FastQCFastQC Report
Wed 25 May 2016
SRR2049351_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049351_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5194478
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51350.047.234461
GTATCAA87900.034.4386941
TCAACGC92800.031.4546574
ATCAACG95900.030.3888593
CAACGCA100000.029.0961915
AACGCAG105400.027.5147116
TATCAAC109400.027.1114862
GTACATG143150.025.6125581
TACATGG145050.024.207182
ACGCAGA122850.023.1470937
CGCAGAG123750.022.5609538
TAGACCG2304.101821E-922.4807265
ACATGGG147100.022.4638393
CATGGGG109900.019.2466954
GCAGAGT144050.018.5986759
GTGGTAT30350.018.5854131
ACCGCGT3157.772542E-917.905528
GAGTACA107050.017.1687931
AGAGTAC122300.016.8524710-11
CAGAGTA137000.016.79393810-11
Produced by FastQC (version 0.11.5)