##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049357_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6068403
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.795223224298056	31.0	31.0	34.0	30.0	34.0
2	31.901629308402885	33.0	31.0	34.0	30.0	34.0
3	31.955269944992118	33.0	31.0	34.0	30.0	34.0
4	35.70820230627399	37.0	35.0	37.0	33.0	37.0
5	35.76078500389641	37.0	35.0	37.0	35.0	37.0
6	35.844921637537915	37.0	35.0	37.0	35.0	37.0
7	35.792563051596936	37.0	35.0	37.0	35.0	37.0
8	35.801424361565964	37.0	35.0	37.0	35.0	37.0
9	37.54502065864775	39.0	37.0	39.0	35.0	39.0
10-11	37.50078084794303	39.0	37.0	39.0	35.0	39.0
12-13	37.44821223969469	39.0	37.0	39.0	34.5	39.0
14-15	38.74574653331362	40.0	38.0	41.0	35.0	41.0
16-17	38.77112083689893	40.0	38.0	41.0	35.0	41.0
18-19	38.83481420070487	40.0	38.0	41.0	35.0	41.0
20-21	38.856629660225266	40.0	38.0	41.0	35.0	41.0
22-23	38.81271580018664	40.0	38.0	41.0	35.0	41.0
24-25	38.78734470996736	40.0	38.0	41.0	35.0	41.0
26-27	38.672920371306915	40.0	38.0	41.0	34.0	41.0
28-29	38.586880601041166	40.0	38.0	41.0	34.0	41.0
30-31	38.483467808581594	40.0	38.0	41.0	34.0	41.0
32-33	38.33963383776589	40.0	38.0	41.0	34.0	41.0
34-35	38.22908374740439	40.0	38.0	41.0	34.0	41.0
36-37	38.10676523955314	40.0	38.0	41.0	33.5	41.0
38-39	38.00497107723399	40.0	38.0	41.0	33.0	41.0
40-41	37.89431544015781	40.0	37.5	41.0	33.0	41.0
42-43	37.75998092084524	40.0	37.0	41.0	33.0	41.0
44-45	37.597324205396376	40.0	37.0	41.0	33.0	41.0
46-47	37.382573965506246	40.0	37.0	41.0	32.5	41.0
48-49	37.1844623700832	40.0	37.0	41.0	32.0	41.0
50-51	37.04123432474738	40.0	37.0	41.0	31.0	41.0
52-53	36.90258845696306	40.0	36.0	41.0	31.0	41.0
54-55	36.753625294826335	39.5	36.0	41.0	31.0	41.0
56-57	36.60956078559713	39.0	35.5	41.0	31.0	41.0
58-59	36.41612109479216	39.0	35.0	41.0	30.5	41.0
60-61	36.23024682111587	39.0	35.0	41.0	30.0	41.0
62-63	36.0114511181937	39.0	35.0	41.0	30.0	41.0
64-65	35.77393475680505	38.0	35.0	40.0	30.0	41.0
66-67	35.497983901201025	37.5	35.0	40.0	29.5	41.0
68-69	35.19960045501263	37.0	35.0	40.0	29.0	41.0
70-71	34.855983856049114	36.5	34.0	39.5	29.0	41.0
72-73	34.54975501791823	36.0	34.0	39.0	29.0	41.0
74-75	34.239486154759334	36.0	34.0	39.0	29.0	40.5
76-77	33.912534483949074	35.0	34.0	38.5	28.5	40.0
78-79	33.57849247981718	35.0	34.0	37.0	28.0	39.0
80-81	33.26221602289762	35.0	34.0	37.0	28.0	39.0
82-83	32.96600299947119	35.0	33.5	36.0	27.5	39.0
84-85	32.69668321302986	35.0	33.0	36.0	27.0	37.0
86-87	32.46430494151427	35.0	33.0	36.0	27.0	37.0
88-89	32.236962014553086	35.0	33.0	35.0	26.0	37.0
90-91	32.042457958049255	35.0	33.0	35.0	26.0	36.0
92-93	31.858515082139405	35.0	33.0	35.0	26.0	36.0
94-95	31.697391801434414	35.0	33.0	35.0	25.5	36.0
96-97	31.48869785015926	35.0	33.0	35.0	25.0	36.0
98-99	31.334045629467916	35.0	33.0	35.0	25.0	35.0
100	31.219590887421287	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17701869636997003
1101	2	0.20597232319097358
1101	3	0.3762777959132677
1101	4	0.26076429828979286
1101	5	0.22921612833754068
1101	6	0.19859999291891484
1101	7	0.1137854099049278
1101	8	0.14922435182685234
1101	9	0.27714279952770227
1101	10-11	0.25839534094704675
1101	12-13	0.17870357440096996
1101	14-15	0.3139893452558695
1101	16-17	0.23650816239530315
1101	18-19	0.28377459472709887
1101	20-21	0.2750720887502993
1101	22-23	0.3219963167690736
1101	24-25	0.3448620902631063
1101	26-27	0.35540312058725476
1101	28-29	0.39814961325841836
1101	30-31	0.3806578540488488
1101	32-33	0.3757893530887131
1101	34-35	0.43579871537461656
1101	36-37	0.4133170914683788
1101	38-39	0.4398519180632121
1101	40-41	0.45864743656368745
1101	42-43	0.4747435068187613
1101	44-45	0.5049488965481288
1101	46-47	0.49887477031695227
1101	48-49	0.49478499224508
1101	50-51	0.4204551345926575
1101	52-53	0.4733220852418256
1101	54-55	0.4864914136798717
1101	56-57	0.424317880809447
1101	58-59	0.4232525047409865
1101	60-61	0.42964426427741387
1101	62-63	0.41828631621156376
1101	64-65	0.32303067590671475
1101	66-67	0.308540458617955
1101	68-69	0.368849130849938
1101	70-71	0.38512255875343726
1101	72-73	0.32812361405763113
1101	74-75	0.37279531310984027
1101	76-77	0.31711693298001364
1101	78-79	0.3838041191101027
1101	80-81	0.4541252598092669
1101	82-83	0.41371883663892817
1101	84-85	0.3969070257068097
1101	86-87	0.37209775964200986
1101	88-89	0.44726517622545003
1101	90-91	0.4580056435168913
1101	92-93	0.45008446910460975
1101	94-95	0.47789179121380343
1101	96-97	0.5073462545725498
1101	98-99	0.5329695782721942
1101	100	0.5371995560171285
1102	1	0.11113609177614947
1102	2	0.13710242620823365
1102	3	0.26326646524968567
1102	4	0.17318659633330213
1102	5	0.1331633511428265
1102	6	0.1509638290004247
1102	7	0.083633072226732
1102	8	0.14038457528471326
1102	9	0.13434509008054363
1102	10-11	0.1917001612693383
1102	12-13	0.16551359154536271
1102	14-15	0.21719090251900042
1102	16-17	0.17928649922931328
1102	18-19	0.2009635045148599
1102	20-21	0.1814049897010861
1102	22-23	0.24147824313278932
1102	24-25	0.19471767883612756
1102	26-27	0.2814740219505296
1102	28-29	0.29504123775006263
1102	30-31	0.2335597560290381
1102	32-33	0.27049119776848585
1102	34-35	0.2555501548586676
1102	36-37	0.2843617972173007
1102	38-39	0.3037512792528645
1102	40-41	0.3205694078622727
1102	42-43	0.3355984409873187
1102	44-45	0.3293166656874007
1102	46-47	0.24981830632360413
1102	48-49	0.24178332330789232
1102	50-51	0.3003100063389823
1102	52-53	0.3348851573026863
1102	54-55	0.23350972328589847
1102	56-57	0.32975177589711535
1102	58-59	0.33413551176383294
1102	60-61	0.27404908318206367
1102	62-63	0.27393473912422195
1102	64-65	0.2863722784705516
1102	66-67	0.24614864000002967
1102	68-69	0.3120550771046311
1102	70-71	0.28108550967402124
1102	72-73	0.23691839171787876
1102	74-75	0.2368999474001754
1102	76-77	0.24229608820832027
1102	78-79	0.3437059538663405
1102	80-81	0.29010336355455024
1102	82-83	0.31626278829073584
1102	84-85	0.2826438492430512
1102	86-87	0.24878673253795824
1102	88-89	0.24888670224546416
1102	90-91	0.291572717231368
1102	92-93	0.3178133654750184
1102	94-95	0.28682346333840414
1102	96-97	0.35571459923105664
1102	98-99	0.3273285751446764
1102	100	0.3019892799919752
1103	1	0.12792869202093016
1103	2	0.09976679229786711
1103	3	0.2804458331815596
1103	4	0.18227258376523992
1103	5	0.19033899225281203
1103	6	0.1402783369437799
1103	7	0.09628043801609465
1103	8	0.10120526098086202
1103	9	0.1319548435197433
1103	10-11	0.13111268148026767
1103	12-13	0.1849049390004538
1103	14-15	0.23455619088035462
1103	16-17	0.19449855192112153
1103	18-19	0.23747688731190664
1103	20-21	0.2574138777316364
1103	22-23	0.2332142761268301
1103	24-25	0.26237161405825304
1103	26-27	0.2847668395299223
1103	28-29	0.3169902972079086
1103	30-31	0.22999373678504043
1103	32-33	0.26063090148125667
1103	34-35	0.2554274156217744
1103	36-37	0.27604760821955665
1103	38-39	0.25241430768158324
1103	40-41	0.3202511678862052
1103	42-43	0.34113386522731304
1103	44-45	0.3379104070366239
1103	46-47	0.26395251989470836
1103	48-49	0.22536407017472726
1103	50-51	0.25336936561221535
1103	52-53	0.2512401925072041
1103	54-55	0.2496054306340909
1103	56-57	0.18618737472129254
1103	58-59	0.26854993283847506
1103	60-61	0.26479450401114235
1103	62-63	0.2502704242743192
1103	64-65	0.2615545404701507
1103	66-67	0.240635782410223
1103	68-69	0.2999696928251012
1103	70-71	0.30692604750881003
1103	72-73	0.28291576546460107
1103	74-75	0.2277934152247738
1103	76-77	0.19514621406047894
1103	78-79	0.17110443460833835
1103	80-81	0.21892532633583528
1103	82-83	0.1730269401722424
1103	84-85	0.20864147973121305
1103	86-87	0.19869687031855676
1103	88-89	0.26053939848656427
1103	90-91	0.33007419069928545
1103	92-93	0.33908455695723916
1103	94-95	0.31892140895091003
1103	96-97	0.39291849709811544
1103	98-99	0.41037156810073583
1103	100	0.4768799233350123
1104	1	0.12260248832033938
1104	2	0.17531391809633945
1104	3	0.25520224313808626
1104	4	0.18268975001727483
1104	5	0.14692812212993545
1104	6	0.11031130951359103
1104	7	0.15284701094500974
1104	8	0.14560952532166738
1104	9	0.17727494637921382
1104	10-11	0.15869128349500983
1104	12-13	0.16985851813250719
1104	14-15	0.21949029042462342
1104	16-17	0.1990456239176055
1104	18-19	0.21356882117466114
1104	20-21	0.24425600549012216
1104	22-23	0.2153677940328862
1104	24-25	0.22509976063531667
1104	26-27	0.25905933351890553
1104	28-29	0.23401501786686651
1104	30-31	0.27551365436566044
1104	32-33	0.24587472517617215
1104	34-35	0.2114403563885645
1104	36-37	0.23966578674703243
1104	38-39	0.2247420313858015
1104	40-41	0.2521871179654127
1104	42-43	0.2948944203727635
1104	44-45	0.22379141141556858
1104	46-47	0.2566471240203967
1104	48-49	0.2657550084051863
1104	50-51	0.2558541269146062
1104	52-53	0.19181145421434564
1104	54-55	0.1678261189769188
1104	56-57	0.18590466166129005
1104	58-59	0.2539029018035279
1104	60-61	0.24856103928643591
1104	62-63	0.26390385619974666
1104	64-65	0.17787365709778413
1104	66-67	0.23672395350047992
1104	68-69	0.20759567427447934
1104	70-71	0.2696119062839273
1104	72-73	0.2792005327403899
1104	74-75	0.25286264741531994
1104	76-77	0.205459308801494
1104	78-79	0.19836159752087212
1104	80-81	0.2499365775333331
1104	82-83	0.2132753108743941
1104	84-85	0.2656106610287452
1104	86-87	0.2514026122612094
1104	88-89	0.29500534939753464
1104	90-91	0.22449445576000215
1104	92-93	0.30404774662369505
1104	94-95	0.32699354808137926
1104	96-97	0.35663662507968397
1104	98-99	0.3626712124454272
1104	100	0.36703593955000713
1105	1	0.1739775005867159
1105	2	0.14480654648913216
1105	3	0.24897083719914548
1105	4	0.14095833378799938
1105	5	0.06874140964493591
1105	6	0.06692976124492134
1105	7	0.05942226564567932
1105	8	0.08540364209438422
1105	9	0.1326774734067513
1105	10-11	0.11228779484326168
1105	12-13	0.08287083630803238
1105	14-15	0.16831334843734425
1105	16-17	0.15237093277922753
1105	18-19	0.11273596780833373
1105	20-21	0.13806633132781343
1105	22-23	0.13972268829945733
1105	24-25	0.16257639610984143
1105	26-27	0.16599336417893085
1105	28-29	0.17358053512812432
1105	30-31	0.15429082817874473
1105	32-33	0.14212315167480227
1105	34-35	0.18293528733049413
1105	36-37	0.10047088678547311
1105	38-39	0.1363410132592051
1105	40-41	0.12456080238106182
1105	42-43	0.08636000919907616
1105	44-45	0.1004531262941839
1105	46-47	0.09267098188533396
1105	48-49	0.07288736908823523
1105	50-51	0.11065446933912426
1105	52-53	0.10694896861460279
1105	54-55	0.07031027012332913
1105	56-57	0.0726694831965844
1105	58-59	0.09688034693343184
1105	60-61	0.11562310332055148
1105	62-63	0.10505706629167832
1105	64-65	0.09085555167843751
1105	66-67	0.1507518828576906
1105	68-69	0.09830722399573233
1105	70-71	0.12382151769408267
1105	72-73	0.15737102427929983
1105	74-75	0.15021835978826914
1105	76-77	0.13173687359754638
1105	78-79	0.14345393114283667
1105	80-81	0.18292360137300534
1105	82-83	0.1425818502670353
1105	84-85	0.18284643409577228
1105	86-87	0.21618346718013726
1105	88-89	0.19199794224642375
1105	90-91	0.1623353417010449
1105	92-93	0.25556336735003526
1105	94-95	0.2293161049498842
1105	96-97	0.30108122073071897
1105	98-99	0.30555830770100556
1105	100	0.34048290202747467
1106	1	0.11861262919642002
1106	2	0.10313990396173622
1106	3	0.22514183816886657
1106	4	0.15664962174085417
1106	5	0.10580153053248154
1106	6	0.10572938229694984
1106	7	0.15125255071987453
1106	8	0.13017405201883037
1106	9	0.21076335118845435
1106	10-11	0.15484214707213795
1106	12-13	0.1355325556789353
1106	14-15	0.21390744467599632
1106	16-17	0.21490514416414186
1106	18-19	0.20116257915551472
1106	20-21	0.17716803727922326
1106	22-23	0.18606615206500976
1106	24-25	0.20196159368545352
1106	26-27	0.24890558185685308
1106	28-29	0.23014372831752894
1106	30-31	0.27415522194938546
1106	32-33	0.20610541167467034
1106	34-35	0.23579488839327212
1106	36-37	0.2617606691525509
1106	38-39	0.3072059762821837
1106	40-41	0.351828565192811
1106	42-43	0.36005394296110893
1106	44-45	0.36471500718579364
1106	46-47	0.34961445070082675
1106	48-49	0.3352480951377075
1106	50-51	0.3666857089863882
1106	52-53	0.33184312876439037
1106	54-55	0.3449179417581547
1106	56-57	0.3417528580253304
1106	58-59	0.34154015432906704
1106	60-61	0.27629400903669676
1106	62-63	0.30205842905020575
1106	64-65	0.2559269624074929
1106	66-67	0.2295416618452606
1106	68-69	0.2812172920713749
1106	70-71	0.32439360398483785
1106	72-73	0.3215554132761511
1106	74-75	0.36360427232087034
1106	76-77	0.3397843858668068
1106	78-79	0.331427501438867
1106	80-81	0.32719070020990415
1106	82-83	0.3117922290537507
1106	84-85	0.26277264782083876
1106	86-87	0.30000531301959654
1106	88-89	0.31855587886769854
1106	90-91	0.3162273903403374
1106	92-93	0.3655682620704539
1106	94-95	0.3426199692999141
1106	96-97	0.4026432839607459
1106	98-99	0.40254798825828075
1106	100	0.43078657118734043
1107	1	0.07893336750036184
1107	2	0.019872271121865737
1107	3	0.1612111124887292
1107	4	0.15698698838850333
1107	5	0.12315568932113763
1107	6	0.07488595764274919
1107	7	0.0887991674266786
1107	8	0.10406967696268765
1107	9	0.15070077972506368
1107	10-11	0.12780234792245437
1107	12-13	0.11613077713481346
1107	14-15	0.15779048315332744
1107	16-17	0.07952876240070594
1107	18-19	0.09332732590885229
1107	20-21	0.09633476517391415
1107	22-23	0.10628758941306415
1107	24-25	0.09923587181287274
1107	26-27	0.18233089824428816
1107	28-29	0.2064873854334337
1107	30-31	0.19069182286892072
1107	32-33	0.20117923353765832
1107	34-35	0.17871776539214324
1107	36-37	0.12935976881808386
1107	38-39	0.10412612518528874
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2216	66-67	-0.447656379651562
2216	68-69	-0.4553600652011127
2216	70-71	-0.5365708517500138
2216	72-73	-0.5198911862341475
2216	74-75	-0.5282999381881552
2216	76-77	-0.48313585857814445
2216	78-79	-0.7044835491838057
2216	80-81	-0.8096992656341016
2216	82-83	-0.7395422858595637
2216	84-85	-0.854957863868627
2216	86-87	-0.7183611434728476
2216	88-89	-1.014122326373844
2216	90-91	-0.7763100987916829
2216	92-93	-0.9081643253185447
2216	94-95	-0.8743936887229609
2216	96-97	-0.9921076876059729
2216	98-99	-1.1782621730406575
2216	100	-1.186740262510959
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	394.0
3	31.0
4	37.0
5	119.0
6	309.0
7	888.0
8	1955.0
9	3436.0
10	5349.0
11	7713.0
12	11452.0
13	16041.0
14	17658.0
15	20433.0
16	24823.0
17	33104.0
18	32696.0
19	19242.0
20	15538.0
21	15287.0
22	16482.0
23	18394.0
24	21639.0
25	26690.0
26	33971.0
27	43494.0
28	56692.0
29	73896.0
30	96014.0
31	124295.0
32	159549.0
33	209371.0
34	284985.0
35	412701.0
36	669566.0
37	1232120.0
38	1689661.0
39	669720.0
40	2658.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.70658517976816	20.185003966419625	13.113484441941575	23.99492641187064
2	16.19961627466073	20.70165742123587	38.85277559845646	24.245950705646937
3	17.77841715522189	25.219155682310486	30.845182826519597	26.157244335948025
4	12.599717421302953	16.889449241793304	37.990174672129285	32.52065866477446
5	12.227302636294919	37.35262803080152	35.5715663577386	14.848502975164966
6	30.742025537855678	37.9690175487686	17.353099324484546	13.93585758889118
7	26.41999880363911	32.54027459942921	22.776700888190845	18.263025708740834
8	24.82186220407044	35.67402991528485	20.903753516614767	18.600354364029943
9	24.6185363760449	15.984930466879014	22.00771768124167	37.38881547583441
10-11	23.565153797465328	26.471264350769058	29.216583012037926	20.746998839727684
12-13	24.38554923577055	24.138738560215558	29.393830936583125	22.081881267430767
14-15	21.69968265921934	26.30671441677972	26.93288467012914	25.0607182538718
16-17	20.701921082037565	29.508331928515624	27.491021937732217	22.298725051714595
18-19	20.46027783270714	27.799988464834225	30.477844242304396	21.261889460154244
20-21	21.746866162464446	27.347627219932903	30.03327813755145	20.872228480051202
22-23	21.271650877504346	27.37459097558287	30.10095901672977	21.252799130183014
24-25	21.12957507559245	27.319941328822097	30.191210298205608	21.35927329737985
26-27	20.899988761449066	27.150097933589723	30.381626254573284	21.568287050387926
28-29	21.0433084571478	27.1838688613738	30.36568610689413	21.40713657458427
30-31	21.432255029227214	27.777359857062876	29.789174333772355	21.001210779937555
32-33	20.86842774824039	27.4261451798104	30.369824493869217	21.335602578079992
34-35	20.947073519720767	26.94358100052419	30.482323261868576	21.627022217886463
36-37	21.596905411385656	27.57933949470532	29.625785184380167	21.19796990952886
38-39	21.432149691153565	27.598194116790157	29.779671306449096	21.18998488560718
40-41	21.089180732437367	27.30721594305975	30.030283125886974	21.57332019861591
42-43	21.09871213516298	27.31216045804665	29.895165552901084	21.69396185388928
44-45	21.285991306593964	27.54235161947748	29.694255933511222	21.477401140417335
46-47	21.28254968178167	27.979359951183152	29.141314348856405	21.596776018178772
48-49	21.282964848151675	27.627316977673367	29.305711693344392	21.784006480830563
50-51	21.357915391786854	27.519524354306935	29.135407993810542	21.98715226009567
52-53	21.250423610074886	27.682441536454032	29.071017441158673	21.996117412312405
54-55	21.433697532734854	28.165487027095704	28.82826698859778	21.572548451571663
56-57	21.12732595102019	27.856315147144755	28.841734974320982	22.174623927514073
58-59	20.932879133135014	27.38682511820257	29.310241124672814	22.370054623989603
60-61	21.394530874076693	27.82936567051041	28.76002407227483	22.01607938313807
62-63	21.203928938584937	27.868979022573903	28.805731832004266	22.121360206836894
64-65	21.1352006408299	27.57187625496817	29.036055910134845	22.256867194067084
66-67	21.05723963372738	27.65541509295445	28.71247840115261	22.574866872165554
68-69	21.60632439309538	27.841920164474033	28.50429530379404	22.047460138636545
70-71	21.625284826722773	27.590581180405156	28.51570414356709	22.268429849304983
72-73	21.30225854657844	27.778467604741618	28.679404506942124	22.239869341737815
74-75	21.365750725382814	27.72887511797899	28.56036177888626	22.34501237775193
76-77	21.471236486269365	27.847509172569545	28.23613938845142	22.445114952709666
78-79	21.5901274476296	27.943969712360367	28.26414608044734	22.20175675956269
80-81	21.41940899678293	27.686125079058932	28.38420021034043	22.510265713817706
82-83	21.24646639273666	27.7205614024333	28.514221808819663	22.518750396010372
84-85	21.3688318883526	27.654213811665873	28.352891700024735	22.62406259995679
86-87	21.552979667545983	27.800977468587725	28.220705934155177	22.42533692971112
88-89	21.27316599095545	27.659191066357575	28.306745013737377	22.760897928949593
90-91	21.1348718022663	27.47479200182919	28.364074560057347	23.026261635847163
92-93	21.292712380344692	27.744631743114674	28.113246226597923	22.849409649942718
94-95	21.190486078083755	27.446012838728834	28.3500615173984	23.01343956578901
96-97	21.17375752236481	27.449170582959997	28.418035021039056	22.959036873636133
98-99	21.14105384583694	27.51046018209533	28.307230255839823	23.04125571622791
100	21.29409573968456	27.49173166258262	28.125827039729216	23.088345558003603
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1361.0
1	1017.5
2	1903.5
3	2956.5
4	1766.5
5	832.0
6	1580.5
7	3458.0
8	5389.5
9	5924.5
10	4549.0
11	3071.5
12	2953.5
13	3314.0
14	3805.5
15	4411.5
16	5002.5
17	5660.0
18	6489.0
19	7660.0
20	9206.5
21	11239.5
22	14503.0
23	19171.0
24	25187.0
25	32696.0
26	42960.5
27	55902.5
28	71492.0
29	89121.0
30	105515.5
31	120871.0
32	135401.5
33	148760.5
34	161847.5
35	173271.5
36	182627.0
37	191779.0
38	200478.0
39	205728.5
40	208860.0
41	211138.5
42	210790.5
43	209737.5
44	207414.0
45	203999.5
46	202516.0
47	200641.5
48	196414.5
49	192604.5
50	189310.0
51	182742.0
52	172124.5
53	161820.0
54	154245.5
55	147955.5
56	138625.0
57	127224.5
58	116328.5
59	104821.0
60	91809.0
61	77064.0
62	63944.0
63	52162.5
64	41540.5
65	33485.0
66	26254.5
67	19880.0
68	14890.5
69	10892.0
70	7886.0
71	5667.0
72	4131.5
73	2956.0
74	2124.0
75	1519.0
76	985.5
77	648.5
78	446.0
79	267.5
80	149.0
81	87.5
82	49.5
83	26.5
84	13.0
85	7.0
86	4.0
87	1.5
88	2.0
89	2.0
90	0.5
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.5
97	0.5
98	1.0
99	2.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04085094546291669
2	0.0
3	0.0
4	8.239400053028778E-5
5	0.0
6	0.0
7	0.0
8	0.0011040796071058563
9	0.0
10-11	0.0
12-13	0.043174456277870796
14-15	9.063340058331657E-5
16-17	0.0
18-19	4.943640031817267E-5
20-21	7.415460047725902E-5
22-23	0.0
24-25	9.887280063634535E-5
26-27	8.239400053028778E-5
28-29	2.5542140164389217E-4
30-31	8.239400053028778E-6
32-33	1.8126680116663314E-4
34-35	6.591520042423023E-5
36-37	5.767580037120144E-5
38-39	2.471820015908633E-4
40-41	1.1535160074240289E-4
42-43	4.202094027044677E-4
44-45	1.7302740111360435E-4
46-47	1.0711220068937414E-4
48-49	7.4154600477259E-4
50-51	3.378154021741799E-4
52-53	4.5316700291658286E-4
54-55	3.954912025453814E-4
56-57	4.119700026514389E-4
58-59	1.1535160074240289E-4
60-61	1.8126680116663314E-4
62-63	7.662642049316763E-4
64-65	3.625336023332663E-4
66-67	0.001343022208643691
68-69	0.0014748526094921514
70-71	3.378154021741799E-4
72-73	8.816158056740793E-4
74-75	9.145734058861945E-4
76-77	6.261944040301872E-4
78-79	0.003048578019620648
80-81	0.0023894260153783458
82-83	0.0010628826068407125
84-85	0.002570692816544979
86-87	0.001235910007954317
88-89	0.001738513411189072
90-91	0.002315271414901087
92-93	0.0022905532147420005
94-95	0.003312238821317569
96-97	0.002719002017499497
98-99	0.002521256416226806
100	0.0012029524077422015
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6068403.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.07351724219077
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.85682833256085	49.88560832588626
2	13.86825599887938	17.771758797266273
3	3.9997595339579894	7.688359843910228
4	1.5243785335520765	3.9068917701269785
5	0.7905959705048485	2.5328132273874484
6	0.46821644265363627	1.8000164586866998
7	0.2984082307486413	1.33840454426593
8	0.2151311239650211	1.102736622456373
9	0.15616477745349275	0.9005423904710329
>10	0.7676081430258481	8.608213113400701
>50	0.038423105192509015	1.684111566559717
>100	0.01548344558269563	1.6707400325985082
>500	3.8605525553546535E-4	0.1737932471859336
>1k	2.830980962412072E-4	0.37460520338882736
>5k	2.5736190460734527E-5	0.12760025786146081
>10k+	5.1472380921469055E-5	0.4338045985476156
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13718	0.22605617985489757	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12589	0.2074516145351586	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7738	0.12751295522067338	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	3.295760021211511E-5	0.0
7	0.0	0.0	0.0	3.295760021211511E-5	0.0
8	0.0	0.0	0.0	3.295760021211511E-5	0.0
9	0.0	0.0	0.0	3.295760021211511E-5	0.0
10-11	0.0	0.0	0.0	3.295760021211511E-5	0.0
12-13	0.0	0.0	0.0	8.239400053028779E-5	0.0
14-15	0.0	0.0	0.0	9.063340058331657E-5	0.0
16-17	0.0	0.0	0.0	1.0711220068937412E-4	0.0
18-19	0.0	0.0	0.0	1.3183040084846045E-4	0.0
20-21	0.0	0.0	0.0	2.471820015908634E-4	0.0
22-23	0.0	0.0	0.0	5.93236803818072E-4	0.0
24-25	0.0	0.0	0.0	0.0013595010087497487	0.0
26-27	4.943640031817267E-5	0.0	0.0	0.002719002017499497	0.0
28-29	6.591520042423023E-5	0.0	0.0	0.008461863854460554	0.0
30-31	6.591520042423023E-5	0.0	0.0	0.026291925569214832	0.0
32-33	6.591520042423023E-5	0.0	0.0	0.06806568383807074	0.0
34-35	6.591520042423023E-5	0.0	0.0	0.13361835065996772	0.0
36-37	6.591520042423023E-5	0.0	0.0	0.23207094189360858	0.0
38-39	6.591520042423023E-5	0.0	0.0	0.36178381692844064	0.0
40-41	6.591520042423023E-5	0.0	0.0	0.5052729688519368	0.0
42-43	6.591520042423023E-5	0.0	0.0	0.6462491037592593	0.0
44-45	6.591520042423023E-5	0.0	0.0	0.8078896539995779	0.0
46-47	6.591520042423023E-5	0.0	0.0	0.9960033966102778	0.0
48-49	6.591520042423023E-5	0.0	0.0	1.1932628732798398	0.0
50-51	6.591520042423023E-5	0.0	0.0	1.3880999663338114	0.0
52-53	6.591520042423023E-5	0.0	0.0	1.5787432047607912	0.0
54-55	6.591520042423023E-5	0.0	0.0	1.7893010731159418	0.0
56-57	6.591520042423023E-5	0.0	0.0	2.0234565832229663	0.0
58-59	6.591520042423023E-5	0.0	0.0	2.267169797391505	0.0
60-61	6.591520042423023E-5	0.0	0.0	2.5134619437766412	0.0
62-63	6.591520042423023E-5	0.0	0.0	2.753648694722483	0.0
64-65	8.239400053028779E-5	0.0	0.0	3.013642963395806	0.0
66-67	8.239400053028779E-5	1.6478800106057557E-5	0.0	3.306207580478752	0.0
68-69	8.239400053028779E-5	1.6478800106057557E-5	0.0	3.6104309486367336	0.0
70-71	8.239400053028779E-5	1.6478800106057557E-5	0.0	3.923956599454585	0.0
72-73	9.063340058331657E-5	1.6478800106057557E-5	0.0	4.257891244203789	0.0
74-75	9.887280063634535E-5	1.6478800106057557E-5	0.0	4.584138528703516	0.0
76-77	9.887280063634535E-5	1.6478800106057557E-5	0.0	4.926386728106225	0.0
78-79	9.887280063634535E-5	1.6478800106057557E-5	0.0	5.300027041710974	0.0
80-81	9.887280063634535E-5	1.6478800106057557E-5	0.0	5.689989606820773	0.0
82-83	9.887280063634535E-5	1.6478800106057557E-5	0.0	6.084673348160957	0.0
84-85	9.887280063634535E-5	1.6478800106057557E-5	0.0	6.470648043645091	0.0
86-87	9.887280063634535E-5	1.6478800106057557E-5	0.0	6.875482725850606	0.0
88	9.887280063634535E-5	1.6478800106057557E-5	0.0	7.1984342503291225	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9050	0.0	56.160046	1
GTATCAA	16195	0.0	38.81511	1
TCAACGC	19570	0.0	31.771435	4
ATCAACG	19585	0.0	31.699108	3
CAACGCA	20110	0.0	30.941668	5
TATCAAC	20495	0.0	30.383356	2
AACGCAG	21060	0.0	29.570766	6
TATAACG	545	0.0	28.456745	2
GTACATG	13970	0.0	26.453096	1
ATAACGC	660	0.0	25.63459	3
ACGCAGA	24550	0.0	25.213745	7
CGCAGAG	24740	0.0	24.88702	8
TACATGG	13995	0.0	24.816433	2
ACATGGG	14605	0.0	23.200722	3
GTGGTAT	4035	0.0	22.954775	1
TGGTATC	3955	0.0	22.933937	2
GCAGAGT	27365	0.0	22.293093	9
AGAGTAC	25700	0.0	20.058722	10-11
CAGAGTA	27040	0.0	20.038795	10-11
GAGTACT	21605	0.0	19.356857	12-13
>>END_MODULE