FastQCFastQC Report
Wed 25 May 2016
SRR2049353_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049353_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4247277
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42950.043.8898121
GTATCAA72200.036.591621
TCAACGC77950.032.5030754
ATCAACG81350.031.2024043
CAACGCA84150.030.1645325
TATCAAC90950.028.2706932
AACGCAG89650.028.2601836
GTACATG129700.025.8423561
TACATGG131600.024.5744822
ACGCAGA104650.023.9846527
CGCAGAG104400.023.636888
ACATGGG133550.023.406133
CATGGGG91150.020.3700584
GCAGAGT121000.019.2287569
GAGTACA97350.018.2531741
AGAGTAC104000.017.98792610-11
AGTACAT100000.017.063122
CAGAGTA115050.016.75053210-11
GTGGTAT26050.016.6020851
CATGGGT32350.015.256924
Produced by FastQC (version 0.11.5)