##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049357_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6068403
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.946459884091414	31.0	31.0	34.0	28.0	34.0
2	31.02644155307418	31.0	31.0	34.0	28.0	34.0
3	31.207153018677236	31.0	31.0	34.0	28.0	34.0
4	34.80036510429515	37.0	35.0	37.0	32.0	37.0
5	34.84681241506208	37.0	35.0	37.0	32.0	37.0
6	34.80322928454159	37.0	35.0	37.0	32.0	37.0
7	34.77078598768078	37.0	35.0	37.0	32.0	37.0
8	34.75649293562079	37.0	35.0	37.0	32.0	37.0
9	36.36363405660435	39.0	37.0	39.0	32.0	39.0
10-11	36.32390284560864	39.0	37.0	39.0	32.0	39.0
12-13	36.263520319926016	39.0	37.0	39.0	32.0	39.0
14-15	37.47971673931346	40.0	38.0	41.0	32.0	41.0
16-17	37.43963543620949	40.0	38.0	41.0	32.0	41.0
18-19	37.45970059997663	40.0	38.0	41.0	32.0	41.0
20-21	37.541144762468804	40.0	38.0	41.0	32.0	41.0
22-23	37.48644635170077	40.0	38.0	41.0	32.0	41.0
24-25	37.49236940921689	40.0	38.0	41.0	32.0	41.0
26-27	37.48603306339411	40.0	38.0	41.0	32.0	41.0
28-29	37.42048954230627	40.0	38.0	41.0	32.0	41.0
30-31	37.35324301962147	40.0	38.0	41.0	32.0	41.0
32-33	37.300841094436215	40.0	37.5	41.0	31.5	41.0
34-35	37.19687288401907	40.0	37.0	41.0	31.0	41.0
36-37	37.110845884823405	40.0	37.0	41.0	31.0	41.0
38-39	37.005089312624754	40.0	37.0	41.0	30.5	41.0
40-41	36.89993553493399	40.0	37.0	41.0	30.0	41.0
42-43	36.75317567076544	40.0	37.0	41.0	30.0	41.0
44-45	36.62295574305134	40.0	36.5	41.0	30.0	41.0
46-47	36.504835209527116	40.0	36.0	41.0	30.0	41.0
48-49	36.37516386106856	40.0	36.0	41.0	30.0	41.0
50-51	36.23714624753828	39.5	36.0	41.0	29.5	41.0
52-53	36.090513517312544	39.0	35.0	41.0	29.0	41.0
54-55	35.944470810524614	39.0	35.0	41.0	29.0	41.0
56-57	35.82115920778498	39.0	35.0	41.0	29.0	41.0
58-59	35.61239530400338	39.0	35.0	41.0	28.0	41.0
60-61	35.38677399968327	39.0	35.0	40.0	28.0	41.0
62-63	35.16438130427396	38.0	34.5	40.0	28.0	41.0
64-65	34.907287881177304	38.0	34.0	40.0	28.0	41.0
66-67	34.64081217084627	37.0	34.0	40.0	27.5	41.0
68-69	34.341779377539694	37.0	34.0	40.0	26.5	41.0
70-71	34.03998523169935	36.0	34.0	39.0	26.0	41.0
72-73	33.68370154388231	36.0	34.0	39.0	26.0	41.0
74-75	33.32769560624105	35.5	33.0	39.0	26.0	40.0
76-77	32.98020286391659	35.0	33.0	37.5	26.0	40.0
78-79	32.647937933588125	35.0	33.0	37.0	25.0	39.0
80-81	32.272598078934436	35.0	33.0	37.0	24.5	39.0
82-83	31.943022159207292	35.0	33.0	36.0	24.0	38.5
84-85	31.632190709812782	35.0	32.5	36.0	23.0	37.0
86-87	31.29093519332846	35.0	32.0	35.5	21.0	37.0
88-89	31.017479145666496	35.0	32.0	35.0	19.5	36.5
90-91	30.78915746037302	35.0	32.0	35.0	18.5	36.0
92-93	30.5259942360453	35.0	32.0	35.0	13.0	36.0
94-95	30.300968722743036	35.0	32.0	35.0	4.5	36.0
96-97	30.021885906390857	35.0	31.5	35.0	2.0	35.5
98-99	29.79242034518802	34.5	31.0	35.0	2.0	35.0
100	29.666791905547473	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.42066721719106326
1101	2	0.3864284238144684
1101	3	0.41900289153494796
1101	4	0.40562510454107326
1101	5	0.4298798431967512
1101	6	0.4693710693587647
1101	7	0.512549069965111
1101	8	0.5310438591604054
1101	9	0.6399613232698371
1101	10-11	0.6275866935517342
1101	12-13	0.6986740689453868
1101	14-15	0.7458316644740464
1101	16-17	0.7595211143596288
1101	18-19	0.7380040781178323
1101	20-21	0.7107150872709411
1101	22-23	0.7154760832147815
1101	24-25	0.7303330339856444
1101	26-27	0.6798283808712995
1101	28-29	0.7204580340525553
1101	30-31	0.7267802523848559
1101	32-33	0.7067547990871645
1101	34-35	0.7746127555576194
1101	36-37	0.7928803989002091
1101	38-39	0.8087714652042166
1101	40-41	0.8188446792167525
1101	42-43	0.7502612019670565
1101	44-45	0.6961650038865486
1101	46-47	0.6507477231236223
1101	48-49	0.5970675448073521
1101	50-51	0.6295266886488236
1101	52-53	0.6493936209181612
1101	54-55	0.671662488014455
1101	56-57	0.6459114977118006
1101	58-59	0.6795017195928921
1101	60-61	0.6503088559892234
1101	62-63	0.7087742915368622
1101	64-65	0.6527540220908108
1101	66-67	0.6997889964393309
1101	68-69	0.6736557982358633
1101	70-71	0.6389255466965054
1101	72-73	0.7774519088635756
1101	74-75	0.7144004997889297
1101	76-77	0.7074705364533855
1101	78-79	0.6435668323555177
1101	80-81	0.682023034691035
1101	82-83	0.670118383789287
1101	84-85	0.5938995562129996
1101	86-87	0.6273453781895277
1101	88-89	0.6519682720346722
1101	90-91	0.7321824056248687
1101	92-93	0.7501990314571891
1101	94-95	0.6663154106727198
1101	96-97	0.6702798006460498
1101	98-99	0.7564038932071568
1101	100	0.7873031343492016
1102	1	0.13135030159003236
1102	2	0.2105025741061617
1102	3	0.2275064683515602
1102	4	0.20946056183077388
1102	5	0.1659258454073722
1102	6	0.2809947287099135
1102	7	0.3205078241911892
1102	8	0.32438180670835237
1102	9	0.39328976571859897
1102	10-11	0.3499691442621753
1102	12-13	0.42321191579461725
1102	14-15	0.46129802369408424
1102	16-17	0.45726001772224834
1102	18-19	0.476562575063717
1102	20-21	0.3703125221974801
1102	22-23	0.3976904009078055
1102	24-25	0.4834854708991756
1102	26-27	0.3968789328164206
1102	28-29	0.4806428324333254
1102	30-31	0.419667770957183
1102	32-33	0.4457678007458057
1102	34-35	0.43272940601278975
1102	36-37	0.4314989915327416
1102	38-39	0.4247158689484465
1102	40-41	0.3999473610403612
1102	42-43	0.37115482534196786
1102	44-45	0.2849904073102181
1102	46-47	0.31725021704031064
1102	48-49	0.37110496201787413
1102	50-51	0.39903752244974555
1102	52-53	0.3648166504221493
1102	54-55	0.3233048900401414
1102	56-57	0.26182080324277734
1102	58-59	0.34611245205486796
1102	60-61	0.30086981575501426
1102	62-63	0.3180842183633743
1102	64-65	0.3137955427054706
1102	66-67	0.3505292200435335
1102	68-69	0.40334436504434024
1102	70-71	0.3747922564871047
1102	72-73	0.34076899984135167
1102	74-75	0.36420639066388816
1102	76-77	0.3345016026021881
1102	78-79	0.3633577316060439
1102	80-81	0.3074192900889585
1102	82-83	0.3278052526216655
1102	84-85	0.36536848732034954
1102	86-87	0.41612198709390924
1102	88-89	0.41087147116249767
1102	90-91	0.4359760651726603
1102	92-93	0.383396950853399
1102	94-95	0.39347256172217016
1102	96-97	0.4085932103128549
1102	98-99	0.4518358321696496
1102	100	0.4072533360438868
1103	1	0.23572728726527004
1103	2	0.26807290769200165
1103	3	0.29670507245972644
1103	4	0.28721753070546185
1103	5	0.28838614058729206
1103	6	0.3315520041979312
1103	7	0.3562344274465161
1103	8	0.34146819407072826
1103	9	0.456580358386411
1103	10-11	0.4132257309015017
1103	12-13	0.42338726531578885
1103	14-15	0.5110797961805602
1103	16-17	0.4911500647520697
1103	18-19	0.44535904499370105
1103	20-21	0.4401238726010277
1103	22-23	0.44534454401993173
1103	24-25	0.5142259164807399
1103	26-27	0.4751767685240509
1103	28-29	0.48955608932392636
1103	30-31	0.4998675966769639
1103	32-33	0.49893135470743033
1103	34-35	0.4716844379388405
1103	36-37	0.4805786331629349
1103	38-39	0.5158617005855533
1103	40-41	0.48133226835615517
1103	42-43	0.5012744831387366
1103	44-45	0.3743979555303838
1103	46-47	0.40361622047814905
1103	48-49	0.43121326348378375
1103	50-51	0.3722430517377049
1103	52-53	0.3813832449189576
1103	54-55	0.3714689607414172
1103	56-57	0.31058521740222744
1103	58-59	0.3485555802968463
1103	60-61	0.3772274216307352
1103	62-63	0.41355038971988733
1103	64-65	0.3987071845675487
1103	66-67	0.39137039240421245
1103	68-69	0.46147254133767035
1103	70-71	0.4150617041901228
1103	72-73	0.4673363575061771
1103	74-75	0.4394845968868708
1103	76-77	0.4864838256726216
1103	78-79	0.3636931238646355
1103	80-81	0.3526545542699324
1103	82-83	0.2893260201276817
1103	84-85	0.3621793162076621
1103	86-87	0.36608905036911565
1103	88-89	0.3532306422382767
1103	90-91	0.40867326811303784
1103	92-93	0.37398527360116773
1103	94-95	0.4171783867241601
1103	96-97	0.3488465587532197
1103	98-99	0.3468080639269502
1103	100	0.3766410440668757
1104	1	0.14000091454817465
1104	2	0.13409612368231905
1104	3	0.16497658776157564
1104	4	0.22438706578028444
1104	5	0.2284446772666726
1104	6	0.2823672884109456
1104	7	0.27536357175148396
1104	8	0.30245057973117895
1104	9	0.3890955801170932
1104	10-11	0.3843254505120086
1104	12-13	0.3452852050722157
1104	14-15	0.4213176419474607
1104	16-17	0.4591493307964356
1104	18-19	0.3944632727817847
1104	20-21	0.3367203469562412
1104	22-23	0.34326294931105394
1104	24-25	0.4253286222706052
1104	26-27	0.3886839276481595
1104	28-29	0.41204803994120454
1104	30-31	0.36208827149806666
1104	32-33	0.4075909653078824
1104	34-35	0.40159584492899825
1104	36-37	0.3815624184006907
1104	38-39	0.4345935467818762
1104	40-41	0.4077699882154917
1104	42-43	0.3975047762135304
1104	44-45	0.37637423353704946
1104	46-47	0.31593649116774003
1104	48-49	0.31297654333658187
1104	50-51	0.3761558131809082
1104	52-53	0.38579291070077915
1104	54-55	0.34572521544406953
1104	56-57	0.3614989473591379
1104	58-59	0.3969618702557369
1104	60-61	0.3632482399739132
1104	62-63	0.35422964398220813
1104	64-65	0.42367181758855565
1104	66-67	0.45790206319617255
1104	68-69	0.4617844996830627
1104	70-71	0.41079957297245073
1104	72-73	0.4412681812284447
1104	74-75	0.4853543116531185
1104	76-77	0.5027967823986899
1104	78-79	0.38136726859480774
1104	80-81	0.4131515225907094
1104	82-83	0.3420223525487174
1104	84-85	0.36377665535814074
1104	86-87	0.37781529595081764
1104	88-89	0.3480601327696071
1104	90-91	0.3476149146505847
1104	92-93	0.31741204341950535
1104	94-95	0.31800237199241366
1104	96-97	0.27232840011202697
1104	98-99	0.293456782173962
1104	100	0.3085945698113939
1105	1	0.08808195624411042
1105	2	0.09222522869273675
1105	3	0.09269629060856133
1105	4	0.10532873917544094
1105	5	0.07686142742423385
1105	6	0.1257091890683668
1105	7	0.09226913323433905
1105	8	0.17995884853616673
1105	9	0.2387416274408949
1105	10-11	0.2062274411994025
1105	12-13	0.18544195543658049
1105	14-15	0.24521848643088617
1105	16-17	0.21687238148164312
1105	18-19	0.15426541825170403
1105	20-21	0.14810142853094987
1105	22-23	0.20231523767741066
1105	24-25	0.16138352388457378
1105	26-27	0.14183129079243884
1105	28-29	0.1805853736690466
1105	30-31	0.22045711021056036
1105	32-33	0.23861713253246108
1105	34-35	0.22077406634775087
1105	36-37	0.2128890186246153
1105	38-39	0.223079773344395
1105	40-41	0.23681733548183814
1105	42-43	0.266537165709515
1105	44-45	0.15210482733503738
1105	46-47	0.08405497849269494
1105	48-49	0.13371625451923563
1105	50-51	0.1633104419966429
1105	52-53	0.17002248113254836
1105	54-55	0.194698612993065
1105	56-57	0.15952826521540686
1105	58-59	0.15411085579289363
1105	60-61	0.09231804328338455
1105	62-63	0.04739609445244497
1105	64-65	0.0686189642977908
1105	66-67	0.13690595120235116
1105	68-69	0.07282907367965663
1105	70-71	0.09805674347074955
1105	72-73	0.06157326003997099
1105	74-75	0.13731260562716585
1105	76-77	0.18138774591493245
1105	78-79	0.14381707463444826
1105	80-81	0.17826175890747464
1105	82-83	0.12838318403947824
1105	84-85	0.19359532402162571
1105	86-87	0.24999417863534745
1105	88-89	0.21869190481574918
1105	90-91	0.23336991020983078
1105	92-93	0.1951137158124645
1105	94-95	0.14936771010244598
1105	96-97	0.14411633090292497
1105	98-99	0.16802839111384316
1105	100	0.1717347988215252
1106	1	0.12415027888422614
1106	2	0.08538751403304445
1106	3	0.19160319672909054
1106	4	0.21656488769840365
1106	5	0.1513411995340448
1106	6	0.21506336405704474
1106	7	0.219732196042699
1106	8	0.2575714155190312
1106	9	0.3231271675527907
1106	10-11	0.310480496289955
1106	12-13	0.2913362819542371
1106	14-15	0.42945340834082657
1106	16-17	0.42401022334564686
1106	18-19	0.3787031530242402
1106	20-21	0.3436371572140189
1106	22-23	0.3814654510154867
1106	24-25	0.3669451074189638
1106	26-27	0.3821710312682143
1106	28-29	0.4184916279809272
1106	30-31	0.4156880986742095
1106	32-33	0.4598509581331669
1106	34-35	0.5337303167152712
1106	36-37	0.4286214797287542
1106	38-39	0.4388574955857507
1106	40-41	0.4623682226628887
1106	42-43	0.43842100520961225
1106	44-45	0.40302787693245534
1106	46-47	0.39026434425066725
1106	48-49	0.4225137332107707
1106	50-51	0.465997338629478
1106	52-53	0.4931267793522167
1106	54-55	0.4918334164467595
1106	56-57	0.4269259161454926
1106	58-59	0.45559959960154117
1106	60-61	0.46983028382602754
1106	62-63	0.417933273905831
1106	64-65	0.4670362740588345
1106	66-67	0.5107582072153676
1106	68-69	0.5460018019109327
1106	70-71	0.4671090136390461
1106	72-73	0.46865767927850754
1106	74-75	0.4677820133469268
1106	76-77	0.5386609258209774
1106	78-79	0.4840667744143019
1106	80-81	0.5186846760768447
1106	82-83	0.48998960487372045
1106	84-85	0.47195416025568093
1106	86-87	0.4918495275168837
1106	88-89	0.4676314363709473
1106	90-91	0.44589485332080514
1106	92-93	0.42201202101341195
1106	94-95	0.4820468751674767
1106	96-97	0.49465887193027314
1106	98-99	0.51245315319958
1106	100	0.5020829972158367
1107	1	0.16778278383873513
1107	2	0.1250938113807365
1107	3	0.1384586207697005
1107	4	0.16941525479599306
1107	5	0.15503485090842162
1107	6	0.204595107752354
1107	7	0.125536464494715
1107	8	0.2691562158771674
1107	9	0.2803420672123522
1107	10-11	0.25644163462039415
1107	12-13	0.2617403493829471
1107	14-15	0.2504256687460398
1107	16-17	0.3523807768228693
1107	18-19	0.2579352516593758
1107	20-21	0.236092241815399
1107	22-23	0.2720659466328641
1107	24-25	0.2925105879681027
1107	26-27	0.31049186774183113
1107	28-29	0.37865895550463335
1107	30-31	0.33184444575308447
1107	32-33	0.3880710608086204
1107	34-35	0.3712097862219039
1107	36-37	0.34781866408852835
1107	38-39	0.38726993919816266
1107	40-41	0.4371777542520334
1107	42-43	0.39031932270586367
1107	44-45	0.3136516933989455
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2208	94-95	0.5330784365505181
2208	96-97	0.5261230934362153
2208	98-99	0.6272217978988515
2208	100	0.5710974498623607
2209	1	0.12033885999259297
2209	2	0.0905708001888712
2209	3	0.13455435068534527
2209	4	0.09733666442313904
2209	5	0.14014682887122376
2209	6	0.13736876545829801
2209	7	0.16967900116646462
2209	8	0.10117691475223012
2209	9	0.09839288569881433
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2209	14-15	0.18737696531500347
2209	16-17	0.24394293689522328
2209	18-19	0.21085572216057358
2209	20-21	0.20854603771753943
2209	22-23	0.17552709081358842
2209	24-25	0.17875034160975645
2209	26-27	0.195244555888749
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2209	58-59	0.3469883221517165
2209	60-61	0.3011714434584576
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2209	84-85	0.28347463326490896
2209	86-87	0.22273122006224355
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2210	2	-0.48717282049398847
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2210	4	-0.444432050200561
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2210	8	-0.38247579021505373
2210	9	-0.4900381909874483
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2210	12-13	-0.6064620598348043
2210	14-15	-0.5299938656019165
2210	16-17	-0.6916237927805824
2210	18-19	-0.4913166377655429
2210	20-21	-0.5794769110258571
2210	22-23	-0.6894089296244204
2210	24-25	-0.6699027148669643
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2210	28-29	-0.5785290201413247
2210	30-31	-0.5597793980752783
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2210	70-71	-1.072810895409873
2210	72-73	-1.1154884347459912
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2210	80-81	-1.2765814373211555
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2210	88-89	-1.631978358227375
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2210	92-93	-1.2016652621176362
2210	94-95	-1.0697788938389436
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2210	98-99	-1.1831778274431421
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2211	2	0.0349549896385426
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2211	4	0.06066519834341477
2211	5	0.07879823670276664
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2211	8	0.06689405370476464
2211	9	0.10697178082867964
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2211	12-13	0.08752678011172321
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2211	20-21	0.0883095206472504
2211	22-23	0.09036552389937924
2211	24-25	0.08641403800582026
2211	26-27	0.056710415814976045
2211	28-29	0.1439331186356796
2211	30-31	0.10146739564248719
2211	32-33	0.09261415767811343
2211	34-35	0.17919512305316943
2211	36-37	0.13659102633322817
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2211	40-41	0.1386045924253807
2211	42-43	0.22494801322939395
2211	44-45	0.21864591879527495
2211	46-47	0.14555476745837126
2211	48-49	0.17190206712324851
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2211	54-55	0.06846543679666439
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2211	58-59	0.2481552706877892
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2211	62-63	0.238047830174132
2211	64-65	0.2609961079820877
2211	66-67	0.20135041180948576
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2211	70-71	0.11448922832512665
2211	72-73	0.16485037078796694
2211	74-75	0.28351580507470686
2211	76-77	0.21156932350857005
2211	78-79	0.1766284739218804
2211	80-81	0.17046928108876358
2211	82-83	0.2156971878035776
2211	84-85	0.1952808739003693
2211	86-87	0.19673672779824614
2211	88-89	0.2424003360816407
2211	90-91	0.23980144131719783
2211	92-93	0.2852966341679881
2211	94-95	0.2382318530228602
2211	96-97	0.2733959035993543
2211	98-99	0.221534732552783
2211	100	0.27128192024045816
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2212	2	-0.22620941351392077
2212	3	-0.3578607703739536
2212	4	-0.2909892197200463
2212	5	-0.3109532545172158
2212	6	-0.4022936076332684
2212	7	-0.36675621942782755
2212	8	-0.39953962165319723
2212	9	-0.4682307257110665
2212	10-11	-0.5703078292579704
2212	12-13	-0.6748240757702817
2212	14-15	-0.47748902395337467
2212	16-17	-0.4885478976796449
2212	18-19	-0.41768272615054514
2212	20-21	-0.4157265541757198
2212	22-23	-0.3853807858750997
2212	24-25	-0.44356845617593876
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2212	34-35	-0.5235650777216136
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2212	42-43	-0.577393922841388
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2212	48-49	-0.4002631932223082
2212	50-51	-0.3556489263177198
2212	52-53	-0.2701818540730301
2212	54-55	-0.40350597983135117
2212	56-57	-0.48479354524073415
2212	58-59	-0.37889152214560085
2212	60-61	-0.3310896496603988
2212	62-63	-0.3639563885478907
2212	64-65	-0.4145545764452052
2212	66-67	-0.45670218616044167
2212	68-69	-0.4217390649129129
2212	70-71	-0.3520641942618141
2212	72-73	-0.41480651147438863
2212	74-75	-0.35275952764764185
2212	76-77	-0.313017658143778
2212	78-79	-0.4261060319731058
2212	80-81	-0.45426749620302687
2212	82-83	-0.3409960870794322
2212	84-85	-0.37213962117871446
2212	86-87	-0.4050477029787274
2212	88-89	-0.3902872248778415
2212	90-91	-0.4229786183943425
2212	92-93	-0.5244574442356189
2212	94-95	-0.5081925811870676
2212	96-97	-0.3550422997522311
2212	98-99	-0.35240512944371716
2212	100	-0.38369194731472334
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2213	2	-0.1888749794661848
2213	3	-0.3306242608865233
2213	4	-0.3563236414045434
2213	5	-0.29603101154793876
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2213	7	-0.2576756540106615
2213	8	-0.186990868968536
2213	9	-0.2630938975225732
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2213	14-15	-0.4188471071340061
2213	16-17	-0.4432893775745086
2213	18-19	-0.4064777413708427
2213	20-21	-0.26484033226876136
2213	22-23	-0.27669725420840763
2213	24-25	-0.32765814913881997
2213	26-27	-0.32137042293967966
2213	28-29	-0.36958350706328247
2213	30-31	-0.4352800324182624
2213	32-33	-0.4809787641598575
2213	34-35	-0.40703107920752046
2213	36-37	-0.36652274011103003
2213	38-39	-0.3520910945343019
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2213	46-47	-0.33718957661942994
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2213	52-53	-0.3760283272086866
2213	54-55	-0.3319190203027915
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2213	58-59	-0.2709742068919567
2213	60-61	-0.23415680927249127
2213	62-63	-0.12285225397251764
2213	64-65	-0.2115881337462966
2213	66-67	-0.14470592043740282
2213	68-69	-0.1537565248937014
2213	70-71	-0.20631589198532652
2213	72-73	-0.2504977941176705
2213	74-75	-0.22858985903260276
2213	76-77	-0.14169390998908682
2213	78-79	-0.282764528279003
2213	80-81	-0.33631498532627546
2213	82-83	-0.38865442729947475
2213	84-85	-0.3085821877634345
2213	86-87	-0.2706706372074663
2213	88-89	-0.30104287845068356
2213	90-91	-0.32196249557485856
2213	92-93	-0.28635586653210154
2213	94-95	-0.3887298333572957
2213	96-97	-0.3452317045358022
2213	98-99	-0.4249894496144506
2213	100	-0.3771710741147807
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2214	2	-0.13594481420728854
2214	3	-0.168855594579437
2214	4	-0.14945695630704847
2214	5	-0.10361755908348158
2214	6	-0.02382276233734615
2214	7	-0.10114402865402639
2214	8	-0.13150569861043948
2214	9	-0.15509931209314942
2214	10-11	-0.29708555260666003
2214	12-13	-0.2348920046429086
2214	14-15	-0.2304425165855548
2214	16-17	-0.25956353796162546
2214	18-19	-0.2817028775670778
2214	20-21	-0.23686470373618818
2214	22-23	-0.23324752395516413
2214	24-25	-0.23709419251991903
2214	26-27	-0.2521641429744079
2214	28-29	-0.29172030719919917
2214	30-31	-0.28890576940173673
2214	32-33	-0.16544389878156807
2214	34-35	-0.2158905707508012
2214	36-37	-0.2339937919874231
2214	38-39	-0.2197985821674706
2214	40-41	-0.21718703951489715
2214	42-43	-0.09525481055693064
2214	44-45	-0.14029377394092535
2214	46-47	-0.09664792873926586
2214	48-49	-0.11132727158007327
2214	50-51	-0.06430433653083867
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2214	54-55	-0.09766012628340803
2214	56-57	-0.1656005685139732
2214	58-59	-0.1307545653749429
2214	60-61	-0.1598453081128497
2214	62-63	-0.11510508812722975
2214	64-65	-0.10395766011978935
2214	66-67	-0.15463871582284128
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2214	70-71	-0.24193311163983822
2214	72-73	-0.26139132639948315
2214	74-75	-0.11940816753794081
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2214	78-79	-0.08115398483721492
2214	80-81	-0.035650968482997314
2214	82-83	0.033075883648393756
2214	84-85	0.10479848311565121
2214	86-87	0.14980217069852841
2214	88-89	0.11541396469500853
2214	90-91	0.03474659948589931
2214	92-93	0.10790594790399766
2214	94-95	0.09447660903771293
2214	96-97	0.07471681113567286
2214	98-99	0.1322897581708915
2214	100	0.12774060033397916
2215	1	-0.347115360114028
2215	2	-0.24553227711185954
2215	3	-0.30719780154856124
2215	4	-0.2647452445305234
2215	5	-0.2955565698895626
2215	6	-0.3176849235900008
2215	7	-0.4614509649953078
2215	8	-0.38243107163872736
2215	9	-0.5175982677041873
2215	10-11	-0.5228223673406092
2215	12-13	-0.5254412304333513
2215	14-15	-0.614889850164765
2215	16-17	-0.507581109638906
2215	18-19	-0.5858138876685146
2215	20-21	-0.5046112830965654
2215	22-23	-0.5208623733264233
2215	24-25	-0.5868943560805988
2215	26-27	-0.5617397451008657
2215	28-29	-0.5236956113404219
2215	30-31	-0.598249610022151
2215	32-33	-0.55020905672054
2215	34-35	-0.5608451821490306
2215	36-37	-0.5927213888461296
2215	38-39	-0.346662893843245
2215	40-41	-0.5283325531419365
2215	42-43	-0.4651420208543797
2215	44-45	-0.41506255828902994
2215	46-47	-0.41677921522751404
2215	48-49	-0.3756265061840125
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2215	52-53	-0.37130000479749015
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2215	56-57	-0.38302519978164895
2215	58-59	-0.28750878692657267
2215	60-61	-0.22557399163842007
2215	62-63	-0.27117485702398625
2215	64-65	-0.22397439079596637
2215	66-67	-0.21458646757925237
2215	68-69	-0.2569974502467929
2215	70-71	-0.20259193686878518
2215	72-73	-0.28378006558190094
2215	74-75	-0.24267655179334469
2215	76-77	-0.22833364634654174
2215	78-79	-0.28035606718150774
2215	80-81	-0.3568019167899692
2215	82-83	-0.26893495600122463
2215	84-85	-0.28228649662351657
2215	86-87	-0.23266972256745788
2215	88-89	-0.2309208368858009
2215	90-91	-0.2951593424445811
2215	92-93	-0.30155655790865765
2215	94-95	-0.30877085622107003
2215	96-97	-0.2529237688221109
2215	98-99	-0.4326889881436031
2215	100	-0.4344829954093683
2216	1	-1.139482898168037
2216	2	-1.0739529873623255
2216	3	-1.1210678917516823
2216	4	-1.3599925204807946
2216	5	-1.2957008306060018
2216	6	-2.737613107135701
2216	7	-2.606359094950541
2216	8	-3.5095529715175218
2216	9	-2.8401705887865916
2216	10-11	-2.0801485961002797
2216	12-13	-1.881335057461122
2216	14-15	-2.0989303063016465
2216	16-17	-2.4643118443454455
2216	18-19	-2.0713006481394842
2216	20-21	-1.9441949359329058
2216	22-23	-2.0704983380918236
2216	24-25	-2.2273329411865674
2216	26-27	-2.3080892388694707
2216	28-29	-2.7163985377853095
2216	30-31	-2.3424030250017864
2216	32-33	-2.540050958635625
2216	34-35	-3.5334921700613435
2216	36-37	-2.5233743417151047
2216	38-39	-2.448718005596234
2216	40-41	-2.9478000791232546
2216	42-43	-2.7254129226075676
2216	44-45	-2.4276032200454267
2216	46-47	-2.3578078703898626
2216	48-49	-2.503293014263434
2216	50-51	-2.8089016302770062
2216	52-53	-3.1815462999313127
2216	54-55	-2.578310693883239
2216	56-57	-2.619543568351496
2216	58-59	-3.612627662485025
2216	60-61	-4.378982287930736
2216	62-63	-3.419186014371043
2216	64-65	-3.3985259027854973
2216	66-67	-3.0513903811826566
2216	68-69	-3.458739396620345
2216	70-71	-3.2044656064653907
2216	72-73	-3.6182145333561984
2216	74-75	-4.153449587844378
2216	76-77	-4.839135199484858
2216	78-79	-3.7657506428150427
2216	80-81	-3.5633448601621964
2216	82-83	-3.8295416451907656
2216	84-85	-3.8470698810524446
2216	86-87	-3.510328444372007
2216	88-89	-3.4193524081054107
2216	90-91	-3.607633275620941
2216	92-93	-3.6982677157463044
2216	94-95	-3.5475315182007208
2216	96-97	-3.8546201884616735
2216	98-99	-3.629486525551318
2216	100	-3.2052245171772
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	86491.0
3	14655.0
4	9618.0
5	8346.0
6	7515.0
7	8167.0
8	8957.0
9	10209.0
10	11820.0
11	13273.0
12	15059.0
13	16433.0
14	18545.0
15	19237.0
16	19342.0
17	20306.0
18	19425.0
19	18074.0
20	18086.0
21	19266.0
22	21614.0
23	25549.0
24	30948.0
25	37720.0
26	46989.0
27	55404.0
28	68568.0
29	87414.0
30	106833.0
31	128778.0
32	162480.0
33	215593.0
34	295839.0
35	427434.0
36	696614.0
37	1222094.0
38	1539753.0
39	534605.0
40	1350.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.84637191478841	19.725443982034694	12.59056409411183	23.837620009065063
2	16.345681943135894	20.41881908021362	39.5253933280744	23.71010564857609
3	18.367925293499717	25.10605424147725	30.238861914179317	26.28715855084372
4	12.611116359285749	16.534074694329547	38.64357381898348	32.211235127401224
5	12.664838050042018	37.334246927213485	35.52810743784893	14.472807584895564
6	30.943734542830487	37.66459139491202	17.798152220630904	13.593521841626588
7	26.922015467325235	32.16254045294105	22.889415713628622	18.02602836610509
8	24.826569978780697	35.84486414847545	21.107314354164718	18.22125151857913
9	24.812115480932604	16.596501570966158	21.818624546506964	36.77275840159428
10-11	23.645720943341058	26.586912687973356	29.359004763460362	20.408361605225224
12-13	24.186339701459023	24.36318949182187	29.151125127862464	22.299345678856643
14-15	21.695595182022714	26.749439759539666	26.199387737505603	25.355577320932017
16-17	21.23562504966259	29.887334745152188	26.66486315215431	22.21217705303091
18-19	21.069142833685504	28.784609046347185	28.750425135091156	21.395822984876155
20-21	22.345647984151064	28.399447094057894	28.05820432180946	21.196700599981586
22-23	21.89354097285814	28.435023499743515	28.051185150875714	21.62025037652263
24-25	21.68788300018266	28.386029894209024	28.17260816324108	21.75347894236723
26-27	21.41053211002822	28.197618612125634	28.36296048585568	22.02888879199046
28-29	21.583731372354908	28.257227492087512	28.357204684198106	21.801836451359478
30-31	21.93796283474121	28.88411903961173	27.75413460206928	21.423783523577782
32-33	21.334532608932186	28.50446859847633	28.41153078430118	21.7494680082903
34-35	21.459511366821083	27.951862048500637	28.5753556768163	22.01327090786198
36-37	22.053919080907637	28.52849657311161	27.841775356587867	21.57580898939289
38-39	21.83629653997244	28.556929810252512	28.050610650069558	21.556162999705492
40-41	21.536006984547544	28.25075396120187	28.353697237140622	21.859541817109964
42-43	21.43925749776417	28.232427598201443	28.302465803106674	22.025849100927715
44-45	21.649111154577113	28.40464299341078	28.231696656972538	21.714549195039567
46-47	21.505573721631173	28.80724261117049	27.94482681993594	21.742356847262396
48-49	21.42897590123604	28.384409124842087	28.271676058066635	21.914938915855238
50-51	21.513757959095393	28.133720715540477	28.24995845708287	22.102562868281257
52-53	21.45755381759872	28.227559879254844	28.244210063338286	22.07067623980815
54-55	21.604445139754247	28.72253913219512	28.025997516337263	21.647018211713373
56-57	21.344141511902038	28.43008090638094	28.05537847994782	22.170399101769213
58-59	21.163583131803186	27.931295117612827	28.472887807467412	22.432233943116568
60-61	21.608845977361657	28.35922671880539	27.94950054190282	22.082426761930122
62-63	21.484735384957514	28.418270737010875	27.947169390174537	22.149824487857074
64-65	21.46433692223038	28.08392988018071	28.22109746596856	22.230635731620353
66-67	21.330709521346634	28.135940935382102	27.966313551027305	22.567035992243955
68-69	21.882772103042992	28.34264206705816	27.83654818602917	21.938037643869677
70-71	21.85518647840924	28.038880392661437	27.94489070473885	22.161042424190473
72-73	21.520316641350558	28.140130990981234	28.27839494465774	22.06115742301047
74-75	21.53143896521449	28.148685302415227	28.183987107711474	22.135888624658808
76-77	21.679378893598585	28.196227065776025	27.87890357186954	22.245490468755847
78-79	21.80302057948428	28.3075101377521	27.94792676190167	21.94154252086195
80-81	21.662451634556838	28.015537935659896	28.130453643397484	22.191556786385778
82-83	21.51243315917932	28.08431298400766	28.243990796333435	22.15926306047959
84-85	21.663690339307912	27.97286967576577	28.10894330735167	22.254496677574647
86-87	21.89244331079824	28.12851308037355	27.9710825704891	22.00796103833911
88-89	21.688597178102018	27.939732580739285	28.108200780398708	22.263469460759993
90-91	21.675485737124525	27.775897112410735	28.159041811848123	22.389575338616616
92-93	21.893754002708448	28.062924019217945	27.827546217115973	22.215775760957634
94-95	21.82114177106535	27.74468921982118	28.03742123168014	22.396747777433333
96-97	21.87278926597327	27.720719932410553	28.11722458116246	22.28926622045372
98-99	21.885115408452602	27.757154559445045	28.02324598415761	22.334484047944738
100	22.065706578814886	27.67588111732197	27.935207994591	22.323204309272143
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3047.0
1	2269.5
2	1906.5
3	2274.0
4	2063.5
5	1757.0
6	1468.5
7	1374.5
8	1411.5
9	1363.0
10	1207.0
11	1039.5
12	1026.5
13	1151.5
14	1311.5
15	1455.0
16	1771.5
17	2228.5
18	2897.5
19	4032.0
20	5570.0
21	7744.5
22	11079.5
23	16073.0
24	22697.5
25	30858.5
26	41561.5
27	54783.0
28	70909.0
29	89255.5
30	106308.5
31	122480.5
32	136869.0
33	149938.0
34	163815.0
35	176202.0
36	185807.0
37	195612.0
38	205154.0
39	209880.0
40	212370.5
41	214130.0
42	213309.5
43	211903.0
44	207885.5
45	203781.0
46	203106.0
47	200446.5
48	196336.5
49	192495.5
50	188651.0
51	182929.0
52	173271.0
53	163454.0
54	155636.0
55	148822.5
56	138623.0
57	126289.0
58	115594.5
59	104607.5
60	92079.5
61	77962.0
62	64885.0
63	53545.5
64	43333.0
65	34736.5
66	27223.5
67	20835.0
68	15834.0
69	11987.5
70	8933.0
71	6536.0
72	4944.5
73	3691.0
74	2711.0
75	1987.5
76	1394.0
77	999.5
78	730.5
79	530.0
80	378.0
81	261.0
82	209.5
83	156.5
84	114.0
85	101.0
86	78.0
87	60.0
88	52.5
89	44.0
90	34.0
91	26.5
92	20.5
93	19.0
94	23.5
95	21.0
96	19.5
97	19.0
98	15.5
99	21.5
100	46.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.019000056522284363
2	0.04182319466917408
3	0.04348755347988589
4	0.04617359789717328
5	0.049106824316051526
6	0.046997537902476155
7	0.04470698468773415
8	0.044492760286355404
9	0.04464106948730992
10-11	0.04530846089160526
12-13	0.04619831609733236
14-15	0.044558675486779636
16-17	0.04068615746185611
18-19	0.04422909948465849
20-21	0.04339692007930258
22-23	0.04446804208619632
24-25	0.04085094546291669
26-27	0.040974536463712116
28-29	0.04151833686721201
30-31	0.04157601266758322
32-33	0.04151009746715899
34-35	0.041246436665462065
36-37	0.0384203224472732
38-39	0.03901355925109127
40-41	0.040224751058886496
42-43	0.04001052665750775
44-45	0.038881728850242804
46-47	0.037958916044303584
48-49	0.037958916044303584
50-51	0.03933489585315939
52-53	0.039615035454962366
54-55	0.038922925850507946
56-57	0.03717617303926585
58-59	0.038115464645311126
60-61	0.037546946041652145
62-63	0.03787652204377329
64-65	0.03462195902282693
66-67	0.03568484162966764
68-69	0.0368465970371447
70-71	0.038098985845205074
72-73	0.0347290712235163
74-75	0.035281111027069226
76-77	0.036187445032902395
78-79	0.03651702103502355
80-81	0.036541739235182634
82-83	0.03501745022537231
84-85	0.03628631783353874
86-87	0.036821878836985615
88-89	0.03745631264106883
90-91	0.03769525524260667
92-93	0.03622040263311451
94-95	0.03748103084122792
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6068403.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.10146956274538
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.81455179899753	52.09757696842854
2	13.368482985811497	17.673527423733958
3	3.714984952987879	7.36697894387956
4	1.5044845478226245	3.977945581820718
5	0.7546880600551871	2.494299491555265
6	0.4383927584568113	1.7387043347796536
7	0.28564666700268204	1.3217165125204215
8	0.20431719050050126	1.0804533239211627
9	0.1510961475774022	0.898890966012216
>10	0.7140555294230226	8.22321122065302
>50	0.03714356952993194	1.6865614458488682
>100	0.012006082562545711	1.3070843812077162
>500	7.485689447959092E-5	0.028121954011988602
>1k	7.485237783344604E-5	0.1049274516268924
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	1.6478800106057557E-5	0.0	0.0	3.295760021211511E-5	0.0
10-11	1.6478800106057557E-5	0.0	0.0	4.943640031817267E-5	0.0
12-13	1.6478800106057557E-5	0.0	0.0	1.0711220068937412E-4	0.0
14-15	1.6478800106057557E-5	0.0	0.0	1.153516007424029E-4	0.0
16-17	1.6478800106057557E-5	0.0	0.0	1.235910007954317E-4	0.0
18-19	1.6478800106057557E-5	0.0	0.0	1.7302740111360438E-4	0.0
20-21	1.6478800106057557E-5	0.0	0.0	2.801396018029785E-4	0.0
22-23	1.6478800106057557E-5	0.0	0.0	6.261944040301872E-4	0.0
24-25	1.6478800106057557E-5	0.0	0.0	0.0013924586089618636	0.0
26-27	1.6478800106057557E-5	0.0	0.0	0.002760199017764641	0.0
28-29	1.6478800106057557E-5	8.239400053028778E-6	0.0	0.008453624454407527	0.0
30-31	1.6478800106057557E-5	1.6478800106057557E-5	0.0	0.026349601369586036	0.0
32-33	1.6478800106057557E-5	1.6478800106057557E-5	0.0	0.06774434723600262	0.0
34-35	1.6478800106057557E-5	1.6478800106057557E-5	0.0	0.13280265005471786	0.0
36-37	1.6478800106057557E-5	1.6478800106057557E-5	0.0	0.23085975008581333	0.0
38-39	1.6478800106057557E-5	1.6478800106057557E-5	0.0	0.3598228397158198	0.0
40-41	1.6478800106057557E-5	1.6478800106057557E-5	0.0	0.5022655878325814	0.0
42-43	1.6478800106057557E-5	1.6478800106057557E-5	0.0	0.6422777129336994	0.0
44-45	1.6478800106057557E-5	1.6478800106057557E-5	0.0	0.8029789715679727	0.0
46-47	1.6478800106057557E-5	1.6478800106057557E-5	0.0	0.9896755373695518	0.0
48-49	1.6478800106057557E-5	1.6478800106057557E-5	0.0	1.1856743858310004	0.0
50-51	1.6478800106057557E-5	1.6478800106057557E-5	0.0	1.3794897932783963	0.0
52-53	1.6478800106057557E-5	1.6478800106057557E-5	0.0	1.5684439546945055	0.0
54-55	1.6478800106057557E-5	3.295760021211511E-5	0.0	1.7778235888420726	0.0
56-57	2.4718200159086337E-5	3.295760021211511E-5	0.0	2.0105784009400827	0.0
58-59	3.295760021211511E-5	3.295760021211511E-5	0.0	2.2533111265023105	0.0
60-61	3.295760021211511E-5	3.295760021211511E-5	0.0	2.498004829277159	0.0
62-63	3.295760021211511E-5	3.295760021211511E-5	0.0	2.7363789122113347	0.0
64-65	3.295760021211511E-5	3.295760021211511E-5	0.0	2.994346288471613	0.0
66-67	3.295760021211511E-5	3.295760021211511E-5	0.0	3.2848840131415136	0.0
68-69	3.295760021211511E-5	3.295760021211511E-5	0.0	3.586487252082632	0.0
70-71	3.295760021211511E-5	3.295760021211511E-5	0.0	3.896914888480544	0.0
72-73	3.295760021211511E-5	4.943640031817267E-5	0.0	4.227809194610179	0.0
74-75	3.295760021211511E-5	4.943640031817267E-5	0.0	4.550381706686256	0.0
76-77	3.295760021211511E-5	4.943640031817267E-5	0.0	4.888633797063247	0.0
78-79	3.295760021211511E-5	4.943640031817267E-5	0.0	5.258162650041535	0.0
80-81	3.295760021211511E-5	4.943640031817267E-5	0.0	5.644318612326835	0.0
82-83	3.295760021211511E-5	4.943640031817267E-5	0.0	6.034932090040822	0.0
84-85	3.295760021211511E-5	4.943640031817267E-5	0.0	6.417536870903268	0.0
86-87	3.295760021211511E-5	4.943640031817267E-5	0.0	6.8182106560820035	1.6478800106057557E-5
88	3.295760021211511E-5	4.943640031817267E-5	0.0	7.137396774736286	1.6478800106057557E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5530	0.0	40.384407	1
GTATCAA	8825	0.0	33.457245	1
ATCAACG	9090	0.0	31.134766	3
TCAACGC	9100	0.0	30.997229	4
CAACGCA	9525	0.0	29.614151	5
AACGCAG	10305	0.0	27.596851	6
TATCAAC	10685	0.0	26.971119	2
GTACATG	14550	0.0	26.787783	1
TACATGG	14445	0.0	25.841396	2
ACATGGG	15185	0.0	23.436571	3
ACGCAGA	12105	0.0	23.182001	7
CGCAGAG	12285	0.0	22.765814	8
CATGGGG	10185	0.0	20.07896	4
GTGGTAT	3455	0.0	19.45957	1
TCTGTCG	1780	0.0	18.749071	8
GCAGAGT	14365	0.0	18.488062	9
GAGTACA	11255	0.0	18.2132	1
TACCTGG	4345	0.0	17.744635	2
TGGTATC	3490	0.0	16.838274	2
AGAGTAC	12405	0.0	16.767374	10-11
>>END_MODULE