FastQCFastQC Report
Wed 25 May 2016
SRR2049355_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049355_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6064132
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57150.043.5980031
GTATCAA96250.035.411491
TCAACGC99300.032.94874
ATCAACG101650.032.233223
CAACGCA106350.030.9855235
AACGCAG112450.029.2585246
TATCAAC117050.028.6349492
ACGCAGA134450.024.1563367
GTACATG157100.023.9398121
CGCAGAG137950.023.2706898
TACATGG159300.022.8108792
ACATGGG164800.021.6787723
GCAGAGT157000.019.6387779
GTGGTAT36350.019.0116521
CATGGGG116050.017.8637144
GAGTACA121100.017.5857541
CAGAGTA147800.017.36314810-11
AGAGTAC134850.017.02644210-11
TGGTATC35250.016.4030382
GTATAGA29450.015.16511
Produced by FastQC (version 0.11.5)