##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049354_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6360488
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.776048473010245	31.0	31.0	34.0	30.0	34.0
2	31.990146667991514	33.0	31.0	34.0	30.0	34.0
3	31.95159475184923	33.0	31.0	34.0	30.0	34.0
4	35.665958335272386	37.0	35.0	37.0	33.0	37.0
5	35.76435424451709	37.0	35.0	37.0	35.0	37.0
6	35.804874248642555	37.0	35.0	37.0	35.0	37.0
7	35.749889945551345	37.0	35.0	37.0	33.0	37.0
8	35.74797358315903	37.0	35.0	37.0	33.0	37.0
9	37.49585047562388	39.0	37.0	39.0	35.0	39.0
10-11	37.45673759623475	39.0	37.0	39.0	34.5	39.0
12-13	37.40056116763368	39.0	37.0	39.0	34.0	39.0
14-15	38.679017238928836	40.0	38.0	41.0	34.0	41.0
16-17	38.700847167701596	40.0	38.0	41.0	34.0	41.0
18-19	38.76703139759088	40.0	38.0	41.0	34.5	41.0
20-21	38.80027129993799	40.0	38.0	41.0	34.5	41.0
22-23	38.762788641374684	40.0	38.0	41.0	34.0	41.0
24-25	38.737714307455654	40.0	38.0	41.0	34.0	41.0
26-27	38.6164456249269	40.0	38.0	41.0	34.0	41.0
28-29	38.52934932036661	40.0	38.0	41.0	34.0	41.0
30-31	38.428576785303264	40.0	38.0	41.0	34.0	41.0
32-33	38.29313104591975	40.0	38.0	41.0	34.0	41.0
34-35	38.190540883026586	40.0	38.0	41.0	34.0	41.0
36-37	38.07048099139563	40.0	38.0	41.0	33.0	41.0
38-39	37.96462881464441	40.0	37.5	41.0	33.0	41.0
40-41	37.86955615669741	40.0	37.0	41.0	33.0	41.0
42-43	37.74508913467017	40.0	37.0	41.0	33.0	41.0
44-45	37.57769168026101	40.0	37.0	41.0	33.0	41.0
46-47	37.361952966501946	40.0	37.0	41.0	32.0	41.0
48-49	37.164670305171555	40.0	37.0	41.0	31.5	41.0
50-51	37.009747836958425	40.0	36.5	41.0	31.0	41.0
52-53	36.87542418128923	40.0	36.0	41.0	31.0	41.0
54-55	36.71814340346212	39.5	36.0	41.0	31.0	41.0
56-57	36.56871713302502	39.0	35.5	41.0	31.0	41.0
58-59	36.36242478564537	39.0	35.0	41.0	30.5	41.0
60-61	36.15005098665385	39.0	35.0	41.0	30.0	41.0
62-63	35.92496188971664	39.0	35.0	41.0	30.0	41.0
64-65	35.6657002575903	38.0	35.0	40.0	29.5	41.0
66-67	35.34187361095564	37.5	35.0	40.0	29.0	41.0
68-69	34.989277159236835	37.0	34.5	40.0	29.0	41.0
70-71	34.61417787440209	36.5	34.0	39.0	28.0	41.0
72-73	34.30027523045402	36.0	34.0	39.0	28.0	41.0
74-75	33.98283527930562	35.5	34.0	39.0	28.0	40.5
76-77	33.64725316673815	35.0	34.0	37.5	27.5	40.0
78-79	33.29947261908205	35.0	34.0	37.0	27.0	39.0
80-81	32.967665688544656	35.0	33.0	37.0	26.0	39.0
82-83	32.664147467930135	35.0	33.0	36.0	26.0	38.5
84-85	32.39805247647665	35.0	33.0	36.0	26.0	37.0
86-87	32.167935699273386	35.0	33.0	35.5	26.0	37.0
88-89	31.937897611000917	35.0	33.0	35.0	25.5	36.5
90-91	31.736529728536553	35.0	33.0	35.0	25.0	36.0
92-93	31.549147172355326	35.0	33.0	35.0	25.0	36.0
94-95	31.389775202783184	35.0	33.0	35.0	24.5	36.0
96-97	31.18460855519262	35.0	33.0	35.0	24.0	35.5
98-99	31.021047205811882	35.0	33.0	35.0	23.5	35.0
100	30.895085880202902	35.0	33.0	35.0	21.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2156295160874464
1101	2	0.23053918578428778
1101	3	0.38745119446306475
1101	4	0.2167172864056326
1101	5	0.17491502025971783
1101	6	0.16867296630802997
1101	7	0.06933772908946167
1101	8	0.1345984953582473
1101	9	0.24275009148197313
1101	10-11	0.2363517256526606
1101	12-13	0.18990141285760131
1101	14-15	0.3318544553865692
1101	16-17	0.23990357332186107
1101	18-19	0.32195863204767505
1101	20-21	0.30626049650612686
1101	22-23	0.2692389535121009
1101	24-25	0.29212418027427844
1101	26-27	0.35193678705616094
1101	28-29	0.3315640080251825
1101	30-31	0.3281566190156653
1101	32-33	0.3426690127703296
1101	34-35	0.33542853021343433
1101	36-37	0.34503058453139346
1101	38-39	0.37814242793532316
1101	40-41	0.4021939811399804
1101	42-43	0.37054653548807437
1101	44-45	0.3576386266530278
1101	46-47	0.37537946633461416
1101	48-49	0.465813185202542
1101	50-51	0.36376635775921073
1101	52-53	0.35824307599468597
1101	54-55	0.3631314106867265
1101	56-57	0.34316526134101366
1101	58-59	0.3829524829084576
1101	60-61	0.4147051948357472
1101	62-63	0.3799630360786992
1101	64-65	0.25692764198323914
1101	66-67	0.28277292006892907
1101	68-69	0.326691633072258
1101	70-71	0.33445333457930104
1101	72-73	0.227142221078374
1101	74-75	0.30802019650715806
1101	76-77	0.2727167636499388
1101	78-79	0.29284427521947975
1101	80-81	0.3539504704079377
1101	82-83	0.30908183816774226
1101	84-85	0.35934647510063655
1101	86-87	0.22711171612084513
1101	88-89	0.3194052288788676
1101	90-91	0.32598485915998765
1101	92-93	0.3215664767964128
1101	94-95	0.3384819484126034
1101	96-97	0.4057475060368212
1101	98-99	0.45790970982931256
1101	100	0.45761524078606186
1102	1	0.25132402769489204
1102	2	0.21288035368171165
1102	3	0.4020891607002248
1102	4	0.22794715009831634
1102	5	0.1995893426689932
1102	6	0.17266370788830443
1102	7	0.13795890205769723
1102	8	0.1466685262196421
1102	9	0.1625287138344902
1102	10-11	0.21868140238034783
1102	12-13	0.200609136686694
1102	14-15	0.2688736102745253
1102	16-17	0.24288419385761983
1102	18-19	0.23472690626077508
1102	20-21	0.22958674204398477
1102	22-23	0.2414835284206731
1102	24-25	0.2432691310438031
1102	26-27	0.31818276147377844
1102	28-29	0.2641769027337446
1102	30-31	0.2713416522445584
1102	32-33	0.2534029301699121
1102	34-35	0.306967001600313
1102	36-37	0.357393194216975
1102	38-39	0.30322892128786805
1102	40-41	0.33525072883880824
1102	42-43	0.3419492116818077
1102	44-45	0.35711575219410463
1102	46-47	0.33583677711124693
1102	48-49	0.35880321485500843
1102	50-51	0.40533952093507253
1102	52-53	0.4757193506644981
1102	54-55	0.37221016938013207
1102	56-57	0.4026053045100326
1102	58-59	0.39524924137015915
1102	60-61	0.3938210694827262
1102	62-63	0.44367296705192416
1102	64-65	0.400913573013554
1102	66-67	0.3790461500719857
1102	68-69	0.41915561901167564
1102	70-71	0.4257379277226292
1102	72-73	0.4951851415943267
1102	74-75	0.46129791887545935
1102	76-77	0.45963281283676594
1102	78-79	0.4870087339024778
1102	80-81	0.46746638426130716
1102	82-83	0.5056686774097443
1102	84-85	0.48944388453972465
1102	86-87	0.4648176336849801
1102	88-89	0.4704305245639837
1102	90-91	0.4695854949417395
1102	92-93	0.5389561111946186
1102	94-95	0.4815543052126898
1102	96-97	0.5581567793163593
1102	98-99	0.551381613027278
1102	100	0.5873242067109139
1103	1	0.15249631972084643
1103	2	0.19414010567317774
1103	3	0.33363223370671236
1103	4	0.17070398675759435
1103	5	0.20002253639486867
1103	6	0.13081842579912006
1103	7	0.14956274536885417
1103	8	0.16202124035284982
1103	9	0.2210878827595124
1103	10-11	0.19628877693894253
1103	12-13	0.21180031678031241
1103	14-15	0.3062391354449261
1103	16-17	0.26933159469525947
1103	18-19	0.2773472150396188
1103	20-21	0.2802406387344689
1103	22-23	0.24252750476953366
1103	24-25	0.2575316751843886
1103	26-27	0.28790642083158247
1103	28-29	0.3336018431952752
1103	30-31	0.2887392050079711
1103	32-33	0.30828097895039974
1103	34-35	0.35283215722156314
1103	36-37	0.34391080939311536
1103	38-39	0.3840482938755301
1103	40-41	0.45004911924695534
1103	42-43	0.46220563773091783
1103	44-45	0.45751712363205854
1103	46-47	0.37272394143744236
1103	48-49	0.3313806148714349
1103	50-51	0.33002540415755277
1103	52-53	0.3720977689941378
1103	54-55	0.358437277863338
1103	56-57	0.336302027840901
1103	58-59	0.4030426223342687
1103	60-61	0.3716953406496799
1103	62-63	0.32220010838394586
1103	64-65	0.35879420863809486
1103	66-67	0.3538038900736282
1103	68-69	0.33880126243781206
1103	70-71	0.40335556753539237
1103	72-73	0.405050050849475
1103	74-75	0.38022807408609793
1103	76-77	0.38249045429196826
1103	78-79	0.3489831528890974
1103	80-81	0.37717320860227943
1103	82-83	0.3942218924873515
1103	84-85	0.3665852165591019
1103	86-87	0.3432554673057382
1103	88-89	0.3779631680722382
1103	90-91	0.40688018985839136
1103	92-93	0.4213132188566
1103	94-95	0.3735268764535533
1103	96-97	0.45638294339618213
1103	98-99	0.49425650010948274
1103	100	0.5512644218706413
1104	1	0.14708681186808548
1104	2	0.18873453668917506
1104	3	0.28818719917916624
1104	4	0.16710803975747268
1104	5	0.16434949900827434
1104	6	0.18247375941169963
1104	7	0.20164160344276638
1104	8	0.16120507792543037
1104	9	0.20119630732729377
1104	10-11	0.2032809414722152
1104	12-13	0.1837676039949372
1104	14-15	0.23279280301209582
1104	16-17	0.24633777583711236
1104	18-19	0.29802055985010867
1104	20-21	0.31264335828317513
1104	22-23	0.2988150441101851
1104	24-25	0.2898354906993106
1104	26-27	0.3262889676080505
1104	28-29	0.3603231768770243
1104	30-31	0.35231012097748504
1104	32-33	0.3833142671948906
1104	34-35	0.3422952029470281
1104	36-37	0.36809445482576564
1104	38-39	0.3808159717106534
1104	40-41	0.34009141885460537
1104	42-43	0.36321014533389473
1104	44-45	0.33895996007869655
1104	46-47	0.3320175821818694
1104	48-49	0.2474578042099722
1104	50-51	0.21892447112600166
1104	52-53	0.22001367510686975
1104	54-55	0.22213101928910106
1104	56-57	0.3197658642222123
1104	58-59	0.3703724920249627
1104	60-61	0.3641985926181519
1104	62-63	0.3236836208557534
1104	64-65	0.26838629074333653
1104	66-67	0.3463961748865074
1104	68-69	0.2695015740887925
1104	70-71	0.30646679350348904
1104	72-73	0.3191293210635635
1104	74-75	0.3080755639699291
1104	76-77	0.2665412781880221
1104	78-79	0.3168948943686516
1104	80-81	0.2637283844679743
1104	82-83	0.31066957566395814
1104	84-85	0.35776930775639926
1104	86-87	0.3669868393386224
1104	88-89	0.3193805651909081
1104	90-91	0.2513451906077364
1104	92-93	0.3632825379510827
1104	94-95	0.3924572718391204
1104	96-97	0.4422595290310305
1104	98-99	0.45515945695101223
1104	100	0.42145001944506433
1105	1	0.18645143849281354
1105	2	0.1721791847652625
1105	3	0.2769995764710451
1105	4	0.16114869639949347
1105	5	0.14963161690126014
1105	6	0.10560689446302973
1105	7	0.10222747331483362
1105	8	0.15537087514789505
1105	9	0.14366267993605675
1105	10-11	0.16103849261791936
1105	12-13	0.19318474101416427
1105	14-15	0.2322263985643147
1105	16-17	0.19093113861278255
1105	18-19	0.1741921282939458
1105	20-21	0.1853599366806975
1105	22-23	0.17176888484567598
1105	24-25	0.20977573658604598
1105	26-27	0.23114980781090821
1105	28-29	0.23338724364668195
1105	30-31	0.21992439997679725
1105	32-33	0.23574957506492922
1105	34-35	0.20359558693378688
1105	36-37	0.20414294742878525
1105	38-39	0.19895367020202315
1105	40-41	0.19732425433596745
1105	42-43	0.1946668876680917
1105	44-45	0.18674739970105492
1105	46-47	0.24186637312149628
1105	48-49	0.21016138319859579
1105	50-51	0.21315792054764415
1105	52-53	0.22760658382254206
1105	54-55	0.1898682804850509
1105	56-57	0.1851956304132898
1105	58-59	0.17971982417122234
1105	60-61	0.2044015800497334
1105	62-63	0.23493871304271607
1105	64-65	0.1673820769843104
1105	66-67	0.25764812656794334
1105	68-69	0.282005902276417
1105	70-71	0.279277141686201
1105	72-73	0.31841331435224873
1105	74-75	0.3073142540388005
1105	76-77	0.2615965408073819
1105	78-79	0.25008318012313424
1105	80-81	0.2928106019892027
1105	82-83	0.2566411692775148
1105	84-85	0.25023982583246607
1105	86-87	0.23004117282513192
1105	88-89	0.2757868995563122
1105	90-91	0.34476866939253625
1105	92-93	0.346214130203375
1105	94-95	0.3494476031932834
1105	96-97	0.42743175778042897
1105	98-99	0.4010579966958794
1105	100	0.410043600571516
1106	1	0.13850265841784548
1106	2	0.12954424039847368
1106	3	0.24263057432077417
1106	4	0.16147868914832486
1106	5	0.12228301141317388
1106	6	0.10411834165017098
1106	7	0.149197768358313
1106	8	0.13983149856847632
1106	9	0.15781573282541217
1106	10-11	0.15884090742487444
1106	12-13	0.16824196787626278
1106	14-15	0.16916521759632275
1106	16-17	0.14236829640780968
1106	18-19	0.18028628731011054
1106	20-21	0.18475169814622205
1106	22-23	0.18270508034515132
1106	24-25	0.13634054880505886
1106	26-27	0.24607201524286637
1106	28-29	0.2688460995317712
1106	30-31	0.25856000798052037
1106	32-33	0.20289800174278128
1106	34-35	0.19648048398042306
1106	36-37	0.22961977012345614
1106	38-39	0.23078730212065324
1106	40-41	0.27089078921743237
1106	42-43	0.26560341201650317
1106	44-45	0.25744096628322666
1106	46-47	0.30105868496354304
1106	48-49	0.300997336966617
1106	50-51	0.3035038069280347
1106	52-53	0.2567601299852882
1106	54-55	0.27495580104223194
1106	56-57	0.31758831100606244
1106	58-59	0.2995585595172372
1106	60-61	0.2329410805799057
1106	62-63	0.24749053561915701
1106	64-65	0.2362621462207528
1106	66-67	0.20333946324643648
1106	68-69	0.26705035352269135
1106	70-71	0.2988659848429691
1106	72-73	0.326924315008732
1106	74-75	0.32943545939440355
1106	76-77	0.2730016649775422
1106	78-79	0.3231892660169322
1106	80-81	0.2902290994223691
1106	82-83	0.3460084386469191
1106	84-85	0.3117933059614728
1106	86-87	0.2824586798621169
1106	88-89	0.2716837778121466
1106	90-91	0.2676997724629189
1106	92-93	0.3218824386583883
1106	94-95	0.2339489257928733
1106	96-97	0.2946207791535187
1106	98-99	0.3385197295141573
1106	100	0.3837083776949832
1107	1	0.09692543724201741
1107	2	0.11177594478816388
1107	3	0.2086577501956448
1107	4	0.15357439024168684
1107	5	0.1267900428633979
1107	6	0.1310479322835505
1107	7	0.08233528360953102
1107	8	0.08617351957987296
1107	9	0.12452474662124047
1107	10-11	0.16005448277781653
1107	12-13	0.1295224407966984
1107	14-15	0.17231932023085506
1107	16-17	0.15738127776855038
1107	18-19	0.09153605749360594
1107	20-21	0.12956858153211925
1107	22-23	0.1363262032299133
1107	24-25	0.16610652109205404
1107	26-27	0.218724038391926
1107	28-29	0.18877388507069526
1107	30-31	0.1523974804679682
1107	32-33	0.19239079124668024
1107	34-35	0.16115921786963128
1107	36-37	0.18142626404075912
1107	38-39	0.15521341252294718
1107	40-41	0.14492856448045188
1107	42-43	0.15972679172335802
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2216	68-69	-0.33523327375905154
2216	70-71	-0.48799077880480013
2216	72-73	-0.47397058140639103
2216	74-75	-0.4171846105783388
2216	76-77	-0.4126944288835759
2216	78-79	-0.6262820475475195
2216	80-81	-0.7653610635723567
2216	82-83	-0.717221237412609
2216	84-85	-0.7658427469699731
2216	86-87	-0.6152005590091925
2216	88-89	-0.90572050441234
2216	90-91	-0.7063684647757498
2216	92-93	-0.7797564963292984
2216	94-95	-0.765017714615265
2216	96-97	-0.8817432795123423
2216	98-99	-0.9860789361808386
2216	100	-0.9863285622074969
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	369.0
3	33.0
4	56.0
5	111.0
6	324.0
7	826.0
8	2062.0
9	3566.0
10	5230.0
11	7439.0
12	10385.0
13	14122.0
14	17051.0
15	21293.0
16	26381.0
17	35985.0
18	36307.0
19	22958.0
20	19267.0
21	19859.0
22	22416.0
23	26105.0
24	30038.0
25	36417.0
26	43703.0
27	48605.0
28	60870.0
29	79959.0
30	104361.0
31	133609.0
32	172142.0
33	224548.0
34	304684.0
35	436258.0
36	708396.0
37	1273134.0
38	1736255.0
39	672494.0
40	2870.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.27829568963225	20.72258730590888	14.32963038284793	23.66948662161093
2	15.659663220809472	20.714699878374113	39.503509793588165	24.12212710722825
3	17.768322179052927	25.32734909648442	31.296686669324743	25.60764205513791
4	12.52366367543869	17.40110466384099	37.919124746265496	32.15610691445482
5	12.590716309817736	37.837489827824534	35.82668499649713	13.745108865860606
6	30.58090825735384	38.19999345962134	17.140618770132104	14.078479512892722
7	26.354361489244223	33.06452272215591	22.245274261974867	18.335841526625003
8	24.965120151135007	36.21775087942499	20.375444586399045	18.441684383040958
9	24.61237250978227	16.3217822280303	21.490961070911542	37.574884191275885
10-11	23.782821381000954	26.92761939021031	28.709597439693308	20.579961789095428
12-13	24.34808315543702	24.393909937593154	28.989902850682235	22.268104056287587
14-15	21.693932170831786	26.45509088230619	26.41696482293236	25.434012123929662
16-17	20.93383400770507	29.914308461866447	27.209036476446464	21.942821053982023
18-19	20.743048682608322	28.213445987216385	29.971071388423997	21.0724339417513
20-21	21.72883110675959	27.388126608319418	29.77149139235899	21.111550892562004
22-23	21.396015525852732	27.465565535223085	29.84192407878138	21.296494860142808
24-25	21.41038660114334	27.623546828126106	29.754603541900828	21.21146302882973
26-27	21.132921734644857	27.604041422322688	29.89240519215245	21.37063165088
28-29	20.978627596379106	27.3682669759311	30.166347023021967	21.48675840466783
30-31	21.43645435982698	27.582107503552205	29.92827200745226	21.053166129168556
32-33	21.02101320718764	27.64642929239887	30.016102568424124	21.316454931989366
34-35	21.1000170898971	27.36552397042803	30.270185456041627	21.26427348363324
36-37	21.535212027136932	27.441665986686857	29.639690885110454	21.383431101065753
38-39	21.253816247369787	27.279786500037456	29.95032835233311	21.516068900259643
40-41	21.088887945563854	26.972846388951776	30.228143159006866	21.710122506477497
42-43	21.10042687170847	26.97966451792237	30.221450999055254	21.698457611313902
44-45	20.959316793110325	27.221561850456283	30.027020782804804	21.792100573628588
46-47	21.186315567544163	27.245053559611748	29.5757885053698	21.992842367474292
48-49	21.038274326526388	27.240669019911127	29.41945747922883	22.301599174333656
50-51	20.883440403630992	27.266906512937815	29.349357114411323	22.500295969019867
52-53	20.762144801522155	27.341254356211454	29.54181129723677	22.354789545029618
54-55	20.761946043712065	27.589797504537213	29.162803937165897	22.485452514584825
56-57	20.90318281910968	27.464632286145253	28.84810097128165	22.78408392346342
58-59	20.591077985381766	27.35151848983736	29.17601901302897	22.881384511751904
60-61	20.87343121067316	27.77072729439517	28.514625787558735	22.841215707372935
62-63	20.50495115351351	27.91107749357473	28.450513698980117	23.13345765393164
64-65	20.522542025036064	27.71228422075149	28.446436577077034	23.31873717713541
66-67	20.645917126569753	27.75940279208943	28.165218591666747	23.429461489674075
68-69	20.95563981316863	27.80345318556038	27.945221266375576	23.295685734895418
70-71	20.913707140007507	27.52552978655247	27.93877803697451	23.62198503646551
72-73	20.732787164637347	27.518604093938333	27.991952742802845	23.756655998621476
74-75	20.821328440580448	27.47042553350384	27.92249420341636	23.78575182249935
76-77	20.894087682475295	27.473024707371334	27.78548687592859	23.84740073422478
78-79	20.929858507004294	27.475577134621204	27.81964220283087	23.77492215554364
80-81	20.877667153461175	27.277420436140314	27.84188928992595	24.003023120472562
82-83	20.720913991106237	27.189543696225964	28.03591182457992	24.05363048808788
84-85	20.77926855929439	27.036070015764167	27.96554507834169	24.219116346599744
86-87	20.896330560394087	27.12432034531167	27.90224323977159	24.07710585452265
88-89	20.80301240691863	26.921468705596574	27.964750436787213	24.31076845069758
90-91	20.78687069871459	26.646508313748328	27.98113707700948	24.585483910527604
92-93	20.732308317819847	26.635959647669033	28.01342824145899	24.618303793052135
94-95	20.57340720156277	26.35096811136742	28.36693620819137	24.708688478878447
96-97	20.66961402303925	26.382935268846825	28.308834396494742	24.63861631161918
98-99	20.698962114527056	26.390434394671626	28.20723905955509	24.70336443124623
100	20.69056110212058	26.38983157172835	28.219451219445084	24.700156106705982
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	560.0
1	417.5
2	472.0
3	674.5
4	459.5
5	349.5
6	848.0
7	1990.0
8	3320.5
9	3989.0
10	3288.0
11	2322.5
12	2318.0
13	2730.5
14	3163.5
15	3596.0
16	4192.5
17	4852.5
18	5648.0
19	6588.0
20	7990.0
21	10050.5
22	12728.0
23	16891.0
24	22907.5
25	30496.5
26	40379.5
27	52774.5
28	67489.0
29	83771.5
30	99432.0
31	113713.5
32	126859.5
33	139746.5
34	153295.0
35	167033.5
36	180595.0
37	193186.5
38	205855.0
39	216024.5
40	223527.5
41	232084.5
42	238563.0
43	241988.0
44	242709.0
45	241761.0
46	239680.5
47	233139.5
48	224528.5
49	218295.5
50	211819.0
51	201533.0
52	188423.0
53	176670.5
54	166306.0
55	155310.5
56	141590.0
57	126402.5
58	112872.0
59	100105.0
60	86738.0
61	73082.5
62	61516.0
63	51303.0
64	41420.5
65	33236.5
66	26559.0
67	20542.5
68	15613.0
69	11735.0
70	8676.5
71	6411.0
72	4572.0
73	3103.5
74	2184.0
75	1468.5
76	878.0
77	553.0
78	362.0
79	217.0
80	123.0
81	72.5
82	42.5
83	19.5
84	11.0
85	2.0
86	3.5
87	4.5
88	2.5
89	1.5
90	0.5
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04064153568091002
2	0.0
3	0.0
4	1.5722064093195367E-5
5	0.0
6	0.0
7	0.0
8	0.00128920925564202
9	0.0
10-11	0.0
12-13	0.04483932679379318
14-15	1.4149857683875828E-4
16-17	0.0
18-19	4.7166192279586095E-5
20-21	8.647135251257452E-5
22-23	0.0
24-25	7.861032046597682E-5
26-27	7.074928841937914E-5
28-29	3.065802498173096E-4
30-31	7.861032046597684E-6
32-33	1.8866476911834438E-4
34-35	7.861032046597682E-5
36-37	7.861032046597682E-5
38-39	2.908581857241142E-4
40-41	1.2577651274556294E-4
42-43	4.559398587026656E-4
44-45	2.1224786525813743E-4
46-47	7.861032046597682E-5
48-49	7.782421726131706E-4
50-51	3.930516023298841E-4
52-53	4.7166192279586095E-4
54-55	3.8519057028328646E-4
56-57	4.087736664230795E-4
58-59	1.1791548069896524E-4
60-61	1.5722064093195363E-4
62-63	8.018252687529636E-4
64-65	3.694685061900911E-4
66-67	0.0014149857683875828
68-69	0.0015722064093195364
70-71	4.009126343764818E-4
72-73	9.433238455917219E-4
74-75	9.511848776383195E-4
76-77	5.974384355414239E-4
78-79	0.0032780503634312337
80-81	0.0027120560560762006
82-83	0.0011948768710828479
84-85	0.0027906663765421775
86-87	0.0013992637042943873
88-89	0.0019731190436960183
90-91	0.0028299715367751657
92-93	0.0025391133510510515
94-95	0.003828322606693071
96-97	0.0033330775877574172
98-99	0.003152273850685671
100	0.0014935960888535595
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6360488.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.70172062303877
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.94098040630026	45.33807196056599
2	13.994091667911604	16.709427022617067
3	4.552117201881814	8.15307688290231
4	1.9597475593579037	4.680012051218705
5	1.0347697796569864	3.088876814712239
6	0.6132719835455791	2.1968035576544995
7	0.38908797233540116	1.6260454995506928
8	0.27683049147785727	1.3221805329719638
9	0.19732921303583983	1.0602804192686828
>10	0.9710601198888225	10.132496057139857
>50	0.049622512152747766	2.0284635123381465
>100	0.019958969567396234	1.9830994878811976
>500	6.582256596172214E-4	0.2700158967307207
>1k	3.159314861254517E-4	0.35488200633935
>5k	7.898287109063579E-5	0.33990270898647573
>10k+	7.898287109063579E-5	0.716365589122202
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	19943	0.31354512421059516	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12846	0.20196563534118764	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12787	0.20103803355968913	No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	9670	0.1520323597811992	TruSeq Adapter, Index 11 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6938	0.10907968067858943	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.5722064093195364E-5	0.0
3	0.0	0.0	0.0	3.144412818639073E-5	0.0
4	0.0	0.0	0.0	3.144412818639073E-5	0.0
5	0.0	1.5722064093195364E-5	0.0	6.288825637278146E-5	0.0
6	0.0	1.5722064093195364E-5	0.0	7.861032046597683E-5	0.0
7	0.0	1.5722064093195364E-5	0.0	7.861032046597683E-5	0.0
8	0.0	1.5722064093195364E-5	0.0	7.861032046597683E-5	0.0
9	0.0	1.5722064093195364E-5	0.0	1.4149857683875828E-4	0.0
10-11	0.0	1.5722064093195364E-5	0.0	1.8080373707174668E-4	0.0
12-13	0.0	1.5722064093195364E-5	0.0	2.4369199344452815E-4	0.0
14-15	0.0	1.5722064093195364E-5	0.0	2.515530254911258E-4	0.0
16-17	0.0	1.5722064093195364E-5	0.0	2.8299715367751657E-4	0.0
18-19	0.0	1.5722064093195364E-5	0.0	3.8519057028328646E-4	0.0
20-21	0.0	1.5722064093195364E-5	0.0	6.052994675880216E-4	0.0
22-23	0.0	1.5722064093195364E-5	0.0	0.0020438683321153976	0.0
24-25	0.0	1.5722064093195364E-5	0.0	0.004771646452284793	0.0
26-27	0.0	1.5722064093195364E-5	0.0	0.010360840237415746	0.0
28-29	0.0	1.5722064093195364E-5	0.0	0.03351944064669252	0.0
30-31	0.0	1.5722064093195364E-5	0.0	0.10249999685558718	0.0
32-33	0.0	1.5722064093195364E-5	0.0	0.2561988954306651	0.0
34-35	0.0	1.5722064093195364E-5	0.0	0.5206440134782111	0.0
36-37	0.0	1.5722064093195364E-5	0.0	0.9305182243878143	0.0
38-39	0.0	1.5722064093195364E-5	0.0	1.4505726604625306	0.0
40-41	0.0	1.5722064093195364E-5	0.0	1.9545984521942343	0.0
42-43	0.0	1.5722064093195364E-5	0.0	2.443004373249348	0.0
44-45	0.0	1.5722064093195364E-5	0.0	3.0135344960952684	0.0
46-47	0.0	3.144412818639073E-5	0.0	3.669584786576124	0.0
48-49	7.861032046597682E-6	3.144412818639073E-5	0.0	4.314047915820296	0.0
50-51	1.5722064093195364E-5	3.144412818639073E-5	0.0	4.931704925785569	0.0
52-53	1.5722064093195364E-5	3.144412818639073E-5	0.0	5.504640524437748	0.0
54-55	1.5722064093195364E-5	3.144412818639073E-5	0.0	6.111472893274856	0.0
56-57	1.5722064093195364E-5	3.144412818639073E-5	0.0	6.790312315658799	0.0
58-59	1.5722064093195364E-5	3.144412818639073E-5	0.0	7.49017999876739	0.0
60-61	1.5722064093195364E-5	3.144412818639073E-5	0.0	8.171700033079222	0.0
62-63	1.5722064093195364E-5	3.144412818639073E-5	0.0	8.803404707311765	0.0
64-65	1.5722064093195364E-5	3.144412818639073E-5	0.0	9.447545534242028	0.0
66-67	1.5722064093195364E-5	3.144412818639073E-5	0.0	10.141132252745386	0.0
68-69	1.5722064093195364E-5	3.144412818639073E-5	0.0	10.871437851938405	0.0
70-71	1.5722064093195364E-5	3.144412818639073E-5	0.0	11.60234875059901	0.0
72-73	1.5722064093195364E-5	3.144412818639073E-5	0.0	12.303379866450499	0.0
74-75	1.5722064093195364E-5	3.144412818639073E-5	0.0	12.984451821935675	0.0
76-77	1.5722064093195364E-5	3.144412818639073E-5	0.0	13.683659178352354	0.0
78-79	1.5722064093195364E-5	3.144412818639073E-5	0.0	14.430897440573741	0.0
80-81	1.5722064093195364E-5	3.144412818639073E-5	0.0	15.193118829875946	0.0
82-83	1.5722064093195364E-5	3.144412818639073E-5	0.0	15.926057874804576	0.0
84-85	1.5722064093195364E-5	3.144412818639073E-5	1.5722064093195364E-5	16.62548534011856	0.0
86-87	1.5722064093195364E-5	3.144412818639073E-5	1.5722064093195364E-5	17.33501423161242	0.0
88	1.5722064093195364E-5	3.144412818639073E-5	1.5722064093195364E-5	17.88523144764993	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10005	0.0	55.170864	1
GTATCAA	17725	0.0	39.65644	1
ATCAACG	21885	0.0	31.652763	3
TCAACGC	21905	0.0	31.623863	4
GTGGTAT	4355	0.0	31.308956	1
CAACGCA	22570	0.0	30.692102	5
TATCAAC	22800	0.0	30.423712	2
AACGCAG	23615	0.0	29.33963	6
TGGTATC	4995	0.0	26.061834	2
ACGCAGA	27330	0.0	25.19656	7
CGCAGAG	27595	0.0	24.903652	8
GTACATG	17800	0.0	24.274721	1
TACATGG	17665	0.0	22.985863	2
GCAGAGT	29730	0.0	22.403103	9
ACATGGG	17925	0.0	21.761038	3
GAGTACT	20310	0.0	20.25575	12-13
CAGAGTA	29055	0.0	20.07431	10-11
AGAGTAC	27420	0.0	19.813189	10-11
GAGTACA	13690	0.0	18.820704	1
GTACTTT	21890	0.0	18.75338	14-15
>>END_MODULE