FastQCFastQC Report
Wed 25 May 2016
SRR2049358_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049358_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4934168
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121170.2455733165145573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113730.23049478655773376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69350.14055054469162787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79100.055.929121
GTATCAA139100.040.2202381
ATCAACG171800.032.279383
TCAACGC172500.032.2301254
CAACGCA177950.031.2430275
TATCAAC179400.030.9381122
AACGCAG186850.029.8521026
GTGGTAT36550.029.1986851
ACGCAGA217100.025.562637
CGCAGAG219600.025.1859478
TGGTATC39150.024.9688132
GTACATG127750.023.0376511
GCAGAGT242950.022.4166439
GAGTACT174100.020.84677912-13
TACATGG131500.020.8000132
CAGAGTA234200.020.45281410-11
AGAGTAC222250.020.12415710-11
ACATGGG129700.020.0741023
AGTACTT180700.018.86254512-13
GTACTTT192500.018.56651714-15
Produced by FastQC (version 0.11.5)