##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049351_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5194478 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.566714307000627 31.0 31.0 34.0 27.0 34.0 2 30.806315283268116 31.0 31.0 34.0 27.0 34.0 3 30.972907383571552 31.0 31.0 34.0 27.0 34.0 4 34.587385873999274 37.0 35.0 37.0 32.0 37.0 5 34.613282605104885 37.0 35.0 37.0 32.0 37.0 6 34.60732242970324 37.0 35.0 37.0 32.0 37.0 7 34.51644380821326 37.0 35.0 37.0 31.0 37.0 8 34.47701905754534 37.0 35.0 37.0 31.0 37.0 9 36.058046063531314 39.0 37.0 39.0 31.0 39.0 10-11 36.01746595134294 39.0 37.0 39.0 31.0 39.0 12-13 35.94821587462686 39.0 37.0 39.0 30.0 39.0 14-15 37.13291499165075 40.0 37.0 41.0 31.0 41.0 16-17 37.094354331657584 40.0 37.0 41.0 31.0 41.0 18-19 37.119178192688466 40.0 37.0 41.0 31.0 41.0 20-21 37.18121503257883 40.0 37.0 41.0 31.0 41.0 22-23 37.13266578470445 40.0 37.0 41.0 31.0 41.0 24-25 37.131865415543196 40.0 37.0 41.0 31.0 41.0 26-27 37.10779379564222 40.0 37.0 41.0 31.0 41.0 28-29 37.047670237509905 40.0 37.0 41.0 31.0 41.0 30-31 36.987103323952866 40.0 37.0 41.0 30.5 41.0 32-33 36.92302075396219 40.0 37.0 41.0 30.0 41.0 34-35 36.82516308664701 40.0 37.0 41.0 30.0 41.0 36-37 36.752497844826756 40.0 37.0 41.0 30.0 41.0 38-39 36.655379424073026 40.0 37.0 41.0 30.0 41.0 40-41 36.54931111461056 40.0 36.5 41.0 30.0 41.0 42-43 36.41597490642948 40.0 36.0 41.0 30.0 41.0 44-45 36.29672548425462 40.0 36.0 41.0 30.0 41.0 46-47 36.173910063725366 40.0 36.0 41.0 29.0 41.0 48-49 36.04747926548154 40.0 35.5 41.0 29.0 41.0 50-51 35.908356527835906 39.0 35.0 41.0 28.5 41.0 52-53 35.76296752050928 39.0 35.0 41.0 28.0 41.0 54-55 35.624081380265736 39.0 35.0 41.0 28.0 41.0 56-57 35.49171081290555 39.0 35.0 41.0 28.0 41.0 58-59 35.28566604767602 39.0 35.0 40.5 27.5 41.0 60-61 35.06663094925034 38.5 34.0 40.0 27.0 41.0 62-63 34.85436717606659 38.0 34.0 40.0 26.5 41.0 64-65 34.608642389090875 38.0 34.0 40.0 26.0 41.0 66-67 34.34489298058438 37.0 34.0 40.0 26.0 41.0 68-69 34.0584260824668 37.0 34.0 40.0 26.0 41.0 70-71 33.772397534458705 36.0 33.5 39.0 26.0 41.0 72-73 33.43311204706228 36.0 33.0 39.0 25.0 41.0 74-75 33.08909393783168 35.5 33.0 39.0 25.0 40.0 76-77 32.75858536699934 35.0 33.0 37.5 24.0 40.0 78-79 32.44717024501789 35.0 33.0 37.0 24.0 39.0 80-81 32.09787018060332 35.0 32.5 37.0 22.5 39.0 82-83 31.791247744239172 35.0 32.0 36.0 22.0 39.0 84-85 31.505002138809715 35.0 32.0 36.0 21.5 37.0 86-87 31.194011024784395 35.0 32.0 35.5 20.0 37.0 88-89 30.95140589680041 35.0 32.0 35.0 19.5 36.5 90-91 30.751525754849666 35.0 32.0 35.0 18.5 36.0 92-93 30.520278746006817 35.0 32.0 35.0 16.0 36.0 94-95 30.323236425296248 35.0 32.0 35.0 8.5 36.0 96-97 30.075365801915034 34.5 31.0 35.0 2.0 35.5 98-99 29.87161395235479 34.0 31.0 35.0 2.0 35.0 100 29.74556577196015 34.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07765705491330976 1101 2 0.10659371146073227 1101 3 0.11316684454309467 1101 4 0.22909057447624548 1101 5 0.1612770609157721 1101 6 0.23056117484809135 1101 7 0.21977939608326835 1101 8 0.1973758227072011 1101 9 0.3493491344681132 1101 10-11 0.3836905052627557 1101 12-13 0.36921482621126955 1101 14-15 0.46926926597290475 1101 16-17 0.466111328004402 1101 18-19 0.4066171791569957 1101 20-21 0.39467180897091225 1101 22-23 0.44370383520927703 1101 24-25 0.4442121634669647 1101 26-27 0.3741496831567872 1101 28-29 0.3871724368414675 1101 30-31 0.3320359454718371 1101 32-33 0.3703538539532545 1101 34-35 0.3572258506122523 1101 36-37 0.3847819976353719 1101 38-39 0.4259802220694979 1101 40-41 0.45886069778079985 1101 42-43 0.4267207102365447 1101 44-45 0.40547158451304455 1101 46-47 0.38685882672287875 1101 48-49 0.284673733542256 1101 50-51 0.3423502325171839 1101 52-53 0.35315393622455815 1101 54-55 0.38584747360305727 1101 56-57 0.3639491925215097 1101 58-59 0.3924196124353756 1101 60-61 0.42554956603500926 1101 62-63 0.4163689170175502 1101 64-65 0.32678226978028135 1101 66-67 0.34522922667529343 1101 68-69 0.31953845533352876 1101 70-71 0.3372262185593371 1101 72-73 0.4343811989001978 1101 74-75 0.3993433544664384 1101 76-77 0.4327395188094201 1101 78-79 0.37225082964122436 1101 80-81 0.40628606871146644 1101 82-83 0.40960025599734706 1101 84-85 0.3962238850089044 1101 86-87 0.4184273293722356 1101 88-89 0.49301431490739134 1101 90-91 0.5394043503558414 1101 92-93 0.47074369209604683 1101 94-95 0.4433552455283447 1101 96-97 0.43044894597284156 1101 98-99 0.5156889737973387 1101 100 0.5768765076018418 1102 1 0.1621333502453659 1102 2 0.20291220935612841 1102 3 0.28178198462677884 1102 4 0.35060380390129353 1102 5 0.2803709337033595 1102 6 0.4290285267446379 1102 7 0.4487661058354462 1102 8 0.414515868987408 1102 9 0.5718906548041076 1102 10-11 0.4517590810953678 1102 12-13 0.5060597267664662 1102 14-15 0.6129710892320546 1102 16-17 0.5808868010365771 1102 18-19 0.6328974556441125 1102 20-21 0.5570079885355241 1102 22-23 0.5711974187927211 1102 24-25 0.5800189162188758 1102 26-27 0.5881142659442986 1102 28-29 0.6030587704875785 1102 30-31 0.5915512265717027 1102 32-33 0.6920809410536961 1102 34-35 0.677973914106083 1102 36-37 0.661202947160362 1102 38-39 0.6599972582795601 1102 40-41 0.7126920349433092 1102 42-43 0.6249905725608897 1102 44-45 0.5945054014603457 1102 46-47 0.5893157836728804 1102 48-49 0.6154193881454262 1102 50-51 0.6408841634750075 1102 52-53 0.6125471600014123 1102 54-55 0.6406468622526873 1102 56-57 0.6222203090474778 1102 58-59 0.7019570179263113 1102 60-61 0.5534872330724454 1102 62-63 0.5570277057195767 1102 64-65 0.6481309002555946 1102 66-67 0.7057621782920975 1102 68-69 0.6797387089533373 1102 70-71 0.7058722075419226 1102 72-73 0.7356745964637526 1102 74-75 0.7013334258564186 1102 76-77 0.7536962670403611 1102 78-79 0.7619436772090609 1102 80-81 0.6878148100883976 1102 82-83 0.730177063493187 1102 84-85 0.7604181188041004 1102 86-87 0.7757025995188584 1102 88-89 0.6530508703378715 1102 90-91 0.688619899573478 1102 92-93 0.6317517991111856 1102 94-95 0.6271095513775045 1102 96-97 0.5861788286904073 1102 98-99 0.7306130722948865 1102 100 0.6942177923332338 1103 1 0.03967385541515611 1103 2 0.12467631530090983 1103 3 0.19453342663678796 1103 4 0.19409710463777685 1103 5 0.18798050840245395 1103 6 0.23817028494796233 1103 7 0.23484474701670166 1103 8 0.19627848576099893 1103 9 0.33610275832558756 1103 10-11 0.31099996583999 1103 12-13 0.3899811584874371 1103 14-15 0.3839302522752419 1103 16-17 0.38625603317060353 1103 18-19 0.33952753577185035 1103 20-21 0.3670359182771108 1103 22-23 0.3471672579155438 1103 24-25 0.3497199753406903 1103 26-27 0.3438271828989201 1103 28-29 0.3368413070965772 1103 30-31 0.3942225359357181 1103 32-33 0.3795259336664074 1103 34-35 0.36102182159529406 1103 36-37 0.3560271106913433 1103 38-39 0.3797351166400489 1103 40-41 0.3225149128559224 1103 42-43 0.34967265949425297 1103 44-45 0.28489976397261074 1103 46-47 0.30349276716412277 1103 48-49 0.27388330723935894 1103 50-51 0.17707739629776142 1103 52-53 0.25862141230517466 1103 54-55 0.2191414117468682 1103 56-57 0.1419644091486134 1103 58-59 0.19763860986178372 1103 60-61 0.1629483931040454 1103 62-63 0.2073246102503532 1103 64-65 0.24305069675519064 1103 66-67 0.2819424181371275 1103 68-69 0.2980121746831088 1103 70-71 0.18906937618351805 1103 72-73 0.2508822617144517 1103 74-75 0.26049715711656063 1103 76-77 0.3597615105956251 1103 78-79 0.2180535836132549 1103 80-81 0.19632849518669104 1103 82-83 0.21265026394078745 1103 84-85 0.2674331636107006 1103 86-87 0.2763654117604979 1103 88-89 0.21665674684658853 1103 90-91 0.26276588078124874 1103 92-93 0.2437180022972072 1103 94-95 0.28696913023366477 1103 96-97 0.2652522054303219 1103 98-99 0.3316158955767108 1103 100 0.29717944093257387 1104 1 0.01697541510992906 1104 2 0.10342566284962729 1104 3 0.16658931246222153 1104 4 0.2004067088247794 1104 5 0.23499267600713125 1104 6 0.24866285016317136 1104 7 0.25722864585106464 1104 8 0.24375222114769457 1104 9 0.34658705751589736 1104 10-11 0.36101523155399917 1104 12-13 0.3313036112970451 1104 14-15 0.4496333439335629 1104 16-17 0.4802400074308366 1104 18-19 0.402951807714075 1104 20-21 0.4060073822450647 1104 22-23 0.429273394924806 1104 24-25 0.4575599539412991 1104 26-27 0.41784292400548395 1104 28-29 0.4081611867054491 1104 30-31 0.36585107948727824 1104 32-33 0.4256274288221107 1104 34-35 0.5013239457666572 1104 36-37 0.39637778334152074 1104 38-39 0.4948617243478495 1104 40-41 0.49195984495845835 1104 42-43 0.44576219534589967 1104 44-45 0.3948892935142041 1104 46-47 0.35165885422385657 1104 48-49 0.33297245761551153 1104 50-51 0.4435468221539196 1104 52-53 0.40387668757894346 1104 54-55 0.3934698104395409 1104 56-57 0.4144440440902031 1104 58-59 0.4726005753341198 1104 60-61 0.47447149133320465 1104 62-63 0.420485784712163 1104 64-65 0.43741666869610896 1104 66-67 0.42850661685230307 1104 68-69 0.4683091000694759 1104 70-71 0.4858966034955685 1104 72-73 0.48167682166405257 1104 74-75 0.4924270539845992 1104 76-77 0.5272673469939164 1104 78-79 0.44032758596692645 1104 80-81 0.4116780276540766 1104 82-83 0.36542325658189156 1104 84-85 0.3994140038620664 1104 86-87 0.42299332775096943 1104 88-89 0.3675088258343493 1104 90-91 0.3845120445807666 1104 92-93 0.442173021369058 1104 94-95 0.4318406440877247 1104 96-97 0.445740050200655 1104 98-99 0.4252005535525498 1104 100 0.38788980501076153 1105 1 0.1839696274407281 1105 2 0.1717036839087278 1105 3 0.2533892876881332 1105 4 0.2833313819293437 1105 5 0.267830649149694 1105 6 0.3380714024263085 1105 7 0.3261977779701297 1105 8 0.37873672704959915 1105 9 0.4204731169067273 1105 10-11 0.39013383994616646 1105 12-13 0.3715994464991894 1105 14-15 0.4864730858788491 1105 16-17 0.499233913640694 1105 18-19 0.46176117688600726 1105 20-21 0.44279908228845954 1105 22-23 0.42163332915745144 1105 24-25 0.44011019491797754 1105 26-27 0.37161891925873647 1105 28-29 0.3811670875795201 1105 30-31 0.38758788826628887 1105 32-33 0.4364148238994332 1105 34-35 0.4773230863452156 1105 36-37 0.4362397912815794 1105 38-39 0.42367686416147876 1105 40-41 0.495277379195457 1105 42-43 0.4342714799229199 1105 44-45 0.44610158670247557 1105 46-47 0.3385382459413435 1105 48-49 0.41152693151879305 1105 50-51 0.40078382244563926 1105 52-53 0.45670680467769387 1105 54-55 0.40993587994583436 1105 56-57 0.3661256088921263 1105 58-59 0.4257399101348085 1105 60-61 0.422189599228858 1105 62-63 0.4008196427173374 1105 64-65 0.41966418020043506 1105 66-67 0.47652552693077865 1105 68-69 0.4323577590782435 1105 70-71 0.4579371287811398 1105 72-73 0.3856473281463835 1105 74-75 0.4034669006022895 1105 76-77 0.5021513494241674 1105 78-79 0.43455423110034985 1105 80-81 0.4742847653460345 1105 82-83 0.444342348405641 1105 84-85 0.43200313679320956 1105 86-87 0.4758338063699341 1105 88-89 0.4562161160222189 1105 90-91 0.46780867988874775 1105 92-93 0.49725507338229846 1105 94-95 0.4672932521314834 1105 96-97 0.3992868523688742 1105 98-99 0.4797028048325629 1105 100 0.4999425124502679 1106 1 0.05170917133574093 1106 2 0.09322631679837556 1106 3 0.11273024130452214 1106 4 0.1417880695289142 1106 5 0.15205523950159971 1106 6 0.23845126812251038 1106 7 0.20542758468488387 1106 8 0.1773648409393047 1106 9 0.2423170077464718 1106 10-11 0.24724959051815887 1106 12-13 0.22193745733223125 1106 14-15 0.35435871618920345 1106 16-17 0.32971351459030274 1106 18-19 0.34566807314919856 1106 20-21 0.2996852782592754 1106 22-23 0.36137011930873086 1106 24-25 0.2933750610963486 1106 26-27 0.25199046046746076 1106 28-29 0.3145385797509661 1106 30-31 0.22368925268742856 1106 32-33 0.253462677898888 1106 34-35 0.3208125815219631 1106 36-37 0.20361967684790727 1106 38-39 0.2490439646713014 1106 40-41 0.29357373951769716 1106 42-43 0.27802290979698085 1106 44-45 0.2760186262073745 1106 46-47 0.27114428266678914 1106 48-49 0.2400620176885866 1106 50-51 0.2445534362160373 1106 52-53 0.29840475311490877 1106 54-55 0.350335630347935 1106 56-57 0.3147892565762618 1106 58-59 0.32473522479642725 1106 60-61 0.2800481779171875 1106 62-63 0.30719998912465485 1106 64-65 0.3473300077645973 1106 66-67 0.3018651693013865 1106 68-69 0.3481288574566861 1106 70-71 0.31472183217763927 1106 72-73 0.30963959848651257 1106 74-75 0.30055978777298975 1106 76-77 0.3602024313158694 1106 78-79 0.22636939958712787 1106 80-81 0.3289215586510039 1106 82-83 0.2735927817657142 1106 84-85 0.23717975483641496 1106 86-87 0.3095560654460705 1106 88-89 0.33601440875943567 1106 90-91 0.36229051449531724 1106 92-93 0.3480918197281575 1106 94-95 0.3750843316497061 1106 96-97 0.3593950296898427 1106 98-99 0.3882349806674661 1106 100 0.3920082814613721 1107 1 -0.026706911540646416 1107 2 -0.04875182296900604 1107 3 0.03273951360018401 1107 4 0.035118019776213316 1107 5 0.016565220464286767 1107 6 0.10746427040161421 1107 7 0.11483569199470622 1107 8 0.1731636031927124 1107 9 0.15776955607727672 1107 10-11 0.091247623410041 1107 12-13 0.11858698164339643 1107 14-15 0.07388397754435516 1107 16-17 0.18635630618054222 1107 18-19 0.11582137070428189 1107 20-21 0.07501498223382441 1107 22-23 0.12106089331125958 1107 24-25 0.09155535499087364 1107 26-27 0.09093596918568636 1107 28-29 0.18038124979601378 1107 30-31 0.17331065353638309 1107 32-33 0.1882996447045926 1107 34-35 0.15036274861043353 1107 36-37 0.15609287818664086 1107 38-39 0.284487373606936 1107 40-41 0.23664608430822653 1107 42-43 0.24849006949735752 1107 44-45 0.21165984441850583 1107 46-47 0.2497579940016763 1107 48-49 0.22549189699233807 1107 50-51 0.2687693526865331 1107 52-53 0.23944794695063365 1107 54-55 0.27672715502126977 1107 56-57 0.23558894777698924 1107 58-59 0.22646276847797253 1107 60-61 0.2062864601846357 1107 62-63 0.14222439083059157 1107 64-65 0.19638189835548303 1107 66-67 0.1979848998081124 1107 68-69 0.2873339560982302 1107 70-71 0.25258879462474226 1107 72-73 0.22808968580756073 1107 74-75 0.2685491443420389 1107 76-77 0.30768284589670003 1107 78-79 0.14867547719903484 1107 80-81 0.1469901401877678 1107 82-83 0.19553048994823286 1107 84-85 0.23398978135594106 1107 86-87 0.30032775351830665 1107 88-89 0.303220240071461 1107 90-91 0.2559185867682352 1107 92-93 0.28050364635322467 1107 94-95 0.21427636174271925 1107 96-97 0.1285917827228431 1107 98-99 0.18676400274495109 1107 100 0.12426586214620983 1108 1 -0.059626394400858374 1108 2 -0.01255399695740067 1108 3 0.06979521056193505 1108 4 -0.05750992017915024 1108 5 0.005277329003959608 1108 6 0.1213756561873538 1108 7 0.07410380567624486 1108 8 0.01781192372413898 1108 9 0.10505204044599026 1108 10-11 0.058324327857029346 1108 12-13 0.11251033295516066 1108 14-15 0.1817189708306941 1108 16-17 0.125900094128518 1108 18-19 0.08050908403799895 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-0.4695743932357175 2215 38-39 -0.3291797329619399 2215 40-41 -0.5674689023438546 2215 42-43 -0.39986946845125004 2215 44-45 -0.3687244335036084 2215 46-47 -0.4034471906462329 2215 48-49 -0.3491802135886175 2215 50-51 -0.3355139790152535 2215 52-53 -0.30180612466384815 2215 54-55 -0.3530556244447851 2215 56-57 -0.2808132708204809 2215 58-59 -0.25344309162984757 2215 60-61 -0.1044689026942649 2215 62-63 -0.14452033167570733 2215 64-65 -0.15069468815571696 2215 66-67 -0.1504973497072939 2215 68-69 -0.16845777618908642 2215 70-71 -0.1137901048792429 2215 72-73 -0.19271675704619895 2215 74-75 -0.15048686641925713 2215 76-77 -0.1758841792589223 2215 78-79 -0.1999334294702919 2215 80-81 -0.23397933245039582 2215 82-83 -0.24034516647062532 2215 84-85 -0.21034390154411042 2215 86-87 -0.10213305672543882 2215 88-89 -0.13638166747198 2215 90-91 -0.20198519163962558 2215 92-93 -0.20127374602436987 2215 94-95 -0.2799273891954215 2215 96-97 -0.18296381376292814 2215 98-99 -0.3553783825092047 2215 100 -0.29105723984332244 2216 1 -1.0396800753824529 2216 2 -1.0714665736445141 2216 3 -1.1685192066159793 2216 4 -1.3984708097497034 2216 5 -1.3233492047383706 2216 6 -2.6354364603770613 2216 7 -2.5163984949372527 2216 8 -3.336859855416968 2216 9 -2.853949493296348 2216 10-11 -2.0471722212227306 2216 12-13 -1.8146238694988313 2216 14-15 -2.074091611991385 2216 16-17 -2.400722221822406 2216 18-19 -2.0377581705632224 2216 20-21 -1.9348716396035641 2216 22-23 -2.055084817202129 2216 24-25 -2.1823766488165433 2216 26-27 -2.3037478155109454 2216 28-29 -2.693956643227807 2216 30-31 -2.3006866597772984 2216 32-33 -2.496136903440828 2216 34-35 -3.4190510778449834 2216 36-37 -2.5473517453702215 2216 38-39 -2.4510724857642288 2216 40-41 -2.954207514316195 2216 42-43 -2.697489788031511 2216 44-45 -2.508889559904752 2216 46-47 -2.3530460160695625 2216 48-49 -2.5655924035480737 2216 50-51 -2.8328137196544105 2216 52-53 -3.2026643258324583 2216 54-55 -2.6002109130209803 2216 56-57 -2.608900827462506 2216 58-59 -3.6012617924407238 2216 60-61 -4.311490068923693 2216 62-63 -3.4503268097343778 2216 64-65 -3.3942595134597937 2216 66-67 -3.081291306897853 2216 68-69 -3.44213926915355 2216 70-71 -3.195930472754277 2216 72-73 -3.6547477312889782 2216 74-75 -4.099873955019305 2216 76-77 -4.737940403919069 2216 78-79 -3.6982857577555244 2216 80-81 -3.568101038269617 2216 82-83 -3.737220554021306 2216 84-85 -3.813006180938334 2216 86-87 -3.452043577235809 2216 88-89 -3.4171387626305787 2216 90-91 -3.5493430350691533 2216 92-93 -3.648640667083278 2216 94-95 -3.4953472258279064 2216 96-97 -3.811948103054604 2216 98-99 -3.6315619920966142 2216 100 -3.3199330447562723 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 89205.0 3 15840.0 4 10344.0 5 8260.0 6 7519.0 7 7681.0 8 8328.0 9 9554.0 10 10777.0 11 12244.0 12 13380.0 13 15084.0 14 16803.0 15 17681.0 16 18190.0 17 19338.0 18 17977.0 19 16516.0 20 16771.0 21 17851.0 22 19692.0 23 23225.0 24 28172.0 25 34803.0 26 42721.0 27 50074.0 28 61645.0 29 76709.0 30 92663.0 31 110540.0 32 141585.0 33 185056.0 34 252799.0 35 362738.0 36 587748.0 37 1009656.0 38 1306694.0 39 457571.0 40 1044.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.44838972952586 20.242693370407743 14.184311956439494 23.12460494362691 2 15.15882697868153 20.13071656285535 40.82943780131198 23.881018657151138 3 18.186410160124016 25.347757012045406 30.367062403111973 26.098770424718605 4 12.518041998981271 16.743225403904873 38.998217705664345 31.74051489144951 5 13.039672689001591 38.03611475143064 35.62008737408801 13.304125185479762 6 30.92621736608453 37.89383900640218 17.7152349765166 13.464708650996688 7 26.930933392236728 32.75431987664181 22.57821245779879 17.736534273322675 8 24.74372667156842 36.71661629465068 20.541768460249372 17.997888573531526 9 24.75336549127391 17.166489751774797 21.69682693255768 36.383317824393615 10-11 23.74141244049804 27.21702217311041 29.011382548453728 20.030182837937822 12-13 24.00019758741149 24.708585047851876 29.186356801075213 22.104860563661422 14-15 21.465007913520058 26.9120536723172 26.258096589719155 25.364841824443584 16-17 21.135854173573186 30.335912291852356 26.96653490558235 21.561698628992104 18-19 21.08531943212623 29.3441890054737 28.856517615897666 20.71397394650241 20-21 22.08458194606609 28.509100068691755 28.474715676413503 20.931602308828655 22-23 21.78261189829795 28.28297514740596 28.56495659546278 21.369456358833308 24-25 21.636005012678535 28.407222840328473 28.45963161723395 21.497140529759047 26-27 21.368719024016464 28.444528406312276 28.53245727982613 21.65429528984513 28-29 21.434938761361884 28.49651440456016 28.640405946695424 21.42814088738253 30-31 21.642443066853513 28.72484426035198 28.314478062995292 21.31823460979922 32-33 21.3972637376636 28.534964716140877 28.577070984736885 21.490700561458638 34-35 21.578147579271054 28.310034478402468 28.599617240433705 21.51220070189277 36-37 21.716271988575222 28.557225177953693 28.216540430786203 21.509962402684884 38-39 21.573664666698892 28.706293591829024 28.317581428703754 21.402460312768333 40-41 21.535662277429715 28.512182318239642 28.416753099547954 21.53540230478269 42-43 21.472717174953935 28.501757411709605 28.396256863264664 21.62926855007179 44-45 21.57370525792202 28.56028977165337 28.310256866252324 21.555748104172288 46-47 21.573195854848045 28.63098449862294 28.179228485961183 21.61659116056783 48-49 21.544390428956152 28.54004126744792 28.211066724052102 21.704501579543827 50-51 21.597651567006896 28.50714036129457 28.156065496616105 21.739142575082425 52-53 21.576153770310473 28.50715247469231 28.213057936161917 21.7036358188353 54-55 21.629121444398624 28.728023785482353 28.082923744859706 21.559931025259317 56-57 21.55560311962527 28.58460015996548 28.104222312536187 21.755574407873066 58-59 21.55197348100003 28.2642487246185 28.3449638317752 21.838813962606267 60-61 21.674394573977867 28.561669509128247 28.111070425808865 21.652865491085024 62-63 21.521548132161428 28.612917373351554 28.11195140350869 21.753583090978328 64-65 21.58217655643756 28.384394425441638 28.192399019235744 21.84102999888506 66-67 21.4627180645188 28.453751936845524 28.10910825046849 21.974421748167188 68-69 21.699774909267582 28.503863938824004 28.074855137196742 21.72150601471168 70-71 21.688560152283532 28.356346928380717 28.118004330875834 21.83708858845992 72-73 21.539968126864018 28.46516369451086 28.204904288161494 21.789963890463632 74-75 21.57448235536409 28.493950249616535 28.088043565019543 21.84352382999983 76-77 21.63482641988995 28.457118857962527 28.006736716268687 21.901318005878835 78-79 21.650199214465026 28.4901381594511 28.097264761950246 21.762397864133625 80-81 21.622124636037647 28.39286183666083 28.128362388218868 21.85665113908265 82-83 21.508169143617717 28.4533950770622 28.241093239885863 21.797342539434226 84-85 21.533280621572565 28.348348996018117 28.084600835505803 22.033769546903518 86-87 21.664625139153483 28.464384892991674 28.038109711772 21.83288025608284 88-89 21.55006091874362 28.321053621781545 28.142996039078117 21.985889420396713 90-91 21.533895299060536 28.289105523976488 28.140067267967684 22.036931908995292 92-93 21.57430096273181 28.438581677188772 28.035233731334376 21.95188362874504 94-95 21.520663534679965 28.25950589223733 28.160891927779762 22.058938645302938 96-97 21.547641553203228 28.31548232565428 28.093823864496102 22.043052256646384 98-99 21.54506189072319 28.35458153831819 28.03996859742211 22.060387973536514 100 21.583670197467384 28.215173882727 28.073908485125937 22.127247434679674 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1419.0 1 1208.0 2 1195.0 3 1386.5 4 1271.0 5 1042.0 6 841.5 7 760.5 8 775.5 9 784.5 10 724.0 11 709.0 12 758.5 13 823.0 14 914.5 15 1035.5 16 1290.5 17 1647.5 18 2181.5 19 3079.0 20 4409.0 21 6352.0 22 9482.0 23 14256.0 24 20575.0 25 28470.0 26 38733.0 27 50904.0 28 65356.5 29 81608.0 30 96941.5 31 110916.5 32 123011.0 33 134462.0 34 145524.0 35 155660.0 36 164407.0 37 172682.0 38 180809.0 39 184975.5 40 187527.0 41 189816.0 42 189906.0 43 188448.5 44 185801.0 45 182440.5 46 179040.0 47 173108.5 48 165701.0 49 160357.0 50 155764.5 51 149209.0 52 139435.5 53 130894.0 54 124255.0 55 118123.0 56 109945.0 57 99101.0 58 89442.0 59 81072.5 60 71432.0 61 60351.5 62 51165.0 63 43255.5 64 35442.0 65 28537.5 66 22655.5 67 17622.5 68 13505.5 69 10445.5 70 7842.0 71 5647.5 72 4203.5 73 3047.5 74 2172.5 75 1530.0 76 1024.5 77 647.5 78 428.0 79 325.0 80 246.5 81 182.5 82 140.0 83 105.0 84 78.0 85 62.5 86 53.0 87 46.5 88 35.5 89 26.5 90 25.0 91 15.5 92 15.5 93 20.0 94 16.5 95 11.5 96 9.0 97 7.5 98 6.0 99 6.5 100 17.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011531476310035387 2 0.030089645196302688 3 0.03134097401124809 4 0.033362351327698374 5 0.03586500895758919 6 0.03607677229550303 7 0.03455592650503092 8 0.0342671583169666 9 0.034151651041740866 10-11 0.034498172867418055 12-13 0.035412605462955084 14-15 0.034315286348310645 16-17 0.031177338704678315 18-19 0.03348748420919292 20-21 0.033015829502021185 22-23 0.03389175967248297 24-25 0.03167787023065648 26-27 0.03165861901811885 28-29 0.03230353463812918 30-31 0.03228428342559156 32-33 0.03204364326887129 34-35 0.031995515237527236 36-37 0.02991638428346409 38-39 0.030051142771227446 40-41 0.03126396916109762 42-43 0.031071457035721395 44-45 0.030060768377496258 46-47 0.029261843057184957 48-49 0.029319596694797823 50-51 0.030195526865259607 52-53 0.030609427934818474 54-55 0.03008001959003388 56-57 0.028539922587024143 58-59 0.029300345482260202 60-61 0.028924946837776577 62-63 0.02907895653807755 64-65 0.02666292936460603 66-67 0.02759661317268068 68-69 0.028126021517465277 70-71 0.029512108820174035 72-73 0.026759185427294135 74-75 0.027192337709390627 76-77 0.0277891252980569 78-79 0.02803939106104598 80-81 0.02813564712373409 82-83 0.026951697552670354 84-85 0.028029765454777172 86-87 0.028318533642841495 88-89 0.028780562743744416 90-91 0.02918483820703447 92-93 0.027779499691788088 94-95 0.028905695625238953 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5194478.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.734951570331695 #Duplication Level Percentage of deduplicated Percentage of total 1 77.43495098092788 48.57877899639519 2 13.480336139593415 16.9137646973837 3 4.102704105563247 7.721488301097339 4 1.7748645628116781 4.453841695675543 5 0.9086251842601983 2.8501278465073625 6 0.5409382779791791 2.0361442002937458 7 0.3480127593230947 1.5282794521394296 8 0.2491028054083026 1.25019619466589 9 0.18322222148561249 1.0344993472357638 >10 0.9153308034748172 10.147329590973118 >50 0.04783760580022581 2.0513414501051366 >100 0.013920898137812257 1.34049277369235 >500 9.219353846540779E-5 0.04021627701241613 >1k 6.146169609279098E-5 0.053499176822970715 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.9251212537621684E-5 0.0 5 0.0 1.9251212537621684E-5 0.0 1.9251212537621684E-5 0.0 6 0.0 1.9251212537621684E-5 0.0 1.9251212537621684E-5 0.0 7 0.0 1.9251212537621684E-5 0.0 1.9251212537621684E-5 0.0 8 0.0 1.9251212537621684E-5 0.0 5.775363761286505E-5 0.0 9 0.0 3.850242507524337E-5 0.0 5.775363761286505E-5 0.0 10-11 0.0 5.775363761286505E-5 0.0 7.700485015048673E-5 0.0 12-13 0.0 7.700485015048673E-5 0.0 9.625606268810842E-5 0.0 14-15 0.0 1.4438409403216262E-4 0.0 1.4438409403216262E-4 0.0 16-17 0.0 2.117633379138385E-4 0.0 1.636353065697843E-4 0.0 18-19 0.0 2.117633379138385E-4 0.0 1.7326091283859514E-4 0.0 20-21 0.0 2.117633379138385E-4 0.0 2.0213773164502768E-4 0.0 22-23 0.0 2.2138894418264934E-4 0.0 2.9839379433313604E-4 0.0 24-25 0.0 2.40640156720271E-4 0.0 4.427778883652987E-4 0.0 26-27 0.0 2.598913692578927E-4 0.0 8.085509265801107E-4 0.0 28-29 0.0 2.6951697552670355E-4 0.0 0.0025507856612348727 0.0 30-31 0.0 2.791425817955144E-4 0.0 0.007219204701608131 0.0 32-33 0.0 2.8876818806432525E-4 0.0 0.01763411068446146 0.0 34-35 0.0 3.1764500687075773E-4 0.0 0.03367999633456913 0.0 36-37 0.0 3.753986444836228E-4 0.0 0.059014592034079265 0.0 38-39 0.0 4.3315228209648784E-4 0.0 0.09218443123640142 0.0 40-41 0.0 4.427778883652987E-4 0.0 0.12442058663064894 0.0 42-43 0.0 5.197827385157854E-4 0.0 0.15882250343537888 0.0 44-45 0.0 5.294083447845963E-4 0.0 0.1998660885655883 0.0 46-47 0.0 5.582851635910288E-4 0.0 0.24992886676967346 0.0 48-49 0.0 5.967875886662722E-4 0.0 0.3026964403352945 0.0 50-51 0.0 6.160388012038939E-4 0.0 0.3537699071976048 0.0 52-53 0.0 6.160388012038939E-4 0.0 0.40261985901181985 0.0 54-55 0.0 6.160388012038939E-4 0.0 0.45708153928075157 0.0 56-57 0.0 6.160388012038939E-4 0.0 0.5241238869430191 0.0 58-59 0.0 6.160388012038939E-4 0.0 0.5948432161999724 0.0 60-61 0.0 6.160388012038939E-4 0.0 0.6692202758390737 0.0 62-63 0.0 6.160388012038939E-4 0.0 0.7401321172214033 0.0 64-65 0.0 6.160388012038939E-4 0.0 0.817031085702933 0.0 66-67 0.0 6.160388012038939E-4 0.0 0.901861553749963 0.0 68-69 0.0 6.160388012038939E-4 0.0 0.9982908773509099 9.625606268810842E-6 70-71 0.0 6.160388012038939E-4 0.0 1.0978581485954892 1.9251212537621684E-5 72-73 0.0 6.352900137415155E-4 0.0 1.196799755432596 1.9251212537621684E-5 74-75 0.0 6.545412262791372E-4 0.0 1.3011413273865053 1.9251212537621684E-5 76-77 0.0 6.64166832547948E-4 0.0 1.4120090603906688 1.9251212537621684E-5 78-79 0.0 6.930436513543805E-4 0.0 1.5361312532269844 1.9251212537621684E-5 80-81 0.0 6.930436513543805E-4 0.0 1.6742298263656137 1.9251212537621684E-5 82-83 0.0 6.930436513543805E-4 0.0 1.8114332181212434 1.9251212537621684E-5 84-85 0.0 6.930436513543805E-4 0.0 1.9495702936849477 1.9251212537621684E-5 86-87 0.0 6.930436513543805E-4 0.0 2.0961490259463993 1.9251212537621684E-5 88 0.0 6.930436513543805E-4 0.0 2.2123685960360215 1.9251212537621684E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5135 0.0 47.23446 1 GTATCAA 8790 0.0 34.438694 1 TCAACGC 9280 0.0 31.454657 4 ATCAACG 9590 0.0 30.388859 3 CAACGCA 10000 0.0 29.096191 5 AACGCAG 10540 0.0 27.514711 6 TATCAAC 10940 0.0 27.111486 2 GTACATG 14315 0.0 25.612558 1 TACATGG 14505 0.0 24.20718 2 ACGCAGA 12285 0.0 23.147093 7 CGCAGAG 12375 0.0 22.560953 8 TAGACCG 230 4.101821E-9 22.480726 5 ACATGGG 14710 0.0 22.463839 3 CATGGGG 10990 0.0 19.246695 4 GCAGAGT 14405 0.0 18.598675 9 GTGGTAT 3035 0.0 18.585413 1 ACCGCGT 315 7.772542E-9 17.90552 8 GAGTACA 10705 0.0 17.168793 1 AGAGTAC 12230 0.0 16.85247 10-11 CAGAGTA 13700 0.0 16.793938 10-11 >>END_MODULE