##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049356_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3868144
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73416372296378	31.0	31.0	34.0	30.0	34.0
2	32.00383336297718	33.0	31.0	34.0	30.0	34.0
3	31.963546600126573	33.0	31.0	34.0	30.0	34.0
4	35.67546140991649	37.0	35.0	37.0	33.0	37.0
5	35.77663706418375	37.0	35.0	37.0	35.0	37.0
6	35.82958984980911	37.0	35.0	37.0	35.0	37.0
7	35.78445683511266	37.0	35.0	37.0	33.0	37.0
8	35.76708312823928	37.0	35.0	37.0	33.0	37.0
9	37.4946124032611	39.0	37.0	39.0	35.0	39.0
10-11	37.45510366211806	39.0	37.0	39.0	34.5	39.0
12-13	37.4069994550358	39.0	37.0	39.0	34.0	39.0
14-15	38.69791067757561	40.0	38.0	41.0	34.0	41.0
16-17	38.72098867053553	40.0	38.0	41.0	34.5	41.0
18-19	38.788752306015496	40.0	38.0	41.0	35.0	41.0
20-21	38.8190659396341	40.0	38.0	41.0	35.0	41.0
22-23	38.783200160076774	40.0	38.0	41.0	34.5	41.0
24-25	38.762571662275235	40.0	38.0	41.0	34.0	41.0
26-27	38.65425007445431	40.0	38.0	41.0	34.0	41.0
28-29	38.572020328095334	40.0	38.0	41.0	34.0	41.0
30-31	38.47393607890503	40.0	38.0	41.0	34.0	41.0
32-33	38.34136526458167	40.0	38.0	41.0	34.0	41.0
34-35	38.24558612605942	40.0	38.0	41.0	34.0	41.0
36-37	38.137726775425115	40.0	38.0	41.0	34.0	41.0
38-39	38.04653226456926	40.0	38.0	41.0	33.0	41.0
40-41	37.941129518446054	40.0	37.5	41.0	33.0	41.0
42-43	37.80478958384176	40.0	37.0	41.0	33.0	41.0
44-45	37.63700356036384	40.0	37.0	41.0	33.0	41.0
46-47	37.4166739914543	40.0	37.0	41.0	32.5	41.0
48-49	37.223223075459444	40.0	37.0	41.0	32.0	41.0
50-51	37.08145456839249	40.0	37.0	41.0	31.0	41.0
52-53	36.945659468727115	40.0	36.0	41.0	31.0	41.0
54-55	36.80873992798614	39.5	36.0	41.0	31.0	41.0
56-57	36.662684610500534	39.0	35.5	41.0	31.0	41.0
58-59	36.47374335081631	39.0	35.0	41.0	31.0	41.0
60-61	36.29331237409983	39.0	35.0	41.0	30.5	41.0
62-63	36.09267493661042	39.0	35.0	41.0	30.0	41.0
64-65	35.86252851496739	38.0	35.0	40.0	30.0	41.0
66-67	35.601701100062456	37.5	35.0	40.0	30.0	41.0
68-69	35.313386988695356	37.0	35.0	40.0	29.0	41.0
70-71	34.985625147357496	37.0	34.0	39.5	29.0	41.0
72-73	34.69306610612222	36.0	34.0	39.0	29.0	41.0
74-75	34.38422962020029	36.0	34.0	39.0	29.0	40.5
76-77	34.059120860030035	35.0	34.0	38.5	29.0	40.0
78-79	33.72497766370642	35.0	34.0	37.0	29.0	39.0
80-81	33.41129621854822	35.0	34.0	37.0	28.0	39.0
82-83	33.11533502889241	35.0	34.0	36.5	28.0	39.0
84-85	32.848027374368684	35.0	33.0	36.0	28.0	37.0
86-87	32.61165910576235	35.0	33.0	36.0	27.0	37.0
88-89	32.3868238876319	35.0	33.0	35.0	27.0	37.0
90-91	32.19375118920081	35.0	33.0	35.0	27.0	36.0
92-93	32.01799338390711	35.0	33.0	35.0	26.0	36.0
94-95	31.85964341037976	35.0	33.0	35.0	26.0	36.0
96-97	31.66979409246398	35.0	33.0	35.0	26.0	36.0
98-99	31.51933058851997	35.0	33.0	35.0	25.0	35.0
100	31.402967417965826	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22658914299592325
1101	2	0.23993486569432676
1101	3	0.430666988967765
1101	4	0.3027628139853533
1101	5	0.23811285167128204
1101	6	0.23167478065744262
1101	7	0.15413795636317928
1101	8	0.1710680906611799
1101	9	0.2268812351953997
1101	10-11	0.28498348174959176
1101	12-13	0.2667021979185833
1101	14-15	0.40590080705526077
1101	16-17	0.31715627913148836
1101	18-19	0.42333577790464005
1101	20-21	0.3939075869739881
1101	22-23	0.43193547987882397
1101	24-25	0.3942530864428804
1101	26-27	0.4886264910078921
1101	28-29	0.5198678036122502
1101	30-31	0.4980994383835693
1101	32-33	0.5145990446790023
1101	34-35	0.5411380934164285
1101	36-37	0.5531936983839998
1101	38-39	0.4787476363071477
1101	40-41	0.5249634698194683
1101	42-43	0.4938175394112392
1101	44-45	0.5463246479036385
1101	46-47	0.5759159026353089
1101	48-49	0.6355550746000631
1101	50-51	0.5361819670268062
1101	52-53	0.637715641128203
1101	54-55	0.5865610903412843
1101	56-57	0.5590976283614495
1101	58-59	0.5602493349549249
1101	60-61	0.5825149002745533
1101	62-63	0.5563972261213763
1101	64-65	0.5000028164111328
1101	66-67	0.516918966993174
1101	68-69	0.566621599426874
1101	70-71	0.52717491671131
1101	72-73	0.5022093493393527
1101	74-75	0.5995839178313602
1101	76-77	0.5461361793913042
1101	78-79	0.5681552664138536
1101	80-81	0.5843122778095733
1101	82-83	0.5174099503960292
1101	84-85	0.537036667926678
1101	86-87	0.5466171540099083
1101	88-89	0.5779361771354488
1101	90-91	0.6292275810037182
1101	92-93	0.6846641210092841
1101	94-95	0.7127673016716578
1101	96-97	0.7730088407658222
1101	98-99	0.7646125300304334
1101	100	0.7794482666215679
1102	1	0.22316441705322987
1102	2	0.26506730201072415
1102	3	0.41056959467137233
1102	4	0.2342239123979013
1102	5	0.15129293889336992
1102	6	0.1738385536730931
1102	7	0.21318198870999083
1102	8	0.2029368970006118
1102	9	0.1912566235063693
1102	10-11	0.2638293564230736
1102	12-13	0.25930825307132466
1102	14-15	0.37408521749561174
1102	16-17	0.2959281572843011
1102	18-19	0.2781185699598723
1102	20-21	0.23062141530412106
1102	22-23	0.2878340332142173
1102	24-25	0.260638259719812
1102	26-27	0.3461750680919806
1102	28-29	0.30522918501178253
1102	30-31	0.27216104258926777
1102	32-33	0.2577180057660371
1102	34-35	0.27540911976684157
1102	36-37	0.2682117256061005
1102	38-39	0.33966116532401713
1102	40-41	0.3311941923976889
1102	42-43	0.31336288475232266
1102	44-45	0.3119043231373837
1102	46-47	0.3113755028422034
1102	48-49	0.29661666040909296
1102	50-51	0.3720600672685208
1102	52-53	0.5120865948087214
1102	54-55	0.3855689632068007
1102	56-57	0.4370573449895332
1102	58-59	0.43655590100696173
1102	60-61	0.40877072065112685
1102	62-63	0.4532076284869788
1102	64-65	0.3157317020238324
1102	66-67	0.3048832196459301
1102	68-69	0.39624606017817854
1102	70-71	0.3374496895472987
1102	72-73	0.29751898994303616
1102	74-75	0.3097662610441958
1102	76-77	0.37897954191706873
1102	78-79	0.33863845959550787
1102	80-81	0.32189645093890107
1102	82-83	0.37003667357387116
1102	84-85	0.42292005341730743
1102	86-87	0.42461096216320016
1102	88-89	0.39990824406363856
1102	90-91	0.3926216191093488
1102	92-93	0.44644735731686325
1102	94-95	0.4605280531156559
1102	96-97	0.5192828617060847
1102	98-99	0.5464492788003028
1102	100	0.6220656663896023
1103	1	0.22296878780508322
1103	2	0.22336207513437856
1103	3	0.38718171356966735
1103	4	0.2544564820931825
1103	5	0.20768709976479727
1103	6	0.1759493477929155
1103	7	0.12902825600739476
1103	8	0.1475404866290262
1103	9	0.2840606469616844
1103	10-11	0.22182662605760584
1103	12-13	0.2341171491888545
1103	14-15	0.32380974074970226
1103	16-17	0.2778304736899031
1103	18-19	0.26975587723103445
1103	20-21	0.315911707880268
1103	22-23	0.3229880865232104
1103	24-25	0.2890054159605313
1103	26-27	0.3021397096849441
1103	28-29	0.38699329434319907
1103	30-31	0.316710186111969
1103	32-33	0.2683905467032872
1103	34-35	0.28745103591665355
1103	36-37	0.31642581746049103
1103	38-39	0.3083756907307418
1103	40-41	0.3254744578972293
1103	42-43	0.3113750896436329
1103	44-45	0.38900868608591566
1103	46-47	0.3385450573333557
1103	48-49	0.29909227384747794
1103	50-51	0.26806179043565237
1103	52-53	0.22664895142489883
1103	54-55	0.23563868971054802
1103	56-57	0.28007377498250463
1103	58-59	0.3331831151847595
1103	60-61	0.22636515051424766
1103	62-63	0.17353766736984255
1103	64-65	0.193835917615651
1103	66-67	0.18214551497812437
1103	68-69	0.15454429357166788
1103	70-71	0.17123852173900644
1103	72-73	0.20349437992533126
1103	74-75	0.1653631928713608
1103	76-77	0.13217588497872867
1103	78-79	0.16816293732873788
1103	80-81	0.14239663648553602
1103	82-83	0.12632285749293715
1103	84-85	0.17574818184445462
1103	86-87	0.1540406101979528
1103	88-89	0.1646414869319841
1103	90-91	0.19094866426833335
1103	92-93	0.23298960031750227
1103	94-95	0.18371783376968764
1103	96-97	0.27755902554585177
1103	98-99	0.33244198253202484
1103	100	0.33254252788707817
1104	1	0.220419935800102
1104	2	0.1708778377419513
1104	3	0.33134842592916414
1104	4	0.1795246423272232
1104	5	0.16879036398933067
1104	6	0.18922727166965814
1104	7	0.2288544249183886
1104	8	0.17122787346844603
1104	9	0.26448569932424704
1104	10-11	0.25538227446286754
1104	12-13	0.22742045135614575
1104	14-15	0.26497709110853407
1104	16-17	0.25952564488638785
1104	18-19	0.24245158809874567
1104	20-21	0.31325057195807204
1104	22-23	0.2985540718496509
1104	24-25	0.31716907023034224
1104	26-27	0.33532450237624545
1104	28-29	0.3540730808153185
1104	30-31	0.3607542716823744
1104	32-33	0.30692658544853657
1104	34-35	0.33286526864345234
1104	36-37	0.35810409152137623
1104	38-39	0.33368398978028324
1104	40-41	0.326300470815049
1104	42-43	0.3879595503145765
1104	44-45	0.42656599161760766
1104	46-47	0.360012268800169
1104	48-49	0.277923872199473
1104	50-51	0.2869939923438096
1104	52-53	0.3309607530242502
1104	54-55	0.25425699254162026
1104	56-57	0.19792738537445587
1104	58-59	0.31970418538579537
1104	60-61	0.26863350666778985
1104	62-63	0.30546116808909574
1104	64-65	0.20139283068494507
1104	66-67	0.22231355972621003
1104	68-69	0.20897566868764983
1104	70-71	0.30968586681589017
1104	72-73	0.3063896189597273
1104	74-75	0.3523344897017253
1104	76-77	0.26511475818092123
1104	78-79	0.27122802615487984
1104	80-81	0.2602485325110564
1104	82-83	0.31814107911121425
1104	84-85	0.32195069725923275
1104	86-87	0.3222152250782955
1104	88-89	0.2895033422930027
1104	90-91	0.27404910588062137
1104	92-93	0.34172633753247084
1104	94-95	0.36956746739824453
1104	96-97	0.42614003311442517
1104	98-99	0.5390271332000438
1104	100	0.48499521243614296
1105	1	0.18594987122426687
1105	2	0.18637358114207458
1105	3	0.28068157908895586
1105	4	0.2327012246808593
1105	5	0.22412083911514458
1105	6	0.1777248290897404
1105	7	0.17644685599177734
1105	8	0.17821655938911363
1105	9	0.21240489968131726
1105	10-11	0.1949113253987491
1105	12-13	0.2076314591404511
1105	14-15	0.27133516963963444
1105	16-17	0.24976986192998396
1105	18-19	0.2299121239512374
1105	20-21	0.25115335195389576
1105	22-23	0.24379319080103556
1105	24-25	0.26344254909475495
1105	26-27	0.2702384490366825
1105	28-29	0.3133137312417986
1105	30-31	0.2876664657456516
1105	32-33	0.2886864714478179
1105	34-35	0.3188113230476759
1105	36-37	0.3327401614450949
1105	38-39	0.314288308212447
1105	40-41	0.3167228139719356
1105	42-43	0.3605365233172222
1105	44-45	0.31226103152195606
1105	46-47	0.2740052010835825
1105	48-49	0.3215891343314965
1105	50-51	0.3130925390804151
1105	52-53	0.27336500511979267
1105	54-55	0.24548376960553497
1105	56-57	0.270494325986796
1105	58-59	0.3136202572750406
1105	60-61	0.29961987490155906
1105	62-63	0.3449728158989629
1105	64-65	0.30835121469208815
1105	66-67	0.33960202984810905
1105	68-69	0.36684597649776407
1105	70-71	0.33548446555713696
1105	72-73	0.41207347991488774
1105	74-75	0.38078614381766585
1105	76-77	0.3174013241961049
1105	78-79	0.3182920164255094
1105	80-81	0.36083409651566
1105	82-83	0.3181885794080941
1105	84-85	0.32958534626889247
1105	86-87	0.2882961229976928
1105	88-89	0.3270048266772818
1105	90-91	0.3105569741410079
1105	92-93	0.3746745499243147
1105	94-95	0.31796535609194976
1105	96-97	0.40004148637781256
1105	98-99	0.49337811944542764
1105	100	0.5110003965202701
1106	1	0.2013166236943782
1106	2	0.1878369266668436
1106	3	0.29560461430458673
1106	4	0.19944820891081605
1106	5	0.1348934056607476
1106	6	0.18385905635542343
1106	7	0.18809439397617922
1106	8	0.14514878532007458
1106	9	0.21516797298920665
1106	10-11	0.17173976165094018
1106	12-13	0.17801238702136146
1106	14-15	0.2652199831755908
1106	16-17	0.2216095016300912
1106	18-19	0.22611216581437077
1106	20-21	0.22900474528785963
1106	22-23	0.2427554804268155
1106	24-25	0.2544133743257646
1106	26-27	0.2945006645764323
1106	28-29	0.2940095274056205
1106	30-31	0.3623732240851254
1106	32-33	0.32196188346146926
1106	34-35	0.264548979681571
1106	36-37	0.31432981006640404
1106	38-39	0.4219744540186525
1106	40-41	0.357129711908442
1106	42-43	0.3760913669509591
1106	44-45	0.36546057859055026
1106	46-47	0.36044639270222234
1106	48-49	0.3366916058009224
1106	50-51	0.25018452665782576
1106	52-53	0.28761450193664473
1106	54-55	0.3534455143714368
1106	56-57	0.33927095314979994
1106	58-59	0.3970050407185468
1106	60-61	0.30372541793190067
1106	62-63	0.27584394722084227
1106	64-65	0.2006973184114642
1106	66-67	0.17954310722194577
1106	68-69	0.2827457116308949
1106	70-71	0.32581546659199745
1106	72-73	0.3624634035759229
1106	74-75	0.29367918990385533
1106	76-77	0.257257050843684
1106	78-79	0.3115730900817226
1106	80-81	0.29389752981452943
1106	82-83	0.39747437092223237
1106	84-85	0.38460881572132166
1106	86-87	0.39462161045553046
1106	88-89	0.40061587343137717
1106	90-91	0.35266143277772244
1106	92-93	0.44551884836264577
1106	94-95	0.4038343383331373
1106	96-97	0.4339355513466323
1106	98-99	0.42979505068603174
1106	100	0.5084942009363509
1107	1	0.12918766548466465
1107	2	0.10630043929245758
1107	3	0.24569575012220213
1107	4	0.19154177747656576
1107	5	0.1579352031156489
1107	6	0.18807477970057818
1107	7	0.09653391539164602
1107	8	0.12561633088086666
1107	9	0.13024690026512786
1107	10-11	0.13858070780212017
1107	12-13	0.1573509468593386
1107	14-15	0.19044383806413379
1107	16-17	0.15281376571745398
1107	18-19	0.1451115777087324
1107	20-21	0.10459405000869282
1107	22-23	0.15121004971454255
1107	24-25	0.18835845944823149
1107	26-27	0.17972490317254142
1107	28-29	0.265997689183898
1107	30-31	0.21396831435706787
1107	32-33	0.2444860288398445
1107	34-35	0.27265986189167535
1107	36-37	0.1918451370226748
1107	38-39	0.19755109322738917
1107	40-41	0.17641512297783635
1107	42-43	0.21535990437472208
1107	44-45	0.13394700825595152
1107	46-47	0.2679196615143482
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2216	88-89	-1.5953862574159245
2216	90-91	-1.4030317482151418
2216	92-93	-1.552990265084599
2216	94-95	-1.4957823293358565
2216	96-97	-1.5093740186356364
2216	98-99	-1.676852114981429
2216	100	-1.67908799393496
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	164.0
3	19.0
4	38.0
5	71.0
6	156.0
7	514.0
8	1256.0
9	2078.0
10	3012.0
11	4135.0
12	5789.0
13	8098.0
14	9856.0
15	12529.0
16	15412.0
17	20692.0
18	20366.0
19	11675.0
20	9344.0
21	9511.0
22	9881.0
23	10803.0
24	13129.0
25	16366.0
26	21248.0
27	27704.0
28	36295.0
29	47669.0
30	61343.0
31	79775.0
32	103320.0
33	133981.0
34	183129.0
35	265717.0
36	432501.0
37	782278.0
38	1080946.0
39	425911.0
40	1433.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.52390513719098	20.534643576552273	14.66125542217095	23.2801958640858
2	15.556013426594253	20.6769448086731	39.604549365276995	24.162492399455658
3	18.07867028735228	25.262761675883834	30.77380262989175	25.884765406872134
4	12.567477469162853	17.23865948078962	38.23359167435123	31.960271375696298
5	12.715452165172755	37.79649878598108	35.67188811998726	13.816160928858906
6	30.769226791970517	38.19987053222424	17.14972348495816	13.881179190847085
7	26.56418685550486	32.94719638152044	22.36744547255738	18.12117129041732
8	24.9854645789433	36.295017472235344	20.19253713092375	18.52698081789761
9	24.7342394698853	16.391504556190256	21.572413022886426	37.30184295103802
10-11	23.850805450882902	27.028634404510278	28.66231195115797	20.458248193448846
12-13	24.39594891425595	24.45106308801276	29.18582580351991	21.967162194211383
14-15	21.59303396644538	26.42960791760176	26.7835505479247	25.193807568028152
16-17	20.74854513541186	29.815737187619852	27.457939448213338	21.977778228754957
18-19	20.63769359095566	28.24682282945589	30.276944259816663	20.838539319771787
20-21	21.660417756229673	27.36707374420943	30.1830388292464	20.7894696703145
22-23	21.286112926509457	27.235917277123082	30.27397635661961	21.203993439747848
24-25	21.25195555849122	27.368731823469183	30.127783621096494	21.251528996943104
26-27	21.003451141446387	27.381554522127622	30.206775064142576	21.408219272283414
28-29	21.007168206280955	27.381462195160573	30.378096867407894	21.233272731150578
30-31	21.26969017800672	27.617688654277167	29.97970213336356	21.13291903435255
32-33	21.02606772020584	27.45615362849786	30.356620041717765	21.16115860957854
34-35	21.208274718867074	27.282260857178642	30.28207200689426	21.227392417060017
36-37	21.396637479946758	27.58775178926412	29.81027964211745	21.205331088671674
38-39	21.271326547099694	27.706097915656812	29.868786556794312	21.153788980449185
40-41	21.23466042663135	27.477530870453126	29.950527908694113	21.337280794221407
42-43	21.236219549286986	27.516435328779608	29.81080838977016	21.436536732163248
44-45	21.367958867958865	27.614960001323634	29.577227020408834	21.439854110308655
46-47	21.445181491762057	27.768354636870413	29.26982679258775	21.516637078779784
48-49	21.508968862013827	27.766903949091347	28.975997290662995	21.74812989823182
50-51	21.559989979630938	27.830644387804824	28.831078448683485	21.77828718388075
52-53	21.548408345772643	27.866034972082005	28.872352262627217	21.713204419518142
54-55	21.572847716360695	28.178879477493485	28.674080768868837	21.574192037276983
56-57	21.542980133623104	27.913273598003784	28.731772307389235	21.811973960983874
58-59	21.4555380866213	27.65924883984833	29.00891682597033	21.876296247560035
60-61	21.548203662514837	27.92130159900076	28.787390924488278	21.743103813996125
62-63	21.38776322711826	27.974041485537455	28.8098017241045	21.828393563239786
64-65	21.395148992748293	27.7918795012932	28.947853152089596	21.86511835386891
66-67	21.325981519726696	27.83550610052221	28.796708850058444	22.041803529692647
68-69	21.6684082732604	27.87144047841873	28.682541397842236	21.777609850478637
70-71	21.663202668271925	27.76966804170965	28.681428440135605	21.885700849882816
72-73	21.494301577420163	27.935300334428792	28.700510433061506	21.869887655089542
74-75	21.63954650533312	27.87337898199992	28.57152644170488	21.915548070962082
76-77	21.637061111393333	27.88950982114385	28.466018985765263	22.007410081697554
78-79	21.6677031636404	27.98081642524754	28.490201581102774	21.861278830009283
80-81	21.586196065048657	27.85735106588565	28.516601808027474	22.039851061038217
82-83	21.522467947988773	27.917125980587826	28.584057549879944	21.97634852154346
84-85	21.5404468546391	27.81943136710467	28.53483725756939	22.105284520686844
86-87	21.65245984197902	27.903923741253443	28.424619463746225	22.018996953021308
88-89	21.469618298823285	27.822273330348196	28.474513786267607	22.23359458456091
90-91	21.373925515877765	27.752035344190396	28.58186580779464	22.2921733321372
92-93	21.48615340227806	27.90870588770603	28.358470186084585	22.24667052393133
94-95	21.38516094914227	27.718472436427678	28.509376931323132	22.38698968310692
96-97	21.381238094647166	27.754130808416118	28.533020097166293	22.331610999770422
98-99	21.359940786150204	27.80575509809427	28.441817945850246	22.39248616990528
100	21.424279902628896	27.688098099112533	28.443535927827906	22.444086070430664
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	582.0
1	434.5
2	493.5
3	724.0
4	485.0
5	308.0
6	832.5
7	2174.5
8	3599.5
9	4078.0
10	3131.5
11	1951.0
12	1750.5
13	1980.0
14	2288.5
15	2622.5
16	2989.0
17	3474.5
18	4023.5
19	4715.0
20	5760.0
21	7164.0
22	9125.0
23	11934.0
24	15920.0
25	21385.0
26	28494.0
27	37078.0
28	47320.0
29	59046.5
30	70176.5
31	80402.5
32	89173.5
33	96960.0
34	104617.0
35	111661.0
36	118489.5
37	125725.5
38	131811.0
39	134817.5
40	137583.5
41	139173.5
42	138819.0
43	138617.0
44	136138.5
45	132326.5
46	129896.5
47	125886.0
48	121193.5
49	117809.5
50	114821.0
51	111418.0
52	105334.5
53	99426.5
54	94917.0
55	89946.0
56	83112.5
57	74969.5
58	68465.0
59	63405.0
60	56533.0
61	48069.0
62	41176.0
63	34579.0
64	27704.0
65	21942.0
66	17057.5
67	12894.0
68	9782.0
69	7383.0
70	5386.0
71	3850.5
72	2862.5
73	2078.5
74	1437.5
75	969.0
76	632.5
77	414.5
78	285.0
79	198.5
80	119.0
81	48.5
82	22.0
83	16.0
84	15.0
85	10.5
86	4.5
87	3.5
88	2.5
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03570187666229593
2	0.0
3	0.0
4	2.585219164539893E-5
5	0.0
6	0.0
7	0.0
8	1.809653415177925E-4
9	0.0
10-11	0.0
12-13	0.04200981142377326
14-15	7.755657493619678E-5
16-17	1.2926095822699465E-5
18-19	2.585219164539893E-5
20-21	7.755657493619678E-5
22-23	0.0
24-25	9.048267075889625E-5
26-27	9.048267075889625E-5
28-29	5.55822120376077E-4
30-31	9.048267075889625E-5
32-33	1.9389143734049196E-4
34-35	1.2926095822699465E-5
36-37	9.048267075889625E-5
38-39	4.911916412625797E-4
40-41	9.048267075889625E-5
42-43	7.367874618938695E-4
44-45	4.136350663263829E-4
46-47	1.809653415177925E-4
48-49	0.0010082354741705582
50-51	5.946004078441753E-4
52-53	7.755657493619679E-4
54-55	5.170438329079786E-4
56-57	6.592308869576727E-4
58-59	1.9389143734049196E-4
60-61	2.4559582063128985E-4
62-63	0.0012667573906245478
64-65	5.041177370852791E-4
66-67	0.0019001360859368214
68-69	0.0017579490318871272
70-71	5.81674312021476E-4
72-73	0.00144772273214234
74-75	0.0012926095822699466
76-77	9.17752803411662E-4
78-79	0.003813198267696342
80-81	0.0030117803266889753
82-83	0.00144772273214234
84-85	0.003360784913901861
86-87	0.0012926095822699466
88-89	0.0023784016313767014
90-91	0.003231523955674866
92-93	0.003050558614157074
94-95	0.004330242100604321
96-97	0.0035158980637742545
98-99	0.003360784913901861
100	0.00144772273214234
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3868144.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.77210587511622
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.77616470107367	51.02498080453538
2	13.087457327644337	16.95404343324489
3	3.7808585323178634	7.346825074623875
4	1.5372942672119538	3.982951481482475
5	0.7948729413944515	2.5742797158633235
6	0.46492222018382	1.80683947616643
7	0.3253565462685155	1.4751820063446508
8	0.22424975153366544	1.162010291904567
9	0.15794959430669728	0.9207655060828546
>10	0.8022987112658447	9.02533311933528
>50	0.036618113075021684	1.6137232266074133
>100	0.011237658164423433	1.1640133514859758
>500	5.197368013997562E-4	0.2394319031005504
>1k	7.995950334459783E-5	0.10397134423338686
>5k	1.1993925501689674E-4	0.6056492649889046
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9388	0.24270037516700518	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8705	0.22504332827319767	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5295	0.13688735476238734	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	2.585219164539893E-5	0.0
7	0.0	0.0	0.0	2.585219164539893E-5	0.0
8	0.0	0.0	0.0	2.585219164539893E-5	0.0
9	0.0	0.0	0.0	5.170438329079786E-5	0.0
10-11	0.0	0.0	0.0	5.170438329079786E-5	0.0
12-13	0.0	0.0	0.0	5.170438329079786E-5	0.0
14-15	0.0	0.0	0.0	6.463047911349733E-5	0.0
16-17	0.0	0.0	0.0	1.2926095822699465E-4	0.0
18-19	0.0	0.0	0.0	1.551131498723936E-4	0.0
20-21	0.0	0.0	0.0	1.6803924569509305E-4	0.0
22-23	0.0	1.2926095822699465E-5	0.0	2.585219164539893E-4	0.0
24-25	0.0	2.585219164539893E-5	0.0	4.782655454398802E-4	0.0
26-27	0.0	2.585219164539893E-5	0.0	7.884918451846674E-4	0.0
28-29	0.0	2.585219164539893E-5	0.0	0.002274992864795106	0.0
30-31	0.0	2.585219164539893E-5	0.0	0.007186909277420902	0.0
32-33	0.0	2.585219164539893E-5	0.0	0.01902721305101361	0.0
34-35	0.0	2.585219164539893E-5	0.0	0.036800594807225376	0.0
36-37	0.0	2.585219164539893E-5	0.0	0.06565164068349058	0.0
38-39	0.0	2.585219164539893E-5	0.0	0.10209030480768039	0.0
40-41	0.0	2.585219164539893E-5	0.0	0.13767584660757198	0.0
42-43	0.0	2.585219164539893E-5	0.0	0.17339064936569062	0.0
44-45	0.0	2.585219164539893E-5	0.0	0.21878709789501116	0.0
46-47	0.0	2.585219164539893E-5	0.0	0.27429175335768263	0.0
48-49	0.0	2.585219164539893E-5	0.0	0.3308175703903474	0.0
50-51	0.0	2.585219164539893E-5	0.0	0.38702023502744465	0.0
52-53	0.0	2.585219164539893E-5	0.0	0.4432358257603647	0.0
54-55	0.0	2.585219164539893E-5	0.0	0.5045572243432509	0.0
56-57	0.0	2.585219164539893E-5	0.0	0.5785591229282054	0.0
58-59	0.0	2.585219164539893E-5	0.0	0.6564000719725016	0.0
60-61	0.0	2.585219164539893E-5	0.0	0.7363479746358977	0.0
62-63	0.0	2.585219164539893E-5	0.0	0.8130255750561509	0.0
64-65	0.0	2.585219164539893E-5	0.0	0.8943824221642214	0.0
66-67	0.0	2.585219164539893E-5	0.0	0.9847616841565361	0.0
68-69	0.0	5.170438329079786E-5	0.0	1.0885582336128128	0.0
70-71	0.0	5.170438329079786E-5	0.0	1.1989470919386662	0.0
72-73	0.0	5.170438329079786E-5	0.0	1.3062078350754263	0.0
74-75	0.0	5.170438329079786E-5	0.0	1.4176954115462093	0.0
76-77	0.0	5.170438329079786E-5	0.0	1.5377788417390872	0.0
78-79	0.0	5.170438329079786E-5	0.0	1.669276014543409	0.0
80-81	0.0	5.170438329079786E-5	0.0	1.8119284080427203	0.0
82-83	0.0	5.170438329079786E-5	0.0	1.9573728382397344	0.0
84-85	0.0	5.170438329079786E-5	0.0	2.102856046724217	0.0
86-87	0.0	5.170438329079786E-5	0.0	2.2620150645891157	0.0
88	0.0	5.170438329079786E-5	0.0	2.3881737598186623	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5890	0.0	55.313087	1
GTATCAA	11005	0.0	42.804325	1
TCAACGC	13540	0.0	34.39643	4
ATCAACG	13595	0.0	34.222706	3
CAACGCA	13965	0.0	33.41694	5
TATCAAC	14125	0.0	33.138226	2
AACGCAG	14435	0.0	32.35566	6
ACGCAGA	16785	0.0	27.713945	7
CGCAGAG	17020	0.0	27.082577	8
GTGGTAT	2770	0.0	26.476149	1
TGGTATC	2900	0.0	24.47019	2
GTACATG	10120	0.0	24.435162	1
GCAGAGT	18715	0.0	24.152218	9
TACATGG	10330	0.0	22.610722	2
GAGTACT	13115	0.0	21.043282	12-13
ACATGGG	10615	0.0	21.029648	3
AGAGTAC	17095	0.0	20.91067	10-11
CAGAGTA	18130	0.0	20.508198	10-11
AGTACTT	13980	0.0	18.934109	12-13
GTACTTT	14490	0.0	18.811264	14-15
>>END_MODULE