##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049350_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6103281
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.691909155092155	31.0	31.0	34.0	28.0	34.0
2	30.966640893643927	31.0	31.0	34.0	28.0	34.0
3	31.033342394033635	31.0	31.0	34.0	28.0	34.0
4	34.63412892180452	37.0	35.0	37.0	32.0	37.0
5	34.60111897191035	37.0	35.0	37.0	32.0	37.0
6	34.66263883311288	37.0	35.0	37.0	32.0	37.0
7	34.60476848436112	37.0	35.0	37.0	32.0	37.0
8	34.57694689135237	37.0	35.0	37.0	32.0	37.0
9	36.132774158686125	39.0	37.0	39.0	32.0	39.0
10-11	36.097797889364756	39.0	37.0	39.0	32.0	39.0
12-13	36.02771378214439	39.0	37.0	39.0	31.5	39.0
14-15	37.229304778855834	40.0	38.0	41.0	31.0	41.0
16-17	37.20275913889594	40.0	38.0	41.0	31.0	41.0
18-19	37.24754291994748	40.0	38.0	41.0	31.0	41.0
20-21	37.31946136840168	40.0	38.0	41.0	31.5	41.0
22-23	37.274534631454785	40.0	38.0	41.0	31.0	41.0
24-25	37.283933592439865	40.0	38.0	41.0	31.0	41.0
26-27	37.26605558551212	40.0	38.0	41.0	31.0	41.0
28-29	37.1999889076056	40.0	37.0	41.0	31.0	41.0
30-31	37.13209001846712	40.0	37.0	41.0	31.0	41.0
32-33	37.063244507339576	40.0	37.0	41.0	31.0	41.0
34-35	36.94942752922567	40.0	37.0	41.0	30.0	41.0
36-37	36.87783300490343	40.0	37.0	41.0	30.0	41.0
38-39	36.77410469876776	40.0	37.0	41.0	30.0	41.0
40-41	36.67212397725092	40.0	37.0	41.0	30.0	41.0
42-43	36.55113413588527	40.0	37.0	41.0	30.0	41.0
44-45	36.45646513735809	40.0	36.5	41.0	30.0	41.0
46-47	36.349909417573926	40.0	36.0	41.0	30.0	41.0
48-49	36.23022199043432	40.0	36.0	41.0	29.5	41.0
50-51	36.10466067349677	40.0	36.0	41.0	29.0	41.0
52-53	35.97512796805522	39.5	35.5	41.0	29.0	41.0
54-55	35.84943811369655	39.0	35.0	41.0	28.0	41.0
56-57	35.73502293274716	39.0	35.0	41.0	28.0	41.0
58-59	35.53984741977307	39.0	35.0	41.0	28.0	41.0
60-61	35.33357345008365	39.0	35.0	41.0	28.0	41.0
62-63	35.13204315842577	39.0	35.0	40.0	27.0	41.0
64-65	34.88416730279992	38.0	34.0	40.0	26.5	41.0
66-67	34.612980952376276	38.0	34.0	40.0	26.0	41.0
68-69	34.30266327242675	37.0	34.0	40.0	26.0	41.0
70-71	33.981646429191116	37.0	34.0	40.0	26.0	41.0
72-73	33.59690083088096	36.0	33.5	39.0	25.0	41.0
74-75	33.20391884299609	36.0	33.0	39.0	24.0	40.5
76-77	32.805364442502324	35.0	33.0	38.5	22.5	40.0
78-79	32.3993439758058	35.0	33.0	37.5	21.5	39.5
80-81	31.93172614860761	35.0	32.5	37.0	19.5	39.0
82-83	31.504112378243768	35.0	32.0	36.5	17.5	39.0
84-85	31.105963333492262	35.0	32.0	36.0	10.0	37.5
86-87	30.681380883495287	35.0	32.0	36.0	4.5	37.0
88-89	30.301672821552867	35.0	31.0	35.0	2.0	37.0
90-91	29.958969855721868	35.0	31.0	35.0	2.0	36.0
92-93	29.59533978527287	35.0	31.0	35.0	2.0	36.0
94-95	29.28393219974633	35.0	31.0	35.0	2.0	36.0
96-97	28.924457844887037	34.0	30.5	35.0	2.0	36.0
98-99	28.604024376396893	34.0	30.0	35.0	2.0	35.0
100	28.397722634759894	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3226676127395045
1101	2	0.4317637124411604
1101	3	0.373172203256928
1101	4	0.43302215410525946
1101	5	0.41910951848908695
1101	6	0.5465523658156357
1101	7	0.5157779905296209
1101	8	0.5205822684518466
1101	9	0.6503706220867898
1101	10-11	0.6236753173495089
1101	12-13	0.665816285857808
1101	14-15	0.7571008608748073
1101	16-17	0.7718221107735417
1101	18-19	0.7395277225813572
1101	20-21	0.7017011317618369
1101	22-23	0.6794258881524442
1101	24-25	0.7371318428386431
1101	26-27	0.6880900871647597
1101	28-29	0.6892799724849255
1101	30-31	0.6923727837108373
1101	32-33	0.6556091635363117
1101	34-35	0.6926865409681398
1101	36-37	0.7267090136702308
1101	38-39	0.7417686388806857
1101	40-41	0.7460327067039287
1101	42-43	0.6988020796170744
1101	44-45	0.7010517209654381
1101	46-47	0.6224134811466726
1101	48-49	0.650303875716773
1101	50-51	0.6741930593993999
1101	52-53	0.6748285750661012
1101	54-55	0.6772311225785543
1101	56-57	0.6311953479771972
1101	58-59	0.6759543858178532
1101	60-61	0.6646763997522527
1101	62-63	0.6960232140837448
1101	64-65	0.6732035155565512
1101	66-67	0.6411002464165563
1101	68-69	0.5955765283074754
1101	70-71	0.6617527204957625
1101	72-73	0.70568489659005
1101	74-75	0.6609783577263002
1101	76-77	0.6865016654099207
1101	78-79	0.620733987454436
1101	80-81	0.6274489551445903
1101	82-83	0.6564093048956892
1101	84-85	0.5690987627123931
1101	86-87	0.6374139685260438
1101	88-89	0.7258378368691822
1101	90-91	0.6804385923434424
1101	92-93	0.6926026317698586
1101	94-95	0.6756835001596961
1101	96-97	0.6727825967344963
1101	98-99	0.7279558377478068
1101	100	0.7751965285821605
1102	1	0.28125864476185214
1102	2	0.35911986281535846
1102	3	0.3769497994083508
1102	4	0.4076798127797119
1102	5	0.3031019069795775
1102	6	0.46400954431541663
1102	7	0.535073192452991
1102	8	0.5132453844306681
1102	9	0.6387640997831596
1102	10-11	0.5545369551137753
1102	12-13	0.581742530793143
1102	14-15	0.6717002142681139
1102	16-17	0.687705456261952
1102	18-19	0.6960626443706133
1102	20-21	0.655761044528262
1102	22-23	0.6758192138671362
1102	24-25	0.7144420046605902
1102	26-27	0.6711495514644241
1102	28-29	0.7102056841612949
1102	30-31	0.6840772128205828
1102	32-33	0.714071776215313
1102	34-35	0.7486158284133637
1102	36-37	0.6772162827319974
1102	38-39	0.6956485839371709
1102	40-41	0.718327127894753
1102	42-43	0.7166911346005023
1102	44-45	0.6831206150323652
1102	46-47	0.6503440267839196
1102	48-49	0.6440579722328508
1102	50-51	0.6936708895749959
1102	52-53	0.6885842546767478
1102	54-55	0.6598252220767407
1102	56-57	0.6377965129553331
1102	58-59	0.7529428578750341
1102	60-61	0.7606525006616494
1102	62-63	0.7079486275492783
1102	64-65	0.7077687777675123
1102	66-67	0.8041563989882192
1102	68-69	0.8284799553965883
1102	70-71	0.7963814963230078
1102	72-73	0.713054239227283
1102	74-75	0.8229773689824924
1102	76-77	0.8463130062116377
1102	78-79	0.7997575787850124
1102	80-81	0.7386409850917666
1102	82-83	0.754406842700412
1102	84-85	0.8078407139247759
1102	86-87	0.7871521366486149
1102	88-89	0.7409778325916072
1102	90-91	0.8213250068033524
1102	92-93	0.8075255617557744
1102	94-95	0.8497092124458341
1102	96-97	0.8358270716826581
1102	98-99	0.9121172526477856
1102	100	0.9010730837913776
1103	1	0.22443076043557753
1103	2	0.301738969763921
1103	3	0.2562205942317881
1103	4	0.2871078060370138
1103	5	0.3224885863137743
1103	6	0.3991721265799484
1103	7	0.43190545845109085
1103	8	0.4500567083490594
1103	9	0.5359114884541043
1103	10-11	0.5509873914705565
1103	12-13	0.5376231038603834
1103	14-15	0.5668882851651915
1103	16-17	0.5443127122644853
1103	18-19	0.5336251094874598
1103	20-21	0.5558373033660331
1103	22-23	0.5381187060938757
1103	24-25	0.5236041809672471
1103	26-27	0.523905637559487
1103	28-29	0.5126501643147492
1103	30-31	0.534213368383682
1103	32-33	0.5577225480419585
1103	34-35	0.5305229199895365
1103	36-37	0.4443658937360624
1103	38-39	0.4577237626673707
1103	40-41	0.5163234908168164
1103	42-43	0.4683214024598428
1103	44-45	0.39309373614064924
1103	46-47	0.3951741305948602
1103	48-49	0.4471769444555491
1103	50-51	0.3435004499907137
1103	52-53	0.4422933037246821
1103	54-55	0.4098985420884915
1103	56-57	0.381098071179494
1103	58-59	0.5042626043921103
1103	60-61	0.5342103888787904
1103	62-63	0.5151683644747678
1103	64-65	0.5071464163087853
1103	66-67	0.5647166854845906
1103	68-69	0.5991601632496497
1103	70-71	0.49681995519716793
1103	72-73	0.4612372408822836
1103	74-75	0.4519651682805659
1103	76-77	0.5382550944674662
1103	78-79	0.4784483553466501
1103	80-81	0.4528758832372155
1103	82-83	0.3986006632887289
1103	84-85	0.47143837297593194
1103	86-87	0.46671699484080875
1103	88-89	0.4743931787083504
1103	90-91	0.5367258629466427
1103	92-93	0.4892527782810845
1103	94-95	0.5263567791170587
1103	96-97	0.44668434120159617
1103	98-99	0.495254367533537
1103	100	0.46399370497757886
1104	1	0.18869706225813587
1104	2	0.24223091911461836
1104	3	0.2573477762516312
1104	4	0.3512149907458664
1104	5	0.32827810245061784
1104	6	0.40244673860821933
1104	7	0.4083702514761427
1104	8	0.46584908641436584
1104	9	0.5166302937832725
1104	10-11	0.49703860321614
1104	12-13	0.44099405358834076
1104	14-15	0.5266283989703382
1104	16-17	0.5678770665933897
1104	18-19	0.5474351244975679
1104	20-21	0.4986078199322179
1104	22-23	0.5600671878092456
1104	24-25	0.6179481537578084
1104	26-27	0.5394745763063966
1104	28-29	0.5747390565480686
1104	30-31	0.4913974262099572
1104	32-33	0.5321582089338719
1104	34-35	0.5589633794263875
1104	36-37	0.5510022323997319
1104	38-39	0.5759502385912683
1104	40-41	0.595144536780225
1104	42-43	0.5416079586906548
1104	44-45	0.47517428332604794
1104	46-47	0.40055294342986514
1104	48-49	0.3893215112412278
1104	50-51	0.4624309406442251
1104	52-53	0.44524319843957016
1104	54-55	0.46725626352541383
1104	56-57	0.45042290404686014
1104	58-59	0.5782891306586109
1104	60-61	0.4821401424096905
1104	62-63	0.4528977478084002
1104	64-65	0.5253486023396263
1104	66-67	0.5465945317198191
1104	68-69	0.6072516796767218
1104	70-71	0.6044027286346818
1104	72-73	0.6614480272463084
1104	74-75	0.7011014837764904
1104	76-77	0.7150811024184236
1104	78-79	0.641165329051951
1104	80-81	0.6432513467150862
1104	82-83	0.5780104573277285
1104	84-85	0.6294895956453033
1104	86-87	0.6775320791090209
1104	88-89	0.6487436224185714
1104	90-91	0.6778907757761239
1104	92-93	0.655767450298022
1104	94-95	0.6639681027242865
1104	96-97	0.6360239928839917
1104	98-99	0.6044593361789019
1104	100	0.5724607420900298
1105	1	0.15076332202176346
1105	2	0.22051770907904
1105	3	0.25369919530041685
1105	4	0.25400299221807643
1105	5	0.21232096017768498
1105	6	0.24598779185321717
1105	7	0.2696289671756489
1105	8	0.28074334785468125
1105	9	0.3679950291940628
1105	10-11	0.38786552118121165
1105	12-13	0.33187496543964556
1105	14-15	0.35726746932979836
1105	16-17	0.37045497114498005
1105	18-19	0.30643676795645547
1105	20-21	0.3258743490901921
1105	22-23	0.3221860995992074
1105	24-25	0.31562264666256823
1105	26-27	0.36971971493969136
1105	28-29	0.4043391439606552
1105	30-31	0.38528090932042147
1105	32-33	0.3796491226254446
1105	34-35	0.39897949452473824
1105	36-37	0.3439228990868486
1105	38-39	0.3621638422390845
1105	40-41	0.416282600105788
1105	42-43	0.43726461380455106
1105	44-45	0.42046953084169303
1105	46-47	0.41444502722326604
1105	48-49	0.4423562871378408
1105	50-51	0.39855872915687485
1105	52-53	0.3553063169730848
1105	54-55	0.39427058692131567
1105	56-57	0.3414798551367042
1105	58-59	0.36084105424671975
1105	60-61	0.40484837350608416
1105	62-63	0.3234894185645416
1105	64-65	0.37078295599766875
1105	66-67	0.4094353641330244
1105	68-69	0.4110219225917007
1105	70-71	0.45380739012815496
1105	72-73	0.4344337677485015
1105	74-75	0.4235061350462743
1105	76-77	0.4815450843429332
1105	78-79	0.42320383148519625
1105	80-81	0.45824337223342937
1105	82-83	0.47295432924528313
1105	84-85	0.41730352696252737
1105	86-87	0.47177032441634736
1105	88-89	0.4647420428577824
1105	90-91	0.4290578743938802
1105	92-93	0.46889045667760954
1105	94-95	0.43380750126434364
1105	96-97	0.47982958422247535
1105	98-99	0.5557808125826149
1105	100	0.5176579060371935
1106	1	0.2111955983036502
1106	2	0.23683193459559249
1106	3	0.23794743006802577
1106	4	0.26054940824538875
1106	5	0.258741138315969
1106	6	0.2940242208084669
1106	7	0.33236271047206856
1106	8	0.3041068995037648
1106	9	0.4064574874405409
1106	10-11	0.39986246485376853
1106	12-13	0.344754678606364
1106	14-15	0.43604590577841407
1106	16-17	0.4776631229111956
1106	18-19	0.46651117478478454
1106	20-21	0.40287760060071776
1106	22-23	0.4403624745409047
1106	24-25	0.42669424894868513
1106	26-27	0.3960095977581517
1106	28-29	0.4396234975980633
1106	30-31	0.44720579609725064
1106	32-33	0.4946520154670111
1106	34-35	0.5084824979291724
1106	36-37	0.4503442927563128
1106	38-39	0.49309258730148997
1106	40-41	0.5466246285042189
1106	42-43	0.5219204288641919
1106	44-45	0.4781022529787151
1106	46-47	0.45283527055968165
1106	48-49	0.49972886343377354
1106	50-51	0.4936796450287204
1106	52-53	0.521590026855371
1106	54-55	0.5265960843787454
1106	56-57	0.4799537375838483
1106	58-59	0.522581451031563
1106	60-61	0.5531258898867435
1106	62-63	0.5238133196526604
1106	64-65	0.580542647795184
1106	66-67	0.5785484150417446
1106	68-69	0.5995570344149073
1106	70-71	0.5798161701593685
1106	72-73	0.572664673673458
1106	74-75	0.5817243215247032
1106	76-77	0.6230406018299774
1106	78-79	0.4669211626107952
1106	80-81	0.5061453946659888
1106	82-83	0.3877768664102561
1106	84-85	0.44970383595366314
1106	86-87	0.5097747651380473
1106	88-89	0.4910909805863568
1106	90-91	0.5199887689229392
1106	92-93	0.4882100677000878
1106	94-95	0.5137739460946058
1106	96-97	0.4645564609233688
1106	98-99	0.47897029139378944
1106	100	0.4359825597452378
1107	1	0.18494780770638286
1107	2	0.19096272939932746
1107	3	0.1985230814993706
1107	4	0.2764460228436505
1107	5	0.19357772895440917
1107	6	0.23783549809854065
1107	7	0.253911088393977
1107	8	0.29882525098348367
1107	9	0.3142510001390306
1107	10-11	0.3240318977131409
1107	12-13	0.32012128122471495
1107	14-15	0.2719388801103477
1107	16-17	0.31609136641549185
1107	18-19	0.3136052107098166
1107	20-21	0.2922351860645591
1107	22-23	0.34855876565055866
1107	24-25	0.3432895098020836
1107	26-27	0.361979781576089
1107	28-29	0.4290836342694462
1107	30-31	0.36431644353739756
1107	32-33	0.34141339032289153
1107	34-35	0.3504683637249997
1107	36-37	0.36325891581758896
1107	38-39	0.39213652057192405
1107	40-41	0.4372391224618255
1107	42-43	0.4463934431643821
1107	44-45	0.3821723096687748
1107	46-47	0.3779646585366052
1107	48-49	0.39502780235758905
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2216	90-91	-3.3580066568549576
2216	92-93	-3.360854562015092
2216	94-95	-3.203808269213848
2216	96-97	-3.465482741231625
2216	98-99	-3.1244402160559446
2216	100	-2.7501683505071135
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	113196.0
3	16090.0
4	9955.0
5	8190.0
6	7790.0
7	8482.0
8	10222.0
9	11671.0
10	13053.0
11	14387.0
12	16206.0
13	17977.0
14	20227.0
15	20685.0
16	20421.0
17	21467.0
18	20595.0
19	19313.0
20	19841.0
21	21102.0
22	23903.0
23	28231.0
24	34439.0
25	42446.0
26	52281.0
27	62296.0
28	77618.0
29	99064.0
30	120837.0
31	140859.0
32	172772.0
33	223292.0
34	294088.0
35	407629.0
36	641140.0
37	1106126.0
38	1550690.0
39	612279.0
40	2421.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.81415108407634	21.40163836373379	15.153701066624278	22.630509485565586
2	14.90752405200721	20.60521665245624	41.42678581789609	23.060473477640457
3	17.908611530162876	25.90785625355265	31.02190498856267	25.1616272277218
4	12.313343908777126	17.528699741645777	39.85790025973107	30.300056089846034
5	12.67577352809852	38.09798405048036	36.14933775333779	13.076904668083323
6	29.73977268629463	38.8578912342931	18.189685976639204	13.212650102773068
7	25.743986958090275	33.2596890286049	23.523921778045278	17.472402235259544
8	23.737299564725095	37.265963521878156	21.211325015095955	17.785411898300797
9	24.002037713099764	17.62531681505049	22.725910049849556	35.646735422000184
10-11	22.98548510914258	27.775016364277544	29.686524612635605	19.552973913944268
12-13	23.423671947626566	25.39718248819699	29.66322314676105	21.51592241741539
14-15	20.959782591770317	27.754961715072945	26.870257410098237	24.4149982830585
16-17	20.29480090576092	31.097452889557143	27.639033408108627	20.968712796573314
18-19	20.23252541525703	30.044326263657474	29.61009491788287	20.11305340320263
20-21	21.42275217120467	29.205447227636366	29.120756866627108	20.25104373453186
22-23	21.03807050749613	29.129685259710218	29.131824259453293	20.70041997334036
24-25	20.931826613528106	29.2048564890935	29.062383500570753	20.80093339680764
26-27	20.683983830597267	29.136214089369666	29.1864504955047	20.993351584528366
28-29	20.68216107685058	29.148288225532177	29.318076584236515	20.851474113380732
30-31	20.97809314394645	29.478318471187247	28.87835549713647	20.665232887729832
32-33	20.63230402426173	29.236847296276107	29.268783995231047	20.862064684231115
34-35	20.872571927588567	28.909501443665576	29.268287086208204	20.94963954253765
36-37	21.03780207395859	29.197591193584167	28.831938352597874	20.932668379859372
38-39	20.833578159671355	29.152297716694253	29.118698118840463	20.895426004793922
40-41	20.788177606343407	28.849877261870244	29.342640899717942	21.019304232068407
42-43	20.547958003326396	28.829651883912955	29.37514315837178	21.24724695438887
44-45	20.626433461686382	28.955854358530736	29.293087398763912	21.12462478101897
46-47	20.62886704134506	29.123076041805042	29.14687416488379	21.10118275196611
48-49	20.501241249117914	28.943644675855452	29.181134521279724	21.37397955374691
50-51	20.544561778237576	28.842838796513075	29.10934956081573	21.50324986443362
52-53	20.39424066242462	28.825403433492227	29.23953355249706	21.540822351586094
54-55	20.54014713509751	29.053465728860917	29.021496940756613	21.38489019528496
56-57	20.515507392792543	28.81523066705915	28.986324005777085	21.682937934371225
58-59	20.434241313501957	28.46822353501905	29.17455568543663	21.922979466042364
60-61	20.63808166573381	28.684689498281934	28.809645757854636	21.867583078129623
62-63	20.53919603849951	28.629152571707994	28.834386697511334	21.99726469228116
64-65	20.592167316645046	28.370814645942747	28.849149018640546	22.18786901877166
66-67	20.50294873158569	28.402645147364463	28.67319616673908	22.421209954310765
68-69	20.785460268155816	28.477345609467235	28.550288148021647	22.1869059743553
70-71	20.649441241206166	28.229451766847376	28.588080784869955	22.533026207076503
72-73	20.445546509834593	28.203328933849253	28.775535022444583	22.575589533871575
74-75	20.561502501618058	28.14520677130735	28.729772639327923	22.56351808774667
76-77	20.63960249116637	28.221355462902853	28.54804283411067	22.590999211820108
78-79	20.725169420466294	28.174744828553766	28.63806563359026	22.462020117389685
80-81	20.70318319633434	27.948565104010026	28.708220742739805	22.640030956915833
82-83	20.541342253379046	27.994636041008853	28.88048088790501	22.583540817707096
84-85	20.674812614650985	27.834877173631124	28.739587938664872	22.750722273053018
86-87	20.94094003367934	27.873591924038653	28.623440339143695	22.562027703138305
88-89	20.94817219629986	27.695576331687192	28.654201971075526	22.70204950093742
90-91	21.102325759778463	27.52047180625563	28.589249077002705	22.7879533569632
92-93	21.266557582350202	27.550053500047078	28.455428195823696	22.727960721779024
94-95	21.25908039127181	27.267192715560235	28.54695383009683	22.926773063071128
96-97	21.40797711919212	27.208111505926073	28.447649714964783	22.93626165991702
98-99	21.555848403506246	27.13959917624635	28.336373501400313	22.968178918847094
100	21.674014353918817	27.060821875971303	28.31683483031504	22.94832893979484
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1678.0
1	1365.0
2	1205.0
3	1459.5
4	1462.0
5	1249.5
6	1054.5
7	956.5
8	935.5
9	935.0
10	882.5
11	822.5
12	821.5
13	926.5
14	1109.5
15	1261.5
16	1525.5
17	1993.5
18	2712.5
19	3894.5
20	5847.0
21	8706.0
22	12893.0
23	19181.0
24	27312.0
25	37466.0
26	50698.0
27	66236.5
28	84099.0
29	103936.5
30	122102.0
31	138075.0
32	152557.5
33	166488.5
34	181249.0
35	194629.0
36	205366.0
37	215797.5
38	225463.5
39	232024.5
40	235977.0
41	236157.0
42	232929.0
43	228946.0
44	223398.5
45	216962.5
46	210761.0
47	202865.0
48	194432.0
49	186615.5
50	178864.0
51	169888.0
52	157887.0
53	145890.0
54	136453.5
55	128542.5
56	117300.5
57	104012.5
58	91999.5
59	80246.0
60	68436.5
61	56423.0
62	46561.0
63	38250.0
64	30603.5
65	24155.5
66	18868.0
67	14753.5
68	11443.0
69	8850.5
70	6961.0
71	5515.0
72	4022.0
73	2682.0
74	2000.0
75	1465.5
76	1023.0
77	767.5
78	575.0
79	420.5
80	310.0
81	222.0
82	186.0
83	153.5
84	118.0
85	99.0
86	78.5
87	57.5
88	46.5
89	42.5
90	37.0
91	36.0
92	30.0
93	27.0
94	28.5
95	27.5
96	25.0
97	28.5
98	40.5
99	52.5
100	111.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01394331999460618
2	0.034850107671595
3	0.036128108799185224
4	0.038585803275320275
5	0.04150226738700053
6	0.04032257403845571
7	0.03824172604866137
8	0.037832110302638854
9	0.03807787975025236
10-11	0.03833184151278632
12-13	0.03934768856292214
14-15	0.03794680271152516
16-17	0.034612530538901946
18-19	0.0372013020537642
20-21	0.03657868611980999
22-23	0.037660071689309405
24-25	0.03480914609699275
26-27	0.0348664923014359
28-29	0.03532526193698111
30-31	0.03530068499221976
32-33	0.035218761843015256
34-35	0.0351450310087312
36-37	0.03280202894148246
38-39	0.033055990704016415
40-41	0.03408003006907269
42-43	0.03413737627351584
44-45	0.03294129829513011
46-47	0.03210568217324419
48-49	0.0322367592119714
50-51	0.03320345237258452
52-53	0.03372776052749333
54-55	0.033055990704016415
56-57	0.03167148948246033
58-59	0.03232687467609635
60-61	0.03188448967039204
62-63	0.03205652828372149
64-65	0.029426795194256995
66-67	0.030213257426620208
68-69	0.031179950587233326
70-71	0.03260541338339165
72-73	0.029623410752347795
74-75	0.029983872608847606
76-77	0.030835873360574417
78-79	0.031016104288824325
80-81	0.031098027438028822
82-83	0.029779064735836344
84-85	0.030860450305335768
86-87	0.03136837383040368
88-89	0.03181895115102844
90-91	0.03216302837768735
92-93	0.03087683493517667
94-95	0.03190087430023294
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6103281.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.98386292930007
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.79509159176861	51.33220660163605
2	13.598505345394923	17.94563825507786
3	4.138744403279319	8.192710302161714
4	1.7649426721295685	4.658309414234803
5	0.8706688970120048	2.8725048578622503
6	0.5008781303211718	1.982992433723778
7	0.30662653354362873	1.4162682211880462
8	0.1954721921275621	1.031840826546789
9	0.14295454837342222	0.8489423992492698
>10	0.6457591142918842	7.177085886298064
>50	0.030068895959313457	1.3374627515807198
>100	0.010138683841919082	1.0935380497060694
>500	7.449757751421687E-5	0.030296618616608352
>1k	7.449437907608924E-5	0.08020338211786338
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.553851936360131E-5	0.0
2	0.0	3.276925968180066E-5	0.0	6.553851936360131E-5	0.0
3	0.0	3.276925968180066E-5	0.0	6.553851936360131E-5	0.0
4	0.0	3.276925968180066E-5	0.0	6.553851936360131E-5	0.0
5	0.0	3.276925968180066E-5	0.0	8.192314920450164E-5	0.0
6	0.0	3.276925968180066E-5	0.0	8.192314920450164E-5	0.0
7	0.0	4.915388952270099E-5	0.0	1.4746166856810297E-4	0.0
8	0.0	4.915388952270099E-5	0.0	1.6384629840900328E-4	0.0
9	0.0	4.915388952270099E-5	0.0	1.8023092824990362E-4	0.0
10-11	0.0	4.915388952270099E-5	0.0	2.5396176253395506E-4	0.0
12-13	0.0	4.915388952270099E-5	0.0	3.2769259681800656E-4	0.0
14-15	0.0	5.734620444315115E-5	0.0	3.850388012611577E-4	0.0
16-17	0.0	6.553851936360131E-5	0.0	4.587696355452092E-4	0.0
18-19	0.0	6.553851936360131E-5	0.0	5.079235250679102E-4	0.0
20-21	0.0	6.553851936360131E-5	0.0	8.192314920450164E-4	0.0
22-23	0.0	8.192314920450164E-5	0.0	0.001744963078055885	0.0
24-25	0.0	8.192314920450164E-5	0.0	0.00399784968117968	0.0
26-27	0.0	8.192314920450164E-5	0.0	0.008773969279802125	1.638462984090033E-5
28-29	0.0	9.830777904540198E-5	0.0	0.027583524337155704	1.638462984090033E-5
30-31	0.0	9.830777904540198E-5	0.0	0.08317657338733052	1.638462984090033E-5
32-33	0.0	1.146924088863023E-4	0.0	0.20592202784043534	1.638462984090033E-5
34-35	0.0	1.3107703872720263E-4	0.0	0.3941486554526983	1.638462984090033E-5
36-37	1.638462984090033E-5	1.5565398348855314E-4	0.0	0.6886214152682795	1.638462984090033E-5
38-39	1.638462984090033E-5	1.8023092824990362E-4	0.0	1.0610522438668644	1.638462984090033E-5
40-41	1.638462984090033E-5	1.8023092824990362E-4	0.0	1.4297719538064855	1.638462984090033E-5
42-43	1.638462984090033E-5	1.8023092824990362E-4	0.0	1.7856051523762382	1.638462984090033E-5
44-45	1.638462984090033E-5	1.884232431703538E-4	0.0	2.210048988404761	1.638462984090033E-5
46-47	1.638462984090033E-5	1.9661555809080396E-4	0.0	2.7034639237485543	1.638462984090033E-5
48-49	1.638462984090033E-5	1.9661555809080396E-4	0.0	3.191398200410566	1.638462984090033E-5
50-51	1.638462984090033E-5	1.9661555809080396E-4	0.0	3.6574917654946577	1.638462984090033E-5
52-53	1.638462984090033E-5	2.1300018793170427E-4	0.0	4.091512417665188	1.638462984090033E-5
54-55	1.638462984090033E-5	2.1300018793170427E-4	0.0	4.554378210670621	1.638462984090033E-5
56-57	1.638462984090033E-5	2.1300018793170427E-4	0.0	5.078350480667694	1.638462984090033E-5
58-59	1.638462984090033E-5	2.293848177726046E-4	0.0	5.623671923347459	1.638462984090033E-5
60-61	1.638462984090033E-5	2.293848177726046E-4	0.0	6.1487747328035525	1.638462984090033E-5
62-63	1.638462984090033E-5	2.293848177726046E-4	0.0	6.648120904149752	1.638462984090033E-5
64-65	1.638462984090033E-5	2.293848177726046E-4	0.0	7.157002930063355	1.638462984090033E-5
66-67	1.638462984090033E-5	2.293848177726046E-4	0.0	7.71326111316192	1.638462984090033E-5
68-69	1.638462984090033E-5	2.293848177726046E-4	0.0	8.301215362687708	1.638462984090033E-5
70-71	1.638462984090033E-5	2.293848177726046E-4	0.0	8.889128650638895	1.638462984090033E-5
72-73	1.638462984090033E-5	2.293848177726046E-4	0.0	9.466449275397938	1.638462984090033E-5
74-75	1.638462984090033E-5	2.293848177726046E-4	0.0	10.026533269564354	1.638462984090033E-5
76-77	1.638462984090033E-5	2.293848177726046E-4	0.0	10.611145054602598	1.638462984090033E-5
78-79	1.638462984090033E-5	2.3757713269305478E-4	0.0	11.231327871025437	1.638462984090033E-5
80-81	1.638462984090033E-5	2.457694476135049E-4	0.0	11.867887124974256	1.638462984090033E-5
82-83	1.638462984090033E-5	2.5396176253395506E-4	0.0	12.492075983393194	1.638462984090033E-5
84-85	1.638462984090033E-5	2.6215407745440526E-4	0.0	13.08637108466741	1.638462984090033E-5
86-87	1.638462984090033E-5	2.6215407745440526E-4	0.0	13.69308573536103	1.638462984090033E-5
88	1.638462984090033E-5	2.785387072953056E-4	0.0	14.160989802042542	1.638462984090033E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6400	0.0	46.275978	1
GTATCAA	10670	0.0	35.511227	1
TCAACGC	11385	0.0	31.751438	4
ATCAACG	11485	0.0	31.434052	3
CAACGCA	12075	0.0	29.898142	5
TATCAAC	12980	0.0	28.284365	2
AACGCAG	12975	0.0	27.893541	6
ACGCAGA	14570	0.0	24.258919	7
CGCAGAG	14360	0.0	24.22071	8
GTACATG	15515	0.0	22.573534	1
TACATGG	15425	0.0	21.088758	2
GCAGAGT	16395	0.0	20.096306	9
ACATGGG	15940	0.0	19.640657	3
GTGGTAT	4010	0.0	19.226246	1
TGGTATC	3715	0.0	18.3476	2
CAGAGTA	15735	0.0	17.832705	10-11
AGAGTAC	14005	0.0	17.501724	10-11
CATGGGG	10660	0.0	16.9334	4
GAGTACA	12125	0.0	16.9044	1
GAGTACT	7165	0.0	15.61236	12-13
>>END_MODULE