##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049354_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6360488
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.890312504323568	31.0	31.0	34.0	28.0	34.0
2	31.080054549273576	31.0	31.0	34.0	28.0	34.0
3	31.136701303422	31.0	31.0	34.0	28.0	34.0
4	34.743133388507296	37.0	35.0	37.0	32.0	37.0
5	34.77112086368216	37.0	35.0	37.0	32.0	37.0
6	34.7531407967439	37.0	35.0	37.0	32.0	37.0
7	34.7095290487145	37.0	35.0	37.0	32.0	37.0
8	34.676631415702694	37.0	35.0	37.0	32.0	37.0
9	36.26656555283179	39.0	37.0	39.0	32.0	39.0
10-11	36.237152793936566	39.0	37.0	39.0	32.0	39.0
12-13	36.18585169880047	39.0	37.0	39.0	32.0	39.0
14-15	37.4081807087758	40.0	38.0	41.0	32.0	41.0
16-17	37.377157067193586	40.0	38.0	41.0	32.0	41.0
18-19	37.40449710776909	40.0	38.0	41.0	32.0	41.0
20-21	37.479514622148486	40.0	38.0	41.0	32.0	41.0
22-23	37.42392517681033	40.0	38.0	41.0	32.0	41.0
24-25	37.427606419507434	40.0	38.0	41.0	32.0	41.0
26-27	37.414886247721874	40.0	38.0	41.0	32.0	41.0
28-29	37.34945353249625	40.0	38.0	41.0	32.0	41.0
30-31	37.27812763737626	40.0	38.0	41.0	32.0	41.0
32-33	37.21848504391487	40.0	38.0	41.0	31.0	41.0
34-35	37.106270069214816	40.0	37.0	41.0	31.0	41.0
36-37	37.01986357021663	40.0	37.0	41.0	31.0	41.0
38-39	36.91904190370299	40.0	37.0	41.0	30.5	41.0
40-41	36.815652352461	40.0	37.0	41.0	30.0	41.0
42-43	36.674945381549335	40.0	37.0	41.0	30.0	41.0
44-45	36.55570225114803	40.0	37.0	41.0	30.0	41.0
46-47	36.43992984500561	40.0	36.0	41.0	30.0	41.0
48-49	36.31612134163291	40.0	36.0	41.0	30.0	41.0
50-51	36.179685505263116	40.0	36.0	41.0	29.5	41.0
52-53	36.03680063542294	39.0	35.5	41.0	29.0	41.0
54-55	35.897069768860504	39.0	35.0	41.0	29.0	41.0
56-57	35.770706744513944	39.0	35.0	41.0	28.5	41.0
58-59	35.56745213574807	39.0	35.0	41.0	28.0	41.0
60-61	35.35217832342424	39.0	35.0	40.5	28.0	41.0
62-63	35.14113696936462	38.0	35.0	40.0	28.0	41.0
64-65	34.890195532166715	38.0	34.0	40.0	28.0	41.0
66-67	34.61481178802633	37.5	34.0	40.0	27.0	41.0
68-69	34.309840927299916	37.0	34.0	40.0	26.0	41.0
70-71	34.00167919505547	36.5	34.0	39.0	26.0	41.0
72-73	33.62698962721099	36.0	34.0	39.0	26.0	41.0
74-75	33.23231464315317	35.5	33.0	39.0	25.5	40.0
76-77	32.83459791135523	35.0	33.0	37.5	24.5	40.0
78-79	32.43460085138122	35.0	33.0	37.0	23.5	39.0
80-81	31.96777212691856	35.0	33.0	37.0	21.0	39.0
82-83	31.535213414442413	35.0	32.5	36.0	19.0	38.5
84-85	31.132060936204894	35.0	32.0	36.0	14.5	37.0
86-87	30.696278335876116	35.0	32.0	35.5	4.5	37.0
88-89	30.311553138689987	35.0	32.0	35.0	2.0	36.5
90-91	29.961655850934708	35.0	31.0	35.0	2.0	36.0
92-93	29.583973195138487	35.0	31.0	35.0	2.0	36.0
94-95	29.248169715908592	35.0	31.0	35.0	2.0	36.0
96-97	28.870918473551086	34.0	30.5	35.0	2.0	35.0
98-99	28.548249049444003	34.0	30.0	35.0	2.0	35.0
100	28.361926003162022	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.41575465888078256
1101	2	0.4263771174072879
1101	3	0.4026216002175751
1101	4	0.41458873047212563
1101	5	0.39099626904771156
1101	6	0.4692577293108755
1101	7	0.4907151184543963
1101	8	0.49524737556019716
1101	9	0.6798455197404394
1101	10-11	0.6253876958268876
1101	12-13	0.6065129603813801
1101	14-15	0.6531554735389165
1101	16-17	0.7158884883598091
1101	18-19	0.6597723496047934
1101	20-21	0.6673654092966359
1101	22-23	0.6719376780989492
1101	24-25	0.689803379144081
1101	26-27	0.6310101138388262
1101	28-29	0.6629125889705207
1101	30-31	0.6703193219086643
1101	32-33	0.6780814240162485
1101	34-35	0.7067633432987179
1101	36-37	0.6542592893085128
1101	38-39	0.7167736820383013
1101	40-41	0.7370176537160731
1101	42-43	0.6847627321341321
1101	44-45	0.6477384352496287
1101	46-47	0.6303696146275257
1101	48-49	0.5738368756665935
1101	50-51	0.6051457475040962
1101	52-53	0.6155971394865034
1101	54-55	0.6138798781863457
1101	56-57	0.6089722470818586
1101	58-59	0.7026466249915373
1101	60-61	0.6881542902512976
1101	62-63	0.6526032953131349
1101	64-65	0.6318154805710492
1101	66-67	0.6621220896866049
1101	68-69	0.646360971732193
1101	70-71	0.5915037889913677
1101	72-73	0.731436224548176
1101	74-75	0.6542933576523353
1101	76-77	0.6781294727497524
1101	78-79	0.6205638824608357
1101	80-81	0.6475628205235822
1101	82-83	0.6467109953818024
1101	84-85	0.6083072770958466
1101	86-87	0.6966072522201223
1101	88-89	0.7205893233286247
1101	90-91	0.7263131503135796
1101	92-93	0.7354667113503162
1101	94-95	0.7804825859569817
1101	96-97	0.8046406288550934
1101	98-99	0.8209508162973016
1101	100	0.8919392158925099
1102	1	0.2552395984172904
1102	2	0.3285971274481554
1102	3	0.33930976744001384
1102	4	0.3723967232050853
1102	5	0.28175942544012145
1102	6	0.3737394534187004
1102	7	0.5033331574290898
1102	8	0.4753084645075205
1102	9	0.5709509975548173
1102	10-11	0.5102035159878753
1102	12-13	0.5682748599861327
1102	14-15	0.6858045628086273
1102	16-17	0.5638669239991785
1102	18-19	0.6331203391643001
1102	20-21	0.5602321843475906
1102	22-23	0.5484752385876277
1102	24-25	0.6237597962330312
1102	26-27	0.5915545266327058
1102	28-29	0.6172524522266443
1102	30-31	0.6104703714984723
1102	32-33	0.6570877390174417
1102	34-35	0.625072250288035
1102	36-37	0.5975609657809784
1102	38-39	0.6245937221346125
1102	40-41	0.6353285147289611
1102	42-43	0.6106964237820733
1102	44-45	0.6081163572599522
1102	46-47	0.570671676027942
1102	48-49	0.5809349030052928
1102	50-51	0.6496692029229791
1102	52-53	0.614913546064443
1102	54-55	0.5869229582144158
1102	56-57	0.5473382300291689
1102	58-59	0.7275570673270693
1102	60-61	0.5764468976197534
1102	62-63	0.5960704131472596
1102	64-65	0.6163768578995032
1102	66-67	0.6787853352378903
1102	68-69	0.6902111705346741
1102	70-71	0.7118343862719385
1102	72-73	0.6583917907658545
1102	74-75	0.641898215888034
1102	76-77	0.6928201724371092
1102	78-79	0.6677468877868549
1102	80-81	0.6115544792985972
1102	82-83	0.5633540500558425
1102	84-85	0.5627360263864389
1102	86-87	0.5685303996966375
1102	88-89	0.5490457832339892
1102	90-91	0.6195915086315473
1102	92-93	0.5750672447084995
1102	94-95	0.5908398763328577
1102	96-97	0.5661244001751449
1102	98-99	0.6381462446797137
1102	100	0.6383823053234039
1103	1	0.2168906618976223
1103	2	0.31135355349726623
1103	3	0.29991788109690987
1103	4	0.30481439586666426
1103	5	0.2829686723810738
1103	6	0.3596308880906207
1103	7	0.3790989453899982
1103	8	0.3711510365696
1103	9	0.47497087108688874
1103	10-11	0.4601172878528672
1103	12-13	0.4669403769443292
1103	14-15	0.4979134566248291
1103	16-17	0.5026603460243066
1103	18-19	0.47354798965292844
1103	20-21	0.4343062584216639
1103	22-23	0.4157721918601922
1103	24-25	0.48277268939226303
1103	26-27	0.4706914240622879
1103	28-29	0.4702632512740337
1103	30-31	0.4886803164767102
1103	32-33	0.5302133036064163
1103	34-35	0.5256532636739237
1103	36-37	0.5136928127055072
1103	38-39	0.5627465922996748
1103	40-41	0.5564975218735739
1103	42-43	0.5411554859484511
1103	44-45	0.4443034312519032
1103	46-47	0.4360254166865616
1103	48-49	0.4592967737452085
1103	50-51	0.38913807867233885
1103	52-53	0.42781183792615707
1103	54-55	0.4199627052785502
1103	56-57	0.36102489341094923
1103	58-59	0.43759853895389966
1103	60-61	0.3504953085584077
1103	62-63	0.35850060941892536
1103	64-65	0.42003231490862447
1103	66-67	0.3953863042778849
1103	68-69	0.4347089943007916
1103	70-71	0.3872090680490672
1103	72-73	0.385305234142713
1103	74-75	0.38457293051396135
1103	76-77	0.4745172242561111
1103	78-79	0.33320841361423703
1103	80-81	0.3413791487574045
1103	82-83	0.33669107888067984
1103	84-85	0.39022535052210117
1103	86-87	0.41693107309731303
1103	88-89	0.3387836086425473
1103	90-91	0.3577905724403685
1103	92-93	0.30346299078717465
1103	94-95	0.3276716082865434
1103	96-97	0.24689552606822573
1103	98-99	0.27243322012522597
1103	100	0.3209026305266569
1104	1	0.12241079432046931
1104	2	0.13586484556150324
1104	3	0.12895291589345348
1104	4	0.14521052117264333
1104	5	0.22074232768970603
1104	6	0.21770643367198517
1104	7	0.24855998325309514
1104	8	0.2891928327706097
1104	9	0.33941374211871533
1104	10-11	0.3047024721342879
1104	12-13	0.268131188236012
1104	14-15	0.3985954093046047
1104	16-17	0.4096635345866062
1104	18-19	0.35604668310134
1104	20-21	0.3661351040064815
1104	22-23	0.3901691366430171
1104	24-25	0.38657934185339116
1104	26-27	0.3422680907801947
1104	28-29	0.37923568207483527
1104	30-31	0.37506739391294985
1104	32-33	0.3129829383868241
1104	34-35	0.32438859859304614
1104	36-37	0.30789081099161564
1104	38-39	0.4376499939451648
1104	40-41	0.41205363250006855
1104	42-43	0.3769234644825801
1104	44-45	0.32102188283456456
1104	46-47	0.239670623563633
1104	48-49	0.23809230610734033
1104	50-51	0.2785009714544131
1104	52-53	0.2958740009607368
1104	54-55	0.349843924368038
1104	56-57	0.3810664821008274
1104	58-59	0.43523466529744326
1104	60-61	0.38504521594695973
1104	62-63	0.37062264614070983
1104	64-65	0.4181477103134057
1104	66-67	0.3958355995085867
1104	68-69	0.4191981374335185
1104	70-71	0.39466923743963633
1104	72-73	0.4039279174153947
1104	74-75	0.39886110487712045
1104	76-77	0.3886747194430029
1104	78-79	0.3570223097510663
1104	80-81	0.4369265698551459
1104	82-83	0.4312723723283227
1104	84-85	0.44763834955755755
1104	86-87	0.5285962971132641
1104	88-89	0.4578066875313631
1104	90-91	0.5162307756091415
1104	92-93	0.5166125198521812
1104	94-95	0.6060346096276774
1104	96-97	0.6267360114905678
1104	98-99	0.659420975046892
1104	100	0.6379314169323713
1105	1	0.20665019585660005
1105	2	0.22525436783008246
1105	3	0.2463690404136969
1105	4	0.26682776275357867
1105	5	0.277326083487651
1105	6	0.26508903358116953
1105	7	0.25708250444636604
1105	8	0.31802707026500343
1105	9	0.37945934454305075
1105	10-11	0.3249464848461372
1105	12-13	0.32034919025323916
1105	14-15	0.3719725673458072
1105	16-17	0.3662233939924491
1105	18-19	0.35022565902701785
1105	20-21	0.3327103496054349
1105	22-23	0.373899302352072
1105	24-25	0.3573236199665075
1105	26-27	0.355728394561865
1105	28-29	0.3676494264344896
1105	30-31	0.3914855685552041
1105	32-33	0.4018299126296512
1105	34-35	0.4575442059599695
1105	36-37	0.3981828231900266
1105	38-39	0.35892865476965596
1105	40-41	0.3831270641430251
1105	42-43	0.40569489757633903
1105	44-45	0.3450069285466171
1105	46-47	0.3317184353050635
1105	48-49	0.3342571207512748
1105	50-51	0.41521931675744383
1105	52-53	0.3960676730819017
1105	54-55	0.3822375423126374
1105	56-57	0.3894171091512888
1105	58-59	0.3577630360534201
1105	60-61	0.394372237587433
1105	62-63	0.3421027960901455
1105	64-65	0.368068249813426
1105	66-67	0.38285075684449055
1105	68-69	0.40251876980114076
1105	70-71	0.350205811652863
1105	72-73	0.3117797294071494
1105	74-75	0.39139672277786275
1105	76-77	0.41039558628648365
1105	78-79	0.32666192931822735
1105	80-81	0.3432130339793744
1105	82-83	0.29755214203676417
1105	84-85	0.3809749528233226
1105	86-87	0.3765273606186277
1105	88-89	0.34593929383576594
1105	90-91	0.4065188421906001
1105	92-93	0.3788726994297136
1105	94-95	0.3649979036274189
1105	96-97	0.3746079135817091
1105	98-99	0.42250209419059104
1105	100	0.44185355432160733
1106	1	0.18079006619333882
1106	2	0.17651301784914963
1106	3	0.21122166715223756
1106	4	0.21753890680579246
1106	5	0.21366749730267287
1106	6	0.24591377569047523
1106	7	0.24079544199003777
1106	8	0.2462021203979674
1106	9	0.2665673557978252
1106	10-11	0.24712246169517726
1106	12-13	0.3085967235288507
1106	14-15	0.40144819848553936
1106	16-17	0.4313853366911289
1106	18-19	0.4240482341428802
1106	20-21	0.35995227166252164
1106	22-23	0.3835997077821034
1106	24-25	0.4126575385556066
1106	26-27	0.3181183002856045
1106	28-29	0.35150788787684206
1106	30-31	0.3517579731480467
1106	32-33	0.35929165974570765
1106	34-35	0.3872583020637066
1106	36-37	0.34277105319202406
1106	38-39	0.35982204601705803
1106	40-41	0.39320741106434554
1106	42-43	0.38354525613714685
1106	44-45	0.2939910878168419
1106	46-47	0.272911630089979
1106	48-49	0.2785150720488616
1106	50-51	0.30020807265376703
1106	52-53	0.29096557098938547
1106	54-55	0.27562263617370775
1106	56-57	0.2729741954002307
1106	58-59	0.33965402406406753
1106	60-61	0.30395930561989815
1106	62-63	0.22475329314144687
1106	64-65	0.28652509378098756
1106	66-67	0.2988592875595799
1106	68-69	0.2913001381668252
1106	70-71	0.30092399461186403
1106	72-73	0.3631996528788335
1106	74-75	0.3619860255532501
1106	76-77	0.4260193802550418
1106	78-79	0.33444893991761404
1106	80-81	0.40336374797198715
1106	82-83	0.41171586326921883
1106	84-85	0.44275618008091655
1106	86-87	0.4254801142664597
1106	88-89	0.39696531348465314
1106	90-91	0.4676744316160075
1106	92-93	0.45914147238658387
1106	94-95	0.4608180852950028
1106	96-97	0.4600242665679275
1106	98-99	0.47370293718766376
1106	100	0.3747657337310244
1107	1	0.17052637839722706
1107	2	0.16402469562552824
1107	3	0.1802941400333573
1107	4	0.19448490107610894
1107	5	0.16070353477368826
1107	6	0.19963915327591764
1107	7	0.25233552610100674
1107	8	0.3121139420092973
1107	9	0.2945854727628614
1107	10-11	0.2841831970289306
1107	12-13	0.2753617265537258
1107	14-15	0.2823439705990154
1107	16-17	0.3238898093662854
1107	18-19	0.3022260822570644
1107	20-21	0.3078463405966829
1107	22-23	0.3202830472955114
1107	24-25	0.3410857571270469
1107	26-27	0.384935046075384
1107	28-29	0.44848806431395616
1107	30-31	0.4278266692933954
1107	32-33	0.40829361781969453
1107	34-35	0.444721091553717
1107	36-37	0.44673354372027774
1107	38-39	0.4670501369061313
1107	40-41	0.5204202435264307
1107	42-43	0.4957159268188889
1107	44-45	0.4474909487785297
1107	46-47	0.4777165496640805
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2214	98-99	-0.23713732984968772
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2215	26-27	-0.5126378251978139
2215	28-29	-0.48349445295341553
2215	30-31	-0.5103641491679056
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2216	1	-0.9945857499655695
2216	2	-1.026926164985582
2216	3	-1.0342466253413605
2216	4	-1.2074629243887074
2216	5	-1.1922127878288222
2216	6	-2.5120948009131183
2216	7	-2.4203678465156173
2216	8	-3.3302015420064635
2216	9	-2.6858884336525435
2216	10-11	-1.9013527360453963
2216	12-13	-1.6812443550101008
2216	14-15	-1.8625179927762332
2216	16-17	-2.281899184583729
2216	18-19	-1.8959782825561433
2216	20-21	-1.7240723797728137
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2216	24-25	-2.0608024471114277
2216	26-27	-2.1305195281573717
2216	28-29	-2.575619579822849
2216	30-31	-2.133709008829207
2216	32-33	-2.2712100241145237
2216	34-35	-3.3019930903912496
2216	36-37	-2.2793362065846097
2216	38-39	-2.186610712858169
2216	40-41	-2.706567474926807
2216	42-43	-2.4420806089323435
2216	44-45	-2.1869551863535506
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2216	50-51	-2.5239057820067075
2216	52-53	-2.887192682314627
2216	54-55	-2.2462873144437125
2216	56-57	-2.205638971157221
2216	58-59	-3.2395058728707653
2216	60-61	-4.01287679208928
2216	62-63	-3.018124868961273
2216	64-65	-3.037044945759895
2216	66-67	-2.70626228245019
2216	68-69	-3.012026699270322
2216	70-71	-2.768913599114299
2216	72-73	-3.2445197540597874
2216	74-75	-3.7086825516098756
2216	76-77	-4.354900071422755
2216	78-79	-3.278284416394591
2216	80-81	-3.122658755080309
2216	82-83	-3.3202121750574705
2216	84-85	-3.331069419788811
2216	86-87	-2.943179804966629
2216	88-89	-2.8007905000493665
2216	90-91	-2.972079177620639
2216	92-93	-2.9926090203777846
2216	94-95	-2.861813914136288
2216	96-97	-3.068092565781594
2216	98-99	-2.8177313065988017
2216	100	-2.4352544353412036
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	116862.0
3	14869.0
4	9445.0
5	8282.0
6	7780.0
7	8597.0
8	10663.0
9	12037.0
10	13156.0
11	14607.0
12	16181.0
13	17737.0
14	19649.0
15	19948.0
16	19959.0
17	21000.0
18	20180.0
19	18612.0
20	18792.0
21	19863.0
22	21909.0
23	26081.0
24	31912.0
25	40110.0
26	50261.0
27	60927.0
28	77405.0
29	100499.0
30	124840.0
31	150244.0
32	185404.0
33	236380.0
34	315738.0
35	442339.0
36	710863.0
37	1218831.0
38	1576935.0
39	579780.0
40	1811.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.17918696737425	20.467343347309455	13.921781412348984	23.431688272967314
2	15.75569038062961	20.43299285921346	40.03467919152562	23.776637568631312
3	18.44625085920923	25.259162670131293	30.362322829877357	25.93226364078212
4	12.900569523754696	16.77132395122107	38.65602964222734	31.672076882796894
5	13.229624316327445	37.71130731877663	35.63888875780413	13.42017960709179
6	30.937827619917247	37.82736824223091	17.666563637207318	13.568240500644524
7	26.978987483216788	32.59986875434917	22.557956342889643	17.863187419544403
8	25.069059404772574	36.193309707954455	20.63794814685485	18.099682740418118
9	24.91535679754321	16.823144192379193	21.720881047456977	36.54061796262062
10-11	23.93948581344841	26.974294604773007	28.96288527715417	20.123334304624407
12-13	24.214488091750443	24.583652675890736	28.955672172663228	22.246187059695593
14-15	21.726638666485883	26.84589949191343	26.032319225969385	25.3951426156313
16-17	21.32100079649993	30.123024950706345	26.811046434813463	21.744927817980262
18-19	21.192122980224873	29.145940512999584	28.731885382984085	20.930051123791458
20-21	22.285779943392612	28.379800053494602	28.282232563743477	21.05218743936931
22-23	22.009441963414986	28.22918672148001	28.263043965184213	21.498327349920785
24-25	21.821432941761948	28.365888085221908	28.176860493610846	21.6358184794053
26-27	21.498232287385914	28.368439202757877	28.31862631565909	21.81470219419712
28-29	21.57793766270453	28.369174271406926	28.450028130945977	21.60285993494257
30-31	21.83611838131669	28.67788340547614	28.00140300986511	21.484595203342057
32-33	21.548974043753667	28.450443991735476	28.32142875118595	21.679153213324913
34-35	21.730241636922937	28.072429341519626	28.416959844096585	21.78036917746085
36-37	21.88452792903628	28.253574696132546	28.079218264117394	21.78267911071378
38-39	21.649405407658392	28.233264691992794	28.352092722038748	21.76523717831007
40-41	21.561162235113017	27.902438134382784	28.63480014401088	21.901599486493318
42-43	21.274506689047374	27.926363855991703	28.718910903298216	22.080218551662696
44-45	21.2901149658863	28.022283040414337	28.672439667662246	22.015162326037117
46-47	21.240329512217983	28.193612334940337	28.54037996830452	22.02567818453716
48-49	21.09912253839612	28.066471856947445	28.561360264447984	22.27304534020845
50-51	21.1044149892677	28.007879634640886	28.460048736148945	22.427656639942473
52-53	21.017216750620253	27.93211036764153	28.618479310235273	22.432193571502946
54-55	21.092524538234507	28.14547490655378	28.456023850511215	22.3059767047005
56-57	21.161823474608603	27.95380532249793	28.332931087266793	22.551440115626672
58-59	21.120486645244238	27.579756678950705	28.45164544044935	22.848111235355702
60-61	21.270867345494352	27.74493474383534	28.184088757191706	22.800109153478598
62-63	21.24702402531865	27.719357074652866	28.111878246008455	22.921740654020027
64-65	21.369232934818765	27.56686449534813	28.0640574880135	22.999845081819604
66-67	21.38066533157663	27.746522047465895	27.782468190690867	23.090344430266605
68-69	21.773225766066044	27.933713511714565	27.55204096590348	22.74101975631591
70-71	21.855445561528548	27.832155271535896	27.562540977102522	22.74985818983303
72-73	21.853433568381938	27.94518438703099	27.528933752121375	22.672448292465692
74-75	22.03649246146194	27.98496420939226	27.45815320070854	22.520390128437263
76-77	22.104965071749753	28.050360284207297	27.313725763122363	22.530948880920583
78-79	22.228616532009998	28.110939404932317	27.40218023245527	22.258263830602413
80-81	22.375302273093663	27.95878469505314	27.339729399228542	22.326183632624655
82-83	22.31058531362589	27.993971538205358	27.449499050238096	22.245944097930657
84-85	22.416112438727705	27.853307925299625	27.363357635165507	22.36722200080716
86-87	22.674462954082443	27.83382029984261	27.27957969628829	22.212137049786655
88-89	22.776491519648292	27.618171410425617	27.327742966541237	22.27759410338485
90-91	23.098047286421977	27.46376080459912	27.15584943909628	22.28234246988262
92-93	23.365388721285786	27.482783994204567	26.95069186839264	22.20113541611701
94-95	23.341399777006295	27.175458676095587	27.08181327320567	22.401328273692453
96-97	23.47132798615452	27.084533450892444	26.990570534839463	22.453568028113565
98-99	23.58267950509458	27.112613057362893	26.87583877211937	22.428868665423156
100	23.680981710837283	27.067592926832027	26.8855943128892	22.36583104944149
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1594.0
1	1267.5
2	1101.0
3	1400.0
4	1428.5
5	1224.5
6	1042.0
7	908.5
8	883.5
9	888.5
10	837.0
11	808.0
12	824.5
13	883.5
14	990.0
15	1180.0
16	1469.0
17	1878.5
18	2492.5
19	3436.0
20	4914.5
21	7058.5
22	10228.0
23	14659.5
24	20806.0
25	28844.0
26	39367.0
27	52774.5
28	68071.0
29	84903.5
30	101152.5
31	115830.5
32	129504.0
33	142517.5
34	157048.0
35	171548.5
36	184892.5
37	198410.5
38	210780.5
39	219319.0
40	225726.0
41	231831.0
42	235738.5
43	238264.5
44	237286.5
45	234310.5
46	231557.5
47	224974.0
48	217353.0
49	212546.0
50	207810.5
51	199174.0
52	187308.5
53	177126.5
54	168992.0
55	159177.0
56	145304.5
57	130009.5
58	115897.0
59	103014.5
60	89899.0
61	75997.0
62	64012.5
63	53658.0
64	43945.5
65	35636.0
66	28866.0
67	22857.0
68	17699.0
69	13656.0
70	10552.5
71	8248.0
72	6282.0
73	4468.5
74	3196.5
75	2418.0
76	1790.0
77	1262.5
78	905.0
79	674.0
80	496.5
81	357.5
82	270.5
83	218.0
84	164.0
85	127.0
86	108.5
87	82.0
88	65.0
89	55.5
90	54.5
91	49.5
92	44.5
93	42.0
94	36.5
95	31.5
96	42.0
97	51.5
98	53.5
99	68.5
100	157.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.020580181897992735
2	0.04378594849954909
3	0.045562541742080166
4	0.04828245883020297
5	0.051536926097494405
6	0.04847112359932131
7	0.04628575669036715
8	0.04587698302394408
9	0.04589270508803727
10-11	0.046702391388836834
12-13	0.04756710491396257
14-15	0.04575120651119851
16-17	0.042009355257018016
18-19	0.04528740562044925
20-21	0.04461135686444185
22-23	0.04578265063938491
24-25	0.04222946415432275
26-27	0.042378823763208104
28-29	0.04277973639758459
30-31	0.04288979084623695
32-33	0.04263823782074583
34-35	0.042536044404140064
36-37	0.03985543247625025
38-39	0.04025634511062673
40-41	0.04142763888556979
42-43	0.0413804726932902
44-45	0.040224900982440345
46-47	0.03905360720749729
48-49	0.03913221752796327
50-51	0.04054720329635085
52-53	0.0410345872832399
54-55	0.040138429629927766
56-57	0.038353975355350095
58-59	0.039454519841873766
60-61	0.03880205418200616
62-63	0.03910077339977687
64-65	0.03605069296569697
66-67	0.03718268158040704
68-69	0.03804739510553278
70-71	0.039564574290526136
72-73	0.03615288638230274
74-75	0.03656166004872582
76-77	0.03745781770203795
78-79	0.03780370311208825
80-81	0.03793734065688041
82-83	0.036404439407793865
84-85	0.037638621439109704
86-87	0.03803167304143958
88-89	0.03879419314995956
90-91	0.03914007856000986
92-93	0.03739492944566518
94-95	0.03869199973335379
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6360488.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.60426683609628
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.26922441652798	48.37383143590523
2	13.370652608961878	16.741198074083925
3	4.270342254829284	8.020249380083687
4	1.818141293354381	4.552936106995315
5	0.9671920385060648	3.0275174230190793
6	0.555016644399433	2.0847846062674114
7	0.37486807316284354	1.6427838616433943
8	0.2490221076034486	1.24718771779947
9	0.18538261553587143	1.0445168456803027
>10	0.8787666241997864	9.59792449410792
>50	0.04464858980536759	1.8900330241500514
>100	0.016491428434238963	1.6408737570118304
>500	1.7591868571855828E-4	0.06312137940098825
>1k	7.538599364365447E-5	0.07304189385140475
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.5722064093195364E-5	0.0	1.5722064093195364E-5	0.0
3	0.0	1.5722064093195364E-5	0.0	3.144412818639073E-5	0.0
4	0.0	1.5722064093195364E-5	0.0	4.7166192279586095E-5	0.0
5	0.0	1.5722064093195364E-5	0.0	7.861032046597683E-5	0.0
6	0.0	1.5722064093195364E-5	0.0	9.433238455917219E-5	0.0
7	0.0	1.5722064093195364E-5	0.0	1.1005444865236755E-4	0.0
8	0.0	1.5722064093195364E-5	0.0	1.1005444865236755E-4	0.0
9	0.0	1.5722064093195364E-5	0.0	1.8866476911834438E-4	0.0
10-11	0.0	1.5722064093195364E-5	0.0	2.3583096139793047E-4	0.0
12-13	0.0	1.5722064093195364E-5	0.0	3.065802498173096E-4	0.0
14-15	0.0	1.5722064093195364E-5	0.0	3.3016334595710266E-4	0.0
16-17	0.0	1.5722064093195364E-5	0.0	3.7732953823668876E-4	0.0
18-19	0.0	1.5722064093195364E-5	0.0	5.18828115075447E-4	0.0
20-21	0.0	1.5722064093195364E-5	0.0	7.625201085199752E-4	0.0
22-23	0.0	1.5722064093195364E-5	0.0	0.0022403941332803396	0.0
24-25	0.0	3.144412818639073E-5	0.0	0.004960311221403137	0.0
26-27	0.0	3.144412818639073E-5	0.0	0.010557366038580688	0.0
28-29	0.0	3.144412818639073E-5	0.0	0.03363735612739148	0.0
30-31	0.0	3.144412818639073E-5	0.0	0.10235063724670182	0.0
32-33	0.0	3.144412818639073E-5	0.0	0.2555307077067043	0.0
34-35	0.0	3.144412818639073E-5	0.0	0.5194098314468952	0.0
36-37	0.0	3.144412818639073E-5	0.0	0.928277830254534	0.0
38-39	0.0	3.144412818639073E-5	0.0	1.447341776291379	0.0
40-41	0.0	4.7166192279586095E-5	0.0	1.950636492042749	1.5722064093195364E-5
42-43	0.0	4.7166192279586095E-5	0.0	2.4376352883615215	1.5722064093195364E-5
44-45	0.0	4.7166192279586095E-5	0.0	3.006561760669936	1.5722064093195364E-5
46-47	0.0	4.7166192279586095E-5	0.0	3.660756847587795	1.5722064093195364E-5
48-49	0.0	6.288825637278146E-5	0.0	4.303514132877854	1.5722064093195364E-5
50-51	0.0	6.288825637278146E-5	0.0	4.918915026645754	1.5722064093195364E-5
52-53	0.0	6.288825637278146E-5	0.0	5.490647887394804	1.5722064093195364E-5
54-55	0.0	6.288825637278146E-5	0.0	6.096308962456969	1.5722064093195364E-5
56-57	0.0	6.288825637278146E-5	0.0	6.772247664015717	1.5722064093195364E-5
58-59	0.0	7.074928841937914E-5	0.0	7.467846806723006	1.5722064093195364E-5
60-61	0.0	9.433238455917219E-5	0.0	8.147071419677232	1.5722064093195364E-5
62-63	0.0	9.433238455917219E-5	0.0	8.776197675398492	1.5722064093195364E-5
64-65	1.5722064093195364E-5	9.433238455917219E-5	0.0	9.416376542177268	1.5722064093195364E-5
66-67	1.5722064093195364E-5	9.433238455917219E-5	0.0	10.106472962451939	1.5722064093195364E-5
68-69	1.5722064093195364E-5	1.0219341660576986E-4	0.0	10.832989544198496	1.5722064093195364E-5
70-71	1.5722064093195364E-5	1.1005444865236755E-4	0.0	11.559042325054303	1.5722064093195364E-5
72-73	1.5722064093195364E-5	1.1791548069896524E-4	1.5722064093195364E-5	12.253878947653075	1.5722064093195364E-5
74-75	1.5722064093195364E-5	1.257765127455629E-4	1.5722064093195364E-5	12.930533003128062	1.5722064093195364E-5
76-77	1.5722064093195364E-5	1.257765127455629E-4	1.5722064093195364E-5	13.625008018252688	1.5722064093195364E-5
78-79	1.5722064093195364E-5	1.257765127455629E-4	1.5722064093195364E-5	14.36717591480402	1.5722064093195364E-5
80-81	1.5722064093195364E-5	1.257765127455629E-4	1.5722064093195364E-5	15.122683982738431	1.5722064093195364E-5
82-83	1.5722064093195364E-5	1.257765127455629E-4	1.5722064093195364E-5	15.851016462887753	1.5722064093195364E-5
84-85	1.5722064093195364E-5	1.257765127455629E-4	1.5722064093195364E-5	16.546301164313178	1.5722064093195364E-5
86-87	1.5722064093195364E-5	1.257765127455629E-4	1.5722064093195364E-5	17.249737755970926	1.5722064093195364E-5
88	1.5722064093195364E-5	1.4149857683875828E-4	1.5722064093195364E-5	17.7956471264469	1.5722064093195364E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6305	0.0	46.830925	1
GTATCAA	10530	0.0	34.157906	1
TCAACGC	10990	0.0	31.740705	4
ATCAACG	11315	0.0	30.912115	3
CAACGCA	11585	0.0	30.0303	5
AACGCAG	12235	0.0	28.659369	6
TATCAAC	12985	0.0	27.081327	2
GTACATG	18115	0.0	25.43583	1
TACATGG	18150	0.0	23.907568	2
ACGCAGA	14425	0.0	23.721409	7
CGCAGAG	14940	0.0	22.80932	8
ACATGGG	18525	0.0	22.078592	3
GTGGTAT	3550	0.0	19.866486	1
GCAGAGT	16970	0.0	19.30527	9
CATGGGG	12815	0.0	19.00297	4
GAGTACA	13740	0.0	17.828268	1
CAGAGTA	16140	0.0	17.691624	10-11
AGAGTAC	14535	0.0	16.524595	10-11
TGGTATC	3895	0.0	16.052938	2
AGTACAT	14175	0.0	16.05213	2
>>END_MODULE