FastQCFastQC Report
Wed 25 May 2016
SRR2049351_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049351_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5194478
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130610.25144008695387676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120880.23270865715477088No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71870.13835846450788702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85050.055.7209361
GTATCAA155250.041.6392821
ATCAACG189550.033.5703353
TCAACGC189850.033.5420384
CAACGCA196200.032.4325035
TATCAAC199650.032.036832
AACGCAG203050.031.3868966
ACGCAGA233950.027.0201827
GTGGTAT38550.026.9525621
CGCAGAG237200.026.5111628
GTACATG144350.024.1993791
GCAGAGT257550.023.9963879
TGGTATC41300.023.5548882
TACATGG147800.022.6076762
GAGTACT178650.021.72453312-13
ACATGGG152300.021.291683
CAGAGTA250300.021.03658910-11
AGAGTAC238000.020.9182810-11
AGTACTT187800.019.66468412-13
GTACTTT197200.019.61343814-15
Produced by FastQC (version 0.11.5)