FastQCFastQC Report
Wed 25 May 2016
SRR2049357_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049357_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6068403
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137180.22605617985489757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125890.2074516145351586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77380.12751295522067338No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90500.056.1600461
GTATCAA161950.038.815111
TCAACGC195700.031.7714354
ATCAACG195850.031.6991083
CAACGCA201100.030.9416685
TATCAAC204950.030.3833562
AACGCAG210600.029.5707666
TATAACG5450.028.4567452
GTACATG139700.026.4530961
ATAACGC6600.025.634593
ACGCAGA245500.025.2137457
CGCAGAG247400.024.887028
TACATGG139950.024.8164332
ACATGGG146050.023.2007223
GTGGTAT40350.022.9547751
TGGTATC39550.022.9339372
GCAGAGT273650.022.2930939
AGAGTAC257000.020.05872210-11
CAGAGTA270400.020.03879510-11
GAGTACT216050.019.35685712-13
Produced by FastQC (version 0.11.5)