FastQCFastQC Report
Wed 25 May 2016
SRR2049350_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049350_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6103281
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64000.046.2759781
GTATCAA106700.035.5112271
TCAACGC113850.031.7514384
ATCAACG114850.031.4340523
CAACGCA120750.029.8981425
TATCAAC129800.028.2843652
AACGCAG129750.027.8935416
ACGCAGA145700.024.2589197
CGCAGAG143600.024.220718
GTACATG155150.022.5735341
TACATGG154250.021.0887582
GCAGAGT163950.020.0963069
ACATGGG159400.019.6406573
GTGGTAT40100.019.2262461
TGGTATC37150.018.34762
CAGAGTA157350.017.83270510-11
AGAGTAC140050.017.50172410-11
CATGGGG106600.016.93344
GAGTACA121250.016.90441
GAGTACT71650.015.6123612-13
Produced by FastQC (version 0.11.5)