FastQCFastQC Report
Wed 25 May 2016
SRR2049352_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049352_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5829479
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62850.044.203891
GTATCAA99400.034.3343281
TCAACGC108350.030.1514434
ATCAACG110050.029.7711033
CAACGCA115050.028.3559175
AACGCAG121000.027.113936
TATCAAC124350.026.7633022
GTACATG169250.024.4417481
GTGGTAT35300.023.171431
ACGCAGA142000.022.7398227
TACATGG169350.022.7326952
CGCAGAG145000.022.0748528
ACATGGG178650.020.9441263
CATGGGG126800.018.906154
GCAGAGT163750.018.2268289
TGGTATC42550.017.233652
CAGAGTA150600.016.69734610-11
GAGTACA130450.016.396291
AGAGTAC138300.016.07524710-11
GTGTTAG23200.014.79153251
Produced by FastQC (version 0.11.5)