FastQCFastQC Report
Wed 25 May 2016
SRR2049353_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049353_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4247277
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98370.23160721563486442No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92930.21879900934175No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54230.12768180648448405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65100.056.2526631
GTATCAA125000.042.120611
TCAACGC154950.033.4843144
ATCAACG157250.033.024453
CAACGCA161950.032.1240735
TATCAAC162650.032.0725022
AACGCAG169000.030.9774596
GTGGTAT30350.029.8940851
TGGTATC30600.027.3377322
ACGCAGA192850.026.8296787
CGCAGAG195300.026.3245128
GTACATG125200.024.068021
GCAGAGT213550.023.5241589
TACATGG127850.022.2759282
ACATGGG129150.021.5058693
GAGTACT144550.020.71939312-13
AGAGTAC197450.020.59445210-11
CAGAGTA206650.020.28078310-11
CATGGGG89450.020.017444
AGTACTT151350.019.61763212-13
Produced by FastQC (version 0.11.5)