1106	98-99	0.2910044916515844
1106	100	0.25728010741773133
1107	1	-0.021782226742072197
1107	2	-0.025647743114863175
1107	3	-0.04709394958351254
1107	4	0.035996638882423326
1107	5	0.03416812384190138
1107	6	0.07664229181447979
1107	7	0.06419995437764925
1107	8	0.1533531015859424
1107	9	0.17625022043342398
1107	10-11	0.15180531560918809
1107	12-13	0.11916095469727139
1107	14-15	0.13961523900033512
1107	16-17	0.18820160313383383
1107	18-19	0.19142589531367804
1107	20-21	0.19184573131033744
1107	22-23	0.16614578967683968
1107	24-25	0.20670604818620575
1107	26-27	0.12013568901543437
1107	28-29	0.18315670253927863
1107	30-31	0.14916499756874657
1107	32-33	0.16574285208943706
1107	34-35	0.16267118326293684
1107	36-37	0.12660266788539332
1107	38-39	0.23590694392530054
1107	40-41	0.2158006654853537
1107	42-43	0.17587236818054208
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2216	64-65	-1.9038003210614107
2216	66-67	-1.5870830349866552
2216	68-69	-2.018201268224246
2216	70-71	-1.754603488101381
2216	72-73	-2.183651287590827
2216	74-75	-2.647265955665585
2216	76-77	-3.2679187284238154
2216	78-79	-2.1989538392856716
2216	80-81	-2.028174082342872
2216	82-83	-2.3279262817689386
2216	84-85	-2.423423439774165
2216	86-87	-2.0018809601728975
2216	88-89	-1.9696620931386484
2216	90-91	-2.14168200590397
2216	92-93	-2.1577221103913438
2216	94-95	-2.0158712725916637
2216	96-97	-2.3252904223819897
2216	98-99	-2.0341448917371565
2216	100	-1.6673148960528614
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	70007.0
3	11582.0
4	7757.0
5	6644.0
6	6223.0
7	6455.0
8	7083.0
9	8258.0
10	9226.0
11	10520.0
12	11688.0
13	12931.0
14	14371.0
15	15147.0
16	15497.0
17	16121.0
18	15235.0
19	14221.0
20	14175.0
21	15158.0
22	16972.0
23	20206.0
24	24748.0
25	31037.0
26	38222.0
27	44837.0
28	55016.0
29	69378.0
30	84569.0
31	102337.0
32	130454.0
33	174637.0
34	242007.0
35	352862.0
36	583924.0
37	1003737.0
38	1248946.0
39	420990.0
40	990.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.31920772203057	20.300653427845955	14.049904723218976	23.330234126904497
2	15.617048849033202	20.204757786273873	40.20158695596641	23.976606408726514
3	18.51807023732772	24.97062293965234	30.23264442200786	26.27866240101208
4	12.717242682479798	16.706377912653643	38.590734655347475	31.98564474951909
5	13.284439063862555	37.70652043667058	35.45960484893066	13.549435650536207
6	31.2608510970468	37.574808322207254	17.619860080026683	13.544480500719267
7	27.151592687283436	32.43695042607502	22.41594572840277	17.99551115823877
8	24.90741058002023	36.37649604369925	20.490915014803562	18.225178361476964
9	24.785847549001122	16.940335758603506	21.535778716094978	36.7380379763004
10-11	23.93667870251293	26.971808383704587	28.92519196765913	20.16632094612335
12-13	24.21785448935146	24.4763723439726	28.928219908764007	22.377553257911934
14-15	21.69250599499112	26.707283755816952	26.03190131061074	25.56830893858118
16-17	21.364942148597805	30.021673938449904	26.70903125371585	21.90435265923644
18-19	21.334320980345424	29.050842093429154	28.549865006143733	21.06497192008169
20-21	22.391194689045427	28.26437221123793	28.142349931958666	21.202083167757976
22-23	22.031777016708826	28.139838580573063	28.18018532740878	21.648199075309332
24-25	21.914714062047793	28.234599432427267	28.091474599371608	21.759211906153332
26-27	21.68346172919401	28.164908294173912	28.213576265681507	21.938053710950566
28-29	21.70656744750329	28.235402763432383	28.271865887070145	21.78616390199418
30-31	21.93806699952163	28.53172156191987	27.925310273735075	21.60490116482342
32-33	21.655287962530917	28.335826886234482	28.275815368483393	21.733069782751212
34-35	21.804121877260883	28.042144287701586	28.319129768468155	21.834604066569373
36-37	22.005459497206843	28.364469132370964	27.863668879842102	21.76640249058009
38-39	21.860995297511707	28.466192058988405	27.96818851311557	21.704624130384317
40-41	21.759336976161052	28.206460829059584	28.16891342850748	21.86528876627189
42-43	21.74418348869744	28.218578390779992	28.099823910877475	21.937414209645095
44-45	21.812215071560733	28.249087656261135	28.068326971264103	21.870370300914033
46-47	21.795361349294435	28.370268544552207	27.96028653429318	21.874083571860183
48-49	21.73094872978016	28.284753145037882	27.947218588749145	22.03707953643281
50-51	21.77559014889861	28.191992107830455	27.95705976947923	22.075357973791704
52-53	21.79049562636703	28.146548914632884	28.030288362459633	22.032667096540457
54-55	21.823507909691912	28.43378591718225	27.864773452288734	21.87793272083711
56-57	21.732731969892363	28.26588943814065	27.85554545714414	22.145833134822848
58-59	21.64461376023511	27.935247049588323	28.15614779589874	22.263991394277827
60-61	21.8503325810865	28.235246773148976	27.869908839361496	22.044511806403026
62-63	21.75671638780276	28.2681338745416	27.831047004451154	22.14410273320448
64-65	21.798619169604216	28.034473726676996	27.955632268355323	22.211274835363465
66-67	21.684757410494555	28.099728887188647	27.83199155501737	22.383522147299423
68-69	21.97302894112183	28.175721708512384	27.72683929222891	22.124410058136874
70-71	21.935047595858464	28.029863845444503	27.81441614168986	22.22067241700717
72-73	21.7801316506897	28.13286179738801	27.881334963415895	22.205671588506398
74-75	21.893692415576297	28.12061024411021	27.786418616041807	22.199278724271686
76-77	21.879684683456425	28.098901792866908	27.73412685178831	22.287286671888356
78-79	21.924191252599798	28.156577559447893	27.75925136393668	22.159979824015625
80-81	21.868013302550303	28.05359985468075	27.795824423203396	22.28256241956555
82-83	21.774892068894665	28.08203605336309	27.899649692088076	22.243422185654172
84-85	21.83881189720601	27.980517793493842	27.799518043564618	22.38115226573553
86-87	21.973782736228102	28.080787991784035	27.732836880101214	22.212592391886655
88-89	21.876021591625687	27.94938855793051	27.808153594872277	22.36643625557152
90-91	21.870319035199586	27.840991164092117	27.801437534205224	22.48725226650307
92-93	21.974351972308433	27.98739250390411	27.678053919001684	22.36020160478577
94-95	21.873824771877768	27.828773456666354	27.785337532105586	22.51206423935029
96-97	21.9109483098265	27.790156314093885	27.789973912521827	22.508921463557787
98-99	21.935420520744326	27.852882188040617	27.714186059331585	22.497511231883472
100	21.986644962230713	27.753412530744797	27.69305787723482	22.566884629789662
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1783.0
1	1403.5
2	1209.5
3	1470.0
4	1434.5
5	1199.5
6	1024.0
7	924.5
8	914.5
9	930.5
10	809.5
11	720.0
12	730.0
13	798.0
14	915.0
15	997.0
16	1172.5
17	1547.0
18	2037.0
19	2775.5
20	4008.5
21	5794.0
22	8316.0
23	12013.5
24	17194.0
25	24033.5
26	33210.0
27	44333.5
28	57622.5
29	72957.5
30	87609.0
31	100013.0
32	110604.5
33	120726.0
34	131085.5
35	139628.0
36	147697.5
37	157156.5
38	164897.5
39	169524.5
40	173025.0
41	175502.0
42	175615.0
43	174911.0
44	172692.0
45	169680.0
46	167327.0
47	162482.5
48	156981.0
49	153348.5
50	150204.0
51	145011.0
52	137373.5
53	131114.5
54	125874.5
55	119322.0
56	110906.0
57	101087.5
58	92546.0
59	85495.0
60	76085.5
61	64817.5
62	55708.0
63	47665.5
64	39105.5
65	31407.5
66	24979.5
67	19500.0
68	15114.0
69	11939.0
70	9212.0
71	6893.5
72	5248.0
73	3861.0
74	2824.0
75	2047.0
76	1397.5
77	988.5
78	708.5
79	501.0
80	338.5
81	214.5
82	152.0
83	116.0
84	94.5
85	74.0
86	51.0
87	40.0
88	36.0
89	29.5
90	23.5
91	23.0
92	20.5
93	16.5
94	11.0
95	11.0
96	13.0
97	11.5
98	12.0
99	9.5
100	22.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013112646346861314
2	0.034575231325727054
3	0.03542643866199935
4	0.0384259311802922
5	0.041121421078487805
6	0.041810493684041564
7	0.039277138516564496
8	0.03868940011770981
9	0.03919607115120523
10-11	0.039662208502021006
12-13	0.040807285037720645
14-15	0.03902380299981679
16-17	0.03539603839998962
18-19	0.03863873301436027
20-21	0.0379395269881366
22-23	0.03899340273780706
24-25	0.035679774178747056
26-27	0.03579124180611604
28-29	0.0362269788949221
30-31	0.03626751257760173
32-33	0.0361053778468832
34-35	0.036064844164203566
36-37	0.033693623727445034
38-39	0.033906425561513104
40-41	0.035183236565921545
42-43	0.03490963420783403
44-45	0.03403816003022191
46-47	0.033126152169930166
48-49	0.0331565524319399
50-51	0.034129360816251085
52-53	0.03470696579443586
54-55	0.03394695924419274
56-57	0.03240667930236668
58-59	0.0331565524319399
60-61	0.03266001481911439
62-63	0.032923483756532
64-65	0.03007599254828778
66-67	0.03110960145661842
68-69	0.03211281010293934
70-71	0.0334098879486876
72-73	0.030268527541016034
74-75	0.030785331995181353
76-77	0.031160268559967962
78-79	0.031839207744851813
80-81	0.03202160931691017
82-83	0.030552263319773468
84-85	0.031707473276143004
86-87	0.031991209054900445
88-89	0.03257894745375512
90-91	0.032923483756532
92-93	0.03135280355269622
94-95	0.032730948763803745
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4934168.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.3393117484266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.50344342279362	50.508575197040884
2	13.012421064224696	16.74420430905891
3	3.8692839551628793	7.468411999032286
4	1.6237996533226484	4.178966084484516
5	0.831521173865416	2.6749750015372316
6	0.503208441386485	1.942565087088295
7	0.32893487761186324	1.4814410544920154
8	0.22147816762973613	1.13998022980799
9	0.1773842718372402	1.027150376850346
>10	0.8742117596543038	9.865480159735455
>50	0.04448320493741361	1.9261463318199472
>100	0.009735385444866506	0.9609927466709304
>500	6.30814196993385E-5	0.033625626328397364
>1k	3.1540709225548725E-5	0.04748579605291986
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	1.013342066990828E-5	0.0	0.0	1.013342066990828E-5	0.0
12-13	2.026684133981656E-5	0.0	0.0	6.080052401944968E-5	0.0
14-15	2.026684133981656E-5	1.013342066990828E-5	0.0	9.120078602917452E-5	0.0
16-17	2.026684133981656E-5	2.026684133981656E-5	0.0	1.013342066990828E-4	0.0
18-19	2.026684133981656E-5	2.026684133981656E-5	0.0	1.1146762736899109E-4	0.0
20-21	2.026684133981656E-5	2.026684133981656E-5	0.0	1.7226815138844076E-4	0.0
22-23	2.026684133981656E-5	2.026684133981656E-5	0.0	4.053368267963312E-4	0.0
24-25	2.026684133981656E-5	2.026684133981656E-5	0.0	8.30940494932479E-4	0.0
26-27	3.040026200972484E-5	2.026684133981656E-5	0.0	0.001702414672544591	0.0
28-29	4.053368267963312E-5	6.080052401944968E-5	0.0	0.005157911120983315	1.013342066990828E-5
30-31	4.053368267963312E-5	8.106736535926624E-5	0.0	0.015159597322182788	2.026684133981656E-5
32-33	4.053368267963312E-5	8.106736535926624E-5	0.0	0.03862859959369036	2.026684133981656E-5
34-35	4.053368267963312E-5	8.106736535926624E-5	0.0	0.07552438425282641	2.026684133981656E-5
36-37	4.053368267963312E-5	8.106736535926624E-5	0.0	0.134014488359537	2.026684133981656E-5
38-39	4.053368267963312E-5	1.2160104803889936E-4	0.0	0.21241676408261737	2.026684133981656E-5
40-41	4.053368267963312E-5	1.2160104803889936E-4	0.0	0.29228838580283445	2.026684133981656E-5
42-43	4.053368267963312E-5	1.2160104803889936E-4	0.0	0.3705791938985458	2.026684133981656E-5
44-45	4.053368267963312E-5	1.2160104803889936E-4	0.0	0.46600561634707205	2.026684133981656E-5
46-47	4.053368267963312E-5	1.2160104803889936E-4	0.0	0.5829148906158039	2.026684133981656E-5
48-49	5.06671033495414E-5	1.2160104803889936E-4	0.0	0.7021345847972749	2.026684133981656E-5
50-51	6.080052401944968E-5	1.2160104803889936E-4	0.0	0.8194086622101233	2.026684133981656E-5
52-53	6.080052401944968E-5	1.2160104803889936E-4	0.0	0.9347573896956893	2.026684133981656E-5
54-55	6.080052401944968E-5	1.418678893787159E-4	0.0	1.0601078033824547	2.026684133981656E-5
56-57	6.080052401944968E-5	1.418678893787159E-4	0.0	1.2073970728195715	2.026684133981656E-5
58-59	6.080052401944968E-5	1.418678893787159E-4	0.0	1.3651744326500435	2.026684133981656E-5
60-61	6.080052401944968E-5	1.418678893787159E-4	0.0	1.5295689161779658	2.026684133981656E-5
62-63	6.080052401944968E-5	1.418678893787159E-4	0.0	1.689950565120604	2.026684133981656E-5
64-65	6.080052401944968E-5	1.418678893787159E-4	0.0	1.8569493377606925	2.026684133981656E-5
66-67	6.080052401944968E-5	1.418678893787159E-4	0.0	2.048237919746551	2.026684133981656E-5
68-69	6.080052401944968E-5	1.7226815138844076E-4	0.0	2.2562973129411077	2.026684133981656E-5
70-71	6.080052401944968E-5	1.8240157205834905E-4	0.0	2.4741557239234657	2.026684133981656E-5
72-73	6.080052401944968E-5	2.026684133981656E-4	0.0	2.6948413592727283	4.053368267963312E-5
74-75	6.080052401944968E-5	2.026684133981656E-4	0.0	2.919296627111197	4.053368267963312E-5
76-77	6.080052401944968E-5	2.026684133981656E-4	0.0	3.159114160685246	4.053368267963312E-5
78-79	6.080052401944968E-5	2.026684133981656E-4	0.0	3.426403397695417	4.053368267963312E-5
80-81	6.080052401944968E-5	2.026684133981656E-4	0.0	3.708365827835615	4.053368267963312E-5
82-83	6.080052401944968E-5	2.026684133981656E-4	0.0	3.996854180887234	4.053368267963312E-5
84-85	6.080052401944968E-5	2.026684133981656E-4	0.0	4.283032114026113	4.053368267963312E-5
86-87	6.080052401944968E-5	2.026684133981656E-4	0.0	4.590247028475723	6.080052401944968E-5
88	6.080052401944968E-5	2.026684133981656E-4	0.0	4.835344074218795	6.080052401944968E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4845	0.0	42.88563	1
GTATCAA	7930	0.0	34.14543	1
TCAACGC	8485	0.0	30.692513	4
ATCAACG	8620	0.0	30.048227	3
CAACGCA	9185	0.0	28.353973	5
AACGCAG	9595	0.0	27.04084	6
TATCAAC	9775	0.0	27.026766	2
GTACATG	13550	0.0	26.193346	1
TACATGG	14045	0.0	24.466404	2
ACGCAGA	11100	0.0	22.95081	7
ACATGGG	14455	0.0	22.86187	3
CGCAGAG	11235	0.0	22.38218	8
GTGGTAT	3040	0.0	20.10264	1
CATGGGG	10690	0.0	19.70039	4
GCAGAGT	12790	0.0	18.815731	9
GTATAGG	1515	0.0	17.066065	1
GAGTACA	10140	0.0	17.060556	1
GTATAGA	2440	0.0	16.954155	1
CAGAGTA	12060	0.0	16.758799	10-11
AGAGTAC	11240	0.0	16.726896	10-11
>>END_MODULE