FastQCFastQC Report
Wed 25 May 2016
SRR2049356_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049356_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3868144
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93880.24270037516700518No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87050.22504332827319767No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52950.13688735476238734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58900.055.3130871
GTATCAA110050.042.8043251
TCAACGC135400.034.396434
ATCAACG135950.034.2227063
CAACGCA139650.033.416945
TATCAAC141250.033.1382262
AACGCAG144350.032.355666
ACGCAGA167850.027.7139457
CGCAGAG170200.027.0825778
GTGGTAT27700.026.4761491
TGGTATC29000.024.470192
GTACATG101200.024.4351621
GCAGAGT187150.024.1522189
TACATGG103300.022.6107222
GAGTACT131150.021.04328212-13
ACATGGG106150.021.0296483
AGAGTAC170950.020.9106710-11
CAGAGTA181300.020.50819810-11
AGTACTT139800.018.93410912-13
GTACTTT144900.018.81126414-15
Produced by FastQC (version 0.11.5)