FastQCFastQC Report
Wed 25 May 2016
SRR2049356_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049356_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3868144
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35150.040.6522561
GTATCAA60900.035.8898851
TCAACGC62650.033.4610024
ATCAACG64200.033.019213
CAACGCA66950.031.5225185
AACGCAG71000.029.8556486
TATCAAC74600.028.4156282
ACGCAGA84700.024.5271537
GTACATG101950.023.8824541
CGCAGAG86250.023.6504258
TACATGG104800.022.4247842
ACATGGG109650.020.7473143
GCAGAGT99150.019.5304749
TACACCG4900.019.1848775
GAGTACA78000.018.6208821
AGAGTAC85100.017.36998410-11
ATACCGT4900.017.265726
TAGACTG15150.017.063785
GTATAGG13050.016.9287031
CATGGGG80700.016.8907764
Produced by FastQC (version 0.11.5)