FastQCFastQC Report
Wed 25 May 2016
SRR2049354_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049354_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6360488
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT199430.31354512421059516No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128460.20196563534118764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127870.20103803355968913No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT96700.1520323597811992TruSeq Adapter, Index 11 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69380.10907968067858943No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100050.055.1708641
GTATCAA177250.039.656441
ATCAACG218850.031.6527633
TCAACGC219050.031.6238634
GTGGTAT43550.031.3089561
CAACGCA225700.030.6921025
TATCAAC228000.030.4237122
AACGCAG236150.029.339636
TGGTATC49950.026.0618342
ACGCAGA273300.025.196567
CGCAGAG275950.024.9036528
GTACATG178000.024.2747211
TACATGG176650.022.9858632
GCAGAGT297300.022.4031039
ACATGGG179250.021.7610383
GAGTACT203100.020.2557512-13
CAGAGTA290550.020.0743110-11
AGAGTAC274200.019.81318910-11
GAGTACA136900.018.8207041
GTACTTT218900.018.7533814-15
Produced by FastQC (version 0.11.5)