##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049352_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5829479 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.760996308589498 31.0 31.0 34.0 30.0 34.0 2 31.832607682436116 31.0 31.0 34.0 30.0 34.0 3 31.810677077659943 33.0 31.0 34.0 30.0 34.0 4 35.58438121142559 37.0 35.0 37.0 33.0 37.0 5 35.69261575519871 37.0 35.0 37.0 33.0 37.0 6 35.76135122881479 37.0 35.0 37.0 33.0 37.0 7 35.722342253913254 37.0 35.0 37.0 33.0 37.0 8 35.734580568863876 37.0 35.0 37.0 33.0 37.0 9 37.46214215712931 39.0 37.0 39.0 34.0 39.0 10-11 37.42970023564713 39.0 37.0 39.0 34.0 39.0 12-13 37.380048028305794 39.0 37.0 39.0 34.0 39.0 14-15 38.65395698311976 40.0 38.0 41.0 34.0 41.0 16-17 38.67964101080045 40.0 38.0 41.0 34.0 41.0 18-19 38.74610595560941 40.0 38.0 41.0 34.0 41.0 20-21 38.77781599350473 40.0 38.0 41.0 34.0 41.0 22-23 38.74643883269843 40.0 38.0 41.0 34.0 41.0 24-25 38.721971294518774 40.0 38.0 41.0 34.0 41.0 26-27 38.6041913865716 40.0 38.0 41.0 34.0 41.0 28-29 38.5200903030957 40.0 38.0 41.0 34.0 41.0 30-31 38.41612560916679 40.0 38.0 41.0 34.0 41.0 32-33 38.27477129602835 40.0 38.0 41.0 34.0 41.0 34-35 38.16939361133302 40.0 38.0 41.0 34.0 41.0 36-37 38.05046685990292 40.0 38.0 41.0 33.0 41.0 38-39 37.950053169417025 40.0 37.0 41.0 33.0 41.0 40-41 37.85493171859784 40.0 37.0 41.0 33.0 41.0 42-43 37.723851050840054 40.0 37.0 41.0 33.0 41.0 44-45 37.55233846798316 40.0 37.0 41.0 33.0 41.0 46-47 37.32248808169649 40.0 37.0 41.0 32.0 41.0 48-49 37.10969719592437 40.0 37.0 41.0 31.5 41.0 50-51 36.94205854416836 40.0 36.0 41.0 31.0 41.0 52-53 36.806255756303436 40.0 36.0 41.0 31.0 41.0 54-55 36.657653882962784 39.0 36.0 41.0 31.0 41.0 56-57 36.502882590365274 39.0 35.0 41.0 31.0 41.0 58-59 36.300164560160525 39.0 35.0 41.0 30.5 41.0 60-61 36.10789060223049 39.0 35.0 41.0 30.0 41.0 62-63 35.89170438730459 38.5 35.0 40.5 30.0 41.0 64-65 35.632875425059424 38.0 35.0 40.0 29.5 41.0 66-67 35.314170700331886 37.0 35.0 40.0 29.0 41.0 68-69 34.97026561035729 37.0 34.0 40.0 29.0 41.0 70-71 34.60382257145107 36.0 34.0 39.0 28.0 41.0 72-73 34.290298241060654 36.0 34.0 39.0 28.0 41.0 74-75 33.96977800245956 35.5 34.0 39.0 28.0 40.5 76-77 33.63201702930914 35.0 34.0 37.5 28.0 39.5 78-79 33.290752998681356 35.0 34.0 37.0 27.0 39.0 80-81 32.96591985321501 35.0 33.0 36.5 27.0 39.0 82-83 32.66849172970689 35.0 33.0 36.0 26.0 38.5 84-85 32.40442241922477 35.0 33.0 36.0 26.0 37.0 86-87 32.17393303929906 35.0 33.0 35.0 26.0 37.0 88-89 31.944023642593102 35.0 33.0 35.0 26.0 36.5 90-91 31.743929088688716 35.0 33.0 35.0 25.0 36.0 92-93 31.559426751515872 35.0 33.0 35.0 25.0 36.0 94-95 31.39411137770631 35.0 33.0 35.0 24.5 36.0 96-97 31.187667800158472 35.0 33.0 35.0 24.0 35.0 98-99 31.021585462440125 35.0 33.0 35.0 23.5 35.0 100 30.894589379256704 35.0 32.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1493587300752388 1101 2 0.15055950124682127 1101 3 0.3316569196697863 1101 4 0.20126708600894716 1101 5 0.17567500059107033 1101 6 0.14859329807583777 1101 7 0.10687787627980327 1101 8 0.13330027859546334 1101 9 0.25560071194753675 1101 10-11 0.2591758467973335 1101 12-13 0.18228488472628612 1101 14-15 0.34330456272594034 1101 16-17 0.270979564573544 1101 18-19 0.32254584486315707 1101 20-21 0.3015364335327817 1101 22-23 0.27657588017227397 1101 24-25 0.28919940783173104 1101 26-27 0.37714673867950665 1101 28-29 0.36743404708114724 1101 30-31 0.36183787429469305 1101 32-33 0.40115427381861934 1101 34-35 0.3947786527956083 1101 36-37 0.36995028910043004 1101 38-39 0.40816703070908744 1101 40-41 0.40248593598548865 1101 42-43 0.40644729050978157 1101 44-45 0.44605018920704254 1101 46-47 0.38055973612217286 1101 48-49 0.47786751666013316 1101 50-51 0.42617811242200787 1101 52-53 0.47928430280260415 1101 54-55 0.4199447357557773 1101 56-57 0.3805599915151774 1101 58-59 0.3935987147153739 1101 60-61 0.3580089482742892 1101 62-63 0.3717462133459506 1101 64-65 0.3345427778447032 1101 66-67 0.33852739044441194 1101 68-69 0.34038279900048707 1101 70-71 0.32717846288937125 1101 72-73 0.3038125251321375 1101 74-75 0.3617128188841363 1101 76-77 0.2692641085479508 1101 78-79 0.30997039766040047 1101 80-81 0.3259166792734831 1101 82-83 0.2856987302496137 1101 84-85 0.31953759247215174 1101 86-87 0.2910390830056997 1101 88-89 0.39569514331848055 1101 90-91 0.42240767337811747 1101 92-93 0.4733019402637204 1101 94-95 0.4097437739583114 1101 96-97 0.46859653325119055 1101 98-99 0.5087522338349864 1101 100 0.5390284942765646 1102 1 0.24755690209010695 1102 2 0.18666675510468878 1102 3 0.3593169099533178 1102 4 0.20393912327064356 1102 5 0.1852796210534251 1102 6 0.18893617757139936 1102 7 0.12513445842317594 1102 8 0.17707028048160822 1102 9 0.14303080343149333 1102 10-11 0.1973676976172598 1102 12-13 0.21980069363796417 1102 14-15 0.3057084661073972 1102 16-17 0.2263348218910224 1102 18-19 0.2458677977726893 1102 20-21 0.24330686254982936 1102 22-23 0.24158120310275422 1102 24-25 0.2570015835697319 1102 26-27 0.3121544247578285 1102 28-29 0.3013673547593072 1102 30-31 0.24959962746937947 1102 32-33 0.2730754909921842 1102 34-35 0.25309929448449964 1102 36-37 0.3135587585109647 1102 38-39 0.28759329474053885 1102 40-41 0.2889625736846213 1102 42-43 0.3386278066287858 1102 44-45 0.3772876859618606 1102 46-47 0.3692701287240894 1102 48-49 0.33565235658289794 1102 50-51 0.37048603200660324 1102 52-53 0.38299517110709047 1102 54-55 0.3192607942649133 1102 56-57 0.3855495403536011 1102 58-59 0.3361136415554071 1102 60-61 0.37761099735905646 1102 62-63 0.398150251021832 1102 64-65 0.34650689218277364 1102 66-67 0.3777306477448832 1102 68-69 0.3639106058294388 1102 70-71 0.40779112742623624 1102 72-73 0.4239601239487314 1102 74-75 0.3933870645584605 1102 76-77 0.36194075209036214 1102 78-79 0.4313340383812587 1102 80-81 0.4423510308905847 1102 82-83 0.49581490028551656 1102 84-85 0.48748472944983234 1102 86-87 0.5095891850191734 1102 88-89 0.467328992725772 1102 90-91 0.5299414871310262 1102 92-93 0.5251612706974207 1102 94-95 0.4822172158352025 1102 96-97 0.5436853225264819 1102 98-99 0.5106443115320829 1102 100 0.5299927905328374 1103 1 0.09402677065550336 1103 2 0.1027001687253879 1103 3 0.27954605237626495 1103 4 0.14391024222076965 1103 5 0.11387309286386227 1103 6 0.07065287798036479 1103 7 0.14261337283419095 1103 8 0.122103991447986 1103 9 0.21085649448599497 1103 10-11 0.1769344036932452 1103 12-13 0.19393673175760284 1103 14-15 0.22515450733262554 1103 16-17 0.21915662274461312 1103 18-19 0.27209657583159697 1103 20-21 0.2695152506907945 1103 22-23 0.244075030084268 1103 24-25 0.24075151904905567 1103 26-27 0.25405184401408576 1103 28-29 0.2986592877887233 1103 30-31 0.27408868463943037 1103 32-33 0.2758935147520347 1103 34-35 0.3094688124206044 1103 36-37 0.28934770722503345 1103 38-39 0.35926940594038115 1103 40-41 0.30425084810934777 1103 42-43 0.3230142123758739 1103 44-45 0.3548990091162878 1103 46-47 0.36315725174269176 1103 48-49 0.29941165479750964 1103 50-51 0.250431055653884 1103 52-53 0.2426531160860108 1103 54-55 0.23727170905508643 1103 56-57 0.21532815353786106 1103 58-59 0.2980319402043179 1103 60-61 0.26244130241278896 1103 62-63 0.2437605999523953 1103 64-65 0.27223718487858406 1103 66-67 0.1878634072590657 1103 68-69 0.19829337333258223 1103 70-71 0.24315040135380173 1103 72-73 0.2730481498644082 1103 74-75 0.2270856905157288 1103 76-77 0.2231955426818928 1103 78-79 0.24546600026751975 1103 80-81 0.30790817438676044 1103 82-83 0.27960171993043303 1103 84-85 0.30993393284796866 1103 86-87 0.2670651491636633 1103 88-89 0.3282264638611032 1103 90-91 0.29851315456411953 1103 92-93 0.3055161666995758 1103 94-95 0.27020994465627624 1103 96-97 0.2676544455399217 1103 98-99 0.3318034879935077 1103 100 0.396543840433484 1104 1 0.10320402000875006 1104 2 0.12181885902706568 1104 3 0.22178478278521752 1104 4 0.19329298402873007 1104 5 0.15785209394111632 1104 6 0.1652119501661602 1104 7 0.16876271977066892 1104 8 0.17469351641874908 1104 9 0.13776059979879562 1104 10-11 0.14389305199630797 1104 12-13 0.16631302121346891 1104 14-15 0.2192356081156177 1104 16-17 0.1864337509969971 1104 18-19 0.2568121635922225 1104 20-21 0.2716998623072655 1104 22-23 0.2473576437803615 1104 24-25 0.26726324418636693 1104 26-27 0.25833392707607317 1104 28-29 0.32427886421467633 1104 30-31 0.34081313570160887 1104 32-33 0.3216926351648297 1104 34-35 0.311182060170907 1104 36-37 0.3412939507831183 1104 38-39 0.37527831204860007 1104 40-41 0.33687202984059894 1104 42-43 0.3859506878752086 1104 44-45 0.3266388568303853 1104 46-47 0.39453129202459536 1104 48-49 0.39169381435326756 1104 50-51 0.3594826236360049 1104 52-53 0.31401728900268466 1104 54-55 0.2720961422332451 1104 56-57 0.3136493072596309 1104 58-59 0.33979491554019603 1104 60-61 0.35096983675297366 1104 62-63 0.3403041093858832 1104 64-65 0.2615410587866691 1104 66-67 0.3480383856619369 1104 68-69 0.29691713067663983 1104 70-71 0.3532564531457467 1104 72-73 0.2988247554622987 1104 74-75 0.2809357322923063 1104 76-77 0.23470464311276373 1104 78-79 0.22729271829647502 1104 80-81 0.24893647648902828 1104 82-83 0.28952896338014256 1104 84-85 0.30925819408786026 1104 86-87 0.2576296296914151 1104 88-89 0.28029628852772603 1104 90-91 0.19696108538610702 1104 92-93 0.38053852033313973 1104 94-95 0.36694863342614426 1104 96-97 0.428348216436067 1104 98-99 0.37490808205123116 1104 100 0.40316526366730443 1105 1 0.172606486554848 1105 2 0.1525978833204995 1105 3 0.24547794019435898 1105 4 0.17278485580023073 1105 5 0.17059733976989833 1105 6 0.16173928017796868 1105 7 0.16003683773602972 1105 8 0.15475145861973516 1105 9 0.19023021673620377 1105 10-11 0.22108926172729326 1105 12-13 0.15543746051402252 1105 14-15 0.20673539970426447 1105 16-17 0.22662285623992062 1105 18-19 0.1589262194877108 1105 20-21 0.21709356839293292 1105 22-23 0.20565502808727842 1105 24-25 0.2072018939792315 1105 26-27 0.22903038230172257 1105 28-29 0.27222207406130394 1105 30-31 0.23483619881392315 1105 32-33 0.3442005714295746 1105 34-35 0.3010272714319058 1105 36-37 0.3072942387552615 1105 38-39 0.35339966542967716 1105 40-41 0.3504587605485554 1105 42-43 0.3242352804297539 1105 44-45 0.3381827387463332 1105 46-47 0.35944078344495267 1105 48-49 0.3563436563675211 1105 50-51 0.28643972424210773 1105 52-53 0.37957148787061357 1105 54-55 0.34410861478821886 1105 56-57 0.3018048159820097 1105 58-59 0.30648537975771006 1105 60-61 0.29272411725720104 1105 62-63 0.27258192213672316 1105 64-65 0.315981385691245 1105 66-67 0.3835236299920126 1105 68-69 0.3507334295766711 1105 70-71 0.3433193200839355 1105 72-73 0.37212911999241527 1105 74-75 0.3491811016304993 1105 76-77 0.32434847134935296 1105 78-79 0.30827512954417813 1105 80-81 0.27702282603944894 1105 82-83 0.35668993098245494 1105 84-85 0.370991056398708 1105 86-87 0.3810772301457632 1105 88-89 0.42229913320690926 1105 90-91 0.43201594999082715 1105 92-93 0.49000927980214826 1105 94-95 0.4304371344603588 1105 96-97 0.4875215553365777 1105 98-99 0.5543675171958817 1105 100 0.594712663593473 1106 1 0.14327953718919062 1106 2 0.08922212966902165 1106 3 0.20668156948489624 1106 4 0.16549550882975694 1106 5 0.11570079787016851 1106 6 0.1528856680866042 1106 7 0.16609491522654452 1106 8 0.14490971815907727 1106 9 0.16477205364409286 1106 10-11 0.13280997792127636 1106 12-13 0.1579422961252135 1106 14-15 0.2430493305691428 1106 16-17 0.2032382632088101 1106 18-19 0.22324899412735277 1106 20-21 0.2168781295205875 1106 22-23 0.19339051165550813 1106 24-25 0.2164386772775373 1106 26-27 0.2621722452959858 1106 28-29 0.2681583444351503 1106 30-31 0.2750915389923634 1106 32-33 0.18476987246319965 1106 34-35 0.20019745172396597 1106 36-37 0.2296006873011649 1106 38-39 0.2734314344548068 1106 40-41 0.25427999540652735 1106 42-43 0.28804670768706586 1106 44-45 0.2530188450396764 1106 46-47 0.25290349471976015 1106 48-49 0.2592859107092309 1106 50-51 0.26910479332254766 1106 52-53 0.2030287621125595 1106 54-55 0.20889611612776804 1106 56-57 0.20950981933200552 1106 58-59 0.2824664209818266 1106 60-61 0.24872537478110956 1106 62-63 0.25673458719416686 1106 64-65 0.1393797005730093 1106 66-67 0.17966776410445817 1106 68-69 0.2393183945908035 1106 70-71 0.2845351833447509 1106 72-73 0.3514896080688814 1106 74-75 0.28892299381359265 1106 76-77 0.2670705840308898 1106 78-79 0.2950434133123139 1106 80-81 0.2970391604063707 1106 82-83 0.3075384558415024 1106 84-85 0.2869349936869483 1106 86-87 0.27758170018564954 1106 88-89 0.2993424188914453 1106 90-91 0.27887479668059 1106 92-93 0.2944674980919153 1106 94-95 0.19733797531312902 1106 96-97 0.3112759919928827 1106 98-99 0.3013825977876792 1106 100 0.28278356012348027 1107 1 0.14381626874695996 1107 2 0.10797867247755377 1107 3 0.2260699880092254 1107 4 0.17402386006899917 1107 5 0.11215529394319645 1107 6 0.15069877101515772 1107 7 0.12092908331522523 1107 8 0.12312041294492815 1107 9 0.13180548792136904 1107 10-11 0.12020380380734963 1107 12-13 0.10833291568202696 1107 14-15 0.18042313965088397 1107 16-17 0.13286600211838362 1107 18-19 0.15196855135488363 1107 20-21 0.17296984753483713 1107 22-23 0.1850293751127765 1107 24-25 0.16050724434174413 1107 26-27 0.2238961421968284 1107 28-29 0.22042847852200964 1107 30-31 0.19029036944356648 1107 32-33 0.19734586131152554 1107 34-35 0.19093923093102205 1107 36-37 0.20341807956286573 1107 38-39 0.192000036480934 1107 40-41 0.19366115289628993 1107 42-43 0.2273774496947496 1107 44-45 0.1933046817414663 1107 46-47 0.34275796269771064 1107 48-49 0.2958428031872913 1107 50-51 0.2540638439247047 1107 52-53 0.24701922032978985 1107 54-55 0.20294494256807383 1107 56-57 0.1560318477969176 1107 58-59 0.22788469435952408 1107 60-61 0.16834922456808243 1107 62-63 0.1950885606208388 1107 64-65 0.18235734238632517 1107 66-67 0.24662293889088716 1107 68-69 0.1954047082561985 1107 70-71 0.20422699862473337 1107 72-73 0.2730720541412026 1107 74-75 0.22682544362187684 1107 76-77 0.20501056927029992 1107 78-79 0.23795615272465653 1107 80-81 0.2542973076348929 1107 82-83 0.28288935876947363 1107 84-85 0.2838367827963282 1107 86-87 0.21132432663893752 1107 88-89 0.2384704444637471 1107 90-91 0.23665437256034494 1107 92-93 0.28144020531416913 1107 94-95 0.31625833077742627 1107 96-97 0.2785436774891572 1107 98-99 0.3289651264630571 1107 100 0.4023298534243338 1108 1 0.08203397685769431 1108 2 0.07647992814555948 1108 3 0.17055262789684988 1108 4 0.14282576620975362 1108 5 0.08559995520367636 1108 6 0.09546175181745298 1108 7 0.0540682681181579 1108 8 0.05070404630021841 1108 9 0.19358132215988633 1108 10-11 0.14947838040605177 1108 12-13 0.12947527656463365 1108 14-15 0.20196565305236192 1108 16-17 0.11152134669936231 1108 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20-21 -0.4198219514030157 2215 22-23 -0.3681470167697256 2215 24-25 -0.35340001137168997 2215 26-27 -0.43795594263240645 2215 28-29 -0.39836700594212004 2215 30-31 -0.3604650390593491 2215 32-33 -0.3275086302648518 2215 34-35 -0.38446737804795106 2215 36-37 -0.39932788430625976 2215 38-39 -0.38202153267256733 2215 40-41 -0.43332977354080526 2215 42-43 -0.41580091110405704 2215 44-45 -0.43042922605111755 2215 46-47 -0.451001404382815 2215 48-49 -0.4363005227711838 2215 50-51 -0.4641344361120261 2215 52-53 -0.40152010368552027 2215 54-55 -0.27728723172781855 2215 56-57 -0.38137652365397656 2215 58-59 -0.42739847950296195 2215 60-61 -0.4211839599565437 2215 62-63 -0.40968941253295554 2215 64-65 -0.3733230292450429 2215 66-67 -0.4123448431285013 2215 68-69 -0.4688994938379025 2215 70-71 -0.45028933184953246 2215 72-73 -0.42716991467717236 2215 74-75 -0.3823272366236452 2215 76-77 -0.35707415787412344 2215 78-79 -0.4328046225889892 2215 80-81 -0.4532120488709239 2215 82-83 -0.3664703051415188 2215 84-85 -0.42551461546980107 2215 86-87 -0.41132467154509555 2215 88-89 -0.47694100035029763 2215 90-91 -0.4853055442646905 2215 92-93 -0.4047640810212165 2215 94-95 -0.4471737957241011 2215 96-97 -0.49141825818007234 2215 98-99 -0.451639723226787 2215 100 -0.43623193967060914 2216 1 -0.44501278023607327 2216 2 -0.37453875703766926 2216 3 -0.514031570788859 2216 4 -0.3111315184703116 2216 5 -0.515307943065892 2216 6 -0.3603032713368606 2216 7 -0.6767948665678105 2216 8 -0.39538150762114554 2216 9 -0.6327778019169799 2216 10-11 -0.48256046842017497 2216 12-13 -0.48890720120766673 2216 14-15 -0.7517181280488145 2216 16-17 -0.5323546502422474 2216 18-19 -0.4682865941615688 2216 20-21 -0.564315338078309 2216 22-23 -0.5676078676561929 2216 24-25 -0.5272963635401737 2216 26-27 -0.6494315404064679 2216 28-29 -0.6801859909987442 2216 30-31 -0.8081142691298524 2216 32-33 -0.7553687732386507 2216 34-35 -0.717866385540475 2216 36-37 -0.7848022395142422 2216 38-39 -0.7341642974422768 2216 40-41 -0.7341051399099214 2216 42-43 -0.8156365536562689 2216 44-45 -0.957348321747979 2216 46-47 -0.9259709930527507 2216 48-49 -0.9990153184262596 2216 50-51 -0.8981862952420059 2216 52-53 -0.8200865234261059 2216 54-55 -0.7703356000894246 2216 56-57 -0.883196352467273 2216 58-59 -0.91208852582416 2216 60-61 -0.7788269228329483 2216 62-63 -0.7031899786837101 2216 64-65 -0.7880892912882302 2216 66-67 -0.6909171669261056 2216 68-69 -0.7148898146009373 2216 70-71 -0.8256601335377809 2216 72-73 -0.7292963810365407 2216 74-75 -0.736367502040089 2216 76-77 -0.7311213671799663 2216 78-79 -0.8726162866922635 2216 80-81 -1.0221280030605158 2216 82-83 -0.9276802452914978 2216 84-85 -1.030685479708854 2216 86-87 -0.9199695175931488 2216 88-89 -1.2318628438759696 2216 90-91 -1.0240227924337368 2216 92-93 -1.097595379539836 2216 94-95 -1.0237044299400928 2216 96-97 -1.117775727321746 2216 98-99 -1.254176019347348 2216 100 -1.2680372111361358 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 282.0 3 34.0 4 41.0 5 126.0 6 259.0 7 750.0 8 1725.0 9 3225.0 10 4871.0 11 6780.0 12 9176.0 13 12449.0 14 14711.0 15 19025.0 16 24221.0 17 33685.0 18 33442.0 19 20230.0 20 16454.0 21 17420.0 22 18947.0 23 21836.0 24 27192.0 25 33055.0 26 39076.0 27 44774.0 28 56701.0 29 74503.0 30 97343.0 31 125127.0 32 161291.0 33 210729.0 34 286480.0 35 415251.0 36 674191.0 37 1200038.0 38 1560920.0 39 561054.0 40 2065.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.93558320108507 20.617325940243923 14.865727978605983 23.581362880065015 2 15.41033083745563 20.451690451239298 39.868863066493596 24.269115644811485 3 17.946389377163893 25.153002523896216 30.990745485145414 25.909862613794477 4 12.913081724669592 17.456714805927668 37.36622296755318 32.26398050184957 5 12.897035910070178 37.673229460128425 35.51221301251793 13.917521617283466 6 30.85790871841355 37.868828049441134 16.990577887076682 14.282685345068632 7 26.633306338353734 33.02895507471594 21.986784753834776 18.35095383309555 8 24.929350013260272 36.529173804285215 19.937088318230114 18.604387864224396 9 24.733857005059974 16.67509223379997 21.28881500387942 37.30223575726064 10-11 24.0695609333184 26.95319770428884 28.282587517683826 20.694653844708935 12-13 24.63282330778486 24.326754777746142 28.700079269423885 22.340342645045112 14-15 21.974080845222275 26.261261203307846 26.24429568884663 25.520362262623248 16-17 21.156118754351805 29.708675509423742 27.08213718584457 22.053068550379887 18-19 21.036713839276334 27.972755705807252 29.84609684144076 21.14443361347565 20-21 21.877593233762877 27.118718354239025 29.764497599997668 21.239190812000434 22-23 21.709058390981422 27.112045518990634 29.748876357561283 21.430019732466658 24-25 21.65455793813248 27.27938934440466 29.682282573594783 21.383770143868077 26-27 21.344352852418588 27.357674122914005 29.81417362870132 21.483799395966084 28-29 21.33366555392781 27.13374823740106 30.086011397282082 21.44657481138905 30-31 21.616393301733595 27.52905770215981 29.586754629938167 21.26779436616843 32-33 21.64890889681681 27.140071296398112 29.84156184433328 21.369457962451797 34-35 21.636104171284007 26.90333573720863 29.874794921533255 21.58576516997411 36-37 21.6588439217216 27.20157242276771 29.522921916388533 21.616661739122158 38-39 21.486316879760892 26.98268888723636 29.842548975561815 21.68844525744093 40-41 21.38006725997966 26.774057227677407 30.011401552062328 21.834473960280608 42-43 21.40697173952461 26.829859906142072 29.93081335817502 21.832354996158298 44-45 21.27358846192674 27.02975084079321 29.763835123477445 21.932825573802607 46-47 21.52013754246203 27.00729783012099 29.3641062720512 22.108458355365773 48-49 21.42263051804262 27.080109928348957 29.121689097233887 22.375570456374536 50-51 21.239297457601534 27.08904570169155 29.088657672345413 22.582999168361507 52-53 21.209351833665128 27.15563831548286 29.23670263635732 22.39830721449469 54-55 21.202246863345835 27.300350289735242 28.918235994414577 22.579166852504347 56-57 21.266033456666847 27.188383605934764 28.69428013032445 22.851302807073942 58-59 21.072019901630885 27.049619588189117 28.98489777461835 22.89346273556164 60-61 21.29687056536772 27.426731565631634 28.477538658521617 22.798859210479026 62-63 20.924601944187486 27.575357957535278 28.467220837723772 23.03281926055346 64-65 20.94441614466948 27.456999185258102 28.430195043154562 23.168389626917858 66-67 20.99080735325967 27.517848144423407 28.27483161824463 23.216512884072298 68-69 21.255601788729795 27.64578374265017 27.972687698948846 23.12592676967119 70-71 21.297303315198988 27.471191984703886 27.939546310855185 23.291958389241945 72-73 21.144679622154616 27.573447897016884 27.9215870734343 23.360285407394198 74-75 21.36991513477457 27.452570534681886 27.80749438258818 23.370019947955367 76-77 21.49208036863931 27.409323162884657 27.60648625133332 23.492110217142717 78-79 21.40918280435012 27.503338757116037 27.679973375717175 23.407505062816668 80-81 21.340661014655076 27.44888718409431 27.68721723716576 23.523234564084845 82-83 21.280506591077362 27.357225345473186 27.75575720023138 23.606510863218073 84-85 21.48513416264889 27.147781768152463 27.636253378404458 23.730830690794186 86-87 21.515782279643172 27.25606092991612 27.588909020606973 23.639247769833734 88-89 21.38913920263165 27.07280793350752 27.69679677892102 23.841256084939815 90-91 21.27342978909405 26.928178138575536 27.697077756017034 24.10131431631338 92-93 21.3217279843879 26.92453935692538 27.726870879614076 24.02686177907265 94-95 21.27462165545152 26.654951024090863 27.937201456758498 24.133225863699124 96-97 21.360610922946982 26.66117256192169 27.88056875306266 24.097647762068668 98-99 21.309400530270093 26.716445140393084 27.793471026977794 24.180683302359025 100 21.30861399024838 26.688989189089973 27.83104713032532 24.171349690336328 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 687.0 1 542.5 2 661.5 3 986.5 4 694.5 5 442.0 6 1160.0 7 3224.0 8 5475.5 9 6229.0 10 4786.0 11 3024.5 12 2771.0 13 3120.0 14 3493.5 15 3904.0 16 4511.5 17 5159.5 18 5856.5 19 6673.5 20 7910.5 21 9723.5 22 12248.0 23 16020.5 24 21113.0 25 27632.0 26 36159.0 27 46553.0 28 59156.0 29 73192.0 30 85968.5 31 97522.0 32 108585.0 33 119857.0 34 130590.0 35 140629.0 36 151932.0 37 164968.5 38 177553.5 39 187071.0 40 196302.0 41 204649.5 42 211124.0 43 217853.5 44 221115.0 45 221966.5 46 222549.5 47 217839.5 48 210262.5 49 205551.5 50 200893.5 51 192984.0 52 182073.5 53 171752.5 54 162743.5 55 152703.0 56 139659.0 57 124030.0 58 109928.5 59 98112.5 60 85227.5 61 71451.5 62 59649.5 63 49391.0 64 39868.0 65 31262.0 66 24252.5 67 18557.5 68 13833.0 69 10348.0 70 7636.0 71 5510.5 72 3883.5 73 2536.5 74 1698.0 75 1130.5 76 696.5 77 407.5 78 242.5 79 150.0 80 82.5 81 47.5 82 30.0 83 18.5 84 11.5 85 6.5 86 3.5 87 2.5 88 4.0 89 2.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03525186384580852 2 0.0 3 0.0 4 5.14625749573847E-5 5 0.0 6 1.7154191652461566E-5 7 0.0 8 6.347050911410779E-4 9 0.0 10-11 0.0 12-13 0.0431599461975933 14-15 1.1150224574100018E-4 16-17 0.0 18-19 2.573128747869235E-5 20-21 9.434805408853862E-5 22-23 0.0 24-25 1.1150224574100018E-4 26-27 8.577095826230783E-5 28-29 3.2592964139676976E-4 30-31 8.577095826230783E-6 32-33 2.058502998295388E-4 34-35 5.14625749573847E-5 36-37 6.003967078361549E-5 38-39 2.658899706131543E-4 40-41 1.6296482069838488E-4 42-43 5.14625749573847E-4 44-45 2.3158158730823115E-4 46-47 1.1150224574100018E-4 48-49 7.805157201870014E-4 50-51 3.94546408006616E-4 52-53 5.832425161836932E-4 54-55 3.2592964139676976E-4 56-57 4.803173662689239E-4 58-59 1.2007934156723097E-4 60-61 1.2865643739346175E-4 62-63 8.834408701017706E-4 64-65 4.3743188713777E-4 66-67 0.0015696085362002334 68-69 0.0015610314403740027 70-71 4.2885479131153913E-4 72-73 9.8636602001654E-4 74-75 0.0010206744033214633 76-77 7.033218577509242E-4 78-79 0.003670997013626775 80-81 0.002881904197613543 82-83 0.00142379790715431 84-85 0.0029848293475283126 86-87 0.0015953398236789257 88-89 0.0021957365315150804 90-91 0.0029934064433545433 92-93 0.002667476801957774 94-95 0.004039812134154699 96-97 0.003439415426318544 98-99 0.0031821025515316203 100 0.001543877248721541 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5829479.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.70460514095701 #Duplication Level Percentage of deduplicated Percentage of total 1 75.05146907620818 42.557639192151306 2 14.361601432068156 16.287378767944553 3 4.56036369673776 7.757808681680091 4 2.03930306292318 4.625514997832125 5 1.095481679021373 3.105942802402978 6 0.6321540133202084 2.1507626228156216 7 0.4324614076479679 1.7165787351566326 8 0.30542245848294536 1.38550879275646 9 0.22974354097641214 1.1724765097277454 >10 1.1877697228418846 12.1094306068039 >50 0.07520203778340907 2.9063805722038283 >100 0.028058903458953597 2.616556251487581 >500 3.936486969294261E-4 0.15046465186694544 >1k 4.2391987687096855E-4 0.39783236256787463 >5k 6.055998233731402E-5 0.2722498893446957 >10k+ 9.083997350597104E-5 0.7874745632578064 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 15385 0.2639172385731212 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15312 0.2626649825824915 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15166 0.2601604706012321 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8784 0.1506824194752224 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 7072 0.1213144433662082 RNA PCR Primer, Index 46 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.7154191652461566E-5 0.0 3 0.0 0.0 0.0 3.430838330492313E-5 0.0 4 0.0 0.0 0.0 3.430838330492313E-5 0.0 5 0.0 0.0 0.0 3.430838330492313E-5 0.0 6 0.0 0.0 0.0 3.430838330492313E-5 0.0 7 0.0 0.0 0.0 3.430838330492313E-5 0.0 8 0.0 0.0 0.0 1.029251499147694E-4 0.0 9 0.0 0.0 0.0 1.7154191652461566E-4 0.0 10-11 0.0 0.0 0.0 2.4015868313446192E-4 0.0 12-13 0.0 1.7154191652461566E-5 0.0 3.516609288754621E-4 0.0 14-15 0.0 9.434805408853862E-5 0.0 4.202776954853084E-4 0.0 16-17 0.0 1.7154191652461569E-4 0.0 4.888944620951546E-4 0.0 18-19 0.0 2.058502998295388E-4 0.0 6.261279953148472E-4 0.0 20-21 0.0 2.058502998295388E-4 0.0 9.692118283640785E-4 0.0 22-23 0.0 2.144273956557696E-4 0.0 0.0021700052440363883 0.0 24-25 0.0 2.3158158730823115E-4 0.0 0.004854636237646624 0.0 26-27 0.0 2.4015868313446192E-4 0.0 0.01018101274573594 0.0 28-29 0.0 2.487357789606927E-4 0.0 0.03433411459240183 0.0 30-31 0.0 2.573128747869235E-4 0.0 0.10205028614049386 0.0 32-33 0.0 2.573128747869235E-4 0.0 0.24501331937210855 0.0 34-35 0.0 2.573128747869235E-4 0.0 0.4626056633877573 0.0 36-37 0.0 2.7446706643938505E-4 0.0 0.7950796288999411 0.0 38-39 0.0 3.001983539180774E-4 0.0 1.2176988715458106 0.0 40-41 0.0 3.430838330492313E-4 0.0 1.622623565502166 0.0 42-43 0.0 3.773922163541545E-4 0.0 2.015145778893791 0.0 44-45 0.0 3.773922163541545E-4 0.0 2.4787034999182604 0.0 46-47 0.0 3.773922163541545E-4 0.0 3.0207244935610884 0.0 48-49 0.0 3.859693121803853E-4 0.0 3.55559218928484 0.0 50-51 0.0 4.117005996590776E-4 0.0 4.059307872967722 0.0 52-53 0.0 4.288547913115392E-4 0.0 4.531768619459818 0.0 54-55 0.0 4.288547913115392E-4 0.0 5.029626146693383 0.0 56-57 0.0 4.288547913115392E-4 0.0 5.592961223464395 0.0 58-59 0.0 4.288547913115392E-4 0.0 6.17517448814894 0.0 60-61 0.0 4.288547913115392E-4 0.0 6.747060929458705 0.0 62-63 0.0 4.288547913115392E-4 0.0 7.279750042842593 0.0 64-65 0.0 4.288547913115392E-4 0.0 7.8229203673261365 0.0 66-67 0.0 4.288547913115392E-4 0.0 8.415872155985124 0.0 68-69 0.0 4.288547913115392E-4 0.0 9.041759992616836 0.0 70-71 0.0 4.288547913115392E-4 0.0 9.674389426567966 0.0 72-73 0.0 4.8031736626892384E-4 0.0 10.291494317073617 0.0 74-75 0.0 4.974715579213854E-4 0.0 10.883691321299896 0.0 76-77 0.0 4.974715579213854E-4 0.0 11.500916291147117 0.0 78-79 8.577095826230783E-6 5.060486537476162E-4 0.0 12.160992431742185 0.0 80-81 1.7154191652461566E-5 5.14625749573847E-4 0.0 12.831532629245254 0.0 82-83 1.7154191652461566E-5 5.232028454000778E-4 0.0 13.483657802009407 0.0 84-85 1.7154191652461566E-5 5.317799412263086E-4 0.0 14.116681782368545 0.0 86-87 1.7154191652461566E-5 5.489341328787701E-4 0.0 14.753634072616094 0.0 88 1.7154191652461566E-5 5.832425161836932E-4 0.0 15.249664678438673 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9925 0.0 56.790997 1 GTATCAA 17520 0.0 38.879898 1 GTGGTAT 4230 0.0 36.785656 1 ATCAACG 21840 0.0 30.79294 3 TCAACGC 21910 0.0 30.71601 4 CAACGCA 22450 0.0 29.99812 5 TATCAAC 22610 0.0 29.868979 2 TGGTATC 4960 0.0 29.562222 2 AACGCAG 23215 0.0 29.051268 6 ACGCAGA 26890 0.0 24.661127 7 CGCAGAG 27385 0.0 23.923405 8 GTACATG 16260 0.0 22.955711 1 GCAGAGT 29365 0.0 21.829838 9 GAGTACT 19505 0.0 21.198946 12-13 TACATGG 16870 0.0 20.977034 2 CAGAGTA 28350 0.0 20.388416 10-11 ACATGGG 17135 0.0 19.994364 3 AGAGTAC 27010 0.0 19.972088 10-11 GTACTTT 20985 0.0 19.539991 14-15 AGTACTT 20310 0.0 18.819366 12-13 >>END_MODULE