##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049353_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4247277
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80434735007865	31.0	31.0	34.0	30.0	34.0
2	31.86488119329161	31.0	31.0	34.0	30.0	34.0
3	31.888225326485653	33.0	31.0	34.0	30.0	34.0
4	35.6474955600965	37.0	35.0	37.0	33.0	37.0
5	35.76321370139033	37.0	35.0	37.0	35.0	37.0
6	35.83981336748227	37.0	35.0	37.0	35.0	37.0
7	35.77860850610874	37.0	35.0	37.0	33.0	37.0
8	35.776339287501145	37.0	35.0	37.0	33.0	37.0
9	37.49464233201649	39.0	37.0	39.0	35.0	39.0
10-11	37.463759721817055	39.0	37.0	39.0	34.5	39.0
12-13	37.422606531196344	39.0	37.0	39.0	34.0	39.0
14-15	38.71767370011421	40.0	38.0	41.0	34.0	41.0
16-17	38.75082988465316	40.0	38.0	41.0	35.0	41.0
18-19	38.815874735742455	40.0	38.0	41.0	35.0	41.0
20-21	38.845442738959576	40.0	38.0	41.0	35.0	41.0
22-23	38.817808092102304	40.0	38.0	41.0	35.0	41.0
24-25	38.806397016253	40.0	38.0	41.0	35.0	41.0
26-27	38.70131557230668	40.0	38.0	41.0	34.5	41.0
28-29	38.621463822585625	40.0	38.0	41.0	34.0	41.0
30-31	38.52091940318468	40.0	38.0	41.0	34.0	41.0
32-33	38.39440622780195	40.0	38.0	41.0	34.0	41.0
34-35	38.29960042634375	40.0	38.0	41.0	34.0	41.0
36-37	38.19622760653473	40.0	38.0	41.0	34.0	41.0
38-39	38.10667258104428	40.0	38.0	41.0	33.5	41.0
40-41	37.997083307728694	40.0	38.0	41.0	33.0	41.0
42-43	37.86628938964894	40.0	37.0	41.0	33.0	41.0
44-45	37.69425104602313	40.0	37.0	41.0	33.0	41.0
46-47	37.464356810257485	40.0	37.0	41.0	33.0	41.0
48-49	37.25933780631685	40.0	37.0	41.0	32.0	41.0
50-51	37.113659999100605	40.0	37.0	41.0	31.5	41.0
52-53	36.975647338282855	40.0	36.0	41.0	31.0	41.0
54-55	36.844714978561555	40.0	36.0	41.0	31.0	41.0
56-57	36.70333580785996	39.0	36.0	41.0	31.0	41.0
58-59	36.51602732762662	39.0	35.0	41.0	31.0	41.0
60-61	36.34000996403107	39.0	35.0	41.0	31.0	41.0
62-63	36.14611408674311	39.0	35.0	41.0	30.5	41.0
64-65	35.919446624272446	38.0	35.0	40.0	30.0	41.0
66-67	35.66071826725688	38.0	35.0	40.0	30.0	41.0
68-69	35.378613756531536	37.0	35.0	40.0	29.5	41.0
70-71	35.05250846601246	37.0	34.0	39.5	29.0	41.0
72-73	34.7594004346785	36.0	34.0	39.0	29.0	41.0
74-75	34.45591375368265	36.0	34.0	39.0	29.0	41.0
76-77	34.12828901905857	35.5	34.0	38.5	29.0	40.0
78-79	33.79417907049623	35.0	34.0	37.0	29.0	39.0
80-81	33.480123382581354	35.0	34.0	37.0	29.0	39.0
82-83	33.18021181571157	35.0	34.0	36.5	28.5	39.0
84-85	32.91183150992977	35.0	34.0	36.0	28.0	37.5
86-87	32.67838464503257	35.0	33.0	36.0	27.5	37.0
88-89	32.448301700124574	35.0	33.0	35.0	27.0	37.0
90-91	32.25732534044754	35.0	33.0	35.0	27.0	36.0
92-93	32.08271558459691	35.0	33.0	35.0	27.0	36.0
94-95	31.923707471869626	35.0	33.0	35.0	26.5	36.0
96-97	31.73069027520456	35.0	33.0	35.0	26.0	36.0
98-99	31.58887470725361	35.0	33.0	35.0	25.5	35.0
100	31.47842252812802	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.20727683537240438
1101	2	0.22779584562611888
1101	3	0.37957749009441244
1101	4	0.2678802830217748
1101	5	0.2075768641531397
1101	6	0.21508745539385643
1101	7	0.09328669231808817
1101	8	0.1234217374767681
1101	9	0.23208751835220198
1101	10-11	0.22321898035939824
1101	12-13	0.22366812261105196
1101	14-15	0.3324011333746526
1101	16-17	0.24497450369753437
1101	18-19	0.317763902077175
1101	20-21	0.2984802240552682
1101	22-23	0.32914773407084397
1101	24-25	0.2986098547167586
1101	26-27	0.358855068884985
1101	28-29	0.3633478284821763
1101	30-31	0.3711654178843702
1101	32-33	0.3521144448793905
1101	34-35	0.331194990227921
1101	36-37	0.38499363598744907
1101	38-39	0.34758866669623245
1101	40-41	0.3573535077570469
1101	42-43	0.3309837324386109
1101	44-45	0.32215872718850846
1101	46-47	0.3376243076519998
1101	48-49	0.4267629820619021
1101	50-51	0.3210917480555011
1101	52-53	0.40063977984168986
1101	54-55	0.33959804456898723
1101	56-57	0.3810054178366613
1101	58-59	0.3897802805290169
1101	60-61	0.38845140531193323
1101	62-63	0.3214523840200556
1101	64-65	0.29001256192092484
1101	66-67	0.2628329678945107
1101	68-69	0.3270764142193059
1101	70-71	0.33481464513376835
1101	72-73	0.29624780535295514
1101	74-75	0.3502096638908512
1101	76-77	0.3123448583344981
1101	78-79	0.37366969022021124
1101	80-81	0.3981447716796822
1101	82-83	0.3590535924181424
1101	84-85	0.3740943517370141
1101	86-87	0.36135131622786787
1101	88-89	0.3769296232569843
1101	90-91	0.4186551121880342
1101	92-93	0.4149623456730893
1101	94-95	0.4421459924411053
1101	96-97	0.5318477841894058
1101	98-99	0.5556459672302161
1101	100	0.5704131918390125
1102	1	0.22906466081385446
1102	2	0.17321520521885247
1102	3	0.3636797125112565
1102	4	0.2675299333357728
1102	5	0.2018405920440003
1102	6	0.2102012917502094
1102	7	0.19991908404580272
1102	8	0.22341132945373232
1102	9	0.19345908986071692
1102	10-11	0.2552357959534177
1102	12-13	0.24412395680417376
1102	14-15	0.30626423053186613
1102	16-17	0.2888521113011677
1102	18-19	0.28757525138742324
1102	20-21	0.25863457789009203
1102	22-23	0.27108093937350475
1102	24-25	0.30256388623781305
1102	26-27	0.33686219670605055
1102	28-29	0.2852620760484825
1102	30-31	0.27802867172016477
1102	32-33	0.28024556959466906
1102	34-35	0.29172629065147504
1102	36-37	0.37592378202603527
1102	38-39	0.36397656416269797
1102	40-41	0.3767055704870401
1102	42-43	0.4083675573783836
1102	44-45	0.3786633011983014
1102	46-47	0.35160591733277613
1102	48-49	0.28122948156339334
1102	50-51	0.3785077745998038
1102	52-53	0.4189222476323309
1102	54-55	0.3616526577856405
1102	56-57	0.4144003722900891
1102	58-59	0.3671810014489836
1102	60-61	0.39411714104565476
1102	62-63	0.41510829080883127
1102	64-65	0.44244140869336945
1102	66-67	0.43279378832460225
1102	68-69	0.4209676366087365
1102	70-71	0.47299303517511504
1102	72-73	0.49601623614437784
1102	74-75	0.3765660904232959
1102	76-77	0.3945950108883238
1102	78-79	0.4357853547414692
1102	80-81	0.4449973619438552
1102	82-83	0.5220797666732508
1102	84-85	0.4643638302156674
1102	86-87	0.4429467557522315
1102	88-89	0.45296673189081815
1102	90-91	0.4644506988637289
1102	92-93	0.5462617643992616
1102	94-95	0.49560240509205045
1102	96-97	0.5591700107520943
1102	98-99	0.6245760460322209
1102	100	0.611951847472266
1103	1	0.13703713587518251
1103	2	0.16079381780720325
1103	3	0.3302496853750725
1103	4	0.21230959307061426
1103	5	0.21299601516356859
1103	6	0.17638856685213966
1103	7	0.1659691940651271
1103	8	0.12726686517793695
1103	9	0.20334045762400166
1103	10-11	0.20737157358607305
1103	12-13	0.2172276076455404
1103	14-15	0.2834198528160954
1103	16-17	0.23014435479528572
1103	18-19	0.2532824965763467
1103	20-21	0.3249824055590409
1103	22-23	0.2740813836638907
1103	24-25	0.2886813435945541
1103	26-27	0.2682289067165655
1103	28-29	0.34194247039449976
1103	30-31	0.2826677958935875
1103	32-33	0.3270710597025115
1103	34-35	0.29073774453431867
1103	36-37	0.2893957270987215
1103	38-39	0.3177973897001962
1103	40-41	0.2635387758315417
1103	42-43	0.3029969938190078
1103	44-45	0.32344213507008135
1103	46-47	0.2988200418413314
1103	48-49	0.22332235740529427
1103	50-51	0.22746582267126314
1103	52-53	0.25010950597761195
1103	54-55	0.1687898381886015
1103	56-57	0.1746856828433252
1103	58-59	0.24583026291195864
1103	60-61	0.23935040307548405
1103	62-63	0.2252602783380624
1103	64-65	0.29945644514624803
1103	66-67	0.25234986658099245
1103	68-69	0.2943012484214975
1103	70-71	0.3465021488732347
1103	72-73	0.29185302453768003
1103	74-75	0.23313444268046624
1103	76-77	0.183963400393111
1103	78-79	0.1996501473133918
1103	80-81	0.25089283927834316
1103	82-83	0.2239343439175414
1103	84-85	0.24925550331369095
1103	86-87	0.20747345961544994
1103	88-89	0.262891523294563
1103	90-91	0.29205529142838316
1103	92-93	0.29489140622843735
1103	94-95	0.2093750720408778
1103	96-97	0.26412503353154904
1103	98-99	0.3055996638007734
1103	100	0.3511068052359505
1104	1	0.1490780985804605
1104	2	0.21400121393421045
1104	3	0.2948372291361636
1104	4	0.17022505739465288
1104	5	0.16659690146975237
1104	6	0.163268054773134
1104	7	0.17190543612395004
1104	8	0.13262929332785944
1104	9	0.20471320769252088
1104	10-11	0.18906792426793828
1104	12-13	0.1858811612619462
1104	14-15	0.2580429384585514
1104	16-17	0.2989686877879123
1104	18-19	0.2552044158342568
1104	20-21	0.2614947955131015
1104	22-23	0.235772423627715
1104	24-25	0.24673395612848026
1104	26-27	0.2673945365682826
1104	28-29	0.3150752075454051
1104	30-31	0.33012086902786564
1104	32-33	0.35245259638584514
1104	34-35	0.2873128431961973
1104	36-37	0.30706351268329257
1104	38-39	0.28851063324222537
1104	40-41	0.265212956870684
1104	42-43	0.3068594945429055
1104	44-45	0.32773420086285654
1104	46-47	0.3479735429868427
1104	48-49	0.28594235344370134
1104	50-51	0.25807841432011003
1104	52-53	0.24902978369893702
1104	54-55	0.19910086054286324
1104	56-57	0.20223690005731498
1104	58-59	0.28293455954936064
1104	60-61	0.26303192671947784
1104	62-63	0.23803989100883172
1104	64-65	0.16026720754366863
1104	66-67	0.22303765851371793
1104	68-69	0.22784321194675528
1104	70-71	0.2514297141464752
1104	72-73	0.27170134401217894
1104	74-75	0.2763626985222558
1104	76-77	0.25435457502915426
1104	78-79	0.25685836812786533
1104	80-81	0.29430928487257546
1104	82-83	0.3079263605960918
1104	84-85	0.31848202475741516
1104	86-87	0.2797357309797661
1104	88-89	0.32583517435373466
1104	90-91	0.23331273510194706
1104	92-93	0.36423629840398775
1104	94-95	0.3505111156878016
1104	96-97	0.36951644746804035
1104	98-99	0.397529159062163
1104	100	0.34265350506594316
1105	1	0.18920318279193538
1105	2	0.16866753265710344
1105	3	0.2403957765754754
1105	4	0.1849383204570927
1105	5	0.13894878267294786
1105	6	0.13793681109255118
1105	7	0.11945429381636785
1105	8	0.13453024880465136
1105	9	0.19470063854309672
1105	10-11	0.2151282080213761
1105	12-13	0.14100167225096527
1105	14-15	0.2146753741443348
1105	16-17	0.2025085916460938
1105	18-19	0.19347937943905436
1105	20-21	0.22739234797862906
1105	22-23	0.2370118285093099
1105	24-25	0.25823259701581947
1105	26-27	0.2973919107274696
1105	28-29	0.253640913904043
1105	30-31	0.25121985525102986
1105	32-33	0.2732185238747178
1105	34-35	0.2968928291877049
1105	36-37	0.24433399805661793
1105	38-39	0.2884401251726558
1105	40-41	0.27668268165241017
1105	42-43	0.25929593151819574
1105	44-45	0.24228607284764792
1105	46-47	0.2405340594614529
1105	48-49	0.247100482682157
1105	50-51	0.22960669889397423
1105	52-53	0.26304473840479403
1105	54-55	0.255720656014212
1105	56-57	0.16199110558520147
1105	58-59	0.24502551166204256
1105	60-61	0.1925852314772527
1105	62-63	0.19986704492064433
1105	64-65	0.18664653752720284
1105	66-67	0.240471263247791
1105	68-69	0.24828624143768963
1105	70-71	0.22900917278662547
1105	72-73	0.246517610341904
1105	74-75	0.23903733046957143
1105	76-77	0.2821963292574452
1105	78-79	0.27697926596447786
1105	80-81	0.3127363070935729
1105	82-83	0.2371160308453355
1105	84-85	0.20264985321067286
1105	86-87	0.18253493252990438
1105	88-89	0.1849745985253648
1105	90-91	0.1873163670806477
1105	92-93	0.28477523000824334
1105	94-95	0.2979995681256753
1105	96-97	0.29504220431089934
1105	98-99	0.41825824190767236
1105	100	0.4055233118152408
1106	1	0.15161534140566602
1106	2	0.15890743327057777
1106	3	0.24974399489368437
1106	4	0.14811074542611635
1106	5	0.12230759846985251
1106	6	0.1718820492269728
1106	7	0.2008399951478168
1106	8	0.12626860614278002
1106	9	0.20038350004375616
1106	10-11	0.16447004965825585
1106	12-13	0.11240127074341899
1106	14-15	0.24898508462083413
1106	16-17	0.17325116726798484
1106	18-19	0.16746723428074972
1106	20-21	0.2397939702269909
1106	22-23	0.18120092738308813
1106	24-25	0.25460233227293827
1106	26-27	0.23150596676781987
1106	28-29	0.2575845265027539
1106	30-31	0.21712060979292858
1106	32-33	0.21309315544345253
1106	34-35	0.1939163218180724
1106	36-37	0.18298274575478501
1106	38-39	0.1826761202163567
1106	40-41	0.16952025041664598
1106	42-43	0.22515313516704083
1106	44-45	0.2069224963315932
1106	46-47	0.21084855767287536
1106	48-49	0.2772014282971824
1106	50-51	0.21022484660902307
1106	52-53	0.23882131640009874
1106	54-55	0.2349200828429332
1106	56-57	0.20068329270361573
1106	58-59	0.2447562064910187
1106	60-61	0.21778196403023742
1106	62-63	0.20134291201018328
1106	64-65	0.15692016237851902
1106	66-67	0.17112503963698344
1106	68-69	0.20704598282166842
1106	70-71	0.2834087080455916
1106	72-73	0.25518464242077243
1106	74-75	0.22734569160061824
1106	76-77	0.221256302301569
1106	78-79	0.24884413225362323
1106	80-81	0.2378699504130921
1106	82-83	0.2972427435074252
1106	84-85	0.2456097983450718
1106	86-87	0.23974526537654128
1106	88-89	0.2533079606011981
1106	90-91	0.1792091907480966
1106	92-93	0.32089038455839614
1106	94-95	0.26346789563214656
1106	96-97	0.2382787243914919
1106	98-99	0.1989608510334797
1106	100	0.2284585825545875
1107	1	0.15965855298663456
1107	2	0.07442013596165964
1107	3	0.26158431585976416
1107	4	0.213698858014979
1107	5	0.14210515734422557
1107	6	0.13023929891213015
1107	7	0.1288911043053247
1107	8	0.15084764381440152
1107	9	0.19257396935165616
1107	10-11	0.13113626735370332
1107	12-13	0.15442696329449035
1107	14-15	0.2478199611200722
1107	16-17	0.15764946034845195
1107	18-19	0.16392721488519157
1107	20-21	0.1841451092410864
1107	22-23	0.21655749774024002
1107	24-25	0.21956788797758264
1107	26-27	0.2469582288661698
1107	28-29	0.2554967779259414
1107	30-31	0.21341950038502944
1107	32-33	0.2525671588870608
1107	34-35	0.18437337366233209
1107	36-37	0.24969402030293253
1107	38-39	0.2340432742930858
1107	40-41	0.23909566998457166
1107	42-43	0.2627100206847359
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2216	82-83	-1.0558144941259613
2216	84-85	-1.181237909415799
2216	86-87	-0.9604508924011306
2216	88-89	-1.3317830729751563
2216	90-91	-1.1418394121153952
2216	92-93	-1.2640844912045353
2216	94-95	-1.1744089419635628
2216	96-97	-1.271021642783925
2216	98-99	-1.491286127792094
2216	100	-1.4820261447763485
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	222.0
3	20.0
4	33.0
5	59.0
6	156.0
7	474.0
8	1152.0
9	2013.0
10	3034.0
11	4321.0
12	6226.0
13	8633.0
14	10587.0
15	13511.0
16	17154.0
17	24155.0
18	23505.0
19	12900.0
20	9910.0
21	9810.0
22	10304.0
23	11471.0
24	13662.0
25	17227.0
26	22034.0
27	29200.0
28	38544.0
29	50971.0
30	67076.0
31	86184.0
32	111352.0
33	146807.0
34	199894.0
35	288060.0
36	469482.0
37	846083.0
38	1197233.0
39	491733.0
40	2085.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.19742534050364	20.999057195473313	14.845697309109168	22.957820154913875
2	15.395840676273293	20.751884089500166	39.968455083103834	23.88382015112271
3	17.801970533120397	25.738914603403547	30.922777111076112	25.53633775239995
4	12.617969729304146	17.34733038305022	38.42660566443057	31.608094223215065
5	12.602356757046925	38.065117956752054	35.70360492145909	13.628920364741928
6	30.446919284991303	38.42784447541331	17.305864439733977	13.819371799861417
7	26.384363440387805	33.33594677248505	22.44320302160655	17.83648676552059
8	24.931508848990784	36.54133578699134	20.188549616882774	18.338605747135098
9	24.785927548403368	16.670610369891108	21.72700297155095	36.816459110154575
10-11	23.795646010373233	27.187666356585645	28.705933236753804	20.310754396287315
12-13	24.313095101954723	24.707659762354346	29.226026946333107	21.753218189357824
14-15	21.57018000499615	26.62449605876338	26.91966206065024	24.88566187559023
16-17	20.625438369100955	30.10039138017134	27.643605538324906	21.630564712402794
18-19	20.509218203528356	28.430861148517444	30.50859309691589	20.551327551038305
20-21	21.5523819636741	27.50394194109962	30.41468019597516	20.52899589925112
22-23	21.23534678807151	27.34282459090848	30.490370653950755	20.931457967069253
24-25	21.084352880279226	27.524277062174402	30.34518286508947	21.046187192456898
26-27	20.82560042945208	27.616017102740642	30.390750770053014	21.167631697754263
28-29	20.8462936466584	27.593347806974894	30.56724086417105	20.993117682195656
30-31	21.12183196947879	27.815098430146755	30.22474875661483	20.838320843759625
32-33	20.861162480703786	27.663272212118343	30.51157207905642	20.96399322812145
34-35	21.06537969231798	27.494714551649537	30.437531174403727	21.00237458162876
36-37	21.27967932380173	27.73352325903079	29.9611515618838	21.02564585528368
38-39	21.157253779022813	27.854274118243705	30.052572692713564	20.935899410019914
40-41	21.0423135032761	27.682244583759978	30.14821510178413	21.127226811179785
42-43	21.061240855710437	27.755706657088712	29.96363886364709	21.219413623553756
44-45	21.205844799344895	27.840034864805872	29.752242797302642	21.201877538546587
46-47	21.336093537731298	27.986553715680895	29.379813386010305	21.2975393605775
48-49	21.41528632892023	28.016903685323136	29.118960680225353	21.448849305531276
50-51	21.47498717111223	28.081249269380272	28.87803533132788	21.56572822817962
52-53	21.412262550684282	28.14542167711637	28.945716267837874	21.496599504361473
54-55	21.441316654770727	28.379549724957226	28.803730226757395	21.375403393514645
56-57	21.408069081892773	28.1853500936724	28.799170945710976	21.607409878723853
58-59	21.313353761350083	27.857131337141976	29.09364282457573	21.735872076932214
60-61	21.445510895066274	28.15460249551094	28.8697919002728	21.530094709149992
62-63	21.262758452060616	28.192010163792503	28.929878708104933	21.615352676041947
64-65	21.279084961934238	27.98614634628719	29.01581138876757	21.718957303011003
66-67	21.229585759528767	28.013672005140304	28.932155370372943	21.824586864957986
68-69	21.51865980052592	28.094751894681192	28.804757749100972	21.58183055569192
70-71	21.538664384408282	28.02220493000773	28.748309202061094	21.69082148352289
72-73	21.339147630638923	28.160497020235415	28.769175873742697	21.731179475382962
74-75	21.48751146187007	28.181325989888244	28.59587809432232	21.73528445391937
76-77	21.521879758596597	28.1662182389889	28.528111441586578	21.783790560827924
78-79	21.522418847835933	28.242957979030308	28.576347860360745	21.658275312773014
80-81	21.451628853163054	28.16466768381695	28.574882980627052	21.80882048239294
82-83	21.411476212675584	28.20515537221995	28.688153662866533	21.695214752237934
84-85	21.460490266898603	28.103912359235217	28.52037928439643	21.915218089469754
86-87	21.49664132454011	28.25536057788525	28.446568453172787	21.801429644401853
88-89	21.38312869767193	28.14356340938964	28.54885562369877	21.924452269239666
90-91	21.29200561036236	28.066280351888484	28.61141177088305	22.030302266866112
92-93	21.373443873004167	28.191277330302604	28.479716931043146	21.955561865650083
94-95	21.288807547340404	28.0320721469083	28.561761498166284	22.117358807585013
96-97	21.322499649174357	28.07669378148601	28.497543278698718	22.103263290640914
98-99	21.246199058495502	28.126714021687775	28.469827390842095	22.157259528974627
100	21.341756719625977	28.031563212009353	28.42191238840061	22.20476767996406
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	357.0
1	292.5
2	390.0
3	578.0
4	401.0
5	254.0
6	664.5
7	1853.5
8	3180.0
9	3646.5
10	2895.5
11	1987.5
12	1902.5
13	2198.5
14	2589.0
15	2942.0
16	3306.0
17	3833.0
18	4550.5
19	5374.5
20	6464.5
21	8069.5
22	10375.5
23	13932.5
24	18722.5
25	24707.0
26	32746.5
27	42552.5
28	53946.5
29	66920.5
30	79527.5
31	90328.5
32	100240.0
33	110207.0
34	120113.0
35	128231.0
36	135032.0
37	142538.5
38	149212.0
39	153475.5
40	156185.5
41	157063.5
42	156083.0
43	155506.0
44	152852.0
45	148501.0
46	145046.0
47	139919.0
48	133814.5
49	129494.0
50	125987.5
51	120144.0
52	111490.5
53	103708.0
54	97321.5
55	91103.5
56	83518.5
57	75486.5
58	69506.5
59	63874.5
60	56627.0
61	48358.5
62	41160.5
63	34577.0
64	28111.0
65	22520.5
66	17999.5
67	13791.0
68	10300.5
69	7947.0
70	5846.0
71	4154.0
72	2962.0
73	1997.0
74	1421.5
75	984.0
76	595.5
77	367.5
78	234.0
79	144.0
80	98.0
81	49.5
82	22.5
83	15.0
84	10.0
85	6.0
86	3.5
87	1.5
88	1.5
89	1.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033762808500599324
2	0.0
3	0.0
4	2.3544496862342624E-5
5	0.0
6	0.0
7	0.0
8	3.060784592104541E-4
9	0.0
10-11	0.0
12-13	0.04328655748141692
14-15	1.4126698117405577E-4
16-17	0.0
18-19	3.531674529351394E-5
20-21	9.41779874493705E-5
22-23	0.0
24-25	1.5303922960522705E-4
26-27	1.0595023588054181E-4
28-29	3.76711949797482E-4
30-31	2.3544496862342624E-5
32-33	1.5303922960522705E-4
34-35	5.886124215585656E-5
36-37	4.708899372468525E-5
38-39	3.531674529351394E-4
40-41	9.41779874493705E-5
42-43	5.29751179402709E-4
44-45	3.296229560727968E-4
46-47	1.648114780363984E-4
48-49	8.829186323378485E-4
50-51	6.239291668520796E-4
52-53	6.710181605767648E-4
54-55	6.710181605767648E-4
56-57	6.592459121455936E-4
58-59	1.2949473274288445E-4
60-61	2.472172170545976E-4
62-63	0.0010241856135119042
64-65	5.179789309715378E-4
66-67	0.0013655808180158724
68-69	0.0013773530664470436
70-71	5.179789309715378E-4
72-73	0.0010712746072365895
74-75	0.0010006411166495616
76-77	6.945626574391074E-4
78-79	0.0034728132871955373
80-81	0.0027547061328940872
82-83	0.0013420363211535296
84-85	0.0028488841203434577
86-87	0.001577481289776956
88-89	0.0022249549534913778
90-91	0.0032020515732785974
92-93	0.002684072642307059
94-95	0.0038848419822865334
96-97	0.003602308019938422
98-99	0.003260912815434454
100	0.0012714028305665017
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4247277.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.460301448937
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.30900422356432	48.28743708520971
2	13.884738614308	17.344899187787473
3	4.050407648156718	7.589690480848457
4	1.6997030952013346	4.246558707998665
5	0.8418753038344553	2.629189262995775
6	0.5083047406687178	1.9049320398055118
7	0.34214748007718193	1.4959444321930466
8	0.2262492251899566	1.1305275846362497
9	0.17803200289975465	1.0007939311809033
>10	0.8975905415049475	9.864149053237822
>50	0.04526107395560658	1.9235964870301456
>100	0.01593194033239647	1.6460833178782739
>500	5.278772383068067E-4	0.23616921626657075
>1k	1.1311653417278378E-4	0.12178209729744212
>5k	1.1311653417273894E-4	0.5782471156339887
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9837	0.23160721563486442	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9293	0.21879900934175	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5423	0.12768180648448405	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.3544496862342624E-5	0.0
4	0.0	0.0	0.0	2.3544496862342624E-5	0.0
5	0.0	2.3544496862342624E-5	0.0	2.3544496862342624E-5	0.0
6	0.0	2.3544496862342624E-5	0.0	4.708899372468525E-5	0.0
7	0.0	7.063349058702788E-5	0.0	4.708899372468525E-5	0.0
8	0.0	7.063349058702788E-5	0.0	4.708899372468525E-5	0.0
9	0.0	7.063349058702788E-5	0.0	4.708899372468525E-5	0.0
10-11	0.0	7.063349058702788E-5	0.0	4.708899372468525E-5	0.0
12-13	0.0	7.063349058702788E-5	0.0	4.708899372468525E-5	0.0
14-15	0.0	1.0595023588054182E-4	0.0	4.708899372468525E-5	0.0
16-17	0.0	1.4126698117405577E-4	0.0	5.886124215585657E-5	0.0
18-19	0.0	2.001282233299123E-4	0.0	8.24057390181992E-5	0.0
20-21	0.0	2.1190047176108362E-4	0.0	1.5303922960522708E-4	0.0
22-23	0.0	2.2367272019225494E-4	0.0	2.707617139169402E-4	0.0
24-25	0.0	2.472172170545976E-4	0.0	4.2380094352216724E-4	0.0
26-27	0.0	2.589894654857689E-4	0.0	7.063349058702788E-4	0.0
28-29	0.0	2.589894654857689E-4	0.0	0.0024368554252524616	0.0
30-31	0.0	2.589894654857689E-4	0.0	0.007958039939471808	0.0
32-33	0.0	2.589894654857689E-4	0.0	0.019836238606523664	0.0
34-35	0.0	2.589894654857689E-4	0.0	0.03909563703991993	0.0
36-37	0.0	2.8253396234811153E-4	0.0	0.06879701983176514	0.0
38-39	0.0	3.296229560727968E-4	0.0	0.10808101284658383	0.0
40-41	0.0	3.76711949797482E-4	0.0	0.14804779627041043	0.0
42-43	0.0	3.76711949797482E-4	0.0	0.18699039408072515	0.0
44-45	0.0	3.76711949797482E-4	0.0	0.23479749495971183	0.0
46-47	0.0	3.76711949797482E-4	0.0	0.292187206061672	0.0
48-49	0.0	3.76711949797482E-4	0.0	0.35071882526145576	0.0
50-51	0.0	4.002564466598246E-4	0.0	0.4101569075904397	0.0
52-53	0.0	4.002564466598246E-4	0.0	0.4675937076861245	0.0
54-55	0.0	4.002564466598246E-4	0.0	0.5290330722484077	0.0
56-57	0.0	4.002564466598246E-4	0.0	0.6038810277737948	0.0
58-59	0.0	4.2380094352216724E-4	0.0	0.6847916912412353	0.0
60-61	0.0	4.2380094352216724E-4	0.0	0.7655139987337769	0.0
62-63	0.0	4.2380094352216724E-4	0.0	0.8458007330343653	0.0
64-65	0.0	4.2380094352216724E-4	0.0	0.9290776184364711	0.0
66-67	0.0	4.2380094352216724E-4	0.0	1.0283176727112453	0.0
68-69	0.0	4.2380094352216724E-4	0.0	1.1345622148025665	0.0
70-71	0.0	4.2380094352216724E-4	0.0	1.2473874437669124	0.0
72-73	0.0	4.708899372468525E-4	0.0	1.3647920773709838	0.0
74-75	0.0	4.708899372468525E-4	0.0	1.4844687549222715	0.0
76-77	0.0	4.708899372468525E-4	0.0	1.612115244661462	0.0
78-79	0.0	4.708899372468525E-4	0.0	1.756560732911934	0.0
80-81	0.0	4.708899372468525E-4	0.0	1.9088112218722726	0.0
82-83	0.0	4.944344341091952E-4	0.0	2.0669360627997655	0.0
84-85	0.0	5.179789309715378E-4	0.0	2.2198928866659746	0.0
86-87	0.0	5.650679246962231E-4	0.0	2.382444093003588	0.0
88	0.0	6.121569184209083E-4	0.0	2.513893018986047	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6510	0.0	56.252663	1
GTATCAA	12500	0.0	42.12061	1
TCAACGC	15495	0.0	33.484314	4
ATCAACG	15725	0.0	33.02445	3
CAACGCA	16195	0.0	32.124073	5
TATCAAC	16265	0.0	32.072502	2
AACGCAG	16900	0.0	30.977459	6
GTGGTAT	3035	0.0	29.894085	1
TGGTATC	3060	0.0	27.337732	2
ACGCAGA	19285	0.0	26.829678	7
CGCAGAG	19530	0.0	26.324512	8
GTACATG	12520	0.0	24.06802	1
GCAGAGT	21355	0.0	23.524158	9
TACATGG	12785	0.0	22.275928	2
ACATGGG	12915	0.0	21.505869	3
GAGTACT	14455	0.0	20.719393	12-13
AGAGTAC	19745	0.0	20.594452	10-11
CAGAGTA	20665	0.0	20.280783	10-11
CATGGGG	8945	0.0	20.01744	4
AGTACTT	15135	0.0	19.617632	12-13
>>END_MODULE