FastQCFastQC Report
Wed 25 May 2016
SRR2049354_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049354_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6360488
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63050.046.8309251
GTATCAA105300.034.1579061
TCAACGC109900.031.7407054
ATCAACG113150.030.9121153
CAACGCA115850.030.03035
AACGCAG122350.028.6593696
TATCAAC129850.027.0813272
GTACATG181150.025.435831
TACATGG181500.023.9075682
ACGCAGA144250.023.7214097
CGCAGAG149400.022.809328
ACATGGG185250.022.0785923
GTGGTAT35500.019.8664861
GCAGAGT169700.019.305279
CATGGGG128150.019.002974
GAGTACA137400.017.8282681
CAGAGTA161400.017.69162410-11
AGAGTAC145350.016.52459510-11
TGGTATC38950.016.0529382
AGTACAT141750.016.052132
Produced by FastQC (version 0.11.5)