##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049353_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4247277
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.967020281465043	31.0	30.0	33.0	26.0	34.0
2	30.230174533000792	31.0	31.0	34.0	26.0	34.0
3	30.404513291692535	31.0	31.0	34.0	26.0	34.0
4	34.01919370928715	37.0	35.0	37.0	30.0	37.0
5	34.03001217015043	37.0	35.0	37.0	30.0	37.0
6	34.00755378092835	37.0	35.0	37.0	30.0	37.0
7	33.90817457867711	37.0	35.0	37.0	30.0	37.0
8	33.85291281920157	37.0	35.0	37.0	29.0	37.0
9	35.375536137624174	39.0	35.0	39.0	29.0	39.0
10-11	35.32103215778015	39.0	35.0	39.0	29.0	39.0
12-13	35.242634163017854	39.0	35.0	39.0	29.0	39.0
14-15	36.335655291613904	40.0	36.0	41.0	28.5	41.0
16-17	36.29361129495439	40.0	36.0	41.0	28.0	41.0
18-19	36.31616386216392	40.0	36.0	41.0	28.0	41.0
20-21	36.371129196423965	40.0	36.0	41.0	29.0	41.0
22-23	36.31953920123411	40.0	36.0	41.0	28.0	41.0
24-25	36.30933266184428	40.0	36.0	41.0	29.0	41.0
26-27	36.27568380870849	40.0	36.0	41.0	29.0	41.0
28-29	36.213850309268736	40.0	36.0	41.0	29.0	41.0
30-31	36.14867678750409	40.0	36.0	41.0	29.0	41.0
32-33	36.0724428851709	40.0	36.0	41.0	28.5	41.0
34-35	35.97427740173292	40.0	36.0	41.0	28.0	41.0
36-37	35.89400349918312	40.0	36.0	41.0	28.0	41.0
38-39	35.7806618216801	40.0	35.0	41.0	27.0	41.0
40-41	35.668366814785095	39.0	35.0	41.0	27.0	41.0
42-43	35.53085070740618	39.0	35.0	41.0	27.0	41.0
44-45	35.390699617660914	39.0	35.0	41.0	26.0	41.0
46-47	35.25504835215598	39.0	35.0	41.0	26.0	41.0
48-49	35.117396628475134	39.0	35.0	41.0	25.5	41.0
50-51	34.97829939040943	39.0	34.5	40.0	24.5	41.0
52-53	34.818122293412934	39.0	34.0	40.0	24.0	41.0
54-55	34.67164585686311	39.0	34.0	40.0	24.0	41.0
56-57	34.53021147431637	38.0	34.0	40.0	24.0	41.0
58-59	34.33011903862169	38.0	34.0	40.0	23.5	41.0
60-61	34.10608184961801	38.0	33.5	40.0	23.0	41.0
62-63	33.88284022916329	38.0	33.0	40.0	22.5	41.0
64-65	33.6399624983254	37.0	33.0	40.0	22.0	41.0
66-67	33.38011919166091	37.0	33.0	40.0	21.5	41.0
68-69	33.09440695768136	36.0	33.0	39.5	20.5	41.0
70-71	32.80810246188322	36.0	32.0	39.0	20.0	41.0
72-73	32.47003892140776	35.5	32.0	39.0	18.5	40.5
74-75	32.13039672241768	35.0	32.0	38.5	18.0	40.0
76-77	31.808875663160187	35.0	32.0	37.5	16.5	39.5
78-79	31.49411269855957	35.0	31.5	37.0	14.0	39.0
80-81	31.15238127864041	35.0	31.0	36.5	10.0	39.0
82-83	30.843032488815773	35.0	31.0	36.0	7.5	38.5
84-85	30.55363424612993	35.0	31.0	36.0	6.5	37.0
86-87	30.240454178053376	35.0	31.0	35.0	2.0	37.0
88-89	29.991028722638056	34.0	31.0	35.0	2.0	36.5
90-91	29.781293755975888	34.0	31.0	35.0	2.0	36.0
92-93	29.538735053070475	34.0	30.0	35.0	2.0	36.0
94-95	29.320460850563784	34.0	30.0	35.0	2.0	36.0
96-97	29.047320200683874	34.0	30.0	35.0	2.0	35.0
98-99	28.826385587754224	34.0	30.0	35.0	2.0	35.0
100	28.692790698605247	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3995087944309077
1101	2	0.33826519702788715
1101	3	0.4339856393048862
1101	4	0.47596755721704653
1101	5	0.5200897213176816
1101	6	0.5560275401530816
1101	7	0.48827544422136526
1101	8	0.5571123643385647
1101	9	0.6726251074210694
1101	10-11	0.7078761817520913
1101	12-13	0.7432472333632063
1101	14-15	0.855063989227645
1101	16-17	0.8198335929848071
1101	18-19	0.7647256405827889
1101	20-21	0.7508453706306568
1101	22-23	0.7211212683480497
1101	24-25	0.7577831841703144
1101	26-27	0.7244008360203225
1101	28-29	0.6911078817567997
1101	30-31	0.7299856121285231
1101	32-33	0.7730929255111505
1101	34-35	0.7975693291062811
1101	36-37	0.7752724159898392
1101	38-39	0.8279288817222508
1101	40-41	0.8770767832978166
1101	42-43	0.8628370081579888
1101	44-45	0.7689600313882607
1101	46-47	0.7916156269307351
1101	48-49	0.6401762871129648
1101	50-51	0.64030205837431
1101	52-53	0.6495704665983695
1101	54-55	0.6511330273698448
1101	56-57	0.6400766027317957
1101	58-59	0.7077072941735807
1101	60-61	0.7377107651799903
1101	62-63	0.7099088898251651
1101	64-65	0.7523630329038795
1101	66-67	0.6832139296369064
1101	68-69	0.6667300071165201
1101	70-71	0.7106653251018287
1101	72-73	0.8184455169456797
1101	74-75	0.725013523291949
1101	76-77	0.6585612041999909
1101	78-79	0.5658916967273626
1101	80-81	0.5816302949358203
1101	82-83	0.6384787691847613
1101	84-85	0.6120218664092043
1101	86-87	0.686953008634589
1101	88-89	0.5965609276271842
1101	90-91	0.6661693459762077
1101	92-93	0.6129046341987028
1101	94-95	0.6318723174425962
1101	96-97	0.6048921259085134
1101	98-99	0.6378449421645165
1101	100	0.6984803683232812
1102	1	0.17890951808003308
1102	2	0.2847453066011738
1102	3	0.43115624491412774
1102	4	0.46732415928408244
1102	5	0.3767888099892005
1102	6	0.5895777465830037
1102	7	0.6223117512265404
1102	8	0.5458674568576996
1102	9	0.6414625684638651
1102	10-11	0.6312765560687552
1102	12-13	0.713574803248612
1102	14-15	0.8001302575693074
1102	16-17	0.7970710977437818
1102	18-19	0.7085334437220396
1102	20-21	0.5712239447910434
1102	22-23	0.6215826824324253
1102	24-25	0.7261975137974872
1102	26-27	0.7288216158926133
1102	28-29	0.7106122067927814
1102	30-31	0.6357314983233024
1102	32-33	0.6971140100073043
1102	34-35	0.7205801654833479
1102	36-37	0.6837952098704179
1102	38-39	0.6273668484783599
1102	40-41	0.7566611173747546
1102	42-43	0.7400550118297033
1102	44-45	0.6623797045862716
1102	46-47	0.6071569142183435
1102	48-49	0.7294115752979735
1102	50-51	0.6929631690505502
1102	52-53	0.734959264563507
1102	54-55	0.7309578872616029
1102	56-57	0.6695020190744287
1102	58-59	0.7920048624053635
1102	60-61	0.7782212470764165
1102	62-63	0.6767826955851532
1102	64-65	0.670088776906951
1102	66-67	0.7246883879886283
1102	68-69	0.781948248721342
1102	70-71	0.7944032170913644
1102	72-73	0.7217549457337711
1102	74-75	0.7323510182631594
1102	76-77	0.7435609480311243
1102	78-79	0.809742087164679
1102	80-81	0.7340394917577377
1102	82-83	0.788932039208202
1102	84-85	0.8178343689135197
1102	86-87	0.8328774736789519
1102	88-89	0.8534862884562244
1102	90-91	0.8776459095025508
1102	92-93	0.7895955378718931
1102	94-95	0.7833293600332425
1102	96-97	0.7784371767074845
1102	98-99	0.8649967575085711
1102	100	0.8116772825367349
1103	1	0.1630548144427877
1103	2	0.24444587019885233
1103	3	0.31419225158614594
1103	4	0.28799587081705624
1103	5	0.31432849929233697
1103	6	0.43851305349765823
1103	7	0.4677364698585791
1103	8	0.3914622793426261
1103	9	0.5126467220427955
1103	10-11	0.48783540491618993
1103	12-13	0.496467002026435
1103	14-15	0.5991168398080333
1103	16-17	0.555671623710829
1103	18-19	0.5367109219336115
1103	20-21	0.5550696683045402
1103	22-23	0.511025842466978
1103	24-25	0.5805940377039533
1103	26-27	0.5400835155021468
1103	28-29	0.5305732930827887
1103	30-31	0.570600849180579
1103	32-33	0.5822451594554892
1103	34-35	0.5918439131110915
1103	36-37	0.5602443122889227
1103	38-39	0.6493557038752371
1103	40-41	0.6558676544758768
1103	42-43	0.7244932861062452
1103	44-45	0.5431720567591753
1103	46-47	0.5700098210648221
1103	48-49	0.6356086841548674
1103	50-51	0.6194949176204929
1103	52-53	0.6320045659053832
1103	54-55	0.6770246366262924
1103	56-57	0.6136678590800955
1103	58-59	0.6282538354626013
1103	60-61	0.5970710089405955
1103	62-63	0.6125925847806641
1103	64-65	0.6357667937248763
1103	66-67	0.6733183649506387
1103	68-69	0.6925030898588105
1103	70-71	0.5712780100271431
1103	72-73	0.5981106418429718
1103	74-75	0.6135712871929044
1103	76-77	0.6458132920459363
1103	78-79	0.5277281877696467
1103	80-81	0.5716917019109253
1103	82-83	0.49811449940878205
1103	84-85	0.6132762750724936
1103	86-87	0.6794850316904473
1103	88-89	0.5995848874094598
1103	90-91	0.63548644994734
1103	92-93	0.5343510800114935
1103	94-95	0.6191698899452831
1103	96-97	0.6581600481830847
1103	98-99	0.700983584873029
1103	100	0.6917960057380768
1104	1	-0.10462035994684271
1104	2	0.02902418682941388
1104	3	0.05034487013254818
1104	4	0.127417748577777
1104	5	0.18391751943119772
1104	6	0.2560121737572487
1104	7	0.23118261960183162
1104	8	0.2953746158852013
1104	9	0.33390866908353445
1104	10-11	0.2808425960101957
1104	12-13	0.22163251356504787
1104	14-15	0.3404900583336996
1104	16-17	0.33396709301084115
1104	18-19	0.3491977004680962
1104	20-21	0.3970792070328528
1104	22-23	0.34825873197711843
1104	24-25	0.3797776243115365
1104	26-27	0.31715333074190255
1104	28-29	0.3869089179216374
1104	30-31	0.3630646691646646
1104	32-33	0.31320771303757766
1104	34-35	0.37815178647420566
1104	36-37	0.3123979126588168
1104	38-39	0.3836866649833368
1104	40-41	0.37994019921629985
1104	42-43	0.391015073790399
1104	44-45	0.40001866184647383
1104	46-47	0.2892301917029627
1104	48-49	0.2566957528192475
1104	50-51	0.24842276489017223
1104	52-53	0.2656665738824344
1104	54-55	0.30360343176554494
1104	56-57	0.216659663477472
1104	58-59	0.24731669429801428
1104	60-61	0.25606119513202685
1104	62-63	0.19728598464352132
1104	64-65	0.30501531559500705
1104	66-67	0.31193603640871714
1104	68-69	0.3346912486266831
1104	70-71	0.37100331938408004
1104	72-73	0.3686528916066223
1104	74-75	0.46074397534761147
1104	76-77	0.4091632259455267
1104	78-79	0.3506251932218234
1104	80-81	0.4161003775714782
1104	82-83	0.3586063559081829
1104	84-85	0.3904307445779587
1104	86-87	0.43535376012804505
1104	88-89	0.33732534978774
1104	90-91	0.2789233398233897
1104	92-93	0.3111839347746539
1104	94-95	0.27682807400247356
1104	96-97	0.22665834006656382
1104	98-99	0.30773664837995796
1104	100	0.311260096283835
1105	1	0.10868488481317584
1105	2	0.1771468264938143
1105	3	0.18713593850445776
1105	4	0.22041303585405103
1105	5	0.17111073664847254
1105	6	0.23396105903803743
1105	7	0.2876930885798572
1105	8	0.3049010692096701
1105	9	0.34935766658687584
1105	10-11	0.277502228875008
1105	12-13	0.29576044291636094
1105	14-15	0.36320930662901674
1105	16-17	0.31118902115859726
1105	18-19	0.2549255376710491
1105	20-21	0.2431180982147083
1105	22-23	0.2893466445348096
1105	24-25	0.3332241200148047
1105	26-27	0.2873809586372431
1105	28-29	0.29890738416505513
1105	30-31	0.31484679570987595
1105	32-33	0.3292668893799018
1105	34-35	0.3457401068536967
1105	36-37	0.2740529399989029
1105	38-39	0.28192115406014295
1105	40-41	0.30036285376871064
1105	42-43	0.2691210907040045
1105	44-45	0.21800714837995372
1105	46-47	0.15463144532975548
1105	48-49	0.16807206610445036
1105	50-51	0.22439463954425065
1105	52-53	0.2278627311636754
1105	54-55	0.20934784337726597
1105	56-57	0.15897654394027683
1105	58-59	0.2016532290689952
1105	60-61	0.19310932946512338
1105	62-63	0.19658519235336058
1105	64-65	0.231244504603616
1105	66-67	0.27361348598325463
1105	68-69	0.18070228188089033
1105	70-71	0.28769706612474977
1105	72-73	0.20001331707347703
1105	74-75	0.24448850810400558
1105	76-77	0.31531345438164493
1105	78-79	0.2588041827571992
1105	80-81	0.30183817456834205
1105	82-83	0.2959676552295569
1105	84-85	0.28810563572319126
1105	86-87	0.269316278662032
1105	88-89	0.22981775301663987
1105	90-91	0.2424032340742066
1105	92-93	0.3007925267699427
1105	94-95	0.28433199276969034
1105	96-97	0.22360514015751676
1105	98-99	0.27572347858643553
1105	100	0.36796210661481865
1106	1	0.03130728637400537
1106	2	0.03528507641587453
1106	3	0.05775293455248587
1106	4	0.11888771855601021
1106	5	0.07224926955844779
1106	6	0.21165098022042628
1106	7	0.13415816914594814
1106	8	0.14732864866458328
1106	9	0.21114632285680557
1106	10-11	0.2508404923661516
1106	12-13	0.23530500570922186
1106	14-15	0.3014061741952432
1106	16-17	0.37101952217571466
1106	18-19	0.31192508856680234
1106	20-21	0.31384573210987554
1106	22-23	0.34837563426054174
1106	24-25	0.3343332962349663
1106	26-27	0.29762624885132993
1106	28-29	0.35760922307938614
1106	30-31	0.34667625823764325
1106	32-33	0.29483620905769214
1106	34-35	0.3615486548109672
1106	36-37	0.2608494676692672
1106	38-39	0.2929722068322178
1106	40-41	0.34013640355075125
1106	42-43	0.35179292586170163
1106	44-45	0.3301400206355538
1106	46-47	0.32688197035025013
1106	48-49	0.3128139017655158
1106	50-51	0.3577710909938858
1106	52-53	0.3769773059896906
1106	54-55	0.3915036993571235
1106	56-57	0.29901196518757445
1106	58-59	0.30052127523997996
1106	60-61	0.3374829223805733
1106	62-63	0.27121874574056193
1106	64-65	0.2941793263917205
1106	66-67	0.3399588836680465
1106	68-69	0.304732055959839
1106	70-71	0.29266334330763755
1106	72-73	0.2695008925665192
1106	74-75	0.3445601107993781
1106	76-77	0.4071689324752654
1106	78-79	0.30172431461596716
1106	80-81	0.3229695924536138
1106	82-83	0.33125698211766164
1106	84-85	0.2999510086981765
1106	86-87	0.2996825513991226
1106	88-89	0.3318938770925115
1106	90-91	0.3689068518802259
1106	92-93	0.3215843460477892
1106	94-95	0.3295261041818591
1106	96-97	0.33848579508251575
1106	98-99	0.4117467096054419
1106	100	0.332781902855217
1107	1	0.12134050074723035
1107	2	0.1519169003252152
1107	3	0.13453510856440687
1107	4	0.2206864613329742
1107	5	0.18186837877535567
1107	6	0.28166221486193876
1107	7	0.199930403107615
1107	8	0.24767794827810974
1107	9	0.31564744070881545
1107	10-11	0.2732069329240048
1107	12-13	0.3092518940537019
1107	14-15	0.33376345487668857
1107	16-17	0.36191759111594024
1107	18-19	0.3217776649795496
1107	20-21	0.2796492882900736
1107	22-23	0.4075209234213162
1107	24-25	0.39587085734037686
1107	26-27	0.414116592861717
1107	28-29	0.423159367380471
1107	30-31	0.4108686854917849
1107	32-33	0.35451237431501426
1107	34-35	0.34289004743682483
1107	36-37	0.30827257141771014
1107	38-39	0.42315701504466574
1107	40-41	0.4756689015558493
1107	42-43	0.43832397040879556
1107	44-45	0.3469577812292499
1107	46-47	0.3472889752856574
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2214	100	-0.004054105899257365
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2215	24-25	-0.6799241769771882
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2215	30-31	-0.7303206553989199
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2215	100	-0.4736114655491086
2216	1	-1.2631960291514446
2216	2	-1.2905206995705
2216	3	-1.3385572297596227
2216	4	-1.5899167492649369
2216	5	-1.4859740818977158
2216	6	-2.962461892575895
2216	7	-2.7916573547904626
2216	8	-3.6084367524589567
2216	9	-3.16993592936236
2216	10-11	-2.3254732588130054
2216	12-13	-2.078586477681924
2216	14-15	-2.3996265117485507
2216	16-17	-2.667583109187177
2216	18-19	-2.4554112974942868
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2216	26-27	-2.742416623726534
2216	28-29	-3.128804295529143
2216	30-31	-2.6063043417907394
2216	32-33	-2.8004212292288813
2216	34-35	-3.637762758200921
2216	36-37	-2.7567263123238135
2216	38-39	-2.6492374341900558
2216	40-41	-3.3114190740492475
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2216	52-53	-3.4155637613903274
2216	54-55	-2.8701786127956375
2216	56-57	-2.874590257513283
2216	58-59	-3.742642426674962
2216	60-61	-4.4599766535304965
2216	62-63	-3.5493960759754017
2216	64-65	-3.6324730674674193
2216	66-67	-3.2291488768437517
2216	68-69	-3.524099869638583
2216	70-71	-3.312879194388181
2216	72-73	-3.7849601129650203
2216	74-75	-4.209788207062761
2216	76-77	-4.924616480022461
2216	78-79	-3.8948817162787748
2216	80-81	-3.8042011567980616
2216	82-83	-4.018146272724959
2216	84-85	-4.101975097371454
2216	86-87	-3.715944926747053
2216	88-89	-3.7115854239058628
2216	90-91	-4.008749211472196
2216	92-93	-4.026267373225867
2216	94-95	-3.811972580567833
2216	96-97	-4.115793519491483
2216	98-99	-3.93174933483785
2216	100	-3.540828090865137
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	105271.0
3	17497.0
4	11529.0
5	9364.0
6	8111.0
7	8365.0
8	9396.0
9	10771.0
10	11920.0
11	12936.0
12	14158.0
13	15462.0
14	16674.0
15	17444.0
16	17875.0
17	18677.0
18	17611.0
19	16576.0
20	16266.0
21	17203.0
22	19439.0
23	23030.0
24	27474.0
25	34731.0
26	42189.0
27	49075.0
28	58490.0
29	71525.0
30	85197.0
31	101047.0
32	126941.0
33	165321.0
34	222347.0
35	317865.0
36	502927.0
37	806900.0
38	937459.0
39	281615.0
40	599.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.135927313792926	20.499084264178364	14.419695690319761	22.94529273170895
2	15.116072585108512	20.290670152259157	40.766571283899154	23.826685978733174
3	18.17719563305099	25.432237749309056	30.461483024551427	25.92908359308853
4	12.444508087705543	16.805432266364008	39.18331355341602	31.566746092514432
5	13.021253604172053	38.0843869692546	35.623445807525314	13.270913619048022
6	30.713332323696008	37.90040343882787	17.81153337137923	13.57473086609689
7	26.82481975315155	32.84604898342944	22.6294230310864	17.699708232332608
8	24.795989120675898	36.504027931260914	20.676062189638262	18.02392075842493
9	24.83036312771531	17.032342762340686	21.814088822125406	36.3232052878186
10-11	23.76928579184122	27.209150109743568	28.98193441199753	20.039629686417683
12-13	23.98880815362209	24.769043632726646	29.14921547201279	22.09293274163847
14-15	21.480601317641902	26.901433578848017	26.299856859975375	25.318108243534702
16-17	21.073147053534765	30.32982760908868	26.98537822007529	21.611647117301256
18-19	21.04773142652573	29.32565758483598	28.890384470446367	20.736226518191923
20-21	22.114631394743597	28.437316733672958	28.500637089689512	20.947414781893933
22-23	21.784168988355873	28.27130896166145	28.566822372345957	21.377699677636723
24-25	21.59370667201764	28.440773187293228	28.45218404237254	21.51333609831659
26-27	21.327364984096032	28.516327369105664	28.45177178694962	21.70453585984868
28-29	21.380820675039153	28.512918778835694	28.63483548898516	21.471425057139996
30-31	21.622382922389153	28.750885994799496	28.300808291038944	21.32592279177241
32-33	21.415693580220164	28.527558090787906	28.585613550827375	21.47113477816455
34-35	21.548507166090292	28.348388322994623	28.58316584959094	21.519938661324144
36-37	21.761278523305393	28.59181066708651	28.17493826871189	21.471972540896207
38-39	21.565265825408265	28.737688470614835	28.297099401321706	21.39994630265519
40-41	21.491971860856815	28.476933959636796	28.474790751657185	21.556303427849198
42-43	21.429754901077597	28.525184824844303	28.418095282843453	21.626964991234647
44-45	21.559851743253226	28.542238158743054	28.33467871326333	21.56323138474039
46-47	21.584423659137716	28.647332641710953	28.179463054529442	21.58878064462189
48-49	21.576682472759337	28.573292492160935	28.163593294649463	21.686431740430265
50-51	21.616952044994807	28.493681466396264	28.117374442445964	21.771992046162968
52-53	21.566174613296216	28.50057672164216	28.19986893492849	21.73337973013313
54-55	21.632565439958285	28.735185652508395	28.066216510769344	21.566032396763973
56-57	21.5688294829356	28.56458191462165	28.093499964025614	21.773088638417136
58-59	21.51913982931394	28.24839656517683	28.332392456879248	21.900071148629983
60-61	21.676731476877002	28.54914238820442	28.076740237930913	21.69738589698767
62-63	21.510844416559742	28.609290598145666	28.101018922030228	21.778846063264357
64-65	21.562174999246373	28.386354772706717	28.18793945754077	21.863530770506134
66-67	21.440325869609527	28.4181624357604	28.130264002474732	22.011247692155337
68-69	21.721849532021245	28.53445160412093	28.04237414772851	21.701324716129314
70-71	21.66013449487175	28.376270052381802	28.08429180585042	21.879303646896027
72-73	21.53095353942806	28.46354734584698	28.21861889557052	21.78688021915444
74-75	21.597062166026102	28.475539383411423	28.096935050021532	21.830463400540943
76-77	21.631904812539396	28.444630026267596	27.984655586160073	21.938809575032934
78-79	21.656207037655253	28.479241183795672	28.094922678819028	21.769629099730047
80-81	21.633210357305614	28.38895123390135	28.124884452383785	21.85295395640926
82-83	21.482484399319944	28.471049749077338	28.236871748361864	21.809594103240855
84-85	21.53979126703477	28.32268237050749	28.1318246530047	22.00570170945304
86-87	21.66473292251525	28.4696674968833	28.064705791611814	21.800893788989633
88-89	21.585081903489687	28.340089982147	28.09980865811153	21.975019456251783
90-91	21.52143086431645	28.283375910169667	28.177300827192525	22.017892398321358
92-93	21.590336640602757	28.400596217565244	28.061120072273365	21.947947069558637
94-95	21.541929552750887	28.233102880284328	28.15975246678889	22.06521510017589
96-97	21.57304550656809	28.249052275140045	28.134084496961233	22.04381772133063
98-99	21.494524609532178	28.34653826439858	28.07175044151818	22.087186684551067
100	21.54933619822771	28.185964795797403	28.07450514765107	22.190193858323816
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1199.0
1	1017.5
2	967.0
3	1122.5
4	1045.0
5	847.5
6	672.0
7	589.0
8	567.0
9	551.0
10	522.5
11	539.5
12	613.0
13	669.5
14	727.0
15	846.0
16	1007.5
17	1271.5
18	1737.0
19	2434.5
20	3573.0
21	5117.0
22	7451.5
23	11218.0
24	16317.5
25	22660.5
26	31066.5
27	41148.0
28	52588.0
29	65497.5
30	78380.5
31	90065.0
32	100220.5
33	110411.0
34	120552.0
35	128690.0
36	135693.0
37	143359.0
38	150092.0
39	153864.0
40	156503.5
41	157795.5
42	156873.5
43	156179.5
44	153351.0
45	149219.0
46	145581.5
47	140324.0
48	134360.5
49	130186.0
50	127325.0
51	121784.5
52	113136.0
53	105483.5
54	99383.0
55	93434.0
56	86172.0
57	78123.0
58	71309.0
59	64972.5
60	57975.5
61	49769.0
62	42469.0
63	35865.5
64	29531.0
65	24064.0
66	19388.5
67	15253.5
68	11586.0
69	8880.0
70	6755.5
71	5057.0
72	3773.0
73	2631.5
74	1848.0
75	1341.0
76	941.5
77	613.0
78	427.0
79	322.0
80	217.5
81	142.5
82	107.5
83	92.5
84	80.5
85	57.5
86	39.0
87	32.5
88	27.0
89	23.0
90	19.0
91	13.0
92	13.0
93	13.0
94	12.5
95	11.0
96	7.5
97	9.5
98	11.5
99	11.5
100	21.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00986514418532156
2	0.0288890976500944
3	0.030419489946146672
4	0.03267976164493156
5	0.03538737878410096
6	0.03597599120565953
7	0.03373926400373698
8	0.03355090802883824
9	0.03397470897236041
10-11	0.03461041038764366
12-13	0.03549332901998151
14-15	0.033774580749030496
16-17	0.03099633011927407
18-19	0.03383344199118635
20-21	0.033127107085316077
22-23	0.03404534246294744
24-25	0.031078735858292267
26-27	0.031161141597310465
28-29	0.031679120528282
30-31	0.03163203153455732
32-33	0.031478992304952096
34-35	0.03160848703769498
36-37	0.029513026816946484
38-39	0.029595432555964682
40-41	0.0307373406537883
42-43	0.030548984678889557
44-45	0.02977201628243225
46-47	0.028771375165782688
48-49	0.029077453624993144
50-51	0.030042777996349187
52-53	0.030478351188302527
54-55	0.029819105276156936
56-57	0.02826516848324232
58-59	0.029100998121855487
60-61	0.028547702445590434
62-63	0.028747830668920348
64-65	0.026675914945034195
66-67	0.027346933105610958
68-69	0.02819453499265529
70-71	0.02923049285459837
72-73	0.026817181926208248
74-75	0.027205666124436905
76-77	0.027582378074234384
78-79	0.028006179017756552
80-81	0.02806504025991241
82-83	0.027029082397969334
84-85	0.027829595291288985
86-87	0.02817099049579295
88-89	0.028547702445590434
90-91	0.028912642146956744
92-93	0.027688328310114925
94-95	0.028736058420489173
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4247277.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.10926659230296
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.71881557909228	52.701524315408456
2	12.484815393220549	16.5072397837221
3	3.5928940871258086	7.125707791311273
4	1.4825495370860537	3.9204105033406904
5	0.7765436859779427	2.56683667784427
6	0.4474461408438608	1.7748201726446362
7	0.30800759497798064	1.4253509346197377
8	0.20940315453240477	1.107479117460157
9	0.1514938735051848	0.9013633983589395
>10	0.7785439439275652	9.043695025166743
>50	0.03874924482473495	1.7339668594065056
>100	0.01059533826629503	1.1114541373534519
>500	1.0681999451763499E-4	0.04608994753720784
>1k	3.5606624608392234E-5	0.034061335825819085
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.3544496862342624E-5	0.0
10-11	0.0	1.1772248431171312E-5	0.0	2.3544496862342624E-5	0.0
12-13	0.0	2.3544496862342624E-5	0.0	2.3544496862342624E-5	0.0
14-15	0.0	9.41779874493705E-5	0.0	2.3544496862342624E-5	0.0
16-17	0.0	1.6481147803639837E-4	0.0	2.3544496862342624E-5	0.0
18-19	0.0	1.6481147803639837E-4	0.0	2.3544496862342624E-5	0.0
20-21	0.0	1.6481147803639837E-4	0.0	5.886124215585657E-5	0.0
22-23	2.3544496862342624E-5	1.6481147803639837E-4	0.0	1.4126698117405577E-4	0.0
24-25	2.3544496862342624E-5	1.7658372646756968E-4	0.0	3.178507076416255E-4	0.0
26-27	2.3544496862342624E-5	1.88355974898741E-4	0.0	6.474736637144222E-4	0.0
28-29	4.708899372468525E-5	2.1190047176108362E-4	0.0	0.0023544496862342623	0.0
30-31	4.708899372468525E-5	2.1190047176108362E-4	0.0	0.007734367219279553	0.0
32-33	4.708899372468525E-5	2.1190047176108362E-4	0.0	0.019530160147313208	0.0
34-35	4.708899372468525E-5	2.2367272019225494E-4	0.0	0.03849525236993019	0.0
36-37	4.708899372468525E-5	2.707617139169402E-4	0.0	0.06802005143530784	0.0
38-39	4.708899372468525E-5	3.178507076416255E-4	0.0	0.10691556025189786	0.0
40-41	4.708899372468525E-5	3.5316745293513936E-4	0.0	0.14644677048377114	0.0
42-43	4.708899372468525E-5	3.5316745293513936E-4	0.0	0.18513037882860006	0.0
44-45	4.708899372468525E-5	3.5316745293513936E-4	0.0	0.23216051131112947	0.0
46-47	4.708899372468525E-5	3.5316745293513936E-4	0.0	0.28917351046329215	0.0
48-49	4.708899372468525E-5	3.5316745293513936E-4	0.0	0.34706942824779263	0.0
50-51	4.708899372468525E-5	3.76711949797482E-4	0.0	0.4059777593973739	0.0
52-53	4.708899372468525E-5	3.76711949797482E-4	0.0	0.46274354133248197	0.0
54-55	4.708899372468525E-5	3.76711949797482E-4	0.0	0.5236413824669311	0.0
56-57	4.708899372468525E-5	3.76711949797482E-4	0.0	0.5975357858693935	0.0
58-59	4.708899372468525E-5	3.76711949797482E-4	0.0	0.6769984627797998	0.0
60-61	4.708899372468525E-5	3.76711949797482E-4	0.0	0.7569438018758843	0.0
62-63	4.708899372468525E-5	3.76711949797482E-4	0.0	0.836571290264327	0.0
64-65	4.708899372468525E-5	3.76711949797482E-4	0.0	0.9188828512950769	0.0
66-67	4.708899372468525E-5	3.76711949797482E-4	0.0	1.0174636596577054	0.0
68-69	4.708899372468525E-5	3.76711949797482E-4	0.0	1.1225192046574781	0.0
70-71	4.708899372468525E-5	3.76711949797482E-4	0.0	1.2340141695491016	0.0
72-73	4.708899372468525E-5	4.1202869509099593E-4	0.0	1.349970816596139	0.0
74-75	4.708899372468525E-5	4.2380094352216724E-4	0.0	1.4679405181249068	0.0
76-77	4.708899372468525E-5	4.3557319195333856E-4	0.0	1.5935974978792293	0.0
78-79	4.708899372468525E-5	4.4734544038450987E-4	0.0	1.7367480388022727	0.0
80-81	4.708899372468525E-5	4.4734544038450987E-4	0.0	1.88617318813913	0.0
82-83	4.708899372468525E-5	4.591176888156812E-4	0.0	2.0416845899149028	0.0
84-85	4.708899372468525E-5	4.944344341091952E-4	0.0	2.19211038036841	0.0
86-87	4.708899372468525E-5	5.062066825403665E-4	0.0	2.3520246030574414	0.0
88	4.708899372468525E-5	5.179789309715378E-4	0.0	2.480836545391318	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4295	0.0	43.889812	1
GTATCAA	7220	0.0	36.59162	1
TCAACGC	7795	0.0	32.503075	4
ATCAACG	8135	0.0	31.202404	3
CAACGCA	8415	0.0	30.164532	5
TATCAAC	9095	0.0	28.270693	2
AACGCAG	8965	0.0	28.260183	6
GTACATG	12970	0.0	25.842356	1
TACATGG	13160	0.0	24.574482	2
ACGCAGA	10465	0.0	23.984652	7
CGCAGAG	10440	0.0	23.63688	8
ACATGGG	13355	0.0	23.40613	3
CATGGGG	9115	0.0	20.370058	4
GCAGAGT	12100	0.0	19.228756	9
GAGTACA	9735	0.0	18.253174	1
AGAGTAC	10400	0.0	17.987926	10-11
AGTACAT	10000	0.0	17.06312	2
CAGAGTA	11505	0.0	16.750532	10-11
GTGGTAT	2605	0.0	16.602085	1
CATGGGT	3235	0.0	15.25692	4
>>END_MODULE