##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049355_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6064132 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.884701883138426 33.0 31.0 34.0 30.0 34.0 2 31.999504463293345 33.0 31.0 34.0 30.0 34.0 3 31.974547717628838 33.0 31.0 34.0 30.0 34.0 4 35.69695382620299 37.0 35.0 37.0 33.0 37.0 5 35.791592927066894 37.0 35.0 37.0 35.0 37.0 6 35.84334410926411 37.0 35.0 37.0 35.0 37.0 7 35.784981263600464 37.0 35.0 37.0 33.0 37.0 8 35.77790786876011 37.0 35.0 37.0 33.0 37.0 9 37.494825805243025 39.0 37.0 39.0 35.0 39.0 10-11 37.46908444605097 39.0 37.0 39.0 34.5 39.0 12-13 37.42530612790091 39.0 37.0 39.0 34.0 39.0 14-15 38.714688598467184 40.0 38.0 41.0 34.0 41.0 16-17 38.742165737817054 40.0 38.0 41.0 34.5 41.0 18-19 38.813695430772285 40.0 38.0 41.0 35.0 41.0 20-21 38.84546205458588 40.0 38.0 41.0 35.0 41.0 22-23 38.813267339827036 40.0 38.0 41.0 35.0 41.0 24-25 38.7958470396093 40.0 38.0 41.0 35.0 41.0 26-27 38.68275781265975 40.0 38.0 41.0 34.0 41.0 28-29 38.60327850713012 40.0 38.0 41.0 34.0 41.0 30-31 38.50228309673997 40.0 38.0 41.0 34.0 41.0 32-33 38.36632151147106 40.0 38.0 41.0 34.0 41.0 34-35 38.27340442127579 40.0 38.0 41.0 34.0 41.0 36-37 38.16459734055921 40.0 38.0 41.0 34.0 41.0 38-39 38.07293690176929 40.0 38.0 41.0 33.5 41.0 40-41 37.96776710995077 40.0 38.0 41.0 33.0 41.0 42-43 37.835425993365575 40.0 37.0 41.0 33.0 41.0 44-45 37.66154010169963 40.0 37.0 41.0 33.0 41.0 46-47 37.44187032868018 40.0 37.0 41.0 32.5 41.0 48-49 37.24247979760335 40.0 37.0 41.0 32.0 41.0 50-51 37.09971344621126 40.0 37.0 41.0 31.5 41.0 52-53 36.96717757792871 40.0 36.5 41.0 31.0 41.0 54-55 36.83000518458371 40.0 36.0 41.0 31.0 41.0 56-57 36.69532053391978 39.0 36.0 41.0 31.0 41.0 58-59 36.51144681547169 39.0 35.0 41.0 31.0 41.0 60-61 36.3379853044096 39.0 35.0 41.0 31.0 41.0 62-63 36.14442231798384 39.0 35.0 41.0 30.0 41.0 64-65 35.92443287843998 38.5 35.0 40.5 30.0 41.0 66-67 35.66316927138129 38.0 35.0 40.0 30.0 41.0 68-69 35.37950757008588 37.0 35.0 40.0 29.0 41.0 70-71 35.05045297496822 37.0 34.5 39.5 29.0 41.0 72-73 34.75594858423266 36.0 34.0 39.0 29.0 41.0 74-75 34.45114725405054 36.0 34.0 39.0 29.0 41.0 76-77 34.123496569665704 35.5 34.0 38.5 29.0 40.0 78-79 33.78430169396049 35.0 34.0 37.0 29.0 39.5 80-81 33.46146472405284 35.0 34.0 37.0 28.0 39.0 82-83 33.154705652845294 35.0 34.0 36.5 28.0 39.0 84-85 32.87720798293968 35.0 33.5 36.0 28.0 37.5 86-87 32.63734142000867 35.0 33.0 36.0 27.0 37.0 88-89 32.404430757773746 35.0 33.0 35.5 27.0 37.0 90-91 32.204771268171605 35.0 33.0 35.0 27.0 36.0 92-93 32.01923160643601 35.0 33.0 35.0 26.0 36.0 94-95 31.85863558049198 35.0 33.0 35.0 26.0 36.0 96-97 31.65671871918355 35.0 33.0 35.0 25.5 36.0 98-99 31.509316254989173 35.0 33.0 35.0 25.0 35.5 100 31.395713022078017 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22746748623705315 1101 2 0.23164519803163586 1101 3 0.38487979999638 1101 4 0.2172101232915864 1101 5 0.20807018818593548 1101 6 0.21036371303104318 1101 7 0.07748628263510682 1101 8 0.16123135872133787 1101 9 0.291019495542713 1101 10-11 0.23588037521306404 1101 12-13 0.19991500368745818 1101 14-15 0.3218238586171296 1101 16-17 0.2252719512933936 1101 18-19 0.3139599943502418 1101 20-21 0.28184953750940167 1101 22-23 0.2716157382026978 1101 24-25 0.2882975162035635 1101 26-27 0.34785780340803285 1101 28-29 0.3479641872983663 1101 30-31 0.3557790814391453 1101 32-33 0.3815337870067097 1101 34-35 0.3611789014003932 1101 36-37 0.37359169035969586 1101 38-39 0.36584283948503327 1101 40-41 0.36212718951764344 1101 42-43 0.37484300227691136 1101 44-45 0.3764668596628127 1101 46-47 0.40552040495989417 1101 48-49 0.4076067524748197 1101 50-51 0.2975145304751621 1101 52-53 0.3327581492348699 1101 54-55 0.3378816717537916 1101 56-57 0.3606962910043592 1101 58-59 0.3967570039460213 1101 60-61 0.3985977356985799 1101 62-63 0.3827234004263289 1101 64-65 0.31924610074632653 1101 66-67 0.33094247421807665 1101 68-69 0.3778419485256066 1101 70-71 0.4192334148686001 1101 72-73 0.3434322909206955 1101 74-75 0.4134098323470994 1101 76-77 0.3285367506150152 1101 78-79 0.37698002081287285 1101 80-81 0.3740281287827898 1101 82-83 0.36539755562719733 1101 84-85 0.41910191118955 1101 86-87 0.2985156497745649 1101 88-89 0.3809083784701528 1101 90-91 0.4131008231409865 1101 92-93 0.4101498199671596 1101 94-95 0.4690882800993741 1101 96-97 0.5277827849813512 1101 98-99 0.5733892305084609 1101 100 0.5863466193700972 1102 1 0.16946883510623678 1102 2 0.20949548575270427 1102 3 0.36218552069107446 1102 4 0.21300032296062454 1102 5 0.1904392118004523 1102 6 0.1970809734762753 1102 7 0.17449558181401414 1102 8 0.15588652947427306 1102 9 0.1606782963878146 1102 10-11 0.19211550877087546 1102 12-13 0.20784174175253156 1102 14-15 0.30703206474354516 1102 16-17 0.23017062855804227 1102 18-19 0.2365127458139824 1102 20-21 0.22838561099259636 1102 22-23 0.2846285918430098 1102 24-25 0.289762768237793 1102 26-27 0.3522629938489388 1102 28-29 0.30389733691131937 1102 30-31 0.2678553904525103 1102 32-33 0.28859338312538085 1102 34-35 0.33900495067507563 1102 36-37 0.31931755280752583 1102 38-39 0.34084271570367974 1102 40-41 0.3023825190285052 1102 42-43 0.3198707946314201 1102 44-45 0.346681171366626 1102 46-47 0.3254805719071072 1102 48-49 0.28217841046584624 1102 50-51 0.3490849498439772 1102 52-53 0.37955422598589195 1102 54-55 0.30042211403074504 1102 56-57 0.371425375101353 1102 58-59 0.33446288443713 1102 60-61 0.2919906572578981 1102 62-63 0.33738862463706454 1102 64-65 0.31401609377952155 1102 66-67 0.2794412234447279 1102 68-69 0.3189709545034489 1102 70-71 0.3733604774720689 1102 72-73 0.3514256155518325 1102 74-75 0.32340257131154715 1102 76-77 0.31768328221676256 1102 78-79 0.3601701652463518 1102 80-81 0.34128007247282 1102 82-83 0.37502516300340716 1102 84-85 0.385275786667151 1102 86-87 0.35776428687800177 1102 88-89 0.33769320764571376 1102 90-91 0.38014570084082067 1102 92-93 0.4537574167005971 1102 94-95 0.4020090642458207 1102 96-97 0.4467499062559064 1102 98-99 0.4153408980107187 1102 100 0.44010518381622177 1103 1 0.13172275210947504 1103 2 0.09876124946273279 1103 3 0.2753920750526042 1103 4 0.14004517976715647 1103 5 0.1431079221517635 1103 6 0.060084496540490306 1103 7 0.07374640054053572 1103 8 0.13617958215004933 1103 9 0.1676909077121138 1103 10-11 0.12988675015000695 1103 12-13 0.17213193930818704 1103 14-15 0.23258758231182952 1103 16-17 0.17238669562458142 1103 18-19 0.19031181570748856 1103 20-21 0.21000390475411734 1103 22-23 0.20150946902646183 1103 24-25 0.1787662119626603 1103 26-27 0.18464481231353602 1103 28-29 0.24555107439388024 1103 30-31 0.23629317206692235 1103 32-33 0.2853614833379652 1103 34-35 0.27624127496689255 1103 36-37 0.2415333978883396 1103 38-39 0.237192687986294 1103 40-41 0.24893968826651758 1103 42-43 0.22325015734465126 1103 44-45 0.2415490943126457 1103 46-47 0.29985190182016197 1103 48-49 0.23523683594437728 1103 50-51 0.23288213628452326 1103 52-53 0.1819424498349278 1103 54-55 0.13196094854949791 1103 56-57 0.16751656957845995 1103 58-59 0.22063637327100594 1103 60-61 0.20607836477773134 1103 62-63 0.17741399404289382 1103 64-65 0.20746726819021433 1103 66-67 0.17593103988377123 1103 68-69 0.15013625692851207 1103 70-71 0.2028415728181585 1103 72-73 0.20433704400052477 1103 74-75 0.21891593146084887 1103 76-77 0.23422196566190223 1103 78-79 0.2309351314863619 1103 80-81 0.303086606019761 1103 82-83 0.25679264624639586 1103 84-85 0.29157311750118 1103 86-87 0.21529041490635592 1103 88-89 0.24529045674925243 1103 90-91 0.2710788651178717 1103 92-93 0.2851357637606142 1103 94-95 0.25204278346662434 1103 96-97 0.3070512875072815 1103 98-99 0.2823826429062315 1103 100 0.3545892249266096 1104 1 0.09762564500939774 1104 2 0.12501590347623193 1104 3 0.24796084361555515 1104 4 0.1336210964509661 1104 5 0.12540598296059358 1104 6 0.12054935322711202 1104 7 0.13968839493474405 1104 8 0.13037040676609024 1104 9 0.16630379287250463 1104 10-11 0.17011933805105173 1104 12-13 0.12521903675403223 1104 14-15 0.2007505350150396 1104 16-17 0.20774982440599388 1104 18-19 0.19667687579410398 1104 20-21 0.21027467357354368 1104 22-23 0.15635693478671442 1104 24-25 0.18606309578697022 1104 26-27 0.1975797770404455 1104 28-29 0.22611679126741535 1104 30-31 0.2535954092830508 1104 32-33 0.22312111020136882 1104 34-35 0.2050995500742374 1104 36-37 0.2488590874650285 1104 38-39 0.2140406045792247 1104 40-41 0.22756347588217096 1104 42-43 0.25871007791904077 1104 44-45 0.20014755886820268 1104 46-47 0.26461153400215665 1104 48-49 0.21694053434165284 1104 50-51 0.21378172935975925 1104 52-53 0.21732137680194086 1104 54-55 0.1929786169749974 1104 56-57 0.21969239537345686 1104 58-59 0.28818874514269766 1104 60-61 0.20879759628464 1104 62-63 0.240712615798941 1104 64-65 0.16064131475231136 1104 66-67 0.23550682511301346 1104 68-69 0.20865491635199618 1104 70-71 0.29932660784567844 1104 72-73 0.2530815971229714 1104 74-75 0.24723124407221064 1104 76-77 0.16740933759301413 1104 78-79 0.22647259055372615 1104 80-81 0.20719194774351024 1104 82-83 0.23633825733512026 1104 84-85 0.18696004730634996 1104 86-87 0.18371889006561304 1104 88-89 0.19348203397878194 1104 90-91 0.17369263093189602 1104 92-93 0.2759213439699977 1104 94-95 0.2819531415673744 1104 96-97 0.30116929995370256 1104 98-99 0.3597160352474731 1104 100 0.378540154159996 1105 1 0.22231547905884952 1105 2 0.21468156137161287 1105 3 0.32524611875439646 1105 4 0.22690414094842737 1105 5 0.20531714498347498 1105 6 0.17052320047278613 1105 7 0.15031247066522013 1105 8 0.16047834970456165 1105 9 0.24394622908528873 1105 10-11 0.22981848103967195 1105 12-13 0.20610908031130748 1105 14-15 0.2840547148147792 1105 16-17 0.2564690290640499 1105 18-19 0.2378724061364892 1105 20-21 0.28265125782812106 1105 22-23 0.24865311431010895 1105 24-25 0.2726871377198492 1105 26-27 0.28146060969642406 1105 28-29 0.2688021246449779 1105 30-31 0.23747526031765887 1105 32-33 0.30158887875666807 1105 34-35 0.30604797649479565 1105 36-37 0.2847775766080787 1105 38-39 0.29315133513602376 1105 40-41 0.3219520994122931 1105 42-43 0.30525563735904626 1105 44-45 0.30745597231395294 1105 46-47 0.31331197602378325 1105 48-49 0.2986104263800726 1105 50-51 0.22400310423979164 1105 52-53 0.2262519071464908 1105 54-55 0.25627609814075925 1105 56-57 0.19404989156547714 1105 58-59 0.29810300921338495 1105 60-61 0.27374197718256 1105 62-63 0.30611991194211896 1105 64-65 0.24513674179458178 1105 66-67 0.2299581046658048 1105 68-69 0.21511171733863677 1105 70-71 0.21382518663810401 1105 72-73 0.21961957198463722 1105 74-75 0.20956507126049928 1105 76-77 0.2501851130909927 1105 78-79 0.26662256987444266 1105 80-81 0.25066039131882434 1105 82-83 0.28586065815238015 1105 84-85 0.2748850171385797 1105 86-87 0.2565649564882051 1105 88-89 0.25047502079107176 1105 90-91 0.29554075186317164 1105 92-93 0.3627777257421769 1105 94-95 0.36408093512668316 1105 96-97 0.41880182924593967 1105 98-99 0.45299113358201737 1105 100 0.4454086869137477 1106 1 0.1295295594511643 1106 2 0.1342635870625486 1106 3 0.2775594966658339 1106 4 0.1660277008608375 1106 5 0.09817918022336869 1106 6 0.08872364889572282 1106 7 0.15090653199173687 1106 8 0.12229998147924448 1106 9 0.12132886050004288 1106 10-11 0.13352199565756706 1106 12-13 0.12831955276791263 1106 14-15 0.20553277066968434 1106 16-17 0.20263822366581508 1106 18-19 0.19162711375327035 1106 20-21 0.22789224871418412 1106 22-23 0.17736179225975945 1106 24-25 0.1781254009029709 1106 26-27 0.1732468035512511 1106 28-29 0.1946470471366908 1106 30-31 0.19240432765303694 1106 32-33 0.17236866948508833 1106 34-35 0.1418964459321188 1106 36-37 0.17460180554905236 1106 38-39 0.21097257395602043 1106 40-41 0.25075422012994864 1106 42-43 0.25523743256098896 1106 44-45 0.2162865399996008 1106 46-47 0.23989695524163324 1106 48-49 0.26561869705277985 1106 50-51 0.23058459541952203 1106 52-53 0.21383077253921812 1106 54-55 0.224875942398846 1106 56-57 0.23394045094813265 1106 58-59 0.26140778906921724 1106 60-61 0.20794634420390423 1106 62-63 0.18512738480495017 1106 64-65 0.1386201916725298 1106 66-67 0.18117000155339724 1106 68-69 0.18492242611548448 1106 70-71 0.1965668893231296 1106 72-73 0.2943738875392725 1106 74-75 0.23236893266506797 1106 76-77 0.23481603290068165 1106 78-79 0.2689655532680604 1106 80-81 0.2838419932089593 1106 82-83 0.3348946911580697 1106 84-85 0.2688354904484527 1106 86-87 0.26105102453684026 1106 88-89 0.24304313734985783 1106 90-91 0.20619298940511044 1106 92-93 0.2712175819864484 1106 94-95 0.2283600986092189 1106 96-97 0.2973403388944824 1106 98-99 0.27402757953417733 1106 100 0.3062609689754794 1107 1 0.06592245923752671 1107 2 0.059679757971387914 1107 3 0.17162149711817065 1107 4 0.13896412651531165 1107 5 0.0903260916339832 1107 6 0.11299872362290131 1107 7 0.09461426095894154 1107 8 0.13952739704753014 1107 9 0.12483120939509007 1107 10-11 0.10368548250444576 1107 12-13 0.05078210915878856 1107 14-15 0.10553890167577862 1107 16-17 0.10153548943189605 1107 18-19 0.10286812095485232 1107 20-21 0.1010626942646411 1107 22-23 0.03783633096483641 1107 24-25 0.08952331100210387 1107 26-27 0.1431147341445751 1107 28-29 0.16760246887834285 1107 30-31 0.14202020134106164 1107 32-33 0.14818206624898522 1107 34-35 0.11684775745009546 1107 36-37 0.1775232077889939 1107 38-39 0.12635440154897992 1107 40-41 0.20794482586553187 1107 42-43 0.2644966107068498 1107 44-45 0.22984935547564334 1107 46-47 0.31422470168011074 1107 48-49 0.3360910718253223 1107 50-51 0.3159174627994972 1107 52-53 0.24306960008416212 1107 54-55 0.2535068368301623 1107 56-57 0.2766533133614857 1107 58-59 0.3527032523428417 1107 60-61 0.30398592423419046 1107 62-63 0.30130172075639905 1107 64-65 0.2946667197097952 1107 66-67 0.3068433259413226 1107 68-69 0.28660147608855624 1107 70-71 0.27307933742931567 1107 72-73 0.34246604873887065 1107 74-75 0.32285034623897957 1107 76-77 0.3227207966795831 1107 78-79 0.32088958744301266 1107 80-81 0.3442196069600314 1107 82-83 0.3327687741273593 1107 84-85 0.33475211709424 1107 86-87 0.2891494161264134 1107 88-89 0.27019801189454995 1107 90-91 0.26856238693146395 1107 92-93 0.2812074533450897 1107 94-95 0.3021411811682846 1107 96-97 0.3131929323374685 1107 98-99 0.29772558031177 1107 100 0.32159961240243007 1108 1 0.10142993387349719 1108 2 0.1068707017761028 1108 3 0.16089247067242596 1108 4 0.04036962462887317 1108 5 0.0675482415790043 1108 6 0.03314951042580816 1108 7 0.0701205733023258 1108 8 0.009329379245578195 1108 9 0.09979233307903002 1108 10-11 0.10729730845516627 1108 12-13 0.09835337632371477 1108 14-15 0.16245116033704932 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10-11 -0.3702464680193245 2215 12-13 -0.3591260229541078 2215 14-15 -0.3921331842797926 2215 16-17 -0.4245132716151403 2215 18-19 -0.44485179959129084 2215 20-21 -0.46371679921968223 2215 22-23 -0.3941690549478949 2215 24-25 -0.397551009349975 2215 26-27 -0.4558349127354191 2215 28-29 -0.42340462809089274 2215 30-31 -0.4707166699256149 2215 32-33 -0.40943946209761606 2215 34-35 -0.47640327776953484 2215 36-37 -0.4604589592194728 2215 38-39 -0.49716448239426114 2215 40-41 -0.470788805495701 2215 42-43 -0.48334155815869195 2215 44-45 -0.5046536408490141 2215 46-47 -0.5189019145543767 2215 48-49 -0.47963633603729505 2215 50-51 -0.5652215004362589 2215 52-53 -0.521501084809465 2215 54-55 -0.388523131708844 2215 56-57 -0.5008248130856217 2215 58-59 -0.5000714103765702 2215 60-61 -0.48838107990503943 2215 62-63 -0.40985154854873684 2215 64-65 -0.48896101480999477 2215 66-67 -0.44164899753408093 2215 68-69 -0.47987879388785615 2215 70-71 -0.5375184801810349 2215 72-73 -0.5132057635789948 2215 74-75 -0.47351989668397465 2215 76-77 -0.3602885078689866 2215 78-79 -0.3900315017933025 2215 80-81 -0.4924207002810377 2215 82-83 -0.4391933867367541 2215 84-85 -0.4525597991790278 2215 86-87 -0.36878713310966305 2215 88-89 -0.42201182749264277 2215 90-91 -0.3900213469745637 2215 92-93 -0.37773417958263167 2215 94-95 -0.46337352523668685 2215 96-97 -0.47936975018815176 2215 98-99 -0.43697679606451345 2215 100 -0.44226873504867825 2216 1 -0.3623700348988166 2216 2 -0.3829478151005823 2216 3 -0.4683941399906786 2216 4 -0.2517061191655898 2216 5 -0.4794531802101787 2216 6 -0.3557645063037782 2216 7 -0.5917734136925361 2216 8 -0.34570925149066056 2216 9 -0.5361626251133416 2216 10-11 -0.4237763981869236 2216 12-13 -0.43068782431460306 2216 14-15 -0.700423491529854 2216 16-17 -0.46438713957655864 2216 18-19 -0.4270126738519906 2216 20-21 -0.4508463481033118 2216 22-23 -0.4486721847714463 2216 24-25 -0.4251057246042933 2216 26-27 -0.5480273444331232 2216 28-29 -0.5845441427952096 2216 30-31 -0.6636049421593313 2216 32-33 -0.6109219586716819 2216 34-35 -0.6019844657698457 2216 36-37 -0.7107238875265196 2216 38-39 -0.6377071308033706 2216 40-41 -0.5445469116477852 2216 42-43 -0.6241098203934712 2216 44-45 -0.8142166814909899 2216 46-47 -0.8168498261913868 2216 48-49 -0.8456447531632634 2216 50-51 -0.7609247275349986 2216 52-53 -0.6547119827782737 2216 54-55 -0.6008699959005881 2216 56-57 -0.6804739303214831 2216 58-59 -0.6823743568863492 2216 60-61 -0.5149061411566507 2216 62-63 -0.475591386834175 2216 64-65 -0.5660525194350399 2216 66-67 -0.4767120866236567 2216 68-69 -0.417305528766569 2216 70-71 -0.5143346612995998 2216 72-73 -0.5349670007760068 2216 74-75 -0.5288934412542687 2216 76-77 -0.4624845683869694 2216 78-79 -0.6649646590324991 2216 80-81 -0.8042749460143241 2216 82-83 -0.7042613504420387 2216 84-85 -0.8541329348352917 2216 86-87 -0.6831189918446015 2216 88-89 -0.9785266455373005 2216 90-91 -0.789465203805257 2216 92-93 -0.9111952951585209 2216 94-95 -0.8182963442736337 2216 96-97 -0.8169431170901085 2216 98-99 -0.9705223488869201 2216 100 -0.9535408496140896 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 333.0 3 43.0 4 51.0 5 107.0 6 278.0 7 740.0 8 1824.0 9 3170.0 10 4695.0 11 6660.0 12 9302.0 13 12888.0 14 15905.0 15 20119.0 16 24802.0 17 33753.0 18 33596.0 19 19201.0 20 14566.0 21 14247.0 22 15384.0 23 17455.0 24 20538.0 25 25233.0 26 32610.0 27 42077.0 28 55645.0 29 73361.0 30 94384.0 31 123334.0 32 158946.0 33 208134.0 34 282846.0 35 406962.0 36 654318.0 37 1187489.0 38 1721901.0 39 724583.0 40 2652.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.40415316858832 20.73991079473452 14.65987811890978 23.196057917767384 2 15.368168107158617 20.71163688389369 40.203033179356915 23.71716182959078 3 17.648032727519784 25.666228901349776 31.19279065825084 25.492947712879598 4 12.370317320096587 17.47814071897217 38.71012638418477 31.441415576746472 5 12.377171209333834 37.97611925334079 35.985941598896595 13.66076793842878 6 30.20098015692603 38.7466728538681 17.356353284584387 13.695993704621486 7 25.931609008511032 33.35484781663723 22.792165473970552 17.92137770088118 8 24.545446749930658 36.73434050902312 20.444235124633785 18.27597761641243 9 24.46175314125748 16.644690451988843 21.924176452623392 36.96937995413029 10-11 23.42231336652962 27.29233136745704 29.067779197418524 20.217576068594813 12-13 24.100647499485273 24.852634805538983 29.41026536128518 21.636452333690563 14-15 21.353758485664336 26.842003326283955 27.071937589671847 24.732300598379865 16-17 20.346094049403938 30.224069990560892 27.83093276993311 21.59890319010206 18-19 20.206931596694957 28.56258264273545 30.747183087036646 20.483302673532943 20-21 21.264262665981214 27.785942825245673 30.54769214532511 20.402102363447998 22-23 20.97330664965736 27.6061520428645 30.630228695549505 20.790312611928634 24-25 20.844873884505 27.75128773751988 30.571868253353657 20.831970124621463 26-27 20.657504532186874 27.703969846930114 30.61929539551911 21.019230225363906 28-29 20.674054617760763 27.69939948299074 30.78564069109587 20.840905208152623 30-31 20.936046962717402 28.047298462211273 30.349813604739147 20.66684097033218 32-33 20.619971394028664 27.850931672921632 30.69512906121475 20.833967871834957 34-35 20.794560933647684 27.58665753977199 30.67555126218456 20.94323026439576 36-37 21.056344961113115 27.935678128113082 30.152295623096286 20.855681287677516 38-39 20.978840679489373 28.032123414596942 30.195572723955895 20.79346318195779 40-41 20.855074933747638 27.799631719241862 30.329931440215557 21.015361906794936 42-43 20.83858589767998 27.862635544606235 30.16418837976244 21.134590177951342 44-45 20.974360061037775 27.92465048683538 29.99841609165132 21.102573360475528 46-47 21.08043011008024 28.102806968593306 29.608523707141778 21.208239214184673 48-49 21.120983997085794 28.091749403825773 29.38935697285146 21.397909626236977 50-51 21.170400538183426 28.13231172588001 29.227922973280386 21.46936476265618 52-53 21.133626078572885 28.201702827769942 29.21193692804171 21.452734165615464 54-55 21.210868684410105 28.483191490379202 29.009493740292253 21.29644608491844 56-57 21.150725326458332 28.25724093793355 29.023777159237397 21.56825657637073 58-59 21.013322291339126 27.90901139521929 29.329752930432466 21.747913383009116 60-61 21.163467381472813 28.230961978788333 29.090164022285197 21.515406617453657 62-63 21.004728254215088 28.29585373867666 29.069516344406875 21.629901662701375 64-65 21.012527577251138 28.07412606797561 29.174420975638355 21.738925379134898 66-67 20.975192783954995 28.089152416820333 29.026612942324608 21.909041856900068 68-69 21.289830733194854 28.178980137980712 28.882224643785097 21.648964485039336 70-71 21.284866946185723 28.035333812161777 28.917409349588436 21.762389892064064 72-73 21.09029432217872 28.147494693744413 28.97657496971093 21.78563601436593 74-75 21.170410301991897 28.12752821125086 28.84104240221501 21.861019084542228 76-77 21.22090616788746 28.11266199061161 28.70862154659416 21.957810294906768 78-79 21.240206325060175 28.171779456759577 28.76047392724829 21.827540290931957 80-81 21.159148908100708 28.042035133720045 28.782877567931926 22.01593839024732 82-83 21.03403233743224 28.105685369660176 28.88781040280631 21.97247189010128 84-85 21.10046241991013 27.97756716764218 28.728576943582663 22.19339346886503 86-87 21.13301736719862 28.088562645421668 28.678375075125167 22.10004491225455 88-89 21.025763395363516 27.951105955373922 28.750222315743024 22.272908333519542 90-91 20.923023949407405 27.838328039359688 28.782593846721383 22.456054164511517 92-93 20.995311159861696 27.970729750940514 28.63951571024475 22.39444337895304 94-95 20.831926442076938 27.774585388631394 28.829731155758388 22.563757013533284 96-97 20.838995906786 27.767924446469532 28.83276035593366 22.560319290810806 98-99 20.797733694417154 27.845934492376774 28.73659746772986 22.61973434547621 100 20.916244685694743 27.7093536554818 28.668464033237157 22.7059376255863 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 892.0 1 636.5 2 646.0 3 931.5 4 624.0 5 421.0 6 1061.5 7 2754.5 8 4625.0 9 5343.0 10 4188.5 11 2765.0 12 2637.0 13 3049.5 14 3576.5 15 4104.0 16 4785.0 17 5573.0 18 6535.5 19 7731.0 20 9317.0 21 11726.0 22 15177.0 23 20241.5 24 27334.0 25 36383.0 26 48546.0 27 63348.5 28 80620.5 29 100778.5 30 120069.0 31 136580.0 32 150437.0 33 163548.5 34 177340.0 35 187822.5 36 196233.5 37 206828.5 38 216796.5 39 220930.0 40 221916.5 41 222743.5 42 221469.0 43 218803.0 44 214293.5 45 209201.0 46 204659.5 47 196830.5 48 187559.0 49 180761.0 50 175766.0 51 168755.0 52 156208.0 53 145346.0 54 136546.0 55 127745.5 56 118818.5 57 108077.5 58 98357.0 59 89388.5 60 77891.0 61 65209.5 62 55562.5 63 46592.5 64 37726.0 65 30478.0 66 24179.5 67 18460.0 68 14070.0 69 10798.0 70 8028.0 71 5883.5 72 4250.0 73 2942.5 74 2143.0 75 1526.0 76 996.5 77 658.5 78 428.0 79 263.0 80 142.0 81 73.0 82 42.5 83 24.0 84 12.0 85 4.5 86 2.5 87 2.5 88 1.5 89 1.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.035586296604361516 2 0.0 3 0.0 4 4.9471218634422864E-5 5 0.0 6 1.6490406211474288E-5 7 0.0 8 7.585586857278172E-4 9 0.0 10-11 0.0 12-13 0.04386448052252161 14-15 1.2367804658605717E-4 16-17 0.0 18-19 4.122601552868572E-5 20-21 7.42068279516343E-5 22-23 0.0 24-25 1.319232496917943E-4 26-27 1.0718764037458287E-4 28-29 3.957697490753829E-4 30-31 4.122601552868572E-5 32-33 2.226204838549029E-4 34-35 4.9471218634422864E-5 36-37 8.245203105737144E-5 38-39 3.2156292112374863E-4 40-41 1.4016845279753146E-4 42-43 5.029573894499657E-4 44-45 3.0507251491227437E-4 46-47 1.6490406211474288E-4 48-49 8.080299043622402E-4 50-51 4.6997657702701725E-4 52-53 7.008422639876573E-4 54-55 5.11202592555703E-4 56-57 5.771642174016001E-4 58-59 1.6490406211474288E-4 60-61 2.803369055950629E-4 62-63 8.987271385253486E-4 64-65 5.029573894499657E-4 66-67 0.0014841365590326859 68-69 0.0015830789963015316 70-71 4.205053583925944E-4 72-73 0.0010388955913228802 74-75 0.001022405185111406 76-77 7.585586857278172E-4 78-79 0.0033887784764579662 80-81 0.0025889937752014633 82-83 0.001253270872072046 84-85 0.002687936212470309 86-87 0.0014099297310810517 88-89 0.0020035843546941257 90-91 0.0026632006031530975 92-93 0.002621974587624412 94-95 0.0038010386317448236 96-97 0.0031826483988145375 98-99 0.0027374074311047316 100 0.0012367804658605715 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6064132.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.44675165138482 #Duplication Level Percentage of deduplicated Percentage of total 1 75.26830956680519 45.497248156042254 2 14.912822547408064 18.028633618886943 3 4.518236363209174 8.193381340474831 4 1.9166059903428747 4.634104252472488 5 0.9881613586184008 2.9865572117950743 6 0.5810646960974625 2.1074084027034425 7 0.37060704180986903 1.5681394271574882 8 0.25087082392569166 1.2131461112331658 9 0.18768175937357545 1.0210277428514534 >10 0.93925261713339 9.898259690992612 >50 0.04720262544743642 1.9385317658557382 >100 0.01839273540665584 1.8483021280651797 >500 4.095955529627239E-4 0.17763444610752013 >1k 3.0036196870602785E-4 0.290015829466584 >5k 2.730563348872836E-5 0.12918751895026592 >10k+ 5.461126697745672E-5 0.4684223569450041 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14400 0.23746184944522974 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13980 0.23053587883641058 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7827 0.12907040941720926 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.6490406211474288E-5 0.0 0.0 0.0 3 0.0 1.6490406211474288E-5 0.0 0.0 0.0 4 0.0 1.6490406211474288E-5 0.0 1.6490406211474288E-5 0.0 5 0.0 1.1543284348032002E-4 0.0 3.2980812422948576E-5 0.0 6 0.0 1.1543284348032002E-4 0.0 3.2980812422948576E-5 0.0 7 0.0 1.1543284348032002E-4 0.0 4.9471218634422864E-5 0.0 8 0.0 1.1543284348032002E-4 0.0 6.596162484589715E-5 0.0 9 0.0 1.1543284348032002E-4 0.0 9.894243726884573E-5 0.0 10-11 0.0 1.1543284348032002E-4 0.0 1.4016845279753143E-4 0.0 12-13 0.0 1.6490406211474288E-4 0.0 1.7314926522048E-4 0.0 14-15 0.0 2.3086568696064003E-4 0.0 2.556012962778515E-4 0.0 16-17 0.0 2.8858210870080005E-4 0.0 2.968273118065372E-4 0.0 18-19 0.0 2.968273118065372E-4 0.0 3.2156292112374863E-4 0.0 20-21 0.0 2.968273118065372E-4 0.0 4.0401495218112004E-4 0.0 22-23 0.0 3.133177180180115E-4 0.0 6.431258422474973E-4 0.0 24-25 0.0 3.380533273352229E-4 0.0 0.001146083231697463 0.0 26-27 0.0 3.462985304409601E-4 0.0 0.002069545979540023 0.0 28-29 0.0 3.710341397581715E-4 0.0 0.005219213565931613 0.0 30-31 0.0 3.7927934286390865E-4 0.0 0.015443265417045672 0.0 32-33 0.0 3.7927934286390865E-4 0.0 0.03895033947150227 0.0 34-35 0.0 3.7927934286390865E-4 0.0 0.07808207341133075 0.0 36-37 0.0 3.875245459696458E-4 0.0 0.1392037640341602 0.0 38-39 0.0 4.287505614983315E-4 0.0 0.21836595905234252 0.0 40-41 0.0 4.452409677098058E-4 0.0 0.29956471923764194 0.0 42-43 0.0 4.452409677098058E-4 0.0 0.3811097779533823 0.0 44-45 0.0 4.452409677098058E-4 0.0 0.47916997848991416 0.0 46-47 0.0 4.452409677098058E-4 0.0 0.5967630651839373 0.0 48-49 0.0 4.6173137392128006E-4 0.0 0.7194104613817773 0.0 50-51 0.0 4.6173137392128006E-4 0.0 0.8397904267255396 0.0 52-53 0.0 4.6173137392128006E-4 0.0 0.9561467329537021 0.0 54-55 0.0 4.6173137392128006E-4 0.0 1.08570360935415 0.0 56-57 0.0 4.6173137392128006E-4 0.0 1.235889983925152 0.0 58-59 0.0 4.782217801327544E-4 0.0 1.39794120576531 0.0 60-61 0.0 4.782217801327544E-4 0.0 1.5637192594092608 0.0 62-63 0.0 4.782217801327544E-4 0.0 1.7253005046723917 0.0 64-65 0.0 4.782217801327544E-4 0.0 1.8985157315177177 0.0 66-67 0.0 4.8646698323849156E-4 0.0 2.093044808391374 0.0 68-69 0.0 5.359382018729143E-4 0.0 2.3050784514585105 0.0 70-71 8.245203105737144E-6 5.441834049786515E-4 0.0 2.5301395154327118 0.0 72-73 1.6490406211474288E-5 5.524286080843886E-4 0.0 2.7575504622920475 0.0 74-75 1.6490406211474288E-5 5.606738111901258E-4 0.0 2.9859343431178607 0.0 76-77 1.6490406211474288E-5 5.606738111901258E-4 0.0 3.2290441566905206 0.0 78-79 1.6490406211474288E-5 5.606738111901258E-4 0.0 3.5015646097413446 0.0 80-81 1.6490406211474288E-5 5.606738111901258E-4 0.0 3.7929500874980953 0.0 82-83 1.6490406211474288E-5 6.018998267188115E-4 0.0 4.091871680893489 0.0 84-85 1.6490406211474288E-5 6.101450298245486E-4 0.0 4.386019301690663 0.0 86-87 1.6490406211474288E-5 6.26635436036023E-4 0.0 4.7033854144335905 0.0 88 1.6490406211474288E-5 6.431258422474973E-4 0.0 4.956405962139346 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10100 0.0 58.1818 1 GTATCAA 18010 0.0 39.571636 1 TCAACGC 21790 0.0 32.092575 4 ATCAACG 21925 0.0 31.959274 3 CAACGCA 22540 0.0 31.04557 5 TATCAAC 23310 0.0 30.120846 2 AACGCAG 23315 0.0 30.11931 6 GTGGTAT 4325 0.0 28.369581 1 TGGTATC 4330 0.0 26.157003 2 ACGCAGA 27085 0.0 25.579859 7 CGCAGAG 27220 0.0 25.297478 8 GTACATG 15915 0.0 24.93069 1 TACATGG 16025 0.0 23.519884 2 GCAGAGT 29920 0.0 22.668768 9 ACATGGG 16515 0.0 22.167551 3 GAGTACT 21555 0.0 20.972229 12-13 CAGAGTA 29355 0.0 20.757717 10-11 AGAGTAC 27460 0.0 20.365993 10-11 CCGGTCG 535 0.0 20.220798 9 CATGGGG 11665 0.0 19.741047 4 >>END_MODULE