FastQCFastQC Report
Wed 25 May 2016
SRR2049349_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2049349_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5597736
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52050.044.1677131
GTATCAA89500.035.5091631
TCAACGC94300.032.0539174
ATCAACG94950.031.9335063
CAACGCA100700.029.923635
AACGCAG106700.028.324516
TATCAAC110950.027.8368382
GTACATG143000.026.6954211
TACATGG142700.025.0693192
ACGCAGA122950.024.2748857
CGCAGAG125100.023.7074058
ACATGGG146100.023.681513
CATGGGG102350.020.4846654
GCAGAGT145050.019.5071859
GTCGTAC4150.019.2582861
GTGGTAT35850.018.4904171
AGAGTAC122300.017.71700510-11
TATACTG35500.016.9499075
CATGGGA48000.016.7469884
CAGAGTA137900.016.68415810-11
Produced by FastQC (version 0.11.5)