##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2049350_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6103281
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.798020277945582	31.0	31.0	34.0	30.0	34.0
2	31.925228250182155	33.0	31.0	34.0	30.0	34.0
3	31.89721315469499	33.0	31.0	34.0	30.0	34.0
4	35.633686700645114	37.0	35.0	37.0	33.0	37.0
5	35.738905680403704	37.0	35.0	37.0	33.0	37.0
6	35.79487672286431	37.0	35.0	37.0	35.0	37.0
7	35.73797077342498	37.0	35.0	37.0	33.0	37.0
8	35.73117786973924	37.0	35.0	37.0	33.0	37.0
9	37.43792527330791	39.0	37.0	39.0	34.0	39.0
10-11	37.415496353518705	39.0	37.0	39.0	34.0	39.0
12-13	37.370366037546034	39.0	37.0	39.0	34.0	39.0
14-15	38.63164034230113	40.0	38.0	41.0	34.0	41.0
16-17	38.65610808350459	40.0	38.0	41.0	34.0	41.0
18-19	38.73971196803818	40.0	38.0	41.0	34.0	41.0
20-21	38.78162049232208	40.0	38.0	41.0	34.0	41.0
22-23	38.747162714612024	40.0	38.0	41.0	34.0	41.0
24-25	38.72808461547158	40.0	38.0	41.0	34.0	41.0
26-27	38.60950118469066	40.0	38.0	41.0	34.0	41.0
28-29	38.52693788799827	40.0	38.0	41.0	34.0	41.0
30-31	38.4226852737077	40.0	38.0	41.0	34.0	41.0
32-33	38.27160972925874	40.0	38.0	41.0	34.0	41.0
34-35	38.16337548607053	40.0	38.0	41.0	34.0	41.0
36-37	38.03959075454661	40.0	38.0	41.0	33.0	41.0
38-39	37.932533501243014	40.0	37.5	41.0	33.0	41.0
40-41	37.83627576708331	40.0	37.0	41.0	33.0	41.0
42-43	37.71232309310353	40.0	37.0	41.0	33.0	41.0
44-45	37.53760387240895	40.0	37.0	41.0	33.0	41.0
46-47	37.30419613647152	40.0	37.0	41.0	32.0	41.0
48-49	37.09696120824193	40.0	37.0	41.0	31.0	41.0
50-51	36.94507806538811	40.0	37.0	41.0	31.0	41.0
52-53	36.820792537653105	40.0	36.0	41.0	31.0	41.0
54-55	36.695262187665946	40.0	36.0	41.0	31.0	41.0
56-57	36.56424806591733	39.0	36.0	41.0	31.0	41.0
58-59	36.38926693691475	39.0	35.5	41.0	30.0	41.0
60-61	36.219557398717185	39.0	35.0	41.0	30.0	41.0
62-63	36.02968182195774	39.0	35.0	41.0	30.0	41.0
64-65	35.79693487814177	38.5	35.0	40.5	29.5	41.0
66-67	35.50103272321887	38.0	35.0	40.0	29.0	41.0
68-69	35.1758650797825	37.5	35.0	40.0	29.0	41.0
70-71	34.81701964893965	37.0	34.0	40.0	28.0	41.0
72-73	34.514737319156694	36.5	34.0	39.0	28.0	41.0
74-75	34.19580312622014	36.0	34.0	39.0	28.0	41.0
76-77	33.845828006280556	35.5	34.0	39.0	28.0	40.0
78-79	33.483177900542344	35.0	34.0	37.5	27.0	39.5
80-81	33.13344412620032	35.0	34.0	37.0	26.5	39.0
82-83	32.80718911680455	35.0	33.0	36.5	26.0	39.0
84-85	32.510154128574456	35.0	33.0	36.0	26.0	38.0
86-87	32.24818536128355	35.0	33.0	36.0	26.0	37.0
88-89	31.989142888882224	35.0	33.0	35.5	25.5	37.0
90-91	31.76463020463911	35.0	33.0	35.0	25.0	36.0
92-93	31.559634973385627	35.0	33.0	35.0	25.0	36.0
94-95	31.382245713412182	35.0	33.0	35.0	24.5	36.0
96-97	31.16159275642069	35.0	33.0	35.0	23.5	36.0
98-99	30.982823664845185	35.0	33.0	35.0	23.0	35.0
100	30.850787633733397	35.0	32.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1574312405943843
1101	2	0.18412035815011762
1101	3	0.3847097210845547
1101	4	0.2209447981494037
1101	5	0.18383089265898178
1101	6	0.13795819577256196
1101	7	0.07436299007436986
1101	8	0.15765083068498598
1101	9	0.21263724161642017
1101	10-11	0.2517153483729473
1101	12-13	0.18466685495620538
1101	14-15	0.3082776621239418
1101	16-17	0.18066532833239535
1101	18-19	0.2712443007179317
1101	20-21	0.24018734982076495
1101	22-23	0.2099265649016928
1101	24-25	0.21264434589319592
1101	26-27	0.239391064923268
1101	28-29	0.2206385411229732
1101	30-31	0.17542311586152692
1101	32-33	0.26981015068296443
1101	34-35	0.28100239501647906
1101	36-37	0.23656012399055015
1101	38-39	0.24732212345522697
1101	40-41	0.23856201564283452
1101	42-43	0.25983813429147773
1101	44-45	0.3365787059830936
1101	46-47	0.32076750101898455
1101	48-49	0.33007077056213063
1101	50-51	0.22930885849217475
1101	52-53	0.2713752133851486
1101	54-55	0.2351718093116162
1101	56-57	0.23498072902931
1101	58-59	0.24240228629341232
1101	60-61	0.280986181027437
1101	62-63	0.26409196617107256
1101	64-65	0.18800938544983836
1101	66-67	0.14722040323952967
1101	68-69	0.1619003012669964
1101	70-71	0.14238630720713985
1101	72-73	0.12669093288324973
1101	74-75	0.15755085671675317
1101	76-77	0.1587656673456337
1101	78-79	0.18268902616857474
1101	80-81	0.2674393389141372
1101	82-83	0.2388919683203241
1101	84-85	0.24905857455741653
1101	86-87	0.19074067656467975
1101	88-89	0.24501781675148493
1101	90-91	0.24913622044318373
1101	92-93	0.28275437818613014
1101	94-95	0.3385743952267717
1101	96-97	0.4111289930284201
1101	98-99	0.4125038423545426
1101	100	0.432046467092718
1102	1	0.2351985468611737
1102	2	0.20914274409684452
1102	3	0.3919591955052084
1102	4	0.20392912010574094
1102	5	0.16589098536435642
1102	6	0.16165324435664985
1102	7	0.12395630389284662
1102	8	0.18325183424729374
1102	9	0.17167363403841307
1102	10-11	0.2203161027359215
1102	12-13	0.23420694354737748
1102	14-15	0.2901448583219377
1102	16-17	0.23644946521852717
1102	18-19	0.2735430092648059
1102	20-21	0.1835942211185042
1102	22-23	0.23789395545446723
1102	24-25	0.26994372633486563
1102	26-27	0.3444345959973063
1102	28-29	0.3380255934802534
1102	30-31	0.2620645099649863
1102	32-33	0.2649527338906381
1102	34-35	0.29519180054720096
1102	36-37	0.37934409474380004
1102	38-39	0.4038696403955484
1102	40-41	0.39072888497091895
1102	42-43	0.3796016549373391
1102	44-45	0.3969955085531396
1102	46-47	0.30751020569694276
1102	48-49	0.2603068357345393
1102	50-51	0.3370153191632568
1102	52-53	0.3732624065066972
1102	54-55	0.24151787861779184
1102	56-57	0.325267799530053
1102	58-59	0.3534799848282546
1102	60-61	0.3409214398225586
1102	62-63	0.32854210772342896
1102	64-65	0.3265709461417927
1102	66-67	0.3034273420028839
1102	68-69	0.3373791154693322
1102	70-71	0.34219005697833893
1102	72-73	0.32537330002841713
1102	74-75	0.298077062511922
1102	76-77	0.3307788591484666
1102	78-79	0.3650231087106164
1102	80-81	0.30684818309747897
1102	82-83	0.35466894676573446
1102	84-85	0.3433587926816202
1102	86-87	0.35012835836312917
1102	88-89	0.3315049425454859
1102	90-91	0.37929318553583613
1102	92-93	0.4318018551464107
1102	94-95	0.44204456787267077
1102	96-97	0.4360717640444882
1102	98-99	0.38782143056978313
1102	100	0.44274667925730427
1103	1	0.16900283277054484
1103	2	0.17857804963532686
1103	3	0.31338497092116313
1103	4	0.1886361322980008
1103	5	0.1800654800903274
1103	6	0.15531017320184048
1103	7	0.2127825806018535
1103	8	0.15246974061010832
1103	9	0.2274418702755483
1103	10-11	0.14518596724700217
1103	12-13	0.15898361741969325
1103	14-15	0.21235305850654385
1103	16-17	0.2093962638674256
1103	18-19	0.24930198094192235
1103	20-21	0.2706147768507847
1103	22-23	0.2473175626577131
1103	24-25	0.22028509165591714
1103	26-27	0.18498639906430725
1103	28-29	0.243278684739046
1103	30-31	0.2354111861527528
1103	32-33	0.24047729105502214
1103	34-35	0.24017519713878954
1103	36-37	0.28704344369386803
1103	38-39	0.24415677109288936
1103	40-41	0.24169046583254072
1103	42-43	0.29531574118237813
1103	44-45	0.2687305563787632
1103	46-47	0.2799936325532357
1103	48-49	0.1738384122835086
1103	50-51	0.22855061829822887
1103	52-53	0.2759370271393209
1103	54-55	0.2534136465385117
1103	56-57	0.2036972355783817
1103	58-59	0.26618234956707454
1103	60-61	0.26951615676102847
1103	62-63	0.2646387943326616
1103	64-65	0.31380115821547605
1103	66-67	0.25579418976660406
1103	68-69	0.2949026305966669
1103	70-71	0.3898654770921439
1103	72-73	0.3533427070960329
1103	74-75	0.3461692043211997
1103	76-77	0.34163262426116603
1103	78-79	0.3662139837352498
1103	80-81	0.39859734550467607
1103	82-83	0.4020896777539278
1103	84-85	0.368079174204226
1103	86-87	0.3570584278339055
1103	88-89	0.36653103784156116
1103	90-91	0.3827319611896911
1103	92-93	0.4374742481324745
1103	94-95	0.316677817175119
1103	96-97	0.3579555772701255
1103	98-99	0.45172995469354404
1103	100	0.49042594066048295
1104	1	0.18742006466549554
1104	2	0.2032733264748785
1104	3	0.34121461079810445
1104	4	0.23509420590019658
1104	5	0.1665275307790779
1104	6	0.16145235679010028
1104	7	0.21452303485637714
1104	8	0.20325553618816627
1104	9	0.22590107280704785
1104	10-11	0.23646102911782663
1104	12-13	0.22327543223697432
1104	14-15	0.2680229514177199
1104	16-17	0.31452680259594956
1104	18-19	0.27724687711857854
1104	20-21	0.3028243373174888
1104	22-23	0.305146514750291
1104	24-25	0.32489233701508624
1104	26-27	0.30653581571082356
1104	28-29	0.31389336567621484
1104	30-31	0.3425491259276896
1104	32-33	0.3690784388705737
1104	34-35	0.33014174084296855
1104	36-37	0.3563896258331454
1104	38-39	0.3788305280734363
1104	40-41	0.33751942641563204
1104	42-43	0.45153805799112945
1104	44-45	0.39738708675540835
1104	46-47	0.45706543216729756
1104	48-49	0.4410408777251291
1104	50-51	0.43845106843588155
1104	52-53	0.3930780941716563
1104	54-55	0.4216578490236742
1104	56-57	0.41167233602414655
1104	58-59	0.47410419008321014
1104	60-61	0.4493735607092404
1104	62-63	0.4656784359247865
1104	64-65	0.3986752372458042
1104	66-67	0.4509537539271946
1104	68-69	0.44153575916854493
1104	70-71	0.5308938167071702
1104	72-73	0.4698083825662849
1104	74-75	0.4947623694893224
1104	76-77	0.408705196306002
1104	78-79	0.42377731568058863
1104	80-81	0.4564663040231167
1104	82-83	0.4577175775079425
1104	84-85	0.48373399798517625
1104	86-87	0.49117870218972115
1104	88-89	0.48288397440123987
1104	90-91	0.4223364913925849
1104	92-93	0.5322368064355913
1104	94-95	0.5294124333130057
1104	96-97	0.5804545505118739
1104	98-99	0.649247024662774
1104	100	0.6197704301306004
1105	1	0.18177275145239236
1105	2	0.18880615986955007
1105	3	0.30746875139434593
1105	4	0.16640120299666705
1105	5	0.1387702003901694
1105	6	0.14070911976396872
1105	7	0.15771827163714391
1105	8	0.15205846936610357
1105	9	0.15805088571344328
1105	10-11	0.20927654510768434
1105	12-13	0.15325047364293454
1105	14-15	0.2554781588564836
1105	16-17	0.28609758378844674
1105	18-19	0.2522333307234561
1105	20-21	0.22213127307178837
1105	22-23	0.23940336718148103
1105	24-25	0.21698188124278062
1105	26-27	0.24093420241183594
1105	28-29	0.2707110363096845
1105	30-31	0.20632926026704013
1105	32-33	0.1916906311373694
1105	34-35	0.23155337745197357
1105	36-37	0.2716275312026255
1105	38-39	0.24094356123909932
1105	40-41	0.2296623255486665
1105	42-43	0.2493574717644904
1105	44-45	0.2945325673712844
1105	46-47	0.2949952746201703
1105	48-49	0.28004388860313867
1105	50-51	0.3145386139313189
1105	52-53	0.2922670357125696
1105	54-55	0.28378398129783733
1105	56-57	0.28509948453352507
1105	58-59	0.3348178550731973
1105	60-61	0.3055373621855395
1105	62-63	0.31875595964375947
1105	64-65	0.29931658237727987
1105	66-67	0.3495612228471714
1105	68-69	0.2624757384144516
1105	70-71	0.30889747423385217
1105	72-73	0.3124922429310715
1105	74-75	0.2633145410020177
1105	76-77	0.23319311701102663
1105	78-79	0.24026423701948119
1105	80-81	0.1968767981342765
1105	82-83	0.21559397559816063
1105	84-85	0.28994930132218855
1105	86-87	0.24225762144298102
1105	88-89	0.2104113355634709
1105	90-91	0.2640264169640929
1105	92-93	0.31663634330223545
1105	94-95	0.2600733568193263
1105	96-97	0.35730261107703853
1105	98-99	0.37286861506068547
1105	100	0.3965450078920192
1106	1	0.1937758506087981
1106	2	0.18164964341492862
1106	3	0.28626643493247883
1106	4	0.20256846763360414
1106	5	0.15391769049988824
1106	6	0.15106328036162608
1106	7	0.1871242443337735
1106	8	0.16135803756716172
1106	9	0.2034405981216878
1106	10-11	0.19118202866739864
1106	12-13	0.19091320246656096
1106	14-15	0.2490437561261487
1106	16-17	0.22122752204303708
1106	18-19	0.2550260336497132
1106	20-21	0.2553123025969839
1106	22-23	0.20558949023983786
1106	24-25	0.17799462672630995
1106	26-27	0.258301015665495
1106	28-29	0.25979710402999956
1106	30-31	0.30322301444616073
1106	32-33	0.24235812850348282
1106	34-35	0.27879480562233283
1106	36-37	0.29289663820182454
1106	38-39	0.364241157232577
1106	40-41	0.33529190230419914
1106	42-43	0.3807522176132849
1106	44-45	0.3497109731038037
1106	46-47	0.4331660171762266
1106	48-49	0.4145161876994621
1106	50-51	0.363576217003839
1106	52-53	0.32162915353485033
1106	54-55	0.3609290919474972
1106	56-57	0.3492532224829361
1106	58-59	0.4145842938470281
1106	60-61	0.3345204814432492
1106	62-63	0.28522620191228043
1106	64-65	0.26566930443129166
1106	66-67	0.28569191070290856
1106	68-69	0.3174841254380638
1106	70-71	0.33966544796366094
1106	72-73	0.3869557559586241
1106	74-75	0.38938965167808703
1106	76-77	0.3082360827599331
1106	78-79	0.3537502629862814
1106	80-81	0.3649018495856282
1106	82-83	0.3612954691590957
1106	84-85	0.3547244837131842
1106	86-87	0.4158906252700376
1106	88-89	0.3393495362980943
1106	90-91	0.3816707316931769
1106	92-93	0.4031832715790067
1106	94-95	0.38972153719226554
1106	96-97	0.42796730616503
1106	98-99	0.5191264487982821
1106	100	0.5145874244113955
1107	1	0.11935679128164267
1107	2	0.07464886791988334
1107	3	0.19621048464778568
1107	4	0.1253188686200275
1107	5	0.114857391538564
1107	6	0.08905026936742644
1107	7	0.07281458598006196
1107	8	0.09659714815671094
1107	9	0.13112465700601206
1107	10-11	0.14233789650907624
1107	12-13	0.11737363676905943
1107	14-15	0.21889006543712952
1107	16-17	0.18391286805845652
1107	18-19	0.1722091073417289
1107	20-21	0.1594072288881918
1107	22-23	0.1517381194768177
1107	24-25	0.1345101635645065
1107	26-27	0.2145766556212152
1107	28-29	0.21634026737664414
1107	30-31	0.18542149752684622
1107	32-33	0.2988537007380714
1107	34-35	0.1854996673249545
1107	36-37	0.20183520936799937
1107	38-39	0.2054586097882094
1107	40-41	0.17775301296795476
1107	42-43	0.2394412254908076
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2216	74-75	-0.6650110936934226
2216	76-77	-0.6037043272646088
2216	78-79	-0.8570313138753107
2216	80-81	-1.0185985462098799
2216	82-83	-0.9008814934881322
2216	84-85	-1.0122627887043976
2216	86-87	-0.8012960609635371
2216	88-89	-1.14029635497549
2216	90-91	-0.9727022763340898
2216	92-93	-1.0414353806341055
2216	94-95	-1.0344566189955948
2216	96-97	-1.0718039419811909
2216	98-99	-1.2495664911004631
2216	100	-1.1685627078746421
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	322.0
3	47.0
4	47.0
5	104.0
6	310.0
7	814.0
8	1962.0
9	3512.0
10	5251.0
11	7585.0
12	10829.0
13	14100.0
14	17647.0
15	22284.0
16	28138.0
17	38422.0
18	38017.0
19	22426.0
20	17518.0
21	17777.0
22	19678.0
23	22950.0
24	28130.0
25	34267.0
26	40528.0
27	44942.0
28	57825.0
29	76168.0
30	98190.0
31	127015.0
32	162985.0
33	213356.0
34	286350.0
35	407914.0
36	638546.0
37	1153530.0
38	1708894.0
39	731257.0
40	3644.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.158252859064284	21.63738079651579	15.442897224477727	22.761469119942202
2	14.898461991181463	20.924352000178263	41.104219189645704	23.07296681899457
3	17.270464853248605	26.231005913049067	31.864139960129638	24.63438927357269
4	12.136054123055533	18.34555436366398	39.12514539320434	30.39324612007615
5	12.053828096723713	38.17990684027165	36.40599212128689	13.36027294171774
6	29.138458478316824	39.49238122904713	17.65029334222036	13.718866950415688
7	24.99542459211693	33.91056384262825	23.130050869360268	17.963960695894553
8	23.753313200847355	37.32122806307538	20.768356201799143	18.15710253427812
9	23.85117119791797	17.137995120984925	22.48461114603768	36.526222535059425
10-11	22.944216397704775	27.7695226551096	29.352482705613586	19.933778241572035
12-13	23.749504355362003	25.248846801508236	29.691888114707947	21.309760728421818
14-15	21.056391855192498	27.336806878274366	27.356755184716402	24.250046081816727
16-17	19.87326980356959	30.87489335654052	28.108577992722278	21.143258847167615
18-19	19.72127109399244	28.952589084095465	31.18040829862248	20.145731523289616
20-21	20.778980329162703	28.101280403049284	30.972608387941325	20.147130879846692
22-23	20.454309739302516	28.19450718392288	31.036650614644813	20.314532462129794
24-25	20.45918789502357	28.297045438719376	31.005558491137954	20.238208175119095
26-27	20.216631163881303	28.24931590029422	31.16606865459326	20.367984281231212
28-29	20.123679803908075	28.034853504520534	31.415833505372536	20.42563318619885
30-31	20.500620895547822	28.56980532274362	30.838732478481656	20.090841303226902
32-33	20.440092130977384	28.115931343333717	31.20934810738674	20.234628418302165
34-35	20.401212332325734	27.867930621533315	31.214845729597396	20.516011316543555
36-37	20.466388755877478	28.378875120785512	30.710302784036937	20.444433339300073
38-39	20.350859859717662	28.041975251599023	31.0275831141473	20.579581774536017
40-41	20.228190714751037	27.820569032479625	31.237670547863505	20.713569704905826
42-43	20.300638110685753	27.764651350186043	31.107730035375408	20.826980503752797
44-45	20.2565069259639	28.02258659543923	30.882670032733294	20.838236445863576
46-47	20.49944664949938	28.09266566660241	30.376497075463345	21.031390608434865
48-49	20.391476220425194	28.079239661808664	30.151994070682065	21.377290047084074
50-51	20.31574148374166	28.08192844716813	30.051861691778274	21.550468377311933
52-53	20.171392431403294	28.173152151027853	30.251726745362507	21.40372867220634
54-55	20.276192124804297	28.379749712100182	29.861877722063397	21.482180441032128
56-57	20.359767484441686	28.149702269160805	29.665419926031184	21.825110320366328
58-59	20.07232021244994	28.057568455482823	30.03241867109664	21.837692660970596
60-61	20.35227517845561	28.508069078068583	29.34520249272992	21.79445325074589
62-63	19.938126220511215	28.685680692269333	29.373947227792357	22.00224585942709
64-65	19.967496000900503	28.451763970304995	29.449650505820752	22.131089522973745
66-67	20.02908313968468	28.538015145352208	29.193278763532366	22.239622951430746
68-69	20.429320171241766	28.637306363367053	28.954247487868628	21.97912597752255
70-71	20.37532416090915	28.461841893447886	28.87110694575806	22.291726999884897
72-73	20.22380799651332	28.537006474858156	28.925923968854157	22.313261559774364
74-75	20.474700240201074	28.43363011656142	28.762128843462957	22.329540799774545
76-77	20.599508244438542	28.413486584287956	28.586132715999575	22.40087245527393
78-79	20.605433251393666	28.481587332927255	28.630381361503865	22.282598054175214
80-81	20.479870688719384	28.352112716442168	28.677201472529752	22.490815122308693
82-83	20.395632674833056	28.310874983092848	28.762221177412815	22.53127116466128
84-85	20.51528920480864	28.10822112117831	28.610335047927304	22.76615462608574
86-87	20.62446979462747	28.213614957126843	28.526181648821453	22.635733599424235
88-89	20.457536462366434	28.025687424608503	28.658484433100195	22.858291679924864
90-91	20.369840129514582	27.862585820165226	28.683841063302868	23.083732987017328
92-93	20.402507407075156	27.91544649107608	28.613778122410395	23.068267979438378
94-95	20.30399450241011	27.654284636602295	28.838129469290543	23.203591391697053
96-97	20.44353888963305	27.59804180599198	28.755090161553877	23.203329142821094
98-99	20.42193363446482	27.652374595338348	28.68894744754401	23.236744322652818
100	20.452967290156046	27.618119609160996	28.678796522074236	23.25011657860872
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	639.0
1	476.0
2	509.5
3	707.0
4	476.5
5	354.5
6	941.0
7	2584.0
8	4511.5
9	5363.0
10	4272.5
11	2842.0
12	2735.0
13	3139.5
14	3674.5
15	4224.0
16	4879.0
17	5646.5
18	6653.5
19	8036.0
20	9999.0
21	12757.5
22	16775.5
23	22785.5
24	30337.5
25	39952.0
26	52501.5
27	67184.5
28	85047.0
29	104242.0
30	121777.0
31	137543.0
32	151435.5
33	165230.0
34	179626.5
35	192457.0
36	203016.5
37	214152.0
38	223907.5
39	230641.0
40	235983.5
41	237509.0
42	235408.5
43	233025.0
44	229124.0
45	223252.5
46	217159.5
47	208508.0
48	198480.5
49	188951.0
50	179252.0
51	168539.5
52	154642.5
53	141981.5
54	132248.5
55	122937.5
56	111196.5
57	97553.5
58	85325.5
59	74458.5
60	63692.0
61	52348.0
62	42680.5
63	34692.5
64	27354.5
65	21185.0
66	16496.5
67	12631.0
68	9506.0
69	7142.5
70	5317.0
71	3951.0
72	2673.0
73	1573.0
74	1022.5
75	682.5
76	438.0
77	267.0
78	168.5
79	95.5
80	49.5
81	20.5
82	12.5
83	8.5
84	5.0
85	3.0
86	2.5
87	2.0
88	1.0
89	2.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03537441582650381
2	0.0
3	0.0
4	6.553851936360131E-5
5	0.0
6	0.0
7	0.0
8	7.209237129996145E-4
9	0.0
10-11	0.0
12-13	0.04325542277997687
14-15	9.830777904540197E-5
16-17	0.0
18-19	4.915388952270098E-5
20-21	1.2288472380675246E-4
22-23	0.0
24-25	9.830777904540197E-5
26-27	6.553851936360131E-5
28-29	3.440772266589069E-4
30-31	0.0
32-33	2.2119250285215447E-4
34-35	6.553851936360131E-5
36-37	5.7346204443151145E-5
38-39	2.9492333713620594E-4
40-41	1.4746166856810297E-4
42-43	4.833465803065597E-4
44-45	2.457694476135049E-4
46-47	1.0650009396585214E-4
48-49	8.110391771245664E-4
50-51	4.6696195046565936E-4
52-53	5.898466742724119E-4
54-55	4.833465803065597E-4
56-57	5.48885099670161E-4
58-59	1.3107703872720263E-4
60-61	2.048078730112541E-4
62-63	8.110391771245664E-4
64-65	5.243081549088105E-4
66-67	0.001450039740919679
68-69	0.001589309094567332
70-71	3.850388012611577E-4
72-73	9.585008456926693E-4
74-75	9.9946242029492E-4
76-77	7.45500657760965E-4
78-79	0.0034981184710322203
80-81	0.0029246564266007086
82-83	0.0015483475199650812
84-85	0.0030147718907256606
86-87	0.0016958091885331842
88-89	0.0022774635478851456
90-91	0.0030229642056461108
92-93	0.002678886978987204
94-95	0.0039814650513387805
96-97	0.0034161953218277185
98-99	0.003088502725009712
100	0.0016876168736127337
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6103281.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.89736289640936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.2843648451034	47.98085378984562
2	14.403342709482402	18.11864546639334
3	4.499599782257487	8.490388811797612
4	1.845782431526347	4.64379389694118
5	0.9328058770108095	2.9335514879126143
6	0.5281038581075262	1.9929804006229772
7	0.34501486195836667	1.5190367484074978
8	0.2326537294897333	1.170664484233505
9	0.15799444818821087	0.894369072898066
>10	0.723254525279207	7.7118049334773024
>50	0.032520320551941415	1.3930323578250388
>100	0.013626058167028157	1.4934019591544814
>500	4.943007615801688E-4	0.22296374663784846
>1k	3.121779643872584E-4	0.37210284723152576
>5k	5.2029660593891084E-5	0.27520327947723844
>10k+	7.804449089083662E-5	0.7872067171442976
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT	17454	0.28597732924307434	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15329	0.25115999083116114	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15327	0.2511272215714793	No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	8519	0.1395806616146299	RNA PCR Primer, Index 46 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8300	0.13599242767947273	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.915388952270099E-5	0.0
2	0.0	0.0	0.0	4.915388952270099E-5	0.0
3	0.0	0.0	0.0	4.915388952270099E-5	0.0
4	0.0	0.0	0.0	4.915388952270099E-5	0.0
5	0.0	1.638462984090033E-5	0.0	6.553851936360131E-5	0.0
6	0.0	1.638462984090033E-5	0.0	6.553851936360131E-5	0.0
7	0.0	1.638462984090033E-5	0.0	9.830777904540198E-5	0.0
8	0.0	1.638462984090033E-5	0.0	1.146924088863023E-4	0.0
9	0.0	1.638462984090033E-5	0.0	2.1300018793170427E-4	0.0
10-11	0.0	1.638462984090033E-5	0.0	2.785387072953056E-4	0.0
12-13	0.0	1.638462984090033E-5	0.0	3.358849117384567E-4	0.0
14-15	0.0	3.276925968180066E-5	0.0	3.686541714202574E-4	0.0
16-17	0.0	4.915388952270099E-5	0.0	4.423850057043089E-4	0.0
18-19	0.0	4.915388952270099E-5	0.0	5.406927847497108E-4	0.0
20-21	0.0	4.915388952270099E-5	0.0	8.356161218859168E-4	0.0
22-23	0.0	4.915388952270099E-5	0.0	0.001794116967578586	0.0
24-25	0.0	5.734620444315115E-5	0.0	0.004047003570702381	0.0
26-27	0.0	6.553851936360131E-5	0.0	0.008823123169324827	0.0
28-29	0.0	6.553851936360131E-5	0.0	0.027853870729530557	0.0
30-31	0.0	6.553851936360131E-5	0.0	0.08375822774668248	0.0
32-33	0.0	6.553851936360131E-5	0.0	0.2068641440562871	0.0
34-35	0.0	6.553851936360131E-5	0.0	0.3957379645472656	0.0
36-37	0.0	7.373083428405147E-5	0.0	0.6915378793799598	2.4576944761350495E-5
38-39	0.0	9.011546412495181E-5	0.0	1.065558017073112	3.276925968180066E-5
40-41	0.0	9.830777904540198E-5	0.0	1.4355475358254028	3.276925968180066E-5
42-43	0.0	9.830777904540198E-5	0.0	1.7928717357106776	3.276925968180066E-5
44-45	0.0	9.830777904540198E-5	0.0	2.2192571503753475	3.276925968180066E-5
46-47	0.0	1.0650009396585215E-4	0.0	2.715375549642889	3.276925968180066E-5
48-49	0.0	1.146924088863023E-4	0.0	3.206193521156899	3.276925968180066E-5
50-51	0.0	1.146924088863023E-4	0.0	3.674982357849819	3.276925968180066E-5
52-53	0.0	1.146924088863023E-4	0.0	4.111427935236801	3.276925968180066E-5
54-55	0.0	1.146924088863023E-4	0.0	4.576456499381234	3.276925968180066E-5
56-57	0.0	1.146924088863023E-4	0.0	5.103615579882362	3.276925968180066E-5
58-59	0.0	1.146924088863023E-4	0.0	5.652377794828716	3.276925968180066E-5
60-61	0.0	1.146924088863023E-4	0.0	6.180847645717115	3.276925968180066E-5
62-63	0.0	1.146924088863023E-4	0.0	6.683716512479108	3.276925968180066E-5
64-65	0.0	1.146924088863023E-4	0.0	7.196252310847232	3.276925968180066E-5
66-67	0.0	1.146924088863023E-4	0.0	7.755361419538114	3.276925968180066E-5
68-69	0.0	1.146924088863023E-4	0.0	8.347125095501912	3.276925968180066E-5
70-71	0.0	1.146924088863023E-4	0.0	8.939904618515843	3.276925968180066E-5
72-73	0.0	1.4746166856810294E-4	0.0	9.522812402050636	3.276925968180066E-5
74-75	0.0	1.6384629840900328E-4	0.0	10.087312053959174	3.276925968180066E-5
76-77	0.0	1.6384629840900328E-4	0.0	10.677363536104597	3.276925968180066E-5
78-79	0.0	1.6384629840900328E-4	0.0	11.30309254972858	3.276925968180066E-5
80-81	0.0	1.6384629840900328E-4	0.0	11.945591231994726	3.276925968180066E-5
82-83	0.0	1.6384629840900328E-4	0.0	12.575580249377342	3.276925968180066E-5
84-85	0.0	1.6384629840900328E-4	0.0	13.175413355537785	3.276925968180066E-5
86-87	0.0	1.8842324317035376E-4	0.0	13.787674203432548	3.276925968180066E-5
88	0.0	2.1300018793170427E-4	0.0	14.260870505552669	3.276925968180066E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10580	0.0	58.16867	1
GTATCAA	19485	0.0	40.608677	1
ATCAACG	23990	0.0	32.441017	3
TCAACGC	24165	0.0	32.20608	4
TATCAAC	25165	0.0	31.150389	2
GTGGTAT	4510	0.0	31.065252	1
CAACGCA	25090	0.0	31.0	5
AACGCAG	26080	0.0	29.883024	6
TGGTATC	4570	0.0	28.794277	2
ACGCAGA	29115	0.0	26.477182	7
CGCAGAG	29080	0.0	26.266441	8
GCAGAGT	31335	0.0	23.805622	9
GTACATG	15060	0.0	21.946512	1
GAGTACT	22665	0.0	21.469395	12-13
TAGACCG	265	9.076757E-10	21.281378	5
AGAGTAC	29130	0.0	21.022121	10-11
CAGAGTA	31035	0.0	20.906244	10-11
TACATGG	14995	0.0	20.340544	2
AGTACTT	23740	0.0	19.695148	12-13
ACATGGG	15170	0.0	19.269442	3
>>END_MODULE