FastQCFastQC Report
Wed 25 May 2016
SRR2049352_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049352_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5829479
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT153850.2639172385731212No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153120.2626649825824915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151660.2601604706012321No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87840.1506824194752224No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT70720.1213144433662082RNA PCR Primer, Index 46 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99250.056.7909971
GTATCAA175200.038.8798981
GTGGTAT42300.036.7856561
ATCAACG218400.030.792943
TCAACGC219100.030.716014
CAACGCA224500.029.998125
TATCAAC226100.029.8689792
TGGTATC49600.029.5622222
AACGCAG232150.029.0512686
ACGCAGA268900.024.6611277
CGCAGAG273850.023.9234058
GTACATG162600.022.9557111
GCAGAGT293650.021.8298389
GAGTACT195050.021.19894612-13
TACATGG168700.020.9770342
CAGAGTA283500.020.38841610-11
ACATGGG171350.019.9943643
AGAGTAC270100.019.97208810-11
GTACTTT209850.019.53999114-15
AGTACTT203100.018.81936612-13
Produced by FastQC (version 0.11.5)