FastQCFastQC Report
Wed 25 May 2016
SRR2049357_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049357_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6068403
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55300.040.3844071
GTATCAA88250.033.4572451
ATCAACG90900.031.1347663
TCAACGC91000.030.9972294
CAACGCA95250.029.6141515
AACGCAG103050.027.5968516
TATCAAC106850.026.9711192
GTACATG145500.026.7877831
TACATGG144450.025.8413962
ACATGGG151850.023.4365713
ACGCAGA121050.023.1820017
CGCAGAG122850.022.7658148
CATGGGG101850.020.078964
GTGGTAT34550.019.459571
TCTGTCG17800.018.7490718
GCAGAGT143650.018.4880629
GAGTACA112550.018.21321
TACCTGG43450.017.7446352
TGGTATC34900.016.8382742
AGAGTAC124050.016.76737410-11
Produced by FastQC (version 0.11.5)