FastQCFastQC Report
Wed 25 May 2016
SRR2049355_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049355_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6064132
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144000.23746184944522974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139800.23053587883641058No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78270.12907040941720926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101000.058.18181
GTATCAA180100.039.5716361
TCAACGC217900.032.0925754
ATCAACG219250.031.9592743
CAACGCA225400.031.045575
TATCAAC233100.030.1208462
AACGCAG233150.030.119316
GTGGTAT43250.028.3695811
TGGTATC43300.026.1570032
ACGCAGA270850.025.5798597
CGCAGAG272200.025.2974788
GTACATG159150.024.930691
TACATGG160250.023.5198842
GCAGAGT299200.022.6687689
ACATGGG165150.022.1675513
GAGTACT215550.020.97222912-13
CAGAGTA293550.020.75771710-11
AGAGTAC274600.020.36599310-11
CCGGTCG5350.020.2207989
CATGGGG116650.019.7410474
Produced by FastQC (version 0.11.5)