FastQCFastQC Report
Wed 25 May 2016
SRR2049350_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049350_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6103281
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT174540.28597732924307434No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153290.25115999083116114No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153270.2511272215714793No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT85190.1395806616146299RNA PCR Primer, Index 46 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83000.13599242767947273No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105800.058.168671
GTATCAA194850.040.6086771
ATCAACG239900.032.4410173
TCAACGC241650.032.206084
TATCAAC251650.031.1503892
GTGGTAT45100.031.0652521
CAACGCA250900.031.05
AACGCAG260800.029.8830246
TGGTATC45700.028.7942772
ACGCAGA291150.026.4771827
CGCAGAG290800.026.2664418
GCAGAGT313350.023.8056229
GTACATG150600.021.9465121
GAGTACT226650.021.46939512-13
TAGACCG2659.076757E-1021.2813785
AGAGTAC291300.021.02212110-11
CAGAGTA310350.020.90624410-11
TACATGG149950.020.3405442
AGTACTT237400.019.69514812-13
ACATGGG151700.019.2694423
Produced by FastQC (version 0.11.5)