##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049358_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4934168
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.77654773813944	31.0	31.0	34.0	30.0	34.0
2	32.03097381361964	33.0	31.0	34.0	30.0	34.0
3	31.987366664450825	33.0	31.0	34.0	30.0	34.0
4	35.69922284770198	37.0	35.0	37.0	33.0	37.0
5	35.79841424126621	37.0	35.0	37.0	35.0	37.0
6	35.8526746555853	37.0	35.0	37.0	35.0	37.0
7	35.80429142258634	37.0	35.0	37.0	35.0	37.0
8	35.79119498971255	37.0	35.0	37.0	35.0	37.0
9	37.52936097838582	39.0	37.0	39.0	35.0	39.0
10-11	37.49036301560871	39.0	37.0	39.0	34.5	39.0
12-13	37.442347423111656	39.0	37.0	39.0	34.5	39.0
14-15	38.736288265823134	40.0	38.0	41.0	34.5	41.0
16-17	38.75987522111124	40.0	38.0	41.0	35.0	41.0
18-19	38.82582514417831	40.0	38.0	41.0	35.0	41.0
20-21	38.85282635289273	40.0	38.0	41.0	35.0	41.0
22-23	38.81991391456472	40.0	38.0	41.0	35.0	41.0
24-25	38.79844818822545	40.0	38.0	41.0	35.0	41.0
26-27	38.68487625472015	40.0	38.0	41.0	34.0	41.0
28-29	38.60145570641292	40.0	38.0	41.0	34.0	41.0
30-31	38.50279094672091	40.0	38.0	41.0	34.0	41.0
32-33	38.36603283471499	40.0	38.0	41.0	34.0	41.0
34-35	38.26446829536408	40.0	38.0	41.0	34.0	41.0
36-37	38.15405768510517	40.0	38.0	41.0	34.0	41.0
38-39	38.06152830629196	40.0	38.0	41.0	33.0	41.0
40-41	37.94820484426148	40.0	37.5	41.0	33.0	41.0
42-43	37.813095743801185	40.0	37.0	41.0	33.0	41.0
44-45	37.63931821940396	40.0	37.0	41.0	33.0	41.0
46-47	37.41155722707455	40.0	37.0	41.0	32.5	41.0
48-49	37.20699345867429	40.0	37.0	41.0	32.0	41.0
50-51	37.06192259769023	40.0	37.0	41.0	31.0	41.0
52-53	36.920018227997105	40.0	36.0	41.0	31.0	41.0
54-55	36.77388467518739	39.5	36.0	41.0	31.0	41.0
56-57	36.628076405181176	39.0	35.5	41.0	31.0	41.0
58-59	36.43685125840871	39.0	35.0	41.0	31.0	41.0
60-61	36.25462337318065	39.0	35.0	41.0	30.5	41.0
62-63	36.046718413317095	39.0	35.0	41.0	30.0	41.0
64-65	35.814007346324644	38.0	35.0	40.0	30.0	41.0
66-67	35.5421108482727	37.5	35.0	40.0	29.5	41.0
68-69	35.249757608577575	37.0	35.0	40.0	29.0	41.0
70-71	34.91414388403476	36.5	34.0	39.5	29.0	41.0
72-73	34.61835804942191	36.0	34.0	39.0	29.0	41.0
74-75	34.31431094360792	36.0	34.0	39.0	29.0	40.5
76-77	33.98937683110911	35.0	34.0	38.5	29.0	40.0
78-79	33.658524496936465	35.0	34.0	37.0	28.5	39.0
80-81	33.344650607762034	35.0	34.0	37.0	28.0	39.0
82-83	33.049787725103805	35.0	34.0	36.0	28.0	39.0
84-85	32.78349450201128	35.0	33.0	36.0	27.0	37.0
86-87	32.555680207889154	35.0	33.0	36.0	27.0	37.0
88-89	32.331582143129296	35.0	33.0	35.0	27.0	37.0
90-91	32.14232764267451	35.0	33.0	35.0	26.5	36.0
92-93	31.964613689683855	35.0	33.0	35.0	26.0	36.0
94-95	31.80572104557445	35.0	33.0	35.0	26.0	36.0
96-97	31.612089515395503	35.0	33.0	35.0	25.5	36.0
98-99	31.463940526548754	35.0	33.0	35.0	25.0	35.0
100	31.35134373211451	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2081716473103441
1101	2	0.20214646811378856
1101	3	0.3491850858313583
1101	4	0.21459822261270034
1101	5	0.16911622602101062
1101	6	0.2035086335132732
1101	7	0.08828846288717784
1101	8	0.10581124233407024
1101	9	0.19119169088981636
1101	10-11	0.24684560776665876
1101	12-13	0.14945196139362338
1101	14-15	0.3413672746596319
1101	16-17	0.24624467265684302
1101	18-19	0.3196355448751973
1101	20-21	0.31651105540823465
1101	22-23	0.2763560337648059
1101	24-25	0.23987968105723212
1101	26-27	0.29607812325782135
1101	28-29	0.32933179206323615
1101	30-31	0.31137792680009113
1101	32-33	0.32794757753719495
1101	34-35	0.3452449152911967
1101	36-37	0.3390954056136479
1101	38-39	0.2685424345307794
1101	40-41	0.3238426546705
1101	42-43	0.3633433210865462
1101	44-45	0.3278258412975603
1101	46-47	0.352524877646772
1101	48-49	0.4447889673979546
1101	50-51	0.41272520720369243
1101	52-53	0.4091805737522165
1101	54-55	0.3855756723382697
1101	56-57	0.3951074804633805
1101	58-59	0.43811687625187545
1101	60-61	0.3912879304713286
1101	62-63	0.41714905113416023
1101	64-65	0.3332941710119144
1101	66-67	0.26200592591432326
1101	68-69	0.30443165995753674
1101	70-71	0.32724245589811574
1101	72-73	0.30227946843518083
1101	74-75	0.32088140499519824
1101	76-77	0.237558744429478
1101	78-79	0.29058714444575884
1101	80-81	0.3930568655465265
1101	82-83	0.3395632460223794
1101	84-85	0.4088520018326207
1101	86-87	0.32363672832882884
1101	88-89	0.33324622204007426
1101	90-91	0.3939743367989763
1101	92-93	0.34194078925050775
1101	94-95	0.37294038836780175
1101	96-97	0.3947888590441728
1101	98-99	0.4679869956810663
1101	100	0.4727697494368073
1102	1	0.23464315730348062
1102	2	0.18990894271668424
1102	3	0.40604456200351535
1102	4	0.2149981069940452
1102	5	0.19096096048974687
1102	6	0.18182839020434471
1102	7	0.16373819990018745
1102	8	0.2167994709684251
1102	9	0.1370173614297272
1102	10-11	0.21677209446357892
1102	12-13	0.21466702435198215
1102	14-15	0.274253846539672
1102	16-17	0.19162068126606613
1102	18-19	0.28746311544426817
1102	20-21	0.22313279660215102
1102	22-23	0.2553147004196745
1102	24-25	0.2689939263975205
1102	26-27	0.3663726878416398
1102	28-29	0.2766401760960733
1102	30-31	0.25402465246820327
1102	32-33	0.31289933531984815
1102	34-35	0.2507024543719538
1102	36-37	0.31618421809623953
1102	38-39	0.31918777316462865
1102	40-41	0.30967729937405863
1102	42-43	0.37185785917482406
1102	44-45	0.3978637866848729
1102	46-47	0.2516676688795201
1102	48-49	0.1574564750407319
1102	50-51	0.25450936687951753
1102	52-53	0.28925765042166063
1102	54-55	0.23781541043165078
1102	56-57	0.33648890668818154
1102	58-59	0.28853391998737266
1102	60-61	0.3171055627385826
1102	62-63	0.33407377100202496
1102	64-65	0.28234101515586474
1102	66-67	0.24301109312021651
1102	68-69	0.2766507548770889
1102	70-71	0.30237072694058753
1102	72-73	0.35582876801452556
1102	74-75	0.32574067191089995
1102	76-77	0.2509842344796098
1102	78-79	0.35366698795959195
1102	80-81	0.3321339138876169
1102	82-83	0.37238217481926483
1102	84-85	0.35402133113483103
1102	86-87	0.3294956047352926
1102	88-89	0.31206925349729175
1102	90-91	0.26911907319143324
1102	92-93	0.36562004861577435
1102	94-95	0.33528467676638485
1102	96-97	0.3293380781839801
1102	98-99	0.34840456052844715
1102	100	0.4031341032406104
1103	1	0.14197366458899197
1103	2	0.18139075017468542
1103	3	0.32231694105036723
1103	4	0.18140120955216332
1103	5	0.16620756105354673
1103	6	0.15940200368265778
1103	7	0.18743130082254567
1103	8	0.15268843368457397
1103	9	0.24223373299215467
1103	10-11	0.2537610982192291
1103	12-13	0.201336712320483
1103	14-15	0.2941198232039852
1103	16-17	0.2749054041558807
1103	18-19	0.28040360958000576
1103	20-21	0.28841337325236793
1103	22-23	0.3035640995458593
1103	24-25	0.3203742874429665
1103	26-27	0.3188883284041424
1103	28-29	0.36435242543263513
1103	30-31	0.3389184131353531
1103	32-33	0.37561985583374025
1103	34-35	0.372092681876687
1103	36-37	0.3444723323849246
1103	38-39	0.34335918355816375
1103	40-41	0.3666835544985929
1103	42-43	0.36777513215213276
1103	44-45	0.4878188043713223
1103	46-47	0.41864251829064614
1103	48-49	0.37224151993461163
1103	50-51	0.37590049833805494
1103	52-53	0.38128653465418694
1103	54-55	0.42197833230591186
1103	56-57	0.4350034433265719
1103	58-59	0.4646582754191755
1103	60-61	0.43165390143261817
1103	62-63	0.4338303205207268
1103	64-65	0.4567661535924614
1103	66-67	0.39207242748651794
1103	68-69	0.4204838562349167
1103	70-71	0.48476398237615825
1103	72-73	0.4239953744045053
1103	74-75	0.4306092570714881
1103	76-77	0.39270310578874046
1103	78-79	0.4229850742909562
1103	80-81	0.45010053425350094
1103	82-83	0.49781981619469207
1103	84-85	0.4225287870637402
1103	86-87	0.42719945201771736
1103	88-89	0.4282714739379827
1103	90-91	0.47527821748203536
1103	92-93	0.5038066811197908
1103	94-95	0.5025288258074525
1103	96-97	0.5802426411777297
1103	98-99	0.6044335590521683
1103	100	0.6548995839801464
1104	1	0.1642625957959183
1104	2	0.2158600970815172
1104	3	0.3081070996195976
1104	4	0.18131221456148694
1104	5	0.15497669622361343
1104	6	0.1407473271187598
1104	7	0.15931496210713192
1104	8	0.16012866986253727
1104	9	0.18830590942608438
1104	10-11	0.20470239252675526
1104	12-13	0.18414296141312292
1104	14-15	0.2198831838794888
1104	16-17	0.22462446302526473
1104	18-19	0.223419693969646
1104	20-21	0.25228605354907074
1104	22-23	0.2308869325418499
1104	24-25	0.2322852000024369
1104	26-27	0.1864318109649119
1104	28-29	0.24311235293746591
1104	30-31	0.2897871738465767
1104	32-33	0.29416362952655817
1104	34-35	0.26030810081954314
1104	36-37	0.3312216489229769
1104	38-39	0.35511446665885416
1104	40-41	0.2929395468803051
1104	42-43	0.3876979365263651
1104	44-45	0.3358582042661098
1104	46-47	0.4050304046849291
1104	48-49	0.3952832042866561
1104	50-51	0.3683415960141332
1104	52-53	0.33320004888992827
1104	54-55	0.316347365951124
1104	56-57	0.3402399532877851
1104	58-59	0.41791461265879093
1104	60-61	0.3639155222440493
1104	62-63	0.3938794678519031
1104	64-65	0.31450858475972154
1104	66-67	0.3537317892477745
1104	68-69	0.3625208109752478
1104	70-71	0.32490582971263393
1104	72-73	0.3576326301574184
1104	74-75	0.3293953484015191
1104	76-77	0.26277544465654046
1104	78-79	0.3542744474613784
1104	80-81	0.34326989990526613
1104	82-83	0.3730908980401182
1104	84-85	0.39630611838239105
1104	86-87	0.32971731928167713
1104	88-89	0.3384790940597
1104	90-91	0.26576599612531027
1104	92-93	0.3164300468922292
1104	94-95	0.42043160278002034
1104	96-97	0.4160546060138266
1104	98-99	0.42067977103776144
1104	100	0.48186514744517694
1105	1	0.16552285029349179
1105	2	0.15841232951127893
1105	3	0.2790157556299704
1105	4	0.1699820709444495
1105	5	0.15538285689265763
1105	6	0.09746038084723807
1105	7	0.1662852300338713
1105	8	0.11471556385488668
1105	9	0.20524684356596623
1105	10-11	0.1909947921562889
1105	12-13	0.16447485391333316
1105	14-15	0.23896979571331656
1105	16-17	0.21050887466000034
1105	18-19	0.18955569287516028
1105	20-21	0.21414143150209242
1105	22-23	0.25067851253192686
1105	24-25	0.2658752787852521
1105	26-27	0.271404957309322
1105	28-29	0.27718602862901776
1105	30-31	0.32315605793509405
1105	32-33	0.33465353150368315
1105	34-35	0.33189367597703523
1105	36-37	0.27884680250882354
1105	38-39	0.2449183455223789
1105	40-41	0.23215283140797283
1105	42-43	0.2691794146729478
1105	44-45	0.24441264357573544
1105	46-47	0.2960448206609314
1105	48-49	0.29234798415031094
1105	50-51	0.26376078516278056
1105	52-53	0.31006024312635105
1105	54-55	0.3104578155585287
1105	56-57	0.23998482859681047
1105	58-59	0.3000277805501099
1105	60-61	0.2946535815926623
1105	62-63	0.3240361462941479
1105	64-65	0.31598575253614314
1105	66-67	0.3800823034863754
1105	68-69	0.315712581469775
1105	70-71	0.33285162476856556
1105	72-73	0.3622022775490379
1105	74-75	0.35434824974198165
1105	76-77	0.2824459091417353
1105	78-79	0.2929967286729749
1105	80-81	0.3040325458779165
1105	82-83	0.3140147890038918
1105	84-85	0.33681940836379454
1105	86-87	0.30609730643107724
1105	88-89	0.279186168848895
1105	90-91	0.3006566718151191
1105	92-93	0.3452405212012124
1105	94-95	0.35123227672601587
1105	96-97	0.44167301586717755
1105	98-99	0.4683514340367978
1105	100	0.506535139915357
1106	1	0.15915897686613079
1106	2	0.1895582488480656
1106	3	0.2670734606642782
1106	4	0.16510889505071447
1106	5	0.1861342017748555
1106	6	0.11370803801259655
1106	7	0.14130382274736064
1106	8	0.16852798309322026
1106	9	0.26052026267849726
1106	10-11	0.19203476565716926
1106	12-13	0.18175109845181225
1106	14-15	0.23730252641501437
1106	16-17	0.27208750087092426
1106	18-19	0.2392213659633171
1106	20-21	0.23231854264986396
1106	22-23	0.2100178692656982
1106	24-25	0.2057513952853327
1106	26-27	0.26756654784583844
1106	28-29	0.23443225579392646
1106	30-31	0.25491632134900044
1106	32-33	0.2019088123758479
1106	34-35	0.22315058437119717
1106	36-37	0.28139789393860326
1106	38-39	0.29303091433554584
1106	40-41	0.26687685874801303
1106	42-43	0.2660666886062444
1106	44-45	0.25434612880279417
1106	46-47	0.286356883527354
1106	48-49	0.29613693512136763
1106	50-51	0.35903441741806574
1106	52-53	0.3272040458236489
1106	54-55	0.3242079533723299
1106	56-57	0.3261273331366823
1106	58-59	0.32284530285650703
1106	60-61	0.3039670435642492
1106	62-63	0.30415458736385403
1106	64-65	0.26145535636554484
1106	66-67	0.2786921510150151
1106	68-69	0.30119767251574814
1106	70-71	0.3201369563395531
1106	72-73	0.38590750993297007
1106	74-75	0.3980412652968184
1106	76-77	0.3699856790114353
1106	78-79	0.40450480081270257
1106	80-81	0.4011307964271609
1106	82-83	0.4189388848412037
1106	84-85	0.3438798142709132
1106	86-87	0.39463658218448217
1106	88-89	0.3930100856462957
1106	90-91	0.3410818079557174
1106	92-93	0.43907367811899434
1106	94-95	0.45991992170821305
1106	96-97	0.4654383378460416
1106	98-99	0.4483882369176584
1106	100	0.4920971133590193
1107	1	0.1074490712057532
1107	2	0.08136576981242172
1107	3	0.24308482727853686
1107	4	0.14772403524987965
1107	5	0.1790587300767399
1107	6	0.15101722957444252
1107	7	0.10504776273688066
1107	8	0.1418180774328448
1107	9	0.13161155591748752
1107	10-11	0.13985234296324478
1107	12-13	0.14189178896543808
1107	14-15	0.182526173558621
1107	16-17	0.1353924995606519
1107	18-19	0.15894629062265153
1107	20-21	0.1906244421519574
1107	22-23	0.1381813585214644
1107	24-25	0.13274137170042621
1107	26-27	0.24449715253661708
1107	28-29	0.2613792388914149
1107	30-31	0.18486219071482424
1107	32-33	0.18599309565677657
1107	34-35	0.14992600365840758
1107	36-37	0.18791363358744206
1107	38-39	0.18108655178313882
1107	40-41	0.16074109813480675
1107	42-43	0.14721800870582769
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2216	78-79	-1.0291052384972375
2216	80-81	-1.2060957042000808
2216	82-83	-1.0332833778595543
2216	84-85	-1.1385267718329146
2216	86-87	-0.9668142494205902
2216	88-89	-1.3484133842299357
2216	90-91	-1.0930701157249985
2216	92-93	-1.1451678615154783
2216	94-95	-1.059743678797517
2216	96-97	-1.1073980053302641
2216	98-99	-1.2631221861287791
2216	100	-1.2537133232037263
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	226.0
3	38.0
4	36.0
5	92.0
6	241.0
7	610.0
8	1595.0
9	2646.0
10	3837.0
11	5441.0
12	7476.0
13	10648.0
14	12885.0
15	16360.0
16	20336.0
17	27252.0
18	27302.0
19	15728.0
20	12195.0
21	12064.0
22	12896.0
23	14628.0
24	17356.0
25	21410.0
26	27210.0
27	34850.0
28	45580.0
29	60077.0
30	78217.0
31	100865.0
32	129782.0
33	169354.0
34	232328.0
35	337962.0
36	548916.0
37	996637.0
38	1378590.0
39	548466.0
40	2036.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.63601966571369	20.428714018116313	14.613626793024427	23.321639523145574
2	15.59247678635993	20.6095738937142	39.55846254120249	24.239486778723386
3	18.058323105334072	25.101111271444342	30.859062763975608	25.981502859245975
4	12.674279706033401	17.177168340100437	37.939380231633876	32.20917172223229
5	12.817642204318943	37.675754048098895	35.51099192406906	13.995611823513102
6	30.945176572828487	38.02014848298639	17.04844666821235	13.986228275972767
7	26.61498351900462	32.89207015245529	22.252667521657145	18.24027880688294
8	25.085936839741503	36.20862937403365	20.12155292495339	18.583880861271464
9	24.74948157419853	16.414682272674945	21.47040797962291	37.36542817350362
10-11	23.94916427653051	26.93091317523035	28.579063380087582	20.540859168151552
12-13	24.528149629608002	24.39832589629673	29.02017650999418	22.053347964101093
14-15	21.75080943304777	26.3964499757659	26.604631159871374	25.24810943131495
16-17	20.916375457460656	29.756934680470415	27.303927156911474	22.022762705157454
18-19	20.754249122774663	28.08845791654131	30.192217914052538	20.965075046631494
20-21	21.77591345242812	27.298519607674248	30.08101674751452	20.844550192383107
22-23	21.413133885996587	27.118655059981744	30.187794578538874	21.280416475482795
24-25	21.37808378036964	27.247194711646127	30.072972862874614	21.301748645109615
26-27	21.1514026336171	27.239130353274604	30.13043038998801	21.479036623120287
28-29	21.176333846221198	27.224495718818552	30.277847135637494	21.32132329932276
30-31	21.411857074992177	27.55446308273249	29.89357070938809	21.140109132887247
32-33	21.14894079278305	27.344693992617188	30.206476803963874	21.29988841063589
34-35	21.266526352483144	27.126028416155517	30.18877560843628	21.418669622925055
36-37	21.500642509732902	27.42221001257254	29.756727909364226	21.320419568330333
38-39	21.438514516930717	27.543960183638426	29.726189046649854	21.291336252780997
40-41	21.357395642867015	27.31251020943374	29.86088620519553	21.46920794250371
42-43	21.347579645298044	27.373804060178642	29.688785842727484	21.58983045179583
44-45	21.479441354980278	27.460974558348717	29.514883304186153	21.544700782484856
46-47	21.57097582416469	27.63945249067091	29.15863634406042	21.63093534110399
48-49	21.605666569689113	27.63795411613979	28.929995076134475	21.826384238036624
50-51	21.64383172962678	27.654268193510827	28.760063172070932	21.94183690479146
52-53	21.61631085473398	27.745649568946956	28.74959402732494	21.888445548994127
54-55	21.66923026333723	27.98510219139128	28.588661245197628	21.757006300073865
56-57	21.561399693603967	27.77030714793691	28.627002047166506	22.04129111129261
58-59	21.46671076735811	27.453805235052027	28.9174881332457	22.161995864344167
60-61	21.64598432154612	27.724516432381602	28.669449308494254	21.960049937578027
62-63	21.48617179963195	27.78978824998176	28.67014786831289	22.053892082073396
64-65	21.45340855337479	27.58538154322957	28.81837100077703	22.142838902618607
66-67	21.442522465628436	27.627701788352997	28.626588513836282	22.303187232182285
68-69	21.746913915531234	27.7291286488029	28.455535009132394	22.068422426533477
70-71	21.704227228946063	27.59305188287529	28.521896155208324	22.18082473297032
72-73	21.545122230138762	27.689426339822475	28.565986865525716	22.199464564513043
74-75	21.63646285277965	27.666021330055475	28.416327742499035	22.281188074665835
76-77	21.665889426842885	27.65197369261067	28.304865278092763	22.377271602453686
78-79	21.65952471336085	27.732336763484167	28.33229732279609	22.275841200358897
80-81	21.579799988528617	27.618398963765223	28.37854024418643	22.423260803519728
82-83	21.47249105180292	27.638060033102324	28.4496679177912	22.439780997303558
84-85	21.54110581938788	27.540195202062094	28.32699856739404	22.591700411155983
86-87	21.591896539371653	27.617790475831455	28.278743221013723	22.511569763783164
88-89	21.45001999366445	27.500489961315854	28.337487786434828	22.712002258584864
90-91	21.365235559776597	27.41459132758669	28.369635426578732	22.850537686057983
92-93	21.463097622768853	27.515624371394754	28.218567812148205	22.802710193688192
94-95	21.33263450474273	27.342063826479723	28.376757787996727	22.948543880780814
96-97	21.30615269308768	27.339186323799623	28.40267393487072	22.951987048241975
98-99	21.273620091712335	27.38052350677121	28.340360572511493	23.005495829004964
100	21.35690379129995	27.26855591896858	28.282198567272644	23.09234172245883
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	767.0
1	564.0
2	696.5
3	1041.5
4	688.5
5	426.0
6	1021.0
7	2586.5
8	4344.5
9	4951.0
10	3782.0
11	2459.0
12	2302.5
13	2599.5
14	2999.5
15	3328.5
16	3870.0
17	4542.5
18	5248.5
19	6091.5
20	7333.0
21	9065.0
22	11359.0
23	14823.0
24	19750.0
25	26072.0
26	34629.5
27	45236.0
28	58295.5
29	73141.0
30	87233.0
31	99210.0
32	109094.0
33	119387.0
34	129490.0
35	137772.0
36	146268.5
37	155588.0
38	163393.5
39	167387.0
40	171293.0
41	174834.5
42	174747.0
43	174639.0
44	173081.5
45	170126.0
46	167858.5
47	162486.5
48	156826.5
49	153204.0
50	149248.0
51	144066.5
52	136335.0
53	129836.5
54	124068.5
55	116990.0
56	108798.0
57	99315.0
58	91376.0
59	84382.5
60	75065.5
61	63754.5
62	54180.0
63	45866.5
64	37321.0
65	30092.0
66	23804.0
67	18179.0
68	14077.0
69	11003.5
70	8207.0
71	6076.5
72	4543.0
73	3227.5
74	2300.0
75	1590.0
76	1032.0
77	696.5
78	474.5
79	303.0
80	178.5
81	94.5
82	56.0
83	33.0
84	19.0
85	4.5
86	3.5
87	4.5
88	2.5
89	2.5
90	2.0
91	0.5
92	1.0
93	1.5
94	0.5
95	0.5
96	0.5
97	0.0
98	0.0
99	0.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.039094736944506145
2	0.0
3	0.0
4	4.053368267963312E-5
5	0.0
6	0.0
7	0.0
8	4.256036681361478E-4
9	0.0
10-11	0.0
12-13	0.042479299448255514
14-15	9.120078602917452E-5
16-17	1.013342066990828E-5
18-19	5.06671033495414E-5
20-21	6.0800524019449686E-5
22-23	0.0
24-25	1.013342066990828E-4
26-27	7.093394468935796E-5
28-29	3.2426946143706496E-4
30-31	0.0
32-33	1.2160104803889937E-4
34-35	6.0800524019449686E-5
36-37	3.0400262009724843E-5
38-39	3.141360407671567E-4
40-41	1.2160104803889937E-4
42-43	5.168044541653224E-4
44-45	3.0400262009724845E-4
46-47	1.1146762736899108E-4
48-49	7.397397089033044E-4
50-51	5.168044541653224E-4
52-53	6.181386608644051E-4
54-55	3.9520340612642297E-4
56-57	6.282720815343134E-4
58-59	1.013342066990828E-4
60-61	1.6213473071853248E-4
62-63	9.72808384311195E-4
64-65	4.458705094759643E-4
66-67	0.001520013100486242
68-69	0.0017024146725445912
70-71	5.168044541653224E-4
72-73	0.001084276011680186
74-75	0.0010234754876607363
76-77	8.30940494932479E-4
78-79	0.003587230917147531
80-81	0.003060293042312301
82-83	0.0014896128384765171
84-85	0.0030906933043220253
86-87	0.0015909470451756001
88-89	0.002128018340680739
90-91	0.003141360407671567
92-93	0.0026245559535062447
94-95	0.003992567743943863
96-97	0.003364295662409549
98-99	0.003110960145661842
100	0.001661880989864958
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4934168.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.89170574024744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.15940308173664	47.75527070627985
2	13.78598177031349	17.06475854137316
3	4.106977313828251	7.625634941679898
4	1.7067166917778116	4.2252642907792355
5	0.9192090906456846	2.8445709276001563
6	0.5429766163541137	2.0163449377934413
7	0.3511099576063567	1.5211555925050368
8	0.24618344085193056	1.2189370463463436
9	0.18214709951602928	1.0146055216217085
>10	0.9372119312511238	10.30579419301715
>50	0.04845348838470638	2.0371136439977704
>100	0.012876005455088017	1.3049341818900124
>500	3.9313707052348484E-4	0.1850847772637585
>1k	2.6209106058412186E-4	0.2636157970560854
>5k	3.2761382571877484E-5	0.14061807188240336
>10k+	6.552276514375497E-5	0.4762968289138651
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12117	0.2455733165145573	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11373	0.23049478655773376	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6935	0.14055054469162787	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.053368267963312E-5	0.0
5	0.0	2.026684133981656E-5	0.0	4.053368267963312E-5	0.0
6	0.0	2.026684133981656E-5	0.0	4.053368267963312E-5	0.0
7	0.0	2.026684133981656E-5	0.0	4.053368267963312E-5	0.0
8	2.026684133981656E-5	2.026684133981656E-5	0.0	4.053368267963312E-5	0.0
9	4.053368267963312E-5	2.026684133981656E-5	0.0	4.053368267963312E-5	0.0
10-11	4.053368267963312E-5	2.026684133981656E-5	0.0	6.080052401944968E-5	0.0
12-13	5.06671033495414E-5	5.06671033495414E-5	0.0	8.106736535926624E-5	0.0
14-15	6.080052401944968E-5	6.080052401944968E-5	0.0	8.106736535926624E-5	0.0
16-17	7.093394468935796E-5	6.080052401944968E-5	0.0	8.106736535926624E-5	0.0
18-19	9.120078602917452E-5	6.080052401944968E-5	0.0	1.013342066990828E-4	0.0
20-21	1.013342066990828E-4	6.080052401944968E-5	0.0	1.520013100486242E-4	0.0
22-23	1.013342066990828E-4	6.080052401944968E-5	0.0	3.8506998545651466E-4	0.0
24-25	1.013342066990828E-4	6.080052401944968E-5	0.0	8.106736535926624E-4	0.0
26-27	1.013342066990828E-4	6.080052401944968E-5	0.0	0.0016720144105348662	0.0
28-29	1.013342066990828E-4	6.080052401944968E-5	0.0	0.005168044541653222	0.0
30-31	1.013342066990828E-4	6.080052401944968E-5	0.0	0.01526093152888187	0.0
32-33	1.1146762736899109E-4	6.080052401944968E-5	0.0	0.03884140142775844	0.0
34-35	1.2160104803889936E-4	6.080052401944968E-5	0.0	0.07602092186565193	0.0
36-37	1.2160104803889936E-4	6.080052401944968E-5	0.0	0.13457182649638197	0.0
38-39	1.2160104803889936E-4	6.080052401944968E-5	0.0	0.21304503616415169	0.0
40-41	1.2160104803889936E-4	7.093394468935796E-5	0.0	0.2932105270837961	0.0
42-43	1.2160104803889936E-4	8.106736535926624E-5	0.0	0.37194720568898343	0.0
44-45	1.2160104803889936E-4	8.106736535926624E-5	0.0	0.46770803101961667	0.0
46-47	1.2160104803889936E-4	8.106736535926624E-5	0.0	0.5851543765838536	0.0
48-49	1.2160104803889936E-4	8.106736535926624E-5	0.0	0.7047996744334607	0.0
50-51	1.2160104803889936E-4	8.106736535926624E-5	0.0	0.8220838852669792	0.0
52-53	1.2160104803889936E-4	8.106736535926624E-5	0.0	0.9373312785458461	0.0
54-55	1.2160104803889936E-4	8.106736535926624E-5	0.0	1.0628134267013203	0.0
56-57	1.418678893787159E-4	8.106736535926624E-5	0.0	1.2105992337512625	0.0
58-59	1.418678893787159E-4	8.106736535926624E-5	0.0	1.369531803538104	0.0
60-61	1.418678893787159E-4	8.106736535926624E-5	0.0	1.5345140254648808	0.0
62-63	1.418678893787159E-4	8.106736535926624E-5	0.0	1.6956354141164225	0.0
64-65	1.418678893787159E-4	8.106736535926624E-5	0.0	1.8638704640782398	0.0
66-67	1.418678893787159E-4	8.106736535926624E-5	0.0	2.056192654972429	0.0
68-69	1.418678893787159E-4	9.120078602917452E-5	0.0	2.2658125949501517	0.0
70-71	1.418678893787159E-4	1.013342066990828E-4	0.0	2.4845222132687823	0.0
72-73	1.418678893787159E-4	1.013342066990828E-4	0.0	2.707356133800065	0.0
74-75	1.418678893787159E-4	1.013342066990828E-4	0.0	2.9330780792222724	0.0
76-77	1.418678893787159E-4	1.013342066990828E-4	0.0	3.1746385611515455	0.0
78-79	1.418678893787159E-4	1.013342066990828E-4	0.0	3.444116617026417	0.0
80-81	1.6213473071853248E-4	1.013342066990828E-4	0.0	3.729017739160888	0.0
82-83	1.7226815138844076E-4	1.013342066990828E-4	0.0	4.019887446069935	0.0
84-85	1.8240157205834905E-4	1.013342066990828E-4	0.0	4.307940062032747	0.0
86-87	1.8240157205834905E-4	1.013342066990828E-4	0.0	4.617556597181126	0.0
88	1.8240157205834905E-4	1.013342066990828E-4	0.0	4.864426991541431	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7910	0.0	55.92912	1
GTATCAA	13910	0.0	40.220238	1
ATCAACG	17180	0.0	32.27938	3
TCAACGC	17250	0.0	32.230125	4
CAACGCA	17795	0.0	31.243027	5
TATCAAC	17940	0.0	30.938112	2
AACGCAG	18685	0.0	29.852102	6
GTGGTAT	3655	0.0	29.198685	1
ACGCAGA	21710	0.0	25.56263	7
CGCAGAG	21960	0.0	25.185947	8
TGGTATC	3915	0.0	24.968813	2
GTACATG	12775	0.0	23.037651	1
GCAGAGT	24295	0.0	22.416643	9
GAGTACT	17410	0.0	20.846779	12-13
TACATGG	13150	0.0	20.800013	2
CAGAGTA	23420	0.0	20.452814	10-11
AGAGTAC	22225	0.0	20.124157	10-11
ACATGGG	12970	0.0	20.074102	3
AGTACTT	18070	0.0	18.862545	12-13
GTACTTT	19250	0.0	18.566517	14-15
>>END_MODULE