##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2049351_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5194478 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.876593182221583 33.0 31.0 34.0 30.0 34.0 2 31.985234127471518 33.0 31.0 34.0 30.0 34.0 3 31.97235583633235 33.0 31.0 34.0 30.0 34.0 4 35.689011484888375 37.0 35.0 37.0 33.0 37.0 5 35.77641564753956 37.0 35.0 37.0 35.0 37.0 6 35.82856448713422 37.0 35.0 37.0 35.0 37.0 7 35.77391279739754 37.0 35.0 37.0 33.0 37.0 8 35.7632195188814 37.0 35.0 37.0 33.0 37.0 9 37.4662818862646 39.0 37.0 39.0 34.0 39.0 10-11 37.4414658219748 39.0 37.0 39.0 34.5 39.0 12-13 37.39601678936748 39.0 37.0 39.0 34.0 39.0 14-15 38.68805710602682 40.0 38.0 41.0 34.0 41.0 16-17 38.713479872279756 40.0 38.0 41.0 34.0 41.0 18-19 38.78217349269744 40.0 38.0 41.0 35.0 41.0 20-21 38.81341156897767 40.0 38.0 41.0 35.0 41.0 22-23 38.78616513536105 40.0 38.0 41.0 35.0 41.0 24-25 38.771587828459374 40.0 38.0 41.0 35.0 41.0 26-27 38.65984368400444 40.0 38.0 41.0 34.0 41.0 28-29 38.5799689593449 40.0 38.0 41.0 34.0 41.0 30-31 38.482750817310226 40.0 38.0 41.0 34.0 41.0 32-33 38.354277272904035 40.0 38.0 41.0 34.0 41.0 34-35 38.261930553945945 40.0 38.0 41.0 34.0 41.0 36-37 38.16106979373096 40.0 38.0 41.0 34.0 41.0 38-39 38.07763494233684 40.0 38.0 41.0 33.5 41.0 40-41 37.972221462868845 40.0 37.5 41.0 33.0 41.0 42-43 37.84144027561576 40.0 37.0 41.0 33.0 41.0 44-45 37.67234599896275 40.0 37.0 41.0 33.0 41.0 46-47 37.450519859743366 40.0 37.0 41.0 33.0 41.0 48-49 37.25019183833294 40.0 37.0 41.0 32.0 41.0 50-51 37.106940774414674 40.0 37.0 41.0 31.5 41.0 52-53 36.96970465559774 40.0 36.0 41.0 31.0 41.0 54-55 36.83444034222495 40.0 36.0 41.0 31.0 41.0 56-57 36.6927972358339 39.0 36.0 41.0 31.0 41.0 58-59 36.50500435269916 39.0 35.0 41.0 31.0 41.0 60-61 36.3324432214363 39.0 35.0 41.0 31.0 41.0 62-63 36.13531398150113 39.0 35.0 41.0 30.0 41.0 64-65 35.91265850004562 38.0 35.0 40.0 30.0 41.0 66-67 35.649383441416056 38.0 35.0 40.0 30.0 41.0 68-69 35.366969886098275 37.0 35.0 40.0 29.5 41.0 70-71 35.04105802353961 37.0 34.0 39.5 29.0 41.0 72-73 34.748776104162914 36.0 34.0 39.0 29.0 41.0 74-75 34.441101589033586 36.0 34.0 39.0 29.0 40.5 76-77 34.11857947997855 35.5 34.0 38.5 29.0 40.0 78-79 33.782317491767216 35.0 34.0 37.0 29.0 39.0 80-81 33.46810179964185 35.0 34.0 37.0 29.0 39.0 82-83 33.17065757136713 35.0 34.0 36.5 28.5 39.0 84-85 32.90097927067936 35.0 33.5 36.0 28.0 37.0 86-87 32.666570827713585 35.0 33.0 36.0 27.5 37.0 88-89 32.44254263854809 35.0 33.0 35.0 27.0 37.0 90-91 32.2472200286535 35.0 33.0 35.0 27.0 36.0 92-93 32.06672114118108 35.0 33.0 35.0 27.0 36.0 94-95 31.912169808015356 35.0 33.0 35.0 26.5 36.0 96-97 31.717244061867238 35.0 33.0 35.0 26.0 36.0 98-99 31.56890519124347 35.0 33.0 35.0 25.5 35.0 100 31.455918573531353 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10668221623177843 1101 2 0.10795599281757617 1101 3 0.2730298920197036 1101 4 0.1483947266652521 1101 5 0.10043634464626194 1101 6 0.14240119955911723 1101 7 0.0318259197204398 1101 8 0.11657500713625524 1101 9 0.2028380046205811 1101 10-11 0.17374623037916592 1101 12-13 0.09564332053965785 1101 14-15 0.28697758849429533 1101 16-17 0.15938781667898638 1101 18-19 0.23033188230940027 1101 20-21 0.2808712956416457 1101 22-23 0.20079176436041735 1101 24-25 0.1658604358307869 1101 26-27 0.2822173526392149 1101 28-29 0.2916561141637146 1101 30-31 0.23938771119234303 1101 32-33 0.241647956416692 1101 34-35 0.27200985975016323 1101 36-37 0.22356592920449714 1101 38-39 0.20663845286981086 1101 40-41 0.22379569322792747 1101 42-43 0.27779033944471365 1101 44-45 0.23781065104886778 1101 46-47 0.3212381979510468 1101 48-49 0.32573624197291906 1101 50-51 0.2627987899444406 1101 52-53 0.29539994338874465 1101 54-55 0.31479128147962143 1101 56-57 0.2506743610594384 1101 58-59 0.29611474069475463 1101 60-61 0.3651965720145469 1101 62-63 0.3323123303614963 1101 64-65 0.27305476910528625 1101 66-67 0.2697972712053698 1101 68-69 0.2857950776749263 1101 70-71 0.30277440882293405 1101 72-73 0.23928722756456722 1101 74-75 0.2951615043799194 1101 76-77 0.22342405820454303 1101 78-79 0.28416906881783177 1101 80-81 0.3325631453228226 1101 82-83 0.2400806485043887 1101 84-85 0.2631970033857627 1101 86-87 0.1953248654110169 1101 88-89 0.30658282196763764 1101 90-91 0.3299003702858627 1101 92-93 0.2986586222110361 1101 94-95 0.36343142847800536 1101 96-97 0.3378333203730861 1101 98-99 0.3402929417624563 1101 100 0.3324826634061573 1102 1 0.19330916708577917 1102 2 0.21630487499108497 1102 3 0.3952795488299934 1102 4 0.21181297682579725 1102 5 0.2033278198327153 1102 6 0.1767069595014945 1102 7 0.16214083070214969 1102 8 0.15433330446919769 1102 9 0.14473758647902457 1102 10-11 0.2101759147063973 1102 12-13 0.20098798663979522 1102 14-15 0.2591919955477522 1102 16-17 0.23448438248279757 1102 18-19 0.25757798840798785 1102 20-21 0.2702312221670553 1102 22-23 0.28659696437297555 1102 24-25 0.2507342924870315 1102 26-27 0.32530984151568276 1102 28-29 0.33382640454655643 1102 30-31 0.27959627411235033 1102 32-33 0.3056109264141682 1102 34-35 0.2817965099320574 1102 36-37 0.2791804974340124 1102 38-39 0.24891945957335793 1102 40-41 0.24332621176223768 1102 42-43 0.32036375013020546 1102 44-45 0.2532753430428585 1102 46-47 0.245871747175201 1102 48-49 0.3039013209850481 1102 50-51 0.3765215400122557 1102 52-53 0.39905775978426306 1102 54-55 0.29213114386745076 1102 56-57 0.35812288603426623 1102 58-59 0.3442995969737126 1102 60-61 0.3682076167095971 1102 62-63 0.3537332961859718 1102 64-65 0.2877518245109698 1102 66-67 0.2955156362000011 1102 68-69 0.36180925238367223 1102 70-71 0.39976695001212903 1102 72-73 0.35886694477384395 1102 74-75 0.3550270783298686 1102 76-77 0.28375134477622055 1102 78-79 0.3102018163480764 1102 80-81 0.32879529890023207 1102 82-83 0.3273309684459065 1102 84-85 0.2645190022365611 1102 86-87 0.24720319103984423 1102 88-89 0.21801897890171773 1102 90-91 0.2681361392581394 1102 92-93 0.3229816149865954 1102 94-95 0.3544816275975258 1102 96-97 0.4334202978827406 1102 98-99 0.4171030798292925 1102 100 0.41183992882799103 1103 1 0.16015949546932262 1103 2 0.16756104167930275 1103 3 0.27824764886625175 1103 4 0.1538068918658837 1103 5 0.10435386533860225 1103 6 0.09229577230357933 1103 7 0.09115543105095014 1103 8 0.05840875974151771 1103 9 0.17086639749378207 1103 10-11 0.17650809670342937 1103 12-13 0.1705374992474944 1103 14-15 0.22358729661466725 1103 16-17 0.19997197997210492 1103 18-19 0.19781980097670981 1103 20-21 0.19482230419409063 1103 22-23 0.19330181432832205 1103 24-25 0.252258882692594 1103 26-27 0.22707546775844634 1103 28-29 0.24608883648434698 1103 30-31 0.225131525079874 1103 32-33 0.2506925207906434 1103 34-35 0.20392492219623648 1103 36-37 0.19688037013362703 1103 38-39 0.23270932326504123 1103 40-41 0.2334085855371626 1103 42-43 0.24674307447079258 1103 44-45 0.2911859637741401 1103 46-47 0.22335122745280955 1103 48-49 0.20263937536240917 1103 50-51 0.1844602395660644 1103 52-53 0.17978645105917224 1103 54-55 0.20967243376001932 1103 56-57 0.09522630717163594 1103 58-59 0.2627840122401537 1103 60-61 0.15879650528084 1103 62-63 0.1253785346511762 1103 64-65 0.18381751285168946 1103 66-67 0.18274619799024805 1103 68-69 0.20958176600225187 1103 70-71 0.22738228644400493 1103 72-73 0.20362535295959816 1103 74-75 0.2392370881651118 1103 76-77 0.15737672221928278 1103 78-79 0.17280689281636086 1103 80-81 0.23361425033203886 1103 82-83 0.15897061391831357 1103 84-85 0.11198164865432148 1103 86-87 0.07863153475136642 1103 88-89 0.1548617868888229 1103 90-91 0.19800063205159546 1103 92-93 0.22141553604561892 1103 94-95 0.1238786107398262 1103 96-97 0.1765307718593867 1103 98-99 0.15581981890806773 1103 100 0.23181770921422995 1104 1 0.21269200605764382 1104 2 0.16087675908832821 1104 3 0.24125055538322826 1104 4 0.17974639888495858 1104 5 0.15114344298974913 1104 6 0.17267472661330885 1104 7 0.2068417816059238 1104 8 0.17694398937144484 1104 9 0.19782461198498424 1104 10-11 0.18821625617189852 1104 12-13 0.19617994199177247 1104 14-15 0.22266691639225655 1104 16-17 0.2411728357107421 1104 18-19 0.28492865319894634 1104 20-21 0.27870654966484665 1104 22-23 0.2709120187424787 1104 24-25 0.28924398563368214 1104 26-27 0.30633816428859717 1104 28-29 0.3410409609016085 1104 30-31 0.35326203170822623 1104 32-33 0.32093541424793415 1104 34-35 0.2892864552977983 1104 36-37 0.2831666807906146 1104 38-39 0.3043867244703833 1104 40-41 0.30070590665145147 1104 42-43 0.31931322238506255 1104 44-45 0.2481682886260046 1104 46-47 0.22839133201098605 1104 48-49 0.13293793056537595 1104 50-51 0.13324334854010544 1104 52-53 0.1801913421045498 1104 54-55 0.07150848673569499 1104 56-57 0.12188206685048186 1104 58-59 0.2341070492783146 1104 60-61 0.21070251205536294 1104 62-63 0.2048317182009356 1104 64-65 0.1376698897527575 1104 66-67 0.22225059629243304 1104 68-69 0.2197981082186331 1104 70-71 0.1972573113562177 1104 72-73 0.20618055586880502 1104 74-75 0.2114373490863528 1104 76-77 0.17237284527738694 1104 78-79 0.17316667315219547 1104 80-81 0.2003710775439771 1104 82-83 0.19717758271875852 1104 84-85 0.21451359776168744 1104 86-87 0.23438869053779143 1104 88-89 0.20934531163589298 1104 90-91 0.20184122586231723 1104 92-93 0.3100629982694372 1104 94-95 0.3461289123496165 1104 96-97 0.341807272555009 1104 98-99 0.37306108564050433 1104 100 0.37627133225699794 1105 1 0.18298877870839192 1105 2 0.15227318888736718 1105 3 0.30137838707210207 1105 4 0.19738030293925135 1105 5 0.14195686770280247 1105 6 0.12819255656761186 1105 7 0.20515530970028095 1105 8 0.15751719147694843 1105 9 0.20617342444717934 1105 10-11 0.21042342651230683 1105 12-13 0.13915551425174755 1105 14-15 0.20457822814107374 1105 16-17 0.2342639237180748 1105 18-19 0.2000250232894274 1105 20-21 0.26789658378161363 1105 22-23 0.22970607718949054 1105 24-25 0.227575749068059 1105 26-27 0.275488807788129 1105 28-29 0.2555391929409083 1105 30-31 0.25667813838501274 1105 32-33 0.29348791090184534 1105 34-35 0.2872993121901857 1105 36-37 0.2122467527477454 1105 38-39 0.2753641063242682 1105 40-41 0.2844939399797113 1105 42-43 0.28619268146412224 1105 44-45 0.30535646580865716 1105 46-47 0.3544302673762374 1105 48-49 0.3167934704843134 1105 50-51 0.32359027562662845 1105 52-53 0.3316968592983187 1105 54-55 0.32922515290650267 1105 56-57 0.3092745339176872 1105 58-59 0.33020119119755265 1105 60-61 0.316509023786665 1105 62-63 0.274367997368266 1105 64-65 0.27694680159123664 1105 66-67 0.34278667562042386 1105 68-69 0.2926945605650886 1105 70-71 0.3325235664062305 1105 72-73 0.36585764037745605 1105 74-75 0.35231068350121575 1105 76-77 0.3526592040155805 1105 78-79 0.37412756264684077 1105 80-81 0.36924905167450106 1105 82-83 0.37539711054030533 1105 84-85 0.370199415324457 1105 86-87 0.3033816097134405 1105 88-89 0.3227559161817197 1105 90-91 0.37668918984932276 1105 92-93 0.41582511685088974 1105 94-95 0.3692952366884903 1105 96-97 0.42356913984034605 1105 98-99 0.4396573828495072 1105 100 0.4859624551052377 1106 1 0.11239939442150515 1106 2 0.14685320286207926 1106 3 0.23619723080233612 1106 4 0.16498010883184833 1106 5 0.1110574147145087 1106 6 0.10736123986939816 1106 7 0.11712301145448833 1106 8 0.11064712767562668 1106 9 0.1599962628011795 1106 10-11 0.11276991022072025 1106 12-13 0.1061922118155465 1106 14-15 0.09283718250232198 1106 16-17 0.12644731838445722 1106 18-19 0.19658851753612083 1106 20-21 0.19339025605913207 1106 22-23 0.15198443554550778 1106 24-25 0.19931694874583883 1106 26-27 0.23822175534941437 1106 28-29 0.20327092733096208 1106 30-31 0.21106187474458693 1106 32-33 0.17848766389252546 1106 34-35 0.17013032791984983 1106 36-37 0.16793895489142585 1106 38-39 0.23734091825610193 1106 40-41 0.1698018621610231 1106 42-43 0.20549977292848354 1106 44-45 0.18772477201562765 1106 46-47 0.24684804745336208 1106 48-49 0.24051086320342563 1106 50-51 0.20836548712037484 1106 52-53 0.2500576134004433 1106 54-55 0.20348243872435745 1106 56-57 0.19383427307584356 1106 58-59 0.21187784852525482 1106 60-61 0.14472434226553332 1106 62-63 0.20113142059293665 1106 64-65 0.20696206603794565 1106 66-67 0.17628883663294204 1106 68-69 0.2589884527993931 1106 70-71 0.3281375559202715 1106 72-73 0.35480212763076935 1106 74-75 0.2240629666967564 1106 76-77 0.18737982280399734 1106 78-79 0.22095824623556126 1106 80-81 0.2581965559051156 1106 82-83 0.2641593122081929 1106 84-85 0.20903998548394753 1106 86-87 0.23948847943453444 1106 88-89 0.20516936311381784 1106 90-91 0.20392699857223562 1106 92-93 0.24036314106436407 1106 94-95 0.21565108815696377 1106 96-97 0.2275039663860312 1106 98-99 0.25304368828829027 1106 100 0.25623312445309665 1107 1 0.10550791617293243 1107 2 0.05144330564479205 1107 3 0.17894451183453342 1107 4 0.1279670501378405 1107 5 0.13279473697930655 1107 6 0.1334516409549522 1107 7 0.046014421535055305 1107 8 0.11885920286982099 1107 9 0.10873505067815614 1107 10-11 0.09852254786607517 1107 12-13 0.12197769396452429 1107 14-15 0.14995711398714207 1107 16-17 0.07647031378618863 1107 18-19 0.10021789440367002 1107 20-21 0.10963756290295379 1107 22-23 0.06269207462068493 1107 24-25 0.043117755497036114 1107 26-27 0.17682360328105773 1107 28-29 0.1790869702648763 1107 30-31 0.10590191815151684 1107 32-33 0.09065040515778122 1107 34-35 0.08599016653887048 1107 36-37 0.15344369243238276 1107 38-39 0.09018899391572432 1107 40-41 0.06790434146041946 1107 42-43 0.17460332383981125 1107 44-45 0.1616622845541542 1107 46-47 0.21808853885248425 1107 48-49 0.24811919044097408 1107 50-51 0.2945023880092492 1107 52-53 0.2702563638284232 1107 54-55 0.23686487291352876 1107 56-57 0.23681578444737994 1107 58-59 0.26394484899818593 1107 60-61 0.21586656414660865 1107 62-63 0.19781467179466716 1107 64-65 0.1928504972488838 1107 66-67 0.2357897437466221 1107 68-69 0.2143563308585712 1107 70-71 0.1898853538178571 1107 72-73 0.2164987769200195 1107 74-75 0.15552543627542548 1107 76-77 0.17758315784696066 1107 78-79 0.14425712217765607 1107 80-81 0.2558584565069282 1107 82-83 0.2810940671970883 1107 84-85 0.2344591486683072 1107 86-87 0.19307483541781778 1107 88-89 0.24733780825127383 1107 90-91 0.216890311617 1107 92-93 0.2699951368853206 1107 94-95 0.3017011516824972 1107 96-97 0.3341056221091989 1107 98-99 0.35729707365713637 1107 100 0.3951910326969674 1108 1 0.061477643693489625 1108 2 0.09864833056292 1108 3 0.1740138410567944 1108 4 0.07477751409583533 1108 5 0.05865094270196636 1108 6 0.04170927048478745 1108 7 0.022017815283746245 1108 8 -0.023356107276917726 1108 9 0.0844902125066227 1108 10-11 0.05455584035988892 1108 12-13 0.03778765006581608 1108 14-15 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2215 3 -0.2937649430399851 2215 4 -0.2159500642958463 2215 5 -0.17042415909638464 2215 6 -0.22415147609986263 2215 7 -0.15807052944505529 2215 8 -0.20586900124714447 2215 9 -0.4069053796210653 2215 10-11 -0.33333105556217646 2215 12-13 -0.2999803506482053 2215 14-15 -0.37277433013933603 2215 16-17 -0.3801840872892086 2215 18-19 -0.424278811836615 2215 20-21 -0.41831191284363456 2215 22-23 -0.4072170825568122 2215 24-25 -0.36893370198878017 2215 26-27 -0.3976477861742893 2215 28-29 -0.36073312795241463 2215 30-31 -0.36521729229988864 2215 32-33 -0.3218213730565225 2215 34-35 -0.392967419893651 2215 36-37 -0.42487048503163294 2215 38-39 -0.41618112277581787 2215 40-41 -0.40958760728643995 2215 42-43 -0.3618765580890084 2215 44-45 -0.4126385102517105 2215 46-47 -0.43071533898886116 2215 48-49 -0.36706320318356944 2215 50-51 -0.3515327831943722 2215 52-53 -0.3494691521925688 2215 54-55 -0.31664963463239104 2215 56-57 -0.40373620699617163 2215 58-59 -0.31929098703246694 2215 60-61 -0.3889878378309817 2215 62-63 -0.3933955999950669 2215 64-65 -0.3792907842953852 2215 66-67 -0.31264444693614024 2215 68-69 -0.3663227383671739 2215 70-71 -0.3705849718814207 2215 72-73 -0.3564297913157617 2215 74-75 -0.3669000857152014 2215 76-77 -0.23718830888564923 2215 78-79 -0.3033066696469646 2215 80-81 -0.32375684201349486 2215 82-83 -0.34291003820532495 2215 84-85 -0.3978811298942446 2215 86-87 -0.3587708674628942 2215 88-89 -0.39176253227603297 2215 90-91 -0.39494962490679697 2215 92-93 -0.3753253323694743 2215 94-95 -0.3816819013208317 2215 96-97 -0.37482809513239346 2215 98-99 -0.3197469411098446 2215 100 -0.25247044096780513 2216 1 -0.39858217225743076 2216 2 -0.39818755900763136 2216 3 -0.4289263429922876 2216 4 -0.22837279265262822 2216 5 -0.4543206830649069 2216 6 -0.3754632755274798 2216 7 -0.5584031969861911 2216 8 -0.2897380091174071 2216 9 -0.5360688365731292 2216 10-11 -0.39499822405728935 2216 12-13 -0.3445670278482993 2216 14-15 -0.6736469452120701 2216 16-17 -0.4142030772725178 2216 18-19 -0.3981627553138338 2216 20-21 -0.42568027976469835 2216 22-23 -0.4940402700120927 2216 24-25 -0.4275073366179285 2216 26-27 -0.5262986179176679 2216 28-29 -0.5811634135474577 2216 30-31 -0.6636890607234562 2216 32-33 -0.6185722674042466 2216 34-35 -0.59206339831006 2216 36-37 -0.6408122182775173 2216 38-39 -0.5927876395895524 2216 40-41 -0.5110535773555966 2216 42-43 -0.5560751181891774 2216 44-45 -0.7447310156177949 2216 46-47 -0.6791053938421854 2216 48-49 -0.7941627888024598 2216 50-51 -0.6013314058988897 2216 52-53 -0.5437730515486408 2216 54-55 -0.5200763577423331 2216 56-57 -0.62791884770688 2216 58-59 -0.6375755237603542 2216 60-61 -0.5080084972633614 2216 62-63 -0.5076498332409471 2216 64-65 -0.5454109250060952 2216 66-67 -0.42776096553856746 2216 68-69 -0.38644356474447505 2216 70-71 -0.4952105532074498 2216 72-73 -0.38792631826543555 2216 74-75 -0.3604220121401909 2216 76-77 -0.3234456127253793 2216 78-79 -0.5417304115978325 2216 80-81 -0.7361236885434224 2216 82-83 -0.5855101455270528 2216 84-85 -0.7016282530757323 2216 86-87 -0.53602378899879 2216 88-89 -0.8685298379044646 2216 90-91 -0.6547099248113994 2216 92-93 -0.7380282449618889 2216 94-95 -0.7500633257186387 2216 96-97 -0.8039605778416714 2216 98-99 -0.8692064420638168 2216 100 -0.7772295335029824 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 301.0 3 45.0 4 39.0 5 84.0 6 244.0 7 706.0 8 1658.0 9 2837.0 10 3813.0 11 5276.0 12 7386.0 13 10567.0 14 12428.0 15 16316.0 16 20491.0 17 28298.0 18 28314.0 19 15987.0 20 12365.0 21 12182.0 22 12944.0 23 14407.0 24 16891.0 25 21278.0 26 27368.0 27 36100.0 28 47540.0 29 62947.0 30 82286.0 31 107152.0 32 137943.0 33 180906.0 34 246354.0 35 355533.0 36 575925.0 37 1038112.0 38 1463031.0 39 586458.0 40 1966.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.6711168079767 20.508273218712585 14.683540549758229 23.13706942355248 2 15.387263166770559 20.559043661365013 40.119834947804186 23.93385822406024 3 17.910962371965 25.4519125887144 30.902797162679292 25.73432787664131 4 12.56752607337604 17.290274763089627 38.238513035484814 31.903686128049515 5 12.666951327929391 37.94148324432214 35.70202819224569 13.689537235502778 6 30.657119090141315 38.24102022205508 17.243930428414643 13.857930259388962 7 26.486607508973954 33.1950390395339 22.41674716882043 17.901606282671715 8 24.91638657557185 36.583320515143015 20.125476446839997 18.37481646244514 9 24.814331680680908 16.649699931350177 21.624251753496694 36.91171663447223 10-11 23.80589541432267 27.18148965112568 28.682882091328523 20.32973284322313 12-13 24.310949190907753 24.661633992611513 29.21079115789348 21.816625658587252 14-15 21.57529743633281 26.596586410209465 26.832924148416982 24.995192005040742 16-17 20.700501571091458 30.07226135138122 27.53647238471315 21.690764692814177 18-19 20.592662224961455 28.48836644077608 30.362453271277673 20.55651806298479 20-21 21.5456209197637 27.606184686018832 30.304869086397655 20.543325307819817 22-23 21.249238133263823 27.35379762894366 30.407367208023594 20.98959702976892 24-25 21.11170297962911 27.5237694995757 30.306265968899243 21.05826155189594 26-27 20.8824303990858 27.56841311301707 30.369429270193386 21.179727217703746 28-29 20.987814035319747 27.57292649986009 30.477107387207464 20.962152077612693 30-31 21.20136036768276 27.771808832379307 30.155311082268515 20.87151971766942 32-33 20.937770540164934 27.63974527521485 30.450311668045376 20.97217251657484 34-35 21.09190406386476 27.460216635882368 30.38886594250643 21.059013357746444 36-37 21.30479428715978 27.666564506820134 29.95490977527272 21.073731430747365 38-39 21.162066446919816 27.838590406475205 30.01004723877083 20.98929590783415 40-41 21.129825983292818 27.678190615120208 30.04294468783885 21.149038713748123 42-43 21.115523084152684 27.698519054275415 29.916145132565504 21.269812729006397 44-45 21.259952418593173 27.828635320743267 29.72068736228132 21.19072489838224 46-47 21.351358141315846 27.926859917675923 29.376536068498222 21.345245872510006 48-49 21.38878985129473 27.9950508582645 29.1217949359661 21.494364354474673 50-51 21.485986356029038 28.036076158511708 28.93639188939052 21.54154559606873 52-53 21.41976258135361 28.14682315924314 28.936396190320714 21.49701806908254 54-55 21.492145420110123 28.324355969304566 28.785732705086296 21.39776590549901 56-57 21.42241101328243 28.13709041660771 28.826662277370772 21.613836292739084 58-59 21.327303716519324 27.87574787459087 29.07317502332528 21.723773385564517 60-61 21.45523097410851 28.137475111741484 28.878850430770814 21.528443483379192 62-63 21.31651943047088 28.170387847062763 28.924349084206806 21.588743638259547 64-65 21.31836058384521 27.973827495798886 29.03458281218506 21.67322910817084 66-67 21.262247664965127 28.035174746075516 28.917171223634032 21.78540636532533 68-69 21.503560703098206 28.101009364009517 28.82112009074371 21.574309842148566 70-71 21.56786891261139 27.98517882085303 28.795051495743014 21.65190077079257 72-73 21.369054981569175 28.136823679629448 28.823733533419738 21.670387805381637 74-75 21.46665967198471 28.193403597801296 28.660202361282707 21.679734368931292 76-77 21.53580278000933 28.146617452535335 28.550491361633963 21.767088405821376 78-79 21.508340093941328 28.205307889061597 28.633661551706812 21.652690465290263 80-81 21.474108517461175 28.121096121114604 28.62800237103639 21.77679299038783 82-83 21.394889042151423 28.21201181458399 28.697888265838728 21.695210877425858 84-85 21.464484089244696 28.107974282816635 28.54477597573362 21.882765652205048 86-87 21.485781258783476 28.224792334531802 28.526886200016477 21.762540206668245 88-89 21.366784130257816 28.093560043935984 28.61848358864314 21.921172237163063 90-91 21.312695524945376 28.069046175171096 28.627826388287275 21.990431911596254 92-93 21.356920204872267 28.232225403772187 28.510719851161603 21.900134540193942 94-95 21.301363909434126 28.031611210488272 28.590531942102267 22.076492937975335 96-97 21.30941831459915 28.135101746382645 28.537347499786787 22.018132439231415 98-99 21.24023336917359 28.184525671667938 28.4825035687849 22.09273739037357 100 21.354822016839595 28.019584077079575 28.46515802329575 22.16043588278508 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 599.0 1 439.0 2 524.5 3 804.0 4 537.5 5 339.0 6 934.5 7 2632.5 8 4493.5 9 5104.5 10 3915.0 11 2509.5 12 2400.0 13 2806.0 14 3212.5 15 3666.5 16 4211.5 17 4824.5 18 5713.5 19 6727.5 20 8008.0 21 10001.0 22 13031.5 23 17556.5 24 23503.0 25 30997.0 26 40902.0 27 52681.5 28 66337.5 29 82077.5 30 97466.5 31 111065.5 32 122531.5 33 133017.0 34 144408.0 35 154307.5 36 162518.5 37 171314.5 38 178928.5 39 183105.0 40 186036.0 41 188659.0 42 189156.0 43 188256.0 44 185247.5 45 181357.5 46 177546.0 47 171082.5 48 164085.5 49 159194.0 50 154649.0 51 147864.5 52 137835.5 53 129532.5 54 123275.0 55 116057.0 56 107210.0 57 96947.5 58 87565.0 59 79735.5 60 70587.0 61 59069.0 62 49629.0 63 41583.0 64 33522.0 65 26986.0 66 21439.5 67 16377.5 68 12424.5 69 9551.5 70 6963.5 71 4847.5 72 3406.5 73 2383.5 74 1699.5 75 1133.5 76 694.0 77 407.5 78 245.5 79 159.5 80 101.0 81 49.5 82 25.0 83 13.5 84 8.0 85 6.5 86 4.5 87 5.0 88 3.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03386288285367654 2 0.0 3 0.0 4 5.775363761286504E-5 5 0.0 6 1.9251212537621684E-5 7 0.0 8 7.122948638920022E-4 9 0.0 10-11 0.0 12-13 0.042516302889337484 14-15 9.625606268810842E-5 16-17 0.0 18-19 2.887681880643252E-5 20-21 4.812803134405421E-5 22-23 0.0 24-25 1.1550727522573008E-4 26-27 1.1550727522573008E-4 28-29 3.561474319460011E-4 30-31 0.0 32-33 1.7326091283859514E-4 34-35 2.887681880643252E-5 36-37 7.700485015048673E-5 38-39 3.0801940060194694E-4 40-41 1.0588166895691926E-4 42-43 5.197827385157854E-4 44-45 2.3101455045146016E-4 46-47 1.4438409403216262E-4 48-49 8.085509265801106E-4 50-51 4.3315228209648784E-4 52-53 4.812803134405421E-4 54-55 4.7165470717173123E-4 56-57 4.427778883652987E-4 58-59 1.4438409403216262E-4 60-61 2.0213773164502765E-4 62-63 8.951813829994082E-4 64-65 3.6577303821481197E-4 66-67 0.0013379592713647069 68-69 0.0013187080588270854 70-71 3.753986444836228E-4 72-73 0.0010684422958380034 74-75 0.0010684422958380034 76-77 6.73792438816759E-4 78-79 0.003311208556470929 80-81 0.0024064015672027104 82-83 9.72186233149895E-4 84-85 0.0025026576298908185 86-87 0.001270580027483031 88-89 0.0019732492851062223 90-91 0.0024641552048155755 92-93 0.0023005198982457907 94-95 0.0034940950755783353 96-97 0.002714420967804657 98-99 0.0025604112675036836 100 0.0010395654770315708 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5194478.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.16127655686447 #Duplication Level Percentage of deduplicated Percentage of total 1 75.94531429840325 45.689670567042306 2 14.24951944852337 17.145385606900668 3 4.431472482294606 7.998091246843815 4 1.8664980648862435 4.491636250978946 5 0.9986445764580373 3.003986627315238 6 0.5914843360622262 2.1350671632535767 7 0.3787861380542284 1.5951780325170954 8 0.2717800888155574 1.3080509668705549 9 0.20172692004587017 1.0922534123259673 >10 0.9915994589444966 10.564066614409327 >50 0.055061244616721496 2.2578450743497687 >100 0.017408319311030963 1.7148330798142197 >500 4.483969713250297E-4 0.21490340171687639 >1k 1.6014163321638938E-4 0.16596088389230992 >5k 3.2028326639369455E-5 0.13848378874337944 >10k+ 6.405665327873891E-5 0.4845872830259148 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13061 0.25144008695387676 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12088 0.23270865715477088 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7187 0.13835846450788702 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.9251212537621684E-5 0.0 6 0.0 0.0 0.0 1.9251212537621684E-5 0.0 7 0.0 0.0 0.0 1.9251212537621684E-5 0.0 8 0.0 0.0 0.0 1.9251212537621684E-5 0.0 9 0.0 0.0 0.0 1.9251212537621684E-5 0.0 10-11 0.0 0.0 0.0 2.8876818806432527E-5 0.0 12-13 0.0 1.9251212537621684E-5 0.0 3.850242507524337E-5 0.0 14-15 0.0 6.737924388167589E-5 0.0 3.850242507524337E-5 0.0 16-17 0.0 1.155072752257301E-4 0.0 4.812803134405421E-5 0.0 18-19 0.0 1.3475848776335178E-4 0.0 5.775363761286505E-5 0.0 20-21 0.0 1.5400970030097347E-4 0.0 6.737924388167589E-5 0.0 22-23 0.0 1.5400970030097347E-4 0.0 1.636353065697843E-4 0.0 24-25 0.0 1.5400970030097347E-4 0.0 3.0801940060194694E-4 0.0 26-27 0.0 1.5400970030097347E-4 0.0 6.545412262791372E-4 0.0 28-29 0.0 1.636353065697843E-4 0.0 0.0024545295985467642 0.0 30-31 0.0 1.7326091283859514E-4 0.0 0.007142199851457644 0.0 32-33 0.0 1.7326091283859514E-4 0.0 0.01762448507819265 0.0 34-35 0.0 1.7326091283859514E-4 0.0 0.03402651816024632 0.0 36-37 0.0 2.2138894418264934E-4 0.0 0.05974613811050889 0.0 38-39 0.0 3.080194006019469E-4 0.0 0.09332987838238992 0.0 40-41 0.0 3.6577303821481197E-4 0.0 0.12588367878350817 0.0 42-43 0.0 4.042754632900553E-4 0.0 0.1604492308948079 0.0 44-45 0.0 4.042754632900553E-4 0.0 0.2015794464814366 0.0 46-47 0.0 4.042754632900553E-4 0.0 0.25190211605477975 0.0 48-49 0.0 4.042754632900553E-4 0.0 0.3050065858398091 0.0 50-51 0.0 5.005315259781637E-4 0.0 0.35636882089018374 0.0 52-53 0.0 5.005315259781637E-4 0.0 0.4052283983106676 0.0 54-55 0.0 5.005315259781637E-4 0.0 0.46010397964915817 0.0 56-57 0.0 5.005315259781637E-4 0.0 0.5275217259559093 0.0 58-59 0.0 5.005315259781637E-4 0.0 0.5987030843137655 0.0 60-61 0.0 5.197827385157854E-4 0.0 0.6735614242663074 0.0 62-63 0.0 5.197827385157854E-4 0.0 0.7451855605125288 0.0 64-65 0.0 5.197827385157854E-4 0.0 0.8227390702203379 0.0 66-67 0.0 5.197827385157854E-4 0.0 0.9079353113055826 0.0 68-69 0.0 5.197827385157854E-4 0.0 1.0051443090143033 0.0 70-71 0.0 5.197827385157854E-4 0.0 1.1053468702726241 0.0 72-73 0.0 5.294083447845963E-4 0.0 1.2052414121303432 0.0 74-75 0.0 5.390339510534071E-4 0.0 1.3107765592615852 0.0 76-77 0.0 5.390339510534071E-4 0.0 1.4225490992550165 0.0 78-79 0.0 5.390339510534071E-4 0.0 1.5478841184812024 0.0 80-81 0.0 5.390339510534071E-4 0.0 1.6877923055983681 0.0 82-83 0.0 5.390339510534071E-4 0.0 1.8262759029877498 0.0 84-85 0.0 5.390339510534071E-4 0.0 1.9658183170667005 0.0 86-87 0.0 5.582851635910288E-4 0.0 2.1140237767875814 0.0 88 0.0 5.775363761286505E-4 0.0 2.231735315848869 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8505 0.0 55.720936 1 GTATCAA 15525 0.0 41.639282 1 ATCAACG 18955 0.0 33.570335 3 TCAACGC 18985 0.0 33.542038 4 CAACGCA 19620 0.0 32.432503 5 TATCAAC 19965 0.0 32.03683 2 AACGCAG 20305 0.0 31.386896 6 ACGCAGA 23395 0.0 27.020182 7 GTGGTAT 3855 0.0 26.952562 1 CGCAGAG 23720 0.0 26.511162 8 GTACATG 14435 0.0 24.199379 1 GCAGAGT 25755 0.0 23.996387 9 TGGTATC 4130 0.0 23.554888 2 TACATGG 14780 0.0 22.607676 2 GAGTACT 17865 0.0 21.724533 12-13 ACATGGG 15230 0.0 21.29168 3 CAGAGTA 25030 0.0 21.036589 10-11 AGAGTAC 23800 0.0 20.91828 10-11 AGTACTT 18780 0.0 19.664684 12-13 GTACTTT 19720 0.0 19.613438 14-15 >>END_MODULE