##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049355_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6064132
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.785790118025137	31.0	31.0	34.0	28.0	34.0
2	30.978010373125123	31.0	31.0	34.0	28.0	34.0
3	31.04256454180087	31.0	31.0	34.0	28.0	34.0
4	34.746208195995735	37.0	35.0	37.0	32.0	37.0
5	34.75988913170096	37.0	35.0	37.0	32.0	37.0
6	34.87657920375084	37.0	35.0	37.0	32.0	37.0
7	34.85026876064043	37.0	35.0	37.0	32.0	37.0
8	34.80583684524018	37.0	35.0	37.0	32.0	37.0
9	36.4389025502743	39.0	37.0	39.0	32.0	39.0
10-11	36.3960180121409	39.0	37.0	39.0	32.0	39.0
12-13	36.33764642656195	39.0	37.0	39.0	32.0	39.0
14-15	37.56262751206603	40.0	38.0	41.0	32.0	41.0
16-17	37.5313100044656	40.0	38.0	41.0	32.0	41.0
18-19	37.56000743387512	40.0	38.0	41.0	32.0	41.0
20-21	37.63272031347603	40.0	38.0	41.0	32.0	41.0
22-23	37.58739461805911	40.0	38.0	41.0	32.0	41.0
24-25	37.594800294584616	40.0	38.0	41.0	32.0	41.0
26-27	37.58223749087256	40.0	38.0	41.0	32.0	41.0
28-29	37.52407698249313	40.0	38.0	41.0	32.0	41.0
30-31	37.46451775785883	40.0	38.0	41.0	32.0	41.0
32-33	37.410858965471064	40.0	38.0	41.0	32.0	41.0
34-35	37.313429852780246	40.0	37.0	41.0	31.0	41.0
36-37	37.24050333996687	40.0	37.0	41.0	31.0	41.0
38-39	37.14108465976664	40.0	37.0	41.0	31.0	41.0
40-41	37.03978681532658	40.0	37.0	41.0	31.0	41.0
42-43	36.907163218082985	40.0	37.0	41.0	30.0	41.0
44-45	36.79695808072779	40.0	37.0	41.0	30.0	41.0
46-47	36.685318030676115	40.0	36.5	41.0	30.0	41.0
48-49	36.561320647373776	40.0	36.0	41.0	30.0	41.0
50-51	36.42820110116337	40.0	36.0	41.0	30.0	41.0
52-53	36.28888948987258	39.5	36.0	41.0	30.0	41.0
54-55	36.15567520627849	39.0	35.0	41.0	29.0	41.0
56-57	36.031998726281024	39.0	35.0	41.0	29.0	41.0
58-59	35.825777126883125	39.0	35.0	41.0	28.5	41.0
60-61	35.61161003751238	39.0	35.0	40.5	28.0	41.0
62-63	35.40993162747777	38.5	35.0	40.0	28.0	41.0
64-65	35.16347566312871	38.0	34.5	40.0	28.0	41.0
66-67	34.902791364040226	37.5	34.0	40.0	28.0	41.0
68-69	34.615486767108635	37.0	34.0	40.0	27.5	41.0
70-71	34.32688066486679	36.5	34.0	39.5	27.0	41.0
72-73	33.9800760438592	36.0	34.0	39.0	26.0	41.0
74-75	33.62811454302116	36.0	34.0	39.0	26.0	40.5
76-77	33.28839675653498	35.0	33.0	38.5	26.0	40.0
78-79	32.961399174688154	35.0	33.0	37.0	26.0	39.0
80-81	32.59408617754363	35.0	33.0	37.0	25.5	39.0
82-83	32.265122609468264	35.0	33.0	36.5	25.0	39.0
84-85	31.962631585196366	35.0	33.0	36.0	25.0	37.5
86-87	31.631388218462263	35.0	32.5	36.0	23.5	37.0
88-89	31.36498817967683	35.0	32.0	35.0	23.0	37.0
90-91	31.143909301446605	35.0	32.0	35.0	21.0	36.0
92-93	30.89407519493309	35.0	32.0	35.0	20.0	36.0
94-95	30.6843330587131	35.0	32.0	35.0	18.5	36.0
96-97	30.422127189843494	35.0	32.0	35.0	11.5	36.0
98-99	30.19758244048777	35.0	32.0	35.0	2.0	35.0
100	30.062396563927038	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.34499510825844126
1101	2	0.3618190363156941
1101	3	0.315751848105009
1101	4	0.4268471166901904
1101	5	0.44935350064175594
1101	6	0.545412790772204
1101	7	0.5320132427339885
1101	8	0.5564710470969843
1101	9	0.7203913876998129
1101	10-11	0.6952553996001711
1101	12-13	0.6741405058934333
1101	14-15	0.8295957166921255
1101	16-17	0.8071057671524571
1101	18-19	0.7948566474171201
1101	20-21	0.7872703356159221
1101	22-23	0.7564483096405468
1101	24-25	0.7884159437237486
1101	26-27	0.7131626801997015
1101	28-29	0.7066717662391753
1101	30-31	0.732970573500829
1101	32-33	0.700681274971906
1101	34-35	0.7363366881834565
1101	36-37	0.7375893563407843
1101	38-39	0.8131192039827226
1101	40-41	0.7689915741272912
1101	42-43	0.7016544803661091
1101	44-45	0.7037665310008379
1101	46-47	0.6338644759478882
1101	48-49	0.6379966653781963
1101	50-51	0.6924577293931549
1101	52-53	0.6550176097289011
1101	54-55	0.677138333206635
1101	56-57	0.6745639205537444
1101	58-59	0.7023471559159375
1101	60-61	0.6669895000676931
1101	62-63	0.6809882069088218
1101	64-65	0.6719740566079722
1101	66-67	0.6937301562013474
1101	68-69	0.720785879618866
1101	70-71	0.7332601517229307
1101	72-73	0.8119458391111181
1101	74-75	0.7491208921023329
1101	76-77	0.7417951464958037
1101	78-79	0.6470107490700272
1101	80-81	0.7049612655572375
1101	82-83	0.7334518278200122
1101	84-85	0.6340664972439178
1101	86-87	0.664005445650723
1101	88-89	0.6530075206815944
1101	90-91	0.6536046117033614
1101	92-93	0.6761660921435251
1101	94-95	0.6659444292925834
1101	96-97	0.6574942115124713
1101	98-99	0.7622561639529124
1101	100	0.758311331045654
1102	1	0.1169939668879465
1102	2	0.25297941749757413
1102	3	0.19551336392424545
1102	4	0.27383381247155825
1102	5	0.17608651799238118
1102	6	0.4100697466721783
1102	7	0.4474807920082995
1102	8	0.4152706550995191
1102	9	0.5541926807602735
1102	10-11	0.5076280830285711
1102	12-13	0.5458216387108905
1102	14-15	0.6107894374065737
1102	16-17	0.5535268225751295
1102	18-19	0.5557222279450755
1102	20-21	0.5274968471249437
1102	22-23	0.5351527979496353
1102	24-25	0.5435691530493969
1102	26-27	0.49955519653646974
1102	28-29	0.5035914434610973
1102	30-31	0.4712809283566415
1102	32-33	0.5075500789791505
1102	34-35	0.49249778275736134
1102	36-37	0.5048684780183592
1102	38-39	0.47708368229481835
1102	40-41	0.4933657657445991
1102	42-43	0.534622934166471
1102	44-45	0.4626144967255641
1102	46-47	0.4767170589086689
1102	48-49	0.4878450016525093
1102	50-51	0.5284507327176868
1102	52-53	0.506686113585161
1102	54-55	0.5307662072412214
1102	56-57	0.4277421114678859
1102	58-59	0.5363952030284906
1102	60-61	0.5146042319414548
1102	62-63	0.5175897747028841
1102	64-65	0.48460089968929765
1102	66-67	0.5127709163871259
1102	68-69	0.5339763712076149
1102	70-71	0.5594730589729835
1102	72-73	0.529058569564512
1102	74-75	0.5274656271053288
1102	76-77	0.5506220296666697
1102	78-79	0.5577379122506372
1102	80-81	0.5182680761520899
1102	82-83	0.49946684039158384
1102	84-85	0.5395783015091773
1102	86-87	0.5900854479024815
1102	88-89	0.6111398712812992
1102	90-91	0.6213061902781085
1102	92-93	0.6165368114136385
1102	94-95	0.6068902459587626
1102	96-97	0.5687362483812706
1102	98-99	0.6525369243253891
1102	100	0.6532078094710734
1103	1	0.051212237642431546
1103	2	0.12063022545368085
1103	3	0.10636603371026965
1103	4	0.12328073571855214
1103	5	0.1353226897717903
1103	6	0.28234114642167896
1103	7	0.3315830854976838
1103	8	0.32943300874566717
1103	9	0.38462543028713725
1103	10-11	0.3975065478986082
1103	12-13	0.34781912657241776
1103	14-15	0.45026017821473374
1103	16-17	0.4365994654172951
1103	18-19	0.37864921526372797
1103	20-21	0.3417798198673552
1103	22-23	0.4009097387889966
1103	24-25	0.466973060258546
1103	26-27	0.42116973435430793
1103	28-29	0.43423588789488576
1103	30-31	0.41431763691672074
1103	32-33	0.4340871776928452
1103	34-35	0.4264497048968323
1103	36-37	0.4370969618976446
1103	38-39	0.4458236111483913
1103	40-41	0.42330156870568914
1103	42-43	0.41749528285013326
1103	44-45	0.32623132387398357
1103	46-47	0.388612739593988
1103	48-49	0.43799061812297424
1103	50-51	0.39051525615784044
1103	52-53	0.4239448803598975
1103	54-55	0.35210759299260275
1103	56-57	0.3470233532012301
1103	58-59	0.4239714361129643
1103	60-61	0.37675653566342504
1103	62-63	0.37122728831749896
1103	64-65	0.34356006890908475
1103	66-67	0.44079513448179597
1103	68-69	0.46068974988313016
1103	70-71	0.3713594080360849
1103	72-73	0.4029170987460091
1103	74-75	0.38419253115193186
1103	76-77	0.4621064817932208
1103	78-79	0.37728687288126395
1103	80-81	0.38644276357187124
1103	82-83	0.37273561378957965
1103	84-85	0.4308044389856214
1103	86-87	0.43238321119759604
1103	88-89	0.4092982598786854
1103	90-91	0.48294229496849894
1103	92-93	0.46912552468639035
1103	94-95	0.4573285204637081
1103	96-97	0.45714102948792856
1103	98-99	0.5070122764759404
1103	100	0.5538388313049474
1104	1	0.02766359088572301
1104	2	0.05466222905824125
1104	3	0.05386715274841691
1104	4	0.15823074785610203
1104	5	0.14293610199799645
1104	6	0.2516960807643258
1104	7	0.30960004812643405
1104	8	0.28548849727894776
1104	9	0.34758430569269905
1104	10-11	0.3519099703923203
1104	12-13	0.32925355323517635
1104	14-15	0.4119789879770721
1104	16-17	0.4664685683226466
1104	18-19	0.421900753699056
1104	20-21	0.4010257736506375
1104	22-23	0.41619651886597353
1104	24-25	0.48371908446390677
1104	26-27	0.43760515940199696
1104	28-29	0.48719370417853014
1104	30-31	0.4261904658563225
1104	32-33	0.45853364203461666
1104	34-35	0.44465304934514194
1104	36-37	0.40572972348680736
1104	38-39	0.4790192104804021
1104	40-41	0.5158498285184763
1104	42-43	0.42718546046940986
1104	44-45	0.40408419811615204
1104	46-47	0.3446579980469906
1104	48-49	0.31298991718519176
1104	50-51	0.3561307744148223
1104	52-53	0.38810480527452285
1104	54-55	0.42092709622699687
1104	56-57	0.4286343899213776
1104	58-59	0.5050604957906444
1104	60-61	0.4573977633991291
1104	62-63	0.4160323478341752
1104	64-65	0.4626711261484786
1104	66-67	0.5116943578821278
1104	68-69	0.47802009683847047
1104	70-71	0.48918130443951213
1104	72-73	0.5155917151524676
1104	74-75	0.5139793403498345
1104	76-77	0.5069718777718606
1104	78-79	0.4616803995123391
1104	80-81	0.5381229295014052
1104	82-83	0.49276473865742787
1104	84-85	0.4878568022228116
1104	86-87	0.5186418495581506
1104	88-89	0.5209221984504033
1104	90-91	0.5676410693352771
1104	92-93	0.5129704274766596
1104	94-95	0.5246988454959443
1104	96-97	0.4998429013759349
1104	98-99	0.4863104614508913
1104	100	0.5221790835125404
1105	1	0.03705018997707654
1105	2	0.08088428088424493
1105	3	0.07793854669239764
1105	4	0.1826664475865769
1105	5	0.15087268478387728
1105	6	0.2468626268096088
1105	7	0.23720649051995224
1105	8	0.3150117169423936
1105	9	0.381713379833009
1105	10-11	0.3287772425702684
1105	12-13	0.3058604596622416
1105	14-15	0.38589059819088334
1105	16-17	0.3770199419433382
1105	18-19	0.2893645652640302
1105	20-21	0.3113338045746943
1105	22-23	0.3533101693114702
1105	24-25	0.310772029082635
1105	26-27	0.31215027574556586
1105	28-29	0.35996553393133723
1105	30-31	0.3619085054292057
1105	32-33	0.3673785041693094
1105	34-35	0.43564436205809187
1105	36-37	0.38426935542476315
1105	38-39	0.3822565915667582
1105	40-41	0.4613966565487786
1105	42-43	0.4797708306205024
1105	44-45	0.418731697569271
1105	46-47	0.3559311181038183
1105	48-49	0.43254483389989673
1105	50-51	0.48246778777214416
1105	52-53	0.4085950394154523
1105	54-55	0.3763102674716521
1105	56-57	0.3602276254069423
1105	58-59	0.43887819714525733
1105	60-61	0.3818950132750132
1105	62-63	0.35696395249498636
1105	64-65	0.3683938980061896
1105	66-67	0.41394682481158185
1105	68-69	0.37523122974198486
1105	70-71	0.4346074241044988
1105	72-73	0.401685513796707
1105	74-75	0.46576513018755605
1105	76-77	0.5546949169039408
1105	78-79	0.4976836560101887
1105	80-81	0.5150364932038798
1105	82-83	0.5225215158677159
1105	84-85	0.5220832991783233
1105	86-87	0.53492236314991
1105	88-89	0.48822837901816385
1105	90-91	0.5245529232769748
1105	92-93	0.5710686764691495
1105	94-95	0.5287008475065313
1105	96-97	0.5217687381413683
1105	98-99	0.5676045540348085
1105	100	0.5788771490163818
1106	1	0.09398988339268755
1106	2	0.10819371526572397
1106	3	0.14539716818902804
1106	4	0.17540525904383486
1106	5	0.13287074274040833
1106	6	0.2636446242487409
1106	7	0.2641940162317411
1106	8	0.22786589903881094
1106	9	0.33890890436236276
1106	10-11	0.30033213106283085
1106	12-13	0.3031114131753583
1106	14-15	0.37104819866580385
1106	16-17	0.39580800756915124
1106	18-19	0.3737604203418172
1106	20-21	0.29644545124821065
1106	22-23	0.323570348274842
1106	24-25	0.33756881349468415
1106	26-27	0.32591895812577576
1106	28-29	0.37225531076056484
1106	30-31	0.36110589369669555
1106	32-33	0.3460729461394365
1106	34-35	0.3919932013333707
1106	36-37	0.30175757050307084
1106	38-39	0.3933924953973502
1106	40-41	0.4348093150806207
1106	42-43	0.4010114356921761
1106	44-45	0.3283229039050539
1106	46-47	0.2771188849317454
1106	48-49	0.27708378422415336
1106	50-51	0.3162541911948722
1106	52-53	0.3069967896465755
1106	54-55	0.3499155951850952
1106	56-57	0.2441464606283219
1106	58-59	0.3259637806410183
1106	60-61	0.2821584309609122
1106	62-63	0.29196123233697335
1106	64-65	0.2911800990608242
1106	66-67	0.2976992433661607
1106	68-69	0.38031438184550126
1106	70-71	0.3662535275904588
1106	72-73	0.3928264265499948
1106	74-75	0.3931143122568983
1106	76-77	0.41900672942290385
1106	78-79	0.3809544611132907
1106	80-81	0.3980561976441166
1106	82-83	0.37077342981110917
1106	84-85	0.36373252714196624
1106	86-87	0.4030208093663816
1106	88-89	0.38197919754979637
1106	90-91	0.40637397311694556
1106	92-93	0.4322071002317429
1106	94-95	0.4698219369930321
1106	96-97	0.3894653280932161
1106	98-99	0.4213734649047254
1106	100	0.3784396269372259
1107	1	0.07358954385906458
1107	2	0.08526609710011712
1107	3	0.016427978932579634
1107	4	0.08215692568656863
1107	5	0.11250806876742558
1107	6	0.2121546597060373
1107	7	0.19794785384087277
1107	8	0.2881385764679507
1107	9	0.3181037854482014
1107	10-11	0.2954811241343194
1107	12-13	0.2890158265677343
1107	14-15	0.2830526829321798
1107	16-17	0.3032201046484033
1107	18-19	0.3319241308257759
1107	20-21	0.31858271087349266
1107	22-23	0.2641705168471802
1107	24-25	0.24885226298254537
1107	26-27	0.2799556709775288
1107	28-29	0.3676851333063169
1107	30-31	0.3063963110916532
1107	32-33	0.3125462753705861
1107	34-35	0.29082373875023393
1107	36-37	0.27608187699920705
1107	38-39	0.3068450565802294
1107	40-41	0.32717883240421486
1107	42-43	0.29307397111445255
1107	44-45	0.25804699311674284
1107	46-47	0.29352823228509095
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2214	100	-0.30742474472182835
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2215	4	-0.31853564058341277
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2215	22-23	-0.6678412985419442
2215	24-25	-0.7251019485197716
2215	26-27	-0.6457515762701433
2215	28-29	-0.5898879093441138
2215	30-31	-0.599220637550637
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2215	100	-0.48351885953466933
2216	1	-1.0057744490244467
2216	2	-0.9973053847329005
2216	3	-1.0005524427038388
2216	4	-1.3179154298729898
2216	5	-1.2702791430634761
2216	6	-2.8157397091970893
2216	7	-2.7751493579149695
2216	8	-3.5102123090265245
2216	9	-2.969988116086135
2216	10-11	-2.1706463871932797
2216	12-13	-1.9453571873688134
2216	14-15	-2.1308491571940067
2216	16-17	-2.5545554857712105
2216	18-19	-2.1523348693801765
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2216	26-27	-2.389629658765685
2216	28-29	-2.792877451990627
2216	30-31	-2.447579023857422
2216	32-33	-2.614256873602592
2216	34-35	-3.6703914758029725
2216	36-37	-2.658296365415673
2216	38-39	-2.487613548112151
2216	40-41	-3.00972574699221
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2216	50-51	-2.9037092395547006
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2216	54-55	-2.6471019607665554
2216	56-57	-2.690141201192631
2216	58-59	-3.7406589286192613
2216	60-61	-4.380443290720557
2216	62-63	-3.4152237053459658
2216	64-65	-3.399440772008866
2216	66-67	-3.0659660509187106
2216	68-69	-3.4702305037115586
2216	70-71	-3.261811700750542
2216	72-73	-3.7183021657584376
2216	74-75	-4.192311212512777
2216	76-77	-4.832439580499006
2216	78-79	-3.8380578286427536
2216	80-81	-3.654529175711307
2216	82-83	-3.8996007633673813
2216	84-85	-3.965225147800492
2216	86-87	-3.5854411450456247
2216	88-89	-3.547367636269886
2216	90-91	-3.6813786707179474
2216	92-93	-3.744077524569672
2216	94-95	-3.5957088175126906
2216	96-97	-3.9152217060397767
2216	98-99	-3.670220549146947
2216	100	-3.2554010664545068
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	79365.0
3	13364.0
4	9079.0
5	7602.0
6	6950.0
7	7432.0
8	8279.0
9	9600.0
10	10757.0
11	12040.0
12	13705.0
13	15663.0
14	17486.0
15	18738.0
16	18484.0
17	19303.0
18	18344.0
19	17135.0
20	17479.0
21	18315.0
22	20273.0
23	24330.0
24	29849.0
25	37266.0
26	45330.0
27	53366.0
28	66104.0
29	83492.0
30	101840.0
31	122035.0
32	156361.0
33	207268.0
34	287111.0
35	415628.0
36	680591.0
37	1194631.0
38	1605788.0
39	592357.0
40	1392.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.982708857669685	20.65448721966216	14.225477551532023	23.13732637113613
2	15.279489929250134	20.401625783706233	40.84073057001571	23.478153717027922
3	18.115519700744592	25.4813320864358	30.62217800490566	25.78097020791395
4	12.425389594085738	17.000251739493503	39.36443531334308	31.209923353077674
5	12.870807406076642	38.030453978779114	35.91701034252766	13.181728272616578
6	30.568423198975342	38.27450840653043	17.86177379474723	13.295294599747
7	26.51047045276831	32.868758887594815	22.985769950278957	17.635000709357914
8	24.36810377849344	36.89883292746057	20.85180821513753	17.881255078908463
9	24.492145852349186	17.151984986709458	22.016523395826574	36.339345765114786
10-11	23.421964206058654	27.293845334593243	29.431516141718774	19.85267431762933
12-13	23.816620222417693	24.88208846689107	29.41992969684652	21.881361613844717
14-15	21.26459530869894	27.18593614327935	26.503230497886243	25.046238050135468
16-17	20.82425255386999	30.528508994229735	27.1867134178172	21.46052503408308
18-19	20.732739055916973	29.470321170615378	29.17738358525086	20.61955618821679
20-21	21.845663798391573	28.721011823128745	28.673295677857542	20.76002870062214
22-23	21.545650525682316	28.53308799512227	28.70848864931351	21.212772829881903
24-25	21.352468589595127	28.67820608498444	28.670659075777582	21.29866624964286
26-27	21.132102301314383	28.631218931530018	28.769126894131226	21.467551873024377
28-29	21.155781298457924	28.667007040627123	28.885962625949364	21.29124903496559
30-31	21.438500433564	29.035854790001693	28.446969881303353	21.078674895130963
32-33	21.120999066888498	28.776423003289437	28.820212351532543	21.28236557828952
34-35	21.288629216270824	28.48476210460192	28.88262637100148	21.343982308125778
36-37	21.512231972102853	28.832717171559715	28.376078654260787	21.27897220207665
38-39	21.40412624031803	28.925738578079606	28.504911124511302	21.165224057091063
40-41	21.27676298720553	28.64754871447015	28.711908306016454	21.36377999230786
42-43	21.211808036270295	28.649245361422004	28.66290412750425	21.476042474803453
44-45	21.29572642410328	28.730130896603214	28.584950494984174	21.389192184309334
46-47	21.301582957704298	28.817941182559466	28.478648179210573	21.40182768052566
48-49	21.25833510525372	28.706710362855752	28.51780907831382	21.517145453576703
50-51	21.31192796679893	28.633086903064097	28.472985446364362	21.581999683772608
52-53	21.265253150018538	28.593602760114432	28.529499217882346	21.61164487198468
54-55	21.328462966691347	28.92534744746605	28.3421587902615	21.404030795581104
56-57	21.268225428940603	28.700432829451607	28.36603294214991	21.665308799457886
58-59	21.175391861345723	28.353290751130334	28.665621824047115	21.805695563476828
60-61	21.345569835323538	28.676616866182027	28.37161157931375	21.606201719180692
62-63	21.23463164401871	28.738245241289324	28.31488216476849	21.71224094992348
64-65	21.269421714447084	28.489343490668883	28.43405965771365	21.807175137170383
66-67	21.125530492777646	28.51126505372229	28.3355475688387	22.02765688466137
68-69	21.44887867921783	28.61526442597158	28.269945726206842	21.665911168603742
70-71	21.38817086347253	28.39058749783493	28.367790305419692	21.85345133327285
72-73	21.19472718281297	28.506073069498928	28.484216815360675	21.81498293232743
74-75	21.257490956417605	28.512646169529436	28.364138422482522	21.86572445157044
76-77	21.333352688100916	28.4828581229279	28.201295741206085	21.982493447765098
78-79	21.3273764558674	28.55673809492586	28.30245075394205	21.813434695264693
80-81	21.308177239757853	28.39408573975018	28.356863457319083	21.940873563172886
82-83	21.146959199073027	28.438830940370984	28.4947500756929	21.919459784863086
84-85	21.238355089689037	28.308343855414115	28.333969648075474	22.119331406821377
86-87	21.353888646586427	28.438983988022855	28.270679085455935	21.936448279934783
88-89	21.254903235155773	28.31471233240295	28.34048681859785	22.08989761384343
90-91	21.249602348865558	28.1818008612606	28.341620120417467	22.226976669456377
92-93	21.31351111809788	28.310098444382625	28.209030450795087	22.167359986724414
94-95	21.215088689998307	28.126023249380626	28.37326091937508	22.285627141245985
96-97	21.27090076535273	28.091093663528433	28.356251150205836	22.281754420913003
98-99	21.303197225917906	28.157335625279924	28.2671040142266	22.272363134575567
100	21.351613058554793	28.0361476300318	28.23642361347016	22.37581569794325
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1950.0
1	1499.0
2	1310.5
3	1598.0
4	1512.5
5	1246.0
6	1045.0
7	1004.0
8	1000.5
9	975.5
10	874.0
11	784.0
12	814.5
13	899.0
14	1008.0
15	1161.0
16	1418.0
17	1860.0
18	2551.5
19	3526.5
20	5269.5
21	7928.5
22	11447.0
23	16837.5
24	24351.0
25	33853.5
26	46652.0
27	62135.5
28	80012.5
29	100429.5
30	120774.0
31	138256.0
32	152119.5
33	165424.0
34	179241.5
35	190652.0
36	199714.5
37	209463.5
38	218746.5
39	222886.5
40	224652.5
41	224959.0
42	223115.5
43	219837.5
44	214045.0
45	208833.0
46	203989.5
47	196278.5
48	188149.5
49	181713.0
50	176202.0
51	168872.5
52	156952.0
53	146384.0
54	137965.5
55	129967.0
56	120760.0
57	109595.0
58	99602.5
59	90395.5
60	79031.5
61	66636.5
62	56763.0
63	47740.5
64	39147.0
65	31974.5
66	25684.0
67	19890.5
68	15277.0
69	11857.0
70	9185.0
71	7022.5
72	5128.0
73	3592.0
74	2651.5
75	1989.5
76	1388.0
77	977.5
78	674.0
79	491.0
80	372.5
81	246.0
82	181.0
83	140.5
84	106.0
85	81.0
86	66.0
87	56.0
88	41.5
89	32.0
90	28.5
91	23.5
92	15.0
93	12.5
94	11.0
95	9.5
96	9.5
97	11.5
98	13.5
99	11.5
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015039250464864552
2	0.034151631263963256
3	0.03550384457330414
4	0.0380928383485056
5	0.04058288968643822
6	0.03984082140692188
7	0.038125819160928553
8	0.037894953473967916
9	0.037977405505025284
10-11	0.03832370403546625
12-13	0.03931312840815471
14-15	0.03785372745843923
16-17	0.03473704068447059
18-19	0.037276563241037626
20-21	0.03697973592923109
22-23	0.037861972661544965
24-25	0.03509158441801728
26-27	0.03492668035590254
28-29	0.035817162291322154
30-31	0.03572646505715905
32-33	0.03554507058883283
34-35	0.03528946929255498
36-37	0.03308800006332316
38-39	0.03343429859376412
40-41	0.034605117434778794
42-43	0.03448143938819274
44-45	0.03329413014096659
46-47	0.03244487422107566
48-49	0.03250259064281582
50-51	0.033615693062090335
52-53	0.03404444362358867
54-55	0.0334507889999756
56-57	0.031809993581933906
58-59	0.03256855226766172
60-61	0.03202436886268307
62-63	0.03231295097138387
64-65	0.02936941346263571
66-67	0.03040830905395859
68-69	0.03143071423906999
70-71	0.03268398511114204
72-73	0.029583788743384874
74-75	0.030045520117306154
76-77	0.031125641724157722
78-79	0.031257564973849516
80-81	0.03138124302043557
82-83	0.029789918821028303
84-85	0.031010208880677397
86-87	0.031661579926030636
88-89	0.03191718122230848
90-91	0.03251083584592156
92-93	0.030944247255831503
94-95	0.03213980170616339
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6064132.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.6979212959316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.63912393665849	48.051137607697626
2	14.022847332330016	17.584067567745834
3	4.350939014089251	8.183844956062984
4	1.7939660770601082	4.499117756283434
5	0.9435932282613441	2.958066698045189
6	0.5594867368426376	2.104719323560637
7	0.3410695261730175	1.4969045215905574
8	0.2528803022000258	1.2684055427702894
9	0.17229420253654532	0.9722239515343456
>10	0.8668692333455201	9.500100366002178
>50	0.04263498145887874	1.8283346971068477
>100	0.014111239583973484	1.4415557771712602
>500	1.3156629460818597E-4	0.05373289007625819
>1k	5.262316560223472E-5	0.05778834435260811
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.2980812422948576E-5	0.0	0.0	0.0
3	0.0	3.2980812422948576E-5	0.0	4.9471218634422864E-5	0.0
4	0.0	3.2980812422948576E-5	0.0	8.245203105737144E-5	0.0
5	0.0	3.2980812422948576E-5	0.0	1.319232496917943E-4	0.0
6	0.0	3.2980812422948576E-5	0.0	1.319232496917943E-4	0.0
7	0.0	3.2980812422948576E-5	0.0	1.319232496917943E-4	0.0
8	0.0	3.2980812422948576E-5	0.0	1.319232496917943E-4	0.0
9	0.0	3.2980812422948576E-5	0.0	1.9788487453769146E-4	0.0
10-11	0.0	4.9471218634422864E-5	0.0	2.3086568696064003E-4	0.0
12-13	0.0	5.771642174016001E-5	0.0	2.391108900663772E-4	0.0
14-15	0.0	1.1543284348032002E-4	0.0	2.968273118065372E-4	0.0
16-17	0.0	1.4016845279753143E-4	0.0	3.2980812422948576E-4	0.0
18-19	0.0	1.484136559032686E-4	0.0	3.4629853044096E-4	0.0
20-21	0.0	1.6490406211474288E-4	0.0	4.3699576460406867E-4	0.0
22-23	0.0	1.6490406211474288E-4	0.0	6.596162484589715E-4	0.0
24-25	0.0	1.8139446832621717E-4	0.0	0.0011625736379089373	0.0
26-27	0.0	2.3086568696064003E-4	0.0	0.0021025267919629717	0.0
28-29	0.0	2.638464993835886E-4	0.0	0.005177987550402927	0.0
30-31	0.0	2.638464993835886E-4	0.0	0.015286606558036667	0.0
32-33	0.0	2.803369055950629E-4	0.0	0.038777190206281786	0.0
34-35	0.0	2.968273118065372E-4	0.0	0.07792541455232174	0.0
36-37	0.0	3.133177180180115E-4	0.0	0.1387585230664504	0.0
38-39	0.0	3.627889366524344E-4	0.0	0.21768160719456636	0.0
40-41	0.0	3.7927934286390865E-4	0.0	0.2984433716152617	0.0
42-43	0.0	3.7927934286390865E-4	0.0	0.37955143456639795	0.0
44-45	8.245203105737144E-6	3.875245459696458E-4	0.0	0.47701798047931676	0.0
46-47	1.6490406211474288E-5	3.957697490753829E-4	0.0	0.5937205852379203	0.0
48-49	1.6490406211474288E-5	3.957697490753829E-4	0.0	0.7157578364059358	0.0
50-51	1.6490406211474288E-5	4.1226015528685717E-4	0.0	0.8357667676099398	0.0
52-53	1.6490406211474288E-5	4.452409677098058E-4	0.0	0.9515871356362295	0.0
54-55	3.2980812422948576E-5	4.452409677098058E-4	0.0	1.0811769928491002	0.0
56-57	3.2980812422948576E-5	4.699765770270172E-4	0.0	1.2310830305145073	0.0
58-59	3.2980812422948576E-5	4.947121863442287E-4	0.0	1.3921283375757652	0.0
60-61	3.2980812422948576E-5	4.947121863442287E-4	0.0	1.5569994188780851	0.0
62-63	3.2980812422948576E-5	4.947121863442287E-4	0.0	1.7181024423610833	0.0
64-65	3.2980812422948576E-5	4.947121863442287E-4	0.0	1.8904024516616724	0.0
66-67	3.2980812422948576E-5	4.947121863442287E-4	0.0	2.083785445303631	0.0
68-69	3.2980812422948576E-5	4.947121863442287E-4	0.0	2.294475120264533	0.0
70-71	3.2980812422948576E-5	5.029573894499658E-4	0.0	2.5172769985877617	0.0
72-73	3.2980812422948576E-5	5.11202592555703E-4	0.0	2.742709096701721	0.0
74-75	3.2980812422948576E-5	5.276929987671772E-4	0.0	2.9688420370796678	0.0
76-77	3.2980812422948576E-5	5.524286080843886E-4	0.0	3.2100719443442194	0.0
78-79	3.2980812422948576E-5	5.771642174016001E-4	0.0	3.4803991733689177	0.0
80-81	3.2980812422948576E-5	5.771642174016001E-4	0.0	3.768503060289585	0.0
82-83	3.2980812422948576E-5	5.771642174016001E-4	0.0	4.064662510644557	0.0
84-85	3.2980812422948576E-5	5.771642174016001E-4	0.0	4.356006762385779	0.0
86-87	3.2980812422948576E-5	5.854094205073372E-4	0.0	4.670858088181458	0.0
88	3.2980812422948576E-5	5.936546236130744E-4	0.0	4.921462791377232	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5715	0.0	43.598003	1
GTATCAA	9625	0.0	35.41149	1
TCAACGC	9930	0.0	32.9487	4
ATCAACG	10165	0.0	32.23322	3
CAACGCA	10635	0.0	30.985523	5
AACGCAG	11245	0.0	29.258524	6
TATCAAC	11705	0.0	28.634949	2
ACGCAGA	13445	0.0	24.156336	7
GTACATG	15710	0.0	23.939812	1
CGCAGAG	13795	0.0	23.270689	8
TACATGG	15930	0.0	22.810879	2
ACATGGG	16480	0.0	21.678772	3
GCAGAGT	15700	0.0	19.638777	9
GTGGTAT	3635	0.0	19.011652	1
CATGGGG	11605	0.0	17.863714	4
GAGTACA	12110	0.0	17.585754	1
CAGAGTA	14780	0.0	17.363148	10-11
AGAGTAC	13485	0.0	17.026442	10-11
TGGTATC	3525	0.0	16.403038	2
GTATAGA	2945	0.0	15.1651	1
>>END_MODULE