##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049356_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3868144
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.727106074644585	31.0	31.0	34.0	28.0	34.0
2	30.832369994498652	31.0	31.0	34.0	28.0	34.0
3	31.031078470708433	31.0	31.0	34.0	28.0	34.0
4	34.65286788702799	37.0	35.0	37.0	32.0	37.0
5	34.660815626305535	37.0	35.0	37.0	32.0	37.0
6	34.697233608676406	37.0	35.0	37.0	32.0	37.0
7	34.55303939046737	37.0	35.0	37.0	31.0	37.0
8	34.54429100881456	37.0	35.0	37.0	31.0	37.0
9	36.1163664020781	39.0	37.0	39.0	32.0	39.0
10-11	36.10696124549655	39.0	37.0	39.0	32.0	39.0
12-13	36.06703654258993	39.0	37.0	39.0	31.5	39.0
14-15	37.25836189138771	40.0	37.5	41.0	31.5	41.0
16-17	37.212140240901064	40.0	37.0	41.0	31.0	41.0
18-19	37.20719523368313	40.0	37.0	41.0	31.0	41.0
20-21	37.29384880707647	40.0	38.0	41.0	31.0	41.0
22-23	37.22728755702994	40.0	37.0	41.0	31.0	41.0
24-25	37.22640677286058	40.0	37.0	41.0	31.0	41.0
26-27	37.209493364259444	40.0	37.0	41.0	31.0	41.0
28-29	37.160526857324854	40.0	37.0	41.0	31.0	41.0
30-31	37.09945842243722	40.0	37.0	41.0	31.0	41.0
32-33	37.05732671792984	40.0	37.0	41.0	31.0	41.0
34-35	36.967671705086474	40.0	37.0	41.0	30.0	41.0
36-37	36.87317935940337	40.0	37.0	41.0	30.0	41.0
38-39	36.76311675573608	40.0	37.0	41.0	30.0	41.0
40-41	36.66724480784583	40.0	37.0	41.0	30.0	41.0
42-43	36.521738590910786	40.0	36.0	41.0	30.0	41.0
44-45	36.369047662134605	40.0	36.0	41.0	30.0	41.0
46-47	36.24554023841925	40.0	36.0	41.0	30.0	41.0
48-49	36.11854186400506	39.0	36.0	41.0	29.0	41.0
50-51	35.984194745593754	39.0	35.0	41.0	29.0	41.0
52-53	35.83254876240388	39.0	35.0	41.0	28.0	41.0
54-55	35.69949658027208	39.0	35.0	41.0	28.0	41.0
56-57	35.55930298872017	39.0	35.0	40.5	28.0	41.0
58-59	35.37455236930166	39.0	35.0	40.0	28.0	41.0
60-61	35.15090583494306	38.0	34.0	40.0	27.5	41.0
62-63	34.93223171112554	38.0	34.0	40.0	27.0	41.0
64-65	34.69038652645817	38.0	34.0	40.0	26.5	41.0
66-67	34.432084353633165	37.0	34.0	40.0	26.0	41.0
68-69	34.14338375200096	37.0	34.0	40.0	26.0	41.0
70-71	33.859303195537706	36.0	34.0	39.0	26.0	41.0
72-73	33.51998115892273	36.0	33.0	39.0	26.0	41.0
74-75	33.17956932316894	35.0	33.0	39.0	25.0	40.0
76-77	32.856037288167144	35.0	33.0	37.5	25.0	40.0
78-79	32.53928848047022	35.0	33.0	37.0	25.0	39.0
80-81	32.198797666270956	35.0	33.0	37.0	24.0	39.0
82-83	31.886091624303543	35.0	32.0	36.0	23.5	38.5
84-85	31.60639547028239	35.0	32.0	36.0	23.0	37.0
86-87	31.301892587245977	35.0	32.0	35.5	21.0	37.0
88-89	31.058775604010606	35.0	32.0	35.0	20.0	36.5
90-91	30.864206063683255	35.0	32.0	35.0	19.5	36.0
92-93	30.636188052978376	35.0	32.0	35.0	18.0	36.0
94-95	30.43820692818054	35.0	32.0	35.0	15.0	36.0
96-97	30.190958247676406	34.0	31.5	35.0	4.5	35.5
98-99	29.9945173964568	34.0	31.0	35.0	2.0	35.0
100	29.872810319367634	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.28351036759683623
1101	2	0.284141005205079
1101	3	0.27224881617267727
1101	4	0.2734799877189644
1101	5	0.28067690574408033
1101	6	0.3631786945802844
1101	7	0.3405630938180835
1101	8	0.46980506441691006
1101	9	0.5426506954479393
1101	10-11	0.5255761864769966
1101	12-13	0.5481635073575859
1101	14-15	0.6363817688853857
1101	16-17	0.6464555276759256
1101	18-19	0.562535022051847
1101	20-21	0.5460285489166239
1101	22-23	0.550574469708593
1101	24-25	0.566490047733943
1101	26-27	0.5113975178333163
1101	28-29	0.5156960205422365
1101	30-31	0.502758916266032
1101	32-33	0.5032855521778927
1101	34-35	0.4603822437988896
1101	36-37	0.44390986607010774
1101	38-39	0.581940537400655
1101	40-41	0.6023908195358771
1101	42-43	0.5146915076510439
1101	44-45	0.44483948237706983
1101	46-47	0.43635373140382683
1101	48-49	0.3282841396220135
1101	50-51	0.3783199339001442
1101	52-53	0.34897973305663044
1101	54-55	0.35015741129509337
1101	56-57	0.3755156604613532
1101	58-59	0.4154213352987526
1101	60-61	0.37489173661626296
1101	62-63	0.45474649575304227
1101	64-65	0.3802180004151694
1101	66-67	0.35727789979532787
1101	68-69	0.38077058512234174
1101	70-71	0.39108337473506793
1101	72-73	0.4770866389527413
1101	74-75	0.5016534692767323
1101	76-77	0.48385265393496013
1101	78-79	0.4515370566476662
1101	80-81	0.45516171269998296
1101	82-83	0.5443890833514828
1101	84-85	0.5222488718471539
1101	86-87	0.547939076515739
1101	88-89	0.5836840489487791
1101	90-91	0.5628272436643194
1101	92-93	0.5831911188196024
1101	94-95	0.5055801142557357
1101	96-97	0.4880829173712975
1101	98-99	0.49589270388420914
1101	100	0.5437619001853058
1102	1	0.007189964574898511
1102	2	0.07976659197851532
1102	3	0.07930687297759675
1102	4	0.1730496027045163
1102	5	0.033140223354024556
1102	6	0.22108254603619315
1102	7	0.1698921149225896
1102	8	0.20759718674276684
1102	9	0.2594914353244988
1102	10-11	0.2303350780355231
1102	12-13	0.3249489424622851
1102	14-15	0.3001624801736398
1102	16-17	0.2721032438867752
1102	18-19	0.2886974110062184
1102	20-21	0.23592230600245756
1102	22-23	0.20368929974063832
1102	24-25	0.22509825802965366
1102	26-27	0.2843925855212319
1102	28-29	0.29805516197980353
1102	30-31	0.21010002076709355
1102	32-33	0.2644869557408711
1102	34-35	0.2684619534884547
1102	36-37	0.2282886192115754
1102	38-39	0.2351189057784424
1102	40-41	0.2866627383981708
1102	42-43	0.23065839036022595
1102	44-45	0.19758199834620171
1102	46-47	0.06079726997907642
1102	48-49	0.12298453461620795
1102	50-51	0.14401936965656148
1102	52-53	0.14068682838643554
1102	54-55	0.10339238739065593
1102	56-57	0.0306900982653886
1102	58-59	0.07835853178779217
1102	60-61	0.04199986273049916
1102	62-63	0.008357832572514212
1102	64-65	0.008939706650167523
1102	66-67	0.007040528064315765
1102	68-69	0.07240681986608166
1102	70-71	0.046895503170084396
1102	72-73	0.05871198080148332
1102	74-75	-0.020217245945246987
1102	76-77	-0.004885606877536475
1102	78-79	0.03075952276242333
1102	80-81	0.04879986980502338
1102	82-83	0.06613366296594592
1102	84-85	0.04689998925685046
1102	86-87	-0.019001818979681673
1102	88-89	-0.008604548228369424
1102	90-91	0.03855932956961894
1102	92-93	0.06421089742929453
1102	94-95	0.07093142787839923
1102	96-97	0.11691459382007707
1102	98-99	0.17628761489075373
1102	100	0.1581218152546029
1103	1	0.057180382402776786
1103	2	0.0253588680893877
1103	3	0.12257923007767957
1103	4	0.12623770252952227
1103	5	0.07548492108542604
1103	6	0.15312526761399425
1103	7	0.11133853310192876
1103	8	0.17542160792775263
1103	9	0.21772116600420333
1103	10-11	0.22958918616854618
1103	12-13	0.17149576293932967
1103	14-15	0.302383548250873
1103	16-17	0.24497303077400545
1103	18-19	0.2559678347248209
1103	20-21	0.20865793362262508
1103	22-23	0.23354330912605548
1103	24-25	0.265755040862679
1103	26-27	0.28670943103296764
1103	28-29	0.26814279744664304
1103	30-31	0.2842023339190902
1103	32-33	0.3186566975058298
1103	34-35	0.260692378170738
1103	36-37	0.2556806997581518
1103	38-39	0.23441133669505376
1103	40-41	0.18256245446413288
1103	42-43	0.16566384988066574
1103	44-45	0.0979900149410966
1103	46-47	0.07394577864853602
1103	48-49	0.1428303778238842
1103	50-51	0.014393794176335462
1103	52-53	0.09745790180612346
1103	54-55	0.10253666443355058
1103	56-57	0.08221146405869462
1103	58-59	0.1017467376588499
1103	60-61	0.06292841314818531
1103	62-63	0.07948088883163251
1103	64-65	0.06405111715268674
1103	66-67	0.09950249558023216
1103	68-69	0.1409975904348073
1103	70-71	0.0961553818640013
1103	72-73	0.13577695808490375
1103	74-75	0.11478484187881577
1103	76-77	0.20235726045675761
1103	78-79	0.102729429870422
1103	80-81	0.07088427434823785
1103	82-83	0.12502493110198998
1103	84-85	0.10774746130092439
1103	86-87	0.09384874038090629
1103	88-89	0.09811993112281314
1103	90-91	0.08898700504390078
1103	92-93	0.10163039411887809
1103	94-95	0.16630446548541755
1103	96-97	0.1459924192152755
1103	98-99	0.14164237182141548
1103	100	0.15010210848648953
1104	1	-0.1305417785887002
1104	2	-0.1356148977120064
1104	3	-0.03351621394393689
1104	4	-0.049139943350660076
1104	5	0.03407687934669923
1104	6	0.010904630354239941
1104	7	-0.009066862136748455
1104	8	0.06415908472890663
1104	9	0.0811288079130108
1104	10-11	0.09106441123581277
1104	12-13	0.06877028868677115
1104	14-15	0.16866764200490536
1104	16-17	0.12221403711561152
1104	18-19	0.17804689082016978
1104	20-21	0.11199747692957374
1104	22-23	0.13981023938010395
1104	24-25	0.11040108157590112
1104	26-27	0.05221503240650094
1104	28-29	0.14082059956086823
1104	30-31	0.09494730264323437
1104	32-33	0.12083360283639166
1104	34-35	0.08298530502070633
1104	36-37	0.029952295922562655
1104	38-39	0.12810697374582958
1104	40-41	0.09324019452112964
1104	42-43	0.0022451000108389962
1104	44-45	-0.04182319846027127
1104	46-47	-0.08634827628287667
1104	48-49	-0.026870604083036653
1104	50-51	0.01006841932869662
1104	52-53	-0.008765843415787344
1104	54-55	-0.02262584262758338
1104	56-57	-0.05671593195612701
1104	58-59	0.022321146758535804
1104	60-61	0.047172478854605515
1104	62-63	-0.014617510984408
1104	64-65	-0.008619317436370011
1104	66-67	0.06744058456605018
1104	68-69	0.036203244273622204
1104	70-71	0.04613121779828333
1104	72-73	0.06819701583213345
1104	74-75	0.1389377996355563
1104	76-77	0.13868158231503003
1104	78-79	0.10555636344552255
1104	80-81	0.07734154178132968
1104	82-83	0.030291790656132633
1104	84-85	0.07610197131020158
1104	86-87	0.0940167057864123
1104	88-89	0.10931856937746787
1104	90-91	0.12158212652376577
1104	92-93	0.08636601543715372
1104	94-95	0.10982174466970918
1104	96-97	0.13514756385183802
1104	98-99	0.11349105815625649
1104	100	0.09622957432278056
1105	1	-0.03354453442488747
1105	2	0.021293324343691467
1105	3	0.0565640042298412
1105	4	0.04329276797447079
1105	5	0.08921981019691572
1105	6	0.13300373399087562
1105	7	0.05492996025718355
1105	8	0.13944500785213165
1105	9	0.20848529354621803
1105	10-11	0.23078796769769383
1105	12-13	0.2197962541507792
1105	14-15	0.21543311747878136
1105	16-17	0.2234128053837665
1105	18-19	0.12809275317577118
1105	20-21	0.2177501314181498
1105	22-23	0.2192688178927753
1105	24-25	0.14774450462746813
1105	26-27	0.16659738522724155
1105	28-29	0.2112129683924806
1105	30-31	0.18683703643683458
1105	32-33	0.255479336613611
1105	34-35	0.2467605958576513
1105	36-37	0.24713859945764938
1105	38-39	0.21940520599771673
1105	40-41	0.27644146883943677
1105	42-43	0.19146109942118272
1105	44-45	0.23855856470161285
1105	46-47	0.13862909965331482
1105	48-49	0.15085298513766787
1105	50-51	0.1203413822416195
1105	52-53	0.09612852984645315
1105	54-55	0.13704538161067603
1105	56-57	0.09315200902884868
1105	58-59	0.07385917361258265
1105	60-61	0.06229251947554815
1105	62-63	0.08144753985319397
1105	64-65	0.09485025166223693
1105	66-67	0.11480329437609527
1105	68-69	0.1186166688402821
1105	70-71	0.1497183091831218
1105	72-73	0.14897147541388023
1105	74-75	0.2032300656863555
1105	76-77	0.2229536607396554
1105	78-79	0.14412803770814264
1105	80-81	0.18122735270556944
1105	82-83	0.13385328920860218
1105	84-85	0.19444916572154725
1105	86-87	0.180692258284477
1105	88-89	0.14424254842819906
1105	90-91	0.16364118249631687
1105	92-93	0.09383871300358138
1105	94-95	0.055310240179704095
1105	96-97	0.046292321006568216
1105	98-99	0.0775845190852138
1105	100	0.06650953943463023
1106	1	0.0750733057676598
1106	2	0.09474996750628861
1106	3	0.15626185466980402
1106	4	0.17843319322081896
1106	5	0.17581087822343733
1106	6	0.19505235381237895
1106	7	0.16883310089760073
1106	8	0.21618023738300707
1106	9	0.2676349180296498
1106	10-11	0.2525324300200751
1106	12-13	0.27755062727699453
1106	14-15	0.24694543115188594
1106	16-17	0.3071418678253508
1106	18-19	0.3123590857895522
1106	20-21	0.29050127959180116
1106	22-23	0.3386302506558394
1106	24-25	0.3099624144853834
1106	26-27	0.29587460172302116
1106	28-29	0.3210133089432077
1106	30-31	0.29913236113443276
1106	32-33	0.24284002585334008
1106	34-35	0.31023607743015447
1106	36-37	0.2822095191203786
1106	38-39	0.28089850017357065
1106	40-41	0.3137419997981823
1106	42-43	0.3114593737653095
1106	44-45	0.2700024380107777
1106	46-47	0.22155948701511363
1106	48-49	0.16791982820471674
1106	50-51	0.24946600601933966
1106	52-53	0.2646330466441711
1106	54-55	0.3274564605343926
1106	56-57	0.20477949425291797
1106	58-59	0.27562064749313464
1106	60-61	0.31192485890566957
1106	62-63	0.24505588094931596
1106	64-65	0.2610965027547536
1106	66-67	0.32958303091530894
1106	68-69	0.36455346829190916
1106	70-71	0.294717342496277
1106	72-73	0.25109437832923476
1106	74-75	0.27488768597496005
1106	76-77	0.3602098785600347
1106	78-79	0.2518686695005812
1106	80-81	0.35402843305136145
1106	82-83	0.3093978960812933
1106	84-85	0.32363445375960964
1106	86-87	0.2755476200943292
1106	88-89	0.20455209061276136
1106	90-91	0.21942532531670622
1106	92-93	0.22434918757492284
1106	94-95	0.3584459539594391
1106	96-97	0.25928445915278076
1106	98-99	0.2882509364904777
1106	100	0.2893937069148578
1107	1	-0.012883589536361484
1107	2	-0.008546586472988338
1107	3	-0.027790278538706303
1107	4	0.04585347981874577
1107	5	6.743062647274201E-4
1107	6	0.09637773666872107
1107	7	0.06901774435157648
1107	8	0.060227304386671676
1107	9	0.1769137470384905
1107	10-11	0.06193947955028989
1107	12-13	0.0852181058787096
1107	14-15	0.10658973634655666
1107	16-17	0.21967860070932943
1107	18-19	0.18362770100897308
1107	20-21	0.11079647931528314
1107	22-23	0.08066167757045406
1107	24-25	0.1341527467202681
1107	26-27	0.1558339371541635
1107	28-29	0.1996112920961295
1107	30-31	0.1407789103858761
1107	32-33	0.22403685297125975
1107	34-35	0.19281397243785392
1107	36-37	0.2889467229737761
1107	38-39	0.2861199010203137
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2216	38-39	-0.23624759070423806
2216	40-41	-0.6232580734866673
2216	42-43	-0.45763054016936167
2216	44-45	-0.17218978452802247
2216	46-47	-0.06357542118657022
2216	48-49	-0.25085193747700885
2216	50-51	-0.5228389272996594
2216	52-53	-0.7969557031130492
2216	54-55	-0.1609090606210941
2216	56-57	-0.1206306133377808
2216	58-59	-1.0288397049116824
2216	60-61	-1.6651207495738163
2216	62-63	-0.8234768216742481
2216	64-65	-0.8647626470555849
2216	66-67	-0.5123811377239704
2216	68-69	-0.8269026687553591
2216	70-71	-0.6136607915400702
2216	72-73	-1.0790566837588145
2216	74-75	-1.4081087178094336
2216	76-77	-2.1635117691593777
2216	78-79	-1.1999920754875433
2216	80-81	-1.1244761025576757
2216	82-83	-1.2782855501862898
2216	84-85	-1.2637714959840736
2216	86-87	-0.9386680463656276
2216	88-89	-0.9449252536889823
2216	90-91	-1.1070117888903326
2216	92-93	-1.110538885431243
2216	94-95	-0.9778903327860924
2216	96-97	-1.1568991683551317
2216	98-99	-0.8827480805724051
2216	100	-0.4979491126982687
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62852.0
3	11179.0
4	7372.0
5	5930.0
6	5296.0
7	5466.0
8	5966.0
9	6790.0
10	7723.0
11	8514.0
12	9546.0
13	10652.0
14	11849.0
15	12541.0
16	12630.0
17	13219.0
18	12496.0
19	11677.0
20	11533.0
21	12460.0
22	13911.0
23	16574.0
24	20226.0
25	24874.0
26	30815.0
27	36627.0
28	44433.0
29	55290.0
30	67397.0
31	80746.0
32	104075.0
33	138051.0
34	191600.0
35	280532.0
36	459601.0
37	780545.0
38	964092.0
39	312412.0
40	652.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.33300360563188	20.255476056649734	14.091076203294966	23.32044413442342
2	15.391370592241943	20.20119976147513	40.39412459252432	24.0133050537586
3	18.345914786085995	25.10718715057506	30.28131390500503	26.26558415833391
4	12.49816717959778	16.62372630295752	38.9183993446067	31.959707172838
5	13.096104422718158	37.91819696304376	35.559174304571776	13.426524309666302
6	31.120070011487556	37.78856592825995	17.628738432924017	13.462625627328478
7	27.14510516863056	32.41738505282676	22.518254357189313	17.919255421353363
8	24.810242201575782	36.575781704376865	20.511841129710263	18.102134964337093
9	24.78626820234096	16.96078413625045	21.54535996600876	36.70758769539983
10-11	23.869713331676525	27.107549174746627	28.940852207972696	20.081885285604155
12-13	24.11079490389888	24.540784416002893	29.091892511156075	22.256528168942147
14-15	21.545721803021863	26.776532848395323	26.145236761569908	25.53250858701291
16-17	21.294234686014025	30.10165793118572	26.81760326124622	21.78650412155404
18-19	21.202101060421843	29.162137311640357	28.696066841061114	20.939694786876686
20-21	22.23163825736515	28.35332250992132	28.298485670530027	21.1165535621835
22-23	21.89733142068469	28.164320206760525	28.337857642151093	21.600490730403692
24-25	21.76334382128469	28.337548943270157	28.249043115591327	21.65006411985383
26-27	21.528920015066003	28.284209569497886	28.331740145325124	21.855130270110987
28-29	21.54294686994759	28.30564235922548	28.50278668226547	21.648624088561466
30-31	21.779682976102183	28.62326879691665	28.058368270301976	21.538679956679193
32-33	21.52130558101334	28.39333038183921	28.463967118424566	21.621396918722883
34-35	21.698432920551113	28.18526802829243	28.44311845668334	21.673180594473116
36-37	21.865096615015737	28.49507466804791	28.018483861983647	21.621344854952703
38-39	21.740272754013894	28.61319550172451	28.113342578136365	21.533189166125233
40-41	21.664370094139002	28.327994777339356	28.299755792256754	21.707879336264895
42-43	21.601990171974286	28.361295344391422	28.260623053644263	21.776091429990025
44-45	21.724423099400987	28.402495549572233	28.15923480570689	21.713846545319885
46-47	21.717209591950095	28.501004376433254	28.06018035332122	21.721605678295436
48-49	21.70291659140503	28.375870540594672	28.056338844014928	21.86487402398537
50-51	21.73331538673687	28.329943779829915	28.009025534722593	21.927715298710627
52-53	21.675567798697575	28.288041106615108	28.129740814464892	21.90665028022242
54-55	21.790753991132497	28.577143534663385	27.943002421877296	21.689100052326822
56-57	21.696507130356977	28.396217571728634	27.975901791850077	21.93137350606431
58-59	21.621081719318987	28.085944227484095	28.2333169384748	22.059657114722118
60-61	21.788823036715826	28.373922539467127	27.9615699601689	21.875684463648142
62-63	21.66686535268406	28.45230746872376	27.956441473952005	21.924385704640176
64-65	21.742402683718577	28.22036726325688	28.074666458417997	21.96256359460655
66-67	21.607554014133456	28.255781539620017	27.97510871094267	22.161555735303853
68-69	21.907859707900204	28.392714132517288	27.869273200119054	21.830152959463454
70-71	21.867517337763566	28.191733031605757	27.969238682209724	21.971510948420953
72-73	21.72988647598991	28.330914027118897	28.008842675191104	21.930356821700084
74-75	21.80015396363262	28.299919811685463	27.96224193184278	21.93768429283914
76-77	21.821806782691784	28.28369155618939	27.86795608362549	22.02654557749334
78-79	21.85782875123057	28.362141660790186	27.90799126272817	21.872038325251072
80-81	21.832743788706242	28.270757302160348	27.922590234720563	21.973908674412847
82-83	21.751444278057498	28.347556098705066	28.01737814109952	21.88362148213791
84-85	21.792962204431205	28.22791896812098	27.930061647944726	22.04905717950309
86-87	21.929236719617997	28.33413216529318	27.834727507363716	21.901903607725107
88-89	21.764372982737278	28.266380111869633	27.90036850702098	22.06887839837211
90-91	21.7340962884293	28.1705731136424	27.94388980387989	22.151440794048412
92-93	21.85068000784562	28.313919037258824	27.831118444725533	22.00428251017002
94-95	21.79289140184028	28.107336871319493	27.939083196548296	22.16068853029193
96-97	21.781802849118336	28.132445431193876	27.938424732895157	22.14732698679263
98-99	21.764094097841237	28.215495596854716	27.865366439305255	22.155043865998785
100	21.848566134042578	28.128813198267693	27.86830066305701	22.154320004632712
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1523.0
1	1197.0
2	1028.5
3	1202.5
4	1097.5
5	894.0
6	763.0
7	712.0
8	668.0
9	639.0
10	573.0
11	508.0
12	545.0
13	600.0
14	693.0
15	810.5
16	934.0
17	1194.5
18	1554.0
19	2066.0
20	3072.5
21	4543.5
22	6604.0
23	9668.0
24	13908.0
25	19649.5
26	27236.0
27	36138.0
28	46521.5
29	58530.5
30	69673.5
31	80153.0
32	89315.5
33	97411.0
34	106103.5
35	113801.5
36	120274.5
37	126908.0
38	132900.0
39	136117.5
40	138502.0
41	140412.5
42	139809.5
43	138140.5
44	135938.5
45	133233.0
46	130597.5
47	126636.0
48	122321.0
49	118771.5
50	115435.5
51	111974.0
52	106244.0
53	100239.0
54	95750.5
55	91130.5
56	84402.0
57	76222.0
58	69806.0
59	64527.5
60	57663.5
61	49283.0
62	42170.5
63	35486.5
64	28748.5
65	23073.0
66	18198.0
67	13997.0
68	10771.5
69	8357.5
70	6284.0
71	4645.5
72	3471.5
73	2548.5
74	1832.0
75	1279.5
76	902.5
77	651.5
78	458.0
79	335.5
80	241.0
81	149.5
82	113.0
83	85.0
84	59.5
85	47.0
86	37.5
87	25.0
88	21.0
89	17.0
90	11.5
91	10.0
92	10.5
93	9.0
94	7.5
95	7.5
96	5.5
97	9.0
98	11.5
99	10.5
100	20.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007910770643492073
2	0.026937983694505688
3	0.027816958210449248
4	0.029807576967144964
5	0.03221183079016707
6	0.03490045872128856
7	0.03254790928155726
8	0.03208256983194007
9	0.03270302243142965
10-11	0.032858135581302045
12-13	0.0337500361930683
14-15	0.032522057089911856
16-17	0.029885133542081162
18-19	0.03204379154447197
20-21	0.0314879694240959
22-23	0.03244450051497566
24-25	0.030169507650180553
26-27	0.029975616212840058
28-29	0.030505586141570736
30-31	0.030505586141570736
32-33	0.030402177374989143
34-35	0.030337546895875648
36-37	0.028308149851711827
38-39	0.028605450055633915
40-41	0.02971709429638607
42-43	0.029510276763222876
44-45	0.02843741080993882
46-47	0.02761014067728606
48-49	0.02788158868956275
50-51	0.028838119780442505
52-53	0.029122493888541892
54-55	0.02850204128905232
56-57	0.027183579515136978
58-59	0.02786866259374005
60-61	0.02753258410234986
62-63	0.027687697252222254
64-65	0.02514125637515046
66-67	0.02605900917856212
68-69	0.026912131502860287
70-71	0.027907440881208143
72-73	0.025554891441476844
74-75	0.025774635070462733
76-77	0.026408013765775007
78-79	0.026705313969697095
80-81	0.026757018352987893
82-83	0.025516113154008743
84-85	0.026472644244888505
86-87	0.02687335321539219
88-89	0.027235283898427772
90-91	0.02779110601880385
92-93	0.026356309382484205
94-95	0.02757136238981796
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3868144.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.45101647417052
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.22147022882552	54.110197099866895
2	12.357109259447284	16.669991604642075
3	3.469740215927944	7.021125133969432
4	1.4159789154466753	3.8203686861148713
5	0.7067494185302797	2.3835483336198164
6	0.44344771654397663	1.7946599540424637
7	0.26966170627795866	1.2732269334825266
8	0.18992397623237173	1.0248452199751739
9	0.1537315356384755	0.9332413508655308
>10	0.7321449835721633	8.645851558854071
>50	0.03190030165649956	1.4343493251424155
>100	0.008026636013427409	0.8088325461206053
>500	7.673725859279694E-5	0.03506744126421294
>1k	3.836862880052697E-5	0.04469481203996176
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	2.585219164539893E-5	0.0
12-13	0.0	0.0	0.0	2.585219164539893E-5	0.0
14-15	0.0	0.0	0.0	3.87782874680984E-5	0.0
16-17	0.0	0.0	0.0	9.048267075889625E-5	0.0
18-19	0.0	2.585219164539893E-5	0.0	1.0340876658159572E-4	0.0
20-21	0.0	2.585219164539893E-5	0.0	1.163348624042952E-4	0.0
22-23	0.0	2.585219164539893E-5	0.0	2.326697248085904E-4	0.0
24-25	0.0	2.585219164539893E-5	0.0	4.653394496171808E-4	0.0
26-27	0.0	3.87782874680984E-5	0.0	7.626396535392685E-4	0.0
28-29	0.0	5.170438329079786E-5	0.0	0.002274992864795106	0.0
30-31	0.0	5.170438329079786E-5	0.0	0.0072386136607117	0.0
32-33	0.0	5.170438329079786E-5	0.0	0.018962582571900116	0.0
34-35	0.0	5.170438329079786E-5	0.0	0.036593777274062184	0.0
36-37	0.0	5.170438329079786E-5	0.0	0.0651733751380507	0.0
38-39	0.0	5.170438329079786E-5	0.0	0.10125010857920491	0.0
40-41	0.0	5.170438329079786E-5	0.0	0.13662883284593336	0.0
42-43	0.0	5.170438329079786E-5	0.0	0.17221437464582498	0.0
44-45	0.0	6.463047911349733E-5	0.0	0.2174040056419823	1.2926095822699465E-5
46-47	0.0	7.75565749361968E-5	0.0	0.27235283898427776	2.585219164539893E-5
48-49	0.0	7.75565749361968E-5	0.0	0.3283357599923891	2.585219164539893E-5
50-51	0.0	7.75565749361968E-5	0.0	0.3841894200422735	2.585219164539893E-5
52-53	0.0	7.75565749361968E-5	0.0	0.44041793687101616	3.87782874680984E-5
54-55	0.0	7.75565749361968E-5	0.0	0.5013515525792214	5.170438329079786E-5
56-57	0.0	7.75565749361968E-5	0.0	0.5749785943853176	5.170438329079786E-5
58-59	0.0	7.75565749361968E-5	0.0	0.652380056171642	5.170438329079786E-5
60-61	0.0	7.75565749361968E-5	0.0	0.7316687279480805	5.170438329079786E-5
62-63	0.0	7.75565749361968E-5	0.0	0.807648319193908	5.170438329079786E-5
64-65	0.0	7.75565749361968E-5	0.0	0.888811274864638	5.170438329079786E-5
66-67	0.0	7.75565749361968E-5	0.0	0.9792034629527753	5.170438329079786E-5
68-69	0.0	1.0340876658159572E-4	0.0	1.082741490492598	5.170438329079786E-5
70-71	0.0	1.0340876658159572E-4	0.0	1.1923935613565577	5.170438329079786E-5
72-73	0.0	1.0340876658159572E-4	0.0	1.2991889650437005	5.170438329079786E-5
74-75	0.0	1.0340876658159572E-4	0.0	1.4101465715857526	5.170438329079786E-5
76-77	0.0	1.0340876658159572E-4	0.0	1.5295707708916733	5.170438329079786E-5
78-79	0.0	1.0340876658159572E-4	0.0	1.660201895275874	5.170438329079786E-5
80-81	0.0	1.2926095822699465E-4	0.0	1.8016004574803834	5.170438329079786E-5
82-83	0.0	1.2926095822699465E-4	0.0	1.94638565679044	5.170438329079786E-5
84-85	0.0	1.2926095822699465E-4	0.0	2.0911450039088515	5.170438329079786E-5
86-87	0.0	1.2926095822699465E-4	0.0	2.2490243382873025	5.170438329079786E-5
88	0.0	1.2926095822699465E-4	0.0	2.373050227706104	5.170438329079786E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3515	0.0	40.652256	1
GTATCAA	6090	0.0	35.889885	1
TCAACGC	6265	0.0	33.461002	4
ATCAACG	6420	0.0	33.01921	3
CAACGCA	6695	0.0	31.522518	5
AACGCAG	7100	0.0	29.855648	6
TATCAAC	7460	0.0	28.415628	2
ACGCAGA	8470	0.0	24.527153	7
GTACATG	10195	0.0	23.882454	1
CGCAGAG	8625	0.0	23.650425	8
TACATGG	10480	0.0	22.424784	2
ACATGGG	10965	0.0	20.747314	3
GCAGAGT	9915	0.0	19.530474	9
TACACCG	490	0.0	19.184877	5
GAGTACA	7800	0.0	18.620882	1
AGAGTAC	8510	0.0	17.369984	10-11
ATACCGT	490	0.0	17.26572	6
TAGACTG	1515	0.0	17.06378	5
GTATAGG	1305	0.0	16.928703	1
CATGGGG	8070	0.0	16.890776	4
>>END_MODULE